RchiOBHm_Chr3g0472301

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000875
Physical Location & Seq
Forward (+)
18198286 .. 18203233
4948 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43801

Sequence Viewer

Length: 2310 bp
ATGATGGCGAGAAATTTAACACATTATTCCGAACCTTCAGTACAGGACCATGGGTCTCCGCGTAGTAGGAATCCCTCTAGGAAGAGTCAATCTCGAACAGAAAGATCACCTACTCGTCATAGAAAGTCCCACAGAGGCAGTTCTCCACCAAGTGAGAAATATTCAATTCGCCCCAAGTCTCCGAAGCATGCAAGGTCACCCTCAGCTCGCTCTCCTTTTCTCCAGACAAAAAGGTTAAGAAGAGCTGAAGCTGGAGGAGTGCCTGAGAGAGAACAGGAAAGGATCAATGGTAGGGGGATCGGTCGGAGTTTACAGGAAGGTGGTTCTGAGGGAGATGTTGGAAGTGATAGAACGGAGAAATTTTTGGGAAGGAATGATACTGATGGTAATACTTCAAGATCAAGGCATGGTACTTCTCCATCAGATAGAAACCCCAGGAGTAGACATAGACACTCACCTCAACATGCTGGTGATACAAGAGATGAGGAGAGAGTAACTGAGGGCCGTAACAGAAATCATAGCAGAGGGAATGATAGAAGTATGCATAGGGAAAATGAGACTGTAAGAACGGAGAGAATGACAGCAAAAGACAATACTGGTCATATGTCTGCAAGATCAAGGCATGATACTTCTCCATCAGATCAACACCGCAGGAGTAGACATAGATATTACTCACCTCAACATGCTTGTGATACAAGACATGGGGAGAAAGTAATCGAGGGCCGTGAAAGAAATCATAGCAGAGGGAATCATAGAAGTATGCATATGGAAAATGAGACTGAAAGAATGGAGAGAATGACAGCAAGAGAGAGTACTAGTCATCGGTCTTCAAGATCAAGGCATAATACTTCTCCATCAGATCGACACCGCAGTAGACATAGATCTCACTCACCTCAACATACTGGTGATACAAGAGATAAGGAGAAAGTAATTGAGGGCCGTGAAAGAAATCATAGCCGAGGGAATTATAGAAGCATGCGTTGGGAAAATGACACTGAAAGAATGGAGAGAATGACAGCAAAAGAGAGTTCTGGTCATAGGTCTTCAAGATCAAGGCATGGTTCTTCTCCATCAGATCGACACCGCAGCAGTAGACATAGATATCACTCACCTCACCATGCTGGTGATACAAGAGATAAGGAGAAAGTAATTGAAGACTGTCAAAGAAATTATAGGAGAGAGAACGATAGAATTATGCATAGGGAAAAGCATTCAGATAGGAAATATGAGACTGAAAGAATGGAGACAAGGACAGCAAAAGACAGTTCTGGTCATAGGTCTGCAAGAAGACACACGCAATCTACTTCTCCATTGAATGGACACCACAAGAACAGACACCGAACTCATTCATCTCAACAGGTTGCAGATATCAGAGCTCGCAATGAGGCGCCTCAGTCGAGGGAGGCTAAGGATGGGATTGAAAAGAATGATTCAGTAGCTATGATGAGCGCTGCTGAGGAAGTCTTGGAAGCACAGAAAAAGCAAAATCCTTCATTTGAGTTATCTGGAAAGTTTGCTATGGAAACCAATAGATTCAGAGGAGATAGCTATAAGAAGAATCTTCTTTCGAGTAGTAACTATGATGATGATGAAGACTATGATAATGAAAGAACACGAGGCATATGTAGGCTGAATCTAGCACCAAGAAAAAAAAAGCTTCTTGTGTTTGATCTCAATGGCTTACTAGTCTACAGAGTTTTTTGTTTCAACAAGAGTGGTATTCCAAGGGATCGTACTCCTGATGGGAAATGTGGAAACCATCTAGTTTTTAAAAGAGCTTTTGCTGAGGAGTTTATGCTATTCTGCCTTGAAAGATTTGAAGTTGGAATATGGACTTCTGCGCTTGAGAAAAATGTTGATGGTGTCTTGGATTGTGTGATGGAAAAGCTGAGAAGCAGACTTATATTTGTGTGGGATCAGCATCAATGTACTGATTCTGGGTTCAAGTCCCTGGAAACGAAAATGAAGCCTCTGTTTTTCAAAGAACTGAAGAGAGTATGGGATCATTTTGAGGGAAAGTTTTCTGCATCTGATACTCTATTGATTGATGATCAGCCTTACAAGGCCCTTCTCAATCCTCCTTACACAGGGATTTTCCTGGATCCCTACATTCCTGACAATGGTACTGACAATGCTTTAGATCCAAAGAAAGAGTTGGGGAAGTATTTGGCTGGCCTTGCATTTGCAGATGATGTTCAGCATTACGTGAAGGACAATCCCTTTGGCCAGCCTGCAATTTCATCTGAACATCCTGATTGGAATTTTTACTCTCATGTTCTCCGAAAACTTGGAAAGGATCTACTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

769

Amino Acids

89.36

Weight (kDa)

9.9

Isoelectric Point (pI)

64.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 552 - 733 2.2e-19 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1387
AccB7I CCANNNNNTGG 1 cut(s) 1316
AccI GTMKAC 5 cut(s) 442, 658, 874, 1093, 1689
AccII CGCG 1 cut(s) 61
AciI CCGC 4 cut(s) 59, 649, 868, 1084
AclWI GGATC 9 cut(s) 290, 305, 1737, 1923, 2010, 2096, 2109, 2135, 2304
AcoI YGGCCR 1 cut(s) 2224
AcsI RAATTY 3 cut(s) 13, 359, 2260
AcuI CTGAAG 3 cut(s) 21, 267, 2009
AcyI GRCGYC 1 cut(s) 1388
AdeI CACNNNGTG 1 cut(s) 152
AfaI GTAC 6 cut(s) 42, 412, 814, 1735, 1930, 2125
AfeI AGCGCT 1 cut(s) 1450
AfiI CCNNNNNNNGG 3 cut(s) 1316, 2087, 2120
AhdI GACNNNNNGTC 1 cut(s) 52
AhlI ACTAGT 2 cut(s) 815, 1684
AjnI CCWGG 3 cut(s) 434, 1950, 2097
AjuI GAANNNNNNNTTGG 2 cut(s) 964, 996
AluBI AGCT 9 cut(s) 206, 245, 251, 1376, 1439, 1548, 1657, 1778, 1888
AluI AGCT 9 cut(s) 206, 245, 251, 1376, 1439, 1548, 1657, 1778, 1888
Alw21I GWGCWC 1 cut(s) 1378
Alw26I GTCTC 6 cut(s) 60, 183, 551, 770, 1223, 1238
AlwI GGATC 9 cut(s) 290, 305, 1737, 1923, 2010, 2096, 2109, 2135, 2304
Aor51HI AGCGCT 1 cut(s) 1450
AoxI GGCC 6 cut(s) 502, 721, 937, 2064, 2173, 2224
ApeKI GCWGC 2 cut(s) 1086, 1451
ApoI RAATTY 3 cut(s) 13, 359, 2260
ArsI GACNNNNNNTTYG 2 cut(s) 2204, 2236
Asp700I GAANNNNTTC 2 cut(s) 1345, 2020
AspLEI GCGC 3 cut(s) 1390, 1451, 1843
AspS9I GGNCC 5 cut(s) 46, 502, 721, 937, 2065
AvaII GGWCC 1 cut(s) 46
BalI TGGCCA 1 cut(s) 2226
BamHI GGATCC 1 cut(s) 2101
BanI GGYRCC 1 cut(s) 1387
BanII GRGCYC 1 cut(s) 1378
BarI GAAGNNNNNNTAC 2 cut(s) 361, 393
BauI CACGAG 1 cut(s) 1614
BbsI GAAGAC 5 cut(s) 819, 1035, 1161, 1294, 1599
Bbv12I GWGCWC 1 cut(s) 1378
BbvCI CCTCAGC 3 cut(s) 202, 1455, 1785
BbvI GCAGC 2 cut(s) 1098, 1438
BceAI ACGGC 3 cut(s) 489, 708, 924
BciT130I CCWGG 3 cut(s) 436, 1952, 2099
BclI TGATCA 1 cut(s) 2050
BcoDI GTCTC 6 cut(s) 60, 183, 551, 770, 1223, 1238
BcuI ACTAGT 2 cut(s) 815, 1684
BfaI CTAG 5 cut(s) 78, 816, 1637, 1685, 1763
BfmI CTRYAG 1 cut(s) 1690
BfoI RGCGCY 2 cut(s) 1391, 1452
BglII AGATCT 1 cut(s) 881
BisI GCNGC 2 cut(s) 1087, 1452
BlsI GCNGC 2 cut(s) 1088, 1453
BmcAI AGTACT 1 cut(s) 814
Bme1390I CCNGG 3 cut(s) 436, 1952, 2099
Bme18I GGWCC 1 cut(s) 46
BmeRI GACNNNNNGTC 1 cut(s) 52
BmgT120I GGNCC 5 cut(s) 46, 502, 721, 937, 2065
BmiI GGNNCC 2 cut(s) 1389, 2103
BmrFI CCNGG 3 cut(s) 436, 1952, 2099
BmsI GCATC 2 cut(s) 1930, 2036
BpiI GAAGAC 5 cut(s) 819, 1035, 1161, 1294, 1599
BplI GAGNNNNNCTC 2 cut(s) 76, 108
BpmI CTGGAG 2 cut(s) 206, 273
Bpu10I CCTNAGC 4 cut(s) 202, 1407, 1455, 1785
BpuEI CTTGAG 1 cut(s) 1865
BsaAI YACGTR 1 cut(s) 2206
BsaBI GATNNNNATC 1 cut(s) 1920
BsaHI GRCGYC 1 cut(s) 1388
BsaI GGTCTC 1 cut(s) 60
BsaJI CCNNGG 5 cut(s) 49, 434, 958, 1724, 1950
Bsc4I CCNNNNNNNGG 3 cut(s) 1316, 2087, 2120
Bse1I ACTGG 2 cut(s) 601, 907
Bse3DI GCAATG 1 cut(s) 1387
Bse8I GATNNNNATC 1 cut(s) 1920
BseBI CCWGG 3 cut(s) 436, 1952, 2099
BseDI CCNNGG 5 cut(s) 49, 434, 958, 1724, 1950
BseGI GGATG 2 cut(s) 1417, 2248
BseJI GATNNNNATC 1 cut(s) 1920
BseLI CCNNNNNNNGG 3 cut(s) 1316, 2087, 2120
BseMI GCAATG 1 cut(s) 1387
BseMII CTCAG 8 cut(s) 216, 255, 318, 489, 1406, 1446, 1776, 1880
BseNI ACTGG 2 cut(s) 601, 907
BseRI GAGGAG 4 cut(s) 270, 500, 1554, 1802
BseXI GCAGC 2 cut(s) 1098, 1438
Bsh1236I CGCG 1 cut(s) 61
Bsh1285I CGRYCG 1 cut(s) 304
BshFI GGCC 6 cut(s) 504, 723, 939, 2066, 2175, 2226
BshNI GGYRCC 1 cut(s) 1387
BsiEI CGRYCG 1 cut(s) 304
BsiHKAI GWGCWC 1 cut(s) 1378
BslFI GGGAC 2 cut(s) 112, 1933
BslI CCNNNNNNNGG 3 cut(s) 1316, 2087, 2120
BsmAI GTCTC 6 cut(s) 60, 183, 551, 770, 1223, 1238
BsmFI GGGAC 2 cut(s) 112, 1933
BsmI GAATGC 1 cut(s) 1210
BsnI GGCC 6 cut(s) 504, 723, 939, 2066, 2175, 2226
Bso31I GGTCTC 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 1378
Bsp19I CCATGG 1 cut(s) 49
BspACI CCGC 4 cut(s) 59, 649, 868, 1084
BspANI GGCC 6 cut(s) 504, 723, 939, 2066, 2175, 2226
BspCNI CTCAG 8 cut(s) 215, 256, 319, 490, 1405, 1447, 1777, 1881
BspFNI CGCG 1 cut(s) 61
BspLI GGNNCC 2 cut(s) 1389, 2103
BspPI GGATC 9 cut(s) 290, 305, 1737, 1923, 2010, 2096, 2109, 2135, 2304
BspQI GCTCTTC 1 cut(s) 235
BspT107I GGYRCC 1 cut(s) 1387
BspTNI GGTCTC 1 cut(s) 60
BsrDI GCAATG 1 cut(s) 1387
BsrI ACTGG 2 cut(s) 601, 907
BssECI CCNNGG 5 cut(s) 49, 434, 958, 1724, 1950
BssNI GRCGYC 1 cut(s) 1388
BssSI CACGAG 1 cut(s) 1614
BssT1I CCWWGG 2 cut(s) 49, 1724
Bst2BI CACGAG 1 cut(s) 1614
Bst2UI CCWGG 3 cut(s) 436, 1952, 2099
Bst4CI ACNGT 3 cut(s) 562, 1160, 1265
Bst6I CTCTTC 3 cut(s) 77, 235, 1985
BstACI GRCGYC 1 cut(s) 1388
BstBAI YACGTR 1 cut(s) 2206
BstC8I GCNNGC 7 cut(s) 189, 208, 977, 1378, 2173, 2228, 2232
BstDEI CTNAG 9 cut(s) 202, 264, 327, 498, 1392, 1407, 1455, 1785, 1889
BstDSI CCRYGG 1 cut(s) 49
BstEII GGTNACC 1 cut(s) 195
BstENI CCTNNNNNAGG 1 cut(s) 2085
BstF5I GGATG 2 cut(s) 1417, 2248
BstFNI CGCG 1 cut(s) 61
BstH2I RGCGCY 2 cut(s) 1391, 1452
BstHHI GCGC 3 cut(s) 1390, 1451, 1843
BstMAI GTCTC 6 cut(s) 60, 183, 551, 770, 1223, 1238
BstMCI CGRYCG 1 cut(s) 304
BstMWI GCNNNNNNNGC 1 cut(s) 2177
BstNI CCWGG 3 cut(s) 436, 1952, 2099
BstNSI RCATGY 4 cut(s) 191, 467, 686, 979
BstPI GGTNACC 1 cut(s) 195
BstSCI CCNGG 3 cut(s) 434, 1950, 2097
BstSFI CTRYAG 1 cut(s) 1690
BstUI CGCG 1 cut(s) 61
BstV1I GCAGC 2 cut(s) 1098, 1438
BstV2I GAAGAC 5 cut(s) 819, 1035, 1161, 1294, 1599
BstX2I RGATCY 4 cut(s) 881, 2101, 2140, 2296
BstYI RGATCY 4 cut(s) 881, 2101, 2140, 2296
BsuRI GGCC 6 cut(s) 504, 723, 939, 2066, 2175, 2226
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 2 cut(s) 1417, 2248
BtsIMutI CAGTG 1 cut(s) 993
Cac8I GCNNGC 7 cut(s) 189, 208, 977, 1378, 2173, 2228, 2232
CfoI GCGC 3 cut(s) 1390, 1451, 1843
Cfr13I GGNCC 5 cut(s) 46, 502, 721, 937, 2065
Csp6I GTAC 6 cut(s) 41, 411, 813, 1734, 1929, 2124
CspCI CAANNNNNGTGG 2 cut(s) 1696, 1731
CviQI GTAC 6 cut(s) 41, 411, 813, 1734, 1929, 2124
DdeI CTNAG 9 cut(s) 202, 264, 327, 498, 1392, 1407, 1455, 1785, 1889
DinI GGCGCC 1 cut(s) 1389
DraI TTTAAA 1 cut(s) 1771
DraIII CACNNNGTG 1 cut(s) 152
DriI GACNNNNNGTC 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 2224
Eam1104I CTCTTC 3 cut(s) 77, 235, 1985
Eam1105I GACNNNNNGTC 1 cut(s) 52
EarI CTCTTC 3 cut(s) 77, 235, 1985
Ecl136II GAGCTC 1 cut(s) 1376
Eco130I CCWWGG 2 cut(s) 49, 1724
Eco24I GRGCYC 1 cut(s) 1378
Eco31I GGTCTC 1 cut(s) 60
Eco32I GATATC 2 cut(s) 1103, 1369
Eco47I GGWCC 1 cut(s) 46
Eco47III AGCGCT 1 cut(s) 1450
Eco53kI GAGCTC 1 cut(s) 1376
Eco57I CTGAAG 3 cut(s) 21, 267, 2009
Eco91I GGTNACC 1 cut(s) 195
EcoICRI GAGCTC 1 cut(s) 1376
EcoNI CCTNNNNNAGG 1 cut(s) 2085
EcoO109I RGGNCCY 1 cut(s) 2065
EcoO65I GGTNACC 1 cut(s) 195
EcoRII CCWGG 3 cut(s) 434, 1950, 2097
EcoRV GATATC 2 cut(s) 1103, 1369
EcoT14I CCWWGG 2 cut(s) 49, 1724
EcoT22I ATGCAT 3 cut(s) 546, 765, 1200
EcoT38I GRGCYC 1 cut(s) 1378
EgeI GGCGCC 1 cut(s) 1389
EheI GGCGCC 1 cut(s) 1389
ErhI CCWWGG 2 cut(s) 49, 1724
FaqI GGGAC 2 cut(s) 112, 1933
FauNDI CATATG 3 cut(s) 603, 765, 1622
FbaI TGATCA 1 cut(s) 2050
FblI GTMKAC 5 cut(s) 442, 658, 874, 1093, 1689
Fnu4HI GCNGC 2 cut(s) 1087, 1452
FokI GGATG 2 cut(s) 1424, 2235
FriOI GRGCYC 1 cut(s) 1378
Fsp4HI GCNGC 2 cut(s) 1087, 1452
FspBI CTAG 5 cut(s) 78, 816, 1637, 1685, 1763
GlaI GCGC 3 cut(s) 1389, 1450, 1842
GluI GCNGC 2 cut(s) 1087, 1452
GsuI CTGGAG 2 cut(s) 206, 273
HaeII RGCGCY 2 cut(s) 1391, 1452
HaeIII GGCC 6 cut(s) 504, 723, 939, 2066, 2175, 2226
HhaI GCGC 3 cut(s) 1390, 1451, 1843
Hin1I GRCGYC 1 cut(s) 1388
Hin6I GCGC 3 cut(s) 1388, 1449, 1841
HinP1I GCGC 3 cut(s) 1388, 1449, 1841
HindIII AAGCTT 1 cut(s) 1655
HinfI GANTC 8 cut(s) 70, 85, 748, 1430, 1533, 1558, 1633, 1934
Hpy166II GTNNAC 6 cut(s) 311, 443, 659, 875, 1094, 1690
Hpy188III TCNNGA 9 cut(s) 93, 223, 396, 831, 1047, 1506, 1739, 2114, 2252
Hpy8I GTNNAC 6 cut(s) 311, 443, 659, 875, 1094, 1690
HpyAV CCTTC 6 cut(s) 45, 311, 363, 1500, 2078, 2203
HpyCH4III ACNGT 3 cut(s) 562, 1160, 1265
HpyCH4IV ACGT 1 cut(s) 2205
HpyF10VI GCNNNNNNNGC 1 cut(s) 2177
HpyF3I CTNAG 9 cut(s) 202, 264, 327, 498, 1392, 1407, 1455, 1785, 1889
HpySE526I ACGT 1 cut(s) 2205
Hsp92I GRCGYC 1 cut(s) 1388
HspAI GCGC 3 cut(s) 1388, 1449, 1841
KasI GGCGCC 1 cut(s) 1387
Ksp22I TGATCA 1 cut(s) 2050
LguI GCTCTTC 1 cut(s) 235
Lsp1109I GCAGC 2 cut(s) 1098, 1438
LweI GCATC 2 cut(s) 1930, 2036
MaeI CTAG 5 cut(s) 78, 816, 1637, 1685, 1763
MaeII ACGT 1 cut(s) 2205
MaeIII GTNAC 4 cut(s) 195, 493, 506, 1574
MflI RGATCY 4 cut(s) 881, 2101, 2140, 2296
MhlI GDGCHC 1 cut(s) 1378
MlsI TGGCCA 1 cut(s) 2226
MluNI TGGCCA 1 cut(s) 2226
Mly113I GGCGCC 1 cut(s) 1388
MlyI GAGTC 1 cut(s) 94
MmeI TCCRAC 3 cut(s) 284, 319, 1804
Mox20I TGGCCA 1 cut(s) 2226
Mph1103I ATGCAT 3 cut(s) 546, 765, 1200
MroXI GAANNNNTTC 2 cut(s) 1345, 2020
MscI TGGCCA 1 cut(s) 2226
MseI TTAA 4 cut(s) 17, 236, 1770, 2308
MslI CAYNNNNRTG 4 cut(s) 468, 687, 903, 1122
Msp20I TGGCCA 1 cut(s) 2226
MspR9I CCNGG 3 cut(s) 436, 1952, 2099
Mva1269I GAATGC 1 cut(s) 1210
MvaI CCWGG 3 cut(s) 436, 1952, 2099
MvnI CGCG 1 cut(s) 61
MwoI GCNNNNNNNGC 1 cut(s) 2177
NarI GGCGCC 1 cut(s) 1388
NcoI CCATGG 1 cut(s) 49
NdeI CATATG 3 cut(s) 603, 765, 1622
NlaIV GGNNCC 2 cut(s) 1389, 2103
NmeAIII GCCGAG 1 cut(s) 983
NmuCI GTSAC 1 cut(s) 195
NsiI ATGCAT 3 cut(s) 546, 765, 1200
NspI RCATGY 4 cut(s) 191, 467, 686, 979
PaeI GCATGC 2 cut(s) 191, 979
PciSI GCTCTTC 1 cut(s) 235
PctI GAATGC 1 cut(s) 1210
PdmI GAANNNNTTC 2 cut(s) 1345, 2020
PfeI GAWTC 7 cut(s) 70, 748, 1430, 1533, 1558, 1633, 1934
PflMI CCANNNNNTGG 1 cut(s) 1316
PfoI TCCNGGA 1 cut(s) 2097
PkrI GCNGC 2 cut(s) 1088, 1453
PleI GAGTC 1 cut(s) 93
PluTI GGCGCC 1 cut(s) 1391
PpsI GAGTC 1 cut(s) 93
Ppu21I YACGTR 1 cut(s) 2206
Psp124BI GAGCTC 1 cut(s) 1378
Psp6I CCWGG 3 cut(s) 434, 1950, 2097
PspEI GGTNACC 1 cut(s) 195
PspGI CCWGG 3 cut(s) 434, 1950, 2097
PspN4I GGNNCC 2 cut(s) 1389, 2103
PspPI GGNCC 5 cut(s) 46, 502, 721, 937, 2065
PsrI GAACNNNNNNTAC 2 cut(s) 24, 56
PsuI RGATCY 4 cut(s) 881, 2101, 2140, 2296
RsaI GTAC 6 cut(s) 42, 412, 814, 1735, 1930, 2125
RsaNI GTAC 6 cut(s) 41, 411, 813, 1734, 1929, 2124
RseI CAYNNNNRTG 4 cut(s) 468, 687, 903, 1122
SacI GAGCTC 1 cut(s) 1378
SapI GCTCTTC 1 cut(s) 235
SaqAI TTAA 4 cut(s) 17, 236, 1770, 2308
SatI GCNGC 2 cut(s) 1087, 1452
Sau96I GGNCC 5 cut(s) 46, 502, 721, 937, 2065
ScaI AGTACT 1 cut(s) 814
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 3 cut(s) 436, 1952, 2099
SduI GDGCHC 1 cut(s) 1378
SfaNI GCATC 2 cut(s) 1930, 2036
SfcI CTRYAG 1 cut(s) 1690
SfoI GGCGCC 1 cut(s) 1389
SinI GGWCC 1 cut(s) 46
SmiMI CAYNNNNRTG 4 cut(s) 468, 687, 903, 1122
SmlI CTYRAG 1 cut(s) 1844
SmoI CTYRAG 1 cut(s) 1844
SpeI ACTAGT 2 cut(s) 815, 1684
SphI GCATGC 2 cut(s) 191, 979
SsiI CCGC 4 cut(s) 59, 649, 868, 1084
SspDI GGCGCC 1 cut(s) 1387
SspI AATATT 1 cut(s) 161
SspMI CTAG 5 cut(s) 78, 816, 1637, 1685, 1763
SstI GAGCTC 1 cut(s) 1378
StyD4I CCNGG 3 cut(s) 434, 1950, 2097
StyI CCWWGG 2 cut(s) 49, 1724
TaaI ACNGT 3 cut(s) 562, 1160, 1265
TaiI ACGT 1 cut(s) 2208
TaqI TCGA 6 cut(s) 94, 717, 862, 1078, 1397, 1568
TaqII GACCGA 2 cut(s) 290, 813
TatI WGTACW 3 cut(s) 40, 812, 1928
TfiI GAWTC 7 cut(s) 70, 748, 1430, 1533, 1558, 1633, 1934
Tru1I TTAA 4 cut(s) 17, 236, 1770, 2308
Tru9I TTAA 4 cut(s) 17, 236, 1770, 2308
TscAI CASTG 1 cut(s) 1000
TseFI GTSAC 1 cut(s) 195
TseI GCWGC 2 cut(s) 1086, 1451
Tsp45I GTSAC 1 cut(s) 195
TspDTI ATGAA 6 cut(s) 1338, 1482, 1605, 1620, 1979, 2229
TspGWI ACGGA 2 cut(s) 368, 584
TspRI CASTG 1 cut(s) 1000
Van91I CCANNNNNTGG 1 cut(s) 1316
VpaK11BI GGWCC 1 cut(s) 46
XagI CCTNNNNNAGG 1 cut(s) 2085
XapI RAATTY 3 cut(s) 13, 359, 2260
XceI RCATGY 4 cut(s) 191, 467, 686, 979
XmiI GTMKAC 5 cut(s) 442, 658, 874, 1093, 1689
XmnI GAANNNNTTC 2 cut(s) 1345, 2020
XspI CTAG 5 cut(s) 78, 816, 1637, 1685, 1763
ZrmI AGTACT 1 cut(s) 814
Zsp2I ATGCAT 3 cut(s) 546, 765, 1200
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.