Rorug04G0341200

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0000875
Physical Location & Seq
Forward (+)
51390805 .. 51393617
2813 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0341200.1

Sequence Viewer

Length: 966 bp
ATGAGCATGAAAGTTGAAAGCTTTCCCAGTAAGAGAGCCATTTCAGCATTCAAACGGAGCTTTCATTCCGGCCACGTTTTGAAACAGTGTGTTTCCAATGTGAAACTGAGTAAAGAAGAGTTTGTGTCCAAATTGCTTGATAGAAGATGGACATTGCCTATCCCTGATGCTAAAATTCACCAAATAAAGCTTTCAACCAGCAAAGTACAACCACAACCAGGCAGACCCTTGTCTGATATTTCCTTCTGGAACAATACTAACCCATGTTTTAGTCATTCTATGGCGGAAAAGGACGATTCTTTCTTTATTGTGAGGGATGATTTGTTGCATCCGTTGATAAATGGGAATAAAGCAAGAAAATTGGATGCATTGATTCCCCTTGTTGAAGATCATTCCGTGACTGATGTGGTGACATGTGGAGGTTGTCAAAGTGCCCATACAGCAGCTGTTGCCGTTTCATGTTCAGAAAGAGGATTAAAACCACATTTACTTCTACGAGGGGAGCAGCCTGAAATACTGACAGGCTATAACTTGATGTCAACAATATATGGCAATGTCACATATGTTCCAAGATCGCTTTATGCCAATAGGGAAAAGATGTTAAAGAGCCATGCTGATTATTTGGCAGGCAGTAGTGGTAACGTCCTTCGGTTTGATGATATTTTGGAGGCTTCTTTGAATCAAAGTGATGGTGCTCTGAATCTTTTGCAAAAGGATGCTAATAGAAGTGACCATCCTCGGAAAATTGTAATTGTCAATGAAGGCGCAGGAGATGTTGTAGCATTACTAGGTTTGATTCGCCTAGTGCAGTACTTATCCCAGAATCACTTACTTGGAAAAGAGAGGGTCTTGAAGTTTGTTGTAGATTCTGGAACTGGGACAACAGCTGTTGGTTTAGCTCTTGGAGCCATATGTTTGGGGTGTGTTCCCGCTCCCATGGGAGGTAACTGCAGTGATGTTGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

321

Amino Acids

34.96

Weight (kDa)

8.72

Isoelectric Point (pI)

32.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 932
AciI CCGC 2 cut(s) 284, 930
AcoI YGGCCR 1 cut(s) 70
AcsI RAATTY 1 cut(s) 174
AfaI GTAC 2 cut(s) 207, 812
AfiI CCNNNNNNNGG 2 cut(s) 218, 941
AflIII ACRYGT 1 cut(s) 413
AgsI TTSAA 7 cut(s) 17, 52, 82, 195, 386, 679, 853
AjnI CCWGG 1 cut(s) 217
AluBI AGCT 6 cut(s) 21, 60, 190, 446, 887, 899
AluI AGCT 6 cut(s) 21, 60, 190, 446, 887, 899
Alw21I GWGCWC 1 cut(s) 697
AlwNI CAGNNNCTG 1 cut(s) 446
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 2 cut(s) 443, 505
ApoI RAATTY 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 21
AspLEI GCGC 1 cut(s) 767
AsuHPI GGTGA 2 cut(s) 170, 421
BaeGI GKGCMC 1 cut(s) 436
Bbv12I GWGCWC 1 cut(s) 697
BbvI GCAGC 2 cut(s) 455, 517
BccI CCATC 3 cut(s) 141, 683, 741
BceAI ACGGC 1 cut(s) 437
BciT130I CCWGG 1 cut(s) 219
BfaI CTAG 2 cut(s) 788, 803
BfmI CTRYAG 1 cut(s) 949
BisI GCNGC 2 cut(s) 444, 506
BlsI GCNGC 2 cut(s) 445, 507
BmcAI AGTACT 1 cut(s) 812
Bme1390I CCNGG 1 cut(s) 219
BmiI GGNNCC 1 cut(s) 907
BmrFI CCNGG 1 cut(s) 219
BmrI ACTGGG 2 cut(s) 21, 885
BmsI GCATC 4 cut(s) 157, 337, 355, 706
BmuI ACTGGG 2 cut(s) 21, 885
BoxI GACNNNNGTC 1 cut(s) 229
BsaJI CCNNGG 2 cut(s) 737, 936
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 941
Bse1I ACTGG 2 cut(s) 27, 880
Bse3DI GCAATG 2 cut(s) 152, 559
BseBI CCWGG 1 cut(s) 219
BseDI CCNNGG 2 cut(s) 737, 936
BseGI GGATG 5 cut(s) 322, 328, 370, 721, 733
BseLI CCNNNNNNNGG 2 cut(s) 218, 941
BseMI GCAATG 2 cut(s) 152, 559
BseMII CTCAG 1 cut(s) 98
BseNI ACTGG 2 cut(s) 27, 880
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 2 cut(s) 455, 517
BsgI GTGCAG 1 cut(s) 827
BshFI GGCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 697
BsiSI CCGG 1 cut(s) 69
BslFI GGGAC 1 cut(s) 892
BslI CCNNNNNNNGG 2 cut(s) 218, 941
BsmFI GGGAC 1 cut(s) 892
BsmI GAATGC 1 cut(s) 47
BsnI GGCC 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 436, 697
Bsp143I GATC 2 cut(s) 388, 572
Bsp19I CCATGG 1 cut(s) 936
BspACI CCGC 2 cut(s) 284, 930
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 907
BspMAI CTGCAG 1 cut(s) 953
BsrBI CCGCTC 1 cut(s) 932
BsrDI GCAATG 2 cut(s) 152, 559
BsrI ACTGG 2 cut(s) 27, 880
BssECI CCNNGG 2 cut(s) 737, 936
BssMI GATC 2 cut(s) 388, 572
BssT1I CCWWGG 1 cut(s) 936
Bst2UI CCWGG 1 cut(s) 219
Bst4CI ACNGT 1 cut(s) 87
Bst6I CTCTTC 1 cut(s) 111
BstAPI GCANNNNNTGC 1 cut(s) 449
BstC8I GCNNGC 1 cut(s) 628
BstDEI CTNAG 1 cut(s) 107
BstDSI CCRYGG 1 cut(s) 936
BstF5I GGATG 5 cut(s) 322, 328, 370, 721, 733
BstHHI GCGC 1 cut(s) 767
BstKTI GATC 2 cut(s) 391, 575
BstMBI GATC 2 cut(s) 388, 572
BstMWI GCNNNNNNNGC 4 cut(s) 44, 440, 449, 905
BstNI CCWGG 1 cut(s) 219
BstNSI RCATGY 1 cut(s) 417
BstPAI GACNNNNGTC 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 217
BstSFI CTRYAG 1 cut(s) 949
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 2 cut(s) 455, 517
BstXI CCANNNNNNTGG 1 cut(s) 916
BsuRI GGCC 1 cut(s) 72
BtgI CCRYGG 1 cut(s) 936
BtsCI GGATG 5 cut(s) 322, 328, 370, 721, 733
BtsI GCAGTG 1 cut(s) 958
BtsIMutI CAGTG 2 cut(s) 92, 958
Cac8I GCNNGC 1 cut(s) 628
CaiI CAGNNNCTG 1 cut(s) 446
CfoI GCGC 1 cut(s) 767
Csp6I GTAC 2 cut(s) 206, 811
CviAII CATG 6 cut(s) 7, 264, 414, 459, 611, 937
CviQI GTAC 2 cut(s) 206, 811
DdeI CTNAG 1 cut(s) 107
DpnI GATC 2 cut(s) 390, 574
DpnII GATC 2 cut(s) 388, 572
EaeI YGGCCR 1 cut(s) 70
Eam1104I CTCTTC 1 cut(s) 111
EarI CTCTTC 1 cut(s) 111
EciI GGCGGA 1 cut(s) 299
Eco130I CCWWGG 1 cut(s) 936
EcoRII CCWGG 1 cut(s) 217
EcoT14I CCWWGG 1 cut(s) 936
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 6 cut(s) 10, 267, 417, 462, 614, 940
FaqI GGGAC 1 cut(s) 892
FatI CATG 6 cut(s) 6, 263, 413, 458, 610, 936
FauI CCCGC 1 cut(s) 937
FauNDI CATATG 2 cut(s) 562, 911
Fnu4HI GCNGC 2 cut(s) 444, 506
FokI GGATG 5 cut(s) 315, 329, 377, 720, 728
Fsp4HI GCNGC 2 cut(s) 444, 506
FspBI CTAG 2 cut(s) 788, 803
GlaI GCGC 1 cut(s) 766
GluI GCNGC 2 cut(s) 444, 506
HaeIII GGCC 1 cut(s) 72
HapII CCGG 1 cut(s) 69
HhaI GCGC 1 cut(s) 767
Hin1II CATG 6 cut(s) 10, 267, 417, 462, 614, 940
Hin6I GCGC 1 cut(s) 765
HinP1I GCGC 1 cut(s) 765
HincII GTYRAC 1 cut(s) 540
HindII GTYRAC 1 cut(s) 540
HindIII AAGCTT 2 cut(s) 19, 188
HinfI GANTC 7 cut(s) 296, 373, 679, 700, 796, 823, 866
HpaII CCGG 1 cut(s) 69
HphI GGTGA 2 cut(s) 170, 421
Hpy166II GTNNAC 1 cut(s) 540
Hpy188I TCNGA 4 cut(s) 235, 466, 699, 741
Hpy188III TCNNGA 3 cut(s) 247, 850, 870
Hpy8I GTNNAC 1 cut(s) 540
HpyAV CCTTC 3 cut(s) 253, 656, 755
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4IV ACGT 2 cut(s) 75, 642
HpyCH4V TGCA 5 cut(s) 328, 368, 709, 808, 951
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 440, 449, 905
HpyF3I CTNAG 1 cut(s) 107
HpySE526I ACGT 2 cut(s) 75, 642
Hsp92II CATG 6 cut(s) 10, 267, 417, 462, 614, 940
HspAI GCGC 1 cut(s) 765
Kzo9I GATC 2 cut(s) 388, 572
LmnI GCTCC 4 cut(s) 57, 502, 905, 937
Lsp1109I GCAGC 2 cut(s) 455, 517
LweI GCATC 4 cut(s) 157, 337, 355, 706
MaeI CTAG 2 cut(s) 788, 803
MaeII ACGT 2 cut(s) 75, 642
MaeIII GTNAC 6 cut(s) 397, 409, 556, 638, 728, 944
MalI GATC 2 cut(s) 390, 574
MbiI CCGCTC 1 cut(s) 932
MboI GATC 2 cut(s) 388, 572
MboII GAAGA 3 cut(s) 128, 156, 398
MhlI GDGCHC 2 cut(s) 436, 697
MluCI AATT 5 cut(s) 131, 174, 359, 744, 750
MnlI CCTC 8 cut(s) 306, 413, 464, 491, 661, 747, 837, 935
Mph1103I ATGCAT 1 cut(s) 370
MroXI GAANNNNTTC 1 cut(s) 21
MseI TTAA 2 cut(s) 476, 602
MspA1I CMGCKG 2 cut(s) 446, 887
MspI CCGG 1 cut(s) 69
MspR9I CCNGG 1 cut(s) 219
Mva1269I GAATGC 1 cut(s) 47
MvaI CCWGG 1 cut(s) 219
MwoI GCNNNNNNNGC 4 cut(s) 44, 440, 449, 905
NcoI CCATGG 1 cut(s) 936
NdeI CATATG 2 cut(s) 562, 911
NdeII GATC 2 cut(s) 388, 572
NlaIII CATG 6 cut(s) 10, 267, 417, 462, 614, 940
NlaIV GGNNCC 1 cut(s) 907
NmuCI GTSAC 4 cut(s) 397, 409, 556, 728
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 417
PciI ACATGT 1 cut(s) 413
PctI GAATGC 1 cut(s) 47
PdmI GAANNNNTTC 1 cut(s) 21
PfeI GAWTC 7 cut(s) 296, 373, 679, 700, 796, 823, 866
PkrI GCNGC 2 cut(s) 445, 507
PscI ACATGT 1 cut(s) 413
PshAI GACNNNNGTC 1 cut(s) 229
Psp6I CCWGG 1 cut(s) 217
PspGI CCWGG 1 cut(s) 217
PspN4I GGNNCC 1 cut(s) 907
PstI CTGCAG 1 cut(s) 953
PstNI CAGNNNCTG 1 cut(s) 446
PvuII CAGCTG 2 cut(s) 446, 887
RsaI GTAC 2 cut(s) 207, 812
RsaNI GTAC 2 cut(s) 206, 811
SaqAI TTAA 2 cut(s) 476, 602
SatI GCNGC 2 cut(s) 444, 506
Sau3AI GATC 2 cut(s) 388, 572
ScaI AGTACT 1 cut(s) 812
ScrFI CCNGG 1 cut(s) 219
SduI GDGCHC 2 cut(s) 436, 697
SfaNI GCATC 4 cut(s) 157, 337, 355, 706
SfcI CTRYAG 1 cut(s) 949
Sse9I AATT 5 cut(s) 131, 174, 359, 744, 750
SsiI CCGC 2 cut(s) 284, 930
SspMI CTAG 2 cut(s) 788, 803
StyD4I CCNGG 1 cut(s) 217
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 1 cut(s) 87
TaiI ACGT 2 cut(s) 78, 645
TasI AATT 5 cut(s) 131, 174, 359, 744, 750
TatI WGTACW 2 cut(s) 205, 810
TfiI GAWTC 7 cut(s) 296, 373, 679, 700, 796, 823, 866
Tru1I TTAA 2 cut(s) 476, 602
Tru9I TTAA 2 cut(s) 476, 602
TscAI CASTG 2 cut(s) 92, 958
TseFI GTSAC 4 cut(s) 397, 409, 556, 728
TseI GCWGC 2 cut(s) 443, 505
Tsp45I GTSAC 4 cut(s) 397, 409, 556, 728
TspDTI ATGAA 4 cut(s) 23, 53, 447, 774
TspGWI ACGGA 3 cut(s) 70, 321, 385
TspRI CASTG 2 cut(s) 92, 958
XapI RAATTY 1 cut(s) 174
XceI RCATGY 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 21
XspI CTAG 2 cut(s) 788, 803
ZrmI AGTACT 1 cut(s) 812
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.