Rorug03G0124100

NLI interacting factor-like phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000875
Physical Location & Seq
Reverse (-)
10024566 .. 10030628
6063 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0124100.1

Sequence Viewer

Length: 2244 bp
ATGGCTACTACCATAGCTCATTCGGCGCTGGCGTTTACTGCAGCGGCTTCCGGTTCGGCTTCGGATGGGTTCAGGAGCTCGAGTAATGGAGTTCCTCTCAGGGCCCTAGGGAAGGCCAGGTTCAGCGTGAAGAGGAACCTTGCGGTCTCGGCCAAGCTCAGGAAGGTGAAGAAGCACGACTATCCATGGCCGGCCGATCCGGATCCGAATGTCAAAGGAGGAGTGCTTACTCATCTCTCTCACTTCAAGCCGCTCAAAGAGAAGCCCAAGCCGGTTACTCTGCCTTTCGAGAAGCCTCTCGTTGAAATCGAAAAGAAGATCATTGATGTGCGGAAGATGGCTAACGAAACTGGACTGGACTTCAGTGATCAAATTATTTCATTGGAGAATAAACACCAACAGGCTTTAAAGGATTTATATTTGAATCTCACTCCTATACAACGCGTAAATATTGCACGACATCCTAACAGGCCAACTTTCCTTGATCACGTGTTTAACATTACTGATACGTTTGTAGAGCTTCATGGAGACCGAGCAGGGTATGATGATCCTGCTATTGTCACTGGTATAGGAACTATAGATGGTAATAGGTATATGTTTATGGGTCACCAGAAAGGTAGAAATACGAAGGAAAACATTCAGCGTAACTTTGGGATGCCTACTCCTCATGGTTACCGGAAGGCTCTGCGCATGATGTACTACGCAGATCACCATGGGTTCCCCATTGTTACTTTCATTGATACTCCTGGTGCATATGCAGACCTTAAATCTGAGGAGTTGGGCCAAGGTGAAGCCATAGCTCACAACTTGCGGACTATGTTCGGTCTGAAGGTACCAATTGTTTCTATCGTCATTGGGGAAGGTGGATCTGGTGGTGCCCTTGCCATTGGCTGTGCTAATAAACTATTAATGCTTGAAAATGCAGTTTTTTATGTTGCCAGTCCTGAAGCATGTGCAGCAATCTTGTGGAAGAGTGCCAAAGCATCTCCAAAGGCAGCCGAGAAGCTGAAAATTACTGCAACTGAGTTGTGCAAGCTGCAAATTGCAGACGGCATCATCCCTGAGCCGCTCGGTGGTGCACATGCAGATTCCTCGTGGACCTCTCAACAGATCAAAAATGCAATTAACAAAGAAATGGATGAGCTTAAGAAGATGGACACTGAAGCACTATTAAAGCATCGCATGATGAAGTTCCGCAAAATTGGTGGGTTCCAAGAAGGAATCCCTATAGATCCAAAGAAGAAAAGGAACATGAAACAGAAAGAGGAACCCAAGGTTTCAGTTGTGGATTTAGAGGGTGAAGTTGAAAAGGTCAAACTGCAAATTGAGGAGGCAAAGAAATCCTCTGATAAGCCTGCATTGTTACCTCTGACTGAGGCAATAGAAAAACTGAAAAGGGAGGTTGATAATGAATTTTCTGAGGCAGTTAAAGCCCTAGGCTTGAAAGACAGGTTTGAGTCATTGCAGGAAGAATTTTTGAAAATAAGTTCACAGGACCAACTCATGCATCCAGCTCTGAAAGAAAAGATTCAAAAGCTTAGGCATGAATTTACCCAGAGCCTGTCATCTGCTCCTAATTATGAAGACCTAAAGTATAAACTTGACATGCTGAAGGAATTATCACAAGCATATAAATACGAGGAGAAGAACAACAAAGCTGCAACATTGAAGCAGGAAGTTAACAATAAATTTCACGAAATCATGGAACGGCCAGATGTTAAGGAGAAAACTGCAGCACTTAGGGCTGAGATTGAAAATGCCGGGGTGTCTAACCTTGATAATTTGGATGACAACCTGAAGGGGAAGATTTTGGAATTGAAGAAAGAGTTGGACTTCGAATTTGTTGGTGTCCTCAAGTCCTTGGGGTTGGATGTTGAGATAAGATCAAAAGAACCAATTGAGCAAATTTTGCCCTCAGAAGTCAAGACCAAGATTGAAGAATTGAATGAAGAAATTAATCACAAGATTGAAAATGTTATCAATTCATCAGACTTGAAGGACAAGATTGAGTTACTAAAGTTGGAAGTAGCGAAGGCAGGTAAGACGCCTGATTCAGCAACAAAAAACAGAATTGTGGCTTTGGAGGAACAAATCAAACAAAGCTTGGCAGCAGCTGTTGAGTCATCAAACCTAAAAGAGAAGCACGAGAAGCTTAAGGCTGAGGTTTCCAAAATCATTGAATCTTCTGGAGAATCAGATGGAAGTTTGAAAAAGGACAATCCAAATGAAGTCTATGTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

747

Amino Acids

83.37

Weight (kDa)

8.24

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ACCA PF03255 94 - 402 4.3e-132 Acetyl co-enzyme A carboxylase carboxyltransferase-like
Carboxyl_trans PF01039 172 - 333 1.5e-07 Carboxyl transferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 689
Acc36I ACCTGC 1 cut(s) 2027
Acc65I GGTACC 1 cut(s) 832
AccB1I GGYRCC 2 cut(s) 832, 875
AccBSI CCGCTC 2 cut(s) 253, 1069
AccII CGCG 1 cut(s) 444
AccIII TCCGGA 1 cut(s) 199
AciI CCGC 7 cut(s) 44, 143, 251, 331, 811, 1067, 1195
AclWI GGATC 6 cut(s) 191, 197, 210, 542, 874, 1226
AcoI YGGCCR 4 cut(s) 150, 188, 192, 1709
AcsI RAATTY 6 cut(s) 1412, 1472, 1547, 1688, 1838, 1905
AcuI CTGAAG 6 cut(s) 346, 848, 966, 1182, 1631, 1817
AcvI CACGTG 1 cut(s) 490
AcyI GRCGYC 1 cut(s) 2045
AfaI GTAC 2 cut(s) 698, 834
AfiI CCNNNNNNNGG 3 cut(s) 112, 159, 1073
AflII CTTAAG 2 cut(s) 1145, 2153
AflIII ACRYGT 2 cut(s) 442, 489
AjnI CCWGG 2 cut(s) 116, 745
AjuI GAANNNNNNNTTGG 2 cut(s) 1811, 1843
Alw21I GWGCWC 2 cut(s) 80, 1081
Alw26I GTCTC 2 cut(s) 151, 522
Alw44I GTGCAC 1 cut(s) 1077
AlwI GGATC 6 cut(s) 191, 197, 210, 542, 874, 1226
AlwNI CAGNNNCTG 2 cut(s) 1561, 2114
Ama87I CYCGRG 1 cut(s) 79
Aor13HI TCCGGA 1 cut(s) 199
AoxI GGCC 8 cut(s) 102, 114, 150, 188, 192, 470, 781, 1709
ApaI GGGCCC 1 cut(s) 106
ApaLI GTGCAC 1 cut(s) 1077
ApeKI GCWGC 8 cut(s) 41, 956, 995, 1036, 1658, 1733, 2108, 2111
ApoI RAATTY 6 cut(s) 1412, 1472, 1547, 1688, 1838, 1905
ArsI GACNNNNNNTTYG 2 cut(s) 1823, 1855
AseI ATTAAT 2 cut(s) 908, 1956
Asp700I GAANNNNTTC 2 cut(s) 636, 1527
Asp718I GGTACC 1 cut(s) 832
AspA2I CCTAGG 2 cut(s) 106, 1435
AspLEI GCGC 2 cut(s) 28, 690
AspS9I GGNCC 5 cut(s) 102, 103, 781, 1098, 1495
AsuC2I CCSGG 1 cut(s) 1762
AsuHPI GGTGA 5 cut(s) 178, 599, 701, 800, 1310
AsuII TTCGAA 1 cut(s) 1836
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 2 cut(s) 1098, 1495
AvrII CCTAGG 2 cut(s) 106, 1435
BaeGI GKGCMC 3 cut(s) 106, 880, 1081
BaeI ACNNNNGTAYC 2 cut(s) 498, 531
BamHI GGATCC 1 cut(s) 202
BanI GGYRCC 2 cut(s) 832, 875
BanII GRGCYC 2 cut(s) 80, 106
BauI CACGAG 2 cut(s) 1093, 2144
BbrPI CACGTG 1 cut(s) 490
BbsI GAAGAC 1 cut(s) 1590
Bbv12I GWGCWC 2 cut(s) 80, 1081
BbvCI CCTCAGC 1 cut(s) 2160
BbvI GCAGC 8 cut(s) 53, 968, 1007, 1023, 1645, 1745, 2120, 2123
BccI CCATC 5 cut(s) 59, 331, 575, 1147, 2192
BceAI ACGGC 2 cut(s) 1066, 1724
BcgI CGANNNNNNTGC 2 cut(s) 1074, 1108
BciT130I CCWGG 2 cut(s) 118, 747
BclI TGATCA 2 cut(s) 367, 484
BcnI CCSGG 1 cut(s) 1762
BcoDI GTCTC 2 cut(s) 151, 522
BfaI CTAG 2 cut(s) 107, 1436
BfmI CTRYAG 4 cut(s) 39, 576, 1227, 1731
BfoI RGCGCY 1 cut(s) 29
BfrI CTTAAG 2 cut(s) 1145, 2153
BfuAI ACCTGC 1 cut(s) 2027
BlnI CCTAGG 2 cut(s) 106, 1435
Bme1390I CCNGG 3 cut(s) 118, 747, 1762
Bme18I GGWCC 2 cut(s) 1098, 1495
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 5 cut(s) 102, 103, 781, 1098, 1495
BmiI GGNNCC 8 cut(s) 104, 137, 204, 719, 834, 877, 1211, 1269
BmrFI CCNGG 3 cut(s) 118, 747, 1762
BmsI GCATC 5 cut(s) 645, 992, 1062, 1186, 1516
BpiI GAAGAC 1 cut(s) 1590
BplI GAGNNNNNCTC 2 cut(s) 81, 113
BpmI CTGGAG 1 cut(s) 2208
Bpu10I CCTNAGC 4 cut(s) 158, 1062, 1538, 2160
Bpu14I TTCGAA 1 cut(s) 1836
BpuEI CTTGAG 1 cut(s) 1838
BpuMI CCSGG 1 cut(s) 1762
BsaAI YACGTR 1 cut(s) 490
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 2045
BsaI GGTCTC 2 cut(s) 151, 522
BsaJI CCNNGG 8 cut(s) 106, 185, 712, 784, 1272, 1435, 1761, 1860
BsaWI WCCGGW 3 cut(s) 50, 199, 675
BsaXI ACNNNNNCTCC 2 cut(s) 67, 97
Bsc4I CCNNNNNNNGG 3 cut(s) 112, 159, 1073
Bse118I RCCGGY 2 cut(s) 190, 271
Bse1I ACTGG 4 cut(s) 355, 360, 568, 939
Bse3DI GCAATG 1 cut(s) 1460
Bse8I GATNNNNATC 1 cut(s) 201
BseAI TCCGGA 1 cut(s) 199
BseBI CCWGG 2 cut(s) 118, 747
BseDI CCNNGG 8 cut(s) 106, 185, 712, 784, 1272, 1435, 1761, 1860
BseGI GGATG 8 cut(s) 70, 460, 660, 1056, 1144, 1507, 1792, 1876
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 112, 159, 1073
BseMI GCAATG 1 cut(s) 1460
BseNI ACTGG 4 cut(s) 355, 360, 568, 939
BseRI GAGGAG 5 cut(s) 234, 654, 788, 1343, 1655
BseSI GKGCMC 3 cut(s) 106, 880, 1081
BseX3I CGGCCG 1 cut(s) 192
BseXI GCAGC 8 cut(s) 53, 968, 1007, 1023, 1645, 1745, 2120, 2123
BsgI GTGCAG 1 cut(s) 975
Bsh1236I CGCG 1 cut(s) 444
Bsh1285I CGRYCG 1 cut(s) 195
BshFI GGCC 8 cut(s) 104, 116, 152, 190, 194, 472, 783, 1711
BshNI GGYRCC 2 cut(s) 832, 875
BsiEI CGRYCG 1 cut(s) 195
BsiHKAI GWGCWC 2 cut(s) 80, 1081
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 6 cut(s) 51, 191, 200, 272, 676, 1761
BslI CCNNNNNNNGG 3 cut(s) 112, 159, 1073
BsmAI GTCTC 2 cut(s) 151, 522
BsnI GGCC 8 cut(s) 104, 116, 152, 190, 194, 472, 783, 1711
Bso31I GGTCTC 2 cut(s) 151, 522
BsoBI CYCGRG 1 cut(s) 79
Bsp119I TTCGAA 1 cut(s) 1836
Bsp120I GGGCCC 1 cut(s) 102
Bsp1286I GDGCHC 4 cut(s) 80, 106, 880, 1081
Bsp13I TCCGGA 1 cut(s) 199
Bsp19I CCATGG 2 cut(s) 185, 712
BspACI CCGC 7 cut(s) 44, 143, 251, 331, 811, 1067, 1195
BspANI GGCC 8 cut(s) 104, 116, 152, 190, 194, 472, 783, 1711
BspEI TCCGGA 1 cut(s) 199
BspFNI CGCG 1 cut(s) 444
BspLI GGNNCC 8 cut(s) 104, 137, 204, 719, 834, 877, 1211, 1269
BspMAI CTGCAG 2 cut(s) 43, 1735
BspMI ACCTGC 1 cut(s) 2027
BspPI GGATC 6 cut(s) 191, 197, 210, 542, 874, 1226
BspT104I TTCGAA 1 cut(s) 1836
BspT107I GGYRCC 2 cut(s) 832, 875
BspTI CTTAAG 2 cut(s) 1145, 2153
BspTNI GGTCTC 2 cut(s) 151, 522
BsrBI CCGCTC 2 cut(s) 253, 1069
BsrDI GCAATG 1 cut(s) 1460
BsrFI RCCGGY 2 cut(s) 190, 271
BsrI ACTGG 4 cut(s) 355, 360, 568, 939
BssAI RCCGGY 2 cut(s) 190, 271
BssECI CCNNGG 8 cut(s) 106, 185, 712, 784, 1272, 1435, 1761, 1860
BssNI GRCGYC 1 cut(s) 2045
BssSI CACGAG 2 cut(s) 1093, 2144
BssT1I CCWWGG 7 cut(s) 106, 185, 712, 784, 1272, 1435, 1860
Bst2BI CACGAG 2 cut(s) 1093, 2144
Bst2UI CCWGG 2 cut(s) 118, 747
Bst6I CTCTTC 2 cut(s) 125, 965
BstACI GRCGYC 1 cut(s) 2045
BstAFI CTTAAG 2 cut(s) 1145, 2153
BstAPI GCANNNNNTGC 1 cut(s) 1909
BstBAI YACGTR 1 cut(s) 490
BstBI TTCGAA 1 cut(s) 1836
BstC8I GCNNGC 4 cut(s) 30, 192, 1034, 1356
BstDSI CCRYGG 2 cut(s) 185, 712
BstEII GGTNACC 2 cut(s) 605, 671
BstENI CCTNNNNNAGG 1 cut(s) 110
BstF5I GGATG 8 cut(s) 70, 460, 660, 1056, 1144, 1507, 1792, 1876
BstFNI CGCG 1 cut(s) 444
BstH2I RGCGCY 1 cut(s) 29
BstHHI GCGC 2 cut(s) 28, 690
BstMAI GTCTC 2 cut(s) 151, 522
BstMCI CGRYCG 1 cut(s) 195
BstMWI GCNNNNNNNGC 8 cut(s) 23, 38, 149, 956, 1430, 1742, 1909, 2149
BstNI CCWGG 2 cut(s) 118, 747
BstNSI RCATGY 3 cut(s) 954, 1085, 1609
BstPI GGTNACC 2 cut(s) 605, 671
BstSCI CCNGG 3 cut(s) 116, 745, 1760
BstSFI CTRYAG 4 cut(s) 39, 576, 1227, 1731
BstSLI GKGCMC 3 cut(s) 106, 880, 1081
BstUI CGCG 1 cut(s) 444
BstV1I GCAGC 8 cut(s) 53, 968, 1007, 1023, 1645, 1745, 2120, 2123
BstV2I GAAGAC 1 cut(s) 1590
BstX2I RGATCY 3 cut(s) 202, 866, 1231
BstYI RGATCY 3 cut(s) 202, 866, 1231
BstZI CGGCCG 1 cut(s) 192
BsuRI GGCC 8 cut(s) 104, 116, 152, 190, 194, 472, 783, 1711
BtgI CCRYGG 2 cut(s) 185, 712
BtgZI GCGATG 1 cut(s) 1163
BtsCI GGATG 8 cut(s) 70, 460, 660, 1056, 1144, 1507, 1792, 1876
BtsIMutI CAGTG 3 cut(s) 370, 561, 1158
BveI ACCTGC 1 cut(s) 2027
Cac8I GCNNGC 4 cut(s) 30, 192, 1034, 1356
CaiI CAGNNNCTG 2 cut(s) 1561, 2114
CfoI GCGC 2 cut(s) 28, 690
Cfr10I RCCGGY 2 cut(s) 190, 271
Cfr13I GGNCC 5 cut(s) 102, 103, 781, 1098, 1495
CseI GACGC 1 cut(s) 2053
Csp6I GTAC 2 cut(s) 697, 833
CspCI CAANNNNNGTGG 2 cut(s) 1186, 1221
CviQI GTAC 2 cut(s) 697, 833
DraI TTTAAA 1 cut(s) 408
EaeI YGGCCR 4 cut(s) 150, 188, 192, 1709
EagI CGGCCG 1 cut(s) 192
Eam1104I CTCTTC 2 cut(s) 125, 965
EarI CTCTTC 2 cut(s) 125, 965
Ecl136II GAGCTC 1 cut(s) 78
EclXI CGGCCG 1 cut(s) 192
Eco130I CCWWGG 7 cut(s) 106, 185, 712, 784, 1272, 1435, 1860
Eco24I GRGCYC 2 cut(s) 80, 106
Eco31I GGTCTC 2 cut(s) 151, 522
Eco47I GGWCC 2 cut(s) 1098, 1495
Eco52I CGGCCG 1 cut(s) 192
Eco53kI GAGCTC 1 cut(s) 78
Eco57I CTGAAG 6 cut(s) 346, 848, 966, 1182, 1631, 1817
Eco72I CACGTG 1 cut(s) 490
Eco88I CYCGRG 1 cut(s) 79
Eco91I GGTNACC 2 cut(s) 605, 671
EcoICRI GAGCTC 1 cut(s) 78
EcoNI CCTNNNNNAGG 1 cut(s) 110
EcoO109I RGGNCCY 2 cut(s) 102, 103
EcoO65I GGTNACC 2 cut(s) 605, 671
EcoRII CCWGG 2 cut(s) 116, 745
EcoT14I CCWWGG 7 cut(s) 106, 185, 712, 784, 1272, 1435, 1860
EcoT22I ATGCAT 1 cut(s) 1509
EcoT38I GRGCYC 2 cut(s) 80, 106
ErhI CCWWGG 7 cut(s) 106, 185, 712, 784, 1272, 1435, 1860
FauNDI CATATG 1 cut(s) 754
FbaI TGATCA 2 cut(s) 367, 484
FokI GGATG 8 cut(s) 77, 447, 667, 1043, 1151, 1494, 1799, 1883
FriOI GRGCYC 2 cut(s) 80, 106
FseI GGCCGGCC 1 cut(s) 194
FspBI CTAG 2 cut(s) 107, 1436
FspI TGCGCA 1 cut(s) 689
GlaI GCGC 2 cut(s) 27, 689
GsuI CTGGAG 1 cut(s) 2208
HaeII RGCGCY 1 cut(s) 29
HaeIII GGCC 8 cut(s) 104, 116, 152, 190, 194, 472, 783, 1711
HapII CCGG 6 cut(s) 51, 191, 200, 272, 676, 1761
HgaI GACGC 1 cut(s) 2053
HhaI GCGC 2 cut(s) 28, 690
Hin1I GRCGYC 1 cut(s) 2045
Hin6I GCGC 2 cut(s) 26, 688
HinP1I GCGC 2 cut(s) 26, 688
HincII GTYRAC 1 cut(s) 1681
HindII GTYRAC 1 cut(s) 1681
HindIII AAGCTT 3 cut(s) 1535, 2101, 2150
HinfI GANTC 9 cut(s) 424, 1088, 1221, 1457, 1528, 2051, 2120, 2180, 2192
HpaI GTTAAC 1 cut(s) 1681
HpaII CCGG 6 cut(s) 51, 191, 200, 272, 676, 1761
HphI GGTGA 5 cut(s) 178, 599, 701, 800, 1310
Hpy166II GTNNAC 5 cut(s) 36, 1079, 1098, 1490, 1681
Hpy188III TCNNGA 8 cut(s) 73, 160, 200, 289, 944, 1694, 1924, 2187
Hpy8I GTNNAC 5 cut(s) 36, 1079, 1098, 1490, 1681
HpyCH4IV ACGT 2 cut(s) 489, 509
HpyF10VI GCNNNNNNNGC 8 cut(s) 23, 38, 149, 956, 1430, 1742, 1909, 2149
HpySE526I ACGT 2 cut(s) 489, 509
Hsp92I GRCGYC 1 cut(s) 2045
HspAI GCGC 2 cut(s) 26, 688
Kpn2I TCCGGA 1 cut(s) 199
KpnI GGTACC 1 cut(s) 836
KroI GCCGGC 1 cut(s) 190
KroNI GCCGGC 1 cut(s) 192
Ksp22I TGATCA 2 cut(s) 367, 484
KspAI GTTAAC 1 cut(s) 1681
LmnI GCTCC 2 cut(s) 75, 1576
Lsp1109I GCAGC 8 cut(s) 53, 968, 1007, 1023, 1645, 1745, 2120, 2123
LweI GCATC 5 cut(s) 645, 992, 1062, 1186, 1516
MaeI CTAG 2 cut(s) 107, 1436
MaeII ACGT 2 cut(s) 489, 509
MaeIII GTNAC 8 cut(s) 274, 559, 605, 644, 671, 727, 1362, 2010
MbiI CCGCTC 2 cut(s) 253, 1069
MfeI CAATTG 2 cut(s) 837, 1896
MflI RGATCY 3 cut(s) 202, 866, 1231
MhlI GDGCHC 4 cut(s) 80, 106, 880, 1081
MluI ACGCGT 1 cut(s) 442
MlyI GAGTC 2 cut(s) 1466, 2129
MmeI TCCRAC 3 cut(s) 1809, 1848, 2001
Mph1103I ATGCAT 1 cut(s) 1509
MroI TCCGGA 1 cut(s) 199
MroNI GCCGGC 1 cut(s) 190
MroXI GAANNNNTTC 2 cut(s) 636, 1527
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 2 cut(s) 44, 2114
MspCI CTTAAG 2 cut(s) 1145, 2153
MspI CCGG 6 cut(s) 51, 191, 200, 272, 676, 1761
MspR9I CCNGG 3 cut(s) 118, 747, 1762
MunI CAATTG 2 cut(s) 837, 1896
MvaI CCWGG 2 cut(s) 118, 747
MvnI CGCG 1 cut(s) 444
MwoI GCNNNNNNNGC 8 cut(s) 23, 38, 149, 956, 1430, 1742, 1909, 2149
NaeI GCCGGC 1 cut(s) 192
NciI CCSGG 1 cut(s) 1762
NcoI CCATGG 2 cut(s) 185, 712
NdeI CATATG 1 cut(s) 754
NgoMIV GCCGGC 1 cut(s) 190
NlaIV GGNNCC 8 cut(s) 104, 137, 204, 719, 834, 877, 1211, 1269
NmeAIII GCCGAG 2 cut(s) 128, 1024
NmuCI GTSAC 2 cut(s) 559, 605
NsbI TGCGCA 1 cut(s) 689
NsiI ATGCAT 1 cut(s) 1509
NspI RCATGY 3 cut(s) 954, 1085, 1609
NspV TTCGAA 1 cut(s) 1836
PaeR7I CTCGAG 1 cut(s) 79
PcsI WCGNNNNNNNCGW 2 cut(s) 29, 306
PdiI GCCGGC 1 cut(s) 192
PdmI GAANNNNTTC 2 cut(s) 636, 1527
PfeI GAWTC 7 cut(s) 424, 1088, 1221, 1528, 2051, 2180, 2192
PleI GAGTC 2 cut(s) 1465, 2128
PmaCI CACGTG 1 cut(s) 490
PmlI CACGTG 1 cut(s) 490
PpsI GAGTC 2 cut(s) 1465, 2128
Ppu21I YACGTR 1 cut(s) 490
PshBI ATTAAT 2 cut(s) 908, 1956
Psp124BI GAGCTC 1 cut(s) 80
Psp6I CCWGG 2 cut(s) 116, 745
PspCI CACGTG 1 cut(s) 490
PspEI GGTNACC 2 cut(s) 605, 671
PspGI CCWGG 2 cut(s) 116, 745
PspN4I GGNNCC 8 cut(s) 104, 137, 204, 719, 834, 877, 1211, 1269
PspOMI GGGCCC 1 cut(s) 102
PspPI GGNCC 5 cut(s) 102, 103, 781, 1098, 1495
PspXI VCTCGAGB 1 cut(s) 79
PstI CTGCAG 2 cut(s) 43, 1735
PstNI CAGNNNCTG 2 cut(s) 1561, 2114
PsuI RGATCY 3 cut(s) 202, 866, 1231
PvuII CAGCTG 1 cut(s) 2114
RigI GGCCGGCC 1 cut(s) 194
RsaI GTAC 2 cut(s) 698, 834
RsaNI GTAC 2 cut(s) 697, 833
RseI CAYNNNNRTG 1 cut(s) 326
SacI GAGCTC 1 cut(s) 80
Sau96I GGNCC 5 cut(s) 102, 103, 781, 1098, 1495
SchI GAGTC 2 cut(s) 1466, 2129
ScrFI CCNGG 3 cut(s) 118, 747, 1762
SduI GDGCHC 4 cut(s) 80, 106, 880, 1081
SfaNI GCATC 5 cut(s) 645, 992, 1062, 1186, 1516
SfcI CTRYAG 4 cut(s) 39, 576, 1227, 1731
Sfr274I CTCGAG 1 cut(s) 79
SfuI TTCGAA 1 cut(s) 1836
SinI GGWCC 2 cut(s) 1098, 1495
SlaI CTCGAG 1 cut(s) 79
SmiMI CAYNNNNRTG 1 cut(s) 326
SmlI CTYRAG 4 cut(s) 79, 1145, 1853, 2153
SmoI CTYRAG 4 cut(s) 79, 1145, 1853, 2153
SsiI CCGC 7 cut(s) 44, 143, 251, 331, 811, 1067, 1195
SspI AATATT 1 cut(s) 451
SspMI CTAG 2 cut(s) 107, 1436
SstI GAGCTC 1 cut(s) 80
StyD4I CCNGG 3 cut(s) 116, 745, 1760
StyI CCWWGG 7 cut(s) 106, 185, 712, 784, 1272, 1435, 1860
TaiI ACGT 2 cut(s) 492, 512
TaqI TCGA 4 cut(s) 80, 288, 309, 1836
TaqII GACCGA 2 cut(s) 546, 812
TatI WGTACW 1 cut(s) 696
TauI GCSGC 3 cut(s) 47, 253, 1069
TfiI GAWTC 7 cut(s) 424, 1088, 1221, 1528, 2051, 2180, 2192
TscAI CASTG 3 cut(s) 370, 568, 1165
TseFI GTSAC 2 cut(s) 559, 605
TseI GCWGC 8 cut(s) 41, 956, 995, 1036, 1658, 1733, 2108, 2111
Tsp45I GTSAC 2 cut(s) 559, 605
TspRI CASTG 3 cut(s) 370, 568, 1165
Vha464I CTTAAG 2 cut(s) 1145, 2153
VneI GTGCAC 1 cut(s) 1077
VpaK11BI GGWCC 2 cut(s) 1098, 1495
VspI ATTAAT 2 cut(s) 908, 1956
XagI CCTNNNNNAGG 1 cut(s) 110
XapI RAATTY 6 cut(s) 1412, 1472, 1547, 1688, 1838, 1905
XceI RCATGY 3 cut(s) 954, 1085, 1609
XhoI CTCGAG 1 cut(s) 79
XmaJI CCTAGG 2 cut(s) 106, 1435
XmnI GAANNNNTTC 2 cut(s) 636, 1527
XspI CTAG 2 cut(s) 107, 1436
Zsp2I ATGCAT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.