Rw4G034000

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000875
Physical Location & Seq
Reverse (-)
61136083 .. 61136907
825 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G034000.1

Sequence Viewer

Length: 825 bp
ATGGCTGGGGAAATTCCTGTGAAGAAGTTTATTTTGTGTGGTGGTGATGATGATGGCGATGATGGCGAATATTACGAAGACACGGGAAGTGAATGTAGCCTTTCACTAGATCAGCTGACTTTAGAACCCAGAAGAAAGAAGCTTCTTGTTTTTGATCTGAATGGGTTACTAGTCTACAGGGTTTATTGTTCCAACAAGCCTCAATTTCCAAGTGATCGTACACAGGATGGGAGATTTGGAAACCATCTAGTTTTCAAAAGACCTTTTGCTGAGGAGTTTGTGCAATTCTGCCTTGAAAGATTTGAGATCGGAATATGGTCTTCTGCCCTAGAGAAAAACGTTGATGCTGTCTTAGAATATTGTATGACGGCAAATCAGAGAAATGGACTATTATTTGTGTGGGGTCAATCTCAATGTACAGATTCTGGGTTCAAGTCCTTGGAGAAAAGAATGAAACCTCTATTTTTCAAAGAACTGAAGAAAGTGTGGTATCATTTTGGCGGAAAGTATTCTAAATGTGATACTTTGTTGATTGATGATCAACCTTACAAGGCCCTACTCAATCCTGCTCACACAGGGATTTTTCTGGAATCCTACAATCCTGACAATGCTAGTGATAATTTATTGGATCCAAATGGACAGCTAGGGGTGTATTTGGATGGCCTTGCGGCTGCGACTAATGCTCAGGTTTATGTAAAGGAGAACCCTATTGGAGTTCCTGCAATTTCATCAGATCATCCTGATTGGAGTTTCTACTCTGAAGTCCTTCGAGGGATTAAGGGAGAAAGTGAGGAAAAGATTACGCCTCAGCAACAAGAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

274

Amino Acids

31.0

Weight (kDa)

4.87

Isoelectric Point (pI)

32.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 47 - 219 1.2e-19 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 174
AciI CCGC 2 cut(s) 501, 668
AclI AACGTT 1 cut(s) 339
AclWI GGATC 2 cut(s) 623, 636
AcsI RAATTY 1 cut(s) 12
AcuI CTGAAG 2 cut(s) 497, 780
AfaI GTAC 2 cut(s) 220, 418
AgsI TTSAA 4 cut(s) 256, 296, 433, 469
AhlI ACTAGT 1 cut(s) 169
AluBI AGCT 3 cut(s) 115, 142, 643
AluI AGCT 3 cut(s) 115, 142, 643
AlwI GGATC 2 cut(s) 623, 636
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 2 cut(s) 552, 661
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 1 cut(s) 12
Asp700I GAANNNNTTC 2 cut(s) 508, 765
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 1 cut(s) 56
BamHI GGATCC 1 cut(s) 628
BbsI GAAGAC 2 cut(s) 84, 312
BbvCI CCTCAGC 2 cut(s) 270, 807
BbvI GCAGC 1 cut(s) 658
BccI CCATC 5 cut(s) 47, 56, 221, 252, 653
BceAI ACGGC 1 cut(s) 384
BclI TGATCA 1 cut(s) 538
BcuI ACTAGT 1 cut(s) 169
BfaI CTAG 6 cut(s) 107, 170, 248, 329, 612, 644
BfmI CTRYAG 1 cut(s) 175
BisI GCNGC 2 cut(s) 669, 672
BlsI GCNGC 2 cut(s) 670, 673
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 630
BmsI GCATC 1 cut(s) 334
BpiI GAAGAC 2 cut(s) 84, 312
Bpu10I CCTNAGC 3 cut(s) 270, 684, 807
BsaJI CCNNGG 1 cut(s) 438
BseDI CCNNGG 1 cut(s) 438
BseGI GGATG 3 cut(s) 232, 664, 736
BseMII CTCAG 3 cut(s) 261, 698, 821
BseRI GAGGAG 1 cut(s) 287
BseXI GCAGC 1 cut(s) 658
BseYI CCCAGC 1 cut(s) 5
BshFI GGCC 2 cut(s) 554, 663
BsnI GGCC 2 cut(s) 554, 663
Bsp1407I TGTACA 1 cut(s) 416
Bsp143I GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
BspACI CCGC 2 cut(s) 501, 668
BspANI GGCC 2 cut(s) 554, 663
BspCNI CTCAG 3 cut(s) 262, 697, 820
BspLI GGNNCC 1 cut(s) 630
BspPI GGATC 2 cut(s) 623, 636
BsrGI TGTACA 1 cut(s) 416
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
BssT1I CCWWGG 1 cut(s) 438
BstAUI TGTACA 1 cut(s) 416
BstDEI CTNAG 4 cut(s) 270, 352, 684, 807
BstF5I GGATG 3 cut(s) 232, 664, 736
BstKTI GATC 7 cut(s) 112, 157, 217, 309, 541, 631, 736
BstMBI GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
BstMWI GCNNNNNNNGC 2 cut(s) 63, 680
BstSFI CTRYAG 1 cut(s) 175
BstV1I GCAGC 1 cut(s) 658
BstV2I GAAGAC 2 cut(s) 84, 312
BstX2I RGATCY 1 cut(s) 628
BstYI RGATCY 1 cut(s) 628
BsuRI GGCC 2 cut(s) 554, 663
BtgZI GCGATG 1 cut(s) 72
BtsCI GGATG 3 cut(s) 232, 664, 736
CaiI CAGNNNCTG 1 cut(s) 425
Cfr13I GGNCC 1 cut(s) 553
Csp6I GTAC 2 cut(s) 219, 417
CviJI RGCY 9 cut(s) 5, 99, 115, 142, 199, 554, 643, 663, 671
CviKI_1 RGCY 9 cut(s) 5, 99, 115, 142, 199, 554, 643, 663, 671
CviQI GTAC 2 cut(s) 219, 417
DdeI CTNAG 4 cut(s) 270, 352, 684, 807
DpnI GATC 7 cut(s) 111, 156, 216, 308, 540, 630, 735
DpnII GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
EciI GGCGGA 1 cut(s) 516
Eco130I CCWWGG 1 cut(s) 438
Eco57I CTGAAG 2 cut(s) 497, 780
EcoO109I RGGNCCY 1 cut(s) 553
EcoT14I CCWWGG 1 cut(s) 438
ErhI CCWWGG 1 cut(s) 438
FaiI YATR 3 cut(s) 316, 365, 693
FbaI TGATCA 1 cut(s) 538
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 2 cut(s) 669, 672
FokI GGATG 3 cut(s) 239, 671, 723
Fsp4HI GCNGC 2 cut(s) 669, 672
FspBI CTAG 6 cut(s) 107, 170, 248, 329, 612, 644
GluI GCNGC 2 cut(s) 669, 672
GsaI CCCAGC 1 cut(s) 9
HaeIII GGCC 2 cut(s) 554, 663
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 3 cut(s) 422, 590, 818
HphI GGTGA 1 cut(s) 56
Hpy166II GTNNAC 2 cut(s) 175, 221
Hpy188I TCNGA 5 cut(s) 159, 311, 378, 733, 760
Hpy188III TCNNGA 4 cut(s) 587, 602, 740, 822
Hpy8I GTNNAC 2 cut(s) 175, 221
HpyAV CCTTC 1 cut(s) 776
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 2 cut(s) 283, 722
HpyF10VI GCNNNNNNNGC 2 cut(s) 63, 680
HpyF3I CTNAG 4 cut(s) 270, 352, 684, 807
HpySE526I ACGT 1 cut(s) 339
Ksp22I TGATCA 1 cut(s) 538
Kzo9I GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 1 cut(s) 334
MaeI CTAG 6 cut(s) 107, 170, 248, 329, 612, 644
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 165
MalI GATC 7 cut(s) 111, 156, 216, 308, 540, 630, 735
MboI GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
MboII GAAGA 5 cut(s) 34, 89, 144, 312, 490
MflI RGATCY 1 cut(s) 628
MluCI AATT 5 cut(s) 12, 203, 284, 619, 723
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 6 cut(s) 210, 265, 468, 764, 784, 816
MroXI GAANNNNTTC 2 cut(s) 508, 765
MseI TTAA 1 cut(s) 777
MspA1I CMGCKG 1 cut(s) 115
MwoI GCNNNNNNNGC 2 cut(s) 63, 680
NdeII GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
NlaIV GGNNCC 1 cut(s) 630
PdmI GAANNNNTTC 2 cut(s) 508, 765
PfeI GAWTC 2 cut(s) 422, 590
PkrI GCNGC 2 cut(s) 670, 673
Psp1406I AACGTT 1 cut(s) 339
PspFI CCCAGC 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 630
PspPI GGNCC 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 628
PvuII CAGCTG 1 cut(s) 115
RsaI GTAC 2 cut(s) 220, 418
RsaNI GTAC 2 cut(s) 219, 417
SaqAI TTAA 1 cut(s) 777
SatI GCNGC 2 cut(s) 669, 672
Sau3AI GATC 7 cut(s) 109, 154, 214, 306, 538, 628, 733
Sau96I GGNCC 1 cut(s) 553
SetI ASST 8 cut(s) 117, 144, 265, 342, 460, 547, 645, 690
SfaNI GCATC 1 cut(s) 334
SfcI CTRYAG 1 cut(s) 175
SpeI ACTAGT 1 cut(s) 169
Sse9I AATT 5 cut(s) 12, 203, 284, 619, 723
SsiI CCGC 2 cut(s) 501, 668
SspI AATATT 2 cut(s) 71, 359
SspMI CTAG 6 cut(s) 107, 170, 248, 329, 612, 644
StyI CCWWGG 1 cut(s) 438
TaiI ACGT 1 cut(s) 342
TaqI TCGA 1 cut(s) 769
TasI AATT 5 cut(s) 12, 203, 284, 619, 723
TatI WGTACW 1 cut(s) 416
TauI GCSGC 1 cut(s) 671
TfiI GAWTC 2 cut(s) 422, 590
Tru1I TTAA 1 cut(s) 777
Tru9I TTAA 1 cut(s) 777
TseI GCWGC 1 cut(s) 671
TspDTI ATGAA 2 cut(s) 467, 717
XapI RAATTY 1 cut(s) 12
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 2 cut(s) 508, 765
XspI CTAG 6 cut(s) 107, 170, 248, 329, 612, 644
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.