RLG00000013085

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0008719
Physical Location & Seq
Forward (+)
26016069 .. 26020952
4884 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013085

Sequence Viewer

Length: 858 bp
ATGGGTGAGAGGATACATCTAGATCGTAAAGACCTTAGCAGGAGGAGACCCGGTCTTCGGACTTTACCCGTCAAGCAATGCGATCCAAGTCAGCAGGGGCCCACTGCATTTGTTTTGTTGTTGCAAGCAGGCTCGGACCCATGTGCTCACATCGCCTTTTCCAGTCAAAAGGTGTATCCTAGTGGGGTTGCCTTTAACAAAAGGACAAGAGAAACTAGTGCGGATTCCCTCATGTTTCTCGCTCTTCAGTTTTTGCTCTTTGCTTTCAGCGAAGAGTATTCTGCGGACCAAGGTCCACCTGCACCACCAAGCCGAAATTCGGCGAGTGAACGACACGCGCGGTTAAGTAACCGCGGCTGCCGGCTGAATGCAAAGGATGCAGTACTGGGTGGTGAGGAGCTTGGGATTAGTGGGATTGCTTGTTACCAGCCTTCTGATGGTTTAATGGAGCTGAAACTGGCTGTGGCAGGATTCATGTCTAAAGCCGTTGGAAATTCAGTTACCTACAACCCCTCACAAATTGTATTGACAGCTGGTGCAACCCCTGCAATTGAGATTCTAAGCTTCTGCGTAGCAGACAGTGGAAACGCATTTATTGTTCTGGCACCATATTACCCTAGTAATCTTGGGAAGATGAAGACACCCATGAAGTTTATAGCTGCGTTGATGTGTATTTCGTTACACAGGCTGCTAGAGTCTTTGAAAGTCAATTGCCAGAGTCCATCTTGGAAGGTGATGGGCGACTTGCATTTCAAAAGCTTGCAACTACAACACTTGAAATCTAATGCCGAAGATAGAAGGGAACAGAGAGTTTGGTCAGGCAAGTTGGAGGCTAGTGTATACACCAGAGATCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

286

Amino Acids

31.19

Weight (kDa)

9.17

Isoelectric Point (pI)

51.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 137 - 207 1.3e-08 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 307
Acc36I ACCTGC 1 cut(s) 307
AccB1I GGYRCC 1 cut(s) 604
AccI GTMKAC 1 cut(s) 840
AccII CGCG 3 cut(s) 338, 340, 354
AciI CCGC 5 cut(s) 221, 284, 340, 352, 354
AclWI GGATC 2 cut(s) 77, 845
AcsI RAATTY 2 cut(s) 316, 493
AcuI CTGAAG 1 cut(s) 230
AfaI GTAC 1 cut(s) 384
AfiI CCNNNNNNNGG 3 cut(s) 57, 319, 437
AgsI TTSAA 3 cut(s) 703, 754, 778
AhlI ACTAGT 1 cut(s) 215
AjuI GAANNNNNNNTTGG 2 cut(s) 301, 333
AluBI AGCT 6 cut(s) 400, 451, 533, 564, 659, 759
AluI AGCT 6 cut(s) 400, 451, 533, 564, 659, 759
Alw21I GWGCWC 1 cut(s) 148
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 2 cut(s) 77, 845
AoxI GGCC 1 cut(s) 98
ApaI GGGCCC 1 cut(s) 102
ApeKI GCWGC 3 cut(s) 357, 659, 688
ApoI RAATTY 2 cut(s) 316, 493
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AspLEI GCGC 1 cut(s) 340
AspS9I GGNCC 5 cut(s) 98, 99, 136, 286, 293
AsuC2I CCSGG 1 cut(s) 51
AsuHPI GGTGA 3 cut(s) 17, 404, 745
AvaII GGWCC 3 cut(s) 136, 286, 293
BaeGI GKGCMC 1 cut(s) 102
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 1 cut(s) 102
BbsI GAAGAC 2 cut(s) 47, 644
Bbv12I GWGCWC 1 cut(s) 148
BbvI GCAGC 3 cut(s) 344, 646, 675
BccI CCATC 3 cut(s) 431, 730, 730
BceAI ACGGC 1 cut(s) 470
BciVI GTATCC 2 cut(s) 6, 186
BcnI CCSGG 1 cut(s) 51
BcoDI GTCTC 1 cut(s) 40
BcuI ACTAGT 1 cut(s) 215
BfaI CTAG 7 cut(s) 20, 180, 216, 618, 692, 834, 856
BfuAI ACCTGC 1 cut(s) 307
BfuI GTATCC 2 cut(s) 6, 186
BisI GCNGC 4 cut(s) 355, 358, 660, 689
BlsI GCNGC 4 cut(s) 356, 359, 661, 690
BmcAI AGTACT 1 cut(s) 384
Bme1390I CCNGG 1 cut(s) 51
Bme18I GGWCC 3 cut(s) 136, 286, 293
BmgT120I GGNCC 5 cut(s) 98, 99, 136, 286, 293
BmiI GGNNCC 4 cut(s) 99, 100, 138, 606
BmrFI CCNGG 1 cut(s) 51
BmrI ACTGGG 1 cut(s) 395
BmsI GCATC 1 cut(s) 367
BmuI ACTGGG 1 cut(s) 395
BoxI GACNNNNGTC 1 cut(s) 291
BpiI GAAGAC 2 cut(s) 47, 644
Bpu10I CCTNAGC 1 cut(s) 35
BpuMI CCSGG 1 cut(s) 51
BsaI GGTCTC 1 cut(s) 40
BsaJI CCNNGG 2 cut(s) 289, 352
BsaXI ACNNNNNCTCC 2 cut(s) 37, 67
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 319, 437
Bse118I RCCGGY 1 cut(s) 360
Bse1I ACTGG 3 cut(s) 162, 390, 462
Bse3DI GCAATG 1 cut(s) 83
BseDI CCNNGG 2 cut(s) 289, 352
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 3 cut(s) 57, 319, 437
BseMI GCAATG 1 cut(s) 83
BseNI ACTGG 3 cut(s) 162, 390, 462
BseRI GAGGAG 2 cut(s) 58, 410
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 3 cut(s) 344, 646, 675
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 3 cut(s) 338, 340, 354
BshFI GGCC 1 cut(s) 100
BshNI GGYRCC 1 cut(s) 604
BsiHKAI GWGCWC 1 cut(s) 148
BsiSI CCGG 2 cut(s) 51, 361
BslI CCNNNNNNNGG 3 cut(s) 57, 319, 437
BsmAI GTCTC 1 cut(s) 40
BsmI GAATGC 1 cut(s) 373
BsnI GGCC 1 cut(s) 100
Bso31I GGTCTC 1 cut(s) 40
Bsp120I GGGCCC 1 cut(s) 98
Bsp1286I GDGCHC 2 cut(s) 102, 148
Bsp143I GATC 3 cut(s) 22, 82, 850
BspACI CCGC 5 cut(s) 221, 284, 340, 352, 354
BspANI GGCC 1 cut(s) 100
BspFNI CGCG 3 cut(s) 338, 340, 354
BspLI GGNNCC 4 cut(s) 99, 100, 138, 606
BspMI ACCTGC 1 cut(s) 307
BspPI GGATC 2 cut(s) 77, 845
BspQI GCTCTTC 1 cut(s) 249
BspT107I GGYRCC 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 40
BsrDI GCAATG 1 cut(s) 83
BsrFI RCCGGY 1 cut(s) 360
BsrI ACTGG 3 cut(s) 162, 390, 462
BssAI RCCGGY 1 cut(s) 360
BssECI CCNNGG 2 cut(s) 289, 352
BssMI GATC 3 cut(s) 22, 82, 850
BssNAI GTATAC 1 cut(s) 841
BssT1I CCWWGG 1 cut(s) 289
Bst1107I GTATAC 1 cut(s) 841
Bst4CI ACNGT 1 cut(s) 581
Bst6I CTCTTC 2 cut(s) 249, 267
BstAPI GCANNNNNTGC 2 cut(s) 377, 545
BstC8I GCNNGC 4 cut(s) 126, 130, 362, 761
BstDEI CTNAG 2 cut(s) 35, 560
BstDSI CCRYGG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 3 cut(s) 338, 340, 354
BstHHI GCGC 1 cut(s) 340
BstKTI GATC 3 cut(s) 25, 85, 853
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 3 cut(s) 22, 82, 850
BstMWI GCNNNNNNNGC 3 cut(s) 152, 377, 545
BstPAI GACNNNNGTC 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 49
BstSLI GKGCMC 1 cut(s) 102
BstUI CGCG 3 cut(s) 338, 340, 354
BstV1I GCAGC 3 cut(s) 344, 646, 675
BstV2I GAAGAC 2 cut(s) 47, 644
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
BstZ17I GTATAC 1 cut(s) 841
BsuI GTATCC 2 cut(s) 6, 186
BsuRI GGCC 1 cut(s) 100
BtgI CCRYGG 1 cut(s) 352
BtgZI GCGATG 1 cut(s) 136
BtsCI GGATG 1 cut(s) 382
BtsI GCAGTG 1 cut(s) 102
BtsIMutI CAGTG 2 cut(s) 102, 586
BveI ACCTGC 1 cut(s) 307
Cac8I GCNNGC 4 cut(s) 126, 130, 362, 761
CfoI GCGC 1 cut(s) 340
Cfr10I RCCGGY 1 cut(s) 360
Cfr13I GGNCC 5 cut(s) 98, 99, 136, 286, 293
Cfr42I CCGCGG 1 cut(s) 355
Csp6I GTAC 1 cut(s) 383
CspCI CAANNNNNGTGG 2 cut(s) 91, 126
CviAII CATG 4 cut(s) 141, 232, 475, 646
CviQI GTAC 1 cut(s) 383
DdeI CTNAG 2 cut(s) 35, 560
DpnI GATC 3 cut(s) 24, 84, 852
DpnII GATC 3 cut(s) 22, 82, 850
Eam1104I CTCTTC 2 cut(s) 249, 267
EarI CTCTTC 2 cut(s) 249, 267
Eco130I CCWWGG 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 102
Eco31I GGTCTC 1 cut(s) 40
Eco47I GGWCC 3 cut(s) 136, 286, 293
Eco57I CTGAAG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 98
EcoT14I CCWWGG 1 cut(s) 289
EcoT38I GRGCYC 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 289
FaeI CATG 4 cut(s) 144, 235, 478, 649
FaiI YATR 7 cut(s) 142, 233, 476, 610, 647, 656, 841
FatI CATG 4 cut(s) 140, 231, 474, 645
FblI GTMKAC 1 cut(s) 840
Fnu4HI GCNGC 4 cut(s) 355, 358, 660, 689
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 102
Fsp4HI GCNGC 4 cut(s) 355, 358, 660, 689
FspBI CTAG 7 cut(s) 20, 180, 216, 618, 692, 834, 856
GlaI GCGC 1 cut(s) 339
GluI GCNGC 4 cut(s) 355, 358, 660, 689
HaeIII GGCC 1 cut(s) 100
HapII CCGG 2 cut(s) 51, 361
HhaI GCGC 1 cut(s) 340
Hin1II CATG 4 cut(s) 144, 235, 478, 649
Hin6I GCGC 1 cut(s) 338
HinP1I GCGC 1 cut(s) 338
HindIII AAGCTT 2 cut(s) 562, 757
HinfI GANTC 5 cut(s) 224, 471, 556, 695, 718
HpaII CCGG 2 cut(s) 51, 361
HphI GGTGA 3 cut(s) 17, 404, 745
Hpy166II GTNNAC 3 cut(s) 296, 329, 841
Hpy188I TCNGA 3 cut(s) 60, 136, 436
Hpy188III TCNNGA 1 cut(s) 20
Hpy8I GTNNAC 3 cut(s) 296, 329, 841
HpyAV CCTTC 3 cut(s) 441, 724, 792
HpyCH4III ACNGT 1 cut(s) 581
HpyCH4V TGCA 9 cut(s) 107, 124, 302, 371, 380, 539, 548, 748, 763
HpyF10VI GCNNNNNNNGC 3 cut(s) 152, 377, 545
HpyF3I CTNAG 2 cut(s) 35, 560
Hsp92II CATG 4 cut(s) 144, 235, 478, 649
HspAI GCGC 1 cut(s) 338
KroI GCCGGC 1 cut(s) 360
KroNI GCCGGC 1 cut(s) 362
KspI CCGCGG 1 cut(s) 355
Kzo9I GATC 3 cut(s) 22, 82, 850
LguI GCTCTTC 1 cut(s) 249
LmnI GCTCC 2 cut(s) 397, 448
Lsp1109I GCAGC 3 cut(s) 344, 646, 675
LweI GCATC 1 cut(s) 367
MaeI CTAG 7 cut(s) 20, 180, 216, 618, 692, 834, 856
MaeIII GTNAC 4 cut(s) 347, 422, 499, 678
MalI GATC 3 cut(s) 24, 84, 852
MboI GATC 3 cut(s) 22, 82, 850
MboII GAAGA 6 cut(s) 47, 236, 284, 643, 649, 803
MfeI CAATTG 2 cut(s) 549, 709
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 2 cut(s) 102, 148
MluCI AATT 5 cut(s) 316, 493, 519, 549, 709
MlyI GAGTC 2 cut(s) 704, 727
MmeI TCCRAC 2 cut(s) 469, 807
MnlI CCTC 6 cut(s) 3, 36, 239, 388, 523, 823
MroNI GCCGGC 1 cut(s) 360
MseI TTAA 3 cut(s) 195, 344, 443
MspA1I CMGCKG 2 cut(s) 354, 533
MspI CCGG 2 cut(s) 51, 361
MspR9I CCNGG 1 cut(s) 51
MunI CAATTG 2 cut(s) 549, 709
Mva1269I GAATGC 1 cut(s) 373
MvnI CGCG 3 cut(s) 338, 340, 354
MwoI GCNNNNNNNGC 3 cut(s) 152, 377, 545
NaeI GCCGGC 1 cut(s) 362
NciI CCSGG 1 cut(s) 51
NdeII GATC 3 cut(s) 22, 82, 850
NgoMIV GCCGGC 1 cut(s) 360
NlaIII CATG 4 cut(s) 144, 235, 478, 649
NlaIV GGNNCC 4 cut(s) 99, 100, 138, 606
PaqCI CACCTGC 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 249
PctI GAATGC 1 cut(s) 373
PdiI GCCGGC 1 cut(s) 362
PfeI GAWTC 3 cut(s) 224, 471, 556
PflFI GACNNNGTC 1 cut(s) 51
PkrI GCNGC 4 cut(s) 356, 359, 661, 690
PleI GAGTC 2 cut(s) 703, 726
PpsI GAGTC 2 cut(s) 703, 726
PshAI GACNNNNGTC 1 cut(s) 291
PspN4I GGNNCC 4 cut(s) 99, 100, 138, 606
PspOMI GGGCCC 1 cut(s) 98
PspPI GGNCC 5 cut(s) 98, 99, 136, 286, 293
PsuI RGATCY 1 cut(s) 850
PsyI GACNNNGTC 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 1 cut(s) 384
RsaNI GTAC 1 cut(s) 383
SacII CCGCGG 1 cut(s) 355
SapI GCTCTTC 1 cut(s) 249
SaqAI TTAA 3 cut(s) 195, 344, 443
SatI GCNGC 4 cut(s) 355, 358, 660, 689
Sau3AI GATC 3 cut(s) 22, 82, 850
Sau96I GGNCC 5 cut(s) 98, 99, 136, 286, 293
ScaI AGTACT 1 cut(s) 384
SchI GAGTC 2 cut(s) 704, 727
ScrFI CCNGG 1 cut(s) 51
SduI GDGCHC 2 cut(s) 102, 148
SfaNI GCATC 1 cut(s) 367
Sfr303I CCGCGG 1 cut(s) 355
SgrBI CCGCGG 1 cut(s) 355
SinI GGWCC 3 cut(s) 136, 286, 293
SpeI ACTAGT 1 cut(s) 215
Sse9I AATT 5 cut(s) 316, 493, 519, 549, 709
SsiI CCGC 5 cut(s) 221, 284, 340, 352, 354
SspMI CTAG 7 cut(s) 20, 180, 216, 618, 692, 834, 856
StyD4I CCNGG 1 cut(s) 49
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 1 cut(s) 581
TasI AATT 5 cut(s) 316, 493, 519, 549, 709
TatI WGTACW 1 cut(s) 382
TauI GCSGC 1 cut(s) 357
TfiI GAWTC 3 cut(s) 224, 471, 556
Tru1I TTAA 3 cut(s) 195, 344, 443
Tru9I TTAA 3 cut(s) 195, 344, 443
TscAI CASTG 2 cut(s) 109, 586
TseI GCWGC 3 cut(s) 357, 659, 688
TspDTI ATGAA 3 cut(s) 463, 650, 662
TspRI CASTG 2 cut(s) 109, 586
Tth111I GACNNNGTC 1 cut(s) 51
VpaK11BI GGWCC 3 cut(s) 136, 286, 293
XapI RAATTY 2 cut(s) 316, 493
XbaI TCTAGA 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 434
XmiI GTMKAC 1 cut(s) 840
XspI CTAG 7 cut(s) 20, 180, 216, 618, 692, 834, 856
ZrmI AGTACT 1 cut(s) 384
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.