Rorug03G0111000

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000875
Physical Location & Seq
Forward (+)
8977920 .. 8978220
301 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0111000.1

Sequence Viewer

Length: 216 bp
ATGTGGGTTCTCTTTGAATTGCTTGAATACCACCTGCTCACTTTGGTTGTGCACCTCTTGATTGTTGTTCTTCTTGTGGTCCAATGCTTTAAATTCATTAACAAGTCTCCACCCAAGATTCCAGATATTCAAATTCCAGAGAAGCTATTTCTACAGATTGTCTCTGCAATTACATTTGAGATCTACTGGGATTTTGTTGTCATTAGGGATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

71

Amino Acids

8.45

Weight (kDa)

5.45

Isoelectric Point (pI)

21.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Reticulon PF02453 1 - 71 5.3e-10 Reticulon
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 42
Acc36I ACCTGC 1 cut(s) 42
AcsI RAATTY 2 cut(s) 92, 132
AgsI TTSAA 3 cut(s) 17, 26, 131
AluBI AGCT 1 cut(s) 145
AluI AGCT 1 cut(s) 145
Alw21I GWGCWC 1 cut(s) 54
Alw26I GTCTC 2 cut(s) 111, 166
Alw44I GTGCAC 1 cut(s) 50
ApaLI GTGCAC 1 cut(s) 50
ApoI RAATTY 2 cut(s) 92, 132
AspS9I GGNCC 1 cut(s) 79
AvaII GGWCC 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 54
Bbv12I GWGCWC 1 cut(s) 54
BcoDI GTCTC 2 cut(s) 111, 166
BfmI CTRYAG 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 42
BglII AGATCT 1 cut(s) 180
Bme18I GGWCC 1 cut(s) 79
BmgT120I GGNCC 1 cut(s) 79
BmrI ACTGGG 1 cut(s) 196
BmuI ACTGGG 1 cut(s) 196
Bse1I ACTGG 1 cut(s) 191
BseNI ACTGG 1 cut(s) 191
BseSI GKGCMC 1 cut(s) 54
BsiHKAI GWGCWC 1 cut(s) 54
BsmAI GTCTC 2 cut(s) 111, 166
Bsp1286I GDGCHC 1 cut(s) 54
Bsp143I GATC 1 cut(s) 180
BspMI ACCTGC 1 cut(s) 42
BsrI ACTGG 1 cut(s) 191
BssMI GATC 1 cut(s) 180
BstKTI GATC 1 cut(s) 183
BstMAI GTCTC 2 cut(s) 111, 166
BstMBI GATC 1 cut(s) 180
BstSFI CTRYAG 1 cut(s) 152
BstSLI GKGCMC 1 cut(s) 54
BstX2I RGATCY 1 cut(s) 180
BstYI RGATCY 1 cut(s) 180
BveI ACCTGC 1 cut(s) 42
Cfr13I GGNCC 1 cut(s) 79
CviJI RGCY 1 cut(s) 145
CviKI_1 RGCY 1 cut(s) 145
DpnI GATC 1 cut(s) 182
DpnII GATC 1 cut(s) 180
DraI TTTAAA 1 cut(s) 91
Eco47I GGWCC 1 cut(s) 79
FaiI YATR 2 cut(s) 212, 214
HinfI GANTC 1 cut(s) 118
Hpy166II GTNNAC 1 cut(s) 52
Hpy188III TCNNGA 3 cut(s) 58, 122, 137
Hpy8I GTNNAC 1 cut(s) 52
HpyCH4V TGCA 2 cut(s) 52, 167
Kzo9I GATC 1 cut(s) 180
LpnPI CCDG 4 cut(s) 47, 135, 150, 172
MalI GATC 1 cut(s) 182
MboI GATC 1 cut(s) 180
MboII GAAGA 1 cut(s) 62
MflI RGATCY 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 54
MluCI AATT 4 cut(s) 17, 92, 132, 168
MnlI CCTC 1 cut(s) 65
MseI TTAA 2 cut(s) 90, 99
NdeII GATC 1 cut(s) 180
PaqCI CACCTGC 1 cut(s) 42
PfeI GAWTC 1 cut(s) 118
PspPI GGNCC 1 cut(s) 79
PsuI RGATCY 1 cut(s) 180
SaqAI TTAA 2 cut(s) 90, 99
Sau3AI GATC 1 cut(s) 180
Sau96I GGNCC 1 cut(s) 79
SduI GDGCHC 1 cut(s) 54
SetI ASST 3 cut(s) 36, 57, 147
SfcI CTRYAG 1 cut(s) 152
SgeI CNNG 9 cut(s) 35, 46, 70, 86, 115, 127, 134, 149, 199
SinI GGWCC 1 cut(s) 79
Sse9I AATT 4 cut(s) 17, 92, 132, 168
TasI AATT 4 cut(s) 17, 92, 132, 168
TfiI GAWTC 1 cut(s) 118
Tru1I TTAA 2 cut(s) 90, 99
Tru9I TTAA 2 cut(s) 90, 99
TspDTI ATGAA 1 cut(s) 85
VneI GTGCAC 1 cut(s) 50
VpaK11BI GGWCC 1 cut(s) 79
XapI RAATTY 2 cut(s) 92, 132
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.