FvH4_6g18040

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000875
Physical Location & Seq
Forward (+)
11841967 .. 11850173
8207 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g18040.t2

Sequence Viewer

Length: 2298 bp
ATGATGGGGCGAAATCTAACACATCATTCCAAACCTCCCGTACAGGGCCATGAGTCTCCTCATAGTAGGAGTCCGTCTAGGAAGAGTCAATATCGAAGAGAAAGATCACCTACCCGTCACAGAGATTCCCACAGAGGCAGTTCTCCATCAAGTGAGAAATATTCAATTCGCCTCAAGTTTCCCAAGCATGCAAGGTCACCCTCTCCTTTCTCTCCTTTTCCCCGGACAAAACGGTTAAGAAGAGCTGAAGCTGGAGAAGTGCTTGAGACAGAACAGGAAAGGATCAATGGTCGGGAGATCAGTTGGAATTTACAGGAAGGTGGTTCTAATGGATATGTTAGAAGAACGGAGAATTTTTCTGGAAGGAATGATACTCATGGTAAATCTTCAAGATCAAGGCATGGTAGTTCTCCATCAGATAGAGAGTGCAGGAGTAGACATAGACACTCGCCTCAACATGCTTGTGATACAAGAGATAGGGAGAAAGTAACTGAGAGCCGTGACAGAAATCATACTAGAGGGAATGATAGAAGAATGTATAGGGAAAATGAGACTGATAGAATGGAAAGAATGACAGCAAAAGACCGTACGGGTTATCTGTCTTCAAGCTCGAGGCATGATATTTCTCCATCAGATCAACACCGCAGGAGTAGACTTAGATCTTACTCTCCTCAACATACTTGTGATACAAGACATGGGGAGAAAGTGATGGAGGACCGTGAAAGAAATCATAGCAGAGAGAATCGTAGAAGCTTGCATATGGAGAATGACAATGAAAGAATGGAGAGAATCACAGCAAAAGACAGTACTAGTCATAGGTCTTCAAGATCAACGCATGGTTCTCCATCAGATCGAAACAGCAGGAGTAGATATAGATCTCTTTTACCTCAACATACTGGTGATACAAGAGACAATGAGAAATTAATTGAGGGCAGTGAAACAATTTATAGCAGAGAAAATCATAGAAGTATAGATAGGAAAAATGATACTGAAAGATCGGAGAGATTGACAGCAGAAGAGAATACTGGTCATAGGTCTTCAAGATCAAGGCATGTTACTTCTCCATCAGATCGACACTGCAGTAGTAGACATAGATATCACTCACCTCAACATGCTGGTGATACAAGAGATGAGGAGAAAATAATTGGTGGCCGTCAAAGATTTCATAGGAGAGAGAGTGATAGCAGTATGCACAGGGAAAAGCATTCAGATAGGGAAATTCAGACTGAAAAAATGGAGAGAAGGACAGCAAAAGACAGTTCTGTTCATCGGTCTTTAAGATCAAGAAAGGGGCAATCTACTTCTCCGTTGAATGGATACCACAAAAACAAACACCGAACTCATTTTTCTGAACAAGCTGCAGATACCAGAGCTCACAATAAGGTGCCCAAGTTCATGGAGGCTAAAGCTAGGATTGATGAGAATGATTCAGTAGCTATGATGAGGGCTGCTGAGGAAGTCTTGGAATCTCAGAAAAAGCAAAAGTCTTCATTTGAGTTATTTGGAAAGCTTGCTGTGGAAACCAGTAGATTCAGGGGAGATGGCCCTAAGAAGAAGCTTATATCGAGTAATGCCAATGGTGATGAAGACTATGATAATGGAAGAACACGAAGCATATGTAGGCTGAATTTAGCACCAAGAAAAAAGAAGCTTCTCGTGTTCGATCTCAACGGCTTACTGGTCTACAGAGTTTTTTGTTTTAACAAGGGTGGTATTCCAAAGGATCGTACTTCTGATGGGAGATGTGGAAACCATCTAGTTTATAAAAGACCTTTTGCTGAGGAGTTTATGCTATTCTGCCTCGAAAGATTTGAAGTTGGAATATGGACTTCTGCTCTGGAGAAAAATGTTGATGGTGTCTTGGATTGTGTGATGGAAAAGCTGAGAAGCAGACTCGTATTTGTGTGGGATCAGCATCGATGTACTGATTCTGGGTACAAGTCCCTGGAAACGAAAATGAAGCCTCTATATCTCAAAGAAGTGAAGAAAGTATGGGATCATTTTGAGGGGAAGTTTTCTGCATCGGATACTCTATTGATTGATGATCAGCCATACAAGGCCCTTCTCAATCCTCCTTACACAGGGATTTTCCTGGATCCTTACATTCCCGACAATGGTAGTGACAATGCTTTAGATCCAAAGAAAGAATTGGGGGAGTATTTGGCTGGCCTTGCGGTTGCAGATGATGTTCAGCTTTATGTAAAGGAAAATCCCTTTGGCCAGCCTGCGATTTCATCTGAACATCCTAATTGGAATTTTTACTCTGCTGTGCTTCAAAAACTTGGAAAATATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

766

Amino Acids

88.94

Weight (kDa)

9.79

Isoelectric Point (pI)

62.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1764
AccB1I GGYRCC 1 cut(s) 1384
AccI GTMKAC 4 cut(s) 436, 652, 1087, 1683
AciI CCGC 2 cut(s) 643, 2174
AclWI GGATC 7 cut(s) 290, 1731, 1917, 2004, 2090, 2103, 2129
AcoI YGGCCR 2 cut(s) 1150, 2218
AcsI RAATTY 5 cut(s) 307, 352, 1218, 1627, 2254
AcuI CTGAAG 1 cut(s) 267
AfaI GTAC 6 cut(s) 42, 589, 808, 1729, 1924, 1937
AfiI CCNNNNNNNGG 4 cut(s) 44, 1313, 2081, 2114
AgsI TTSAA 8 cut(s) 165, 390, 606, 825, 1041, 1312, 1814, 2276
AhlI ACTAGT 1 cut(s) 809
AjnI CCWGG 2 cut(s) 1944, 2091
AjuI GAANNNNNNNTTGG 2 cut(s) 2199, 2231
Alw21I GWGCWC 1 cut(s) 1375
Alw26I GTCTC 4 cut(s) 60, 260, 545, 903
AlwI GGATC 7 cut(s) 290, 1731, 1917, 2004, 2090, 2103, 2129
Ama87I CYCGRG 1 cut(s) 610
AoxI GGCC 6 cut(s) 46, 1150, 1543, 2058, 2167, 2218
ApeKI GCWGC 2 cut(s) 1358, 1448
ApoI RAATTY 5 cut(s) 307, 352, 1218, 1627, 2254
AseI ATTAAT 1 cut(s) 923
AspS9I GGNCC 4 cut(s) 46, 715, 1544, 2059
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 6 cut(s) 99, 189, 911, 1095, 1130, 1592
AvaI CYCGRG 1 cut(s) 610
AvaII GGWCC 1 cut(s) 715
BaeGI GKGCMC 1 cut(s) 1389
BalI TGGCCA 1 cut(s) 2220
BamHI GGATCC 1 cut(s) 2095
BanI GGYRCC 1 cut(s) 1384
BanII GRGCYC 1 cut(s) 1375
BarI GAAGNNNNNNTAC 2 cut(s) 355, 387
BauI CACGAG 1 cut(s) 1655
BbsI GAAGAC 5 cut(s) 594, 813, 1029, 1479, 1593
Bbv12I GWGCWC 1 cut(s) 1375
BbvCI CCTCAGC 2 cut(s) 1452, 1779
BbvI GCAGC 2 cut(s) 1345, 1435
BceAI ACGGC 3 cut(s) 483, 1137, 1687
BciT130I CCWGG 2 cut(s) 1946, 2093
BciVI GTATCC 2 cut(s) 1310, 2020
BclI TGATCA 1 cut(s) 2044
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 4 cut(s) 60, 260, 545, 903
BcuI ACTAGT 1 cut(s) 809
BfaI CTAG 5 cut(s) 78, 516, 810, 1410, 1757
BfmI CTRYAG 4 cut(s) 1078, 1359, 1684, 2294
BfuI GTATCC 2 cut(s) 1310, 2020
BglII AGATCT 2 cut(s) 659, 875
BisI GCNGC 2 cut(s) 1359, 1449
BlsI GCNGC 2 cut(s) 1360, 1450
BmcAI AGTACT 1 cut(s) 808
Bme1390I CCNGG 3 cut(s) 223, 1946, 2093
Bme18I GGWCC 1 cut(s) 715
BmeT110I CYCGRG 1 cut(s) 610
BmgT120I GGNCC 4 cut(s) 46, 715, 1544, 2059
BmiI GGNNCC 2 cut(s) 1386, 2097
BmrFI CCNGG 3 cut(s) 223, 1946, 2093
BmsI GCATC 2 cut(s) 1924, 2030
BpiI GAAGAC 5 cut(s) 594, 813, 1029, 1479, 1593
BpmI CTGGAG 2 cut(s) 273, 1859
Bpu10I CCTNAGC 2 cut(s) 1452, 1779
BpuEI CTTGAG 2 cut(s) 158, 284
BpuMI CCSGG 1 cut(s) 223
Bsa29I ATCGAT 1 cut(s) 1918
BsaBI GATNNNNATC 2 cut(s) 874, 1914
BsaJI CCNNGG 2 cut(s) 221, 1944
BsaXI ACNNNNNCTCC 2 cut(s) 1162, 1192
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 1313, 2081, 2114
Bse1I ACTGG 4 cut(s) 901, 1030, 1524, 1683
Bse8I GATNNNNATC 2 cut(s) 874, 1914
BseBI CCWGG 2 cut(s) 1946, 2093
BseCI ATCGAT 1 cut(s) 1918
BseDI CCNNGG 2 cut(s) 221, 1944
BseGI GGATG 1 cut(s) 2242
BseJI GATNNNNATC 2 cut(s) 874, 1914
BseLI CCNNNNNNNGG 4 cut(s) 44, 1313, 2081, 2114
BseMII CTCAG 5 cut(s) 483, 1443, 1484, 1770, 1874
BseNI ACTGG 4 cut(s) 901, 1030, 1524, 1683
BseRI GAGGAG 4 cut(s) 48, 660, 1148, 1796
BseSI GKGCMC 1 cut(s) 1389
BseXI GCAGC 2 cut(s) 1345, 1435
BsgI GTGCAG 1 cut(s) 448
BshFI GGCC 6 cut(s) 48, 1152, 1545, 2060, 2169, 2220
BshNI GGYRCC 1 cut(s) 1384
BshVI ATCGAT 1 cut(s) 1918
BsiHKAI GWGCWC 1 cut(s) 1375
BsiHKCI CYCGRG 1 cut(s) 610
BsiSI CCGG 1 cut(s) 223
BsiWI CGTACG 1 cut(s) 587
BslFI GGGAC 1 cut(s) 1927
BslI CCNNNNNNNGG 4 cut(s) 44, 1313, 2081, 2114
BsmAI GTCTC 4 cut(s) 60, 260, 545, 903
BsmFI GGGAC 1 cut(s) 1927
BsmI GAATGC 1 cut(s) 1204
BsnI GGCC 6 cut(s) 48, 1152, 1545, 2060, 2169, 2220
BsoBI CYCGRG 1 cut(s) 610
Bsp1286I GDGCHC 2 cut(s) 1375, 1389
BspACI CCGC 2 cut(s) 643, 2174
BspANI GGCC 6 cut(s) 48, 1152, 1545, 2060, 2169, 2220
BspCNI CTCAG 5 cut(s) 484, 1444, 1483, 1771, 1875
BspDI ATCGAT 1 cut(s) 1918
BspLI GGNNCC 2 cut(s) 1386, 2097
BspMAI CTGCAG 2 cut(s) 1082, 1363
BspPI GGATC 7 cut(s) 290, 1731, 1917, 2004, 2090, 2103, 2129
BspQI GCTCTTC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 1384
BsrI ACTGG 4 cut(s) 901, 1030, 1524, 1683
BssECI CCNNGG 2 cut(s) 221, 1944
BssSI CACGAG 1 cut(s) 1655
Bst2BI CACGAG 1 cut(s) 1655
Bst2UI CCWGG 2 cut(s) 1946, 2093
Bst4CI ACNGT 5 cut(s) 234, 587, 719, 806, 1259
Bst6I CTCTTC 4 cut(s) 77, 91, 235, 1011
BstC8I GCNNGC 6 cut(s) 189, 755, 1512, 2167, 2222, 2226
BstDEI CTNAG 7 cut(s) 492, 656, 1452, 1470, 1548, 1779, 1883
BstEII GGTNACC 1 cut(s) 195
BstENI CCTNNNNNAGG 1 cut(s) 2079
BstF5I GGATG 1 cut(s) 2242
BstMAI GTCTC 4 cut(s) 60, 260, 545, 903
BstMWI GCNNNNNNNGC 1 cut(s) 2171
BstNI CCWGG 2 cut(s) 1946, 2093
BstNSI RCATGY 4 cut(s) 191, 461, 1055, 1115
BstPI GGTNACC 1 cut(s) 195
BstSCI CCNGG 3 cut(s) 221, 1944, 2091
BstSFI CTRYAG 4 cut(s) 1078, 1359, 1684, 2294
BstSLI GKGCMC 1 cut(s) 1389
BstV1I GCAGC 2 cut(s) 1345, 1435
BstV2I GAAGAC 5 cut(s) 594, 813, 1029, 1479, 1593
BstX2I RGATCY 4 cut(s) 659, 875, 2095, 2134
BstXI CCANNNNNNTGG 1 cut(s) 1396
BstYI RGATCY 4 cut(s) 659, 875, 2095, 2134
Bsu15I ATCGAT 1 cut(s) 1918
BsuI GTATCC 2 cut(s) 1310, 2020
BsuRI GGCC 6 cut(s) 48, 1152, 1545, 2060, 2169, 2220
BsuTUI ATCGAT 1 cut(s) 1918
BtsCI GGATG 1 cut(s) 2242
BtsI GCAGTG 2 cut(s) 940, 1075
BtsIMutI CAGTG 2 cut(s) 940, 1075
Cac8I GCNNGC 6 cut(s) 189, 755, 1512, 2167, 2222, 2226
Cfr13I GGNCC 4 cut(s) 46, 715, 1544, 2059
ClaI ATCGAT 1 cut(s) 1918
Csp6I GTAC 6 cut(s) 41, 588, 807, 1728, 1923, 1936
CviQI GTAC 6 cut(s) 41, 588, 807, 1728, 1923, 1936
DdeI CTNAG 7 cut(s) 492, 656, 1452, 1470, 1548, 1779, 1883
EaeI YGGCCR 2 cut(s) 1150, 2218
Eam1104I CTCTTC 4 cut(s) 77, 91, 235, 1011
EarI CTCTTC 4 cut(s) 77, 91, 235, 1011
Ecl136II GAGCTC 1 cut(s) 1373
Eco24I GRGCYC 1 cut(s) 1375
Eco32I GATATC 1 cut(s) 1097
Eco47I GGWCC 1 cut(s) 715
Eco53kI GAGCTC 1 cut(s) 1373
Eco57I CTGAAG 1 cut(s) 267
Eco88I CYCGRG 1 cut(s) 610
Eco91I GGTNACC 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 1373
EcoNI CCTNNNNNAGG 1 cut(s) 2079
EcoO109I RGGNCCY 1 cut(s) 2059
EcoO65I GGTNACC 1 cut(s) 195
EcoRII CCWGG 2 cut(s) 1944, 2091
EcoRV GATATC 1 cut(s) 1097
EcoT38I GRGCYC 1 cut(s) 1375
FaqI GGGAC 1 cut(s) 1927
FauNDI CATATG 2 cut(s) 759, 1616
FbaI TGATCA 1 cut(s) 2044
FblI GTMKAC 4 cut(s) 436, 652, 1087, 1683
Fnu4HI GCNGC 2 cut(s) 1359, 1449
FokI GGATG 1 cut(s) 2229
FriOI GRGCYC 1 cut(s) 1375
Fsp4HI GCNGC 2 cut(s) 1359, 1449
FspBI CTAG 5 cut(s) 78, 516, 810, 1410, 1757
GluI GCNGC 2 cut(s) 1359, 1449
GsuI CTGGAG 2 cut(s) 273, 1859
HaeIII GGCC 6 cut(s) 48, 1152, 1545, 2060, 2169, 2220
HapII CCGG 1 cut(s) 223
HindIII AAGCTT 4 cut(s) 751, 1508, 1556, 1649
HpaII CCGG 1 cut(s) 223
HphI GGTGA 6 cut(s) 99, 189, 911, 1095, 1130, 1592
Hpy166II GTNNAC 4 cut(s) 437, 653, 1088, 1684
Hpy188III TCNNGA 8 cut(s) 293, 360, 390, 825, 1041, 1284, 1838, 2108
Hpy8I GTNNAC 4 cut(s) 437, 653, 1088, 1684
HpyAV CCTTC 4 cut(s) 311, 357, 1236, 2072
HpyCH4III ACNGT 5 cut(s) 234, 587, 719, 806, 1259
HpyCH4V TGCA 8 cut(s) 191, 429, 757, 1080, 1192, 1361, 2021, 2180
HpyF10VI GCNNNNNNNGC 1 cut(s) 2171
HpyF3I CTNAG 7 cut(s) 492, 656, 1452, 1470, 1548, 1779, 1883
Ksp22I TGATCA 1 cut(s) 2044
LguI GCTCTTC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 1345, 1435
LweI GCATC 2 cut(s) 1924, 2030
MaeI CTAG 5 cut(s) 78, 516, 810, 1410, 1757
MaeIII GTNAC 6 cut(s) 116, 195, 487, 500, 1054, 2120
MflI RGATCY 4 cut(s) 659, 875, 2095, 2134
MhlI GDGCHC 2 cut(s) 1375, 1389
MlsI TGGCCA 1 cut(s) 2220
MluNI TGGCCA 1 cut(s) 2220
MlyI GAGTC 4 cut(s) 62, 79, 94, 1887
MmeI TCCRAC 2 cut(s) 284, 1798
Mox20I TGGCCA 1 cut(s) 2220
MscI TGGCCA 1 cut(s) 2220
MseI TTAA 4 cut(s) 236, 923, 1277, 1701
MslI CAYNNNNRTG 4 cut(s) 462, 681, 897, 1116
Msp20I TGGCCA 1 cut(s) 2220
MspI CCGG 1 cut(s) 223
MspR9I CCNGG 3 cut(s) 223, 1946, 2093
Mva1269I GAATGC 1 cut(s) 1204
MvaI CCWGG 2 cut(s) 1946, 2093
MwoI GCNNNNNNNGC 1 cut(s) 2171
NciI CCSGG 1 cut(s) 223
NdeI CATATG 2 cut(s) 759, 1616
NlaIV GGNNCC 2 cut(s) 1386, 2097
NmuCI GTSAC 4 cut(s) 116, 195, 500, 2120
NspI RCATGY 4 cut(s) 191, 461, 1055, 1115
PaeI GCATGC 1 cut(s) 191
PaeR7I CTCGAG 1 cut(s) 610
PciSI GCTCTTC 1 cut(s) 235
PctI GAATGC 1 cut(s) 1204
PfeI GAWTC 7 cut(s) 125, 742, 789, 1427, 1466, 1530, 1928
Pfl23II CGTACG 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 2091
PkrI GCNGC 2 cut(s) 1360, 1450
PleI GAGTC 4 cut(s) 61, 78, 93, 1887
PpsI GAGTC 4 cut(s) 61, 78, 93, 1887
PshBI ATTAAT 1 cut(s) 923
PsiI TTATAA 1 cut(s) 1764
Psp124BI GAGCTC 1 cut(s) 1375
Psp6I CCWGG 2 cut(s) 1944, 2091
PspEI GGTNACC 1 cut(s) 195
PspGI CCWGG 2 cut(s) 1944, 2091
PspLI CGTACG 1 cut(s) 587
PspN4I GGNNCC 2 cut(s) 1386, 2097
PspPI GGNCC 4 cut(s) 46, 715, 1544, 2059
PspXI VCTCGAGB 1 cut(s) 610
PstI CTGCAG 2 cut(s) 1082, 1363
PsuI RGATCY 4 cut(s) 659, 875, 2095, 2134
RsaI GTAC 6 cut(s) 42, 589, 808, 1729, 1924, 1937
RsaNI GTAC 6 cut(s) 41, 588, 807, 1728, 1923, 1936
RseI CAYNNNNRTG 4 cut(s) 462, 681, 897, 1116
SacI GAGCTC 1 cut(s) 1375
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 4 cut(s) 236, 923, 1277, 1701
SatI GCNGC 2 cut(s) 1359, 1449
Sau96I GGNCC 4 cut(s) 46, 715, 1544, 2059
ScaI AGTACT 1 cut(s) 808
SchI GAGTC 4 cut(s) 62, 79, 94, 1887
ScrFI CCNGG 3 cut(s) 223, 1946, 2093
SduI GDGCHC 2 cut(s) 1375, 1389
SfaNI GCATC 2 cut(s) 1924, 2030
SfcI CTRYAG 4 cut(s) 1078, 1359, 1684, 2294
Sfr274I CTCGAG 1 cut(s) 610
SinI GGWCC 1 cut(s) 715
SlaI CTCGAG 1 cut(s) 610
SmiMI CAYNNNNRTG 4 cut(s) 462, 681, 897, 1116
SmlI CTYRAG 3 cut(s) 173, 263, 610
SmoI CTYRAG 3 cut(s) 173, 263, 610
SpeI ACTAGT 1 cut(s) 809
SphI GCATGC 1 cut(s) 191
SsiI CCGC 2 cut(s) 643, 2174
SspI AATATT 1 cut(s) 161
SspMI CTAG 5 cut(s) 78, 516, 810, 1410, 1757
SstI GAGCTC 1 cut(s) 1375
StyD4I CCNGG 3 cut(s) 221, 1944, 2091
TaaI ACNGT 5 cut(s) 234, 587, 719, 806, 1259
TaqI TCGA 8 cut(s) 94, 611, 853, 1072, 1565, 1662, 1803, 1918
TaqII GACCGA 1 cut(s) 1260
TatI WGTACW 2 cut(s) 806, 1922
TfiI GAWTC 7 cut(s) 125, 742, 789, 1427, 1466, 1530, 1928
Tru1I TTAA 4 cut(s) 236, 923, 1277, 1701
Tru9I TTAA 4 cut(s) 236, 923, 1277, 1701
TscAI CASTG 2 cut(s) 940, 1082
TseFI GTSAC 4 cut(s) 116, 195, 500, 2120
TseI GCWGC 2 cut(s) 1358, 1448
Tsp45I GTSAC 4 cut(s) 116, 195, 500, 2120
TspDTI ATGAA 8 cut(s) 789, 1154, 1256, 1384, 1479, 1599, 1973, 2223
TspGWI ACGGA 3 cut(s) 63, 362, 1296
TspRI CASTG 2 cut(s) 940, 1082
VpaK11BI GGWCC 1 cut(s) 715
VspI ATTAAT 1 cut(s) 923
XagI CCTNNNNNAGG 1 cut(s) 2079
XapI RAATTY 5 cut(s) 307, 352, 1218, 1627, 2254
XceI RCATGY 4 cut(s) 191, 461, 1055, 1115
XhoI CTCGAG 1 cut(s) 610
XmiI GTMKAC 4 cut(s) 436, 652, 1087, 1683
XspI CTAG 5 cut(s) 78, 516, 810, 1410, 1757
ZrmI AGTACT 1 cut(s) 808
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.