AT1G65650

ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0014294
Physical Location & Seq
Reverse (-)
24415015 .. 24417769
2755 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G65650.1

Sequence Viewer

Length: 993 bp
ATGTCTTGGTGCACGATTGAGTCGGATCCTGGTGTATTTACAGAGCTTATTCAACAAATGCAAGTCAAAGGAGTGCAGGTTGAAGAATTGTATTCCCTGGATTCTGATTCTCTCAATAACCTCAGACCCGTATACGGTCTGATCTTTCTTTTCAAATGGCAAGCTGGGGAAAAAGATGAGCGTCCAACGATCCAAGATCAAGTTTCGAACTTATTTTTCGCAAATCAGGTCATTAACAATGCTTGTGCAACCCAAGCGATCCTTGCTATCCTCTTGAACTCTCCAGAGGTTGACATCGGGCCTGAACTATCAGCGCTGAAAGAATTCACCAAGAACTTTCCATCCGACCTCAAGGGTTTGGCTATCAATAACAGTGATTCAATCCGGGCTGCGCACAACAGTTTCGCAAGGCCTGAGCCATTTGTCCCAGAGGAACAGAAAGCTGCTACAAAAGACGATGACGTATACCATTTCATAAGCTACATACCTGTGGATGGAGTCTTGTACGAGCTTGATGGGCTCAAGGAGGGACCTATCAGTCTTGGCCCATGCCCCGGAGACCAAACTGGTATCGAGTGGCTGCAAATGGTTCAACCAGTGATCCAAGAACGGATTGAGAGGTACTCACAGAGCGAGATCAGGTTCAATCTTTTGGCTGTCATTAAAAACAGGAAGGATATCTACACTGCGGAACTCAAGGAGCTTCAAAGGCAGAGGGAACAGCTGTTGCAGCAGGCTAATACTTGTGTGGACAAAAGCGAAGCAGAAGCAGTTAATGCGTTGATTGCTGAGGTAGGCAGTGGGATCGAGGCTGCGAGTGATAAGATTGTAATGGAGGAAGAGAAGTTCATGAAATGGAGAACAGAGAACATTAGGAGGAAGCATAACTACATTCCGTTTCTGTTCAACTTCCTCAAACTTCTTGCTGAGAAGAAACAGTTGAAACCTCTGATTGAGAAGGCCAAGAAACAGAAAACAGAAAGTTCCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003824 GO:0004843 GO:0004857 GO:0004866 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009892 GO:0009894 GO:0009987 GO:0010016 GO:0010466 GO:0010605 GO:0010951 GO:0016579 GO:0016787 GO:0019222 GO:0019538 GO:0019783 GO:0030162 GO:0030234 GO:0030414 GO:0031011 GO:0031323 GO:0031324 GO:0031329 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033202 GO:0036211 GO:0036459 GO:0042176 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044877 GO:0045861 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0061136 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070603 GO:0070628 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0097346 GO:0098772 GO:0101005 GO:0140096 GO:1901564 GO:1902494 GO:1903050 GO:1903362 GO:1904949
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C12

Protein Analysis

330

Amino Acids

37.5

Weight (kDa)

5.03

Isoelectric Point (pI)

47.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_C12 PF01088 3 - 206 8.5e-66 Ubiquitin carboxyl-terminal hydrolase, family 1
UCH_C PF18031 269 - 314 5.9e-19 Ubiquitin carboxyl-terminal hydrolases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65650 AT5G16310
fragaria_vesca FvH4_4g33330 FvH4_4g33330
malus_domestica MD13G1018500.v1.1 MD16G1016200.v1.1
prunus_persica Prupe.1G335400_v2.0.a1 Prupe.1G335400_v2.0.a1
pyrus_communis pycom13g01570 pycom16g01510
rosa_chinensis RchiOBHm_Chr4g0442561
rosa_laevigata RLG00000006002
rosa_multiflora Rmu_ssc0000204.1_g000045
rosa_roxburghii Rroxscaffold_5G00383370
rosa_rugosa Rorug04G0341200
rosa_samantha Rh4AG393600
rosa_wichuraiana Rw4G034010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 537
Acc16I TGCGCA 1 cut(s) 393
Acc36I ACCTGC 1 cut(s) 67
AccI GTMKAC 2 cut(s) 132, 465
AciI CCGC 1 cut(s) 689
AclWI GGATC 6 cut(s) 20, 33, 184, 253, 595, 812
AcsI RAATTY 1 cut(s) 323
AfaI GTAC 2 cut(s) 506, 623
AfeI AGCGCT 1 cut(s) 315
AfiI CCNNNNNNNGG 3 cut(s) 134, 494, 554
AjnI CCWGG 2 cut(s) 28, 96
AloI GAACNNNNNNTCC 4 cut(s) 326, 358, 830, 862
AluBI AGCT 7 cut(s) 46, 164, 443, 480, 511, 703, 724
AluI AGCT 7 cut(s) 46, 164, 443, 480, 511, 703, 724
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 552
Alw44I GTGCAC 1 cut(s) 10
AlwI GGATC 6 cut(s) 20, 33, 184, 253, 595, 812
Aor51HI AGCGCT 1 cut(s) 315
AoxI GGCC 4 cut(s) 299, 410, 544, 960
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 5 cut(s) 389, 443, 580, 730, 812
ApoI RAATTY 1 cut(s) 323
Asp700I GAANNNNTTC 1 cut(s) 323
AspLEI GCGC 2 cut(s) 316, 394
AspS9I GGNCC 3 cut(s) 299, 530, 545
AsuC2I CCSGG 2 cut(s) 386, 555
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 206
AvaII GGWCC 1 cut(s) 530
BaeGI GKGCMC 1 cut(s) 14
BamHI GGATCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 522
BarI GAAGNNNNNNTAC 4 cut(s) 665, 697, 872, 904
Bbv12I GWGCWC 1 cut(s) 14
BbvCI CCTCAGC 1 cut(s) 789
BbvI GCAGC 5 cut(s) 376, 430, 567, 742, 799
BccI CCATC 3 cut(s) 349, 488, 509
BcgI CGANNNNNNTGC 2 cut(s) 787, 821
BciT130I CCWGG 2 cut(s) 30, 98
BcnI CCSGG 2 cut(s) 386, 555
BcoDI GTCTC 1 cut(s) 552
BfoI RGCGCY 1 cut(s) 317
BfuAI ACCTGC 1 cut(s) 67
BisI GCNGC 5 cut(s) 390, 444, 581, 731, 813
BlsI GCNGC 5 cut(s) 391, 445, 582, 732, 814
Bme1390I CCNGG 4 cut(s) 30, 98, 386, 555
Bme18I GGWCC 1 cut(s) 530
BmgT120I GGNCC 3 cut(s) 299, 530, 545
BmiI GGNNCC 2 cut(s) 27, 531
BmrFI CCNGG 4 cut(s) 30, 98, 386, 555
BplI GAGNNNNNCTC 2 cut(s) 608, 640
BpmI CTGGAG 1 cut(s) 267
Bpu10I CCTNAGC 2 cut(s) 414, 789
Bpu14I TTCGAA 1 cut(s) 206
BpuEI CTTGAG 3 cut(s) 335, 506, 680
BpuMI CCSGG 2 cut(s) 386, 555
BsaI GGTCTC 1 cut(s) 552
BsaJI CCNNGG 2 cut(s) 96, 553
BsaXI ACNNNNNCTCC 2 cut(s) 63, 93
Bsc4I CCNNNNNNNGG 3 cut(s) 134, 494, 554
Bse1I ACTGG 2 cut(s) 571, 596
BseBI CCWGG 2 cut(s) 30, 98
BseDI CCNNGG 2 cut(s) 96, 553
BseGI GGATG 2 cut(s) 341, 499
BseLI CCNNNNNNNGG 3 cut(s) 134, 494, 554
BseMII CTCAG 4 cut(s) 136, 405, 780, 918
BseNI ACTGG 2 cut(s) 571, 596
BseSI GKGCMC 1 cut(s) 14
BseXI GCAGC 5 cut(s) 376, 430, 567, 742, 799
BseYI CCCAGC 1 cut(s) 164
BsgI GTGCAG 1 cut(s) 95
BshFI GGCC 4 cut(s) 301, 412, 546, 962
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 2 cut(s) 385, 555
BslFI GGGAC 2 cut(s) 410, 543
BslI CCNNNNNNNGG 3 cut(s) 134, 494, 554
BsmAI GTCTC 1 cut(s) 552
BsmFI GGGAC 2 cut(s) 410, 543
BsnI GGCC 4 cut(s) 301, 412, 546, 962
Bso31I GGTCTC 1 cut(s) 552
Bsp119I TTCGAA 1 cut(s) 206
Bsp1286I GDGCHC 2 cut(s) 14, 522
Bsp143I GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
BspACI CCGC 1 cut(s) 689
BspANI GGCC 4 cut(s) 301, 412, 546, 962
BspCNI CTCAG 4 cut(s) 135, 406, 781, 919
BspHI TCATGA 1 cut(s) 849
BspLI GGNNCC 2 cut(s) 27, 531
BspMI ACCTGC 1 cut(s) 67
BspPI GGATC 6 cut(s) 20, 33, 184, 253, 595, 812
BspT104I TTCGAA 1 cut(s) 206
BspTNI GGTCTC 1 cut(s) 552
BsrI ACTGG 2 cut(s) 571, 596
BssECI CCNNGG 2 cut(s) 96, 553
BssMI GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
BssNAI GTATAC 2 cut(s) 133, 466
Bst1107I GTATAC 2 cut(s) 133, 466
Bst2UI CCWGG 2 cut(s) 30, 98
Bst4CI ACNGT 4 cut(s) 137, 374, 401, 939
Bst6I CTCTTC 1 cut(s) 834
BstAPI GCANNNNNTGC 1 cut(s) 776
BstBI TTCGAA 1 cut(s) 206
BstC8I GCNNGC 2 cut(s) 162, 735
BstDEI CTNAG 4 cut(s) 122, 414, 789, 927
BstF5I GGATG 2 cut(s) 341, 499
BstH2I RGCGCY 1 cut(s) 317
BstHHI GCGC 2 cut(s) 316, 394
BstKTI GATC 8 cut(s) 28, 144, 192, 199, 261, 603, 639, 807
BstMAI GTCTC 1 cut(s) 552
BstMBI GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
BstMWI GCNNNNNNNGC 7 cut(s) 254, 263, 517, 709, 730, 776, 785
BstNI CCWGG 2 cut(s) 30, 98
BstSCI CCNGG 4 cut(s) 28, 96, 384, 553
BstSLI GKGCMC 1 cut(s) 14
BstV1I GCAGC 5 cut(s) 376, 430, 567, 742, 799
BstX2I RGATCY 1 cut(s) 25
BstYI RGATCY 1 cut(s) 25
BstZ17I GTATAC 2 cut(s) 133, 466
BsuRI GGCC 4 cut(s) 301, 412, 546, 962
BtsCI GGATG 2 cut(s) 341, 499
BtsI GCAGTG 2 cut(s) 684, 805
BtsIMutI CAGTG 4 cut(s) 379, 603, 684, 805
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 2 cut(s) 162, 735
CciI TCATGA 1 cut(s) 849
CfoI GCGC 2 cut(s) 316, 394
Cfr13I GGNCC 3 cut(s) 299, 530, 545
CseI GACGC 1 cut(s) 170
Csp6I GTAC 2 cut(s) 505, 622
CviAII CATG 2 cut(s) 549, 850
CviQI GTAC 2 cut(s) 505, 622
DdeI CTNAG 4 cut(s) 122, 414, 789, 927
DpnI GATC 8 cut(s) 27, 143, 191, 198, 260, 602, 638, 806
DpnII GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
DrdI GACNNNNNNGTC 1 cut(s) 537
DseDI GACNNNNNNGTC 1 cut(s) 537
Eam1104I CTCTTC 1 cut(s) 834
EarI CTCTTC 1 cut(s) 834
Eco147I AGGCCT 1 cut(s) 412
Eco24I GRGCYC 1 cut(s) 522
Eco31I GGTCTC 1 cut(s) 552
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 1 cut(s) 530
Eco47III AGCGCT 1 cut(s) 315
EcoO109I RGGNCCY 1 cut(s) 530
EcoRI GAATTC 1 cut(s) 323
EcoRII CCWGG 2 cut(s) 28, 96
EcoRV GATATC 1 cut(s) 679
EcoT38I GRGCYC 1 cut(s) 522
FaeI CATG 2 cut(s) 552, 853
FaiI YATR 7 cut(s) 133, 466, 476, 485, 550, 851, 885
FalI AAGNNNNNCTT 3 cut(s) 246, 278, 973
FaqI GGGAC 2 cut(s) 410, 543
FatI CATG 2 cut(s) 548, 849
FblI GTMKAC 2 cut(s) 132, 465
Fnu4HI GCNGC 5 cut(s) 390, 444, 581, 731, 813
FokI GGATG 2 cut(s) 328, 506
FriOI GRGCYC 1 cut(s) 522
Fsp4HI GCNGC 5 cut(s) 390, 444, 581, 731, 813
FspI TGCGCA 1 cut(s) 393
GlaI GCGC 2 cut(s) 315, 393
GluI GCNGC 5 cut(s) 390, 444, 581, 731, 813
GsaI CCCAGC 1 cut(s) 168
GsuI CTGGAG 1 cut(s) 267
HaeII RGCGCY 1 cut(s) 317
HaeIII GGCC 4 cut(s) 301, 412, 546, 962
HapII CCGG 2 cut(s) 385, 555
HgaI GACGC 1 cut(s) 170
HhaI GCGC 2 cut(s) 316, 394
Hin1II CATG 2 cut(s) 552, 853
Hin6I GCGC 2 cut(s) 314, 392
HinP1I GCGC 2 cut(s) 314, 392
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 5 cut(s) 20, 101, 107, 377, 498
HpaII CCGG 2 cut(s) 385, 555
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 5 cut(s) 12, 133, 292, 466, 751
Hpy188I TCNGA 6 cut(s) 25, 106, 125, 141, 346, 951
Hpy188III TCNNGA 3 cut(s) 274, 284, 850
Hpy8I GTNNAC 5 cut(s) 12, 133, 292, 466, 751
HpyAV CCTTC 2 cut(s) 667, 952
HpyCH4III ACNGT 4 cut(s) 137, 374, 401, 939
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 6 cut(s) 12, 61, 76, 248, 583, 730
HpyF10VI GCNNNNNNNGC 7 cut(s) 254, 263, 517, 709, 730, 776, 785
HpyF3I CTNAG 4 cut(s) 122, 414, 789, 927
HpySE526I ACGT 1 cut(s) 462
Hsp92II CATG 2 cut(s) 552, 853
HspAI GCGC 2 cut(s) 314, 392
Kzo9I GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
LmnI GCTCC 1 cut(s) 700
Lsp1109I GCAGC 5 cut(s) 376, 430, 567, 742, 799
MaeII ACGT 1 cut(s) 462
MalI GATC 8 cut(s) 27, 143, 191, 198, 260, 602, 638, 806
MboI GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
MboII GAAGA 3 cut(s) 95, 851, 943
MflI RGATCY 1 cut(s) 25
MhlI GDGCHC 2 cut(s) 14, 522
MluCI AATT 2 cut(s) 86, 323
MlyI GAGTC 2 cut(s) 29, 507
MmeI TCCRAC 2 cut(s) 209, 369
MroXI GAANNNNTTC 1 cut(s) 323
MseI TTAA 3 cut(s) 234, 663, 774
MslI CAYNNNNRTG 1 cut(s) 488
MspA1I CMGCKG 1 cut(s) 724
MspI CCGG 2 cut(s) 385, 555
MspR9I CCNGG 4 cut(s) 30, 98, 386, 555
MvaI CCWGG 2 cut(s) 30, 98
MwoI GCNNNNNNNGC 7 cut(s) 254, 263, 517, 709, 730, 776, 785
NciI CCSGG 2 cut(s) 386, 555
NdeII GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
NlaIII CATG 2 cut(s) 552, 853
NlaIV GGNNCC 2 cut(s) 27, 531
NsbI TGCGCA 1 cut(s) 393
NspV TTCGAA 1 cut(s) 206
PagI TCATGA 1 cut(s) 849
PceI AGGCCT 1 cut(s) 412
PdmI GAANNNNTTC 1 cut(s) 323
PfeI GAWTC 3 cut(s) 101, 107, 377
PkrI GCNGC 5 cut(s) 391, 445, 582, 732, 814
PleI GAGTC 2 cut(s) 28, 506
PpsI GAGTC 2 cut(s) 28, 506
PpuMI RGGWCCY 1 cut(s) 530
Psp5II RGGWCCY 1 cut(s) 530
Psp6I CCWGG 2 cut(s) 28, 96
PspFI CCCAGC 1 cut(s) 164
PspGI CCWGG 2 cut(s) 28, 96
PspN4I GGNNCC 2 cut(s) 27, 531
PspPI GGNCC 3 cut(s) 299, 530, 545
PspPPI RGGWCCY 1 cut(s) 530
PsuI RGATCY 1 cut(s) 25
PvuII CAGCTG 1 cut(s) 724
RsaI GTAC 2 cut(s) 506, 623
RsaNI GTAC 2 cut(s) 505, 622
RseI CAYNNNNRTG 1 cut(s) 488
SaqAI TTAA 3 cut(s) 234, 663, 774
SatI GCNGC 5 cut(s) 390, 444, 581, 731, 813
Sau3AI GATC 8 cut(s) 25, 141, 189, 196, 258, 600, 636, 804
Sau96I GGNCC 3 cut(s) 299, 530, 545
SchI GAGTC 2 cut(s) 29, 507
ScrFI CCNGG 4 cut(s) 30, 98, 386, 555
SduI GDGCHC 2 cut(s) 14, 522
SfuI TTCGAA 1 cut(s) 206
SinI GGWCC 1 cut(s) 530
SmiMI CAYNNNNRTG 1 cut(s) 488
SmlI CTYRAG 3 cut(s) 350, 521, 695
SmoI CTYRAG 3 cut(s) 350, 521, 695
Sse9I AATT 2 cut(s) 86, 323
SseBI AGGCCT 1 cut(s) 412
SsiI CCGC 1 cut(s) 689
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 4 cut(s) 28, 96, 384, 553
TaaI ACNGT 4 cut(s) 137, 374, 401, 939
TaiI ACGT 1 cut(s) 465
TaqI TCGA 3 cut(s) 206, 573, 807
TasI AATT 2 cut(s) 86, 323
TfiI GAWTC 3 cut(s) 101, 107, 377
Tru1I TTAA 3 cut(s) 234, 663, 774
Tru9I TTAA 3 cut(s) 234, 663, 774
TscAI CASTG 4 cut(s) 379, 603, 691, 805
TseI GCWGC 5 cut(s) 389, 443, 580, 730, 812
TspDTI ATGAA 3 cut(s) 463, 838, 866
TspGWI ACGGA 2 cut(s) 625, 885
TspRI CASTG 4 cut(s) 379, 603, 691, 805
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 1 cut(s) 530
XapI RAATTY 1 cut(s) 323
XmiI GTMKAC 2 cut(s) 132, 465
XmnI GAANNNNTTC 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.