Rorug03G0111100

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000875
Physical Location & Seq
Forward (+)
8984147 .. 8986274
2128 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0111100.1

Sequence Viewer

Length: 1047 bp
ATGGAGGGGATGAGCGGCGAGTCTGAGTGCTCGAAGACAAGTCCCTCCGATGACCGAAATGAGGAAGGAAGTGAGAGTGGAGAGAACTACGATGGCGAAATCAGTAAGCCCAAAAACAATGGAGGAAGCTCAAGCAACAGCACGGTAGAAGAAAGTGATCAGAAAAAGGGATCGGTGCGACCCTATGTTAGATCCAAGATGCCAAGGCTCAGGTGGACACCGGATCTCCATCTTCGCTTTGTTCACGCCGTGGAGAGGCTCGGAGGACAAGATAGAGCTACACCAAAGATGGTTCTTCAGCTTATGAACATCAAGGGGCTAAGTATTGCACATGTCAAGAGCCATTTACAGATGTATAGAAGCAAGAAGATCGACGATGCAGGCCAAGTGATAGCAGATCAAGGACATCATCTTGCTGAATGTGGAGATAAAAATATATACAACCTCAGCCAACTTCCCATGCTTCAAGGATACAACCGAAGCCACATGAGTACCAGCTTCAGATATGCATACGGTGATGCCAATTCTTGGAGTAATAGTGCTTATGAGAACTTGAGGCATCCAAGAATATTTCAAGACACAATGACAGAGAAGCTCTTTGGTAGCAGTACTACAACTAGCAACTGGAATAGTACTGCTTACAGCAATTTCCGGACAAGCTCCGAACAAGTACCAACTTGGATAACTCATACACTCAAAGACGAATGCAGTCAATTGTATAACATTCGTCGACAGTCCTTGCAAGCACATCAAACTAGGCAGAGTCTCATTGATCATCTTAATCCCACAACCCATGTGCAACCCAAAGCAAAGGATCACTTTACAAGTTTGAGCAGCACACCAACCAATCTACAAGAGTTGAAGACGTTGAAAAGGAAGGCTTCGGATTGCGATCTTGATTTAGATCTGTCTCTCAGGCTAACAACGAAGAAGAATGACGAGAGTCCGAGAAGTACTACTATGAGGGACGATGAAGTTGATTGCACTAGTCTTTCTCTGTCATTGTACTCACCGGCATCGGCAAAAATCAGAAGGGTGGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

348

Amino Acids

39.33

Weight (kDa)

7.71

Isoelectric Point (pI)

59.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myb_DNA-binding PF00249 69 - 120 1.1e-07 Myb-like DNA-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 528
AccBSI CCGCTC 1 cut(s) 15
AccI GTMKAC 1 cut(s) 730
AccIII TCCGGA 1 cut(s) 651
AciI CCGC 1 cut(s) 15
AclWI GGATC 4 cut(s) 178, 186, 231, 822
AcuI CTGAAG 2 cut(s) 281, 484
AdeI CACNNNGTG 1 cut(s) 250
AfaI GTAC 6 cut(s) 493, 610, 634, 672, 955, 1007
AfiI CCNNNNNNNGG 3 cut(s) 61, 255, 528
AflIII ACRYGT 1 cut(s) 331
AgsI TTSAA 4 cut(s) 467, 575, 862, 871
AhlI ACTAGT 1 cut(s) 986
AluBI AGCT 6 cut(s) 129, 278, 301, 498, 595, 660
AluI AGCT 6 cut(s) 129, 278, 301, 498, 595, 660
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 770, 915
AlwI GGATC 4 cut(s) 178, 186, 231, 822
Aor13HI TCCGGA 1 cut(s) 651
AoxI GGCC 1 cut(s) 382
ApeKI GCWGC 1 cut(s) 834
AsuHPI GGTGA 2 cut(s) 527, 1002
BaeI ACNNNNGTAYC 2 cut(s) 475, 508
BbsI GAAGAC 2 cut(s) 41, 869
Bbv12I GWGCWC 1 cut(s) 32
BbvCI CCTCAGC 1 cut(s) 446
BbvI GCAGC 1 cut(s) 846
BccI CCATC 3 cut(s) 86, 237, 283
BceAI ACGGC 1 cut(s) 233
BcgI CGANNNNNNTGC 2 cut(s) 352, 386
BciVI GTATCC 1 cut(s) 464
BclI TGATCA 2 cut(s) 157, 772
BcoDI GTCTC 2 cut(s) 770, 915
BcuI ACTAGT 1 cut(s) 986
BfaI CTAG 3 cut(s) 618, 756, 987
BfuI GTATCC 1 cut(s) 464
BglII AGATCT 1 cut(s) 904
BisI GCNGC 2 cut(s) 16, 835
BlsI GCNGC 2 cut(s) 17, 836
BmcAI AGTACT 3 cut(s) 610, 634, 955
BmsI GCATC 5 cut(s) 189, 367, 508, 568, 1025
BoxI GACNNNNGTC 1 cut(s) 942
BpiI GAAGAC 2 cut(s) 41, 869
Bpu10I CCTNAGC 2 cut(s) 209, 446
BpuEI CTTGAG 2 cut(s) 115, 574
BsaBI GATNNNNATC 3 cut(s) 228, 891, 903
BsaJI CCNNGG 2 cut(s) 203, 249
BsaWI WCCGGW 2 cut(s) 220, 651
BsaXI ACNNNNNCTCC 4 cut(s) 210, 240, 523, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 255, 528
Bse118I RCCGGY 1 cut(s) 1012
Bse1I ACTGG 1 cut(s) 629
Bse8I GATNNNNATC 3 cut(s) 228, 891, 903
BseAI TCCGGA 1 cut(s) 651
BseDI CCNNGG 2 cut(s) 203, 249
BseGI GGATG 2 cut(s) 15, 559
BseJI GATNNNNATC 3 cut(s) 228, 891, 903
BseLI CCNNNNNNNGG 3 cut(s) 61, 255, 528
BseMII CTCAG 4 cut(s) 15, 223, 460, 928
BseNI ACTGG 1 cut(s) 629
BseXI GCAGC 1 cut(s) 846
BshFI GGCC 1 cut(s) 384
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 3 cut(s) 221, 652, 1013
BslFI GGGAC 2 cut(s) 27, 980
BslI CCNNNNNNNGG 3 cut(s) 61, 255, 528
BsmAI GTCTC 2 cut(s) 770, 915
BsmFI GGGAC 2 cut(s) 27, 980
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 1 cut(s) 384
Bsp1286I GDGCHC 1 cut(s) 32
Bsp13I TCCGGA 1 cut(s) 651
BspACI CCGC 1 cut(s) 15
BspANI GGCC 1 cut(s) 384
BspCNI CTCAG 4 cut(s) 16, 222, 459, 927
BspEI TCCGGA 1 cut(s) 651
BspPI GGATC 4 cut(s) 178, 186, 231, 822
BsrBI CCGCTC 1 cut(s) 15
BsrFI RCCGGY 1 cut(s) 1012
BsrI ACTGG 1 cut(s) 629
BssAI RCCGGY 1 cut(s) 1012
BssECI CCNNGG 2 cut(s) 203, 249
BssT1I CCWWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 145, 515, 735
BstC8I GCNNGC 2 cut(s) 382, 744
BstDEI CTNAG 5 cut(s) 24, 209, 320, 446, 914
BstDSI CCRYGG 1 cut(s) 249
BstF5I GGATG 2 cut(s) 15, 559
BstMAI GTCTC 2 cut(s) 770, 915
BstNSI RCATGY 1 cut(s) 335
BstPAI GACNNNNGTC 1 cut(s) 942
BstV1I GCAGC 1 cut(s) 846
BstV2I GAAGAC 2 cut(s) 41, 869
BstX2I RGATCY 3 cut(s) 191, 223, 904
BstYI RGATCY 3 cut(s) 191, 223, 904
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 1 cut(s) 384
BtgI CCRYGG 1 cut(s) 249
BtsCI GGATG 2 cut(s) 15, 559
Cac8I GCNNGC 2 cut(s) 382, 744
Cfr10I RCCGGY 1 cut(s) 1012
Csp6I GTAC 6 cut(s) 492, 609, 633, 671, 954, 1006
CviAII CATG 4 cut(s) 332, 460, 487, 794
CviQI GTAC 6 cut(s) 492, 609, 633, 671, 954, 1006
DdeI CTNAG 5 cut(s) 24, 209, 320, 446, 914
DraIII CACNNNGTG 1 cut(s) 250
Eco130I CCWWGG 1 cut(s) 203
Eco57I CTGAAG 2 cut(s) 281, 484
EcoT14I CCWWGG 1 cut(s) 203
EcoT22I ATGCAT 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 203
FaeI CATG 4 cut(s) 335, 463, 490, 797
FalI AAGNNNNNCTT 4 cut(s) 803, 835, 865, 897
FaqI GGGAC 2 cut(s) 27, 980
FatI CATG 4 cut(s) 331, 459, 486, 793
FbaI TGATCA 2 cut(s) 157, 772
FblI GTMKAC 1 cut(s) 730
Fnu4HI GCNGC 2 cut(s) 16, 835
FokI GGATG 2 cut(s) 22, 546
Fsp4HI GCNGC 2 cut(s) 16, 835
FspBI CTAG 3 cut(s) 618, 756, 987
GluI GCNGC 2 cut(s) 16, 835
HaeIII GGCC 1 cut(s) 384
HapII CCGG 3 cut(s) 221, 652, 1013
Hin1II CATG 4 cut(s) 335, 463, 490, 797
HincII GTYRAC 1 cut(s) 731
HindII GTYRAC 1 cut(s) 731
HinfI GANTC 3 cut(s) 20, 763, 943
HpaII CCGG 3 cut(s) 221, 652, 1013
HphI GGTGA 2 cut(s) 527, 1002
Hpy166II GTNNAC 3 cut(s) 216, 244, 731
Hpy188I TCNGA 9 cut(s) 25, 49, 162, 263, 503, 664, 886, 948, 1031
Hpy188III TCNNGA 4 cut(s) 337, 575, 652, 896
Hpy8I GTNNAC 3 cut(s) 216, 244, 731
Hpy99I CGWCG 2 cut(s) 377, 732
HpyAV CCTTC 3 cut(s) 59, 871, 1026
HpyCH4III ACNGT 3 cut(s) 145, 515, 735
HpyCH4IV ACGT 1 cut(s) 866
HpyCH4V TGCA 7 cut(s) 329, 380, 509, 708, 742, 799, 984
HpyF3I CTNAG 5 cut(s) 24, 209, 320, 446, 914
HpySE526I ACGT 1 cut(s) 866
Hsp92II CATG 4 cut(s) 335, 463, 490, 797
Kpn2I TCCGGA 1 cut(s) 651
Ksp22I TGATCA 2 cut(s) 157, 772
LmnI GCTCC 1 cut(s) 665
LpnPI CCDG 8 cut(s) 196, 234, 366, 508, 610, 665, 901, 1026
Lsp1109I GCAGC 1 cut(s) 846
LweI GCATC 5 cut(s) 189, 367, 508, 568, 1025
MaeI CTAG 3 cut(s) 618, 756, 987
MaeII ACGT 1 cut(s) 866
MbiI CCGCTC 1 cut(s) 15
MboII GAAGA 8 cut(s) 46, 161, 224, 287, 379, 874, 940, 943
MfeI CAATTG 1 cut(s) 713
MflI RGATCY 3 cut(s) 191, 223, 904
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 3 cut(s) 523, 646, 713
MlyI GAGTC 3 cut(s) 29, 772, 952
MnlI CCTC 9 cut(s) 55, 55, 116, 249, 257, 455, 549, 957, 1033
Mph1103I ATGCAT 1 cut(s) 511
MroI TCCGGA 1 cut(s) 651
MseI TTAA 1 cut(s) 780
MspI CCGG 3 cut(s) 221, 652, 1013
MunI CAATTG 1 cut(s) 713
Mva1269I GAATGC 1 cut(s) 710
NlaIII CATG 4 cut(s) 335, 463, 490, 797
NsiI ATGCAT 1 cut(s) 511
NspI RCATGY 1 cut(s) 335
PciI ACATGT 1 cut(s) 331
PctI GAATGC 1 cut(s) 710
PflMI CCANNNNNTGG 1 cut(s) 528
PkrI GCNGC 2 cut(s) 17, 836
PleI GAGTC 3 cut(s) 28, 771, 951
PpsI GAGTC 3 cut(s) 28, 771, 951
PscI ACATGT 1 cut(s) 331
PshAI GACNNNNGTC 1 cut(s) 942
PsuI RGATCY 3 cut(s) 191, 223, 904
RsaI GTAC 6 cut(s) 493, 610, 634, 672, 955, 1007
RsaNI GTAC 6 cut(s) 492, 609, 633, 671, 954, 1006
SalI GTCGAC 1 cut(s) 729
SaqAI TTAA 1 cut(s) 780
SatI GCNGC 2 cut(s) 16, 835
ScaI AGTACT 3 cut(s) 610, 634, 955
SchI GAGTC 3 cut(s) 29, 772, 952
SduI GDGCHC 1 cut(s) 32
SetI ASST 9 cut(s) 131, 215, 280, 303, 447, 500, 597, 662, 869
SfaNI GCATC 5 cut(s) 189, 367, 508, 568, 1025
SmlI CTYRAG 2 cut(s) 130, 553
SmoI CTYRAG 2 cut(s) 130, 553
SpeI ACTAGT 1 cut(s) 986
Sse9I AATT 3 cut(s) 523, 646, 713
SsiI CCGC 1 cut(s) 15
SspI AATATT 1 cut(s) 570
SspMI CTAG 3 cut(s) 618, 756, 987
StyI CCWWGG 1 cut(s) 203
TaaI ACNGT 3 cut(s) 145, 515, 735
TaiI ACGT 1 cut(s) 869
TaqI TCGA 3 cut(s) 32, 372, 730
TaqII GACCGA 1 cut(s) 69
TasI AATT 3 cut(s) 523, 646, 713
TatI WGTACW 4 cut(s) 608, 632, 953, 1005
TauI GCSGC 1 cut(s) 18
Tru1I TTAA 1 cut(s) 780
Tru9I TTAA 1 cut(s) 780
TseI GCWGC 1 cut(s) 834
TspDTI ATGAA 2 cut(s) 320, 987
Van91I CCANNNNNTGG 1 cut(s) 528
XceI RCATGY 1 cut(s) 335
XcmI CCANNNNNNNNNTGG 1 cut(s) 210
XmiI GTMKAC 1 cut(s) 730
XspI CTAG 3 cut(s) 618, 756, 987
ZrmI AGTACT 3 cut(s) 610, 634, 955
Zsp2I ATGCAT 1 cut(s) 511
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.