RchiOBHm_Chr3g0472311

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0000875
Physical Location & Seq
Forward (+)
18203932 .. 18205221
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43802

Sequence Viewer

Length: 861 bp
ATGGCTGAAAAGAATAAAAGCAAAGTTGTGTGCTCAGATGATGATCACAGTGATGCTGAAGAGAGCAAAAAGACATTGGATGACTTAACTCTCCCTCTAGAGAAACTCAACCTTGGCCCAGAAAAGAAGAAGAAGAAGAAGCTTCTGGTTATTGGTCTTGGAGGCTTGCTTTGTCATAGAGTATACCGCACTGAAAAAATCTACATTCCAAATTATCGCCGTCCGGATGCAGCATATGGAAACTACAAGCGTATAAATTGCTTTTATAATATAAATTCTTTCCATTACATGTCTCATTTTGTAGTTTGTTCAATAAGAGCTCTTTTCTGTTTGATCTTGATAACAGTTTACAAGAGGCCTTACTGTGAAGAATTTATGAAATGGTACTTGGACAATGCCTTGGATTGTGGGATGAAAGGATTGAGGAGGAAGTTGTTGTTTGCTTGGGATCAAGTGGAGTGTACTGATTCAGGGTTCAAAACCTTGGAGAACCAAAAGAAGCCCATCTTTCTGAAAGGGTTGAAGAAAATATGGGAAAACAAGGATTTCAAAGCCTCACTTCCATCTTCCATGGGGAAATACTCTTCATTGAACACCTTGTTAATTGATGATAAGGCTTACAAGGCTCTACTAAATCCTGCTCACACAGCCATCTTTCCTCCTGAATTCAAGGTTGACCAAGTTGATGACAAGGCTTTAGGTCCTAATGGTGAGTTGAGGCTTTACTTGGAGGGACTTGCAGATGCCGATGATGTTACTTCTTATGTTAAAGATCATCCATTTGGACAGCCAGCAATAACAACTACTCACTCAGATTGGGATTTCTATTCAAAGATCCTTACTCATTTCCAGAAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

286

Amino Acids

32.99

Weight (kDa)

8.7

Isoelectric Point (pI)

33.65

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 267
AccI GTMKAC 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 223
AciI CCGC 1 cut(s) 187
AclWI GGATC 2 cut(s) 456, 829
AcsI RAATTY 3 cut(s) 274, 371, 665
AcuI CTGAAG 1 cut(s) 78
AfaI GTAC 2 cut(s) 386, 463
AflIII ACRYGT 1 cut(s) 288
AgsI TTSAA 7 cut(s) 312, 478, 523, 550, 592, 670, 831
AjuI GAANNNNNNNTTGG 2 cut(s) 371, 403
AluBI AGCT 2 cut(s) 142, 320
AluI AGCT 2 cut(s) 142, 320
Alw21I GWGCWC 2 cut(s) 35, 322
Alw26I GTCTC 1 cut(s) 297
AlwI GGATC 2 cut(s) 456, 829
Aor13HI TCCGGA 1 cut(s) 223
AoxI GGCC 2 cut(s) 115, 356
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 3 cut(s) 274, 371, 665
AspS9I GGNCC 2 cut(s) 116, 701
AsuHPI GGTGA 1 cut(s) 722
AvaII GGWCC 1 cut(s) 701
BanII GRGCYC 1 cut(s) 322
Bbv12I GWGCWC 2 cut(s) 35, 322
BbvI GCAGC 1 cut(s) 242
BccI CCATC 3 cut(s) 512, 571, 659
BceAI ACGGC 1 cut(s) 204
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 297
BfaI CTAG 1 cut(s) 98
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme18I GGWCC 1 cut(s) 701
BmgT120I GGNCC 2 cut(s) 116, 701
BmsI GCATC 3 cut(s) 43, 217, 733
BsaBI GATNNNNATC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 112, 399, 483, 570
BsaWI WCCGGW 1 cut(s) 223
Bse8I GATNNNNATC 1 cut(s) 42
BseAI TCCGGA 1 cut(s) 223
BseDI CCNNGG 4 cut(s) 112, 399, 483, 570
BseGI GGATG 4 cut(s) 85, 232, 417, 775
BseJI GATNNNNATC 1 cut(s) 42
BseMII CTCAG 2 cut(s) 48, 825
BseRI GAGGAG 1 cut(s) 439
BseXI GCAGC 1 cut(s) 242
BshFI GGCC 2 cut(s) 117, 358
BsiHKAI GWGCWC 2 cut(s) 35, 322
BsiSI CCGG 1 cut(s) 224
BslFI GGGAC 1 cut(s) 747
BsmAI GTCTC 1 cut(s) 297
BsmFI GGGAC 1 cut(s) 747
BsnI GGCC 2 cut(s) 117, 358
Bsp1286I GDGCHC 2 cut(s) 35, 322
Bsp13I TCCGGA 1 cut(s) 223
Bsp143I GATC 5 cut(s) 43, 333, 448, 772, 834
Bsp19I CCATGG 1 cut(s) 570
BspACI CCGC 1 cut(s) 187
BspANI GGCC 2 cut(s) 117, 358
BspCNI CTCAG 2 cut(s) 47, 824
BspEI TCCGGA 1 cut(s) 223
BspPI GGATC 2 cut(s) 456, 829
BssECI CCNNGG 4 cut(s) 112, 399, 483, 570
BssMI GATC 5 cut(s) 43, 333, 448, 772, 834
BssNAI GTATAC 1 cut(s) 184
BssT1I CCWWGG 4 cut(s) 112, 399, 483, 570
Bst1107I GTATAC 1 cut(s) 184
Bst4CI ACNGT 3 cut(s) 50, 346, 365
Bst6I CTCTTC 2 cut(s) 54, 589
BstC8I GCNNGC 2 cut(s) 167, 792
BstDEI CTNAG 2 cut(s) 34, 811
BstDSI CCRYGG 1 cut(s) 570
BstF5I GGATG 4 cut(s) 85, 232, 417, 775
BstKTI GATC 5 cut(s) 46, 336, 451, 775, 837
BstMAI GTCTC 1 cut(s) 297
BstMBI GATC 5 cut(s) 43, 333, 448, 772, 834
BstMWI GCNNNNNNNGC 2 cut(s) 623, 647
BstNSI RCATGY 1 cut(s) 292
BstV1I GCAGC 1 cut(s) 242
BstX2I RGATCY 1 cut(s) 834
BstYI RGATCY 1 cut(s) 834
BstZ17I GTATAC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 117, 358
BtgI CCRYGG 1 cut(s) 570
BtsCI GGATG 4 cut(s) 85, 232, 417, 775
BtsIMutI CAGTG 2 cut(s) 55, 189
Cac8I GCNNGC 2 cut(s) 167, 792
Cfr13I GGNCC 2 cut(s) 116, 701
Csp6I GTAC 2 cut(s) 385, 462
CviAII CATG 2 cut(s) 289, 571
CviQI GTAC 2 cut(s) 385, 462
DdeI CTNAG 2 cut(s) 34, 811
DpnI GATC 5 cut(s) 45, 335, 450, 774, 836
DpnII GATC 5 cut(s) 43, 333, 448, 772, 834
Eam1104I CTCTTC 2 cut(s) 54, 589
EarI CTCTTC 2 cut(s) 54, 589
Ecl136II GAGCTC 1 cut(s) 320
Eco130I CCWWGG 4 cut(s) 112, 399, 483, 570
Eco147I AGGCCT 1 cut(s) 358
Eco24I GRGCYC 1 cut(s) 322
Eco47I GGWCC 1 cut(s) 701
Eco53kI GAGCTC 1 cut(s) 320
Eco57I CTGAAG 1 cut(s) 78
EcoICRI GAGCTC 1 cut(s) 320
EcoO109I RGGNCCY 1 cut(s) 701
EcoRI GAATTC 1 cut(s) 665
EcoT14I CCWWGG 4 cut(s) 112, 399, 483, 570
EcoT38I GRGCYC 1 cut(s) 322
ErhI CCWWGG 4 cut(s) 112, 399, 483, 570
FaeI CATG 2 cut(s) 292, 574
FalI AAGNNNNNCTT 4 cut(s) 491, 523, 543, 575
FaqI GGGAC 1 cut(s) 747
FatI CATG 2 cut(s) 288, 570
FauNDI CATATG 1 cut(s) 235
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 4 cut(s) 92, 239, 424, 762
FriOI GRGCYC 1 cut(s) 322
Fsp4HI GCNGC 1 cut(s) 231
FspBI CTAG 1 cut(s) 98
GluI GCNGC 1 cut(s) 231
HaeIII GGCC 2 cut(s) 117, 358
HapII CCGG 1 cut(s) 224
Hin1II CATG 2 cut(s) 292, 574
HincII GTYRAC 1 cut(s) 676
HindII GTYRAC 1 cut(s) 676
HindIII AAGCTT 1 cut(s) 140
HinfI GANTC 1 cut(s) 467
HpaII CCGG 1 cut(s) 224
HphI GGTGA 1 cut(s) 722
Hpy166II GTNNAC 4 cut(s) 184, 349, 462, 676
Hpy188I TCNGA 3 cut(s) 37, 513, 814
Hpy188III TCNNGA 5 cut(s) 98, 224, 337, 662, 850
Hpy8I GTNNAC 4 cut(s) 184, 349, 462, 676
HpyCH4III ACNGT 3 cut(s) 50, 346, 365
HpyCH4V TGCA 2 cut(s) 230, 740
HpyF10VI GCNNNNNNNGC 2 cut(s) 623, 647
HpyF3I CTNAG 2 cut(s) 34, 811
Hsp92II CATG 2 cut(s) 292, 574
Kpn2I TCCGGA 1 cut(s) 223
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 5 cut(s) 43, 333, 448, 772, 834
LpnPI CCDG 7 cut(s) 131, 132, 237, 456, 651, 675, 804
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 3 cut(s) 43, 217, 733
MaeI CTAG 1 cut(s) 98
MaeIII GTNAC 1 cut(s) 754
MalI GATC 5 cut(s) 45, 335, 450, 774, 836
MboI GATC 5 cut(s) 43, 333, 448, 772, 834
MboII GAAGA 9 cut(s) 71, 139, 142, 145, 148, 380, 535, 558, 576
MflI RGATCY 1 cut(s) 834
MhlI GDGCHC 2 cut(s) 35, 322
MluCI AATT 6 cut(s) 211, 256, 274, 371, 603, 665
MnlI CCTC 9 cut(s) 105, 155, 348, 417, 420, 565, 669, 711, 724
MroI TCCGGA 1 cut(s) 223
MseI TTAA 4 cut(s) 86, 602, 768, 859
MslI CAYNNNNRTG 1 cut(s) 51
MspI CCGG 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 623, 647
NcoI CCATGG 1 cut(s) 570
NdeI CATATG 1 cut(s) 235
NdeII GATC 5 cut(s) 43, 333, 448, 772, 834
NlaIII CATG 2 cut(s) 292, 574
NspI RCATGY 1 cut(s) 292
PceI AGGCCT 1 cut(s) 358
PciI ACATGT 1 cut(s) 288
PfeI GAWTC 1 cut(s) 467
PkrI GCNGC 1 cut(s) 232
PpuMI RGGWCCY 1 cut(s) 701
PscI ACATGT 1 cut(s) 288
PsiI TTATAA 1 cut(s) 267
Psp124BI GAGCTC 1 cut(s) 322
Psp5II RGGWCCY 1 cut(s) 701
PspPI GGNCC 2 cut(s) 116, 701
PspPPI RGGWCCY 1 cut(s) 701
PsuI RGATCY 1 cut(s) 834
RsaI GTAC 2 cut(s) 386, 463
RsaNI GTAC 2 cut(s) 385, 462
RseI CAYNNNNRTG 1 cut(s) 51
SacI GAGCTC 1 cut(s) 322
SaqAI TTAA 4 cut(s) 86, 602, 768, 859
SatI GCNGC 1 cut(s) 231
Sau3AI GATC 5 cut(s) 43, 333, 448, 772, 834
Sau96I GGNCC 2 cut(s) 116, 701
SduI GDGCHC 2 cut(s) 35, 322
SetI ASST 7 cut(s) 114, 144, 322, 485, 599, 675, 703
SfaNI GCATC 3 cut(s) 43, 217, 733
SinI GGWCC 1 cut(s) 701
SmiMI CAYNNNNRTG 1 cut(s) 51
Sse9I AATT 6 cut(s) 211, 256, 274, 371, 603, 665
SseBI AGGCCT 1 cut(s) 358
SsiI CCGC 1 cut(s) 187
SspMI CTAG 1 cut(s) 98
SstI GAGCTC 1 cut(s) 322
StuI AGGCCT 1 cut(s) 358
StyI CCWWGG 4 cut(s) 112, 399, 483, 570
TaaI ACNGT 3 cut(s) 50, 346, 365
TasI AATT 6 cut(s) 211, 256, 274, 371, 603, 665
TatI WGTACW 1 cut(s) 461
TfiI GAWTC 1 cut(s) 467
Tru1I TTAA 4 cut(s) 86, 602, 768, 859
Tru9I TTAA 4 cut(s) 86, 602, 768, 859
TscAI CASTG 2 cut(s) 55, 196
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 392, 428, 576
TspRI CASTG 2 cut(s) 55, 196
VpaK11BI GGWCC 1 cut(s) 701
XapI RAATTY 3 cut(s) 274, 371, 665
XbaI TCTAGA 1 cut(s) 97
XceI RCATGY 1 cut(s) 292
XmiI GTMKAC 1 cut(s) 183
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.