MD01G1071600.v1.1
WRKY Family

WRKY transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0008521
Physical Location & Seq
Forward (+)
17669166 .. 17671888
2723 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1071600.v1.1.491

Sequence Viewer

Length: 657 bp
ATGGAAGATCATCATCAACAGCATAATCCACCTCCATTAACCTCCCAATCGCTGCCCTTGTCATTACCATTCTTGTTACCGCCTCCACCCCAAAACCCTCGTCCCCCTTCTCCCTATCTCTTCACATCCTCTTCATCCTCCTCATTGATGACTAACCCTCCCGATCTTCTAGACATTGATTGGGTTAGCCTTCTCTCTGGTGGTACTCAAGCGGCAGGCGGGTTTAATCATGATCAGATTAATAGTTTTAAGCCTATGGCTATGATGGACAATAATATTAGTGGCTCTGCTTGGAATACTGGAGCTGATCAGGAAGAAAAGGTTGGTGTTAAAAGGAAGGGTGGGGGTAAAATGAGAAAAGCAGCGGCGAGCCGGCCTAGGTTTGCGTTTCAGACTCGGAGTGAGGATGATATTCTTGATGATGGCTACCGCTGGAGAAAATACGGTCAGAAAGCAGTCAAGAACAGTTCATATCCCAGGAGCTACTACCGCTGCACACATCACACATGCAATGTGAAAAAACAGGTCCAGAGGCTGTCTAAAGACACGAGCATTGTCGTGACAACGTATGAAGGAATCCACAATCATCCTTGCGAGAAACTCATGGAAACCCTAACTCCTCTACTGAAGCAAATGCAATTCCTCTCTAGGTTCTAA

Protein Analysis

219

Amino Acids

24.56

Weight (kDa)

9.36

Isoelectric Point (pI)

48.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WRKY PF03106 140 - 197 1.1e-26 WRKY DNA -binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 80, 212, 219, 365, 430, 490
AcuI CTGAAG 1 cut(s) 647
AfaI GTAC 1 cut(s) 205
AjnI CCWGG 1 cut(s) 476
AjuI GAANNNNNNNTTGG 2 cut(s) 306, 338
AluBI AGCT 2 cut(s) 305, 483
AluI AGCT 2 cut(s) 305, 483
AlwNI CAGNNNCTG 1 cut(s) 535
AoxI GGCC 1 cut(s) 374
ApeKI GCWGC 3 cut(s) 52, 362, 492
ArsI GACNNNNNNTTYG 2 cut(s) 85, 117
AseI ATTAAT 1 cut(s) 240
AspA2I CCTAGG 1 cut(s) 377
AspS9I GGNCC 1 cut(s) 526
AvaII GGWCC 1 cut(s) 526
AvrII CCTAGG 1 cut(s) 377
BauI CACGAG 1 cut(s) 547
BbvI GCAGC 3 cut(s) 39, 374, 479
BccI CCATC 2 cut(s) 259, 416
BciT130I CCWGG 1 cut(s) 478
BclI TGATCA 2 cut(s) 232, 307
BfaI CTAG 3 cut(s) 170, 378, 648
BisI GCNGC 5 cut(s) 53, 213, 363, 366, 493
BlnI CCTAGG 1 cut(s) 377
BlsI GCNGC 5 cut(s) 54, 214, 364, 367, 494
Bme1390I CCNGG 1 cut(s) 478
Bme18I GGWCC 1 cut(s) 526
BmgT120I GGNCC 1 cut(s) 526
BmrFI CCNGG 1 cut(s) 478
BpmI CTGGAG 2 cut(s) 321, 454
BpuEI CTTGAG 1 cut(s) 192
BsaBI GATNNNNATC 1 cut(s) 12
BsaJI CCNNGG 2 cut(s) 377, 476
BsaXI ACNNNNNCTCC 4 cut(s) 142, 172, 601, 631
Bse118I RCCGGY 1 cut(s) 372
Bse1I ACTGG 1 cut(s) 304
Bse3DI GCAATG 1 cut(s) 517
Bse8I GATNNNNATC 1 cut(s) 12
BseBI CCWGG 1 cut(s) 478
BseDI CCNNGG 2 cut(s) 377, 476
BseGI GGATG 4 cut(s) 125, 134, 412, 586
BseJI GATNNNNATC 1 cut(s) 12
BseMI GCAATG 1 cut(s) 517
BseNI ACTGG 1 cut(s) 304
BseRI GAGGAG 2 cut(s) 130, 609
BseXI GCAGC 3 cut(s) 39, 374, 479
BsgI GTGCAG 1 cut(s) 478
BshFI GGCC 1 cut(s) 376
BsiSI CCGG 1 cut(s) 373
BslFI GGGAC 1 cut(s) 87
BsmFI GGGAC 1 cut(s) 87
BsnI GGCC 1 cut(s) 376
Bsp143I GATC 4 cut(s) 7, 163, 232, 307
BspACI CCGC 6 cut(s) 80, 212, 219, 365, 430, 490
BspANI GGCC 1 cut(s) 376
BspHI TCATGA 1 cut(s) 229
BsrDI GCAATG 1 cut(s) 517
BsrFI RCCGGY 1 cut(s) 372
BsrI ACTGG 1 cut(s) 304
BssAI RCCGGY 1 cut(s) 372
BssECI CCNNGG 2 cut(s) 377, 476
BssMI GATC 4 cut(s) 7, 163, 232, 307
BssSI CACGAG 1 cut(s) 547
BssT1I CCWWGG 1 cut(s) 377
Bst2BI CACGAG 1 cut(s) 547
Bst2UI CCWGG 1 cut(s) 478
Bst4CI ACNGT 2 cut(s) 446, 467
Bst6I CTCTTC 2 cut(s) 125, 136
BstC8I GCNNGC 3 cut(s) 217, 370, 374
BstF5I GGATG 4 cut(s) 125, 134, 412, 586
BstKTI GATC 4 cut(s) 10, 166, 235, 310
BstMBI GATC 4 cut(s) 7, 163, 232, 307
BstMWI GCNNNNNNNGC 1 cut(s) 489
BstNI CCWGG 1 cut(s) 478
BstNSI RCATGY 1 cut(s) 510
BstSCI CCNGG 1 cut(s) 476
BstV1I GCAGC 3 cut(s) 39, 374, 479
BsuRI GGCC 1 cut(s) 376
BtsCI GGATG 4 cut(s) 125, 134, 412, 586
Cac8I GCNNGC 3 cut(s) 217, 370, 374
CaiI CAGNNNCTG 1 cut(s) 535
CciI TCATGA 1 cut(s) 229
Cfr10I RCCGGY 1 cut(s) 372
Cfr13I GGNCC 1 cut(s) 526
Csp6I GTAC 1 cut(s) 204
CviAII CATG 3 cut(s) 230, 507, 604
CviQI GTAC 1 cut(s) 204
DpnI GATC 4 cut(s) 9, 165, 234, 309
DpnII GATC 4 cut(s) 7, 163, 232, 307
Eam1104I CTCTTC 2 cut(s) 125, 136
EarI CTCTTC 2 cut(s) 125, 136
Eco130I CCWWGG 1 cut(s) 377
Eco47I GGWCC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 647
EcoRII CCWGG 1 cut(s) 476
EcoT14I CCWWGG 1 cut(s) 377
ErhI CCWWGG 1 cut(s) 377
FaeI CATG 3 cut(s) 233, 510, 607
FaiI YATR 8 cut(s) 24, 231, 257, 263, 472, 508, 570, 605
FaqI GGGAC 1 cut(s) 87
FatI CATG 3 cut(s) 229, 506, 603
FauI CCCGC 1 cut(s) 212
FbaI TGATCA 2 cut(s) 232, 307
Fnu4HI GCNGC 5 cut(s) 53, 213, 363, 366, 493
FokI GGATG 4 cut(s) 112, 121, 419, 573
Fsp4HI GCNGC 5 cut(s) 53, 213, 363, 366, 493
FspBI CTAG 3 cut(s) 170, 378, 648
GluI GCNGC 5 cut(s) 53, 213, 363, 366, 493
GsuI CTGGAG 2 cut(s) 321, 454
HaeIII GGCC 1 cut(s) 376
HapII CCGG 1 cut(s) 373
Hin1II CATG 3 cut(s) 233, 510, 607
HinfI GANTC 2 cut(s) 394, 576
HpaII CCGG 1 cut(s) 373
Hpy188I TCNGA 4 cut(s) 237, 393, 399, 450
Hpy188III TCNNGA 8 cut(s) 161, 170, 230, 311, 416, 460, 529, 559
HpyAV CCTTC 4 cut(s) 117, 200, 331, 566
HpyCH4III ACNGT 2 cut(s) 446, 467
HpyCH4IV ACGT 1 cut(s) 566
HpyCH4V TGCA 3 cut(s) 495, 510, 637
HpyF10VI GCNNNNNNNGC 1 cut(s) 489
HpySE526I ACGT 1 cut(s) 566
Hsp92II CATG 3 cut(s) 233, 510, 607
KroI GCCGGC 1 cut(s) 372
KroNI GCCGGC 1 cut(s) 374
Ksp22I TGATCA 2 cut(s) 232, 307
Kzo9I GATC 4 cut(s) 7, 163, 232, 307
LmnI GCTCC 2 cut(s) 302, 480
Lsp1109I GCAGC 3 cut(s) 39, 374, 479
MaeI CTAG 3 cut(s) 170, 378, 648
MaeII ACGT 1 cut(s) 566
MaeIII GTNAC 2 cut(s) 75, 559
MalI GATC 4 cut(s) 9, 165, 234, 309
MboI GATC 4 cut(s) 7, 163, 232, 307
MboII GAAGA 5 cut(s) 17, 112, 123, 158, 326
MluCI AATT 1 cut(s) 638
MlyI GAGTC 1 cut(s) 388
MroNI GCCGGC 1 cut(s) 372
MseI TTAA 5 cut(s) 38, 225, 240, 249, 330
MslI CAYNNNNRTG 1 cut(s) 557
MspA1I CMGCKG 3 cut(s) 365, 432, 492
MspI CCGG 1 cut(s) 373
MspR9I CCNGG 1 cut(s) 478
MvaI CCWGG 1 cut(s) 478
MwoI GCNNNNNNNGC 1 cut(s) 489
NaeI GCCGGC 1 cut(s) 374
NdeII GATC 4 cut(s) 7, 163, 232, 307
NgoMIV GCCGGC 1 cut(s) 372
NlaIII CATG 3 cut(s) 233, 510, 607
NmuCI GTSAC 1 cut(s) 559
NspI RCATGY 1 cut(s) 510
PagI TCATGA 1 cut(s) 229
PdiI GCCGGC 1 cut(s) 374
PfeI GAWTC 1 cut(s) 576
PkrI GCNGC 5 cut(s) 54, 214, 364, 367, 494
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PshBI ATTAAT 1 cut(s) 240
Psp6I CCWGG 1 cut(s) 476
PspGI CCWGG 1 cut(s) 476
PspPI GGNCC 1 cut(s) 526
PstNI CAGNNNCTG 1 cut(s) 535
RsaI GTAC 1 cut(s) 205
RsaNI GTAC 1 cut(s) 204
RseI CAYNNNNRTG 1 cut(s) 557
SaqAI TTAA 5 cut(s) 38, 225, 240, 249, 330
SatI GCNGC 5 cut(s) 53, 213, 363, 366, 493
Sau3AI GATC 4 cut(s) 7, 163, 232, 307
Sau96I GGNCC 1 cut(s) 526
SchI GAGTC 1 cut(s) 388
ScrFI CCNGG 1 cut(s) 478
SetI ASST 9 cut(s) 34, 44, 307, 324, 383, 485, 528, 569, 653
SinI GGWCC 1 cut(s) 526
SmiMI CAYNNNNRTG 1 cut(s) 557
SmlI CTYRAG 1 cut(s) 207
SmoI CTYRAG 1 cut(s) 207
Sse9I AATT 1 cut(s) 638
SsiI CCGC 6 cut(s) 80, 212, 219, 365, 430, 490
SspI AATATT 1 cut(s) 277
SspMI CTAG 3 cut(s) 170, 378, 648
StyD4I CCNGG 1 cut(s) 476
StyI CCWWGG 1 cut(s) 377
TaaI ACNGT 2 cut(s) 446, 467
TaiI ACGT 1 cut(s) 569
TasI AATT 1 cut(s) 638
TauI GCSGC 2 cut(s) 215, 368
TfiI GAWTC 1 cut(s) 576
Tru1I TTAA 5 cut(s) 38, 225, 240, 249, 330
Tru9I TTAA 5 cut(s) 38, 225, 240, 249, 330
TseFI GTSAC 1 cut(s) 559
TseI GCWGC 3 cut(s) 52, 362, 492
Tsp45I GTSAC 1 cut(s) 559
TspDTI ATGAA 3 cut(s) 123, 459, 585
VpaK11BI GGWCC 1 cut(s) 526
VspI ATTAAT 1 cut(s) 240
XbaI TCTAGA 1 cut(s) 169
XceI RCATGY 1 cut(s) 510
XmaJI CCTAGG 1 cut(s) 377
XspI CTAG 3 cut(s) 170, 378, 648
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.