Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0008447

Physical Location & Seq

Genomic Coordinates Reverse (-)

17988235 .. 17990461

Gene Structure 2227 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1112000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGGAAGACGACTGCAAATCCTCCTTGAATTTCTTCTCCGATCAACAGCTCTGCTACGCCGACATCCTCGCTCCGCCGGAGGCCATTGCCCGAATCCAAGTCGCCGTCCTCAACTTCCTCCGAATCCTGACCTCTCCGGCTCCCGCAATCTCCGACCTTCCTCTCGTCACTAGAAAATCAAGCAACCGCCGCTTCAATCAAGGCCTGCTCACTCAGACTTCGTGGATTTTCCTGACAAACGCTCTCTGCACCAGGTCTCTCTCACGGCCGAACGCCGCCAGAGCTTTTATCAGAGAATTTTCTGTGACAGTGTGGAAGTTGATGGCGATGTGCTCTCGGATTTTGGGGCTGGATAAGAAGGTCACGCAGAGGGAGCTCTTCTACAAGTTGCTCTGCGATTCACCGGATTATTTCTCGTCTCAGTCGCAGGTGAATCGGACTATCCAAGATGTGGTGGCATTGCTGCGCTGCAGCCGTTACAGTCTCGGGATCATGGCATCTAGCCGAGGGCTTGTTGCCGGTCGCTTGCAGTTGCAGGAGCCAAACCAAGATGTTGTGGACTGTGCTGCCTGTGGATTTTCGGGGCATAATATCTCTGGGGACTTGAATTCCTTGGAGAAGTTGATGATGAAAACGGATGCGAGGTACATCATTGTGGTGGAGAAGCATGCAATTTTCCAGCGGTTGGTCGAGGACCGCGTGTTCAACCAAATTCCCAGCATTCTGATCACTGCCAGAGGGTACCCGGATATAGCAACAAGGATTCTTCTTCACCGGATGAGCAGAGCATTTCCCGACTTGCCAATGTTAGCACTGGTTGACTGGAACCCAGCTGGGCTAGCTATTTTATGCACATTCAAGTTTGGAAGCATAGGGATGGGCCTGGAGGCTTACAGATATGGTAATCTGGTTCCTGTTTTCCTGCTGCTGAATAACAATTTGTTCTTACGTAGAAAGAAACTTACAAGGTCGATCGGTTTATGGCGTGTTTTAGCTTGCAACGTGAAGTGGTTGGGGCTGCGAGGCGAAGACCTAGAGCTCGTACCCGAACAAGCTTTAGTTCCATTGAAACCAAAGGACCTCCAGATTGCCAAGAGCTTGATCTCATCGGAGATCTTGCAGGTTCCGAAAATATCTGGATTGCAGGAGAATTACAGGGCAGAGCTGACATGGATGGTTGAGAGTGGTCAGAGGGCAGAAATTGAAGCTCTGTATTCTCAAGGTTACGATTACCTGGGGAAGTTCATCGCAAGAAAAATTGTGCAAGCCAACTACATCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000228 GO:0000280 GO:0000706 GO:0000729 GO:0003006 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0003916 GO:0003918 GO:0004518 GO:0004519 GO:0004520 GO:0004536 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0007049 GO:0007059 GO:0007127 GO:0007129 GO:0007131 GO:0007275 GO:0008094 GO:0008150 GO:0008152 GO:0009553 GO:0009555 GO:0009791 GO:0009987 GO:0010154 GO:0016043 GO:0016462 GO:0016787 GO:0016788 GO:0016817 GO:0016818 GO:0016853 GO:0016887 GO:0016889 GO:0016894 GO:0017111 GO:0022402 GO:0022414 GO:0022607 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0033554 GO:0034641 GO:0035825 GO:0042138 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044237 GO:0044238 GO:0044260 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045132 GO:0045143 GO:0046483 GO:0048229 GO:0048285 GO:0048316 GO:0048608 GO:0048731 GO:0048856 GO:0050896 GO:0051026 GO:0051276 GO:0051321 GO:0051716 GO:0061458 GO:0061505 GO:0061982 GO:0070013 GO:0070192 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0097159 GO:0098813 GO:0140013 GO:0140097 GO:1901360 GO:1901363 GO:1903046

KEGG Pathways

Metabolic & Signaling

ko04113 map04113

Pfam Domains

Protein Families

TP6A_N

Protein Analysis

427

Amino Acids

48.09

Weight (kDa)

9.3

Isoelectric Point (pI)

48.25

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TP6A_N	PF04406	104 - 164	1.6e-14	Type IIB DNA topoisomerase
TOP6A-Spo11_Toprim	PF21180	216 - 297	6e-24	Topoisomerase 6 subunit A/Spo11, Toprim domain
TOP6A-Spo11_Toprim	PF21180	332 - 420	3.3e-07	Topoisomerase 6 subunit A/Spo11, Toprim domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008447)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G63990 AT1G63990
fragaria_vesca	FvH4_5g03200 FvH4_5g03200 FvH4_5g03200 FvH4_5g03200 FvH4_5g03200
malus_domestica	MD14G1112000.v1.1
prunus_persica	Prupe.5G106600_v2.0.a1
pyrus_communis	pycom14g10670
rosa_chinensis	RchiOBHm_Chr7g0196581
rosa_multiflora	Rmu_co8280957.1_g000001 Rmu_sc0006080.1_g000002 Rmu_sc0040598.1_g000001 Rmu_sc0040599.1_g000001
rosa_roxburghii	Rroxscaffold_3G00259190
rosa_rugosa	Rorug07G0034800 Rorug07G0034900
rosa_samantha	Rh7AG158900 Rh7BG160900 Rh7CG167300 Rh7DG160100
rosa_wichuraiana	Rw7G013890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	418
Acc36I	ACCTGC	2 cut(s)	418, 1111
Acc65I	GGTACC	1 cut(s)	741
AccB1I	GGYRCC	1 cut(s)	741
AccB7I	CCANNNNNTGG	2 cut(s)	451, 685
AccII	CGCG	1 cut(s)	699
AciI	CCGC	7 cut(s)	74, 144, 187, 190, 276, 682, 697
AclWI	GGATC	1 cut(s)	497
AcoI	YGGCCR	1 cut(s)	266
AcsI	RAATTY	4 cut(s)	28, 296, 607, 711
AfaI	GTAC	3 cut(s)	647, 743, 1044
AfiI	CCNNNNNNNGG	2 cut(s)	451, 685
AgsI	TTSAA	7 cut(s)	28, 196, 607, 706, 859, 1069, 1205
AjnI	CCWGG	3 cut(s)	251, 882, 1233
AloI	GAACNNNNNNTCC	2 cut(s)	686, 718
Alw21I	GWGCWC	3 cut(s)	335, 378, 1041
Alw26I	GTCTC	3 cut(s)	261, 423, 488
AlwI	GGATC	1 cut(s)	497
Ama87I	CYCGRG	1 cut(s)	485
AoxI	GGCC	4 cut(s)	81, 202, 266, 880
ApeKI	GCWGC	6 cut(s)	463, 468, 471, 566, 925, 1018
ApoI	RAATTY	4 cut(s)	28, 296, 607, 711
Asp700I	GAANNNNTTC	1 cut(s)	32
Asp718I	GGTACC	1 cut(s)	741
AspLEI	GCGC	1 cut(s)	468
AspS9I	GGNCC	3 cut(s)	694, 880, 1078
AsuC2I	CCSGG	1 cut(s)	746
AsuHPI	GGTGA	3 cut(s)	393, 442, 764
AsuNHI	GCTAGC	1 cut(s)	838
AvaI	CYCGRG	1 cut(s)	485
AvaII	GGWCC	2 cut(s)	694, 1078
BanI	GGYRCC	1 cut(s)	741
BanII	GRGCYC	2 cut(s)	378, 1041
BbsI	GAAGAC	2 cut(s)	12, 1035
Bbv12I	GWGCWC	3 cut(s)	335, 378, 1041
BbvI	GCAGC	6 cut(s)	450, 455, 483, 553, 912, 1005
BccI	CCATC	3 cut(s)	316, 871, 1168
BceAI	ACGGC	3 cut(s)	89, 281, 459
BcgI	CGANNNNNNTGC	2 cut(s)	416, 450
BciT130I	CCWGG	3 cut(s)	253, 884, 1235
BclI	TGATCA	1 cut(s)	726
BcnI	CCSGG	1 cut(s)	746
BcoDI	GTCTC	3 cut(s)	261, 423, 488
BfaI	CTAG	4 cut(s)	171, 501, 839, 1034
BfmI	CTRYAG	1 cut(s)	469
BfuAI	ACCTGC	2 cut(s)	418, 1111
BglII	AGATCT	1 cut(s)	1113
BisI	GCNGC	8 cut(s)	190, 276, 464, 469, 472, 567, 926, 1019
BlsI	GCNGC	8 cut(s)	191, 277, 465, 470, 473, 568, 927, 1020
Bme1390I	CCNGG	4 cut(s)	253, 746, 884, 1235
Bme18I	GGWCC	2 cut(s)	694, 1078
BmeT110I	CYCGRG	1 cut(s)	485
BmgT120I	GGNCC	3 cut(s)	694, 880, 1078
BmiI	GGNNCC	6 cut(s)	141, 540, 743, 827, 912, 1125
BmrFI	CCNGG	4 cut(s)	253, 746, 884, 1235
BmsI	GCATC	2 cut(s)	506, 628
BmtI	GCTAGC	1 cut(s)	842
BpiI	GAAGAC	2 cut(s)	12, 1035
BpmI	CTGGAG	2 cut(s)	905, 1067
BpuEI	CTTGAG	1 cut(s)	1203
BpuMI	CCSGG	1 cut(s)	746
BsaAI	YACGTR	1 cut(s)	950
BsaI	GGTCTC	1 cut(s)	261
BsaJI	CCNNGG	3 cut(s)	505, 612, 1234
BsaWI	WCCGGW	2 cut(s)	403, 774
Bsc4I	CCNNNNNNNGG	2 cut(s)	451, 685
Bse118I	RCCGGY	1 cut(s)	518
Bse1I	ACTGG	2 cut(s)	819, 827
Bse3DI	GCAATG	2 cut(s)	84, 458
BseBI	CCWGG	3 cut(s)	253, 884, 1235
BseDI	CCNNGG	3 cut(s)	505, 612, 1234
BseGI	GGATG	5 cut(s)	63, 643, 783, 882, 1179
BseLI	CCNNNNNNNGG	2 cut(s)	451, 685
BseMI	GCAATG	2 cut(s)	84, 458
BseMII	CTCAG	2 cut(s)	227, 434
BseNI	ACTGG	2 cut(s)	819, 827
BseX3I	CGGCCG	1 cut(s)	266
BseXI	GCAGC	6 cut(s)	450, 455, 483, 553, 912, 1005
BseYI	CCCAGC	3 cut(s)	716, 829, 833
BsgI	GTGCAG	1 cut(s)	232
Bsh1236I	CGCG	1 cut(s)	699
Bsh1285I	CGRYCG	3 cut(s)	269, 523, 975
BshFI	GGCC	4 cut(s)	83, 204, 268, 882
BshNI	GGYRCC	1 cut(s)	741
BsiEI	CGRYCG	3 cut(s)	269, 523, 975
BsiHKAI	GWGCWC	3 cut(s)	335, 378, 1041
BsiHKCI	CYCGRG	1 cut(s)	485
BsiSI	CCGG	6 cut(s)	77, 137, 404, 519, 746, 775
BslFI	GGGAC	1 cut(s)	614
BslI	CCNNNNNNNGG	2 cut(s)	451, 685
BsmAI	GTCTC	3 cut(s)	261, 423, 488
BsmBI	CGTCTC	1 cut(s)	423
BsmFI	GGGAC	1 cut(s)	614
BsmI	GAATGC	1 cut(s)	720
BsnI	GGCC	4 cut(s)	83, 204, 268, 882
Bso31I	GGTCTC	1 cut(s)	261
BsoBI	CYCGRG	1 cut(s)	485
Bsp1286I	GDGCHC	3 cut(s)	335, 378, 1041
Bsp143I	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
BspACI	CCGC	7 cut(s)	74, 144, 187, 190, 276, 682, 697
BspANI	GGCC	4 cut(s)	83, 204, 268, 882
BspCNI	CTCAG	2 cut(s)	226, 433
BspFNI	CGCG	1 cut(s)	699
BspLI	GGNNCC	6 cut(s)	141, 540, 743, 827, 912, 1125
BspMAI	CTGCAG	1 cut(s)	473
BspMI	ACCTGC	2 cut(s)	418, 1111
BspOI	GCTAGC	1 cut(s)	842
BspPI	GGATC	1 cut(s)	497
BspQI	GCTCTTC	1 cut(s)	383
BspT107I	GGYRCC	1 cut(s)	741
BspTNI	GGTCTC	1 cut(s)	261
BsrDI	GCAATG	2 cut(s)	84, 458
BsrFI	RCCGGY	1 cut(s)	518
BsrI	ACTGG	2 cut(s)	819, 827
BssAI	RCCGGY	1 cut(s)	518
BssECI	CCNNGG	3 cut(s)	505, 612, 1234
BssMI	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
BssT1I	CCWWGG	1 cut(s)	612
Bst2UI	CCWGG	3 cut(s)	253, 884, 1235
Bst4CI	ACNGT	3 cut(s)	310, 482, 563
Bst6I	CTCTTC	1 cut(s)	383
BstBAI	YACGTR	1 cut(s)	950
BstC8I	GCNNGC	6 cut(s)	206, 527, 669, 840, 997, 1266
BstDEI	CTNAG	2 cut(s)	213, 420
BstF5I	GGATG	5 cut(s)	63, 643, 783, 882, 1179
BstFNI	CGCG	1 cut(s)	699
BstHHI	GCGC	1 cut(s)	468
BstKTI	GATC	6 cut(s)	43, 492, 729, 975, 1104, 1116
BstMAI	GTCTC	3 cut(s)	261, 423, 488
BstMBI	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
BstMCI	CGRYCG	3 cut(s)	269, 523, 975
BstMWI	GCNNNNNNNGC	4 cut(s)	189, 281, 373, 839
BstNI	CCWGG	3 cut(s)	253, 884, 1235
BstNSI	RCATGY	1 cut(s)	671
BstSCI	CCNGG	4 cut(s)	251, 744, 882, 1233
BstSFI	CTRYAG	1 cut(s)	469
BstSNI	TACGTA	1 cut(s)	950
BstUI	CGCG	1 cut(s)	699
BstV1I	GCAGC	6 cut(s)	450, 455, 483, 553, 912, 1005
BstV2I	GAAGAC	2 cut(s)	12, 1035
BstX2I	RGATCY	1 cut(s)	1113
BstYI	RGATCY	1 cut(s)	1113
BstZI	CGGCCG	1 cut(s)	266
BsuRI	GGCC	4 cut(s)	83, 204, 268, 882
BtgZI	GCGATG	2 cut(s)	341, 1231
BtsCI	GGATG	5 cut(s)	63, 643, 783, 882, 1179
BtsI	GCAGTG	1 cut(s)	729
BtsIMutI	CAGTG	3 cut(s)	315, 729, 812
BveI	ACCTGC	2 cut(s)	418, 1111
Cac8I	GCNNGC	6 cut(s)	206, 527, 669, 840, 997, 1266
CfoI	GCGC	1 cut(s)	468
Cfr10I	RCCGGY	1 cut(s)	518
Cfr13I	GGNCC	3 cut(s)	694, 880, 1078
CsiI	ACCWGGT	1 cut(s)	251
Csp6I	GTAC	3 cut(s)	646, 742, 1043
CviAII	CATG	3 cut(s)	493, 668, 1170
CviQI	GTAC	3 cut(s)	646, 742, 1043
DdeI	CTNAG	2 cut(s)	213, 420
DpnI	GATC	6 cut(s)	42, 491, 728, 974, 1103, 1115
DpnII	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
EaeI	YGGCCR	1 cut(s)	266
EagI	CGGCCG	1 cut(s)	266
Eam1104I	CTCTTC	1 cut(s)	383
EarI	CTCTTC	1 cut(s)	383
EciI	GGCGGA	1 cut(s)	63
Ecl136II	GAGCTC	2 cut(s)	376, 1039
EclXI	CGGCCG	1 cut(s)	266
Eco105I	TACGTA	1 cut(s)	950
Eco130I	CCWWGG	1 cut(s)	612
Eco147I	AGGCCT	1 cut(s)	204
Eco24I	GRGCYC	2 cut(s)	378, 1041
Eco31I	GGTCTC	1 cut(s)	261
Eco47I	GGWCC	2 cut(s)	694, 1078
Eco52I	CGGCCG	1 cut(s)	266
Eco53kI	GAGCTC	2 cut(s)	376, 1039
Eco88I	CYCGRG	1 cut(s)	485
EcoICRI	GAGCTC	2 cut(s)	376, 1039
EcoO109I	RGGNCCY	1 cut(s)	1078
EcoRI	GAATTC	1 cut(s)	607
EcoRII	CCWGG	3 cut(s)	251, 882, 1233
EcoT14I	CCWWGG	1 cut(s)	612
EcoT38I	GRGCYC	2 cut(s)	378, 1041
ErhI	CCWWGG	1 cut(s)	612
Esp3I	CGTCTC	1 cut(s)	423
FaeI	CATG	3 cut(s)	496, 671, 1173
FaiI	YATR	9 cut(s)	494, 588, 669, 752, 850, 872, 900, 982, 1171
FaqI	GGGAC	1 cut(s)	614
FatI	CATG	3 cut(s)	492, 667, 1169
FauI	CCCGC	1 cut(s)	151
FbaI	TGATCA	1 cut(s)	726
Fnu4HI	GCNGC	8 cut(s)	190, 276, 464, 469, 472, 567, 926, 1019
FokI	GGATG	5 cut(s)	50, 650, 790, 889, 1186
FriOI	GRGCYC	2 cut(s)	378, 1041
Fsp4HI	GCNGC	8 cut(s)	190, 276, 464, 469, 472, 567, 926, 1019
FspBI	CTAG	4 cut(s)	171, 501, 839, 1034
GlaI	GCGC	1 cut(s)	467
GluI	GCNGC	8 cut(s)	190, 276, 464, 469, 472, 567, 926, 1019
GsaI	CCCAGC	3 cut(s)	720, 833, 837
GsuI	CTGGAG	2 cut(s)	905, 1067
HaeIII	GGCC	4 cut(s)	83, 204, 268, 882
HapII	CCGG	6 cut(s)	77, 137, 404, 519, 746, 775
HhaI	GCGC	1 cut(s)	468
Hin1II	CATG	3 cut(s)	496, 671, 1173
Hin6I	GCGC	1 cut(s)	466
HinP1I	GCGC	1 cut(s)	466
HincII	GTYRAC	1 cut(s)	820
HindII	GTYRAC	1 cut(s)	820
HindIII	AAGCTT	1 cut(s)	1053
HinfI	GANTC	5 cut(s)	93, 123, 398, 433, 763
HpaII	CCGG	6 cut(s)	77, 137, 404, 519, 746, 775
HphI	GGTGA	3 cut(s)	393, 442, 764
Hpy166II	GTNNAC	2 cut(s)	559, 820
Hpy188III	TCNNGA	6 cut(s)	127, 232, 487, 794, 1084, 1137
Hpy8I	GTNNAC	2 cut(s)	559, 820
HpyAV	CCTTC	2 cut(s)	167, 352
HpyCH4III	ACNGT	3 cut(s)	310, 482, 563
HpyCH4IV	ACGT	2 cut(s)	949, 1002
HpyF10VI	GCNNNNNNNGC	4 cut(s)	189, 281, 373, 839
HpyF3I	CTNAG	2 cut(s)	213, 420
HpySE526I	ACGT	2 cut(s)	949, 1002
Hsp92II	CATG	3 cut(s)	496, 671, 1173
HspAI	GCGC	1 cut(s)	466
KpnI	GGTACC	1 cut(s)	745
Ksp22I	TGATCA	1 cut(s)	726
Kzo9I	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
LguI	GCTCTTC	1 cut(s)	383
LmnI	GCTCC	4 cut(s)	76, 145, 373, 538
Lsp1109I	GCAGC	6 cut(s)	450, 455, 483, 553, 912, 1005
LweI	GCATC	2 cut(s)	506, 628
MabI	ACCWGGT	1 cut(s)	251
MaeI	CTAG	4 cut(s)	171, 501, 839, 1034
MaeII	ACGT	2 cut(s)	949, 1002
MaeIII	GTNAC	5 cut(s)	166, 304, 361, 476, 1223
MalI	GATC	6 cut(s)	42, 491, 728, 974, 1103, 1115
MboI	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
MboII	GAAGA	6 cut(s)	17, 25, 370, 758, 761, 1040
MflI	RGATCY	1 cut(s)	1113
MhlI	GDGCHC	3 cut(s)	335, 378, 1041
MluCI	AATT	9 cut(s)	28, 296, 607, 672, 711, 937, 1150, 1200, 1257
MmeI	TCCRAC	1 cut(s)	177
MroXI	GAANNNNTTC	1 cut(s)	32
MslI	CAYNNNNRTG	3 cut(s)	653, 656, 875
MspA1I	CMGCKG	2 cut(s)	682, 833
MspI	CCGG	6 cut(s)	77, 137, 404, 519, 746, 775
MspR9I	CCNGG	4 cut(s)	253, 746, 884, 1235
Mva1269I	GAATGC	1 cut(s)	720
MvaI	CCWGG	3 cut(s)	253, 884, 1235
MvnI	CGCG	1 cut(s)	699
MwoI	GCNNNNNNNGC	4 cut(s)	189, 281, 373, 839
NciI	CCSGG	1 cut(s)	746
NdeII	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
NheI	GCTAGC	1 cut(s)	838
NlaIII	CATG	3 cut(s)	496, 671, 1173
NlaIV	GGNNCC	6 cut(s)	141, 540, 743, 827, 912, 1125
NmeAIII	GCCGAG	1 cut(s)	530
NmuCI	GTSAC	3 cut(s)	166, 304, 361
NspI	RCATGY	1 cut(s)	671
PaeI	GCATGC	1 cut(s)	671
PaqCI	CACCTGC	1 cut(s)	418
PceI	AGGCCT	1 cut(s)	204
PciSI	GCTCTTC	1 cut(s)	383
PcsI	WCGNNNNNNNCGW	1 cut(s)	696
PctI	GAATGC	1 cut(s)	720
PdmI	GAANNNNTTC	1 cut(s)	32
PfeI	GAWTC	5 cut(s)	93, 123, 398, 433, 763
PflMI	CCANNNNNTGG	2 cut(s)	451, 685
PkrI	GCNGC	8 cut(s)	191, 277, 465, 470, 473, 568, 927, 1020
Ple19I	CGATCG	1 cut(s)	975
Ppu21I	YACGTR	1 cut(s)	950
PpuMI	RGGWCCY	1 cut(s)	1078
Psp124BI	GAGCTC	2 cut(s)	378, 1041
Psp5II	RGGWCCY	1 cut(s)	1078
Psp6I	CCWGG	3 cut(s)	251, 882, 1233
PspFI	CCCAGC	3 cut(s)	716, 829, 833
PspGI	CCWGG	3 cut(s)	251, 882, 1233
PspN4I	GGNNCC	6 cut(s)	141, 540, 743, 827, 912, 1125
PspPI	GGNCC	3 cut(s)	694, 880, 1078
PspPPI	RGGWCCY	1 cut(s)	1078
PstI	CTGCAG	1 cut(s)	473
PsuI	RGATCY	1 cut(s)	1113
PvuI	CGATCG	1 cut(s)	975
PvuII	CAGCTG	1 cut(s)	833
RsaI	GTAC	3 cut(s)	647, 743, 1044
RsaNI	GTAC	3 cut(s)	646, 742, 1043
RseI	CAYNNNNRTG	3 cut(s)	653, 656, 875
SacI	GAGCTC	2 cut(s)	378, 1041
SapI	GCTCTTC	1 cut(s)	383
SatI	GCNGC	8 cut(s)	190, 276, 464, 469, 472, 567, 926, 1019
Sau3AI	GATC	6 cut(s)	40, 489, 726, 972, 1101, 1113
Sau96I	GGNCC	3 cut(s)	694, 880, 1078
ScrFI	CCNGG	4 cut(s)	253, 746, 884, 1235
SduI	GDGCHC	3 cut(s)	335, 378, 1041
SexAI	ACCWGGT	1 cut(s)	251
SfaNI	GCATC	2 cut(s)	506, 628
SfcI	CTRYAG	1 cut(s)	469
SinI	GGWCC	2 cut(s)	694, 1078
SmiMI	CAYNNNNRTG	3 cut(s)	653, 656, 875
SmlI	CTYRAG	1 cut(s)	1218
SmoI	CTYRAG	1 cut(s)	1218
SnaBI	TACGTA	1 cut(s)	950
SphI	GCATGC	1 cut(s)	671
Sse9I	AATT	9 cut(s)	28, 296, 607, 672, 711, 937, 1150, 1200, 1257
SseBI	AGGCCT	1 cut(s)	204
SsiI	CCGC	7 cut(s)	74, 144, 187, 190, 276, 682, 697
SspMI	CTAG	4 cut(s)	171, 501, 839, 1034
SstI	GAGCTC	2 cut(s)	378, 1041
StuI	AGGCCT	1 cut(s)	204
StyD4I	CCNGG	4 cut(s)	251, 744, 882, 1233
StyI	CCWWGG	1 cut(s)	612
TaaI	ACNGT	3 cut(s)	310, 482, 563
TaiI	ACGT	2 cut(s)	952, 1005
TaqI	TCGA	2 cut(s)	690, 971
TasI	AATT	9 cut(s)	28, 296, 607, 672, 711, 937, 1150, 1200, 1257
TauI	GCSGC	2 cut(s)	192, 278
TfiI	GAWTC	5 cut(s)	93, 123, 398, 433, 763
TscAI	CASTG	3 cut(s)	315, 736, 819
TseFI	GTSAC	3 cut(s)	166, 304, 361
TseI	GCWGC	6 cut(s)	463, 468, 471, 566, 925, 1018
Tsp45I	GTSAC	3 cut(s)	166, 304, 361
TspDTI	ATGAA	2 cut(s)	644, 1234
TspGWI	ACGGA	1 cut(s)	650
TspRI	CASTG	3 cut(s)	315, 736, 819
Van91I	CCANNNNNTGG	2 cut(s)	451, 685
VpaK11BI	GGWCC	2 cut(s)	694, 1078
XapI	RAATTY	4 cut(s)	28, 296, 607, 711
XceI	RCATGY	1 cut(s)	671
XmnI	GAANNNNTTC	1 cut(s)	32
XspI	CTAG	4 cut(s)	171, 501, 839, 1034

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.