Rroxscaffold_6G00410650

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000875
Physical Location & Seq
Reverse (-)
33058885 .. 33061183
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00410650.1

Sequence Viewer

Length: 537 bp
ATGAAATTTTGTCTGGAAAGATTTGAAGTGGGAATATGGTCTTCTGCAAGGGAATGGTACTTGGACAATGCCTTGGATTGTGTGATGAAAGGGTTGGGGAGGAAGTTGTTGTTTGCTTGGGATCAAGTGGAGTGTACTGATTCAGGGTTCAAAACCTTGGAGAACCAAAACAAGCCCCTCTTTCTGAAAGAGTTGAAGAAAATATGGGAAAACAAGGGTTTCAAAGCCTCACTTCCATCTTCCATGGGGAAATACTCTTCATTGAACACCTTGTTAATTGATGATAAGGCTTACAAGGCTCTACTAAATCCTGCTCACACAGCCATCTTTCCTCCTGAATACAAGGTTGACCAAGTTGGTGACAAGGCTTTAGGTCCTAATGGTGAGTTGAGGCTTTACTTGGAGGGACTTGCACAGCTGCCGATGGATGTTACTTCTTATGTTAAAAATCATCCATTTGGACAGCCAGCAATAACAACTACTCACTCTGATTGGGATTTCTATTCAAAGATCCTTACTCATTTCCAGAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

178

Amino Acids

20.36

Weight (kDa)

6.9

Isoelectric Point (pI)

19.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 2 - 144 7.4e-06 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 129, 505
AcsI RAATTY 1 cut(s) 5
AfaI GTAC 2 cut(s) 59, 136
AgsI TTSAA 6 cut(s) 26, 151, 196, 223, 265, 507
AluBI AGCT 1 cut(s) 418
AluI AGCT 1 cut(s) 418
AlwI GGATC 2 cut(s) 129, 505
ApeKI GCWGC 1 cut(s) 418
ApoI RAATTY 1 cut(s) 5
AspS9I GGNCC 1 cut(s) 374
AsuHPI GGTGA 2 cut(s) 371, 395
AvaII GGWCC 1 cut(s) 374
BbsI GAAGAC 1 cut(s) 33
BbvI GCAGC 1 cut(s) 405
BccI CCATC 3 cut(s) 244, 332, 418
BisI GCNGC 1 cut(s) 419
BlsI GCNGC 1 cut(s) 420
Bme18I GGWCC 1 cut(s) 374
BmgT120I GGNCC 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 72, 156, 243
BseDI CCNNGG 3 cut(s) 72, 156, 243
BseGI GGATG 2 cut(s) 433, 451
BseXI GCAGC 1 cut(s) 405
BslFI GGGAC 1 cut(s) 420
BsmFI GGGAC 1 cut(s) 420
Bsp143I GATC 2 cut(s) 121, 510
Bsp19I CCATGG 1 cut(s) 243
BspPI GGATC 2 cut(s) 129, 505
BssECI CCNNGG 3 cut(s) 72, 156, 243
BssMI GATC 2 cut(s) 121, 510
BssT1I CCWWGG 3 cut(s) 72, 156, 243
Bst6I CTCTTC 1 cut(s) 262
BstC8I GCNNGC 1 cut(s) 468
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 2 cut(s) 433, 451
BstKTI GATC 2 cut(s) 124, 513
BstMBI GATC 2 cut(s) 121, 510
BstMWI GCNNNNNNNGC 2 cut(s) 296, 320
BstV1I GCAGC 1 cut(s) 405
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 510
BstYI RGATCY 1 cut(s) 510
BtgI CCRYGG 1 cut(s) 243
BtsCI GGATG 2 cut(s) 433, 451
Cac8I GCNNGC 1 cut(s) 468
Cfr13I GGNCC 1 cut(s) 374
Csp6I GTAC 2 cut(s) 58, 135
CviAII CATG 1 cut(s) 244
CviJI RGCY 9 cut(s) 175, 227, 290, 299, 323, 368, 394, 418, 466
CviKI_1 RGCY 9 cut(s) 175, 227, 290, 299, 323, 368, 394, 418, 466
CviQI GTAC 2 cut(s) 58, 135
DpnI GATC 2 cut(s) 123, 512
DpnII GATC 2 cut(s) 121, 510
Eam1104I CTCTTC 1 cut(s) 262
EarI CTCTTC 1 cut(s) 262
Eco130I CCWWGG 3 cut(s) 72, 156, 243
Eco47I GGWCC 1 cut(s) 374
EcoO109I RGGNCCY 1 cut(s) 374
EcoT14I CCWWGG 3 cut(s) 72, 156, 243
ErhI CCWWGG 3 cut(s) 72, 156, 243
FaeI CATG 1 cut(s) 247
FaiI YATR 4 cut(s) 37, 205, 245, 441
FalI AAGNNNNNCTT 4 cut(s) 164, 196, 216, 248
FaqI GGGAC 1 cut(s) 420
FatI CATG 1 cut(s) 243
Fnu4HI GCNGC 1 cut(s) 419
FokI GGATG 2 cut(s) 438, 440
Fsp4HI GCNGC 1 cut(s) 419
GluI GCNGC 1 cut(s) 419
Hin1II CATG 1 cut(s) 247
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HinfI GANTC 1 cut(s) 140
HphI GGTGA 2 cut(s) 371, 395
Hpy166II GTNNAC 2 cut(s) 135, 349
Hpy188I TCNGA 2 cut(s) 186, 490
Hpy188III TCNNGA 3 cut(s) 14, 335, 526
Hpy8I GTNNAC 2 cut(s) 135, 349
HpyCH4V TGCA 2 cut(s) 47, 413
HpyF10VI GCNNNNNNNGC 2 cut(s) 296, 320
Hsp92II CATG 1 cut(s) 247
Kzo9I GATC 2 cut(s) 121, 510
LpnPI CCDG 4 cut(s) 129, 324, 348, 480
Lsp1109I GCAGC 1 cut(s) 405
MaeIII GTNAC 2 cut(s) 359, 430
MalI GATC 2 cut(s) 123, 512
MboI GATC 2 cut(s) 121, 510
MboII GAAGA 4 cut(s) 33, 208, 231, 249
MflI RGATCY 1 cut(s) 510
MluCI AATT 2 cut(s) 5, 276
MnlI CCTC 6 cut(s) 93, 188, 238, 342, 384, 397
MseI TTAA 3 cut(s) 275, 444, 535
MspA1I CMGCKG 1 cut(s) 418
MwoI GCNNNNNNNGC 2 cut(s) 296, 320
NcoI CCATGG 1 cut(s) 243
NdeII GATC 2 cut(s) 121, 510
NlaIII CATG 1 cut(s) 247
NmuCI GTSAC 1 cut(s) 359
PfeI GAWTC 1 cut(s) 140
PkrI GCNGC 1 cut(s) 420
PpuMI RGGWCCY 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 374
PspPI GGNCC 1 cut(s) 374
PspPPI RGGWCCY 1 cut(s) 374
PsuI RGATCY 1 cut(s) 510
PvuII CAGCTG 1 cut(s) 418
RsaI GTAC 2 cut(s) 59, 136
RsaNI GTAC 2 cut(s) 58, 135
SaqAI TTAA 3 cut(s) 275, 444, 535
SatI GCNGC 1 cut(s) 419
Sau3AI GATC 2 cut(s) 121, 510
Sau96I GGNCC 1 cut(s) 374
SetI ASST 5 cut(s) 158, 272, 348, 376, 420
SinI GGWCC 1 cut(s) 374
Sse9I AATT 2 cut(s) 5, 276
StyI CCWWGG 3 cut(s) 72, 156, 243
TasI AATT 2 cut(s) 5, 276
TatI WGTACW 1 cut(s) 134
TfiI GAWTC 1 cut(s) 140
Tru1I TTAA 3 cut(s) 275, 444, 535
Tru9I TTAA 3 cut(s) 275, 444, 535
TseFI GTSAC 1 cut(s) 359
TseI GCWGC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 359
TspDTI ATGAA 3 cut(s) 17, 101, 249
VpaK11BI GGWCC 1 cut(s) 374
XapI RAATTY 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.