MD00G1072200.v1.1

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008719
Physical Location & Seq
Reverse (-)
14148936 .. 14151505
2570 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1072200.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGACCCGGACCCGGGCCCCCGTCCCAGACACCAACCGCTCCAAAGTCACTGGCGGAGGCGGCGGCAACAGCGCCATGAGAGTTATAGTCCCTCTCAAGGGCGTCGTCCAAGGCAGGGGAGGTCTGCTTTTGGGCTCGGTCATACCGTGCGCCTTGTTTTATTTCCTCCAGCTCTATCTGAGACAACACCACTCGAACCCCAACCCCCCAACCCCAACCTGCCTACGCCTTCGCCGGAATCCGAGTCCGAGCATGCCGGACAGCCGGTGGAACTTCCCGTTCTGCCCCGGAGTGTCTCCCGGACCCTCCTCGCCCCGAGGTGGCCAGTCGCCGTACGACGTTGGGTTGAGGAAGGTGGCGGAAGATCCGTATGACGAGTTGAGTAACCCGAATGGGGTTATTCAATTGGGTTTGGATGAAAACAAGTTATCGTTGGATTTGGTTGGAGATTGGTTCCTGGAGAATGCAAAGGAAGCAATTCTGGGTGGCGGCGAGCTCGGAATTTGTGGGATTGCTTGTTACCAGCCTCTTGATGGATTGATGGAGTTAAAAGTGGCTGTGGCAGGATTCATGTCTCAAGTCATGGGGAAGTCAGTTTTCTTCGACCCCTCCCAAATAGTATTGACAGCTGGTGCAACACCTGCAATTGAGATACTAAGTTTCTGCCTAGCAGACAGCGGAAATGCATTTCTTGTTCCGGCGCCATATTACCCTGGTTTGGACAGAGATGTAAAGTGGCGAACTGGAGGAGAGATTATACCTGTTCCATGCCGTAGTGCTGACAAATTCAACTTGTGCGTGACTGCTCTTGAACGTGCATTCAACCAGGCAAAGAAACGCGGTGTAAAGGTTCGTGGGATTATAATTTCAAATCCTTCAAATCCCGTTGGCAGTTTATATAGTTGTGAATCACTTTACAACCTTCTAGACTTTGCCCGAGAGAAAAACATACATATAATCTCAAATGAAATATTTTCTGGATCCATGCATGGTAGTGAAGAGTTTGTAAGCATGGCAGAAATTGTTGAATCGGAAGATATCGACCAGAACAGAGTTCATATAGTATATGGTCTATCGAAAGACCTTTCTCTTCCAGGTTTTAGGGCCAGCGCTATCTACTCCTTTAACAAGGATGTTCTGGCTGCTGCTAAAAAGTTGACAAGGTTCTCGTCCATTTCCTCCCCATCCCAACAGTTGCTCATCTCTATGCTTTCAGACACCAAATTCATCCGGAAGTTCATTGATACTAGTAGAGAAAGGCTTCGTGGAATGTACCTTAAATTTGTGACAGGTTTGAAGCAATTGGGCATTGAGTGCACCAAGAGCAATGGGGGTTTCAGCTGCTGGGCTGACATGAGCGGGTTAATCCGCTTTTACAGCGAGAAAGGGGAGCTTGAGCTCTGGGATAGATTGTTGAATGTAGCTAAACTAGATGTAACACCCGGATCATCATGTCATTGTATTGAACCCGGATGGTTTCGGTTTTGTTTTACAACGTTGACTGTAAAAGATATTCCTGTTGTTATGGAACGAGTTTGGAAATTTTCCGAAACCAGTAAATCACACTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

524

Amino Acids

57.45

Weight (kDa)

8.52

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 135 - 511 1e-74 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 863
AarI CACCTGC 1 cut(s) 649
Acc36I ACCTGC 2 cut(s) 227, 649
AccB1I GGYRCC 1 cut(s) 700
AccBSI CCGCTC 2 cut(s) 39, 1359
AccII CGCG 1 cut(s) 840
AccIII TCCGGA 1 cut(s) 1230
AclI AACGTT 1 cut(s) 1496
AclWI GGATC 4 cut(s) 359, 975, 988, 1453
AcoI YGGCCR 1 cut(s) 322
AcsI RAATTY 5 cut(s) 501, 785, 1223, 1280, 1541
AcyI GRCGYC 2 cut(s) 102, 701
AfaI GTAC 2 cut(s) 335, 1274
AfeI AGCGCT 1 cut(s) 1111
AfiI CCNNNNNNNGG 9 cut(s) 12, 13, 97, 98, 115, 320, 394, 533, 718
AhlI ACTAGT 1 cut(s) 1247
AjnI CCWGG 4 cut(s) 456, 712, 825, 1093
AluBI AGCT 7 cut(s) 172, 496, 629, 1341, 1393, 1399, 1424
AluI AGCT 7 cut(s) 172, 496, 629, 1341, 1393, 1399, 1424
Alw21I GWGCWC 3 cut(s) 498, 1319, 1401
Alw26I GTCTC 3 cut(s) 175, 300, 579
Alw44I GTGCAC 1 cut(s) 1315
AlwI GGATC 4 cut(s) 359, 975, 988, 1453
AlwNI CAGNNNCTG 1 cut(s) 1344
Ama87I CYCGRG 3 cut(s) 12, 315, 936
Aor13HI TCCGGA 1 cut(s) 1230
Aor51HI AGCGCT 1 cut(s) 1111
AoxI GGCC 3 cut(s) 15, 322, 1104
ApaI GGGCCC 1 cut(s) 19
ApaLI GTGCAC 1 cut(s) 1315
ApeKI GCWGC 3 cut(s) 1142, 1145, 1341
ApoI RAATTY 5 cut(s) 501, 785, 1223, 1280, 1541
Asp700I GAANNNNTTC 2 cut(s) 477, 1260
AspLEI GCGC 4 cut(s) 74, 152, 703, 1112
AspS9I GGNCC 5 cut(s) 9, 15, 16, 302, 1104
AsuC2I CCSGG 7 cut(s) 7, 13, 14, 288, 300, 1443, 1470
AvaI CYCGRG 3 cut(s) 12, 315, 936
AvaII GGWCC 2 cut(s) 9, 302
BaeGI GKGCMC 2 cut(s) 19, 1319
BalI TGGCCA 1 cut(s) 324
BamHI GGATCC 1 cut(s) 980
BanI GGYRCC 1 cut(s) 700
BanII GRGCYC 4 cut(s) 19, 137, 498, 1401
Bbv12I GWGCWC 3 cut(s) 498, 1319, 1401
BbvI GCAGC 3 cut(s) 1129, 1132, 1328
BccI CCATC 4 cut(s) 527, 535, 1192, 1467
BceAI ACGGC 2 cut(s) 316, 756
BciT130I CCWGG 4 cut(s) 458, 714, 827, 1095
BcnI CCSGG 7 cut(s) 7, 13, 14, 288, 300, 1443, 1470
BcoDI GTCTC 3 cut(s) 175, 300, 579
BcuI ACTAGT 1 cut(s) 1247
BfaI CTAG 4 cut(s) 668, 926, 1248, 1430
BfoI RGCGCY 3 cut(s) 75, 704, 1113
BfuAI ACCTGC 2 cut(s) 227, 649
BisI GCNGC 6 cut(s) 61, 64, 490, 1143, 1146, 1342
BlsI GCNGC 6 cut(s) 62, 65, 491, 1144, 1147, 1343
Bme18I GGWCC 2 cut(s) 9, 302
BmeT110I CYCGRG 3 cut(s) 12, 315, 936
BmgT120I GGNCC 5 cut(s) 9, 15, 16, 302, 1104
BmiI GGNNCC 7 cut(s) 11, 17, 18, 304, 455, 702, 982
BpmI CTGGAG 3 cut(s) 152, 479, 765
BpuEI CTTGAG 3 cut(s) 80, 561, 1415
BpuMI CCSGG 7 cut(s) 7, 13, 14, 288, 300, 1443, 1470
BsaHI GRCGYC 2 cut(s) 102, 701
BsaJI CCNNGG 5 cut(s) 12, 109, 286, 316, 712
BsaWI WCCGGW 1 cut(s) 1230
Bsc4I CCNNNNNNNGG 9 cut(s) 12, 13, 97, 98, 115, 320, 394, 533, 718
Bse118I RCCGGY 1 cut(s) 264
Bse1I ACTGG 4 cut(s) 55, 325, 748, 1554
Bse3DI GCAATG 1 cut(s) 1333
BseAI TCCGGA 1 cut(s) 1230
BseBI CCWGG 4 cut(s) 458, 714, 827, 1095
BseDI CCNNGG 5 cut(s) 12, 109, 286, 316, 712
BseGI GGATG 5 cut(s) 421, 1138, 1184, 1227, 1478
BseLI CCNNNNNNNGG 9 cut(s) 12, 13, 97, 98, 115, 320, 394, 533, 718
BseMI GCAATG 1 cut(s) 1333
BseMII CTCAG 1 cut(s) 170
BseNI ACTGG 4 cut(s) 55, 325, 748, 1554
BseRI GAGGAG 2 cut(s) 298, 762
BseSI GKGCMC 2 cut(s) 19, 1319
BseXI GCAGC 3 cut(s) 1129, 1132, 1328
BseYI CCCAGC 1 cut(s) 1344
Bsh1236I CGCG 1 cut(s) 840
BshFI GGCC 3 cut(s) 17, 324, 1106
BshNI GGYRCC 1 cut(s) 700
BsiHKAI GWGCWC 3 cut(s) 498, 1319, 1401
BsiHKCI CYCGRG 3 cut(s) 12, 315, 936
BsiWI CGTACG 1 cut(s) 333
BslFI GGGAC 2 cut(s) 8, 74
BslI CCNNNNNNNGG 9 cut(s) 12, 13, 97, 98, 115, 320, 394, 533, 718
BsmAI GTCTC 3 cut(s) 175, 300, 579
BsmFI GGGAC 2 cut(s) 8, 74
BsmI GAATGC 2 cut(s) 469, 818
BsnI GGCC 3 cut(s) 17, 324, 1106
BsoBI CYCGRG 3 cut(s) 12, 315, 936
Bsp120I GGGCCC 1 cut(s) 15
Bsp1286I GDGCHC 5 cut(s) 19, 137, 498, 1319, 1401
Bsp13I TCCGGA 1 cut(s) 1230
Bsp143I GATC 3 cut(s) 364, 980, 1445
BspANI GGCC 3 cut(s) 17, 324, 1106
BspCNI CTCAG 1 cut(s) 171
BspEI TCCGGA 1 cut(s) 1230
BspFNI CGCG 1 cut(s) 840
BspLI GGNNCC 7 cut(s) 11, 17, 18, 304, 455, 702, 982
BspMI ACCTGC 2 cut(s) 227, 649
BspPI GGATC 4 cut(s) 359, 975, 988, 1453
BspT107I GGYRCC 1 cut(s) 700
BsrBI CCGCTC 2 cut(s) 39, 1359
BsrDI GCAATG 1 cut(s) 1333
BsrFI RCCGGY 1 cut(s) 264
BsrI ACTGG 4 cut(s) 55, 325, 748, 1554
BssAI RCCGGY 1 cut(s) 264
BssECI CCNNGG 5 cut(s) 12, 109, 286, 316, 712
BssMI GATC 3 cut(s) 364, 980, 1445
BssNI GRCGYC 2 cut(s) 102, 701
BssT1I CCWWGG 1 cut(s) 109
Bst2UI CCWGG 4 cut(s) 458, 714, 827, 1095
Bst4CI ACNGT 4 cut(s) 147, 1194, 1504, 1568
Bst6I CTCTTC 2 cut(s) 993, 1095
BstACI GRCGYC 2 cut(s) 102, 701
BstAPI GCANNNNNTGC 2 cut(s) 641, 1314
BstC8I GCNNGC 3 cut(s) 254, 494, 1108
BstDEI CTNAG 2 cut(s) 179, 656
BstF5I GGATG 5 cut(s) 421, 1138, 1184, 1227, 1478
BstFNI CGCG 1 cut(s) 840
BstH2I RGCGCY 3 cut(s) 75, 704, 1113
BstHHI GCGC 4 cut(s) 74, 152, 703, 1112
BstKTI GATC 3 cut(s) 367, 983, 1448
BstMAI GTCTC 3 cut(s) 175, 300, 579
BstMBI GATC 3 cut(s) 364, 980, 1445
BstMWI GCNNNNNNNGC 7 cut(s) 60, 69, 473, 641, 1314, 1323, 1377
BstNI CCWGG 4 cut(s) 458, 714, 827, 1095
BstNSI RCATGY 1 cut(s) 256
BstSLI GKGCMC 2 cut(s) 19, 1319
BstUI CGCG 1 cut(s) 840
BstV1I GCAGC 3 cut(s) 1129, 1132, 1328
BstX2I RGATCY 2 cut(s) 364, 980
BstYI RGATCY 2 cut(s) 364, 980
BsuRI GGCC 3 cut(s) 17, 324, 1106
BtsCI GGATG 5 cut(s) 421, 1138, 1184, 1227, 1478
BtsIMutI CAGTG 2 cut(s) 48, 1564
BveI ACCTGC 2 cut(s) 227, 649
Cac8I GCNNGC 3 cut(s) 254, 494, 1108
CaiI CAGNNNCTG 1 cut(s) 1344
CfoI GCGC 4 cut(s) 74, 152, 703, 1112
Cfr10I RCCGGY 1 cut(s) 264
Cfr13I GGNCC 5 cut(s) 9, 15, 16, 302, 1104
Cfr9I CCCGGG 1 cut(s) 12
CseI GACGC 1 cut(s) 91
Csp6I GTAC 2 cut(s) 334, 1273
CviQI GTAC 2 cut(s) 334, 1273
DdeI CTNAG 2 cut(s) 179, 656
DinI GGCGCC 1 cut(s) 702
DpnI GATC 3 cut(s) 366, 982, 1447
DpnII GATC 3 cut(s) 364, 980, 1445
EaeI YGGCCR 1 cut(s) 322
Eam1104I CTCTTC 2 cut(s) 993, 1095
EarI CTCTTC 2 cut(s) 993, 1095
EciI GGCGGA 2 cut(s) 69, 374
Ecl136II GAGCTC 2 cut(s) 496, 1399
Eco130I CCWWGG 1 cut(s) 109
Eco24I GRGCYC 4 cut(s) 19, 137, 498, 1401
Eco32I GATATC 1 cut(s) 1039
Eco47I GGWCC 2 cut(s) 9, 302
Eco47III AGCGCT 1 cut(s) 1111
Eco53kI GAGCTC 2 cut(s) 496, 1399
Eco88I CYCGRG 3 cut(s) 12, 315, 936
EcoICRI GAGCTC 2 cut(s) 496, 1399
EcoO109I RGGNCCY 1 cut(s) 16
EcoRII CCWGG 4 cut(s) 456, 712, 825, 1093
EcoRV GATATC 1 cut(s) 1039
EcoT14I CCWWGG 1 cut(s) 109
EcoT22I ATGCAT 2 cut(s) 688, 990
EcoT38I GRGCYC 4 cut(s) 19, 137, 498, 1401
EgeI GGCGCC 1 cut(s) 702
EheI GGCGCC 1 cut(s) 702
ErhI CCWWGG 1 cut(s) 109
FalI AAGNNNNNCTT 2 cut(s) 1377, 1409
FaqI GGGAC 2 cut(s) 8, 74
FauI CCCGC 1 cut(s) 1352
Fnu4HI GCNGC 6 cut(s) 61, 64, 490, 1143, 1146, 1342
FokI GGATG 5 cut(s) 428, 1145, 1171, 1214, 1485
FriOI GRGCYC 4 cut(s) 19, 137, 498, 1401
Fsp4HI GCNGC 6 cut(s) 61, 64, 490, 1143, 1146, 1342
FspBI CTAG 4 cut(s) 668, 926, 1248, 1430
GlaI GCGC 4 cut(s) 73, 151, 702, 1111
GluI GCNGC 6 cut(s) 61, 64, 490, 1143, 1146, 1342
GsaI CCCAGC 1 cut(s) 1348
GsuI CTGGAG 3 cut(s) 152, 479, 765
HaeII RGCGCY 3 cut(s) 75, 704, 1113
HaeIII GGCC 3 cut(s) 17, 324, 1106
HgaI GACGC 1 cut(s) 91
HhaI GCGC 4 cut(s) 74, 152, 703, 1112
Hin1I GRCGYC 2 cut(s) 102, 701
Hin6I GCGC 4 cut(s) 72, 150, 701, 1110
HinP1I GCGC 4 cut(s) 72, 150, 701, 1110
HincII GTYRAC 2 cut(s) 1158, 1500
HindII GTYRAC 2 cut(s) 1158, 1500
HinfI GANTC 5 cut(s) 238, 244, 567, 908, 1028
Hpy166II GTNNAC 3 cut(s) 1158, 1317, 1500
Hpy188I TCNGA 7 cut(s) 180, 243, 249, 500, 1033, 1216, 1549
Hpy188III TCNNGA 5 cut(s) 530, 809, 926, 978, 1231
Hpy8I GTNNAC 3 cut(s) 1158, 1317, 1500
Hpy99I CGWCG 2 cut(s) 107, 341
HpyAV CCTTC 4 cut(s) 239, 346, 885, 932
HpyCH4III ACNGT 4 cut(s) 147, 1194, 1504, 1568
HpyCH4IV ACGT 3 cut(s) 339, 814, 1496
HpyCH4V TGCA 7 cut(s) 467, 635, 644, 686, 818, 988, 1317
HpyF10VI GCNNNNNNNGC 7 cut(s) 60, 69, 473, 641, 1314, 1323, 1377
HpyF3I CTNAG 2 cut(s) 179, 656
HpySE526I ACGT 3 cut(s) 339, 814, 1496
Hsp92I GRCGYC 2 cut(s) 102, 701
HspAI GCGC 4 cut(s) 72, 150, 701, 1110
KasI GGCGCC 1 cut(s) 700
Kpn2I TCCGGA 1 cut(s) 1230
Kzo9I GATC 3 cut(s) 364, 980, 1445
LmnI GCTCC 2 cut(s) 44, 1390
Lsp1109I GCAGC 3 cut(s) 1129, 1132, 1328
MaeI CTAG 4 cut(s) 668, 926, 1248, 1430
MaeII ACGT 3 cut(s) 339, 814, 1496
MaeIII GTNAC 6 cut(s) 46, 383, 518, 799, 1285, 1435
MalI GATC 3 cut(s) 366, 982, 1447
MbiI CCGCTC 2 cut(s) 39, 1359
MboI GATC 3 cut(s) 364, 980, 1445
MboII GAAGA 5 cut(s) 374, 592, 1010, 1046, 1082
MfeI CAATTG 3 cut(s) 404, 645, 1301
MflI RGATCY 2 cut(s) 364, 980
MhlI GDGCHC 5 cut(s) 19, 137, 498, 1319, 1401
MlsI TGGCCA 1 cut(s) 324
MluNI TGGCCA 1 cut(s) 324
Mly113I GGCGCC 1 cut(s) 701
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 2 cut(s) 414, 424
Mox20I TGGCCA 1 cut(s) 324
Mph1103I ATGCAT 2 cut(s) 688, 990
MroI TCCGGA 1 cut(s) 1230
MroXI GAANNNNTTC 2 cut(s) 477, 1260
MscI TGGCCA 1 cut(s) 324
MseI TTAA 4 cut(s) 548, 1125, 1278, 1364
MslI CAYNNNNRTG 2 cut(s) 993, 1205
Msp20I TGGCCA 1 cut(s) 324
MspA1I CMGCKG 3 cut(s) 629, 678, 1341
MunI CAATTG 3 cut(s) 404, 645, 1301
Mva1269I GAATGC 2 cut(s) 469, 818
MvaI CCWGG 4 cut(s) 458, 714, 827, 1095
MvnI CGCG 1 cut(s) 840
MwoI GCNNNNNNNGC 7 cut(s) 60, 69, 473, 641, 1314, 1323, 1377
NarI GGCGCC 1 cut(s) 701
NciI CCSGG 7 cut(s) 7, 13, 14, 288, 300, 1443, 1470
NdeII GATC 3 cut(s) 364, 980, 1445
NlaIV GGNNCC 7 cut(s) 11, 17, 18, 304, 455, 702, 982
NmuCI GTSAC 3 cut(s) 46, 799, 1285
NsiI ATGCAT 2 cut(s) 688, 990
NspI RCATGY 1 cut(s) 256
PaeI GCATGC 1 cut(s) 256
PaqCI CACCTGC 1 cut(s) 649
PcsI WCGNNNNNNNCGW 1 cut(s) 143
PctI GAATGC 2 cut(s) 469, 818
PdmI GAANNNNTTC 2 cut(s) 477, 1260
PfeI GAWTC 4 cut(s) 238, 567, 908, 1028
Pfl23II CGTACG 1 cut(s) 333
PfoI TCCNGGA 2 cut(s) 298, 456
PkrI GCNGC 6 cut(s) 62, 65, 491, 1144, 1147, 1343
PleI GAGTC 1 cut(s) 252
PluTI GGCGCC 1 cut(s) 704
PpsI GAGTC 1 cut(s) 252
PsiI TTATAA 1 cut(s) 863
Psp124BI GAGCTC 2 cut(s) 498, 1401
Psp1406I AACGTT 1 cut(s) 1496
Psp6I CCWGG 4 cut(s) 456, 712, 825, 1093
PspFI CCCAGC 1 cut(s) 1344
PspGI CCWGG 4 cut(s) 456, 712, 825, 1093
PspLI CGTACG 1 cut(s) 333
PspN4I GGNNCC 7 cut(s) 11, 17, 18, 304, 455, 702, 982
PspOMI GGGCCC 1 cut(s) 15
PspPI GGNCC 5 cut(s) 9, 15, 16, 302, 1104
PstNI CAGNNNCTG 1 cut(s) 1344
PsuI RGATCY 2 cut(s) 364, 980
PvuII CAGCTG 2 cut(s) 629, 1341
RsaI GTAC 2 cut(s) 335, 1274
RsaNI GTAC 2 cut(s) 334, 1273
RseI CAYNNNNRTG 2 cut(s) 993, 1205
SacI GAGCTC 2 cut(s) 498, 1401
SaqAI TTAA 4 cut(s) 548, 1125, 1278, 1364
SatI GCNGC 6 cut(s) 61, 64, 490, 1143, 1146, 1342
Sau3AI GATC 3 cut(s) 364, 980, 1445
Sau96I GGNCC 5 cut(s) 9, 15, 16, 302, 1104
SchI GAGTC 1 cut(s) 253
SduI GDGCHC 5 cut(s) 19, 137, 498, 1319, 1401
SfoI GGCGCC 1 cut(s) 702
SinI GGWCC 2 cut(s) 9, 302
SmaI CCCGGG 1 cut(s) 14
SmiMI CAYNNNNRTG 2 cut(s) 993, 1205
SmlI CTYRAG 3 cut(s) 95, 576, 1394
SmoI CTYRAG 3 cut(s) 95, 576, 1394
SpeI ACTAGT 1 cut(s) 1247
SphI GCATGC 1 cut(s) 256
SspDI GGCGCC 1 cut(s) 700
SspI AATATT 1 cut(s) 972
SspMI CTAG 4 cut(s) 668, 926, 1248, 1430
SstI GAGCTC 2 cut(s) 498, 1401
StyI CCWWGG 1 cut(s) 109
TaaI ACNGT 4 cut(s) 147, 1194, 1504, 1568
TaiI ACGT 3 cut(s) 342, 817, 1499
TaqI TCGA 4 cut(s) 194, 603, 1041, 1076
TaqII GACCGA 1 cut(s) 127
TauI GCSGC 3 cut(s) 63, 66, 492
TfiI GAWTC 4 cut(s) 238, 567, 908, 1028
Tru1I TTAA 4 cut(s) 548, 1125, 1278, 1364
Tru9I TTAA 4 cut(s) 548, 1125, 1278, 1364
TscAI CASTG 2 cut(s) 55, 1571
TseFI GTSAC 3 cut(s) 46, 799, 1285
TseI GCWGC 3 cut(s) 1142, 1145, 1341
Tsp45I GTSAC 3 cut(s) 46, 799, 1285
TspDTI ATGAA 6 cut(s) 432, 559, 981, 1046, 1216, 1228
TspGWI ACGGA 1 cut(s) 357
TspMI CCCGGG 1 cut(s) 12
TspRI CASTG 2 cut(s) 55, 1571
VneI GTGCAC 1 cut(s) 1315
VpaK11BI GGWCC 2 cut(s) 9, 302
XapI RAATTY 5 cut(s) 501, 785, 1223, 1280, 1541
XbaI TCTAGA 1 cut(s) 925
XceI RCATGY 1 cut(s) 256
XcmI CCANNNNNNNNNTGG 1 cut(s) 530
XmaI CCCGGG 1 cut(s) 12
XmnI GAANNNNTTC 2 cut(s) 477, 1260
XspI CTAG 4 cut(s) 668, 926, 1248, 1430
Zsp2I ATGCAT 2 cut(s) 688, 990
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.