AT1G64000
WRKY Family

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0008521
Physical Location & Seq
Forward (+)
23747304 .. 23749053
1750 bp
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UTR
Exon/CDS
Intron
AT1G64000.1

Sequence Viewer

Length: 588 bp
ATGGAAGGGGTTGACAACACAAATCCTATGTTAACCCTAGAAGAAGGCGAAAACAACAATCCTTTTTCTTCCTTAGATGACAAAACATTAATGATGATGGCTCCTTCGTTAATCTTTTCGGGCGATGTAGGTCCATCTTCTTCTTCTTGTACTCCAGCAGGTTATCATCTATCTGCTCAGCTGGAGAACTTTCGAGGAGGTGGAGGAGAGATGGGAGGATTAGTGAGTAATAATAGCAATAATAGTGATCATAATAAGAATTGCAACAAAGGAAAAGGGAAGAGAACTTTGGCAATGCAGAGGATAGCTTTTCATACAAGGAGTGATGATGATGTTCTTGATGATGGTTATCGTTGGCGAAAGTACGGTCAGAAATCTGTCAAGAACAATGCTCATCCCAGGAGCTATTATAGATGTACATACCACACATGCAACGTGAAGAAACAAGTGCAAAGACTGGCAAAAGATCCAAACGTTGTCGTAACAACCTACGAAGGTGTTCATAATCATCCTTGTGAGAAGCTCATGGAGACTCTTAGCCCTCTCCTTAGGCAACTTCAGTTCCTCTCAAGAGTTTCTGATCTGTAA

Protein Analysis

195

Amino Acids

21.77

Weight (kDa)

8.25

Isoelectric Point (pI)

36.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 114 - 171 9.8e-27 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 149
AclI AACGTT 1 cut(s) 474
AclWI GGATC 1 cut(s) 461
AcuI CTGAAG 1 cut(s) 542
AfaI GTAC 3 cut(s) 151, 365, 418
AjnI CCWGG 1 cut(s) 398
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 4 cut(s) 181, 308, 405, 523
AluI AGCT 4 cut(s) 181, 308, 405, 523
Alw26I GTCTC 1 cut(s) 524
AlwI GGATC 1 cut(s) 461
ArsI GACNNNNNNTTYG 2 cut(s) 352, 384
AseI ATTAAT 1 cut(s) 89
Asp700I GAANNNNTTC 1 cut(s) 498
AspS9I GGNCC 1 cut(s) 131
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 548
BccI CCATC 4 cut(s) 91, 142, 205, 338
BciT130I CCWGG 1 cut(s) 400
BclI TGATCA 1 cut(s) 247
BcoDI GTCTC 1 cut(s) 524
BfaI CTAG 1 cut(s) 38
BfuAI ACCTGC 1 cut(s) 149
BlpI GCTNAGC 1 cut(s) 177
Bme1390I CCNGG 1 cut(s) 400
Bme18I GGWCC 1 cut(s) 131
BmgT120I GGNCC 1 cut(s) 131
BmiI GGNNCC 1 cut(s) 102
BmrFI CCNGG 1 cut(s) 400
BpmI CTGGAG 2 cut(s) 138, 203
Bpu1102I GCTNAGC 1 cut(s) 177
BpuEI CTTGAG 1 cut(s) 553
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 1 cut(s) 398
BsaXI ACNNNNNCTCC 2 cut(s) 207, 237
Bse1I ACTGG 1 cut(s) 462
Bse21I CCTNAGG 1 cut(s) 548
Bse3DI GCAATG 1 cut(s) 300
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 400
BseDI CCNNGG 1 cut(s) 398
BseGI GGATG 2 cut(s) 394, 508
BseJI GATNNNNATC 1 cut(s) 348
BseMI GCAATG 1 cut(s) 300
BseMII CTCAG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 462
BseRI GAGGAG 2 cut(s) 210, 219
BsmAI GTCTC 1 cut(s) 524
Bsp1407I TGTACA 1 cut(s) 416
Bsp143I GATC 3 cut(s) 247, 466, 580
Bsp1720I GCTNAGC 1 cut(s) 177
BspCNI CTCAG 1 cut(s) 190
BspLI GGNNCC 1 cut(s) 102
BspMI ACCTGC 1 cut(s) 149
BspPI GGATC 1 cut(s) 461
BsrDI GCAATG 1 cut(s) 300
BsrGI TGTACA 1 cut(s) 416
BsrI ACTGG 1 cut(s) 462
BssECI CCNNGG 1 cut(s) 398
BssMI GATC 3 cut(s) 247, 466, 580
Bst2UI CCWGG 1 cut(s) 400
Bst4CI ACNGT 1 cut(s) 368
Bst6I CTCTTC 1 cut(s) 275
BstAUI TGTACA 1 cut(s) 416
BstDEI CTNAG 4 cut(s) 73, 177, 536, 548
BstF5I GGATG 2 cut(s) 394, 508
BstKTI GATC 3 cut(s) 250, 469, 583
BstMAI GTCTC 1 cut(s) 524
BstMBI GATC 3 cut(s) 247, 466, 580
BstNI CCWGG 1 cut(s) 400
BstNSI RCATGY 1 cut(s) 432
BstSCI CCNGG 1 cut(s) 398
BstX2I RGATCY 1 cut(s) 466
BstYI RGATCY 1 cut(s) 466
Bsu36I CCTNAGG 1 cut(s) 548
BtgZI GCGATG 1 cut(s) 138
BtsCI GGATG 2 cut(s) 394, 508
BveI ACCTGC 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 3 cut(s) 150, 364, 417
CviAII CATG 2 cut(s) 429, 526
CviJI RGCY 6 cut(s) 101, 181, 308, 405, 523, 540
CviKI_1 RGCY 6 cut(s) 101, 181, 308, 405, 523, 540
CviQI GTAC 3 cut(s) 150, 364, 417
DdeI CTNAG 4 cut(s) 73, 177, 536, 548
DpnI GATC 3 cut(s) 249, 468, 582
DpnII GATC 3 cut(s) 247, 466, 580
Eam1104I CTCTTC 1 cut(s) 275
EarI CTCTTC 1 cut(s) 275
Eco47I GGWCC 1 cut(s) 131
Eco57I CTGAAG 1 cut(s) 542
Eco81I CCTNAGG 1 cut(s) 548
EcoRII CCWGG 1 cut(s) 398
FaeI CATG 2 cut(s) 432, 529
FaiI YATR 8 cut(s) 29, 252, 315, 411, 421, 430, 504, 527
FatI CATG 2 cut(s) 428, 525
FbaI TGATCA 1 cut(s) 247
FokI GGATG 2 cut(s) 381, 495
FspBI CTAG 1 cut(s) 38
GsuI CTGGAG 2 cut(s) 138, 203
Hin1II CATG 2 cut(s) 432, 529
HincII GTYRAC 2 cut(s) 13, 33
HindII GTYRAC 2 cut(s) 13, 33
HinfI GANTC 1 cut(s) 532
HpaI GTTAAC 1 cut(s) 33
Hpy166II GTNNAC 2 cut(s) 13, 33
Hpy188I TCNGA 2 cut(s) 372, 580
Hpy188III TCNNGA 3 cut(s) 338, 382, 570
Hpy8I GTNNAC 2 cut(s) 13, 33
HpyAV CCTTC 3 cut(s) 38, 114, 488
HpyCH4III ACNGT 1 cut(s) 368
HpyCH4IV ACGT 2 cut(s) 435, 474
HpyCH4V TGCA 4 cut(s) 264, 298, 432, 451
HpyF3I CTNAG 4 cut(s) 73, 177, 536, 548
HpySE526I ACGT 2 cut(s) 435, 474
Hsp92II CATG 2 cut(s) 432, 529
Ksp22I TGATCA 1 cut(s) 247
KspAI GTTAAC 1 cut(s) 33
Kzo9I GATC 3 cut(s) 247, 466, 580
LmnI GCTCC 2 cut(s) 106, 402
LpnPI CCDG 6 cut(s) 144, 167, 168, 385, 412, 443
MaeI CTAG 1 cut(s) 38
MaeII ACGT 2 cut(s) 435, 474
MaeIII GTNAC 1 cut(s) 481
MalI GATC 3 cut(s) 249, 468, 582
MboI GATC 3 cut(s) 247, 466, 580
MboII GAAGA 7 cut(s) 53, 60, 129, 132, 135, 292, 451
MflI RGATCY 1 cut(s) 466
MluCI AATT 1 cut(s) 259
MlyI GAGTC 1 cut(s) 526
MnlI CCTC 7 cut(s) 188, 191, 197, 209, 294, 552, 575
MroXI GAANNNNTTC 1 cut(s) 498
MseI TTAA 3 cut(s) 32, 89, 110
MslI CAYNNNNRTG 1 cut(s) 513
MspA1I CMGCKG 1 cut(s) 181
MspR9I CCNGG 1 cut(s) 400
MvaI CCWGG 1 cut(s) 400
NdeII GATC 3 cut(s) 247, 466, 580
NlaIII CATG 2 cut(s) 432, 529
NlaIV GGNNCC 1 cut(s) 102
NspI RCATGY 1 cut(s) 432
PdmI GAANNNNTTC 1 cut(s) 498
PleI GAGTC 1 cut(s) 526
PpsI GAGTC 1 cut(s) 526
PshBI ATTAAT 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 474
Psp6I CCWGG 1 cut(s) 398
PspGI CCWGG 1 cut(s) 398
PspN4I GGNNCC 1 cut(s) 102
PspPI GGNCC 1 cut(s) 131
PsuI RGATCY 1 cut(s) 466
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 3 cut(s) 151, 365, 418
RsaNI GTAC 3 cut(s) 150, 364, 417
RseI CAYNNNNRTG 1 cut(s) 513
SaqAI TTAA 3 cut(s) 32, 89, 110
Sau3AI GATC 3 cut(s) 247, 466, 580
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 1 cut(s) 526
ScrFI CCNGG 1 cut(s) 400
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 513
SmlI CTYRAG 1 cut(s) 568
SmoI CTYRAG 1 cut(s) 568
Sse9I AATT 1 cut(s) 259
SspMI CTAG 1 cut(s) 38
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 1 cut(s) 368
TaiI ACGT 2 cut(s) 438, 477
TaqI TCGA 1 cut(s) 193
TasI AATT 1 cut(s) 259
TatI WGTACW 2 cut(s) 149, 416
Tru1I TTAA 3 cut(s) 32, 89, 110
Tru9I TTAA 3 cut(s) 32, 89, 110
TspDTI ATGAA 2 cut(s) 302, 491
VpaK11BI GGWCC 1 cut(s) 131
VspI ATTAAT 1 cut(s) 89
XceI RCATGY 1 cut(s) 432
XmnI GAANNNNTTC 1 cut(s) 498
XspI CTAG 1 cut(s) 38
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.