Rw7G016240

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0008719
Physical Location & Seq
Forward (+)
16574486 .. 16577096
2611 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G016240.1

Sequence Viewer

Length: 1611 bp
ATGACCCGGACCCGAGACGAAGACCGGCGACCCACCAGCAGCAGCAGCGGCGGAGGAGGCGCCGTCATGAGAGTTATAGTCCCTCTACAAGGCGTGGTTCAAGGCAGAGGAGGACTCGTTCTCGGCTCCGTCATACCATGCGCGCTCTTCTATTTCCTCCAGCTTTATCTGAAACGTCACCGTTCCAACTCCAACCCGCCGACTCCGCCGCCTTCTCCGGACTCGGACTCGGACCACCACCCGGCCGGGCAGTTGGTGGAAGTTCCGGTTCTGCCCCGGTCTCTGTCGAGGTCCCATCTCTCGCCGAGGAACCCGGGTCCGGTACACGTCTCGGGTCGGGCCAATTCGGTTTTGAAAGGCGGTGAGCCGCCGTATTATGTCGGGTTGAGGAAGGTGGCGGAGGATCCGTACGACGAGTTGGGTAACCCGGATGGGGTTATTCAGCTGGGTTTGGATGAAAACAAGTTAGCTTTGGACTTGGTTCGAGATTGGCTACTGGAGAATGCAAAGGATGCAATACTGGGTGGTGAGGAGCTTGGGATTAGTGGGATTGCTTGTTACCAGCCTTCTGATGGTTTGATGGAGCTGAAACTGGCTGTGGCAGGATTCATGTCTAAGGCCATTGGAAATTCAGTTACGTACAACCCCTCACAAATTGTATTGACAGCTGGTGCAACCCCTGCAATTGAGATTCTAAGCTTCTGCCTAGCAGACAGTGGAAACGCATTTCTCGTTCCGGCACCATATTACCCTGGTTTGGACAGAGATGTGAAGTGGCGAACTGGAGTGGAGATAATACCTGTTCCATGCCGCAGTGCTGACAAATTCAATTTAAGTATAACTGCACTTGATCGAGCATTCAACCAGGCAAAGAAACGTGGCGTAAAAGTTCGTGGGATTATAATTTCAAATCCTTCAAATCCTGGTGGCAGTTTACTTACTCGTGAATCACTTTACAACCTTCTGGACTTTGCCCGAGAGAAGAACATTCATATAATCTCAAATGAATTGTTTGCTGGATCCACGTATGGAAGTGAAGAGTTTGTTAGCATGGCAGAAATCGTGGATTTGGAAGATCTCGACCAGAACAGAGTGCATATAGTATATGGCATATCGAAAGATCTCTCACTTCCAGGTTTCAGGGTGGGTGCCATCTACTCCTTTAACAAGAATGTCTTGACTGCTGCTAAAAAGTTGACAAGGTTCTCTTCTATCTCCGCCCCATCCCAACGGTTGCTTATCTCTATGCTTTCAGACACCAAATTTATGCATAAGTTCATCGAGATTAACAGAGAAAGGCTCCGTGGAATGTATCTTAGATTTGTGACAGGATTGAAGCAATTGGGCATTGAGTGCACAAAGAGCAATGGGGGTTTCTACTGTTGGGCAGACTTGAGTGGGTTAATTCGCTCTTACAGTGAGAAAGGGGAGCTTGAGCTCTGGGATAGGTTGTTGAATGTAGGTAAGCTCAATGTTACTCCTGGATCTTCTTGTCATTGTATTGAACCGGGATGGTTCCGGTTTTGTTTTACGACGTTGACTGAAAAAGATATCCCTGTTGTTATGGAACGAATTCGGAATATTGCCGAAACATGTAAATCACACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

536

Amino Acids

58.78

Weight (kDa)

8.41

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 147 - 525 5.6e-81 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 902
AccB1I GGYRCC 3 cut(s) 59, 739, 1148
AccII CGCG 1 cut(s) 143
AccIII TCCGGA 1 cut(s) 217
AclWI GGATC 5 cut(s) 398, 411, 1014, 1027, 1492
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 4 cut(s) 628, 824, 1262, 1572
AcyI GRCGYC 1 cut(s) 60
AfaI GTAC 3 cut(s) 324, 410, 641
AfiI CCNNNNNNNGG 6 cut(s) 89, 241, 319, 433, 572, 757
AflIII ACRYGT 2 cut(s) 325, 1592
AgsI TTSAA 9 cut(s) 101, 355, 829, 862, 909, 918, 1336, 1456, 1505
AjiI CACGTC 1 cut(s) 328
AjnI CCWGG 5 cut(s) 751, 864, 922, 1132, 1480
AloI GAACNNNNNNTCC 2 cut(s) 102, 134
Alw21I GWGCWC 2 cut(s) 1358, 1440
Alw26I GTCTC 3 cut(s) 9, 285, 334
Alw44I GTGCAC 1 cut(s) 1354
AlwI GGATC 5 cut(s) 398, 411, 1014, 1027, 1492
Ama87I CYCGRG 4 cut(s) 12, 313, 331, 975
Aor13HI TCCGGA 1 cut(s) 217
AoxI GGCC 3 cut(s) 243, 339, 618
ApaLI GTGCAC 1 cut(s) 1354
ApeKI GCWGC 4 cut(s) 39, 42, 45, 1184
ApoI RAATTY 4 cut(s) 628, 824, 1262, 1572
Asp700I GAANNNNTTC 1 cut(s) 1572
AspLEI GCGC 3 cut(s) 62, 143, 145
AspS9I GGNCC 5 cut(s) 9, 232, 291, 317, 339
AsuC2I CCSGG 8 cut(s) 7, 242, 247, 277, 314, 315, 428, 1509
AsuHPI GGTGA 3 cut(s) 170, 374, 539
AvaI CYCGRG 4 cut(s) 12, 313, 331, 975
AvaII GGWCC 4 cut(s) 9, 232, 291, 317
BaeGI GKGCMC 1 cut(s) 1358
BamHI GGATCC 2 cut(s) 403, 1019
BanI GGYRCC 3 cut(s) 59, 739, 1148
BanII GRGCYC 1 cut(s) 1440
BauI CACGAG 1 cut(s) 942
BbsI GAAGAC 1 cut(s) 27
Bbv12I GWGCWC 2 cut(s) 1358, 1440
BbvI GCAGC 4 cut(s) 51, 54, 57, 1171
BccI CCATC 7 cut(s) 303, 425, 566, 574, 1160, 1231, 1506
BceAI ACGGC 2 cut(s) 47, 355
BciT130I CCWGG 5 cut(s) 753, 866, 924, 1134, 1482
BcnI CCSGG 8 cut(s) 7, 242, 247, 277, 314, 315, 428, 1509
BcoDI GTCTC 3 cut(s) 9, 285, 334
BfaI CTAG 1 cut(s) 707
BfoI RGCGCY 1 cut(s) 63
BglII AGATCT 2 cut(s) 1075, 1120
BisI GCNGC 8 cut(s) 40, 43, 46, 49, 209, 368, 811, 1185
BlsI GCNGC 8 cut(s) 41, 44, 47, 50, 210, 369, 812, 1186
Bme18I GGWCC 4 cut(s) 9, 232, 291, 317
BmeT110I CYCGRG 4 cut(s) 12, 313, 331, 975
BmgBI CACGTC 1 cut(s) 328
BmgT120I GGNCC 5 cut(s) 9, 232, 291, 317, 339
BmrI ACTGGG 1 cut(s) 530
BmsI GCATC 1 cut(s) 502
BmuI ACTGGG 1 cut(s) 530
BpiI GAAGAC 1 cut(s) 27
BplI GAGNNNNNCTC 4 cut(s) 99, 131, 1284, 1316
BpmI CTGGAG 3 cut(s) 143, 518, 804
BpuEI CTTGAG 2 cut(s) 1414, 1454
BpuMI CCSGG 8 cut(s) 7, 242, 247, 277, 314, 315, 428, 1509
BsaAI YACGTR 2 cut(s) 639, 1026
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 285
BsaJI CCNNGG 5 cut(s) 275, 305, 313, 751, 1303
BsaWI WCCGGW 4 cut(s) 217, 265, 319, 1518
BsaXI ACNNNNNCTCC 6 cut(s) 48, 78, 102, 132, 392, 422
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 241, 319, 433, 572, 757
Bse118I RCCGGY 1 cut(s) 24
Bse1I ACTGG 4 cut(s) 501, 525, 597, 787
Bse3DI GCAATG 1 cut(s) 1372
BseAI TCCGGA 1 cut(s) 217
BseBI CCWGG 5 cut(s) 753, 866, 924, 1134, 1482
BseDI CCNNGG 5 cut(s) 275, 305, 313, 751, 1303
BseGI GGATG 5 cut(s) 436, 460, 517, 1223, 1517
BseLI CCNNNNNNNGG 6 cut(s) 89, 241, 319, 433, 572, 757
BseMI GCAATG 1 cut(s) 1372
BseNI ACTGG 4 cut(s) 501, 525, 597, 787
BsePI GCGCGC 1 cut(s) 141
BseRI GAGGAG 3 cut(s) 69, 123, 545
BseSI GKGCMC 1 cut(s) 1358
BseX3I CGGCCG 1 cut(s) 243
BseXI GCAGC 4 cut(s) 51, 54, 57, 1171
BseYI CCCAGC 1 cut(s) 445
BsgI GTGCAG 1 cut(s) 828
Bsh1236I CGCG 1 cut(s) 143
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 245, 341, 620
BshNI GGYRCC 3 cut(s) 59, 739, 1148
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 2 cut(s) 1358, 1440
BsiHKCI CYCGRG 4 cut(s) 12, 313, 331, 975
BsiWI CGTACG 1 cut(s) 408
BslFI GGGAC 2 cut(s) 65, 277
BslI CCNNNNNNNGG 6 cut(s) 89, 241, 319, 433, 572, 757
BsmAI GTCTC 3 cut(s) 9, 285, 334
BsmBI CGTCTC 2 cut(s) 9, 334
BsmFI GGGAC 2 cut(s) 65, 277
BsmI GAATGC 2 cut(s) 508, 857
BsnI GGCC 3 cut(s) 245, 341, 620
Bso31I GGTCTC 1 cut(s) 285
BsoBI CYCGRG 4 cut(s) 12, 313, 331, 975
Bsp1286I GDGCHC 2 cut(s) 1358, 1440
Bsp13I TCCGGA 1 cut(s) 217
Bsp143I GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
BspANI GGCC 3 cut(s) 245, 341, 620
BspEI TCCGGA 1 cut(s) 217
BspFNI CGCG 1 cut(s) 143
BspHI TCATGA 1 cut(s) 66
BspPI GGATC 5 cut(s) 398, 411, 1014, 1027, 1492
BspQI GCTCTTC 1 cut(s) 152
BspT107I GGYRCC 3 cut(s) 59, 739, 1148
BspTNI GGTCTC 1 cut(s) 285
BsrDI GCAATG 1 cut(s) 1372
BsrFI RCCGGY 1 cut(s) 24
BsrI ACTGG 4 cut(s) 501, 525, 597, 787
BssAI RCCGGY 1 cut(s) 24
BssECI CCNNGG 5 cut(s) 275, 305, 313, 751, 1303
BssHII GCGCGC 1 cut(s) 141
BssMI GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
BssNI GRCGYC 1 cut(s) 60
BssSI CACGAG 1 cut(s) 942
Bst2BI CACGAG 1 cut(s) 942
Bst2UI CCWGG 5 cut(s) 753, 866, 924, 1134, 1482
Bst4CI ACNGT 6 cut(s) 182, 716, 1233, 1382, 1418, 1607
Bst6I CTCTTC 3 cut(s) 152, 1032, 1213
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 3 cut(s) 512, 680, 1353
BstBAI YACGTR 2 cut(s) 639, 1026
BstC8I GCNNGC 1 cut(s) 143
BstDEI CTNAG 3 cut(s) 615, 695, 1316
BstDSI CCRYGG 1 cut(s) 1303
BstEII GGTNACC 1 cut(s) 422
BstENI CCTNNNNNAGG 1 cut(s) 87
BstF5I GGATG 5 cut(s) 436, 460, 517, 1223, 1517
BstFNI CGCG 1 cut(s) 143
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 3 cut(s) 62, 143, 145
BstKTI GATC 6 cut(s) 406, 853, 1022, 1078, 1123, 1487
BstMAI GTCTC 3 cut(s) 9, 285, 334
BstMBI GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 8 cut(s) 45, 48, 57, 205, 512, 680, 1353, 1362
BstNI CCWGG 5 cut(s) 753, 866, 924, 1134, 1482
BstNSI RCATGY 1 cut(s) 1596
BstPI GGTNACC 1 cut(s) 422
BstSLI GKGCMC 1 cut(s) 1358
BstSNI TACGTA 1 cut(s) 639
BstUI CGCG 1 cut(s) 143
BstV1I GCAGC 4 cut(s) 51, 54, 57, 1171
BstV2I GAAGAC 1 cut(s) 27
BstX2I RGATCY 5 cut(s) 403, 1019, 1075, 1120, 1484
BstYI RGATCY 5 cut(s) 403, 1019, 1075, 1120, 1484
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 3 cut(s) 245, 341, 620
BtgI CCRYGG 1 cut(s) 1303
BtrI CACGTC 1 cut(s) 328
BtsCI GGATG 5 cut(s) 436, 460, 517, 1223, 1517
BtsI GCAGTG 1 cut(s) 820
BtsIMutI CAGTG 3 cut(s) 721, 820, 1423
Cac8I GCNNGC 1 cut(s) 143
CciI TCATGA 1 cut(s) 66
CfoI GCGC 3 cut(s) 62, 143, 145
Cfr10I RCCGGY 1 cut(s) 24
Cfr13I GGNCC 5 cut(s) 9, 232, 291, 317, 339
Cfr9I CCCGGG 1 cut(s) 313
Csp6I GTAC 3 cut(s) 323, 409, 640
CviAII CATG 6 cut(s) 67, 138, 610, 807, 1051, 1593
CviQI GTAC 3 cut(s) 323, 409, 640
DdeI CTNAG 3 cut(s) 615, 695, 1316
DinI GGCGCC 1 cut(s) 61
DpnI GATC 6 cut(s) 405, 852, 1021, 1077, 1122, 1486
DpnII GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
Eam1104I CTCTTC 3 cut(s) 152, 1032, 1213
EarI CTCTTC 3 cut(s) 152, 1032, 1213
EciI GGCGGA 4 cut(s) 66, 195, 413, 1207
Ecl136II GAGCTC 1 cut(s) 1438
EclXI CGGCCG 1 cut(s) 243
Eco105I TACGTA 1 cut(s) 639
Eco24I GRGCYC 1 cut(s) 1440
Eco31I GGTCTC 1 cut(s) 285
Eco32I GATATC 1 cut(s) 1552
Eco47I GGWCC 4 cut(s) 9, 232, 291, 317
Eco52I CGGCCG 1 cut(s) 243
Eco53kI GAGCTC 1 cut(s) 1438
Eco88I CYCGRG 4 cut(s) 12, 313, 331, 975
Eco91I GGTNACC 1 cut(s) 422
EcoICRI GAGCTC 1 cut(s) 1438
EcoNI CCTNNNNNAGG 1 cut(s) 87
EcoO109I RGGNCCY 1 cut(s) 291
EcoO65I GGTNACC 1 cut(s) 422
EcoRI GAATTC 1 cut(s) 1572
EcoRII CCWGG 5 cut(s) 751, 864, 922, 1132, 1480
EcoRV GATATC 1 cut(s) 1552
EcoT22I ATGCAT 1 cut(s) 1272
EcoT38I GRGCYC 1 cut(s) 1440
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
Esp3I CGTCTC 2 cut(s) 9, 334
FaeI CATG 6 cut(s) 70, 141, 613, 810, 1054, 1596
FalI AAGNNNNNCTT 6 cut(s) 1160, 1192, 1192, 1224, 1416, 1448
FaqI GGGAC 2 cut(s) 65, 277
FatI CATG 6 cut(s) 66, 137, 609, 806, 1050, 1592
FauI CCCGC 1 cut(s) 204
Fnu4HI GCNGC 8 cut(s) 40, 43, 46, 49, 209, 368, 811, 1185
FokI GGATG 5 cut(s) 443, 467, 524, 1210, 1524
FriOI GRGCYC 1 cut(s) 1440
Fsp4HI GCNGC 8 cut(s) 40, 43, 46, 49, 209, 368, 811, 1185
FspBI CTAG 1 cut(s) 707
GlaI GCGC 3 cut(s) 61, 142, 144
GluI GCNGC 8 cut(s) 40, 43, 46, 49, 209, 368, 811, 1185
GsaI CCCAGC 1 cut(s) 449
GsuI CTGGAG 3 cut(s) 143, 518, 804
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 3 cut(s) 245, 341, 620
HhaI GCGC 3 cut(s) 62, 143, 145
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 6 cut(s) 70, 141, 613, 810, 1054, 1596
Hin6I GCGC 3 cut(s) 60, 141, 143
HinP1I GCGC 3 cut(s) 60, 141, 143
HincII GTYRAC 2 cut(s) 1197, 1539
HindII GTYRAC 2 cut(s) 1197, 1539
HindIII AAGCTT 1 cut(s) 697
HinfI GANTC 7 cut(s) 114, 202, 221, 227, 606, 691, 947
HphI GGTGA 3 cut(s) 170, 374, 539
Hpy166II GTNNAC 5 cut(s) 325, 935, 1197, 1356, 1539
Hpy188I TCNGA 6 cut(s) 171, 226, 232, 571, 1255, 1578
Hpy188III TCNNGA 8 cut(s) 67, 218, 485, 944, 965, 1079, 1177, 1282
Hpy8I GTNNAC 5 cut(s) 325, 935, 1197, 1356, 1539
Hpy99I CGWCG 2 cut(s) 416, 1537
HpyAV CCTTC 5 cut(s) 222, 385, 576, 924, 971
HpyCH4III ACNGT 6 cut(s) 182, 716, 1233, 1382, 1418, 1607
HpyCH4IV ACGT 6 cut(s) 175, 327, 638, 877, 1025, 1535
HpyCH4V TGCA 8 cut(s) 506, 515, 674, 683, 845, 1096, 1270, 1356
HpyF10VI GCNNNNNNNGC 8 cut(s) 45, 48, 57, 205, 512, 680, 1353, 1362
HpyF3I CTNAG 3 cut(s) 615, 695, 1316
HpySE526I ACGT 6 cut(s) 175, 327, 638, 877, 1025, 1535
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 6 cut(s) 70, 141, 613, 810, 1054, 1596
HspAI GCGC 3 cut(s) 60, 141, 143
KasI GGCGCC 1 cut(s) 59
Kpn2I TCCGGA 1 cut(s) 217
Kzo9I GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
LguI GCTCTTC 1 cut(s) 152
LmnI GCTCC 5 cut(s) 131, 532, 583, 1305, 1429
Lsp1109I GCAGC 4 cut(s) 51, 54, 57, 1171
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 707
MaeII ACGT 6 cut(s) 175, 327, 638, 877, 1025, 1535
MaeIII GTNAC 6 cut(s) 176, 422, 557, 634, 1324, 1474
MalI GATC 6 cut(s) 405, 852, 1021, 1077, 1122, 1486
MboI GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
MboII GAAGA 7 cut(s) 32, 139, 994, 1049, 1085, 1200, 1479
MfeI CAATTG 2 cut(s) 684, 1340
MflI RGATCY 5 cut(s) 403, 1019, 1075, 1120, 1484
MhlI GDGCHC 2 cut(s) 1358, 1440
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 4 cut(s) 108, 196, 215, 221
MmeI TCCRAC 2 cut(s) 210, 216
Mph1103I ATGCAT 1 cut(s) 1272
MroI TCCGGA 1 cut(s) 217
MroXI GAANNNNTTC 1 cut(s) 1572
MseI TTAA 4 cut(s) 833, 1164, 1287, 1403
MspA1I CMGCKG 3 cut(s) 48, 445, 668
MunI CAATTG 2 cut(s) 684, 1340
Mva1269I GAATGC 2 cut(s) 508, 857
MvaI CCWGG 5 cut(s) 753, 866, 924, 1134, 1482
MvnI CGCG 1 cut(s) 143
MwoI GCNNNNNNNGC 8 cut(s) 45, 48, 57, 205, 512, 680, 1353, 1362
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 8 cut(s) 7, 242, 247, 277, 314, 315, 428, 1509
NdeII GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
NlaIII CATG 6 cut(s) 70, 141, 613, 810, 1054, 1596
NmeAIII GCCGAG 2 cut(s) 102, 330
NmuCI GTSAC 2 cut(s) 176, 1324
NsiI ATGCAT 1 cut(s) 1272
NspI RCATGY 1 cut(s) 1596
PagI TCATGA 1 cut(s) 66
PauI GCGCGC 1 cut(s) 141
PciI ACATGT 1 cut(s) 1592
PciSI GCTCTTC 1 cut(s) 152
PcsI WCGNNNNNNNCGW 1 cut(s) 729
PctI GAATGC 2 cut(s) 508, 857
PdmI GAANNNNTTC 1 cut(s) 1572
PfeI GAWTC 3 cut(s) 606, 691, 947
Pfl23II CGTACG 1 cut(s) 408
PfoI TCCNGGA 1 cut(s) 1480
PkrI GCNGC 8 cut(s) 41, 44, 47, 50, 210, 369, 812, 1186
PleI GAGTC 4 cut(s) 108, 196, 215, 221
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 4 cut(s) 108, 196, 215, 221
Ppu21I YACGTR 2 cut(s) 639, 1026
PpuMI RGGWCCY 1 cut(s) 291
PscI ACATGT 1 cut(s) 1592
PsiI TTATAA 1 cut(s) 902
Psp124BI GAGCTC 1 cut(s) 1440
Psp5II RGGWCCY 1 cut(s) 291
Psp6I CCWGG 5 cut(s) 751, 864, 922, 1132, 1480
PspEI GGTNACC 1 cut(s) 422
PspFI CCCAGC 1 cut(s) 445
PspGI CCWGG 5 cut(s) 751, 864, 922, 1132, 1480
PspLI CGTACG 1 cut(s) 408
PspPI GGNCC 5 cut(s) 9, 232, 291, 317, 339
PspPPI RGGWCCY 1 cut(s) 291
PsuI RGATCY 5 cut(s) 403, 1019, 1075, 1120, 1484
PteI GCGCGC 1 cut(s) 141
PvuII CAGCTG 2 cut(s) 445, 668
RsaI GTAC 3 cut(s) 324, 410, 641
RsaNI GTAC 3 cut(s) 323, 409, 640
SacI GAGCTC 1 cut(s) 1440
SapI GCTCTTC 1 cut(s) 152
SaqAI TTAA 4 cut(s) 833, 1164, 1287, 1403
SatI GCNGC 8 cut(s) 40, 43, 46, 49, 209, 368, 811, 1185
Sau3AI GATC 6 cut(s) 403, 850, 1019, 1075, 1120, 1484
Sau96I GGNCC 5 cut(s) 9, 232, 291, 317, 339
SchI GAGTC 4 cut(s) 108, 196, 215, 221
SduI GDGCHC 2 cut(s) 1358, 1440
SfaNI GCATC 1 cut(s) 502
SfoI GGCGCC 1 cut(s) 61
SinI GGWCC 4 cut(s) 9, 232, 291, 317
SmaI CCCGGG 1 cut(s) 315
SmlI CTYRAG 2 cut(s) 1393, 1433
SmoI CTYRAG 2 cut(s) 1393, 1433
SnaBI TACGTA 1 cut(s) 639
SspDI GGCGCC 1 cut(s) 59
SspI AATATT 1 cut(s) 1582
SspMI CTAG 1 cut(s) 707
SstI GAGCTC 1 cut(s) 1440
TaaI ACNGT 6 cut(s) 182, 716, 1233, 1382, 1418, 1607
TaiI ACGT 6 cut(s) 178, 330, 641, 880, 1028, 1538
TaqI TCGA 6 cut(s) 287, 484, 853, 1080, 1115, 1281
TauI GCSGC 4 cut(s) 51, 211, 370, 813
TfiI GAWTC 3 cut(s) 606, 691, 947
Tru1I TTAA 4 cut(s) 833, 1164, 1287, 1403
Tru9I TTAA 4 cut(s) 833, 1164, 1287, 1403
TscAI CASTG 3 cut(s) 721, 820, 1423
TseFI GTSAC 2 cut(s) 176, 1324
TseI GCWGC 4 cut(s) 39, 42, 45, 1184
Tsp45I GTSAC 2 cut(s) 176, 1324
TspDTI ATGAA 5 cut(s) 471, 598, 980, 1020, 1267
TspGWI ACGGA 3 cut(s) 118, 396, 1292
TspMI CCCGGG 1 cut(s) 313
TspRI CASTG 3 cut(s) 721, 820, 1423
VneI GTGCAC 1 cut(s) 1354
VpaK11BI GGWCC 4 cut(s) 9, 232, 291, 317
XagI CCTNNNNNAGG 1 cut(s) 87
XapI RAATTY 4 cut(s) 628, 824, 1262, 1572
XceI RCATGY 1 cut(s) 1596
XcmI CCANNNNNNNNNTGG 1 cut(s) 569
XmaI CCCGGG 1 cut(s) 313
XmnI GAANNNNTTC 1 cut(s) 1572
XspI CTAG 1 cut(s) 707
Zsp2I ATGCAT 1 cut(s) 1272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.