Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008447

Physical Location & Seq

Genomic Coordinates Reverse (-)

23742842 .. 23745385

Gene Structure 2544 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G63990.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1152 bp

ATGGAGGAAAGTTCAGGACTATCATCGATGAAGTTCTTCTCCGATCAACACCTCTCTTACGCTGACATTCTTCTTCCTCACGAAGCTCGTGCTAGAATCGAAGTTTCTGTTCTCAATCTCCTCCGAATTTTGAACTCCCCAGATCCAGCTATCTCCGATCTCTCTCTGATCAATAGGAAGAGAAGCAATAGTTGTATAAACAAAGGGATACTCACAGATGTTTCATATATATTCCTCTCTACTTCGTTTACTAAGAGTTCATTGACGAATGCTAAAACAGCTAAAGCTTTTGTTCGTGTGTGGAAAGTTATGGAAATATGCTTTCAGATTCTGCTTCAAGAGAAACGAGTCACACAAAGAGAGCTCTTCTATAAGTTGCTTTGTGATTCACCTGATTACTTTTCATCTCAGATTGAAGTTAACAGAAGTGTCCAAGATGTGGTAGCACTTCTACGTTGTAGTAGATACAGTCTTGGTATTATGGCTTCAAGCAGAGGCCTTGTTGCTGGAAGGTTATTTCTACAAGAACCAGGTAAGGAAGCTGTAGATTGTTCGGCCTGTGGTTCTTCAGGTTTTGCTATAACTGGAGACTTGAATTTGCTAGACAATACCATCATGAGAACTGATGCTCGTTATATCATTATTGTGGAAAAGCATGCGATCTTTCATCGGCTCGTGGAAGATCGTGTGTTCAATCACATTCCTTGCGTGTTCATCACAGCGAAAGGGTATCCGGATATTGCCACAAGGTTTTTCCTCCACCGGATGAGCACAACTTTTCCTGATCTGCCAATTCTTGTTCTAGTTGATTGGAATCCAGCTGGGTTAGCTATACTATGCACCTTCAAGTTTGGAAGCATAGGGATGGGACTTGAAGCATACAGATACGCTTGCAATGTGAAGTGGATTGGTCTCCGAGGAGATGATCTGAATCTGATACCAGAAGAGTCTTTGGTTCCCTTAAAGCCAAAAGATTCACAGATTGCTAAGAGCTTATTGTCCTCCAAAATATTGCAGGAAAACTACATAGAGGAGTTGTCACTGATGGTTCAAACTGGTAAAAGAGCGGAAATTGAAGCTCTCTATTGTCATGGTTATAATTATCTCGGTAAATATATAGCTACCAAGATCGTGCAAGGCAAATACATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0009553 GO:0009555 GO:0009791 GO:0009987 GO:0010154 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061458 GO:0061505 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

383

Amino Acids

43.13

Weight (kDa)

8.57

Isoelectric Point (pI)

48.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TP6A_N	PF04406	96 - 160	4.5e-16	Type IIB DNA topoisomerase
TOP6A-Spo11_Toprim	PF21180	212 - 270	2.4e-17	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63990 AT1G63990
fragaria_vesca	FvH4_5g03200 FvH4_5g03200 FvH4_5g03200 FvH4_5g03200 FvH4_5g03200
malus_domestica	MD14G1112000.v1.1
prunus_persica	Prupe.5G106600_v2.0.a1
pyrus_communis	pycom14g10670
rosa_chinensis	RchiOBHm_Chr7g0196581
rosa_multiflora	Rmu_co8280957.1_g000001 Rmu_sc0006080.1_g000002 Rmu_sc0040598.1_g000001 Rmu_sc0040599.1_g000001
rosa_roxburghii	Rroxscaffold_3G00259190
rosa_rugosa	Rorug07G0034800 Rorug07G0034900
rosa_samantha	Rh7AG158900 Rh7BG160900 Rh7CG167300 Rh7DG160100
rosa_wichuraiana	Rw7G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1098
AccB7I	CCANNNNNTGG	1 cut(s)	439
AccBSI	CCGCTC	1 cut(s)	1067
AccIII	TCCGGA	1 cut(s)	733
AciI	CCGC	1 cut(s)	1067
AclWI	GGATC	1 cut(s)	137
AcsI	RAATTY	2 cut(s)	126, 595
AcuI	CTGAAG	1 cut(s)	552
AfiI	CCNNNNNNNGG	1 cut(s)	439
AjnI	CCWGG	1 cut(s)	529
Alw21I	GWGCWC	2 cut(s)	366, 773
Alw26I	GTCTC	2 cut(s)	582, 917
AlwI	GGATC	1 cut(s)	137
AlwNI	CAGNNNCTG	1 cut(s)	331
Aor13HI	TCCGGA	1 cut(s)	733
AoxI	GGCC	2 cut(s)	496, 555
ApoI	RAATTY	2 cut(s)	126, 595
Asp700I	GAANNNNTTC	1 cut(s)	35
AsuHPI	GGTGA	1 cut(s)	381
BanII	GRGCYC	1 cut(s)	366
BarI	GAAGNNNNNNTAC	2 cut(s)	469, 501
BauI	CACGAG	2 cut(s)	87, 674
Bbv12I	GWGCWC	2 cut(s)	366, 773
BccI	CCATC	3 cut(s)	620, 859, 1039
BcgI	CGANNNNNNTGC	2 cut(s)	71, 105
BciT130I	CCWGG	1 cut(s)	531
BciVI	GTATCC	2 cut(s)	201, 741
BclI	TGATCA	1 cut(s)	168
BcoDI	GTCTC	2 cut(s)	582, 917
BfaI	CTAG	3 cut(s)	93, 602, 803
BfmI	CTRYAG	1 cut(s)	543
BfuI	GTATCC	2 cut(s)	201, 741
Bme1390I	CCNGG	1 cut(s)	531
BmiI	GGNNCC	1 cut(s)	957
BmrFI	CCNGG	1 cut(s)	531
BmsI	GCATC	1 cut(s)	616
BpmI	CTGGAG	1 cut(s)	606
Bsa29I	ATCGAT	1 cut(s)	26
BsaBI	GATNNNNATC	2 cut(s)	813, 930
BsaI	GGTCTC	1 cut(s)	917
BsaJI	CCNNGG	1 cut(s)	916
BsaWI	WCCGGW	2 cut(s)	733, 762
Bsc4I	CCNNNNNNNGG	1 cut(s)	439
Bse1I	ACTGG	2 cut(s)	589, 1060
Bse3DI	GCAATG	1 cut(s)	901
Bse8I	GATNNNNATC	2 cut(s)	813, 930
BseAI	TCCGGA	1 cut(s)	733
BseBI	CCWGG	1 cut(s)	531
BseCI	ATCGAT	1 cut(s)	26
BseDI	CCNNGG	1 cut(s)	916
BseGI	GGATG	2 cut(s)	771, 870
BseJI	GATNNNNATC	2 cut(s)	813, 930
BseLI	CCNNNNNNNGG	1 cut(s)	439
BseMI	GCAATG	1 cut(s)	901
BseMII	CTCAG	1 cut(s)	422
BseNI	ACTGG	2 cut(s)	589, 1060
BseRI	GAGGAG	3 cut(s)	110, 933, 1046
BseYI	CCCAGC	1 cut(s)	821
BshFI	GGCC	2 cut(s)	498, 557
BshVI	ATCGAT	1 cut(s)	26
BsiHKAI	GWGCWC	2 cut(s)	366, 773
BsiSI	CCGG	2 cut(s)	734, 763
BslFI	GGGAC	1 cut(s)	882
BslI	CCNNNNNNNGG	1 cut(s)	439
BsmAI	GTCTC	2 cut(s)	582, 917
BsmFI	GGGAC	1 cut(s)	882
BsmI	GAATGC	1 cut(s)	274
BsnI	GGCC	2 cut(s)	498, 557
Bso31I	GGTCTC	1 cut(s)	917
Bsp1286I	GDGCHC	2 cut(s)	366, 773
Bsp13I	TCCGGA	1 cut(s)	733
Bsp143I	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
BspACI	CCGC	1 cut(s)	1067
BspANI	GGCC	2 cut(s)	498, 557
BspCNI	CTCAG	1 cut(s)	421
BspDI	ATCGAT	1 cut(s)	26
BspEI	TCCGGA	1 cut(s)	733
BspHI	TCATGA	1 cut(s)	615
BspLI	GGNNCC	1 cut(s)	957
BspPI	GGATC	1 cut(s)	137
BspQI	GCTCTTC	1 cut(s)	371
BspTNI	GGTCTC	1 cut(s)	917
BsrBI	CCGCTC	1 cut(s)	1067
BsrDI	GCAATG	1 cut(s)	901
BsrI	ACTGG	2 cut(s)	589, 1060
BssECI	CCNNGG	1 cut(s)	916
BssMI	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
BssSI	CACGAG	2 cut(s)	87, 674
Bst2BI	CACGAG	2 cut(s)	87, 674
Bst2UI	CCWGG	1 cut(s)	531
Bst4CI	ACNGT	1 cut(s)	470
Bst6I	CTCTTC	3 cut(s)	173, 371, 939
BstC8I	GCNNGC	2 cut(s)	657, 892
BstDEI	CTNAG	3 cut(s)	252, 408, 987
BstF5I	GGATG	2 cut(s)	771, 870
BstKTI	GATC	9 cut(s)	46, 145, 160, 171, 663, 685, 787, 928, 1131
BstMAI	GTCTC	2 cut(s)	582, 917
BstMBI	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
BstMWI	GCNNNNNNNGC	2 cut(s)	278, 827
BstNI	CCWGG	1 cut(s)	531
BstNSI	RCATGY	1 cut(s)	659
BstSCI	CCNGG	1 cut(s)	529
BstSFI	CTRYAG	1 cut(s)	543
BstX2I	RGATCY	1 cut(s)	142
BstYI	RGATCY	1 cut(s)	142
Bsu15I	ATCGAT	1 cut(s)	26
BsuI	GTATCC	2 cut(s)	201, 741
BsuRI	GGCC	2 cut(s)	498, 557
BsuTUI	ATCGAT	1 cut(s)	26
BtsCI	GGATG	2 cut(s)	771, 870
BtsIMutI	CAGTG	1 cut(s)	1040
Cac8I	GCNNGC	2 cut(s)	657, 892
CaiI	CAGNNNCTG	1 cut(s)	331
CciI	TCATGA	1 cut(s)	615
ClaI	ATCGAT	1 cut(s)	26
CsiI	ACCWGGT	1 cut(s)	529
CviAII	CATG	3 cut(s)	616, 656, 1091
DdeI	CTNAG	3 cut(s)	252, 408, 987
DpnI	GATC	9 cut(s)	45, 144, 159, 170, 662, 684, 786, 927, 1130
DpnII	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
Eam1104I	CTCTTC	3 cut(s)	173, 371, 939
EarI	CTCTTC	3 cut(s)	173, 371, 939
Ecl136II	GAGCTC	1 cut(s)	364
Eco147I	AGGCCT	1 cut(s)	498
Eco24I	GRGCYC	1 cut(s)	366
Eco31I	GGTCTC	1 cut(s)	917
Eco53kI	GAGCTC	1 cut(s)	364
Eco57I	CTGAAG	1 cut(s)	552
EcoICRI	GAGCTC	1 cut(s)	364
EcoRII	CCWGG	1 cut(s)	529
EcoT38I	GRGCYC	1 cut(s)	366
FaeI	CATG	3 cut(s)	619, 659, 1094
FaqI	GGGAC	1 cut(s)	882
FatI	CATG	3 cut(s)	615, 655, 1090
FbaI	TGATCA	1 cut(s)	168
FokI	GGATG	2 cut(s)	778, 877
FriOI	GRGCYC	1 cut(s)	366
FspBI	CTAG	3 cut(s)	93, 602, 803
GsaI	CCCAGC	1 cut(s)	825
GsuI	CTGGAG	1 cut(s)	606
HaeIII	GGCC	2 cut(s)	498, 557
HapII	CCGG	2 cut(s)	734, 763
Hin1II	CATG	3 cut(s)	619, 659, 1094
HincII	GTYRAC	1 cut(s)	421
HindII	GTYRAC	1 cut(s)	421
HindIII	AAGCTT	1 cut(s)	285
HinfI	GANTC	8 cut(s)	96, 328, 348, 386, 814, 931, 947, 974
HpaI	GTTAAC	1 cut(s)	421
HpaII	CCGG	2 cut(s)	734, 763
HphI	GGTGA	1 cut(s)	381
Hpy166II	GTNNAC	2 cut(s)	249, 421
Hpy188I	TCNGA	9 cut(s)	43, 125, 157, 168, 327, 411, 917, 930, 936
Hpy188III	TCNNGA	6 cut(s)	15, 80, 338, 616, 734, 782
Hpy8I	GTNNAC	2 cut(s)	249, 421
HpyAV	CCTTC	2 cut(s)	504, 853
HpyCH4III	ACNGT	1 cut(s)	470
HpyCH4IV	ACGT	1 cut(s)	454
HpyCH4V	TGCA	4 cut(s)	840, 894, 1015, 1135
HpyF10VI	GCNNNNNNNGC	2 cut(s)	278, 827
HpyF3I	CTNAG	3 cut(s)	252, 408, 987
HpySE526I	ACGT	1 cut(s)	454
Hsp92II	CATG	3 cut(s)	619, 659, 1094
Kpn2I	TCCGGA	1 cut(s)	733
Ksp22I	TGATCA	1 cut(s)	168
KspAI	GTTAAC	1 cut(s)	421
Kzo9I	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
LguI	GCTCTTC	1 cut(s)	371
LweI	GCATC	1 cut(s)	616
MabI	ACCWGGT	1 cut(s)	529
MaeI	CTAG	3 cut(s)	93, 602, 803
MaeII	ACGT	1 cut(s)	454
MaeIII	GTNAC	2 cut(s)	349, 1038
MalI	GATC	9 cut(s)	45, 144, 159, 170, 662, 684, 786, 927, 1130
MbiI	CCGCTC	1 cut(s)	1067
MboI	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
MboII	GAAGA	8 cut(s)	28, 62, 65, 190, 358, 558, 692, 956
MflI	RGATCY	1 cut(s)	142
MhlI	GDGCHC	2 cut(s)	366, 773
MluCI	AATT	5 cut(s)	126, 595, 792, 1071, 1099
MlyI	GAGTC	2 cut(s)	357, 956
MnlI	CCTC	9 cut(s)	62, 87, 131, 245, 488, 767, 911, 1012, 1024
MroI	TCCGGA	1 cut(s)	733
MroXI	GAANNNNTTC	1 cut(s)	35
MseI	TTAA	2 cut(s)	420, 962
MslI	CAYNNNNRTG	2 cut(s)	644, 863
MspA1I	CMGCKG	1 cut(s)	821
MspI	CCGG	2 cut(s)	734, 763
MspR9I	CCNGG	1 cut(s)	531
Mva1269I	GAATGC	1 cut(s)	274
MvaI	CCWGG	1 cut(s)	531
MwoI	GCNNNNNNNGC	2 cut(s)	278, 827
NdeII	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
NlaIII	CATG	3 cut(s)	619, 659, 1094
NlaIV	GGNNCC	1 cut(s)	957
NmuCI	GTSAC	2 cut(s)	349, 1038
NspI	RCATGY	1 cut(s)	659
PaeI	GCATGC	1 cut(s)	659
PagI	TCATGA	1 cut(s)	615
PceI	AGGCCT	1 cut(s)	498
PciSI	GCTCTTC	1 cut(s)	371
PctI	GAATGC	1 cut(s)	274
PdmI	GAANNNNTTC	1 cut(s)	35
PfeI	GAWTC	6 cut(s)	96, 328, 386, 814, 931, 974
PflMI	CCANNNNNTGG	1 cut(s)	439
PleI	GAGTC	2 cut(s)	356, 955
PpsI	GAGTC	2 cut(s)	356, 955
PsiI	TTATAA	1 cut(s)	1098
Psp124BI	GAGCTC	1 cut(s)	366
Psp6I	CCWGG	1 cut(s)	529
PspFI	CCCAGC	1 cut(s)	821
PspGI	CCWGG	1 cut(s)	529
PspN4I	GGNNCC	1 cut(s)	957
PstNI	CAGNNNCTG	1 cut(s)	331
PsuI	RGATCY	1 cut(s)	142
PvuII	CAGCTG	1 cut(s)	821
RseI	CAYNNNNRTG	2 cut(s)	644, 863
SacI	GAGCTC	1 cut(s)	366
SapI	GCTCTTC	1 cut(s)	371
SaqAI	TTAA	2 cut(s)	420, 962
Sau3AI	GATC	9 cut(s)	43, 142, 157, 168, 660, 682, 784, 925, 1128
SchI	GAGTC	2 cut(s)	357, 956
ScrFI	CCNGG	1 cut(s)	531
SduI	GDGCHC	2 cut(s)	366, 773
SexAI	ACCWGGT	1 cut(s)	529
SfaNI	GCATC	1 cut(s)	616
SfcI	CTRYAG	1 cut(s)	543
SmiMI	CAYNNNNRTG	2 cut(s)	644, 863
SphI	GCATGC	1 cut(s)	659
Sse9I	AATT	5 cut(s)	126, 595, 792, 1071, 1099
SseBI	AGGCCT	1 cut(s)	498
SsiI	CCGC	1 cut(s)	1067
SspI	AATATT	1 cut(s)	1011
SspMI	CTAG	3 cut(s)	93, 602, 803
SstI	GAGCTC	1 cut(s)	366
StuI	AGGCCT	1 cut(s)	498
StyD4I	CCNGG	1 cut(s)	529
TaaI	ACNGT	1 cut(s)	470
TaiI	ACGT	1 cut(s)	457
TaqI	TCGA	2 cut(s)	26, 99
TasI	AATT	5 cut(s)	126, 595, 792, 1071, 1099
TfiI	GAWTC	6 cut(s)	96, 328, 386, 814, 931, 974
Tru1I	TTAA	2 cut(s)	420, 962
Tru9I	TTAA	2 cut(s)	420, 962
TscAI	CASTG	1 cut(s)	1047
TseFI	GTSAC	2 cut(s)	349, 1038
Tsp45I	GTSAC	2 cut(s)	349, 1038
TspDTI	ATGAA	6 cut(s)	44, 213, 249, 393, 656, 703
TspRI	CASTG	1 cut(s)	1047
Van91I	CCANNNNNTGG	1 cut(s)	439
XapI	RAATTY	2 cut(s)	126, 595
XceI	RCATGY	1 cut(s)	659
XmnI	GAANNNNTTC	1 cut(s)	35
XspI	CTAG	3 cut(s)	93, 602, 803

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.