Rroxscaffold_6G00409040

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000875
Physical Location & Seq
Reverse (-)
31564130 .. 31565305
1176 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409040.1

Sequence Viewer

Length: 768 bp
ATGACCCCGAAAGCCCGAAAACCCACACGTCGTGGCATAGCGACGAGTTTTGTTCTTGGTGCAATTCCCTTTCCGGAAAGTTATGGCACGATCGAATCGGACACCGAATGCGAAAGTTACAATAGTAACTTGGGTTTTCCTCCTTTTGCACGATCGAACCGTTCTTCAATTCGGATCGCCATTTGTTCTACATCGATCTTGATAACAGTTTACAAGAGGCCTTACCGTGAAGAATTTATGAAGTTTTGTCCGGAAAGATTTGAAGTGGGAATATGGTCTTCTCGCAATGGAATGGTACTTGGACAATGCCTTGGATTGTGCGATGAAAGGGATCAAGTGGAGTGTACTAATTCAGGGTTCAAAACCTTGGAGAACCAAAAGAAGCCCATCTTTCTGAAAGAGTTGAAGAAAATATGGGAAAACAAGGGTTTCAAAGCCTCACTTCCATCTTCCATAGGGAAATACTCTTCATTGAACACCTTGTTAATTGATGATAAGGCTTACAAGGCCCTACTAAATCCCGCTCACACAACCATCTTTCCTCCTGAATACAAGGTTGACCAAGTTGATGACAAGGCTTTAGGTCTTAATGGTGAGTTGAGGCTTTACTTGGAAGGACTTGCAAATGTCGATGATGTTACTTCTTATGTTAAAGATCATCCATTTGGACAGCCAGTGATAATAACTACTCACTCGATTGGGATTTCTATTCAAAGATCCTTACTCATTTCCGTAAAGATTAAGCTACTACAAAAGCTCATCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

255

Amino Acids

28.66

Weight (kDa)

9.03

Isoelectric Point (pI)

35.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 57 - 213 2.5e-13 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 524
AccIII TCCGGA 2 cut(s) 73, 250
AciI CCGC 1 cut(s) 522
AclWI GGATC 3 cut(s) 182, 339, 711
AcsI RAATTY 1 cut(s) 233
AdeI CACNNNGTG 1 cut(s) 32
AfaI GTAC 2 cut(s) 297, 346
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 7 cut(s) 168, 263, 361, 406, 433, 475, 713
AjiI CACGTC 1 cut(s) 29
AluBI AGCT 2 cut(s) 745, 757
AluI AGCT 2 cut(s) 745, 757
AlwI GGATC 3 cut(s) 182, 339, 711
Aor13HI TCCGGA 2 cut(s) 73, 250
AoxI GGCC 2 cut(s) 218, 507
ApoI RAATTY 1 cut(s) 233
AspS9I GGNCC 1 cut(s) 508
AsuHPI GGTGA 1 cut(s) 605
BbsI GAAGAC 1 cut(s) 270
BccI CCATC 3 cut(s) 395, 454, 542
BmgBI CACGTC 1 cut(s) 29
BmgT120I GGNCC 1 cut(s) 508
BpiI GAAGAC 1 cut(s) 270
Bsa29I ATCGAT 1 cut(s) 194
BsaJI CCNNGG 2 cut(s) 310, 366
BsaWI WCCGGW 2 cut(s) 73, 250
Bse1I ACTGG 1 cut(s) 674
Bse3DI GCAATG 1 cut(s) 292
BseAI TCCGGA 2 cut(s) 73, 250
BseCI ATCGAT 1 cut(s) 194
BseDI CCNNGG 2 cut(s) 310, 366
BseGI GGATG 1 cut(s) 658
BseMI GCAATG 1 cut(s) 292
BseNI ACTGG 1 cut(s) 674
Bsh1285I CGRYCG 2 cut(s) 93, 155
BshFI GGCC 2 cut(s) 220, 509
BshVI ATCGAT 1 cut(s) 194
BsiEI CGRYCG 2 cut(s) 93, 155
BsiSI CCGG 2 cut(s) 74, 251
BsmI GAATGC 1 cut(s) 113
BsnI GGCC 2 cut(s) 220, 509
Bsp13I TCCGGA 2 cut(s) 73, 250
Bsp143I GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
BspACI CCGC 1 cut(s) 522
BspANI GGCC 2 cut(s) 220, 509
BspDI ATCGAT 1 cut(s) 194
BspEI TCCGGA 2 cut(s) 73, 250
BspPI GGATC 3 cut(s) 182, 339, 711
BsrBI CCGCTC 1 cut(s) 524
BsrDI GCAATG 1 cut(s) 292
BsrI ACTGG 1 cut(s) 674
BssECI CCNNGG 2 cut(s) 310, 366
BssMI GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
BssT1I CCWWGG 2 cut(s) 310, 366
Bst4CI ACNGT 3 cut(s) 161, 208, 227
Bst6I CTCTTC 1 cut(s) 472
BstF5I GGATG 1 cut(s) 658
BstKTI GATC 7 cut(s) 93, 155, 177, 198, 334, 658, 719
BstMBI GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
BstMCI CGRYCG 2 cut(s) 93, 155
BstMWI GCNNNNNNNGC 1 cut(s) 506
BstV2I GAAGAC 1 cut(s) 270
BstX2I RGATCY 1 cut(s) 716
BstYI RGATCY 1 cut(s) 716
Bsu15I ATCGAT 1 cut(s) 194
BsuRI GGCC 2 cut(s) 220, 509
BsuTUI ATCGAT 1 cut(s) 194
BtgZI GCGATG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 29
BtsCI GGATG 1 cut(s) 658
BtsIMutI CAGTG 1 cut(s) 681
Cfr13I GGNCC 1 cut(s) 508
ClaI ATCGAT 1 cut(s) 194
Csp6I GTAC 2 cut(s) 296, 345
CviQI GTAC 2 cut(s) 296, 345
DpnI GATC 7 cut(s) 92, 154, 176, 197, 333, 657, 718
DpnII GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
DraIII CACNNNGTG 1 cut(s) 32
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco130I CCWWGG 2 cut(s) 310, 366
Eco147I AGGCCT 1 cut(s) 220
EcoO109I RGGNCCY 1 cut(s) 508
EcoT14I CCWWGG 2 cut(s) 310, 366
ErhI CCWWGG 2 cut(s) 310, 366
FaiI YATR 7 cut(s) 38, 84, 239, 274, 415, 455, 648
FalI AAGNNNNNCTT 4 cut(s) 374, 406, 426, 458
FauI CCCGC 1 cut(s) 529
FokI GGATG 1 cut(s) 645
HaeIII GGCC 2 cut(s) 220, 509
HapII CCGG 2 cut(s) 74, 251
HincII GTYRAC 1 cut(s) 559
HindII GTYRAC 1 cut(s) 559
HinfI GANTC 1 cut(s) 95
HpaII CCGG 2 cut(s) 74, 251
HphI GGTGA 1 cut(s) 605
Hpy166II GTNNAC 3 cut(s) 211, 345, 559
Hpy188I TCNGA 3 cut(s) 100, 174, 396
Hpy188III TCNNGA 4 cut(s) 74, 199, 251, 545
Hpy8I GTNNAC 3 cut(s) 211, 345, 559
Hpy99I CGWCG 2 cut(s) 33, 46
HpyAV CCTTC 1 cut(s) 608
HpyCH4III ACNGT 3 cut(s) 161, 208, 227
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 3 cut(s) 62, 149, 623
HpyF10VI GCNNNNNNNGC 1 cut(s) 506
HpySE526I ACGT 1 cut(s) 28
Kpn2I TCCGGA 2 cut(s) 73, 250
Kzo9I GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
LpnPI CCDG 5 cut(s) 87, 264, 339, 558, 687
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 3 cut(s) 116, 125, 637
MalI GATC 7 cut(s) 92, 154, 176, 197, 333, 657, 718
MbiI CCGCTC 1 cut(s) 524
MboI GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
MboII GAAGA 6 cut(s) 156, 242, 270, 418, 441, 459
MflI RGATCY 1 cut(s) 716
MluCI AATT 6 cut(s) 63, 168, 233, 349, 486, 763
MnlI CCTC 5 cut(s) 150, 210, 448, 552, 594
MroI TCCGGA 2 cut(s) 73, 250
MseI TTAA 5 cut(s) 485, 588, 651, 741, 766
MspI CCGG 2 cut(s) 74, 251
Mva1269I GAATGC 1 cut(s) 113
MwoI GCNNNNNNNGC 1 cut(s) 506
NdeII GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
PceI AGGCCT 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 157
PctI GAATGC 1 cut(s) 113
PfeI GAWTC 1 cut(s) 95
Ple19I CGATCG 2 cut(s) 93, 155
PspPI GGNCC 1 cut(s) 508
PsuI RGATCY 1 cut(s) 716
PvuI CGATCG 2 cut(s) 93, 155
RsaI GTAC 2 cut(s) 297, 346
RsaNI GTAC 2 cut(s) 296, 345
SaqAI TTAA 5 cut(s) 485, 588, 651, 741, 766
Sau3AI GATC 7 cut(s) 90, 152, 174, 195, 331, 655, 716
Sau96I GGNCC 1 cut(s) 508
SetI ASST 7 cut(s) 31, 368, 482, 558, 586, 747, 759
Sse9I AATT 6 cut(s) 63, 168, 233, 349, 486, 763
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 1 cut(s) 522
StuI AGGCCT 1 cut(s) 220
StyI CCWWGG 2 cut(s) 310, 366
TaaI ACNGT 3 cut(s) 161, 208, 227
TaiI ACGT 1 cut(s) 31
TaqI TCGA 5 cut(s) 93, 155, 194, 630, 695
TasI AATT 6 cut(s) 63, 168, 233, 349, 486, 763
TatI WGTACW 1 cut(s) 344
TfiI GAWTC 1 cut(s) 95
Tru1I TTAA 5 cut(s) 485, 588, 651, 741, 766
Tru9I TTAA 5 cut(s) 485, 588, 651, 741, 766
TscAI CASTG 1 cut(s) 681
TspDTI ATGAA 3 cut(s) 254, 339, 459
TspGWI ACGGA 1 cut(s) 721
TspRI CASTG 1 cut(s) 681
XapI RAATTY 1 cut(s) 233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.