Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0008521

Physical Location & Seq

Genomic Coordinates Reverse (-)

23690319 .. 23691083

Gene Structure 765 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1123900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 627 bp

ATGGAAAACCAATATTATCCATCTCCTGCTGCAGCAACATCATCATCCTCACCAACATATCAATTCCCACCCCAAAATAACCTCTTTACACCCTCATCATCATCTCTCTCCATCAACCTTTTCGACAACCCTCATTCTCCTCCAGATCCTCATCATCACCAAGTCCAGATTCCACCTGACATTGATTGGATCAGCCTCCTCTCTGGTGATCAACCTGCAGGTGACCTACTTACTACTCATAATAATCATGATCAAGCGGGTGATCGTGCGGAAGAAATAAGGGGTTCGAATAGAAGGAAAGGCGGTGGGAGTAGTAGGAATTATACAAGAAGATCGAGCCGGCCTAGGGTTGCATTCCATACTCGAAGCACGGATTATATTCTTGATGATGGCTTCCGCTGGAGAAAGTACGGACAGAAAGCCGTCAAGAACACCTTATGTCCCAGGAGTTACTATCGATGCACACATAACACATGCAGTGTGAAGAAGCAGGTCCAGAGGCTATCAAACGACACAAGCATCGTCGTCACAACGTATGAAGGAGTTCACAATCATCCATGTGAAGAACTCATGGAAACCCTAACTCCTCTTCTCAAGCAAATGCAGTTCCTCTCAGCTAGGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003700 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0140110 GO:1903506 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WRKY

Protein Analysis

209

Amino Acids

23.62

Weight (kDa)

8.91

Isoelectric Point (pI)

56.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WRKY	PF03106	129 - 186	2.4e-26	WRKY DNA -binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0008521)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G64000 AT2G46130 AT2G46130 AT5G41570
fragaria_vesca	FvH4_7g15430
malus_domestica	MD01G1071600.v1.1 MD01G1123900.v1.1 MD07G1131000.v1.1
prunus_persica	Prupe.2G177800_v2.0.a1 Prupe.2G231300_v2.0.a1
pyrus_communis	pycom01g10080 pycom01g15040 pycom07g12810
rosa_chinensis	RchiOBHm_Chr1g0357751
rosa_laevigata	RLG00000028000
rosa_multiflora	Rmu_ssc0000399.1_g000021
rosa_roxburghii	Rroxscaffold_4G00298540
rosa_rugosa	Rorug01G0258300
rosa_samantha	Rh1AG272200 Rh1BG239400 Rh1CG256100 Rh1DG267700
rosa_wichuraiana	Rw1G024290

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	209
Acc36I	ACCTGC	3 cut(s)	209, 223, 481
AciI	CCGC	4 cut(s)	257, 269, 303, 397
AclWI	GGATC	2 cut(s)	140, 197
AfaI	GTAC	1 cut(s)	410
AfiI	CCNNNNNNNGG	1 cut(s)	346
AjnI	CCWGG	1 cut(s)	443
AjuI	GAANNNNNNNTTGG	2 cut(s)	153, 185
AluBI	AGCT	1 cut(s)	617
AluI	AGCT	1 cut(s)	617
AlwI	GGATC	2 cut(s)	140, 197
AoxI	GGCC	1 cut(s)	341
ApeKI	GCWGC	2 cut(s)	29, 32
Asp700I	GAANNNNTTC	1 cut(s)	543
AspA2I	CCTAGG	1 cut(s)	344
AspS9I	GGNCC	1 cut(s)	493
AsuHPI	GGTGA	5 cut(s)	42, 149, 218, 233, 272
AsuII	TTCGAA	1 cut(s)	287
AvaII	GGWCC	1 cut(s)	493
AvrII	CCTAGG	1 cut(s)	344
BbvI	GCAGC	2 cut(s)	16, 44
BccI	CCATC	3 cut(s)	28, 119, 383
BceAI	ACGGC	1 cut(s)	407
BciT130I	CCWGG	1 cut(s)	445
BclI	TGATCA	2 cut(s)	208, 250
BfaI	CTAG	2 cut(s)	345, 618
BfmI	CTRYAG	2 cut(s)	30, 216
BfuAI	ACCTGC	3 cut(s)	209, 223, 481
BisI	GCNGC	2 cut(s)	30, 33
BlnI	CCTAGG	1 cut(s)	344
BlsI	GCNGC	2 cut(s)	31, 34
Bme1390I	CCNGG	1 cut(s)	445
Bme18I	GGWCC	1 cut(s)	493
BmgT120I	GGNCC	1 cut(s)	493
BmrFI	CCNGG	1 cut(s)	445
BmsI	GCATC	2 cut(s)	449, 528
BpmI	CTGGAG	2 cut(s)	126, 421
Bpu14I	TTCGAA	1 cut(s)	287
BpuEI	CTTGAG	1 cut(s)	578
Bsa29I	ATCGAT	1 cut(s)	457
BsaBI	GATNNNNATC	1 cut(s)	150
BsaJI	CCNNGG	2 cut(s)	344, 443
BsaXI	ACNNNNNCTCC	2 cut(s)	568, 598
Bsc4I	CCNNNNNNNGG	1 cut(s)	346
Bse118I	RCCGGY	1 cut(s)	339
Bse8I	GATNNNNATC	1 cut(s)	150
BseBI	CCWGG	1 cut(s)	445
BseCI	ATCGAT	1 cut(s)	457
BseDI	CCNNGG	2 cut(s)	344, 443
BseGI	GGATG	2 cut(s)	44, 553
BseJI	GATNNNNATC	1 cut(s)	150
BseLI	CCNNNNNNNGG	1 cut(s)	346
BseMII	CTCAG	1 cut(s)	627
BseRI	GAGGAG	3 cut(s)	129, 188, 576
BseXI	GCAGC	2 cut(s)	16, 44
BshFI	GGCC	1 cut(s)	343
BshVI	ATCGAT	1 cut(s)	457
BsiSI	CCGG	1 cut(s)	340
BslFI	GGGAC	1 cut(s)	426
BslI	CCNNNNNNNGG	1 cut(s)	346
BsmFI	GGGAC	1 cut(s)	426
BsmI	GAATGC	1 cut(s)	353
BsnI	GGCC	1 cut(s)	343
Bsp119I	TTCGAA	1 cut(s)	287
Bsp143I	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
BspACI	CCGC	4 cut(s)	257, 269, 303, 397
BspANI	GGCC	1 cut(s)	343
BspCNI	CTCAG	1 cut(s)	626
BspDI	ATCGAT	1 cut(s)	457
BspHI	TCATGA	1 cut(s)	247
BspMAI	CTGCAG	2 cut(s)	34, 220
BspMI	ACCTGC	3 cut(s)	209, 223, 481
BspPI	GGATC	2 cut(s)	140, 197
BspT104I	TTCGAA	1 cut(s)	287
BsrFI	RCCGGY	1 cut(s)	339
BssAI	RCCGGY	1 cut(s)	339
BssECI	CCNNGG	2 cut(s)	344, 443
BssMI	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
BssT1I	CCWWGG	1 cut(s)	344
Bst2UI	CCWGG	1 cut(s)	445
Bst6I	CTCTTC	1 cut(s)	594
BstBI	TTCGAA	1 cut(s)	287
BstC8I	GCNNGC	1 cut(s)	341
BstDEI	CTNAG	1 cut(s)	613
BstEII	GGTNACC	1 cut(s)	221
BstF5I	GGATG	2 cut(s)	44, 553
BstKTI	GATC	6 cut(s)	148, 192, 211, 253, 265, 335
BstMBI	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
BstNI	CCWGG	1 cut(s)	445
BstNSI	RCATGY	1 cut(s)	477
BstPI	GGTNACC	1 cut(s)	221
BstSCI	CCNGG	1 cut(s)	443
BstSFI	CTRYAG	2 cut(s)	30, 216
BstV1I	GCAGC	2 cut(s)	16, 44
BstX2I	RGATCY	1 cut(s)	145
BstYI	RGATCY	1 cut(s)	145
Bsu15I	ATCGAT	1 cut(s)	457
BsuRI	GGCC	1 cut(s)	343
BsuTUI	ATCGAT	1 cut(s)	457
BtsCI	GGATG	2 cut(s)	44, 553
BtsI	GCAGTG	1 cut(s)	484
BtsIMutI	CAGTG	1 cut(s)	484
BveI	ACCTGC	3 cut(s)	209, 223, 481
Cac8I	GCNNGC	1 cut(s)	341
CciI	TCATGA	1 cut(s)	247
Cfr10I	RCCGGY	1 cut(s)	339
Cfr13I	GGNCC	1 cut(s)	493
ClaI	ATCGAT	1 cut(s)	457
Csp6I	GTAC	1 cut(s)	409
CviAII	CATG	4 cut(s)	248, 474, 558, 571
CviJI	RGCY	7 cut(s)	195, 339, 343, 393, 422, 502, 617
CviKI_1	RGCY	7 cut(s)	195, 339, 343, 393, 422, 502, 617
CviQI	GTAC	1 cut(s)	409
DdeI	CTNAG	1 cut(s)	613
DpnI	GATC	6 cut(s)	147, 191, 210, 252, 264, 334
DpnII	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
Eam1104I	CTCTTC	1 cut(s)	594
EarI	CTCTTC	1 cut(s)	594
Eco130I	CCWWGG	1 cut(s)	344
Eco47I	GGWCC	1 cut(s)	493
Eco91I	GGTNACC	1 cut(s)	221
EcoO65I	GGTNACC	1 cut(s)	221
EcoRII	CCWGG	1 cut(s)	443
EcoT14I	CCWWGG	1 cut(s)	344
ErhI	CCWWGG	1 cut(s)	344
FaeI	CATG	4 cut(s)	251, 477, 561, 574
FalI	AAGNNNNNCTT	2 cut(s)	419, 451
FaqI	GGGAC	1 cut(s)	426
FatI	CATG	4 cut(s)	247, 473, 557, 570
FauI	CCCGC	1 cut(s)	250
FbaI	TGATCA	2 cut(s)	208, 250
Fnu4HI	GCNGC	2 cut(s)	30, 33
FokI	GGATG	2 cut(s)	31, 540
Fsp4HI	GCNGC	2 cut(s)	30, 33
FspBI	CTAG	2 cut(s)	345, 618
GluI	GCNGC	2 cut(s)	30, 33
GsuI	CTGGAG	2 cut(s)	126, 421
HaeIII	GGCC	1 cut(s)	343
HapII	CCGG	1 cut(s)	340
Hin1II	CATG	4 cut(s)	251, 477, 561, 574
HinfI	GANTC	1 cut(s)	169
HpaII	CCGG	1 cut(s)	340
HphI	GGTGA	5 cut(s)	42, 149, 218, 233, 272
Hpy166II	GTNNAC	1 cut(s)	547
Hpy188III	TCNNGA	6 cut(s)	143, 166, 248, 383, 427, 496
Hpy8I	GTNNAC	1 cut(s)	547
Hpy99I	CGWCG	1 cut(s)	527
HpyAV	CCTTC	2 cut(s)	288, 533
HpyCH4IV	ACGT	1 cut(s)	533
HpyCH4V	TGCA	6 cut(s)	32, 218, 353, 462, 477, 604
HpyF3I	CTNAG	1 cut(s)	613
HpySE526I	ACGT	1 cut(s)	533
Hsp92II	CATG	4 cut(s)	251, 477, 561, 574
KroI	GCCGGC	1 cut(s)	339
KroNI	GCCGGC	1 cut(s)	341
Ksp22I	TGATCA	2 cut(s)	208, 250
Kzo9I	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
Lsp1109I	GCAGC	2 cut(s)	16, 44
LweI	GCATC	2 cut(s)	449, 528
MaeI	CTAG	2 cut(s)	345, 618
MaeII	ACGT	1 cut(s)	533
MaeIII	GTNAC	3 cut(s)	221, 449, 526
MalI	GATC	6 cut(s)	147, 191, 210, 252, 264, 334
MboI	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
MboII	GAAGA	5 cut(s)	284, 342, 496, 575, 581
MflI	RGATCY	1 cut(s)	145
MluCI	AATT	2 cut(s)	62, 319
MroNI	GCCGGC	1 cut(s)	339
MroXI	GAANNNNTTC	1 cut(s)	543
MslI	CAYNNNNRTG	1 cut(s)	558
MspA1I	CMGCKG	1 cut(s)	399
MspI	CCGG	1 cut(s)	340
MspR9I	CCNGG	1 cut(s)	445
Mva1269I	GAATGC	1 cut(s)	353
MvaI	CCWGG	1 cut(s)	445
NaeI	GCCGGC	1 cut(s)	341
NdeII	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
NgoMIV	GCCGGC	1 cut(s)	339
NlaIII	CATG	4 cut(s)	251, 477, 561, 574
NmuCI	GTSAC	2 cut(s)	221, 526
NspI	RCATGY	1 cut(s)	477
NspV	TTCGAA	1 cut(s)	287
PagI	TCATGA	1 cut(s)	247
PaqCI	CACCTGC	1 cut(s)	209
PctI	GAATGC	1 cut(s)	353
PdiI	GCCGGC	1 cut(s)	341
PdmI	GAANNNNTTC	1 cut(s)	543
PfeI	GAWTC	1 cut(s)	169
PkrI	GCNGC	2 cut(s)	31, 34
Psp6I	CCWGG	1 cut(s)	443
PspEI	GGTNACC	1 cut(s)	221
PspGI	CCWGG	1 cut(s)	443
PspPI	GGNCC	1 cut(s)	493
PstI	CTGCAG	2 cut(s)	34, 220
PsuI	RGATCY	1 cut(s)	145
RsaI	GTAC	1 cut(s)	410
RsaNI	GTAC	1 cut(s)	409
RseI	CAYNNNNRTG	1 cut(s)	558
SatI	GCNGC	2 cut(s)	30, 33
Sau3AI	GATC	6 cut(s)	145, 189, 208, 250, 262, 332
Sau96I	GGNCC	1 cut(s)	493
SbfI	CCTGCAGG	1 cut(s)	220
ScrFI	CCNGG	1 cut(s)	445
SdaI	CCTGCAGG	1 cut(s)	220
SfaNI	GCATC	2 cut(s)	449, 528
SfcI	CTRYAG	2 cut(s)	30, 216
SfuI	TTCGAA	1 cut(s)	287
SinI	GGWCC	1 cut(s)	493
SmiMI	CAYNNNNRTG	1 cut(s)	558
SmlI	CTYRAG	1 cut(s)	593
SmoI	CTYRAG	1 cut(s)	593
Sse8387I	CCTGCAGG	1 cut(s)	220
Sse9I	AATT	2 cut(s)	62, 319
SsiI	CCGC	4 cut(s)	257, 269, 303, 397
SspI	AATATT	1 cut(s)	14
SspMI	CTAG	2 cut(s)	345, 618
StyD4I	CCNGG	1 cut(s)	443
StyI	CCWWGG	1 cut(s)	344
TaiI	ACGT	1 cut(s)	536
TaqI	TCGA	5 cut(s)	123, 287, 335, 364, 457
TasI	AATT	2 cut(s)	62, 319
TfiI	GAWTC	1 cut(s)	169
TscAI	CASTG	1 cut(s)	484
TseFI	GTSAC	2 cut(s)	221, 526
TseI	GCWGC	2 cut(s)	29, 32
Tsp45I	GTSAC	2 cut(s)	221, 526
TspDTI	ATGAA	1 cut(s)	552
TspGWI	ACGGA	2 cut(s)	386, 426
TspRI	CASTG	1 cut(s)	484
VpaK11BI	GGWCC	1 cut(s)	493
XceI	RCATGY	1 cut(s)	477
XmaJI	CCTAGG	1 cut(s)	344
XmnI	GAANNNNTTC	1 cut(s)	543
XspI	CTAG	2 cut(s)	345, 618

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.