Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr14

Orthogroup ID

OG0000875

Physical Location & Seq

Genomic Coordinates Reverse (-)

1243927 .. 1245956

Gene Structure 2030 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD14G1014000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 798 bp

ATGGCTGATGAAAAGATCTGTAAAAACAAGCAACAATATGACAGCGATGACGGTGACGATGGCAACGAAGTCACCGGAAGCGATTGCGGCCTTTCTCTGGAGAAGCTAAACATAGGATCAAGGAAGAAGCTTGTAATCTTTAGTCTCAATGGGTTTATAGTCCACAGGGTTTCCATCCACAACAAGACAAAAATTCCGCGCAATCGTACTCCTGATGGCACATATGGAAGCTATCTAGTTTTTAAGAGACCATTTTGTGAGGAATTCATGCAATTTTGTTTTGAAAGATTTGAAGTTGGAATATGGAGTTCTGCCCAAGAAAAAAATGTGGATGGTGTGTTGGATTGTATAATGGGAAAGCTGAGAAAACGACTGGCATTTGTTTGGAATAAAAACAAGCCCCTGTTTTTCGAAGAACTGGACAAACTGTGGAGCTATTTTAGTGGGAAATATTCAGAATCTGATACACTTTTCATTGATGATCAACCTTATAAGGCTCTATTGAATCCTGTATGGTCATCACAACTCTGCCTTCCTTTTTTGAATCAATTGACAGAAATGGAAGGAATGCAGAAGGGGAAAACGAGAGTCCTAACACAGGGATCTTCTTGGGGTCATATAATGCTGAATCCAAAGGGAGAACTTGGGAAGTATTTGGATGGCCTTGCAGATGCAAAAGATGTGCAAATTTATGTGAAGGGAAACCCTTTTGGAATGCCTGCAATTTCACCTACTAATCCGGATTGGAAATTCTACTCGAATGTTCTTCGTAGACTTGGAAAGCGGTACAAGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NIF

Protein Analysis

266

Amino Acids

30.48

Weight (kDa)

8.97

Isoelectric Point (pI)

36.74

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NIF	PF03031	43 - 170	1.1e-14	NLI interacting factor-like phosphatase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0000875)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G36540 AT2G36550
fragaria_vesca	FvH4_6g17650 FvH4_6g17650 FvH4_6g17680 FvH4_6g18040 FvH4_6g18040 FvH4_6g18041
malus_domestica	MD14G1013900.v1.1 MD14G1014000.v1.1 MD14G1014100.v1.1
prunus_persica	Prupe.7G119400_v2.0.a1 Prupe.7G119500_v2.0.a1 Prupe.7G119500_v2.0.a1
pyrus_communis	pycom14g01180 pycom14g01190
rosa_chinensis	RchiOBHm_Chr3g0471881 RchiOBHm_Chr3g0471901 RchiOBHm_Chr3g0472301 RchiOBHm_Chr3g0472311 RchiOBHm_Chr4g0442541
rosa_laevigata	RLG00000006003 RLG00000024088 RLG00000024089 RLG00000024121
rosa_multiflora	Rmu_sc0000627.1_g000001 Rmu_sc0002115.1_g000024 Rmu_sc0016565.1_g000001 Rmu_sc0036882.1_g000001 Rmu_ssc0000204.1_g000046
rosa_roxburghii	Rroxscaffold_5G00383360 Rroxscaffold_6G00409040 Rroxscaffold_6G00409080 Rroxscaffold_6G00409090 Rroxscaffold_6G00409430 Rroxscaffold_6G00409450 Rroxscaffold_6G00410650
rosa_rugosa	Rorug03G0111000 Rorug03G0111100 Rorug03G0121100 Rorug03G0123800 Rorug03G0123900 Rorug03G0124100 Rorug04G0341200
rosa_samantha	Rh3AG172300 Rh3AG172400 Rh3AG172500 Rh3AG176100 Rh3AG176200 Rh3BG197800 Rh3BG197900 Rh3BG201800 Rh3BG201900 Rh3CG188800 Rh3DG173400 Rh3DG173500 Rh3DG176400 Rh3DG176600 Rh4AG393500 Rh4BG406600 Rh4CG422000
rosa_wichuraiana	Rw3G015210 Rw3G016510 Rw3G016520 Rw3G016820 Rw4G034000

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	492
AccI	GTMKAC	1 cut(s)	772
AccII	CGCG	1 cut(s)	199
AccIII	TCCGGA	1 cut(s)	739
AciI	CCGC	3 cut(s)	87, 197, 784
AclWI	GGATC	2 cut(s)	124, 610
AcsI	RAATTY	4 cut(s)	192, 263, 687, 749
AfaI	GTAC	2 cut(s)	208, 788
AfiI	CCNNNNNNNGG	2 cut(s)	97, 598
AgsI	TTSAA	4 cut(s)	284, 293, 505, 544
AluBI	AGCT	5 cut(s)	106, 130, 231, 361, 435
AluI	AGCT	5 cut(s)	106, 130, 231, 361, 435
Alw26I	GTCTC	2 cut(s)	149, 241
AlwI	GGATC	2 cut(s)	124, 610
AlwNI	CAGNNNCTG	1 cut(s)	461
Aor13HI	TCCGGA	1 cut(s)	739
AoxI	GGCC	2 cut(s)	88, 661
ApoI	RAATTY	4 cut(s)	192, 263, 687, 749
AspLEI	GCGC	1 cut(s)	201
AsuHPI	GGTGA	3 cut(s)	64, 65, 720
AsuII	TTCGAA	1 cut(s)	411
BaeI	ACNNNNGTAYC	1 cut(s)	778
BccI	CCATC	5 cut(s)	53, 182, 209, 326, 653
BclI	TGATCA	1 cut(s)	481
BcoDI	GTCTC	2 cut(s)	149, 241
BfaI	CTAG	1 cut(s)	236
BglII	AGATCT	1 cut(s)	15
BisI	GCNGC	1 cut(s)	88
BlsI	GCNGC	1 cut(s)	89
BmsI	GCATC	1 cut(s)	661
BpmI	CTGGAG	1 cut(s)	119
Bpu14I	TTCGAA	1 cut(s)	411
BsaI	GGTCTC	1 cut(s)	241
BsaWI	WCCGGW	2 cut(s)	74, 739
Bsc4I	CCNNNNNNNGG	2 cut(s)	97, 598
Bse1I	ACTGG	2 cut(s)	378, 423
BseAI	TCCGGA	1 cut(s)	739
BseGI	GGATG	3 cut(s)	174, 337, 664
BseLI	CCNNNNNNNGG	2 cut(s)	97, 598
BseMII	CTCAG	1 cut(s)	353
BseNI	ACTGG	2 cut(s)	378, 423
Bsh1236I	CGCG	1 cut(s)	199
BshFI	GGCC	2 cut(s)	90, 663
BsiSI	CCGG	2 cut(s)	75, 740
BslI	CCNNNNNNNGG	2 cut(s)	97, 598
BsmAI	GTCTC	2 cut(s)	149, 241
BsmI	GAATGC	2 cut(s)	573, 720
BsnI	GGCC	2 cut(s)	90, 663
Bso31I	GGTCTC	1 cut(s)	241
Bsp119I	TTCGAA	1 cut(s)	411
Bsp13I	TCCGGA	1 cut(s)	739
Bsp143I	GATC	4 cut(s)	15, 116, 481, 602
BspACI	CCGC	3 cut(s)	87, 197, 784
BspANI	GGCC	2 cut(s)	90, 663
BspCNI	CTCAG	1 cut(s)	354
BspEI	TCCGGA	1 cut(s)	739
BspFNI	CGCG	1 cut(s)	199
BspPI	GGATC	2 cut(s)	124, 610
BspT104I	TTCGAA	1 cut(s)	411
BspTNI	GGTCTC	1 cut(s)	241
BsrI	ACTGG	2 cut(s)	378, 423
BssMI	GATC	4 cut(s)	15, 116, 481, 602
Bst4CI	ACNGT	2 cut(s)	53, 429
BstBI	TTCGAA	1 cut(s)	411
BstC8I	GCNNGC	1 cut(s)	720
BstDEI	CTNAG	1 cut(s)	362
BstENI	CCTNNNNNAGG	1 cut(s)	596
BstF5I	GGATG	3 cut(s)	174, 337, 664
BstFNI	CGCG	1 cut(s)	199
BstHHI	GCGC	1 cut(s)	201
BstKTI	GATC	4 cut(s)	18, 119, 484, 605
BstMAI	GTCTC	2 cut(s)	149, 241
BstMBI	GATC	4 cut(s)	15, 116, 481, 602
BstMWI	GCNNNNNNNGC	2 cut(s)	87, 790
BstUI	CGCG	1 cut(s)	199
BstX2I	RGATCY	2 cut(s)	15, 602
BstYI	RGATCY	2 cut(s)	15, 602
BsuRI	GGCC	2 cut(s)	90, 663
BtgZI	GCGATG	1 cut(s)	60
BtsCI	GGATG	3 cut(s)	174, 337, 664
Cac8I	GCNNGC	1 cut(s)	720
CaiI	CAGNNNCTG	1 cut(s)	461
CfoI	GCGC	1 cut(s)	201
Csp6I	GTAC	2 cut(s)	207, 787
CviAII	CATG	1 cut(s)	268
CviQI	GTAC	2 cut(s)	207, 787
DdeI	CTNAG	1 cut(s)	362
DpnI	GATC	4 cut(s)	17, 118, 483, 604
DpnII	GATC	4 cut(s)	15, 116, 481, 602
Eco31I	GGTCTC	1 cut(s)	241
EcoNI	CCTNNNNNAGG	1 cut(s)	596
EcoRI	GAATTC	1 cut(s)	263
FaeI	CATG	1 cut(s)	271
FatI	CATG	1 cut(s)	267
FauNDI	CATATG	1 cut(s)	223
FbaI	TGATCA	1 cut(s)	481
FblI	GTMKAC	1 cut(s)	772
Fnu4HI	GCNGC	1 cut(s)	88
FokI	GGATG	3 cut(s)	161, 344, 671
Fsp4HI	GCNGC	1 cut(s)	88
FspBI	CTAG	1 cut(s)	236
GlaI	GCGC	1 cut(s)	200
GluI	GCNGC	1 cut(s)	88
GsuI	CTGGAG	1 cut(s)	119
HaeIII	GGCC	2 cut(s)	90, 663
HapII	CCGG	2 cut(s)	75, 740
HhaI	GCGC	1 cut(s)	201
Hin1II	CATG	1 cut(s)	271
Hin6I	GCGC	1 cut(s)	199
HinP1I	GCGC	1 cut(s)	199
HindIII	AAGCTT	1 cut(s)	128
HinfI	GANTC	5 cut(s)	458, 505, 544, 588, 628
HpaII	CCGG	2 cut(s)	75, 740
HphI	GGTGA	3 cut(s)	64, 65, 720
Hpy166II	GTNNAC	2 cut(s)	163, 773
Hpy188I	TCNGA	2 cut(s)	457, 463
Hpy188III	TCNNGA	3 cut(s)	98, 212, 740
Hpy8I	GTNNAC	2 cut(s)	163, 773
HpyAV	CCTTC	4 cut(s)	542, 557, 568, 691
HpyCH4III	ACNGT	2 cut(s)	53, 429
HpyCH4V	TGCA	6 cut(s)	271, 571, 668, 674, 685, 722
HpyF10VI	GCNNNNNNNGC	2 cut(s)	87, 790
HpyF3I	CTNAG	1 cut(s)	362
Hsp92II	CATG	1 cut(s)	271
HspAI	GCGC	1 cut(s)	199
Kpn2I	TCCGGA	1 cut(s)	739
Ksp22I	TGATCA	1 cut(s)	481
Kzo9I	GATC	4 cut(s)	15, 116, 481, 602
LmnI	GCTCC	1 cut(s)	432
LweI	GCATC	1 cut(s)	661
MaeI	CTAG	1 cut(s)	236
MaeIII	GTNAC	2 cut(s)	53, 70
MalI	GATC	4 cut(s)	17, 118, 483, 604
MboI	GATC	4 cut(s)	15, 116, 481, 602
MboII	GAAGA	4 cut(s)	136, 425, 597, 758
MfeI	CAATTG	1 cut(s)	548
MflI	RGATCY	2 cut(s)	15, 602
MluCI	AATT	7 cut(s)	192, 263, 272, 548, 687, 723, 749
MlyI	GAGTC	1 cut(s)	597
MmeI	TCCRAC	2 cut(s)	277, 321
MnlI	CCTC	1 cut(s)	253
MroI	TCCGGA	1 cut(s)	739
MseI	TTAA	1 cut(s)	243
MspI	CCGG	2 cut(s)	75, 740
MunI	CAATTG	1 cut(s)	548
Mva1269I	GAATGC	2 cut(s)	573, 720
MvnI	CGCG	1 cut(s)	199
MwoI	GCNNNNNNNGC	2 cut(s)	87, 790
NdeI	CATATG	1 cut(s)	223
NdeII	GATC	4 cut(s)	15, 116, 481, 602
NlaIII	CATG	1 cut(s)	271
NmuCI	GTSAC	2 cut(s)	53, 70
NspV	TTCGAA	1 cut(s)	411
PcsI	WCGNNNNNNNCGW	1 cut(s)	63
PctI	GAATGC	2 cut(s)	573, 720
PfeI	GAWTC	4 cut(s)	458, 505, 544, 628
PkrI	GCNGC	1 cut(s)	89
PleI	GAGTC	1 cut(s)	596
PpsI	GAGTC	1 cut(s)	596
PsiI	TTATAA	1 cut(s)	492
PstNI	CAGNNNCTG	1 cut(s)	461
PsuI	RGATCY	2 cut(s)	15, 602
RsaI	GTAC	2 cut(s)	208, 788
RsaNI	GTAC	2 cut(s)	207, 787
SaqAI	TTAA	1 cut(s)	243
SatI	GCNGC	1 cut(s)	88
Sau3AI	GATC	4 cut(s)	15, 116, 481, 602
SchI	GAGTC	1 cut(s)	597
SetI	ASST	7 cut(s)	108, 132, 233, 363, 437, 490, 733
SfaNI	GCATC	1 cut(s)	661
SfuI	TTCGAA	1 cut(s)	411
Sse9I	AATT	7 cut(s)	192, 263, 272, 548, 687, 723, 749
SsiI	CCGC	3 cut(s)	87, 197, 784
SspI	AATATT	1 cut(s)	452
SspMI	CTAG	1 cut(s)	236
TaaI	ACNGT	2 cut(s)	53, 429
TaqI	TCGA	2 cut(s)	411, 758
TasI	AATT	7 cut(s)	192, 263, 272, 548, 687, 723, 749
TauI	GCSGC	1 cut(s)	90
TfiI	GAWTC	4 cut(s)	458, 505, 544, 628
Tru1I	TTAA	1 cut(s)	243
Tru9I	TTAA	1 cut(s)	243
TseFI	GTSAC	2 cut(s)	53, 70
Tsp45I	GTSAC	2 cut(s)	53, 70
TspDTI	ATGAA	3 cut(s)	24, 256, 463
XagI	CCTNNNNNAGG	1 cut(s)	596
XapI	RAATTY	4 cut(s)	192, 263, 687, 749
XmiI	GTMKAC	1 cut(s)	772
XspI	CTAG	1 cut(s)	236

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.