MD14G1013900.v1.1

catalytic domain of ctd-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000875
Physical Location & Seq
Reverse (-)
1238639 .. 1240881
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1013900.v1.1.491

Sequence Viewer

Length: 801 bp
ATGGCTGATGAAAAGATTTGTAACAACAAGCTTCAATATGACAGCGATGAGGTCGACGATGATGACGGCGACGATGATTGCAACGAAGACACCGGAAGCGATTGCGGCCTTTTTCTGGAGAAGCTATACATAGGATCAAGGAAGAAGCTTGTAATCTTTAGCCTCAATGGCTTGCTAGTCCACAGAGTTATCCGCTACAACAGGGCAAAAATTCCGAGCAATCGTACTCCTGATGGCACATATGGAAGCTATCTAGTTTTTAGGAGACCATTTTGTGAGGAATTCATGCAATTTTGTTTTGAAAGATTCGAAGTTGGAATATTGAGTTCTGCCCAAGTAAAAAATGTGGATGGTGTTTTGGATTGTGTGATGGGAAAGCTGAGAAGGCGACTGTCATTTGTTTGGGATCAACATGAATGTACCGACTCTGGGTTCAAGTCGCTAGAGGATAATCGCAAGCCTCTATTTTTCAAAGAACTGGACAAAGTGTGGAGCTATTTTAGCGGGAAATACTCAGAATCTGATACACTTTTCATTGATGATCAACCTTACAAGGGTCTATTGAATCCTCCTAACACAGGTATCTTCTTGGAGTCATATGATGCTGAGAATGACAACGACAAAGAATTAGATCCAAAGCGAGAACTTGGGAAGTATTTGGATGGCCTTGCAGATGCAAAAGATGTGCAAATTTATGTGAAGGAAAACCCTTTTGGACAGCCTGCTGTTTCATCTACTCATCCTTATTGGAAATTCTACTCGAATGTTCTTCGTAGGCTCCGAAAGCAGTACGAGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

267

Amino Acids

30.97

Weight (kDa)

5.05

Isoelectric Point (pI)

31.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 49 - 230 7.2e-21 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 54
AciI CCGC 3 cut(s) 105, 193, 504
AclWI GGATC 3 cut(s) 142, 414, 626
AcsI RAATTY 4 cut(s) 210, 281, 690, 752
AfaI GTAC 3 cut(s) 226, 421, 791
AfiI CCNNNNNNNGG 4 cut(s) 115, 429, 554, 578
AgsI TTSAA 5 cut(s) 35, 302, 436, 472, 565
AjuI GAANNNNNNNTTGG 2 cut(s) 696, 728
AluBI AGCT 6 cut(s) 31, 124, 148, 249, 379, 495
AluI AGCT 6 cut(s) 31, 124, 148, 249, 379, 495
Alw26I GTCTC 1 cut(s) 259
AlwI GGATC 3 cut(s) 142, 414, 626
AlwNI CAGNNNCTG 1 cut(s) 521
AoxI GGCC 2 cut(s) 106, 664
ApoI RAATTY 4 cut(s) 210, 281, 690, 752
AsuII TTCGAA 1 cut(s) 309
BaeI ACNNNNGTAYC 2 cut(s) 565, 598
BbsI GAAGAC 1 cut(s) 93
BccI CCATC 4 cut(s) 227, 344, 364, 656
BceAI ACGGC 1 cut(s) 82
BclI TGATCA 1 cut(s) 541
BcoDI GTCTC 1 cut(s) 259
BfaI CTAG 3 cut(s) 176, 254, 443
BglI GCCNNNNNGGC 1 cut(s) 168
BisI GCNGC 1 cut(s) 106
BlsI GCNGC 1 cut(s) 107
BmiI GGNNCC 1 cut(s) 779
BmsI GCATC 2 cut(s) 592, 664
BpiI GAAGAC 1 cut(s) 93
BpmI CTGGAG 1 cut(s) 137
Bpu14I TTCGAA 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 259
BsaWI WCCGGW 1 cut(s) 92
Bsc4I CCNNNNNNNGG 4 cut(s) 115, 429, 554, 578
Bse1I ACTGG 1 cut(s) 483
BseGI GGATG 3 cut(s) 355, 667, 739
BseLI CCNNNNNNNGG 4 cut(s) 115, 429, 554, 578
BseMII CTCAG 3 cut(s) 371, 528, 597
BseNI ACTGG 1 cut(s) 483
BshFI GGCC 2 cut(s) 108, 666
BsiSI CCGG 1 cut(s) 93
BslI CCNNNNNNNGG 4 cut(s) 115, 429, 554, 578
BsmAI GTCTC 1 cut(s) 259
BsnI GGCC 2 cut(s) 108, 666
Bso31I GGTCTC 1 cut(s) 259
Bsp119I TTCGAA 1 cut(s) 309
Bsp143I GATC 4 cut(s) 134, 406, 541, 631
BspACI CCGC 3 cut(s) 105, 193, 504
BspANI GGCC 2 cut(s) 108, 666
BspCNI CTCAG 3 cut(s) 372, 527, 598
BspLI GGNNCC 1 cut(s) 779
BspPI GGATC 3 cut(s) 142, 414, 626
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 259
BsrI ACTGG 1 cut(s) 483
BssMI GATC 4 cut(s) 134, 406, 541, 631
Bst4CI ACNGT 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 3 cut(s) 173, 458, 723
BstDEI CTNAG 3 cut(s) 380, 514, 606
BstENI CCTNNNNNAGG 1 cut(s) 576
BstF5I GGATG 3 cut(s) 355, 667, 739
BstKTI GATC 4 cut(s) 137, 409, 544, 634
BstMAI GTCTC 1 cut(s) 259
BstMBI GATC 4 cut(s) 134, 406, 541, 631
BstMWI GCNNNNNNNGC 6 cut(s) 105, 168, 385, 501, 784, 793
BstV2I GAAGAC 1 cut(s) 93
BstX2I RGATCY 1 cut(s) 631
BstYI RGATCY 1 cut(s) 631
BsuRI GGCC 2 cut(s) 108, 666
BtgZI GCGATG 1 cut(s) 60
BtsCI GGATG 3 cut(s) 355, 667, 739
Cac8I GCNNGC 3 cut(s) 173, 458, 723
CaiI CAGNNNCTG 1 cut(s) 521
Csp6I GTAC 3 cut(s) 225, 420, 790
CviAII CATG 2 cut(s) 286, 413
CviQI GTAC 3 cut(s) 225, 420, 790
DdeI CTNAG 3 cut(s) 380, 514, 606
DpnI GATC 4 cut(s) 136, 408, 543, 633
DpnII GATC 4 cut(s) 134, 406, 541, 631
Eco31I GGTCTC 1 cut(s) 259
EcoNI CCTNNNNNAGG 1 cut(s) 576
EcoRI GAATTC 1 cut(s) 281
FaeI CATG 2 cut(s) 289, 416
FatI CATG 2 cut(s) 285, 412
FauI CCCGC 1 cut(s) 497
FauNDI CATATG 2 cut(s) 241, 598
FbaI TGATCA 1 cut(s) 541
FblI GTMKAC 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 106
FokI GGATG 3 cut(s) 362, 674, 726
Fsp4HI GCNGC 1 cut(s) 106
FspBI CTAG 3 cut(s) 176, 254, 443
GluI GCNGC 1 cut(s) 106
GsuI CTGGAG 1 cut(s) 137
HaeIII GGCC 2 cut(s) 108, 666
HapII CCGG 1 cut(s) 93
Hin1II CATG 2 cut(s) 289, 416
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HindIII AAGCTT 2 cut(s) 29, 146
HinfI GANTC 5 cut(s) 306, 425, 518, 565, 593
HpaII CCGG 1 cut(s) 93
Hpy166II GTNNAC 2 cut(s) 55, 181
Hpy188I TCNGA 4 cut(s) 216, 517, 523, 782
Hpy188III TCNNGA 2 cut(s) 116, 230
Hpy8I GTNNAC 2 cut(s) 55, 181
Hpy99I CGWCG 2 cut(s) 59, 74
HpyAV CCTTC 2 cut(s) 378, 694
HpyCH4III ACNGT 1 cut(s) 393
HpyCH4V TGCA 5 cut(s) 81, 289, 671, 677, 688
HpyF10VI GCNNNNNNNGC 6 cut(s) 105, 168, 385, 501, 784, 793
HpyF3I CTNAG 3 cut(s) 380, 514, 606
Hsp92II CATG 2 cut(s) 289, 416
Ksp22I TGATCA 1 cut(s) 541
Kzo9I GATC 4 cut(s) 134, 406, 541, 631
LmnI GCTCC 2 cut(s) 492, 783
LpnPI CCDG 8 cut(s) 101, 106, 187, 243, 414, 464, 564, 735
LweI GCATC 2 cut(s) 592, 664
MaeI CTAG 3 cut(s) 176, 254, 443
MaeIII GTNAC 1 cut(s) 20
MalI GATC 4 cut(s) 136, 408, 543, 633
MboI GATC 4 cut(s) 134, 406, 541, 631
MboII GAAGA 4 cut(s) 98, 154, 577, 761
MflI RGATCY 1 cut(s) 631
MluCI AATT 6 cut(s) 210, 281, 290, 626, 690, 752
MlyI GAGTC 2 cut(s) 419, 602
MmeI TCCRAC 1 cut(s) 295
MnlI CCTC 6 cut(s) 43, 173, 271, 439, 471, 579
MspI CCGG 1 cut(s) 93
MwoI GCNNNNNNNGC 6 cut(s) 105, 168, 385, 501, 784, 793
NdeI CATATG 2 cut(s) 241, 598
NdeII GATC 4 cut(s) 134, 406, 541, 631
NlaIII CATG 2 cut(s) 289, 416
NlaIV GGNNCC 1 cut(s) 779
NspV TTCGAA 1 cut(s) 309
PcsI WCGNNNNNNNCGW 1 cut(s) 778
PfeI GAWTC 3 cut(s) 306, 518, 565
PkrI GCNGC 1 cut(s) 107
PleI GAGTC 2 cut(s) 419, 601
PpsI GAGTC 2 cut(s) 419, 601
PspN4I GGNNCC 1 cut(s) 779
PstNI CAGNNNCTG 1 cut(s) 521
PsuI RGATCY 1 cut(s) 631
RsaI GTAC 3 cut(s) 226, 421, 791
RsaNI GTAC 3 cut(s) 225, 420, 790
SalI GTCGAC 1 cut(s) 53
SatI GCNGC 1 cut(s) 106
Sau3AI GATC 4 cut(s) 134, 406, 541, 631
SchI GAGTC 2 cut(s) 419, 602
SetI ASST 9 cut(s) 33, 54, 126, 150, 251, 381, 497, 550, 583
SfaNI GCATC 2 cut(s) 592, 664
SfuI TTCGAA 1 cut(s) 309
Sse9I AATT 6 cut(s) 210, 281, 290, 626, 690, 752
SsiI CCGC 3 cut(s) 105, 193, 504
SspI AATATT 1 cut(s) 321
SspMI CTAG 3 cut(s) 176, 254, 443
TaaI ACNGT 1 cut(s) 393
TaqI TCGA 3 cut(s) 54, 309, 761
TasI AATT 6 cut(s) 210, 281, 290, 626, 690, 752
TauI GCSGC 1 cut(s) 108
TfiI GAWTC 3 cut(s) 306, 518, 565
TspDTI ATGAA 5 cut(s) 24, 274, 429, 523, 720
XagI CCTNNNNNAGG 1 cut(s) 576
XapI RAATTY 4 cut(s) 210, 281, 690, 752
XmiI GTMKAC 1 cut(s) 54
XspI CTAG 3 cut(s) 176, 254, 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.