pycom14g01180

catalytic domain of ctd-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0000875
Physical Location & Seq
Reverse (-)
828209 .. 830441
2233 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g01180.1

Sequence Viewer

Length: 789 bp
ATGGCTGATCAAAAGATCTGTAAAAACAAGCATCAATATGACAGCGAAGACGGCGACGATGATGTCAACAAAGACACCGGAAGCGATTGCGGCCTTTCTCTGGAGAAGCTAAACCTAGGATCAAGGAAGAAGCTTGTAATCTTTAGCCTCAATGGCTTGATAGTCCACAGAGTTTCCCTCCACTACGGGACAAAAATTCCGAGCAATCGTACTCCCGATGGCACATATGGAAGCTATCTAGTTTTTAGGAGACCATTTTGTGAGGAATTCATGCAATTTTGTTTTGAAAGATTTGAAGTTGGAATATGGAGTTCTGCCCAAGAAAAAAATGTGGATGGTGTGTTGGATTGTATAATGGGAAAGCTGAGAAGACGACTGGCATTTGTTTGGGATCAACACCTTGAATGTACCGACTCTGGGTTGAAGTCGCTGGAGAATAAAAACAAGCCTCTATTTTTCAAAGAACTGGACAAAGTGTGGAGCTATTTTAGTGGGAAATATTCAGAATCTGATACACTTTCCATTGATGACAAACCTTACAAGGCTCTATTGAATCCTCCTAACACAGGTATCTTCTTGGAGTCATATGATGCTGAGAATGACAACGACAAAGAATTAGATCCAAAGGGAGAACTTGGGAAGTATTTGGATGGCCTTGCAGATGCAAAAGATGTGCAAATTTATGTGAAGGGAAACCCTTTTGGAATGCCTGCAATTTCACCTACTCATCCGGATTGGAAATTCTACTCGAATGTTCTTCGTAGACTCGGAAAGCGGTACAAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

263

Amino Acids

30.05

Weight (kDa)

6.54

Isoelectric Point (pI)

35.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 44 - 226 3.3e-20 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AccI GTMKAC 1 cut(s) 763
AccIII TCCGGA 1 cut(s) 730
AciI CCGC 2 cut(s) 90, 775
AclWI GGATC 3 cut(s) 127, 399, 614
AcsI RAATTY 4 cut(s) 195, 266, 678, 740
AfaI GTAC 3 cut(s) 211, 409, 779
AfiI CCNNNNNNNGG 3 cut(s) 100, 417, 566
AgsI TTSAA 6 cut(s) 287, 296, 404, 424, 460, 553
AluBI AGCT 5 cut(s) 109, 133, 234, 364, 483
AluI AGCT 5 cut(s) 109, 133, 234, 364, 483
Alw26I GTCTC 1 cut(s) 244
AlwI GGATC 3 cut(s) 127, 399, 614
AlwNI CAGNNNCTG 1 cut(s) 509
Aor13HI TCCGGA 1 cut(s) 730
AoxI GGCC 2 cut(s) 91, 652
ApoI RAATTY 4 cut(s) 195, 266, 678, 740
AspA2I CCTAGG 1 cut(s) 115
AsuHPI GGTGA 1 cut(s) 711
AvrII CCTAGG 1 cut(s) 115
BaeI ACNNNNGTAYC 3 cut(s) 553, 586, 769
BbsI GAAGAC 2 cut(s) 54, 376
BccI CCATC 3 cut(s) 212, 329, 644
BceAI ACGGC 1 cut(s) 67
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 244
BfaI CTAG 2 cut(s) 116, 239
BglI GCCNNNNNGGC 1 cut(s) 153
BglII AGATCT 1 cut(s) 15
BisI GCNGC 1 cut(s) 91
BlnI CCTAGG 1 cut(s) 115
BlsI GCNGC 1 cut(s) 92
BmsI GCATC 3 cut(s) 40, 580, 652
BpiI GAAGAC 2 cut(s) 54, 376
BplI GAGNNNNNCTC 2 cut(s) 162, 194
BpmI CTGGAG 2 cut(s) 122, 452
BsaI GGTCTC 1 cut(s) 244
BsaJI CCNNGG 1 cut(s) 115
BsaWI WCCGGW 2 cut(s) 77, 730
Bsc4I CCNNNNNNNGG 3 cut(s) 100, 417, 566
Bse1I ACTGG 2 cut(s) 381, 471
BseAI TCCGGA 1 cut(s) 730
BseDI CCNNGG 1 cut(s) 115
BseGI GGATG 3 cut(s) 340, 655, 727
BseLI CCNNNNNNNGG 3 cut(s) 100, 417, 566
BseMII CTCAG 2 cut(s) 356, 585
BseNI ACTGG 2 cut(s) 381, 471
BshFI GGCC 2 cut(s) 93, 654
BsiSI CCGG 2 cut(s) 78, 731
BslFI GGGAC 1 cut(s) 202
BslI CCNNNNNNNGG 3 cut(s) 100, 417, 566
BsmAI GTCTC 1 cut(s) 244
BsmFI GGGAC 1 cut(s) 202
BsmI GAATGC 1 cut(s) 711
BsnI GGCC 2 cut(s) 93, 654
Bso31I GGTCTC 1 cut(s) 244
Bsp13I TCCGGA 1 cut(s) 730
Bsp143I GATC 5 cut(s) 7, 15, 119, 391, 619
BspACI CCGC 2 cut(s) 90, 775
BspANI GGCC 2 cut(s) 93, 654
BspCNI CTCAG 2 cut(s) 357, 586
BspEI TCCGGA 1 cut(s) 730
BspPI GGATC 3 cut(s) 127, 399, 614
BspTNI GGTCTC 1 cut(s) 244
BsrI ACTGG 2 cut(s) 381, 471
BssECI CCNNGG 1 cut(s) 115
BssMI GATC 5 cut(s) 7, 15, 119, 391, 619
BssT1I CCWWGG 1 cut(s) 115
BstC8I GCNNGC 1 cut(s) 711
BstDEI CTNAG 2 cut(s) 365, 594
BstENI CCTNNNNNAGG 1 cut(s) 564
BstF5I GGATG 3 cut(s) 340, 655, 727
BstKTI GATC 5 cut(s) 10, 18, 122, 394, 622
BstMAI GTCTC 1 cut(s) 244
BstMBI GATC 5 cut(s) 7, 15, 119, 391, 619
BstMWI GCNNNNNNNGC 4 cut(s) 51, 90, 153, 781
BstV2I GAAGAC 2 cut(s) 54, 376
BstX2I RGATCY 2 cut(s) 15, 619
BstYI RGATCY 2 cut(s) 15, 619
BsuRI GGCC 2 cut(s) 93, 654
BtsCI GGATG 3 cut(s) 340, 655, 727
Cac8I GCNNGC 1 cut(s) 711
CaiI CAGNNNCTG 1 cut(s) 509
Csp6I GTAC 3 cut(s) 210, 408, 778
CviAII CATG 1 cut(s) 271
CviQI GTAC 3 cut(s) 210, 408, 778
DdeI CTNAG 2 cut(s) 365, 594
DpnI GATC 5 cut(s) 9, 17, 121, 393, 621
DpnII GATC 5 cut(s) 7, 15, 119, 391, 619
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
Eco130I CCWWGG 1 cut(s) 115
Eco31I GGTCTC 1 cut(s) 244
EcoNI CCTNNNNNAGG 1 cut(s) 564
EcoRI GAATTC 1 cut(s) 266
EcoT14I CCWWGG 1 cut(s) 115
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 1 cut(s) 274
FaiI YATR 9 cut(s) 39, 226, 228, 272, 307, 353, 586, 588, 684
FaqI GGGAC 1 cut(s) 202
FatI CATG 1 cut(s) 270
FauNDI CATATG 2 cut(s) 226, 586
FbaI TGATCA 1 cut(s) 7
FblI GTMKAC 1 cut(s) 763
Fnu4HI GCNGC 1 cut(s) 91
FokI GGATG 3 cut(s) 347, 662, 714
Fsp4HI GCNGC 1 cut(s) 91
FspBI CTAG 2 cut(s) 116, 239
GluI GCNGC 1 cut(s) 91
GsuI CTGGAG 2 cut(s) 122, 452
HaeIII GGCC 2 cut(s) 93, 654
HapII CCGG 2 cut(s) 78, 731
Hin1II CATG 1 cut(s) 274
HincII GTYRAC 1 cut(s) 67
HindII GTYRAC 1 cut(s) 67
HindIII AAGCTT 1 cut(s) 131
HinfI GANTC 5 cut(s) 413, 506, 553, 581, 765
HpaII CCGG 2 cut(s) 78, 731
HphI GGTGA 1 cut(s) 711
Hpy166II GTNNAC 3 cut(s) 67, 166, 764
Hpy188I TCNGA 4 cut(s) 201, 505, 511, 770
Hpy188III TCNNGA 3 cut(s) 101, 215, 731
Hpy8I GTNNAC 3 cut(s) 67, 166, 764
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 1 cut(s) 682
HpyCH4V TGCA 5 cut(s) 274, 659, 665, 676, 713
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 90, 153, 781
HpyF3I CTNAG 2 cut(s) 365, 594
Hsp92II CATG 1 cut(s) 274
Kpn2I TCCGGA 1 cut(s) 730
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 5 cut(s) 7, 15, 119, 391, 619
LmnI GCTCC 1 cut(s) 480
LpnPI CCDG 9 cut(s) 86, 91, 362, 402, 416, 452, 552, 723, 744
LweI GCATC 3 cut(s) 40, 580, 652
MaeI CTAG 2 cut(s) 116, 239
MalI GATC 5 cut(s) 9, 17, 121, 393, 621
MboI GATC 5 cut(s) 7, 15, 119, 391, 619
MboII GAAGA 5 cut(s) 59, 139, 381, 565, 749
MflI RGATCY 2 cut(s) 15, 619
MluCI AATT 7 cut(s) 195, 266, 275, 614, 678, 714, 740
MlyI GAGTC 3 cut(s) 407, 590, 759
MmeI TCCRAC 2 cut(s) 280, 324
MnlI CCTC 5 cut(s) 158, 188, 256, 459, 567
MroI TCCGGA 1 cut(s) 730
MslI CAYNNNNRTG 1 cut(s) 36
MspI CCGG 2 cut(s) 78, 731
Mva1269I GAATGC 1 cut(s) 711
MwoI GCNNNNNNNGC 4 cut(s) 51, 90, 153, 781
NdeI CATATG 2 cut(s) 226, 586
NdeII GATC 5 cut(s) 7, 15, 119, 391, 619
NlaIII CATG 1 cut(s) 274
PctI GAATGC 1 cut(s) 711
PfeI GAWTC 2 cut(s) 506, 553
PkrI GCNGC 1 cut(s) 92
PleI GAGTC 3 cut(s) 407, 589, 759
PpsI GAGTC 3 cut(s) 407, 589, 759
PstNI CAGNNNCTG 1 cut(s) 509
PsuI RGATCY 2 cut(s) 15, 619
RsaI GTAC 3 cut(s) 211, 409, 779
RsaNI GTAC 3 cut(s) 210, 408, 778
RseI CAYNNNNRTG 1 cut(s) 36
SatI GCNGC 1 cut(s) 91
Sau3AI GATC 5 cut(s) 7, 15, 119, 391, 619
SchI GAGTC 3 cut(s) 407, 590, 759
SfaNI GCATC 3 cut(s) 40, 580, 652
SmiMI CAYNNNNRTG 1 cut(s) 36
Sse9I AATT 7 cut(s) 195, 266, 275, 614, 678, 714, 740
SsiI CCGC 2 cut(s) 90, 775
SspI AATATT 1 cut(s) 500
SspMI CTAG 2 cut(s) 116, 239
StyI CCWWGG 1 cut(s) 115
TaqI TCGA 1 cut(s) 749
TasI AATT 7 cut(s) 195, 266, 275, 614, 678, 714, 740
TauI GCSGC 1 cut(s) 93
TfiI GAWTC 2 cut(s) 506, 553
TspDTI ATGAA 1 cut(s) 259
XagI CCTNNNNNAGG 1 cut(s) 564
XapI RAATTY 4 cut(s) 195, 266, 678, 740
XmaJI CCTAGG 1 cut(s) 115
XmiI GTMKAC 1 cut(s) 763
XspI CTAG 2 cut(s) 116, 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.