AT1G62960

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008719
Physical Location & Seq
Reverse (-)
23318187 .. 23320427
2241 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G62960.1

Sequence Viewer

Length: 1674 bp
ATGACCCGTACCGAACCAAACCGGAGCCGGAGCTCCAATTCCGATTCCGATAAGAATTCCGGTAACGTCGGCGGAGGTAGAACCACCGGGATGAGGGTTATTGTTCCGTTACAAGGTGTGGTACAAGGTCGTGGTGGTTTATTCTTAGGCTCTGTGATTCCTTGTGCTTTCTTCTACTTTCTTCAGTTTTACCTAAAACGAAATCGTAAAAACGACGAATCAGATAATTCCGGTGAACAAAACTCCTCAGCTTCTTCTTCTTCTTCTCCTAATTCGGGTTTACCGGATCCCACCCGGTCACAATCCGCTGGTCATCTCACGGAGCTTACTGGTTTACCTCGTTCTCTCTCTCGTATTCTCCTCTCGCCGAGAAATTCCGGTGGAGCTGTTTCGGTTTCGGGTCGGGTTAATTGTGTACTCAAAGGTGGAGATTCTTCGCCTTACTACGTTGGTCAAAAACGGGTCGAGGATGATCCGTATGATGAGTTGGGTAACCCGGACGGAGTTATCCAACTTGGTTTAGCTCAAAACAACAAGTTGAGTTTGGATGATTGGGTTTTAGAGAATCCAAAAGAAGCAATTTCAGATGGATTGAGTATTAGTGGCATTGCTTCTTATGAGCCTTCTGATGGACTTTTGGAACTGAAAATGGCTGTGGCAGGATTTATGACTGAAGCTACCAAAAACTCGGTGACTTTTGATCCATCACAGTTAGTGTTAACTTCTGGTGCATCATCTGCTATTGAGATTCTTTCCTTCTGCTTAGCTGATTCGGGAAACGCCTTCCTTGTTCCAACTCCGTGTTCTCCCGGATATGATAGGGATGTTAAATGGCGAACAGGAGTTGACATTATACACGTTCCATGTAGAAGTGCGGATAATTTCAATATGTCGATGGTCGTGCTTGATCGAGCATTCTATCAAGCTAAGAAACGAGGTGTAAGAATCCGCGGCATTATAATCTCGAATCCTTCAAATCCCATGGGAAGCCTACTGAGCAGAGAGAATCTCTATGCGCTTTTGGACTTTGCCCGTGAGAGGAACATTCATATTATATCAAACGAAATCTTTGCTGGGTCAGTCCACGGAGAAGAAGGAGAGTTTGTTAGCATGGCTGAAATAGTTGACACGGAAGAGAATATCGACAGGGAAAGAGTTCATATCGTGTATGACCTTTCGAAAGACTTGTCTTTCCGGGGGCTTAGATCCGCTGCTATCTACTCGTTCAACGAGAGTGTTTTATCCGCTTCAAGAAAGCTCACGACGCTCTCACCTGTCTCATCTCCAACCCAACATTTGCTGATATCCGCAATCTCCAATCCAAAAAATGTTCAGAGATTTGTGAAAACCAACAGGCAGAGATTGCAGAGTATCTACACGGAGCTCGTGGAGGGGTTGAAAGAGTTAGGGATCGAGTGCACAAGAAGCAATGGAGGGTTCTACTGTTGGGCTGATATGCGAGGATTGATTTCATCTTACAGCGAAAAAGGCGAGATTGAGCTGTGGAACAAGCTCTTGAACATTGGCAAGATCAATGTCATACCAGGATCTTGTTGTCACTGTATCGAACCAGGATGGTTCCGTATCTGTTTCAGTAATTTGTCTGAGAGAGATGTGCCAGTAGTTATGAACCGCATTAGAAAAGTTTGTGAAACATGTAAATCTCAAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

557

Amino Acids

61.02

Weight (kDa)

6.69

Isoelectric Point (pI)

53.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 171 - 546 1e-77 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 959
AccII CGCG 1 cut(s) 951
AciI CCGC 9 cut(s) 72, 306, 875, 949, 951, 1209, 1245, 1308, 1633
AclWI GGATC 7 cut(s) 281, 294, 467, 695, 1200, 1418, 1555
AcsI RAATTY 2 cut(s) 55, 373
AcuI CTGAAG 2 cut(s) 167, 693
AfaI GTAC 3 cut(s) 10, 123, 417
AfiI CCNNNNNNNGG 5 cut(s) 93, 113, 275, 629, 1038
AflIII ACRYGT 2 cut(s) 856, 1655
AgsI TTSAA 6 cut(s) 886, 975, 1228, 1251, 1399, 1519
AjnI CCWGG 2 cut(s) 1543, 1570
AjuI GAANNNNNNNTTGG 2 cut(s) 787, 819
Alw21I GWGCWC 3 cut(s) 35, 1386, 1421
Alw26I GTCTC 1 cut(s) 1282
Alw44I GTGCAC 1 cut(s) 1417
AlwI GGATC 7 cut(s) 281, 294, 467, 695, 1200, 1418, 1555
ApaLI GTGCAC 1 cut(s) 1417
ApeKI GCWGC 1 cut(s) 1211
ApoI RAATTY 2 cut(s) 55, 373
ArsI GACNNNNNNTTYG 2 cut(s) 1172, 1204
Asp700I GAANNNNTTC 1 cut(s) 1155
AspLEI GCGC 1 cut(s) 1018
AsuC2I CCSGG 5 cut(s) 88, 295, 497, 810, 1196
AsuHPI GGTGA 3 cut(s) 245, 703, 1263
AsuII TTCGAA 1 cut(s) 1178
BaeGI GKGCMC 1 cut(s) 1421
BamHI GGATCC 1 cut(s) 286
BanII GRGCYC 2 cut(s) 35, 1386
BauI CACGAG 1 cut(s) 1385
Bbv12I GWGCWC 3 cut(s) 35, 1386, 1421
BbvCI CCTCAGC 1 cut(s) 247
BbvI GCAGC 1 cut(s) 1198
BccI CCATC 5 cut(s) 581, 623, 712, 889, 1569
BcgI CGANNNNNNTGC 4 cut(s) 883, 917, 1052, 1086
BciT130I CCWGG 2 cut(s) 1545, 1572
BcnI CCSGG 5 cut(s) 88, 295, 497, 810, 1196
BcoDI GTCTC 1 cut(s) 1282
BisI GCNGC 2 cut(s) 952, 1212
BlpI GCTNAGC 1 cut(s) 763
BlsI GCNGC 2 cut(s) 953, 1213
Bme1390I CCNGG 7 cut(s) 88, 295, 497, 810, 1196, 1545, 1572
BmiI GGNNCC 3 cut(s) 26, 288, 1580
BmrFI CCNGG 7 cut(s) 88, 295, 497, 810, 1196, 1545, 1572
BmsI GCATC 1 cut(s) 740
BplI GAGNNNNNCTC 2 cut(s) 993, 1025
Bpu10I CCTNAGC 1 cut(s) 247
Bpu1102I GCTNAGC 1 cut(s) 763
Bpu14I TTCGAA 1 cut(s) 1178
BpuMI CCSGG 5 cut(s) 88, 295, 497, 810, 1196
BsaJI CCNNGG 4 cut(s) 949, 981, 1084, 1195
BsaWI WCCGGW 5 cut(s) 21, 59, 230, 283, 377
BsaXI ACNNNNNCTCC 2 cut(s) 1089, 1119
Bsc4I CCNNNNNNNGG 5 cut(s) 93, 113, 275, 629, 1038
Bse1I ACTGG 2 cut(s) 334, 1619
Bse3DI GCAATG 2 cut(s) 606, 1435
BseBI CCWGG 2 cut(s) 1545, 1572
BseDI CCNNGG 4 cut(s) 949, 981, 1084, 1195
BseGI GGATG 5 cut(s) 96, 475, 553, 829, 1580
BseLI CCNNNNNNNGG 5 cut(s) 93, 113, 275, 629, 1038
BseMI GCAATG 2 cut(s) 606, 1435
BseMII CTCAG 3 cut(s) 261, 986, 1596
BseNI ACTGG 2 cut(s) 334, 1619
BseRI GAGGAG 2 cut(s) 235, 350
BseSI GKGCMC 1 cut(s) 1421
BseXI GCAGC 1 cut(s) 1198
BseYI CCCAGC 1 cut(s) 1073
Bsh1236I CGCG 1 cut(s) 951
BsiHKAI GWGCWC 3 cut(s) 35, 1386, 1421
BslI CCNNNNNNNGG 5 cut(s) 93, 113, 275, 629, 1038
BsmAI GTCTC 1 cut(s) 1282
BsmI GAATGC 1 cut(s) 914
Bsp119I TTCGAA 1 cut(s) 1178
Bsp1286I GDGCHC 3 cut(s) 35, 1386, 1421
Bsp143I GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
Bsp1720I GCTNAGC 1 cut(s) 763
Bsp19I CCATGG 1 cut(s) 981
BspACI CCGC 9 cut(s) 72, 306, 875, 949, 951, 1209, 1245, 1308, 1633
BspCNI CTCAG 3 cut(s) 260, 987, 1597
BspFNI CGCG 1 cut(s) 951
BspLI GGNNCC 3 cut(s) 26, 288, 1580
BspPI GGATC 7 cut(s) 281, 294, 467, 695, 1200, 1418, 1555
BspT104I TTCGAA 1 cut(s) 1178
BsrDI GCAATG 2 cut(s) 606, 1435
BsrI ACTGG 2 cut(s) 334, 1619
BssECI CCNNGG 4 cut(s) 949, 981, 1084, 1195
BssMI GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
BssSI CACGAG 1 cut(s) 1385
BssT1I CCWWGG 1 cut(s) 981
Bst2BI CACGAG 1 cut(s) 1385
Bst2UI CCWGG 2 cut(s) 1545, 1572
Bst4CI ACNGT 3 cut(s) 711, 1445, 1562
Bst6I CTCTTC 1 cut(s) 1128
BstAPI GCANNNNNTGC 2 cut(s) 737, 1363
BstBI TTCGAA 1 cut(s) 1178
BstDEI CTNAG 7 cut(s) 145, 247, 763, 927, 995, 1202, 1605
BstDSI CCRYGG 3 cut(s) 949, 981, 1084
BstEII GGTNACC 1 cut(s) 491
BstF5I GGATG 5 cut(s) 96, 475, 553, 829, 1580
BstFNI CGCG 1 cut(s) 951
BstHHI GCGC 1 cut(s) 1018
BstKTI GATC 8 cut(s) 289, 475, 703, 910, 1208, 1413, 1533, 1550
BstMAI GTCTC 1 cut(s) 1282
BstMBI GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
BstMWI GCNNNNNNNGC 6 cut(s) 737, 996, 1264, 1363, 1425, 1488
BstNI CCWGG 2 cut(s) 1545, 1572
BstNSI RCATGY 1 cut(s) 1659
BstPI GGTNACC 1 cut(s) 491
BstSCI CCNGG 7 cut(s) 86, 293, 495, 808, 1194, 1543, 1570
BstSLI GKGCMC 1 cut(s) 1421
BstUI CGCG 1 cut(s) 951
BstV1I GCAGC 1 cut(s) 1198
BstX2I RGATCY 3 cut(s) 286, 1205, 1547
BstYI RGATCY 3 cut(s) 286, 1205, 1547
BtgI CCRYGG 3 cut(s) 949, 981, 1084
BtsCI GGATG 5 cut(s) 96, 475, 553, 829, 1580
BtsIMutI CAGTG 1 cut(s) 1558
CfoI GCGC 1 cut(s) 1018
Cfr42I CCGCGG 1 cut(s) 952
CseI GACGC 1 cut(s) 1273
Csp6I GTAC 3 cut(s) 9, 122, 416
CviAII CATG 4 cut(s) 864, 982, 1111, 1656
CviQI GTAC 3 cut(s) 9, 122, 416
DdeI CTNAG 7 cut(s) 145, 247, 763, 927, 995, 1202, 1605
DpnI GATC 8 cut(s) 288, 474, 702, 909, 1207, 1412, 1532, 1549
DpnII GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
EciI GGCGGA 1 cut(s) 87
Ecl136II GAGCTC 2 cut(s) 33, 1384
Eco130I CCWWGG 1 cut(s) 981
Eco24I GRGCYC 2 cut(s) 35, 1386
Eco32I GATATC 1 cut(s) 1305
Eco53kI GAGCTC 2 cut(s) 33, 1384
Eco57I CTGAAG 2 cut(s) 167, 693
Eco91I GGTNACC 1 cut(s) 491
EcoICRI GAGCTC 2 cut(s) 33, 1384
EcoO65I GGTNACC 1 cut(s) 491
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 2 cut(s) 1543, 1570
EcoRV GATATC 1 cut(s) 1305
EcoT14I CCWWGG 1 cut(s) 981
EcoT38I GRGCYC 2 cut(s) 35, 1386
ErhI CCWWGG 1 cut(s) 981
FaeI CATG 4 cut(s) 867, 985, 1114, 1659
FatI CATG 4 cut(s) 863, 981, 1110, 1655
Fnu4HI GCNGC 2 cut(s) 952, 1212
FokI GGATG 5 cut(s) 103, 482, 560, 836, 1587
FriOI GRGCYC 2 cut(s) 35, 1386
Fsp4HI GCNGC 2 cut(s) 952, 1212
GlaI GCGC 1 cut(s) 1017
GluI GCNGC 2 cut(s) 952, 1212
GsaI CCCAGC 1 cut(s) 1077
HgaI GACGC 1 cut(s) 1273
HhaI GCGC 1 cut(s) 1018
Hin1II CATG 4 cut(s) 867, 985, 1114, 1659
Hin6I GCGC 1 cut(s) 1016
HinP1I GCGC 1 cut(s) 1016
HincII GTYRAC 3 cut(s) 720, 847, 1126
HindII GTYRAC 3 cut(s) 720, 847, 1126
HpaI GTTAAC 1 cut(s) 720
HphI GGTGA 3 cut(s) 245, 703, 1263
Hpy166II GTNNAC 9 cut(s) 236, 281, 335, 416, 720, 847, 1084, 1126, 1419
Hpy188I TCNGA 7 cut(s) 43, 49, 223, 586, 628, 1335, 1606
Hpy188III TCNNGA 5 cut(s) 774, 964, 1251, 1261, 1516
Hpy8I GTNNAC 9 cut(s) 236, 281, 335, 416, 720, 847, 1084, 1126, 1419
Hpy99I CGWCG 3 cut(s) 71, 218, 1267
HpyAV CCTTC 5 cut(s) 633, 766, 793, 981, 1088
HpyCH4III ACNGT 3 cut(s) 711, 1445, 1562
HpyCH4IV ACGT 3 cut(s) 66, 447, 858
HpyCH4V TGCA 3 cut(s) 731, 1366, 1419
HpyF10VI GCNNNNNNNGC 6 cut(s) 737, 996, 1264, 1363, 1425, 1488
HpyF3I CTNAG 7 cut(s) 145, 247, 763, 927, 995, 1202, 1605
HpySE526I ACGT 3 cut(s) 66, 447, 858
Hsp92II CATG 4 cut(s) 867, 985, 1114, 1659
HspAI GCGC 1 cut(s) 1016
KspAI GTTAAC 1 cut(s) 720
KspI CCGCGG 1 cut(s) 952
Kzo9I GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
LmnI GCTCC 6 cut(s) 24, 30, 38, 322, 383, 1381
Lsp1109I GCAGC 1 cut(s) 1198
LweI GCATC 1 cut(s) 740
MaeII ACGT 3 cut(s) 66, 447, 858
MaeIII GTNAC 6 cut(s) 62, 108, 297, 491, 691, 1556
MalI GATC 8 cut(s) 288, 474, 702, 909, 1207, 1412, 1532, 1549
MboI GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
MboII GAAGA 9 cut(s) 163, 173, 246, 249, 252, 255, 426, 1103, 1145
MflI RGATCY 3 cut(s) 286, 1205, 1547
MhlI GDGCHC 3 cut(s) 35, 1386, 1421
MmeI TCCRAC 3 cut(s) 535, 818, 1310
MroXI GAANNNNTTC 1 cut(s) 1155
MseI TTAA 3 cut(s) 408, 719, 828
MslI CAYNNNNRTG 1 cut(s) 89
MspA1I CMGCKG 3 cut(s) 308, 951, 1211
MspR9I CCNGG 7 cut(s) 88, 295, 497, 810, 1196, 1545, 1572
Mva1269I GAATGC 1 cut(s) 914
MvaI CCWGG 2 cut(s) 1545, 1572
MvnI CGCG 1 cut(s) 951
MwoI GCNNNNNNNGC 6 cut(s) 737, 996, 1264, 1363, 1425, 1488
NciI CCSGG 5 cut(s) 88, 295, 497, 810, 1196
NcoI CCATGG 1 cut(s) 981
NdeII GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
NlaIII CATG 4 cut(s) 867, 985, 1114, 1659
NlaIV GGNNCC 3 cut(s) 26, 288, 1580
NmeAIII GCCGAG 1 cut(s) 393
NmuCI GTSAC 3 cut(s) 297, 691, 1556
NspI RCATGY 1 cut(s) 1659
NspV TTCGAA 1 cut(s) 1178
PciI ACATGT 1 cut(s) 1655
PctI GAATGC 1 cut(s) 914
PdmI GAANNNNTTC 1 cut(s) 1155
PfoI TCCNGGA 1 cut(s) 808
PkrI GCNGC 2 cut(s) 953, 1213
PscI ACATGT 1 cut(s) 1655
PsiI TTATAA 1 cut(s) 959
Psp124BI GAGCTC 2 cut(s) 35, 1386
Psp6I CCWGG 2 cut(s) 1543, 1570
PspEI GGTNACC 1 cut(s) 491
PspFI CCCAGC 1 cut(s) 1073
PspGI CCWGG 2 cut(s) 1543, 1570
PspN4I GGNNCC 3 cut(s) 26, 288, 1580
PsuI RGATCY 3 cut(s) 286, 1205, 1547
RsaI GTAC 3 cut(s) 10, 123, 417
RsaNI GTAC 3 cut(s) 9, 122, 416
RseI CAYNNNNRTG 1 cut(s) 89
SacI GAGCTC 2 cut(s) 35, 1386
SacII CCGCGG 1 cut(s) 952
SaqAI TTAA 3 cut(s) 408, 719, 828
SatI GCNGC 2 cut(s) 952, 1212
Sau3AI GATC 8 cut(s) 286, 472, 700, 907, 1205, 1410, 1530, 1547
ScrFI CCNGG 7 cut(s) 88, 295, 497, 810, 1196, 1545, 1572
SduI GDGCHC 3 cut(s) 35, 1386, 1421
SfaNI GCATC 1 cut(s) 740
Sfr303I CCGCGG 1 cut(s) 952
SfuI TTCGAA 1 cut(s) 1178
SgrBI CCGCGG 1 cut(s) 952
SmiMI CAYNNNNRTG 1 cut(s) 89
SsiI CCGC 9 cut(s) 72, 306, 875, 949, 951, 1209, 1245, 1308, 1633
SstI GAGCTC 2 cut(s) 35, 1386
StyD4I CCNGG 7 cut(s) 86, 293, 495, 808, 1194, 1543, 1570
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 3 cut(s) 711, 1445, 1562
TaiI ACGT 3 cut(s) 69, 450, 861
TaqI TCGA 8 cut(s) 465, 893, 910, 965, 1143, 1178, 1413, 1566
TatI WGTACW 1 cut(s) 415
TauI GCSGC 1 cut(s) 954
Tru1I TTAA 3 cut(s) 408, 719, 828
Tru9I TTAA 3 cut(s) 408, 719, 828
TscAI CASTG 1 cut(s) 1565
TseFI GTSAC 3 cut(s) 297, 691, 1556
TseI GCWGC 1 cut(s) 1211
Tsp45I GTSAC 3 cut(s) 297, 691, 1556
TspDTI ATGAA 4 cut(s) 1037, 1148, 1461, 1643
TspGWI ACGGA 9 cut(s) 96, 335, 465, 516, 789, 1101, 1145, 1394, 1571
TspRI CASTG 1 cut(s) 1565
VneI GTGCAC 1 cut(s) 1417
XapI RAATTY 2 cut(s) 55, 373
XceI RCATGY 1 cut(s) 1659
XmnI GAANNNNTTC 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.