Rh3AG176100

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0000875
Physical Location & Seq
Forward (+)
16419260 .. 16425153
5894 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG176100.1

Sequence Viewer

Length: 1908 bp
ATGAGGACACCATCCAGTTCTGCCATGTATATGCATGAGAAGTCCACTCGTGCATTTGCAAATTCCATGATGGCGAGAAATTTAACACATTATTCCGAACCTTCAGTACAGGACCATGGGTCTCCGCGTAGTAGGAATCCCTCTAGGAAGAGTCAATCTCGAACAGAAAGATCACCTACTCGTCATAGAAAGTCCCACAGAGGCAGTTCTCCACCAAGTGAGAAATATTCAATTCGCCCCAAGTCTCCGAAGCATGCAAGGTCACCCTCAGCTCGCTCTCCTTTTCTCCAGACAAAAAGGTTAAGAAGAGCTGAAGCTGGAGGAGTGCCTGAGAGAGAACAGGAAAGGATCAATGGTAGGGGGATCGGTCGGAGTTTACAGGAAGGTGGTTCTGAGGGAGATGTTGGAAGTGATAGAACGGAGAAATTTTTGGGAAGGAATGATACTGATGGTAATACTTCAAGATCAAGGCATGGTACTTCTCCATCAGATAGAAACCCCAGGAGTAGACATAGACACTCACCTCAACATGCTGGTGATACAAGAGATGAGGAGAGAGTAACTGAGGGCCGTAACAGAAATCATAGCAGAGGGAATGATAGAAGTATGCATAGGGAAAATGAGACTGTAAGAACGGAGAGAATGACAGCAAAAGACAATACTGGTCATATGTCTGCAAGATCAAGGCATGATACTTCTCCATCAGATCAACACCGCAGGAGTAGACATAGATATTACTCACCTCAACATGCTTGTGATACAAGACATGGGGAGAAAGTAATCGAGGGCCGTGAAAGAAATCATAGCAGAGGGAATCATAGAAGTATGCATATGGAAAATGAGACTGAAAGAATGGAGAGAATGACAGCAAGAGAGAGTACTAGTCATCGGTCTTCAAGATCAAGGCATAATACTTCTCCATCAGATCGACACCGCAGTAGACATAGATCTCACTCACCTCAACATACTGGTGATACAAGAGATAAGGAGAAAGTAATTGAGGGCCGTGAAAGAAATCATAGCCGAGGGAATTATAGAAGCATGCGTTGGGAAAATGACACTGAAAGAATGGAGAGAATGACAGCAAAAGAGAGTTCTGGTCATAGGTCTTCAAGATCAAGGCATGGTTCTTCTCCATCAGATCGACACCGCAGCAGTAGACATAGATATCACTCACCTCACCATGCTGGTGATACAAGAGATAAGGAGAAAGTAATTGAAGACTGTCAAAGAAATTATAGGAGAGAGAACGATAGAATTATGCATAGGGAAAAGCATTCAGATAGGAAATATGAGACTGAAAGAATGGAGACAAGGACAGCAAAAGACAGTTCTGGTCATAGGTCTGCAAGAAGACACACGCAATCTACTTCTCCATTGAATGGACACCACAAGAACAGACACCGAACTCATTCATCTCAACAGGTTGCAGATATCAGAGCTCGCAATGAGGCGCCTCAGTCGAGGGAGGCTAAGGATGGGATTGAAAAGAATGATTCAGTAGCTATGATGAGCGCTGCTGAGGAAGTCTTGGAAGCACAGAAAAAGCAAAATCCTTCATTTGAGTTATCTGGAAAGTTTGCTATGGAAACCAATAGATTCAGAGGAGATAGCTATAAGAAGAATCTTCTTTCGAGTAGTAACTATGATGATGATGAAGACTATGATAATGAAAGAACACGAGGCATATGTAGGCTGAATCTAGCACCAAGAAAAAAAAAGCTTCTTGTGTTTGATCTCAATGGCTTACTAGTCTACAGAGTTTTTTGTTTCAACAAGAGTGGTATTCCAAGGGATCGTACTCCTGATGGGAAATGTGGAAACCATCTAGGTAGTAATGCAAACTATTATTTGTTCAATCTAACCATTTATGAAATTGTGGATCACAATTTATTTGGACATATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

635

Amino Acids

73.83

Weight (kDa)

10.48

Isoelectric Point (pI)

76.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1453
AccB7I CCANNNNNTGG 1 cut(s) 1382
AccI GTMKAC 5 cut(s) 508, 724, 940, 1159, 1755
AccII CGCG 1 cut(s) 127
AciI CCGC 4 cut(s) 125, 715, 934, 1150
AclWI GGATC 4 cut(s) 356, 371, 1803, 1890
AcsI RAATTY 3 cut(s) 61, 79, 425
AcuI CTGAAG 2 cut(s) 87, 333
AcyI GRCGYC 1 cut(s) 1454
AdeI CACNNNGTG 1 cut(s) 218
AfaI GTAC 4 cut(s) 108, 478, 880, 1801
AfeI AGCGCT 1 cut(s) 1516
AfiI CCNNNNNNNGG 1 cut(s) 1382
AgsI TTSAA 9 cut(s) 231, 462, 897, 1113, 1220, 1381, 1487, 1774, 1858
AhdI GACNNNNNGTC 1 cut(s) 118
AhlI ACTAGT 2 cut(s) 881, 1750
AjnI CCWGG 1 cut(s) 500
AjuI GAANNNNNNNTTGG 2 cut(s) 1030, 1062
AluBI AGCT 7 cut(s) 272, 311, 317, 1442, 1505, 1614, 1723
AluI AGCT 7 cut(s) 272, 311, 317, 1442, 1505, 1614, 1723
Alw21I GWGCWC 1 cut(s) 1444
Alw26I GTCTC 6 cut(s) 126, 249, 617, 836, 1289, 1304
AlwI GGATC 4 cut(s) 356, 371, 1803, 1890
Aor51HI AGCGCT 1 cut(s) 1516
AoxI GGCC 3 cut(s) 568, 787, 1003
ApeKI GCWGC 2 cut(s) 1152, 1517
ApoI RAATTY 3 cut(s) 61, 79, 425
Asp700I GAANNNNTTC 1 cut(s) 1411
AspLEI GCGC 2 cut(s) 1456, 1517
AspS9I GGNCC 4 cut(s) 112, 568, 787, 1003
AvaII GGWCC 1 cut(s) 112
BanI GGYRCC 1 cut(s) 1453
BanII GRGCYC 1 cut(s) 1444
BarI GAAGNNNNNNTAC 2 cut(s) 427, 459
BauI CACGAG 2 cut(s) 48, 1680
BbsI GAAGAC 5 cut(s) 885, 1101, 1227, 1360, 1665
Bbv12I GWGCWC 1 cut(s) 1444
BbvCI CCTCAGC 2 cut(s) 268, 1521
BbvI GCAGC 2 cut(s) 1164, 1504
BceAI ACGGC 3 cut(s) 555, 774, 990
BciT130I CCWGG 1 cut(s) 502
BcoDI GTCTC 6 cut(s) 126, 249, 617, 836, 1289, 1304
BcuI ACTAGT 2 cut(s) 881, 1750
BfaI CTAG 5 cut(s) 144, 882, 1703, 1751, 1829
BfmI CTRYAG 1 cut(s) 1756
BfoI RGCGCY 2 cut(s) 1457, 1518
BglII AGATCT 1 cut(s) 947
BisI GCNGC 2 cut(s) 1153, 1518
BlsI GCNGC 2 cut(s) 1154, 1519
BmcAI AGTACT 1 cut(s) 880
Bme1390I CCNGG 1 cut(s) 502
Bme18I GGWCC 1 cut(s) 112
BmeRI GACNNNNNGTC 1 cut(s) 118
BmgT120I GGNCC 4 cut(s) 112, 568, 787, 1003
BmiI GGNNCC 1 cut(s) 1455
BmrFI CCNGG 1 cut(s) 502
BpiI GAAGAC 5 cut(s) 885, 1101, 1227, 1360, 1665
BplI GAGNNNNNCTC 2 cut(s) 142, 174
BpmI CTGGAG 2 cut(s) 272, 339
Bpu10I CCTNAGC 3 cut(s) 268, 1473, 1521
BsaHI GRCGYC 1 cut(s) 1454
BsaI GGTCTC 1 cut(s) 126
BsaJI CCNNGG 4 cut(s) 115, 500, 1024, 1790
Bsc4I CCNNNNNNNGG 1 cut(s) 1382
Bse1I ACTGG 3 cut(s) 15, 667, 973
Bse3DI GCAATG 1 cut(s) 1453
BseBI CCWGG 1 cut(s) 502
BseDI CCNNGG 4 cut(s) 115, 500, 1024, 1790
BseGI GGATG 2 cut(s) 11, 1483
BseLI CCNNNNNNNGG 1 cut(s) 1382
BseMI GCAATG 1 cut(s) 1453
BseMII CTCAG 6 cut(s) 282, 321, 384, 555, 1472, 1512
BseNI ACTGG 3 cut(s) 15, 667, 973
BseRI GAGGAG 3 cut(s) 336, 566, 1620
BseXI GCAGC 2 cut(s) 1164, 1504
Bsh1236I CGCG 1 cut(s) 127
Bsh1285I CGRYCG 1 cut(s) 370
BshFI GGCC 3 cut(s) 570, 789, 1005
BshNI GGYRCC 1 cut(s) 1453
BsiEI CGRYCG 1 cut(s) 370
BsiHKAI GWGCWC 1 cut(s) 1444
BslFI GGGAC 1 cut(s) 178
BslI CCNNNNNNNGG 1 cut(s) 1382
BsmAI GTCTC 6 cut(s) 126, 249, 617, 836, 1289, 1304
BsmFI GGGAC 1 cut(s) 178
BsmI GAATGC 1 cut(s) 1276
BsnI GGCC 3 cut(s) 570, 789, 1005
Bso31I GGTCTC 1 cut(s) 126
Bsp1286I GDGCHC 1 cut(s) 1444
Bsp19I CCATGG 1 cut(s) 115
BspACI CCGC 4 cut(s) 125, 715, 934, 1150
BspANI GGCC 3 cut(s) 570, 789, 1005
BspCNI CTCAG 6 cut(s) 281, 322, 385, 556, 1471, 1513
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 1 cut(s) 1455
BspPI GGATC 4 cut(s) 356, 371, 1803, 1890
BspQI GCTCTTC 1 cut(s) 301
BspT107I GGYRCC 1 cut(s) 1453
BspTNI GGTCTC 1 cut(s) 126
BsrDI GCAATG 1 cut(s) 1453
BsrI ACTGG 3 cut(s) 15, 667, 973
BssECI CCNNGG 4 cut(s) 115, 500, 1024, 1790
BssNI GRCGYC 1 cut(s) 1454
BssSI CACGAG 2 cut(s) 48, 1680
BssT1I CCWWGG 2 cut(s) 115, 1790
Bst2BI CACGAG 2 cut(s) 48, 1680
Bst2UI CCWGG 1 cut(s) 502
Bst4CI ACNGT 3 cut(s) 628, 1226, 1331
Bst6I CTCTTC 2 cut(s) 143, 301
BstACI GRCGYC 1 cut(s) 1454
BstC8I GCNNGC 4 cut(s) 255, 274, 1043, 1444
BstDEI CTNAG 7 cut(s) 268, 330, 393, 564, 1458, 1473, 1521
BstDSI CCRYGG 1 cut(s) 115
BstEII GGTNACC 1 cut(s) 261
BstF5I GGATG 2 cut(s) 11, 1483
BstFNI CGCG 1 cut(s) 127
BstH2I RGCGCY 2 cut(s) 1457, 1518
BstHHI GCGC 2 cut(s) 1456, 1517
BstMAI GTCTC 6 cut(s) 126, 249, 617, 836, 1289, 1304
BstMCI CGRYCG 1 cut(s) 370
BstNI CCWGG 1 cut(s) 502
BstNSI RCATGY 4 cut(s) 257, 533, 752, 1045
BstPI GGTNACC 1 cut(s) 261
BstSCI CCNGG 1 cut(s) 500
BstSFI CTRYAG 1 cut(s) 1756
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 2 cut(s) 1164, 1504
BstV2I GAAGAC 5 cut(s) 885, 1101, 1227, 1360, 1665
BstX2I RGATCY 1 cut(s) 947
BstYI RGATCY 1 cut(s) 947
BsuRI GGCC 3 cut(s) 570, 789, 1005
BtgI CCRYGG 1 cut(s) 115
BtsCI GGATG 2 cut(s) 11, 1483
BtsIMutI CAGTG 1 cut(s) 1059
Cac8I GCNNGC 4 cut(s) 255, 274, 1043, 1444
CfoI GCGC 2 cut(s) 1456, 1517
Cfr13I GGNCC 4 cut(s) 112, 568, 787, 1003
Csp6I GTAC 4 cut(s) 107, 477, 879, 1800
CspCI CAANNNNNGTGG 2 cut(s) 1762, 1797
CviQI GTAC 4 cut(s) 107, 477, 879, 1800
DdeI CTNAG 7 cut(s) 268, 330, 393, 564, 1458, 1473, 1521
DinI GGCGCC 1 cut(s) 1455
DraIII CACNNNGTG 1 cut(s) 218
DriI GACNNNNNGTC 1 cut(s) 118
Eam1104I CTCTTC 2 cut(s) 143, 301
Eam1105I GACNNNNNGTC 1 cut(s) 118
EarI CTCTTC 2 cut(s) 143, 301
Ecl136II GAGCTC 1 cut(s) 1442
Eco130I CCWWGG 2 cut(s) 115, 1790
Eco24I GRGCYC 1 cut(s) 1444
Eco31I GGTCTC 1 cut(s) 126
Eco32I GATATC 2 cut(s) 1169, 1435
Eco47I GGWCC 1 cut(s) 112
Eco47III AGCGCT 1 cut(s) 1516
Eco53kI GAGCTC 1 cut(s) 1442
Eco57I CTGAAG 2 cut(s) 87, 333
Eco91I GGTNACC 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 1442
EcoO65I GGTNACC 1 cut(s) 261
EcoRII CCWGG 1 cut(s) 500
EcoRV GATATC 2 cut(s) 1169, 1435
EcoT14I CCWWGG 2 cut(s) 115, 1790
EcoT22I ATGCAT 4 cut(s) 36, 612, 831, 1266
EcoT38I GRGCYC 1 cut(s) 1444
EgeI GGCGCC 1 cut(s) 1455
EheI GGCGCC 1 cut(s) 1455
ErhI CCWWGG 2 cut(s) 115, 1790
FaqI GGGAC 1 cut(s) 178
FauNDI CATATG 3 cut(s) 669, 831, 1688
FblI GTMKAC 5 cut(s) 508, 724, 940, 1159, 1755
Fnu4HI GCNGC 2 cut(s) 1153, 1518
FokI GGATG 1 cut(s) 1490
FriOI GRGCYC 1 cut(s) 1444
Fsp4HI GCNGC 2 cut(s) 1153, 1518
FspBI CTAG 5 cut(s) 144, 882, 1703, 1751, 1829
GlaI GCGC 2 cut(s) 1455, 1516
GluI GCNGC 2 cut(s) 1153, 1518
GsuI CTGGAG 2 cut(s) 272, 339
HaeII RGCGCY 2 cut(s) 1457, 1518
HaeIII GGCC 3 cut(s) 570, 789, 1005
HhaI GCGC 2 cut(s) 1456, 1517
Hin1I GRCGYC 1 cut(s) 1454
Hin6I GCGC 2 cut(s) 1454, 1515
HinP1I GCGC 2 cut(s) 1454, 1515
HindIII AAGCTT 1 cut(s) 1721
HinfI GANTC 7 cut(s) 136, 151, 814, 1496, 1599, 1624, 1699
Hpy166II GTNNAC 7 cut(s) 45, 377, 509, 725, 941, 1160, 1756
Hpy188III TCNNGA 7 cut(s) 159, 289, 462, 897, 1113, 1572, 1805
Hpy8I GTNNAC 7 cut(s) 45, 377, 509, 725, 941, 1160, 1756
HpyAV CCTTC 4 cut(s) 111, 377, 429, 1566
HpyCH4III ACNGT 3 cut(s) 628, 1226, 1331
HpyF3I CTNAG 7 cut(s) 268, 330, 393, 564, 1458, 1473, 1521
Hsp92I GRCGYC 1 cut(s) 1454
HspAI GCGC 2 cut(s) 1454, 1515
KasI GGCGCC 1 cut(s) 1453
LguI GCTCTTC 1 cut(s) 301
Lsp1109I GCAGC 2 cut(s) 1164, 1504
MaeI CTAG 5 cut(s) 144, 882, 1703, 1751, 1829
MaeIII GTNAC 4 cut(s) 261, 559, 572, 1640
MflI RGATCY 1 cut(s) 947
MhlI GDGCHC 1 cut(s) 1444
Mly113I GGCGCC 1 cut(s) 1454
MlyI GAGTC 1 cut(s) 160
MmeI TCCRAC 2 cut(s) 350, 385
Mph1103I ATGCAT 4 cut(s) 36, 612, 831, 1266
MroXI GAANNNNTTC 1 cut(s) 1411
MseI TTAA 2 cut(s) 83, 302
MslI CAYNNNNRTG 5 cut(s) 29, 534, 753, 969, 1188
MspR9I CCNGG 1 cut(s) 502
Mva1269I GAATGC 1 cut(s) 1276
MvaI CCWGG 1 cut(s) 502
MvnI CGCG 1 cut(s) 127
NarI GGCGCC 1 cut(s) 1454
NcoI CCATGG 1 cut(s) 115
NdeI CATATG 3 cut(s) 669, 831, 1688
NlaIV GGNNCC 1 cut(s) 1455
NmeAIII GCCGAG 1 cut(s) 1049
NmuCI GTSAC 1 cut(s) 261
NsiI ATGCAT 4 cut(s) 36, 612, 831, 1266
NspI RCATGY 4 cut(s) 257, 533, 752, 1045
PaeI GCATGC 2 cut(s) 257, 1045
PciSI GCTCTTC 1 cut(s) 301
PctI GAATGC 1 cut(s) 1276
PdmI GAANNNNTTC 1 cut(s) 1411
PfeI GAWTC 6 cut(s) 136, 814, 1496, 1599, 1624, 1699
PflMI CCANNNNNTGG 1 cut(s) 1382
PkrI GCNGC 2 cut(s) 1154, 1519
PleI GAGTC 1 cut(s) 159
PluTI GGCGCC 1 cut(s) 1457
PpsI GAGTC 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 1444
Psp6I CCWGG 1 cut(s) 500
PspEI GGTNACC 1 cut(s) 261
PspGI CCWGG 1 cut(s) 500
PspN4I GGNNCC 1 cut(s) 1455
PspPI GGNCC 4 cut(s) 112, 568, 787, 1003
PsrI GAACNNNNNNTAC 2 cut(s) 90, 122
PsuI RGATCY 1 cut(s) 947
RsaI GTAC 4 cut(s) 108, 478, 880, 1801
RsaNI GTAC 4 cut(s) 107, 477, 879, 1800
RseI CAYNNNNRTG 5 cut(s) 29, 534, 753, 969, 1188
SacI GAGCTC 1 cut(s) 1444
SapI GCTCTTC 1 cut(s) 301
SaqAI TTAA 2 cut(s) 83, 302
SatI GCNGC 2 cut(s) 1153, 1518
Sau96I GGNCC 4 cut(s) 112, 568, 787, 1003
ScaI AGTACT 1 cut(s) 880
SchI GAGTC 1 cut(s) 160
ScrFI CCNGG 1 cut(s) 502
SduI GDGCHC 1 cut(s) 1444
SfcI CTRYAG 1 cut(s) 1756
SfoI GGCGCC 1 cut(s) 1455
SinI GGWCC 1 cut(s) 112
SmiMI CAYNNNNRTG 5 cut(s) 29, 534, 753, 969, 1188
SpeI ACTAGT 2 cut(s) 881, 1750
SphI GCATGC 2 cut(s) 257, 1045
SsiI CCGC 4 cut(s) 125, 715, 934, 1150
SspDI GGCGCC 1 cut(s) 1453
SspI AATATT 1 cut(s) 227
SspMI CTAG 5 cut(s) 144, 882, 1703, 1751, 1829
SstI GAGCTC 1 cut(s) 1444
StyD4I CCNGG 1 cut(s) 500
StyI CCWWGG 2 cut(s) 115, 1790
TaaI ACNGT 3 cut(s) 628, 1226, 1331
TaqI TCGA 6 cut(s) 160, 783, 928, 1144, 1463, 1634
TaqII GACCGA 2 cut(s) 356, 879
TatI WGTACW 2 cut(s) 106, 878
TfiI GAWTC 6 cut(s) 136, 814, 1496, 1599, 1624, 1699
Tru1I TTAA 2 cut(s) 83, 302
Tru9I TTAA 2 cut(s) 83, 302
TscAI CASTG 1 cut(s) 1066
TseFI GTSAC 1 cut(s) 261
TseI GCWGC 2 cut(s) 1152, 1517
Tsp45I GTSAC 1 cut(s) 261
TspDTI ATGAA 5 cut(s) 1404, 1548, 1671, 1686, 1887
TspGWI ACGGA 2 cut(s) 434, 650
TspRI CASTG 1 cut(s) 1066
Van91I CCANNNNNTGG 1 cut(s) 1382
VpaK11BI GGWCC 1 cut(s) 112
XapI RAATTY 3 cut(s) 61, 79, 425
XceI RCATGY 4 cut(s) 257, 533, 752, 1045
XmiI GTMKAC 5 cut(s) 508, 724, 940, 1159, 1755
XmnI GAANNNNTTC 1 cut(s) 1411
XspI CTAG 5 cut(s) 144, 882, 1703, 1751, 1829
ZrmI AGTACT 1 cut(s) 880
Zsp2I ATGCAT 4 cut(s) 36, 612, 831, 1266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.