Rh4AG393500

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000875
Physical Location & Seq
Reverse (-)
69943685 .. 69944598
914 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG393500.1

Sequence Viewer

Length: 822 bp
ATGGCTGGAGAAATTCCTGTGAAGAAGTTTATTTTGTGTGGTGGTGATGATGATGGCGATGATGACAAATATTACGAAGACATGGGAAGTGAATGTTGCCTTTCACTAGATCAGCTGACTTTAGAACCCAGAAGAAAGAAGCTTCTTGTTTTTGATCTGAATGGGTTACTAGTCTACAGGGTTTATTGTTCCAACAAGCCAGAATTTCCAAGTGATCGTACACCGGATGGGAGATTTGGAAACCATCTAGTTTTCAAAAGACCTTTTGCTGAGGAGTTTGTGCAATTTTGCCTTGAAAGATTTGAGATCGGAATATGGTCTTCTGCCGTAGAGAAAAACGTTGATGCTGTCTTAGATTGTATGATGGCAAATCAGAGAAATAGACTATTATTTGTGTGGGGTCAATCTCAATGTACAGATTCTGGGTTCAAGTCCTTGGAGAAAAGAATGAAGCCTCTATTTTTCAAAGAACTGAAGAAAGTGTGGTATCATTTCGGCAGAAAGTATTCTGAATGTGATACTTTGTTGATTGATGATCAACCATACAAGGCCCTACTCAATCCTGCTCACACAGGGATTTTTTTGGAATCCTACAATCCTGACAATGCTAGTGATAATTTATTGGATCCAAATGGACAGCTAGGGGTGTATTTGGATGGCCTTGCGGCTGCGACTAATGCTCAGGTTTATGTGAAGGAGAACCCTATTGGAGTGCCTGCAATTTCATCAGATCATCCTGATTGGAATTTCTACTCTGAAGTCCTTCGAGGGATTAAGGGAGAAAGTGAGGAAAAGATTACGCCTCAGCAACAAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

273

Amino Acids

31.14

Weight (kDa)

4.89

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 47 - 218 1.7e-20 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AciI CCGC 1 cut(s) 665
AclI AACGTT 1 cut(s) 339
AclWI GGATC 2 cut(s) 620, 633
AcsI RAATTY 3 cut(s) 12, 203, 745
AcuI CTGAAG 2 cut(s) 494, 777
AfaI GTAC 2 cut(s) 220, 415
AgsI TTSAA 4 cut(s) 256, 296, 430, 466
AhlI ACTAGT 1 cut(s) 169
AluBI AGCT 3 cut(s) 115, 142, 640
AluI AGCT 3 cut(s) 115, 142, 640
AlwI GGATC 2 cut(s) 620, 633
AlwNI CAGNNNCTG 1 cut(s) 422
AoxI GGCC 2 cut(s) 549, 658
ApeKI GCWGC 1 cut(s) 668
ApoI RAATTY 3 cut(s) 12, 203, 745
Asp700I GAANNNNTTC 2 cut(s) 505, 762
AspS9I GGNCC 1 cut(s) 550
AsuHPI GGTGA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 625
BbsI GAAGAC 2 cut(s) 84, 312
BbvCI CCTCAGC 2 cut(s) 270, 804
BbvI GCAGC 1 cut(s) 655
BccI CCATC 5 cut(s) 47, 221, 252, 358, 650
BceAI ACGGC 1 cut(s) 311
BclI TGATCA 1 cut(s) 535
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 5 cut(s) 107, 170, 248, 609, 641
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 2 cut(s) 666, 669
BlsI GCNGC 2 cut(s) 667, 670
BmgT120I GGNCC 1 cut(s) 550
BmiI GGNNCC 1 cut(s) 627
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 2 cut(s) 84, 312
BpmI CTGGAG 1 cut(s) 27
Bpu10I CCTNAGC 3 cut(s) 270, 681, 804
BsaJI CCNNGG 1 cut(s) 435
BsaWI WCCGGW 1 cut(s) 223
BseDI CCNNGG 1 cut(s) 435
BseGI GGATG 3 cut(s) 232, 661, 733
BseMII CTCAG 3 cut(s) 261, 695, 818
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 655
BshFI GGCC 2 cut(s) 551, 660
BsiSI CCGG 1 cut(s) 224
BsnI GGCC 2 cut(s) 551, 660
Bsp1407I TGTACA 1 cut(s) 413
Bsp143I GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BspACI CCGC 1 cut(s) 665
BspANI GGCC 2 cut(s) 551, 660
BspCNI CTCAG 3 cut(s) 262, 694, 817
BspLI GGNNCC 1 cut(s) 627
BspPI GGATC 2 cut(s) 620, 633
BsrGI TGTACA 1 cut(s) 413
BssECI CCNNGG 1 cut(s) 435
BssMI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BssT1I CCWWGG 1 cut(s) 435
BstAUI TGTACA 1 cut(s) 413
BstC8I GCNNGC 1 cut(s) 717
BstDEI CTNAG 4 cut(s) 270, 352, 681, 804
BstF5I GGATG 3 cut(s) 232, 661, 733
BstKTI GATC 7 cut(s) 112, 157, 217, 309, 538, 628, 733
BstMBI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
BstMWI GCNNNNNNNGC 1 cut(s) 677
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 655
BstV2I GAAGAC 2 cut(s) 84, 312
BstX2I RGATCY 1 cut(s) 625
BstYI RGATCY 1 cut(s) 625
BsuRI GGCC 2 cut(s) 551, 660
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 232, 661, 733
Cac8I GCNNGC 1 cut(s) 717
CaiI CAGNNNCTG 1 cut(s) 422
Cfr13I GGNCC 1 cut(s) 550
Csp6I GTAC 2 cut(s) 219, 414
CviAII CATG 1 cut(s) 82
CviJI RGCY 9 cut(s) 5, 115, 142, 199, 454, 551, 640, 660, 668
CviKI_1 RGCY 9 cut(s) 5, 115, 142, 199, 454, 551, 640, 660, 668
CviQI GTAC 2 cut(s) 219, 414
DdeI CTNAG 4 cut(s) 270, 352, 681, 804
DpnI GATC 7 cut(s) 111, 156, 216, 308, 537, 627, 732
DpnII GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
Eco130I CCWWGG 1 cut(s) 435
Eco57I CTGAAG 2 cut(s) 494, 777
EcoO109I RGGNCCY 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 435
ErhI CCWWGG 1 cut(s) 435
FaeI CATG 1 cut(s) 85
FaiI YATR 5 cut(s) 83, 316, 362, 544, 690
FatI CATG 1 cut(s) 81
FbaI TGATCA 1 cut(s) 535
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 666, 669
FokI GGATG 3 cut(s) 239, 668, 720
Fsp4HI GCNGC 2 cut(s) 666, 669
FspBI CTAG 5 cut(s) 107, 170, 248, 609, 641
GluI GCNGC 2 cut(s) 666, 669
GsuI CTGGAG 1 cut(s) 27
HaeIII GGCC 2 cut(s) 551, 660
HapII CCGG 1 cut(s) 224
Hin1II CATG 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 3 cut(s) 419, 587, 815
HpaII CCGG 1 cut(s) 224
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 2 cut(s) 175, 221
Hpy188I TCNGA 6 cut(s) 159, 311, 375, 511, 730, 757
Hpy188III TCNNGA 3 cut(s) 599, 737, 819
Hpy8I GTNNAC 2 cut(s) 175, 221
HpyAV CCTTC 2 cut(s) 688, 773
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 2 cut(s) 283, 719
HpyF10VI GCNNNNNNNGC 1 cut(s) 677
HpyF3I CTNAG 4 cut(s) 270, 352, 681, 804
HpySE526I ACGT 1 cut(s) 339
Hsp92II CATG 1 cut(s) 85
Ksp22I TGATCA 1 cut(s) 535
Kzo9I GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
Lsp1109I GCAGC 1 cut(s) 655
LweI GCATC 1 cut(s) 334
MaeI CTAG 5 cut(s) 107, 170, 248, 609, 641
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 165
MalI GATC 7 cut(s) 111, 156, 216, 308, 537, 627, 732
MboI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
MboII GAAGA 5 cut(s) 34, 89, 144, 312, 487
MflI RGATCY 1 cut(s) 625
MluCI AATT 6 cut(s) 12, 203, 284, 616, 720, 745
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 5 cut(s) 265, 465, 761, 781, 813
MroXI GAANNNNTTC 2 cut(s) 505, 762
MseI TTAA 1 cut(s) 774
MspA1I CMGCKG 1 cut(s) 115
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 1 cut(s) 677
NdeII GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
NlaIII CATG 1 cut(s) 85
NlaIV GGNNCC 1 cut(s) 627
PdmI GAANNNNTTC 2 cut(s) 505, 762
PfeI GAWTC 2 cut(s) 419, 587
PkrI GCNGC 2 cut(s) 667, 670
Psp1406I AACGTT 1 cut(s) 339
PspN4I GGNNCC 1 cut(s) 627
PspPI GGNCC 1 cut(s) 550
PstNI CAGNNNCTG 1 cut(s) 422
PsuI RGATCY 1 cut(s) 625
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 2 cut(s) 220, 415
RsaNI GTAC 2 cut(s) 219, 414
SaqAI TTAA 1 cut(s) 774
SatI GCNGC 2 cut(s) 666, 669
Sau3AI GATC 7 cut(s) 109, 154, 214, 306, 535, 625, 730
Sau96I GGNCC 1 cut(s) 550
SetI ASST 6 cut(s) 117, 144, 265, 342, 642, 687
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 175
SpeI ACTAGT 1 cut(s) 169
Sse9I AATT 6 cut(s) 12, 203, 284, 616, 720, 745
SsiI CCGC 1 cut(s) 665
SspI AATATT 1 cut(s) 71
SspMI CTAG 5 cut(s) 107, 170, 248, 609, 641
StyI CCWWGG 1 cut(s) 435
TaiI ACGT 1 cut(s) 342
TaqI TCGA 1 cut(s) 766
TasI AATT 6 cut(s) 12, 203, 284, 616, 720, 745
TatI WGTACW 1 cut(s) 413
TauI GCSGC 1 cut(s) 668
TfiI GAWTC 2 cut(s) 419, 587
Tru1I TTAA 1 cut(s) 774
Tru9I TTAA 1 cut(s) 774
TseI GCWGC 1 cut(s) 668
TspDTI ATGAA 2 cut(s) 464, 714
XapI RAATTY 3 cut(s) 12, 203, 745
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 505, 762
XspI CTAG 5 cut(s) 107, 170, 248, 609, 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.