Rh4BG116800

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008719
Physical Location & Seq
Reverse (-)
19659716 .. 19661378
1663 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG116800.1

Sequence Viewer

Length: 549 bp
ATGGACCTCCAAGAAGCAGAAGCAGAAGCAGAAGAAGAAGCTCACCCGCACCGCCGACGAGACCTCGAGTCAAAGCTCGGTTTCGATCTATTCTCCGAACGTGAAACTAGGCTCGGCTTTCGCAACGGTGAGCCGCCGTATTATGTCGGGTTGAGGAAGGTGGCGGAGGATCCATACGACGAGTTGGGTAACCCGGATGGGGTTATTCAGCTGGGTTTGGATGAAAACACGTTAGCTTTGGACTTGGTTCGAGAGTGGCTAATGAATGCAAAGGATGCAATACTGGGTGGTGAGGAGCTTGGGATTAGTGGGATTGCTTGTTACCAGCCTTCTGATGGTTTAATGGAGCTGAAACTGGCTGTGGCAGGATTCATGTCTAAAGCCGTTGGAAATTCAGTTACCTACAACCCCTCACAAATTGTATTGACAGCTGGTGTAACCCCTGCAATTGAGATTCTAAGCTTCTGCCTAGCAGACAGTGGAAACACATTTATTGTTCCGGCACCATATTACCCTGGTTTGGACAGAGATGTGAAGTGGCGAACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

182

Amino Acids

20.04

Weight (kDa)

4.44

Isoelectric Point (pI)

45.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 69 - 181 4.8e-14 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 502
AciI CCGC 4 cut(s) 47, 52, 134, 164
AclWI GGATC 2 cut(s) 164, 177
AcsI RAATTY 1 cut(s) 391
AfiI CCNNNNNNNGG 3 cut(s) 199, 335, 520
AflIII ACRYGT 1 cut(s) 228
AhdI GACNNNNNGTC 1 cut(s) 67
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 8 cut(s) 41, 76, 211, 236, 298, 349, 431, 462
AluI AGCT 8 cut(s) 41, 76, 211, 236, 298, 349, 431, 462
Alw26I GTCTC 1 cut(s) 54
AlwI GGATC 2 cut(s) 164, 177
Ama87I CYCGRG 1 cut(s) 65
ApoI RAATTY 1 cut(s) 391
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 194
AsuHPI GGTGA 3 cut(s) 35, 140, 302
AvaI CYCGRG 1 cut(s) 65
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 1 cut(s) 169
BanI GGYRCC 1 cut(s) 502
BccI CCATC 2 cut(s) 191, 329
BceAI ACGGC 2 cut(s) 121, 368
BciT130I CCWGG 1 cut(s) 516
BcnI CCSGG 1 cut(s) 194
BcoDI GTCTC 1 cut(s) 54
BfaI CTAG 2 cut(s) 108, 470
BisI GCNGC 1 cut(s) 134
BlsI GCNGC 1 cut(s) 135
Bme1390I CCNGG 2 cut(s) 194, 516
Bme18I GGWCC 1 cut(s) 4
BmeRI GACNNNNNGTC 1 cut(s) 67
BmeT110I CYCGRG 1 cut(s) 65
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 2 cut(s) 171, 504
BmrFI CCNGG 2 cut(s) 194, 516
BmrI ACTGGG 1 cut(s) 293
BmsI GCATC 1 cut(s) 265
BmuI ACTGGG 1 cut(s) 293
BpuMI CCSGG 1 cut(s) 194
BsaI GGTCTC 1 cut(s) 54
BsaJI CCNNGG 1 cut(s) 514
Bsc4I CCNNNNNNNGG 3 cut(s) 199, 335, 520
Bse1I ACTGG 2 cut(s) 288, 360
BseBI CCWGG 1 cut(s) 516
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 3 cut(s) 202, 226, 280
BseLI CCNNNNNNNGG 3 cut(s) 199, 335, 520
BseNI ACTGG 2 cut(s) 288, 360
BseRI GAGGAG 1 cut(s) 308
BseYI CCCAGC 1 cut(s) 211
BshNI GGYRCC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 65
BsiSI CCGG 2 cut(s) 194, 500
BslI CCNNNNNNNGG 3 cut(s) 199, 335, 520
BsmAI GTCTC 1 cut(s) 54
BsmI GAATGC 1 cut(s) 271
Bso31I GGTCTC 1 cut(s) 54
BsoBI CYCGRG 1 cut(s) 65
Bsp143I GATC 2 cut(s) 85, 169
BspACI CCGC 4 cut(s) 47, 52, 134, 164
BspLI GGNNCC 2 cut(s) 171, 504
BspPI GGATC 2 cut(s) 164, 177
BspT107I GGYRCC 1 cut(s) 502
BspTNI GGTCTC 1 cut(s) 54
BsrI ACTGG 2 cut(s) 288, 360
BssECI CCNNGG 1 cut(s) 514
BssMI GATC 2 cut(s) 85, 169
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 2 cut(s) 128, 479
BstAPI GCANNNNNTGC 1 cut(s) 275
BstDEI CTNAG 1 cut(s) 458
BstEII GGTNACC 1 cut(s) 188
BstF5I GGATG 3 cut(s) 202, 226, 280
BstKTI GATC 2 cut(s) 88, 172
BstMAI GTCTC 1 cut(s) 54
BstMBI GATC 2 cut(s) 85, 169
BstMWI GCNNNNNNNGC 1 cut(s) 275
BstNI CCWGG 1 cut(s) 516
BstPI GGTNACC 1 cut(s) 188
BstSCI CCNGG 2 cut(s) 192, 514
BstX2I RGATCY 1 cut(s) 169
BstYI RGATCY 1 cut(s) 169
BtsCI GGATG 3 cut(s) 202, 226, 280
BtsIMutI CAGTG 1 cut(s) 484
Cfr13I GGNCC 1 cut(s) 4
CviAII CATG 1 cut(s) 373
DdeI CTNAG 1 cut(s) 458
DpnI GATC 2 cut(s) 87, 171
DpnII GATC 2 cut(s) 85, 169
DriI GACNNNNNGTC 1 cut(s) 67
Eam1105I GACNNNNNGTC 1 cut(s) 67
EciI GGCGGA 1 cut(s) 179
Eco31I GGTCTC 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 4
Eco88I CYCGRG 1 cut(s) 65
Eco91I GGTNACC 1 cut(s) 188
EcoO65I GGTNACC 1 cut(s) 188
EcoRII CCWGG 1 cut(s) 514
FaeI CATG 1 cut(s) 376
FaiI YATR 4 cut(s) 144, 175, 374, 508
FatI CATG 1 cut(s) 372
FauI CCCGC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 134
FokI GGATG 3 cut(s) 209, 233, 287
Fsp4HI GCNGC 1 cut(s) 134
FspBI CTAG 2 cut(s) 108, 470
GluI GCNGC 1 cut(s) 134
GsaI CCCAGC 1 cut(s) 215
HapII CCGG 2 cut(s) 194, 500
Hin1II CATG 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 460
HinfI GANTC 3 cut(s) 68, 369, 454
HpaII CCGG 2 cut(s) 194, 500
HphI GGTGA 3 cut(s) 35, 140, 302
Hpy188I TCNGA 2 cut(s) 97, 334
Hpy188III TCNNGA 1 cut(s) 251
Hpy99I CGWCG 2 cut(s) 60, 182
HpyAV CCTTC 2 cut(s) 151, 339
HpyCH4III ACNGT 2 cut(s) 128, 479
HpyCH4IV ACGT 2 cut(s) 100, 230
HpyCH4V TGCA 3 cut(s) 269, 278, 446
HpyF10VI GCNNNNNNNGC 1 cut(s) 275
HpyF3I CTNAG 1 cut(s) 458
HpySE526I ACGT 2 cut(s) 100, 230
Hsp92II CATG 1 cut(s) 376
Kzo9I GATC 2 cut(s) 85, 169
LmnI GCTCC 2 cut(s) 295, 346
LweI GCATC 1 cut(s) 265
MaeI CTAG 2 cut(s) 108, 470
MaeII ACGT 2 cut(s) 100, 230
MaeIII GTNAC 4 cut(s) 188, 320, 397, 436
MalI GATC 2 cut(s) 87, 171
MboI GATC 2 cut(s) 85, 169
MboII GAAGA 2 cut(s) 44, 47
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 1 cut(s) 169
MluCI AATT 3 cut(s) 391, 417, 447
MlyI GAGTC 1 cut(s) 77
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 6 cut(s) 17, 74, 147, 160, 286, 421
MseI TTAA 1 cut(s) 341
MspA1I CMGCKG 2 cut(s) 211, 431
MspI CCGG 2 cut(s) 194, 500
MspR9I CCNGG 2 cut(s) 194, 516
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 1 cut(s) 271
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 1 cut(s) 275
NciI CCSGG 1 cut(s) 194
NdeII GATC 2 cut(s) 85, 169
NlaIII CATG 1 cut(s) 376
NlaIV GGNNCC 2 cut(s) 171, 504
NmeAIII GCCGAG 1 cut(s) 93
PaeR7I CTCGAG 1 cut(s) 65
PctI GAATGC 1 cut(s) 271
PfeI GAWTC 2 cut(s) 369, 454
PkrI GCNGC 1 cut(s) 135
PleI GAGTC 1 cut(s) 76
PpsI GAGTC 1 cut(s) 76
Psp6I CCWGG 1 cut(s) 514
PspEI GGTNACC 1 cut(s) 188
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 2 cut(s) 171, 504
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 65
PsuI RGATCY 1 cut(s) 169
PvuII CAGCTG 2 cut(s) 211, 431
SaqAI TTAA 1 cut(s) 341
SatI GCNGC 1 cut(s) 134
Sau3AI GATC 2 cut(s) 85, 169
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 77
ScrFI CCNGG 2 cut(s) 194, 516
SfaNI GCATC 1 cut(s) 265
Sfr274I CTCGAG 1 cut(s) 65
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 65
SmlI CTYRAG 1 cut(s) 65
SmoI CTYRAG 1 cut(s) 65
Sse9I AATT 3 cut(s) 391, 417, 447
SsiI CCGC 4 cut(s) 47, 52, 134, 164
SspMI CTAG 2 cut(s) 108, 470
StyD4I CCNGG 2 cut(s) 192, 514
TaaI ACNGT 2 cut(s) 128, 479
TaiI ACGT 2 cut(s) 103, 233
TaqI TCGA 3 cut(s) 66, 84, 250
TasI AATT 3 cut(s) 391, 417, 447
TauI GCSGC 1 cut(s) 136
TfiI GAWTC 2 cut(s) 369, 454
Tru1I TTAA 1 cut(s) 341
Tru9I TTAA 1 cut(s) 341
TscAI CASTG 1 cut(s) 484
TspDTI ATGAA 3 cut(s) 237, 278, 361
TspRI CASTG 1 cut(s) 484
VpaK11BI GGWCC 1 cut(s) 4
XapI RAATTY 1 cut(s) 391
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XhoI CTCGAG 1 cut(s) 65
XspI CTAG 2 cut(s) 108, 470
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.