RLG00000003624

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0010128
Physical Location & Seq
Reverse (-)
50891022 .. 50894734
3713 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000003624

Sequence Viewer

Length: 2349 bp
ATGGCTACAGAGTCAGTCAGAGTAGTCCACAGTTTCAGCTTCAATGGGTCTCAGACAAGGCCTACCATCCCTCTCTCCCCAGCTCGCTTTCTGGGTCTTCGTCCTCCTCGTTCTTCTTCTTCTTCTTCTCTCACCTCATCTTCACTTTCCCAGTTCTTTGGGAATCTGCGTCTCACCTCCAACTCCTCAAAGACCTCTCATTTGCGCCAACACAGCCGAAGAAACCTCTCTGTTTTCGCCATGGCCGCCGATGATGGAAAGCGTGCAGTACCATTGGTAGATTATCGGAATATTGGAATTATGGCTCACATAGATGCAGGGAAGACTACTACAACTGAACGAATTTTGTACTATACTGGAAGAAACTATAAAATTGGGGAGGTGCATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCAGCAACTACAACATTTTGGGACAAACACAGAATTAACATTATTGATACTCCTGGCCATGTGGACTTCACCCTCGAAGTGGAGCGAGCTCTCAGGGTACTTGATGGTGCAATATGCTTGTTTGACAGTGTTGCTGGGGTGGAACCTCAGTCTGAAACTGTGTGGAGGCAAGCTGATAAATATGGGGTGCCGAGGATTTGCTTTGTTAACAAAATGGATCGTCTTGGGGCAAACTTTTTCCGCACAAGGGACATGATAGTGACAAATTTGGGTGCCAAACCACTTGTACTTCAAATTCCAGTTGGTGCCGAAGATAACTTTAAGGGAGTTATTGATCTTGTGAAGATGAGAGCTATACTTTGGTCAGGAGAAGAATTGGGTGCAAAATTTTCGTATGAGGATATTCCATCTGATCTTCAGGAGTTGGCTGACGAATACAGGTCACTGATGATAGAAACCATAGTTGAGTTGGATGATGAAGCTATGGAGGGCTATCTAGAAGGAGTTGAACCTGATGAGGCCACCATTAAGAAATTGATTAGGCAGGGAACCATCTCAGCTAGTTTTGTGCCAGTATTATGTGGTTCAGCTTTTAAAAATAAGGGGGTTCAGCCATTGCTTGATGCTGTCGTGGATTATTTACCTTCACCGCTTGATGTGCCACCAATGCAGGGAACTGATGCAGATAACCCAGAAATCACGATTGAGAGGGCTGCAAGTGATGATGAACCATTTGCTGGGCTGGCATTCAAGATCATGAGTGATCCATTTGTGGGATCCCTTACATTTGTGAGAATCTATGCAGGAAAGCTAACTGCTGGATCATACGTGCTGAATGCAAACAAGGGGAAAAAGGAGAGGATTGGTAGACTTCTAGAAATGCATGCAAACAGTAGGGAGGATGTTAAGGTTGCTTTAGCTGGTGATATTGTTGCTCTTGCAGGTCTAAAAGATACTGTTACTGGTGAAACATTGAGTGACCCTGAGCATCCTATTGTGCTTGAACGTATGGAATTCCCTGATCCTGTGATTAAGGTGGCAATTGAACCCAAAACTAAAGCCGATGTTGATAAGATGGGAGCTGGTTTGGTTAAACTTGCTCAGGAAGACCCTTCATTCCACTTCTCAAGGGATGAAGAGGTCAATCAGACAGTGATCGAAGGAATGGGTGAATTGCATCTTGAGATTATTGTTGATCGCCTCAAGAGGGAATTCAAGGTCGAGGCAAATGTTGGAGCACCCCAGGTAAACTACCGCGAAAGCATTTCTAAAGTTGCAGAAGTAAAATATGTGCACAAGAAACAATCTGGCGGACAAGGTCAATTCGCAGATATCACAATCCGATTTGAACCCATGGAGCCTGGTAATGGATACGAATTCAAGAGTGAAATCAAGGGAGGTGCAGTGCCAAGAGAGTACATTCCTGGGGTGATGAAAGGACTAGAGGAGTGTATGAGTAACGGTGTGCTTGCAGGCTTTCCTGTCGTCGACGTGCGTGCTGTACTAGTTGATGGTTCTTACCACGATGTTGACTCGAGTGTTTTGGCATTCCAATTGGCAGCTAGAGGAGCTTTCCGAGAGGGTATGAAGAGAGCCGGCCCAAAAATGCTTGAACCTATTATGAAAGTTGAAGTAGTTACACCTGAAGAACATTTGGGAGACGTAATTGGTGACCTTAACTCCAGGAGAGGTCAAATCAACAGCTTTAGTGACAAACCTGGTGGTCTCAAGGTGGTTGATTCTTTGGTTCCTCTTGCGGAGATGTTCCAATATGTTAGTACACTCAGGGGCATGACAAAAGGTCGTGCATCATACACGATGCAATTAGCTAGGTTTGATGTTGTTCCTCAGCACATTCAGAACCAACTTGCCGCTAAGGAGGAAGAAGTTGCTGCTTGA

Protein Analysis

783

Amino Acids

86.08

Weight (kDa)

5.36

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 95 - 366 2.3e-67 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 394 - 476 1.4e-16 Elongation factor G domain 2
GTP_EFTU_D2 PF03144 409 - 475 1.8e-16 Elongation factor Tu domain 2
EFG_III PF14492 489 - 562 7.1e-32 Elongation Factor G, domain III
RF3_C PF16658 499 - 577 2.1e-06 Class II release factor RF3, C-terminal domain
EFG_IV PF03764 564 - 682 4.6e-46 Elongation factor G, domain IV
EFG_C PF00679 686 - 772 4.5e-26 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1382
AccB1I GGYRCC 3 cut(s) 637, 722, 755
AccB7I CCANNNNNTGG 1 cut(s) 1187
AccI GTMKAC 2 cut(s) 1318, 1938
AccII CGCG 1 cut(s) 1707
AciI CCGC 7 cut(s) 246, 691, 1100, 1705, 1761, 2207, 2322
AclWI GGATC 6 cut(s) 675, 1208, 1221, 1234, 1279, 1466
AcoI YGGCCR 2 cut(s) 243, 505
AcsI RAATTY 7 cut(s) 342, 715, 744, 836, 1463, 1661, 1826
AcuI CTGAAG 2 cut(s) 851, 2115
AfaI GTAC 7 cut(s) 270, 350, 549, 738, 1868, 1953, 2230
AfiI CCNNNNNNNGG 7 cut(s) 529, 697, 1121, 1187, 1223, 1657, 1817
AhdI GACNNNNNGTC 1 cut(s) 2250
AhlI ACTAGT 1 cut(s) 1954
AjiI CACGTC 1 cut(s) 1942
AjnI CCWGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
Alw21I GWGCWC 3 cut(s) 541, 1690, 1746
Alw26I GTCTC 4 cut(s) 54, 176, 2103, 2180
Alw44I GTGCAC 1 cut(s) 1742
AlwI GGATC 6 cut(s) 675, 1208, 1221, 1234, 1279, 1466
Ama87I CYCGRG 1 cut(s) 1984
AoxI GGCC 5 cut(s) 59, 243, 505, 969, 2047
ApaLI GTGCAC 1 cut(s) 1742
ApeKI GCWGC 4 cut(s) 452, 1163, 2009, 2342
ApoI RAATTY 7 cut(s) 342, 715, 744, 836, 1463, 1661, 1826
ArsI GACNNNNNNTTYG 2 cut(s) 184, 216
AspLEI GCGC 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 2048
AsuHPI GGTGA 9 cut(s) 124, 166, 511, 1089, 1385, 1427, 1631, 1891, 2132
AvaI CYCGRG 1 cut(s) 1984
BaeGI GKGCMC 1 cut(s) 1746
BalI TGGCCA 1 cut(s) 507
BamHI GGATCC 1 cut(s) 1226
BanI GGYRCC 3 cut(s) 637, 722, 755
BanII GRGCYC 1 cut(s) 541
BbsI GAAGAC 3 cut(s) 89, 329, 1563
Bbv12I GWGCWC 3 cut(s) 541, 1690, 1746
BbvCI CCTCAGC 1 cut(s) 2298
BbvI GCAGC 4 cut(s) 464, 1150, 2021, 2329
BccI CCATC 8 cut(s) 74, 248, 406, 548, 865, 1010, 1519, 1955
BcgI CGANNNNNNTGC 4 cut(s) 822, 856, 1928, 1962
BciT130I CCWGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
BciVI GTATCC 1 cut(s) 1814
BcoDI GTCTC 4 cut(s) 54, 176, 2103, 2180
BcuI ACTAGT 1 cut(s) 1954
BfaI CTAG 7 cut(s) 947, 1011, 1325, 1892, 1955, 2013, 2280
BfmI CTRYAG 2 cut(s) 6, 450
BfuAI ACCTGC 1 cut(s) 1382
BfuI GTATCC 1 cut(s) 1814
BisI GCNGC 6 cut(s) 246, 453, 1164, 2010, 2322, 2343
BlsI GCNGC 6 cut(s) 247, 454, 1165, 2011, 2323, 2344
Bme1390I CCNGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
BmeRI GACNNNNNGTC 1 cut(s) 2250
BmeT110I CYCGRG 1 cut(s) 1984
BmgBI CACGTC 1 cut(s) 1942
BmgT120I GGNCC 1 cut(s) 2048
BmiI GGNNCC 8 cut(s) 594, 639, 724, 757, 1000, 1228, 1809, 2199
BmrFI CCNGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
BmrI ACTGGG 1 cut(s) 145
BmsI GCATC 7 cut(s) 304, 1063, 1120, 1447, 1636, 2259, 2267
BmuI ACTGGG 1 cut(s) 145
BpiI GAAGAC 3 cut(s) 89, 329, 1563
BpmI CTGGAG 1 cut(s) 2116
Bpu10I CCTNAGC 4 cut(s) 1434, 1551, 2298, 2325
BpuEI CTTGAG 4 cut(s) 1561, 1637, 1652, 2162
BsaAI YACGTR 1 cut(s) 1279
BsaI GGTCTC 2 cut(s) 54, 2180
BsaJI CCNNGG 5 cut(s) 240, 641, 1692, 1803, 1874
BsaXI ACNNNNNCTCC 4 cut(s) 1839, 1869, 2114, 2144
Bsc4I CCNNNNNNNGG 7 cut(s) 529, 697, 1121, 1187, 1223, 1657, 1817
Bse118I RCCGGY 1 cut(s) 2045
Bse1I ACTGG 6 cut(s) 151, 361, 413, 749, 1022, 1417
Bse3DI GCAATG 1 cut(s) 1064
BseBI CCWGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
BseDI CCNNGG 5 cut(s) 240, 641, 1692, 1803, 1874
BseGI GGATG 6 cut(s) 66, 417, 928, 1357, 1438, 1588
BseLI CCNNNNNNNGG 7 cut(s) 529, 697, 1121, 1187, 1223, 1657, 1817
BseMI GCAATG 1 cut(s) 1064
BseMII CTCAG 8 cut(s) 65, 556, 611, 1020, 1425, 1565, 2248, 2312
BseNI ACTGG 6 cut(s) 151, 361, 413, 749, 1022, 1417
BseRI GAGGAG 4 cut(s) 96, 175, 1910, 2031
BseSI GKGCMC 1 cut(s) 1746
BseXI GCAGC 4 cut(s) 464, 1150, 2021, 2329
BseYI CCCAGC 3 cut(s) 79, 584, 1187
BsgI GTGCAG 2 cut(s) 285, 1872
Bsh1236I CGCG 1 cut(s) 1707
BshFI GGCC 5 cut(s) 61, 245, 507, 971, 2049
BshNI GGYRCC 3 cut(s) 637, 722, 755
BsiHKAI GWGCWC 3 cut(s) 541, 1690, 1746
BsiHKCI CYCGRG 1 cut(s) 1984
BsiSI CCGG 1 cut(s) 2046
BslFI GGGAC 2 cut(s) 485, 713
BslI CCNNNNNNNGG 7 cut(s) 529, 697, 1121, 1187, 1223, 1657, 1817
BsmAI GTCTC 4 cut(s) 54, 176, 2103, 2180
BsmBI CGTCTC 2 cut(s) 176, 2103
BsmFI GGGAC 2 cut(s) 485, 713
BsmI GAATGC 3 cut(s) 1196, 1291, 1997
BsnI GGCC 5 cut(s) 61, 245, 507, 971, 2049
Bso31I GGTCTC 2 cut(s) 54, 2180
BsoBI CYCGRG 1 cut(s) 1984
Bsp1286I GDGCHC 3 cut(s) 541, 1690, 1746
Bsp19I CCATGG 2 cut(s) 240, 1803
BspACI CCGC 7 cut(s) 246, 691, 1100, 1705, 1761, 2207, 2322
BspANI GGCC 5 cut(s) 61, 245, 507, 971, 2049
BspCNI CTCAG 8 cut(s) 64, 555, 610, 1019, 1426, 1564, 2247, 2311
BspFNI CGCG 1 cut(s) 1707
BspHI TCATGA 1 cut(s) 1206
BspLI GGNNCC 8 cut(s) 594, 639, 724, 757, 1000, 1228, 1809, 2199
BspMAI CTGCAG 1 cut(s) 454
BspMI ACCTGC 1 cut(s) 1382
BspPI GGATC 6 cut(s) 675, 1208, 1221, 1234, 1279, 1466
BspT107I GGYRCC 3 cut(s) 637, 722, 755
BspTNI GGTCTC 2 cut(s) 54, 2180
BsrDI GCAATG 1 cut(s) 1064
BsrFI RCCGGY 1 cut(s) 2045
BsrI ACTGG 6 cut(s) 151, 361, 413, 749, 1022, 1417
BssAI RCCGGY 1 cut(s) 2045
BssECI CCNNGG 5 cut(s) 240, 641, 1692, 1803, 1874
BssT1I CCWWGG 2 cut(s) 240, 1803
Bst2UI CCWGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
Bst4CI ACNGT 7 cut(s) 32, 578, 610, 1343, 1408, 1603, 1913
Bst6I CTCTTC 2 cut(s) 1581, 2033
BstBAI YACGTR 1 cut(s) 1279
BstDEI CTNAG 9 cut(s) 51, 542, 597, 1006, 1434, 1551, 2234, 2298, 2325
BstDSI CCRYGG 2 cut(s) 240, 1803
BstEII GGTNACC 1 cut(s) 2120
BstF5I GGATG 6 cut(s) 66, 417, 928, 1357, 1438, 1588
BstFNI CGCG 1 cut(s) 1707
BstHHI GCGC 1 cut(s) 207
BstMAI GTCTC 4 cut(s) 54, 176, 2103, 2180
BstMWI GCNNNNNNNGC 6 cut(s) 213, 245, 1108, 1117, 1193, 2018
BstNI CCWGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
BstNSI RCATGY 1 cut(s) 1337
BstPI GGTNACC 1 cut(s) 2120
BstSCI CCNGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
BstSFI CTRYAG 2 cut(s) 6, 450
BstSLI GKGCMC 1 cut(s) 1746
BstUI CGCG 1 cut(s) 1707
BstV1I GCAGC 4 cut(s) 464, 1150, 2021, 2329
BstV2I GAAGAC 3 cut(s) 89, 329, 1563
BstX2I RGATCY 1 cut(s) 1226
BstXI CCANNNNNNTGG 1 cut(s) 158
BstYI RGATCY 1 cut(s) 1226
BsuI GTATCC 1 cut(s) 1814
BsuRI GGCC 5 cut(s) 61, 245, 507, 971, 2049
BtgI CCRYGG 2 cut(s) 240, 1803
BtrI CACGTC 1 cut(s) 1942
BtsCI GGATG 6 cut(s) 66, 417, 928, 1357, 1438, 1588
BtsI GCAGTG 1 cut(s) 1860
BtsIMutI CAGTG 4 cut(s) 583, 893, 1608, 1860
BveI ACCTGC 1 cut(s) 1382
CciI TCATGA 1 cut(s) 1206
CfoI GCGC 1 cut(s) 207
Cfr10I RCCGGY 1 cut(s) 2045
Cfr13I GGNCC 1 cut(s) 2048
CseI GACGC 1 cut(s) 158
CsiI ACCWGGT 1 cut(s) 2167
Csp6I GTAC 7 cut(s) 269, 349, 548, 737, 1867, 1952, 2229
CspCI CAANNNNNGTGG 2 cut(s) 2152, 2187
CviAII CATG 8 cut(s) 241, 386, 509, 703, 1207, 1334, 1804, 2242
CviQI GTAC 7 cut(s) 269, 349, 548, 737, 1867, 1952, 2229
DdeI CTNAG 9 cut(s) 51, 542, 597, 1006, 1434, 1551, 2234, 2298, 2325
DraI TTTAAA 1 cut(s) 1045
DriI GACNNNNNGTC 1 cut(s) 2250
EaeI YGGCCR 2 cut(s) 243, 505
Eam1104I CTCTTC 2 cut(s) 1581, 2033
Eam1105I GACNNNNNGTC 1 cut(s) 2250
EarI CTCTTC 2 cut(s) 1581, 2033
EciI GGCGGA 1 cut(s) 1776
Ecl136II GAGCTC 1 cut(s) 539
Eco130I CCWWGG 2 cut(s) 240, 1803
Eco147I AGGCCT 1 cut(s) 61
Eco24I GRGCYC 1 cut(s) 541
Eco31I GGTCTC 2 cut(s) 54, 2180
Eco32I GATATC 1 cut(s) 1783
Eco53kI GAGCTC 1 cut(s) 539
Eco57I CTGAAG 2 cut(s) 851, 2115
Eco88I CYCGRG 1 cut(s) 1984
Eco91I GGTNACC 1 cut(s) 2120
EcoICRI GAGCTC 1 cut(s) 539
EcoO65I GGTNACC 1 cut(s) 2120
EcoRI GAATTC 3 cut(s) 1463, 1661, 1826
EcoRII CCWGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
EcoRV GATATC 1 cut(s) 1783
EcoT14I CCWWGG 2 cut(s) 240, 1803
EcoT22I ATGCAT 1 cut(s) 1335
EcoT38I GRGCYC 1 cut(s) 541
ErhI CCWWGG 2 cut(s) 240, 1803
Esp3I CGTCTC 2 cut(s) 176, 2103
FaeI CATG 8 cut(s) 244, 389, 512, 706, 1210, 1337, 1807, 2245
FaqI GGGAC 2 cut(s) 485, 713
FatI CATG 8 cut(s) 240, 385, 508, 702, 1206, 1333, 1803, 2241
FblI GTMKAC 2 cut(s) 1318, 1938
Fnu4HI GCNGC 6 cut(s) 246, 453, 1164, 2010, 2322, 2343
FokI GGATG 6 cut(s) 53, 424, 935, 1364, 1425, 1595
FriOI GRGCYC 1 cut(s) 541
Fsp4HI GCNGC 6 cut(s) 246, 453, 1164, 2010, 2322, 2343
FspBI CTAG 7 cut(s) 947, 1011, 1325, 1892, 1955, 2013, 2280
GlaI GCGC 1 cut(s) 206
GluI GCNGC 6 cut(s) 246, 453, 1164, 2010, 2322, 2343
GsaI CCCAGC 3 cut(s) 83, 588, 1191
GsuI CTGGAG 1 cut(s) 2116
HaeIII GGCC 5 cut(s) 61, 245, 507, 971, 2049
HapII CCGG 1 cut(s) 2046
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 207
Hin1II CATG 8 cut(s) 244, 389, 512, 706, 1210, 1337, 1807, 2245
Hin6I GCGC 1 cut(s) 205
HinP1I GCGC 1 cut(s) 205
HincII GTYRAC 3 cut(s) 658, 1939, 1981
HindII GTYRAC 3 cut(s) 658, 1939, 1981
HinfI GANTC 5 cut(s) 11, 163, 1245, 1982, 2189
HpaI GTTAAC 1 cut(s) 658
HpaII CCGG 1 cut(s) 2046
HphI GGTGA 9 cut(s) 124, 166, 511, 1089, 1385, 1427, 1631, 1891, 2132
Hpy166II GTNNAC 9 cut(s) 28, 514, 658, 1319, 1699, 1744, 1939, 1981, 2231
Hpy188I TCNGA 9 cut(s) 20, 54, 288, 604, 862, 1599, 1793, 2027, 2310
Hpy8I GTNNAC 9 cut(s) 28, 514, 658, 1319, 1699, 1744, 1939, 1981, 2231
Hpy99I CGWCG 2 cut(s) 1940, 1943
HpyAV CCTTC 5 cut(s) 383, 944, 1104, 1572, 1604
HpyCH4III ACNGT 7 cut(s) 32, 578, 610, 1343, 1408, 1603, 1913
HpyCH4IV ACGT 4 cut(s) 1278, 1456, 1941, 2112
HpyF10VI GCNNNNNNNGC 6 cut(s) 213, 245, 1108, 1117, 1193, 2018
HpyF3I CTNAG 9 cut(s) 51, 542, 597, 1006, 1434, 1551, 2234, 2298, 2325
HpySE526I ACGT 4 cut(s) 1278, 1456, 1941, 2112
Hsp92II CATG 8 cut(s) 244, 389, 512, 706, 1210, 1337, 1807, 2245
HspAI GCGC 1 cut(s) 205
KroI GCCGGC 1 cut(s) 2045
KroNI GCCGGC 1 cut(s) 2047
KspAI GTTAAC 1 cut(s) 658
LmnI GCTCC 6 cut(s) 415, 532, 1529, 1685, 1807, 2018
Lsp1109I GCAGC 4 cut(s) 464, 1150, 2021, 2329
LweI GCATC 7 cut(s) 304, 1063, 1120, 1447, 1636, 2259, 2267
MabI ACCWGGT 1 cut(s) 2167
MaeI CTAG 7 cut(s) 947, 1011, 1325, 1892, 1955, 2013, 2280
MaeII ACGT 4 cut(s) 1278, 1456, 1941, 2112
MaeIII GTNAC 8 cut(s) 709, 891, 1408, 1427, 1907, 2086, 2120, 2159
MfeI CAATTG 2 cut(s) 1491, 2003
MflI RGATCY 1 cut(s) 1226
MhlI GDGCHC 3 cut(s) 541, 1690, 1746
MlsI TGGCCA 1 cut(s) 507
MluNI TGGCCA 1 cut(s) 507
MlyI GAGTC 2 cut(s) 20, 1976
MmeI TCCRAC 3 cut(s) 204, 900, 1663
Mox20I TGGCCA 1 cut(s) 507
Mph1103I ATGCAT 1 cut(s) 1335
MroNI GCCGGC 1 cut(s) 2045
MscI TGGCCA 1 cut(s) 507
MseI TTAA 9 cut(s) 486, 657, 771, 978, 1044, 1356, 1482, 1542, 2127
MslI CAYNNNNRTG 2 cut(s) 312, 707
Msp20I TGGCCA 1 cut(s) 507
MspI CCGG 1 cut(s) 2046
MspR9I CCNGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
MunI CAATTG 2 cut(s) 1491, 2003
Mva1269I GAATGC 3 cut(s) 1196, 1291, 1997
MvaI CCWGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
MvnI CGCG 1 cut(s) 1707
MwoI GCNNNNNNNGC 6 cut(s) 213, 245, 1108, 1117, 1193, 2018
NaeI GCCGGC 1 cut(s) 2047
NcoI CCATGG 2 cut(s) 240, 1803
NgoMIV GCCGGC 1 cut(s) 2045
NlaIII CATG 8 cut(s) 244, 389, 512, 706, 1210, 1337, 1807, 2245
NlaIV GGNNCC 8 cut(s) 594, 639, 724, 757, 1000, 1228, 1809, 2199
NmeAIII GCCGAG 1 cut(s) 666
NmuCI GTSAC 5 cut(s) 709, 891, 1427, 2120, 2159
NsiI ATGCAT 1 cut(s) 1335
NspI RCATGY 1 cut(s) 1337
PaeI GCATGC 1 cut(s) 1337
PaeR7I CTCGAG 1 cut(s) 1984
PagI TCATGA 1 cut(s) 1206
PceI AGGCCT 1 cut(s) 61
PcsI WCGNNNNNNNCGW 1 cut(s) 106
PctI GAATGC 3 cut(s) 1196, 1291, 1997
PdiI GCCGGC 1 cut(s) 2047
PfeI GAWTC 3 cut(s) 163, 1245, 2189
PflFI GACNNNGTC 1 cut(s) 1767
PflMI CCANNNNNTGG 1 cut(s) 1187
PfoI TCCNGGA 1 cut(s) 2132
PkrI GCNGC 6 cut(s) 247, 454, 1165, 2011, 2323, 2344
PleI GAGTC 2 cut(s) 19, 1976
PpsI GAGTC 2 cut(s) 19, 1976
Ppu21I YACGTR 1 cut(s) 1279
Psp124BI GAGCTC 1 cut(s) 541
Psp6I CCWGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
PspEI GGTNACC 1 cut(s) 2120
PspFI CCCAGC 3 cut(s) 79, 584, 1187
PspGI CCWGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
PspN4I GGNNCC 8 cut(s) 594, 639, 724, 757, 1000, 1228, 1809, 2199
PspPI GGNCC 1 cut(s) 2048
PspXI VCTCGAGB 1 cut(s) 1984
PstI CTGCAG 1 cut(s) 454
PsuI RGATCY 1 cut(s) 1226
PsyI GACNNNGTC 1 cut(s) 1767
RsaI GTAC 7 cut(s) 270, 350, 549, 738, 1868, 1953, 2230
RsaNI GTAC 7 cut(s) 269, 349, 548, 737, 1867, 1952, 2229
RseI CAYNNNNRTG 2 cut(s) 312, 707
SacI GAGCTC 1 cut(s) 541
SalI GTCGAC 1 cut(s) 1937
SaqAI TTAA 9 cut(s) 486, 657, 771, 978, 1044, 1356, 1482, 1542, 2127
SatI GCNGC 6 cut(s) 246, 453, 1164, 2010, 2322, 2343
Sau96I GGNCC 1 cut(s) 2048
SchI GAGTC 2 cut(s) 20, 1976
ScrFI CCNGG 6 cut(s) 504, 1694, 1812, 1875, 2134, 2169
SduI GDGCHC 3 cut(s) 541, 1690, 1746
SexAI ACCWGGT 1 cut(s) 2167
SfaNI GCATC 7 cut(s) 304, 1063, 1120, 1447, 1636, 2259, 2267
SfcI CTRYAG 2 cut(s) 6, 450
Sfr274I CTCGAG 1 cut(s) 1984
SgrDI CGTCGACG 1 cut(s) 1937
SlaI CTCGAG 1 cut(s) 1984
SmiMI CAYNNNNRTG 2 cut(s) 312, 707
SmlI CTYRAG 5 cut(s) 1576, 1631, 1652, 1984, 2177
SmoI CTYRAG 5 cut(s) 1576, 1631, 1652, 1984, 2177
SpeI ACTAGT 1 cut(s) 1954
SphI GCATGC 1 cut(s) 1337
SseBI AGGCCT 1 cut(s) 61
SsiI CCGC 7 cut(s) 246, 691, 1100, 1705, 1761, 2207, 2322
SspI AATATT 1 cut(s) 292
SspMI CTAG 7 cut(s) 947, 1011, 1325, 1892, 1955, 2013, 2280
SstI GAGCTC 1 cut(s) 541
StuI AGGCCT 1 cut(s) 61
StyD4I CCNGG 6 cut(s) 502, 1692, 1810, 1873, 2132, 2167
StyI CCWWGG 2 cut(s) 240, 1803
TaaI ACNGT 7 cut(s) 32, 578, 610, 1343, 1408, 1603, 1913
TaiI ACGT 4 cut(s) 1281, 1459, 1944, 2115
TaqI TCGA 5 cut(s) 525, 1608, 1671, 1938, 1985
TatI WGTACW 5 cut(s) 348, 736, 1866, 1951, 2228
TauI GCSGC 2 cut(s) 248, 2324
TfiI GAWTC 3 cut(s) 163, 1245, 2189
Tru1I TTAA 9 cut(s) 486, 657, 771, 978, 1044, 1356, 1482, 1542, 2127
Tru9I TTAA 9 cut(s) 486, 657, 771, 978, 1044, 1356, 1482, 1542, 2127
TscAI CASTG 4 cut(s) 583, 900, 1608, 1860
TseFI GTSAC 5 cut(s) 709, 891, 1427, 2120, 2159
TseI GCWGC 4 cut(s) 452, 1163, 2009, 2342
Tsp45I GTSAC 5 cut(s) 709, 891, 1427, 2120, 2159
TspDTI ATGAA 8 cut(s) 402, 942, 1191, 1554, 1599, 1898, 2051, 2087
TspRI CASTG 4 cut(s) 583, 900, 1608, 1860
Tth111I GACNNNGTC 1 cut(s) 1767
Van91I CCANNNNNTGG 1 cut(s) 1187
VneI GTGCAC 1 cut(s) 1742
XapI RAATTY 7 cut(s) 342, 715, 744, 836, 1463, 1661, 1826
XbaI TCTAGA 2 cut(s) 946, 1324
XceI RCATGY 1 cut(s) 1337
XcmI CCANNNNNNNNNTGG 1 cut(s) 916
XhoI CTCGAG 1 cut(s) 1984
XmiI GTMKAC 2 cut(s) 1318, 1938
XspI CTAG 7 cut(s) 947, 1011, 1325, 1892, 1955, 2013, 2280
Zsp2I ATGCAT 1 cut(s) 1335
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.