MD04G1076200.v1.1

aminotransferase ACS10

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0008719
Physical Location & Seq
Forward (+)
10276648 .. 10279425
2778 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1076200.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGACCCGGACCCGGCCCCGGCCCGCCGACCCAAACACCAACCGCTCCAAATCCACTGGCGGAGGCGGCGGAACCACCGTCATGAGAGTTATAGTCCCTCTCCAGGGCGTCGTCCAAGGCAGGGGAGGTCTGGTTTTGGGCTCGCTCATACCGTGCGCCTTGTTCTATTTCCTCCAGCTCTACCTGAAAAGACACCGCTCCAACCCCAACCCGCCTACGCCTCCGCCTTCGCCGGAATCCGAGTCCGAGCAGGCCGGACAGCTGGTGGAACTTCCAGTCCTGCCCAGGTCCCTGTCTCGGACGCTCCTATCCCCGAGGGGCGCGGGTGGACCCGTTTACGTATCGGGTCGGGCCAATTCGGTTCTGAAAGGTGGCGAGTCGCCCTACGACGTTGGGTTGAGGAAGGTGGCGGAGGATCCGTATGACGAGTTGGGTAATCAGAATGGGGTTATTCAATTGGGTTTGGATGAAAACAAGTTATCGTTGGATTTGGTTGGGGATTGGCTTGTGGATAATGCAAAGGATGCGATTCTGGGTGGCGACGAGCTCGGAATTAGTGGTATTGTTTGCTACCAGCCTCTTGATGGATTGATGGAGTTAAAAGTGGCAATGGCAGGATTCATGTCTCAAGTCATGGGGAAGTCAGTTTTCTTCGACCCCTCGCAAATAGTATTGACAGCTGGTGCAACACCTGCAATTGAGATACTAAGTTTCTGCCTAGCAGACAGTGGAAATGCATTTCTTGTTCCGGCGCCATATTACCCTGGTTTGGACAGAGATGTAAAGTGGCGAACTGGAGTAGAGATTATACCTGTTCCATGCCGTAGTGCTGACAAATTCAACTTGAGCATAACTACTCTTGACCGCGCATTCAACCAGGCAAAGAAACGCGGTTTAAAGGTTCGTGGGATTATAATTTCAAATCCTTCAAATCCCGTTGGCAGTTTATATAGTCGTGAATCACTTTACAACCTTCTAGACTTTGCCCGAGAGAAGAACATACATATAATCTCGAATGAAATATTTTCTGGATCCATGCATGGTAGTGAAGAGTTTGTAAGCATGGCAGAAATTGTTGAATTGGAAGATCTGGACCAGAACAGAGTTCATATAGTATATGGTCTATCGAAAGACCTCTCTCTTCCAGGTTTTAGGGCTGGCGCTATCTACTCCTATAACAAGAATGTTCTGGCTGCCGCTAAAAAGTTGACAAGGTTCTCGTCCATTTCCTCCCCATCCCAACGGTTGCTCATCTCTATGCTTTCAGACACCAAATTTATCCGGAAGTTCATTGATACTAGTAGGGAAAGGCTCCGTGCAATGTACCTAAAATTTGTGACAGGTTTGAAGCAATTGGGCATTGAGTGCACAAAGAGCAATGGGGGTTTCAGCTGTTGGGCTGACATGAGCGGGTTAATCCGCTCTTACAGCGAGAAAGGGGAGCTTGAGCTCTGGGATAGGTTGTTGAATGTAGCTAAGCTAAATGTAACTCCCGGTTCATCATGTCATTGTATTGAACCCGGATGGTTTCGGTTTTGTTTTACAACGTTGACTGAAAAAGATATTCCGGTTGTTATGGAACGAATTCGGAAAATTTCCGAAACCTGTAAATCGCACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aminotran_1_2

Protein Analysis

541

Amino Acids

59.16

Weight (kDa)

8.76

Isoelectric Point (pI)

47.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aminotran_1_2 PF00155 152 - 529 5.3e-78 Aminotransferase class I and II
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 914
AarI CACCTGC 1 cut(s) 700
Acc36I ACCTGC 1 cut(s) 700
AccB1I GGYRCC 1 cut(s) 751
AccBSI CCGCTC 4 cut(s) 45, 198, 1410, 1422
AccII CGCG 3 cut(s) 323, 867, 891
AccIII TCCGGA 1 cut(s) 1281
AclI AACGTT 1 cut(s) 1547
AclWI GGATC 4 cut(s) 410, 423, 1026, 1039
AcsI RAATTY 5 cut(s) 836, 1274, 1331, 1584, 1593
AcyI GRCGYC 2 cut(s) 108, 752
AfaI GTAC 1 cut(s) 1325
AfiI CCNNNNNNNGG 8 cut(s) 12, 18, 103, 104, 121, 297, 584, 769
AgsI TTSAA 9 cut(s) 455, 841, 874, 921, 930, 1079, 1348, 1468, 1517
AhlI ACTAGT 1 cut(s) 1298
AjnI CCWGG 5 cut(s) 102, 284, 763, 876, 1144
AloI GAACNNNNNNTCC 2 cut(s) 261, 293
AluBI AGCT 9 cut(s) 178, 262, 547, 680, 1392, 1444, 1450, 1475, 1480
AluI AGCT 9 cut(s) 178, 262, 547, 680, 1392, 1444, 1450, 1475, 1480
Alw21I GWGCWC 3 cut(s) 549, 1370, 1452
Alw26I GTCTC 2 cut(s) 300, 630
Alw44I GTGCAC 1 cut(s) 1366
AlwI GGATC 4 cut(s) 410, 423, 1026, 1039
Ama87I CYCGRG 2 cut(s) 313, 987
Aor13HI TCCGGA 1 cut(s) 1281
AoxI GGCC 4 cut(s) 14, 20, 252, 351
ApaLI GTGCAC 1 cut(s) 1366
ApeKI GCWGC 1 cut(s) 1193
ApoI RAATTY 5 cut(s) 836, 1274, 1331, 1584, 1593
Asp700I GAANNNNTTC 1 cut(s) 1584
AspLEI GCGC 5 cut(s) 158, 323, 754, 869, 1163
AspS9I GGNCC 7 cut(s) 9, 15, 21, 288, 329, 351, 1093
AsuC2I CCSGG 5 cut(s) 7, 13, 19, 1494, 1521
AvaI CYCGRG 2 cut(s) 313, 987
AvaII GGWCC 4 cut(s) 9, 288, 329, 1093
BaeGI GKGCMC 1 cut(s) 1370
BamHI GGATCC 2 cut(s) 415, 1031
BanI GGYRCC 1 cut(s) 751
BanII GRGCYC 3 cut(s) 143, 549, 1452
Bbv12I GWGCWC 3 cut(s) 549, 1370, 1452
BbvI GCAGC 1 cut(s) 1180
BccI CCATC 4 cut(s) 578, 586, 1243, 1518
BceAI ACGGC 1 cut(s) 807
BciT130I CCWGG 5 cut(s) 104, 286, 765, 878, 1146
BcnI CCSGG 5 cut(s) 7, 13, 19, 1494, 1521
BcoDI GTCTC 2 cut(s) 300, 630
BcuI ACTAGT 1 cut(s) 1298
BfaI CTAG 3 cut(s) 719, 977, 1299
BfoI RGCGCY 2 cut(s) 755, 1164
BfuAI ACCTGC 1 cut(s) 700
BglII AGATCT 1 cut(s) 1087
BisI GCNGC 3 cut(s) 67, 1194, 1197
BlpI GCTNAGC 1 cut(s) 1476
BlsI GCNGC 3 cut(s) 68, 1195, 1198
Bme18I GGWCC 4 cut(s) 9, 288, 329, 1093
BmeT110I CYCGRG 2 cut(s) 313, 987
BmgT120I GGNCC 7 cut(s) 9, 15, 21, 288, 329, 351, 1093
BmiI GGNNCC 9 cut(s) 11, 17, 73, 290, 331, 417, 753, 1033, 1313
BmsI GCATC 1 cut(s) 514
BpmI CTGGAG 3 cut(s) 86, 158, 816
Bpu1102I GCTNAGC 1 cut(s) 1476
BpuEI CTTGAG 3 cut(s) 612, 865, 1466
BpuMI CCSGG 5 cut(s) 7, 13, 19, 1494, 1521
BsaAI YACGTR 1 cut(s) 340
BsaHI GRCGYC 2 cut(s) 108, 752
BsaJI CCNNGG 6 cut(s) 17, 103, 115, 284, 314, 763
BsaWI WCCGGW 2 cut(s) 1281, 1567
BsaXI ACNNNNNCTCC 4 cut(s) 117, 147, 404, 434
Bsc4I CCNNNNNNNGG 8 cut(s) 12, 18, 103, 104, 121, 297, 584, 769
Bse1I ACTGG 3 cut(s) 61, 275, 799
Bse3DI GCAATG 3 cut(s) 615, 1326, 1384
BseAI TCCGGA 1 cut(s) 1281
BseBI CCWGG 5 cut(s) 104, 286, 765, 878, 1146
BseDI CCNNGG 6 cut(s) 17, 103, 115, 284, 314, 763
BseGI GGATG 4 cut(s) 472, 529, 1235, 1529
BseLI CCNNNNNNNGG 8 cut(s) 12, 18, 103, 104, 121, 297, 584, 769
BseMI GCAATG 3 cut(s) 615, 1326, 1384
BseNI ACTGG 3 cut(s) 61, 275, 799
BseSI GKGCMC 1 cut(s) 1370
BseXI GCAGC 1 cut(s) 1180
Bsh1236I CGCG 3 cut(s) 323, 867, 891
BshFI GGCC 4 cut(s) 16, 22, 254, 353
BshNI GGYRCC 1 cut(s) 751
BsiHKAI GWGCWC 3 cut(s) 549, 1370, 1452
BsiHKCI CYCGRG 2 cut(s) 313, 987
BslFI GGGAC 2 cut(s) 80, 274
BslI CCNNNNNNNGG 8 cut(s) 12, 18, 103, 104, 121, 297, 584, 769
BsmAI GTCTC 2 cut(s) 300, 630
BsmFI GGGAC 2 cut(s) 80, 274
BsmI GAATGC 1 cut(s) 869
BsnI GGCC 4 cut(s) 16, 22, 254, 353
BsoBI CYCGRG 2 cut(s) 313, 987
Bsp1286I GDGCHC 4 cut(s) 143, 549, 1370, 1452
Bsp13I TCCGGA 1 cut(s) 1281
Bsp143I GATC 3 cut(s) 415, 1031, 1087
Bsp1720I GCTNAGC 1 cut(s) 1476
BspANI GGCC 4 cut(s) 16, 22, 254, 353
BspEI TCCGGA 1 cut(s) 1281
BspFNI CGCG 3 cut(s) 323, 867, 891
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 9 cut(s) 11, 17, 73, 290, 331, 417, 753, 1033, 1313
BspMI ACCTGC 1 cut(s) 700
BspPI GGATC 4 cut(s) 410, 423, 1026, 1039
BspT107I GGYRCC 1 cut(s) 751
BsrBI CCGCTC 4 cut(s) 45, 198, 1410, 1422
BsrDI GCAATG 3 cut(s) 615, 1326, 1384
BsrI ACTGG 3 cut(s) 61, 275, 799
BssECI CCNNGG 6 cut(s) 17, 103, 115, 284, 314, 763
BssMI GATC 3 cut(s) 415, 1031, 1087
BssNI GRCGYC 2 cut(s) 108, 752
BssT1I CCWWGG 1 cut(s) 115
Bst2UI CCWGG 5 cut(s) 104, 286, 765, 878, 1146
Bst4CI ACNGT 5 cut(s) 79, 153, 728, 1245, 1619
Bst6I CTCTTC 2 cut(s) 1044, 1146
BstACI GRCGYC 2 cut(s) 108, 752
BstAPI GCANNNNNTGC 3 cut(s) 524, 692, 1365
BstBAI YACGTR 1 cut(s) 340
BstC8I GCNNGC 4 cut(s) 24, 143, 252, 1159
BstDEI CTNAG 2 cut(s) 707, 1476
BstF5I GGATG 4 cut(s) 472, 529, 1235, 1529
BstFNI CGCG 3 cut(s) 323, 867, 891
BstH2I RGCGCY 2 cut(s) 755, 1164
BstHHI GCGC 5 cut(s) 158, 323, 754, 869, 1163
BstKTI GATC 3 cut(s) 418, 1034, 1090
BstMAI GTCTC 2 cut(s) 300, 630
BstMBI GATC 3 cut(s) 415, 1031, 1087
BstMWI GCNNNNNNNGC 6 cut(s) 66, 524, 692, 1365, 1374, 1428
BstNI CCWGG 5 cut(s) 104, 286, 765, 878, 1146
BstSLI GKGCMC 1 cut(s) 1370
BstSNI TACGTA 1 cut(s) 340
BstUI CGCG 3 cut(s) 323, 867, 891
BstV1I GCAGC 1 cut(s) 1180
BstX2I RGATCY 3 cut(s) 415, 1031, 1087
BstYI RGATCY 3 cut(s) 415, 1031, 1087
BsuRI GGCC 4 cut(s) 16, 22, 254, 353
BtsCI GGATG 4 cut(s) 472, 529, 1235, 1529
BtsIMutI CAGTG 2 cut(s) 54, 733
BveI ACCTGC 1 cut(s) 700
Cac8I GCNNGC 4 cut(s) 24, 143, 252, 1159
CciI TCATGA 1 cut(s) 81
CfoI GCGC 5 cut(s) 158, 323, 754, 869, 1163
Cfr13I GGNCC 7 cut(s) 9, 15, 21, 288, 329, 351, 1093
CseI GACGC 2 cut(s) 97, 310
Csp6I GTAC 1 cut(s) 1324
CviAII CATG 9 cut(s) 82, 622, 634, 819, 1036, 1040, 1063, 1405, 1503
CviQI GTAC 1 cut(s) 1324
DdeI CTNAG 2 cut(s) 707, 1476
DinI GGCGCC 1 cut(s) 753
DpnI GATC 3 cut(s) 417, 1033, 1089
DpnII GATC 3 cut(s) 415, 1031, 1087
DraI TTTAAA 1 cut(s) 897
Eam1104I CTCTTC 2 cut(s) 1044, 1146
EarI CTCTTC 2 cut(s) 1044, 1146
EciI GGCGGA 4 cut(s) 75, 84, 213, 425
Ecl136II GAGCTC 2 cut(s) 547, 1450
Eco105I TACGTA 1 cut(s) 340
Eco130I CCWWGG 1 cut(s) 115
Eco24I GRGCYC 3 cut(s) 143, 549, 1452
Eco47I GGWCC 4 cut(s) 9, 288, 329, 1093
Eco53kI GAGCTC 2 cut(s) 547, 1450
Eco88I CYCGRG 2 cut(s) 313, 987
EcoICRI GAGCTC 2 cut(s) 547, 1450
EcoO109I RGGNCCY 1 cut(s) 288
EcoRI GAATTC 1 cut(s) 1584
EcoRII CCWGG 5 cut(s) 102, 284, 763, 876, 1144
EcoT14I CCWWGG 1 cut(s) 115
EcoT22I ATGCAT 2 cut(s) 739, 1041
EcoT38I GRGCYC 3 cut(s) 143, 549, 1452
EgeI GGCGCC 1 cut(s) 753
EheI GGCGCC 1 cut(s) 753
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 9 cut(s) 85, 625, 637, 822, 1039, 1043, 1066, 1408, 1506
FalI AAGNNNNNCTT 2 cut(s) 1428, 1460
FaqI GGGAC 2 cut(s) 80, 274
FatI CATG 9 cut(s) 81, 621, 633, 818, 1035, 1039, 1062, 1404, 1502
FauI CCCGC 4 cut(s) 31, 219, 316, 1403
Fnu4HI GCNGC 3 cut(s) 67, 1194, 1197
FokI GGATG 4 cut(s) 479, 536, 1222, 1536
FriOI GRGCYC 3 cut(s) 143, 549, 1452
Fsp4HI GCNGC 3 cut(s) 67, 1194, 1197
FspBI CTAG 3 cut(s) 719, 977, 1299
GlaI GCGC 5 cut(s) 157, 322, 753, 868, 1162
GluI GCNGC 3 cut(s) 67, 1194, 1197
GsuI CTGGAG 3 cut(s) 86, 158, 816
HaeII RGCGCY 2 cut(s) 755, 1164
HaeIII GGCC 4 cut(s) 16, 22, 254, 353
HgaI GACGC 2 cut(s) 97, 310
HhaI GCGC 5 cut(s) 158, 323, 754, 869, 1163
Hin1I GRCGYC 2 cut(s) 108, 752
Hin1II CATG 9 cut(s) 85, 625, 637, 822, 1039, 1043, 1066, 1408, 1506
Hin6I GCGC 5 cut(s) 156, 321, 752, 867, 1161
HinP1I GCGC 5 cut(s) 156, 321, 752, 867, 1161
HincII GTYRAC 2 cut(s) 1209, 1551
HindII GTYRAC 2 cut(s) 1209, 1551
HinfI GANTC 6 cut(s) 236, 242, 377, 529, 618, 959
Hpy166II GTNNAC 5 cut(s) 329, 337, 1209, 1368, 1551
Hpy188I TCNGA 9 cut(s) 241, 247, 300, 366, 441, 551, 1267, 1590, 1600
Hpy188III TCNNGA 9 cut(s) 82, 581, 860, 956, 977, 1012, 1029, 1091, 1282
Hpy8I GTNNAC 5 cut(s) 329, 337, 1209, 1368, 1551
Hpy99I CGWCG 3 cut(s) 113, 392, 545
HpyAV CCTTC 4 cut(s) 237, 397, 936, 983
HpyCH4III ACNGT 5 cut(s) 79, 153, 728, 1245, 1619
HpyCH4IV ACGT 3 cut(s) 339, 390, 1547
HpyCH4V TGCA 7 cut(s) 518, 686, 695, 737, 1039, 1319, 1368
HpyF10VI GCNNNNNNNGC 6 cut(s) 66, 524, 692, 1365, 1374, 1428
HpyF3I CTNAG 2 cut(s) 707, 1476
HpySE526I ACGT 3 cut(s) 339, 390, 1547
Hsp92I GRCGYC 2 cut(s) 108, 752
Hsp92II CATG 9 cut(s) 85, 625, 637, 822, 1039, 1043, 1066, 1408, 1506
HspAI GCGC 5 cut(s) 156, 321, 752, 867, 1161
KasI GGCGCC 1 cut(s) 751
Kpn2I TCCGGA 1 cut(s) 1281
Kzo9I GATC 3 cut(s) 415, 1031, 1087
LmnI GCTCC 5 cut(s) 50, 203, 309, 1317, 1441
Lsp1109I GCAGC 1 cut(s) 1180
LweI GCATC 1 cut(s) 514
MaeI CTAG 3 cut(s) 719, 977, 1299
MaeII ACGT 3 cut(s) 339, 390, 1547
MaeIII GTNAC 2 cut(s) 1336, 1486
MalI GATC 3 cut(s) 417, 1033, 1089
MbiI CCGCTC 4 cut(s) 45, 198, 1410, 1422
MboI GATC 3 cut(s) 415, 1031, 1087
MboII GAAGA 5 cut(s) 643, 1006, 1061, 1097, 1133
MfeI CAATTG 3 cut(s) 455, 696, 1352
MflI RGATCY 3 cut(s) 415, 1031, 1087
MhlI GDGCHC 4 cut(s) 143, 549, 1370, 1452
Mly113I GGCGCC 1 cut(s) 752
MlyI GAGTC 2 cut(s) 251, 386
MmeI TCCRAC 2 cut(s) 225, 465
Mph1103I ATGCAT 2 cut(s) 739, 1041
MroI TCCGGA 1 cut(s) 1281
MroXI GAANNNNTTC 1 cut(s) 1584
MseI TTAA 3 cut(s) 599, 896, 1415
MslI CAYNNNNRTG 3 cut(s) 80, 1044, 1256
MspA1I CMGCKG 3 cut(s) 262, 680, 1392
MunI CAATTG 3 cut(s) 455, 696, 1352
Mva1269I GAATGC 1 cut(s) 869
MvaI CCWGG 5 cut(s) 104, 286, 765, 878, 1146
MvnI CGCG 3 cut(s) 323, 867, 891
MwoI GCNNNNNNNGC 6 cut(s) 66, 524, 692, 1365, 1374, 1428
NarI GGCGCC 1 cut(s) 752
NciI CCSGG 5 cut(s) 7, 13, 19, 1494, 1521
NdeII GATC 3 cut(s) 415, 1031, 1087
NlaIII CATG 9 cut(s) 85, 625, 637, 822, 1039, 1043, 1066, 1408, 1506
NlaIV GGNNCC 9 cut(s) 11, 17, 73, 290, 331, 417, 753, 1033, 1313
NmuCI GTSAC 1 cut(s) 1336
NsiI ATGCAT 2 cut(s) 739, 1041
PagI TCATGA 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 700
PcsI WCGNNNNNNNCGW 1 cut(s) 149
PctI GAATGC 1 cut(s) 869
PdmI GAANNNNTTC 1 cut(s) 1584
PfeI GAWTC 4 cut(s) 236, 529, 618, 959
PkrI GCNGC 3 cut(s) 68, 1195, 1198
PleI GAGTC 2 cut(s) 250, 385
PluTI GGCGCC 1 cut(s) 755
PpsI GAGTC 2 cut(s) 250, 385
Ppu21I YACGTR 1 cut(s) 340
PpuMI RGGWCCY 1 cut(s) 288
PsiI TTATAA 1 cut(s) 914
Psp124BI GAGCTC 2 cut(s) 549, 1452
Psp1406I AACGTT 1 cut(s) 1547
Psp5II RGGWCCY 1 cut(s) 288
Psp6I CCWGG 5 cut(s) 102, 284, 763, 876, 1144
PspGI CCWGG 5 cut(s) 102, 284, 763, 876, 1144
PspN4I GGNNCC 9 cut(s) 11, 17, 73, 290, 331, 417, 753, 1033, 1313
PspPI GGNCC 7 cut(s) 9, 15, 21, 288, 329, 351, 1093
PspPPI RGGWCCY 1 cut(s) 288
PsuI RGATCY 3 cut(s) 415, 1031, 1087
PvuII CAGCTG 3 cut(s) 262, 680, 1392
RsaI GTAC 1 cut(s) 1325
RsaNI GTAC 1 cut(s) 1324
RseI CAYNNNNRTG 3 cut(s) 80, 1044, 1256
SacI GAGCTC 2 cut(s) 549, 1452
SaqAI TTAA 3 cut(s) 599, 896, 1415
SatI GCNGC 3 cut(s) 67, 1194, 1197
Sau3AI GATC 3 cut(s) 415, 1031, 1087
Sau96I GGNCC 7 cut(s) 9, 15, 21, 288, 329, 351, 1093
SchI GAGTC 2 cut(s) 251, 386
SduI GDGCHC 4 cut(s) 143, 549, 1370, 1452
SfaNI GCATC 1 cut(s) 514
SfoI GGCGCC 1 cut(s) 753
SinI GGWCC 4 cut(s) 9, 288, 329, 1093
SmiMI CAYNNNNRTG 3 cut(s) 80, 1044, 1256
SmlI CTYRAG 3 cut(s) 627, 844, 1445
SmoI CTYRAG 3 cut(s) 627, 844, 1445
SnaBI TACGTA 1 cut(s) 340
SpeI ACTAGT 1 cut(s) 1298
SspDI GGCGCC 1 cut(s) 751
SspI AATATT 1 cut(s) 1023
SspMI CTAG 3 cut(s) 719, 977, 1299
SstI GAGCTC 2 cut(s) 549, 1452
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 5 cut(s) 79, 153, 728, 1245, 1619
TaiI ACGT 3 cut(s) 342, 393, 1550
TaqI TCGA 3 cut(s) 654, 1013, 1127
TauI GCSGC 2 cut(s) 69, 1199
TfiI GAWTC 4 cut(s) 236, 529, 618, 959
Tru1I TTAA 3 cut(s) 599, 896, 1415
Tru9I TTAA 3 cut(s) 599, 896, 1415
TscAI CASTG 2 cut(s) 61, 733
TseFI GTSAC 1 cut(s) 1336
TseI GCWGC 1 cut(s) 1193
Tsp45I GTSAC 1 cut(s) 1336
TspDTI ATGAA 6 cut(s) 483, 610, 1032, 1097, 1279, 1488
TspGWI ACGGA 2 cut(s) 408, 1304
TspRI CASTG 2 cut(s) 61, 733
VneI GTGCAC 1 cut(s) 1366
VpaK11BI GGWCC 4 cut(s) 9, 288, 329, 1093
XapI RAATTY 5 cut(s) 836, 1274, 1331, 1584, 1593
XbaI TCTAGA 1 cut(s) 976
XcmI CCANNNNNNNNNTGG 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 1584
XspI CTAG 3 cut(s) 719, 977, 1299
Zsp2I ATGCAT 2 cut(s) 739, 1041
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.