Rroxscaffold_6G00409430

catalytic domain of ctd-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000875
Physical Location & Seq
Forward (+)
31926508 .. 31928321
1814 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409430.1

Sequence Viewer

Length: 975 bp
ATGAAGAATTTCCACGGGCGCGGTCCTTTTCAGTGCCCCTATATGATGAACCATAAAAGTTGGAAGAAATTCTATCTTGATTATGAATTCTGTGACAGTGATGATGAACACCTTTTTTACCTTGGAATCTGTACTCCTGCTGATCTTAACAATGATAACAAAGAAACAGGAAGTGACTCTGGCCTTTCAGTACTAGAGAAGCTAAGTCTCCAACCACGAAAGAAGCTTCTTGTTCTCAGTCTCAATGGGCTTCTACTCTACCGGGTTTACAGGAAAAACAAGGCTAAATTCCCAACTACTCGAGAACCTGATGCCAGATTTGCAAGTCAACTGGTTTTCAAAAGACCTTTTGCTGCAGAGTTTGTGGAATTCTGCCTTGAACGATTTGAAAAAAGTCAAAGGTGTTTTGAATCGCGTAATGGGAAGGAGGCAGAGAAAGAAACTGCTCAAGACGATTGCACTTATTCTGGGTCCATGTCCGTGGAGGATAAAGAGAAGCCCTTGTTTCTCAAAGAATTGAAGGATTTGTGGAAATATCTTGATGGGAAGTATTCTGAATCTGATACAATGTTGATTGATGATCAACCTTACAAGGCCTTGCTCAATCCTCCTTACATAGGGAGTTTCCTCGAATCCTACAATCCTGACAATGCTGGTGATAATGCATTAGGAGAACTGGGGGTGTACTTGGATGACCTTGCAGCTGCAGATAATGTTCAGGTTTATGTGAAAAAGAACCCATTTGGAGTTCCTGCAATTTCATCTGGCCATCCTGATTGGAGCTTATACTTTGATGTCATTCGACAACTTAAGGGAGAGAGCGAGAAAGCAAGTACATCTCGATCAACAGCTGGATTCTTGAGTCTTCCTAGTATCAGCTGGATTCAAACTTTCTTGTTGTATTCACTTGGAATTTGTTGTAAAACTATAGTAAACGAATTTGAGAGAGAGAGAGAGTATAGACGTAGAGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NIF

Protein Analysis

324

Amino Acids

37.33

Weight (kDa)

5.64

Isoelectric Point (pI)

35.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NIF PF03031 75 - 239 4.1e-11 NLI interacting factor-like phosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 21, 415
AciI CCGC 1 cut(s) 21
AcoI YGGCCR 1 cut(s) 766
AcsI RAATTY 7 cut(s) 7, 68, 86, 287, 368, 912, 938
AfaI GTAC 4 cut(s) 133, 192, 686, 835
AfiI CCNNNNNNNGG 1 cut(s) 617
AflII CTTAAG 1 cut(s) 809
AgsI TTSAA 6 cut(s) 340, 380, 389, 410, 520, 887
AluBI AGCT 6 cut(s) 202, 226, 704, 783, 851, 879
AluI AGCT 6 cut(s) 202, 226, 704, 783, 851, 879
Alw26I GTCTC 2 cut(s) 212, 245
Ama87I CYCGRG 1 cut(s) 300
AoxI GGCC 3 cut(s) 181, 594, 766
ApeKI GCWGC 3 cut(s) 353, 701, 704
ApoI RAATTY 7 cut(s) 7, 68, 86, 287, 368, 912, 938
Asp700I GAANNNNTTC 2 cut(s) 8, 68
AspLEI GCGC 1 cut(s) 21
AspS9I GGNCC 2 cut(s) 23, 471
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 668
AvaI CYCGRG 1 cut(s) 300
AvaII GGWCC 2 cut(s) 23, 471
BaeGI GKGCMC 1 cut(s) 38
BalI TGGCCA 1 cut(s) 768
BbsI GAAGAC 1 cut(s) 857
BbvI GCAGC 3 cut(s) 340, 691, 713
BccI CCATC 2 cut(s) 536, 777
BclI TGATCA 1 cut(s) 580
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 2 cut(s) 212, 245
BfaI CTAG 2 cut(s) 194, 870
BfmI CTRYAG 3 cut(s) 354, 705, 927
BfrI CTTAAG 1 cut(s) 809
BisI GCNGC 3 cut(s) 354, 702, 705
BlsI GCNGC 3 cut(s) 355, 703, 706
BmcAI AGTACT 1 cut(s) 192
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 2 cut(s) 23, 471
BmeT110I CYCGRG 1 cut(s) 300
BmgT120I GGNCC 2 cut(s) 23, 471
BmiI GGNNCC 1 cut(s) 472
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 1 cut(s) 686
BmsI GCATC 1 cut(s) 301
BmuI ACTGGG 1 cut(s) 686
BpiI GAAGAC 1 cut(s) 857
BpuEI CTTGAG 2 cut(s) 432, 880
BpuMI CCSGG 1 cut(s) 263
BsaJI CCNNGG 3 cut(s) 13, 121, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 617
Bse1I ACTGG 2 cut(s) 336, 681
BseDI CCNNGG 3 cut(s) 13, 121, 480
BseGI GGATG 2 cut(s) 697, 769
BseLI CCNNNNNNNGG 1 cut(s) 617
BseMII CTCAG 1 cut(s) 250
BseNI ACTGG 2 cut(s) 336, 681
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 3 cut(s) 340, 691, 713
Bsh1236I CGCG 2 cut(s) 21, 415
BshFI GGCC 3 cut(s) 183, 596, 768
BsiHKCI CYCGRG 1 cut(s) 300
BsiSI CCGG 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 617
BsmAI GTCTC 2 cut(s) 212, 245
BsnI GGCC 3 cut(s) 183, 596, 768
BsoBI CYCGRG 1 cut(s) 300
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 3 cut(s) 142, 580, 842
BspACI CCGC 1 cut(s) 21
BspANI GGCC 3 cut(s) 183, 596, 768
BspCNI CTCAG 1 cut(s) 249
BspFNI CGCG 2 cut(s) 21, 415
BspLI GGNNCC 1 cut(s) 472
BspMAI CTGCAG 2 cut(s) 358, 709
BspTI CTTAAG 1 cut(s) 809
BsrI ACTGG 2 cut(s) 336, 681
BssECI CCNNGG 3 cut(s) 13, 121, 480
BssMI GATC 3 cut(s) 142, 580, 842
BssT1I CCWWGG 1 cut(s) 121
Bst4CI ACNGT 1 cut(s) 98
BstAFI CTTAAG 1 cut(s) 809
BstDEI CTNAG 2 cut(s) 203, 236
BstDSI CCRYGG 2 cut(s) 13, 480
BstENI CCTNNNNNAGG 1 cut(s) 615
BstF5I GGATG 2 cut(s) 697, 769
BstFNI CGCG 2 cut(s) 21, 415
BstHHI GCGC 1 cut(s) 21
BstKTI GATC 3 cut(s) 145, 583, 845
BstMAI GTCTC 2 cut(s) 212, 245
BstMBI GATC 3 cut(s) 142, 580, 842
BstMWI GCNNNNNNNGC 1 cut(s) 320
BstSCI CCNGG 1 cut(s) 261
BstSFI CTRYAG 3 cut(s) 354, 705, 927
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 2 cut(s) 21, 415
BstV1I GCAGC 3 cut(s) 340, 691, 713
BstV2I GAAGAC 1 cut(s) 857
BstXI CCANNNNNNTGG 1 cut(s) 481
BsuRI GGCC 3 cut(s) 183, 596, 768
BtgI CCRYGG 2 cut(s) 13, 480
BtsCI GGATG 2 cut(s) 697, 769
BtsIMutI CAGTG 2 cut(s) 38, 103
CfoI GCGC 1 cut(s) 21
Cfr13I GGNCC 2 cut(s) 23, 471
Csp6I GTAC 4 cut(s) 132, 191, 685, 834
CviAII CATG 1 cut(s) 475
CviQI GTAC 4 cut(s) 132, 191, 685, 834
DdeI CTNAG 2 cut(s) 203, 236
DpnI GATC 3 cut(s) 144, 582, 844
DpnII GATC 3 cut(s) 142, 580, 842
EaeI YGGCCR 1 cut(s) 766
Eco130I CCWWGG 1 cut(s) 121
Eco147I AGGCCT 1 cut(s) 596
Eco47I GGWCC 2 cut(s) 23, 471
Eco88I CYCGRG 1 cut(s) 300
EcoNI CCTNNNNNAGG 1 cut(s) 615
EcoRI GAATTC 2 cut(s) 86, 368
EcoT14I CCWWGG 1 cut(s) 121
EcoT22I ATGCAT 1 cut(s) 667
ErhI CCWWGG 1 cut(s) 121
FaeI CATG 1 cut(s) 478
FatI CATG 1 cut(s) 474
FbaI TGATCA 1 cut(s) 580
Fnu4HI GCNGC 3 cut(s) 354, 702, 705
FokI GGATG 2 cut(s) 704, 756
Fsp4HI GCNGC 3 cut(s) 354, 702, 705
FspBI CTAG 2 cut(s) 194, 870
GlaI GCGC 1 cut(s) 20
GluI GCNGC 3 cut(s) 354, 702, 705
HaeIII GGCC 3 cut(s) 183, 596, 768
HapII CCGG 1 cut(s) 262
HhaI GCGC 1 cut(s) 21
Hin1II CATG 1 cut(s) 478
Hin6I GCGC 1 cut(s) 19
HinP1I GCGC 1 cut(s) 19
HincII GTYRAC 1 cut(s) 329
HindII GTYRAC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 224
HinfI GANTC 8 cut(s) 126, 176, 410, 557, 632, 855, 862, 883
HpaII CCGG 1 cut(s) 262
HphI GGTGA 1 cut(s) 668
Hpy166II GTNNAC 4 cut(s) 268, 329, 685, 934
Hpy188I TCNGA 2 cut(s) 556, 562
Hpy188III TCNNGA 8 cut(s) 77, 302, 449, 539, 644, 773, 840, 859
Hpy8I GTNNAC 4 cut(s) 268, 329, 685, 934
HpyAV CCTTC 2 cut(s) 418, 514
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 964
HpyCH4V TGCA 7 cut(s) 323, 356, 459, 665, 701, 707, 755
HpyF10VI GCNNNNNNNGC 1 cut(s) 320
HpyF3I CTNAG 2 cut(s) 203, 236
HpySE526I ACGT 1 cut(s) 964
Hsp92II CATG 1 cut(s) 478
HspAI GCGC 1 cut(s) 19
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 3 cut(s) 142, 580, 842
LmnI GCTCC 1 cut(s) 780
Lsp1109I GCAGC 3 cut(s) 340, 691, 713
LweI GCATC 1 cut(s) 301
MaeI CTAG 2 cut(s) 194, 870
MaeII ACGT 1 cut(s) 964
MaeIII GTNAC 2 cut(s) 92, 173
MalI GATC 3 cut(s) 144, 582, 844
MboI GATC 3 cut(s) 142, 580, 842
MboII GAAGA 3 cut(s) 16, 76, 857
MhlI GDGCHC 1 cut(s) 38
MlsI TGGCCA 1 cut(s) 768
MluCI AATT 9 cut(s) 7, 68, 86, 287, 368, 515, 756, 912, 938
MluNI TGGCCA 1 cut(s) 768
MlyI GAGTC 2 cut(s) 170, 871
MmeI TCCRAC 2 cut(s) 41, 235
MnlI CCTC 4 cut(s) 421, 478, 618, 638
Mox20I TGGCCA 1 cut(s) 768
Mph1103I ATGCAT 1 cut(s) 667
MroXI GAANNNNTTC 2 cut(s) 8, 68
MscI TGGCCA 1 cut(s) 768
MseI TTAA 2 cut(s) 147, 810
MslI CAYNNNNRTG 1 cut(s) 479
Msp20I TGGCCA 1 cut(s) 768
MspA1I CMGCKG 3 cut(s) 704, 851, 879
MspCI CTTAAG 1 cut(s) 809
MspI CCGG 1 cut(s) 262
MspR9I CCNGG 1 cut(s) 263
MvnI CGCG 2 cut(s) 21, 415
MwoI GCNNNNNNNGC 1 cut(s) 320
NciI CCSGG 1 cut(s) 263
NdeII GATC 3 cut(s) 142, 580, 842
NlaIII CATG 1 cut(s) 478
NlaIV GGNNCC 1 cut(s) 472
NmuCI GTSAC 2 cut(s) 92, 173
NsiI ATGCAT 1 cut(s) 667
PaeR7I CTCGAG 1 cut(s) 300
PceI AGGCCT 1 cut(s) 596
PdmI GAANNNNTTC 2 cut(s) 8, 68
PfeI GAWTC 6 cut(s) 126, 410, 557, 632, 855, 883
PkrI GCNGC 3 cut(s) 355, 703, 706
PleI GAGTC 2 cut(s) 170, 870
PpsI GAGTC 2 cut(s) 170, 870
PspN4I GGNNCC 1 cut(s) 472
PspPI GGNCC 2 cut(s) 23, 471
PstI CTGCAG 2 cut(s) 358, 709
PvuII CAGCTG 3 cut(s) 704, 851, 879
RsaI GTAC 4 cut(s) 133, 192, 686, 835
RsaNI GTAC 4 cut(s) 132, 191, 685, 834
RseI CAYNNNNRTG 1 cut(s) 479
SaqAI TTAA 2 cut(s) 147, 810
SatI GCNGC 3 cut(s) 354, 702, 705
Sau3AI GATC 3 cut(s) 142, 580, 842
Sau96I GGNCC 2 cut(s) 23, 471
ScaI AGTACT 1 cut(s) 192
SchI GAGTC 2 cut(s) 170, 871
ScrFI CCNGG 1 cut(s) 263
SduI GDGCHC 1 cut(s) 38
SfaNI GCATC 1 cut(s) 301
SfcI CTRYAG 3 cut(s) 354, 705, 927
Sfr274I CTCGAG 1 cut(s) 300
SinI GGWCC 2 cut(s) 23, 471
SlaI CTCGAG 1 cut(s) 300
SmiMI CAYNNNNRTG 1 cut(s) 479
SmlI CTYRAG 4 cut(s) 300, 447, 809, 859
SmoI CTYRAG 4 cut(s) 300, 447, 809, 859
Sse9I AATT 9 cut(s) 7, 68, 86, 287, 368, 515, 756, 912, 938
SseBI AGGCCT 1 cut(s) 596
SsiI CCGC 1 cut(s) 21
SspMI CTAG 2 cut(s) 194, 870
StuI AGGCCT 1 cut(s) 596
StyD4I CCNGG 1 cut(s) 261
StyI CCWWGG 1 cut(s) 121
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 967
TaqI TCGA 4 cut(s) 301, 630, 802, 841
TasI AATT 9 cut(s) 7, 68, 86, 287, 368, 515, 756, 912, 938
TatI WGTACW 4 cut(s) 131, 190, 684, 833
TfiI GAWTC 6 cut(s) 126, 410, 557, 632, 855, 883
Tru1I TTAA 2 cut(s) 147, 810
Tru9I TTAA 2 cut(s) 147, 810
TscAI CASTG 2 cut(s) 38, 103
TseFI GTSAC 2 cut(s) 92, 173
TseI GCWGC 3 cut(s) 353, 701, 704
Tsp45I GTSAC 2 cut(s) 92, 173
TspDTI ATGAA 5 cut(s) 17, 62, 99, 120, 750
TspGWI ACGGA 1 cut(s) 469
TspRI CASTG 2 cut(s) 38, 103
Vha464I CTTAAG 1 cut(s) 809
VpaK11BI GGWCC 2 cut(s) 23, 471
XagI CCTNNNNNAGG 1 cut(s) 615
XapI RAATTY 7 cut(s) 7, 68, 86, 287, 368, 912, 938
XhoI CTCGAG 1 cut(s) 300
XmnI GAANNNNTTC 2 cut(s) 8, 68
XspI CTAG 2 cut(s) 194, 870
ZrmI AGTACT 1 cut(s) 192
Zsp2I ATGCAT 1 cut(s) 667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.