Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0014294

Physical Location & Seq

Genomic Coordinates Reverse (-)

1232651 .. 1235166

Gene Structure 2516 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1016200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1017 bp

ATGATTTCATCTTTCTTGTCCATTTGGTACCTTGTATATGAACGGGTGTTCACTGAACTTATTCAGCAGATGCAAGTAAAAGGCGTGCAGGTTGAGGAGCTGTATTCATTGGACCTTGATTCTCTGAACAATCTGAGGCCAGTGTATGGTTTGATTTTCCTATTCAAATGGCGCCCGGGGGAAAAGGATAACCGCCTTGTAATTAAGGATCCAAATCCTAATTTGTTTTTCGCAAGCCAGGTTATTAATAATGCATGTGCAACCCAAGCAATCTTGTCTATCCTGATGAACTGTCCGGATGTTGACATTGGTCCTGAACTGTCGAAACTGAAAGAATTTACAAAGAACTTTCCACCTGAACTGAAAGGATTGGCAATAAACAATAGTGAAGCTATCCGTGCAGCTCATAATAGTTTTGCCAGGCCCGAGCCGTTTGTTTCTGATGAGCAGAAGGTAGCTGACAAAGATGATGACGTCTACCATTTTATAAGCTACTTGCCTGTCGATGGGGTCCTGTATGAGCTTGATGGACTGAAGGAGGGACCCATTAGCCTTGGACAGTGCCCCAGCGGGCAAGGTGACATGGAATGGCTACAGATGGTGCAACCTGTAATCCAGGAACGCATCGAAAGGTATTCCAAAAGTGAGATAAGATTCAATCTCTTGGCCATTATCAAGAACAGGAAAGAGATGTATACTGCTGAGCTCAAGGAACTCCAAAAGAAGAGGGAGCGTATTTTGCAGCAGCTGTCTGCCTTACAGCAAGAGAGAGCGGCCGACGGGGGCAAGTTTGAAGCGCTGAACAAAGCTCTTTCGGAAATCAATGCTGGGATCGAGGGTGCCACCGAGAAGATCTTGATGGAAGAAGAGAAATGTAAGAAGTGGAGAACAGAGAACATTCGCAGGAAGCACAACTACATTCCATTCCTGTTTAACTTCCTCAAGATTCTTGCCGAGAAGAAGCAGTTGAAGCCTCTTATTGAGAAGGCGAAGCAGAAGGCAGGCAACACCAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003824 GO:0004843 GO:0004857 GO:0004866 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009892 GO:0009894 GO:0009987 GO:0010016 GO:0010466 GO:0010605 GO:0010951 GO:0016579 GO:0016787 GO:0019222 GO:0019538 GO:0019783 GO:0030162 GO:0030234 GO:0030414 GO:0031011 GO:0031323 GO:0031324 GO:0031329 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033202 GO:0036211 GO:0036459 GO:0042176 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044877 GO:0045861 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0061136 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070603 GO:0070628 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0097346 GO:0098772 GO:0101005 GO:0140096 GO:1901564 GO:1902494 GO:1903050 GO:1903362 GO:1904949

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_C12

Protein Analysis

339

Amino Acids

38.94

Weight (kDa)

7.59

Isoelectric Point (pI)

42.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_C12	PF01088	15 - 210	1.9e-59	Ubiquitin carboxyl-terminal hydrolase, family 1
UCH_C	PF18031	279 - 323	3.2e-19	Ubiquitin carboxyl-terminal hydrolases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014294)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G65650 AT5G16310
fragaria_vesca	FvH4_4g33330 FvH4_4g33330
malus_domestica	MD13G1018500.v1.1 MD16G1016200.v1.1
prunus_persica	Prupe.1G335400_v2.0.a1 Prupe.1G335400_v2.0.a1
pyrus_communis	pycom13g01570 pycom16g01510
rosa_chinensis	RchiOBHm_Chr4g0442561
rosa_laevigata	RLG00000006002
rosa_multiflora	Rmu_ssc0000204.1_g000045
rosa_roxburghii	Rroxscaffold_5G00383370
rosa_rugosa	Rorug04G0341200
rosa_samantha	Rh4AG393600
rosa_wichuraiana	Rw4G034010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	488
AatII	GACGTC	1 cut(s)	477
Acc36I	ACCTGC	1 cut(s)	79
Acc65I	GGTACC	1 cut(s)	27
AccB1I	GGYRCC	3 cut(s)	27, 171, 839
AccB7I	CCANNNNNTGG	1 cut(s)	146
AccBSI	CCGCTC	1 cut(s)	773
AccI	GTMKAC	2 cut(s)	477, 695
AccIII	TCCGGA	1 cut(s)	295
AciI	CCGC	3 cut(s)	193, 570, 773
AclWI	GGATC	3 cut(s)	203, 216, 839
AcoI	YGGCCR	2 cut(s)	666, 774
AcsI	RAATTY	1 cut(s)	335
AcuI	CTGAAG	1 cut(s)	554
AcyI	GRCGYC	2 cut(s)	172, 474
AfaI	GTAC	1 cut(s)	29
AfeI	AGCGCT	1 cut(s)	798
AfiI	CCNNNNNNNGG	2 cut(s)	146, 506
AgsI	TTSAA	4 cut(s)	166, 658, 794, 970
AjnI	CCWGG	3 cut(s)	237, 419, 615
AluBI	AGCT	9 cut(s)	100, 392, 404, 458, 492, 523, 706, 748, 809
AluI	AGCT	9 cut(s)	100, 392, 404, 458, 492, 523, 706, 748, 809
Alw21I	GWGCWC	1 cut(s)	708
AlwI	GGATC	3 cut(s)	203, 216, 839
AlwNI	CAGNNNCTG	1 cut(s)	748
Ama87I	CYCGRG	2 cut(s)	175, 425
Aor13HI	TCCGGA	1 cut(s)	295
Aor51HI	AGCGCT	1 cut(s)	798
AoxI	GGCC	4 cut(s)	137, 422, 666, 774
ApeKI	GCWGC	3 cut(s)	401, 742, 745
ApoI	RAATTY	1 cut(s)	335
AseI	ATTAAT	1 cut(s)	246
Asp700I	GAANNNNTTC	1 cut(s)	60
Asp718I	GGTACC	1 cut(s)	27
AspLEI	GCGC	2 cut(s)	174, 799
AspS9I	GGNCC	5 cut(s)	112, 311, 423, 511, 542
AsuC2I	CCSGG	2 cut(s)	176, 177
AsuHPI	GGTGA	1 cut(s)	590
AvaI	CYCGRG	2 cut(s)	175, 425
AvaII	GGWCC	4 cut(s)	112, 311, 511, 542
BaeGI	GKGCMC	1 cut(s)	566
BalI	TGGCCA	1 cut(s)	668
BamHI	GGATCC	1 cut(s)	208
BanI	GGYRCC	3 cut(s)	27, 171, 839
BanII	GRGCYC	1 cut(s)	708
BarI	GAAGNNNNNNTAC	2 cut(s)	899, 931
Bbv12I	GWGCWC	1 cut(s)	708
BbvI	GCAGC	3 cut(s)	413, 754, 757
BccI	CCATC	4 cut(s)	500, 521, 592, 853
BceAI	ACGGC	1 cut(s)	415
BciT130I	CCWGG	3 cut(s)	239, 421, 617
BcnI	CCSGG	2 cut(s)	176, 177
BfmI	CTRYAG	1 cut(s)	593
BfoI	RGCGCY	2 cut(s)	175, 800
BfuAI	ACCTGC	1 cut(s)	79
BglII	AGATCT	1 cut(s)	852
BisI	GCNGC	4 cut(s)	402, 743, 746, 774
BlpI	GCTNAGC	1 cut(s)	702
BlsI	GCNGC	4 cut(s)	403, 744, 747, 775
Bme1390I	CCNGG	5 cut(s)	176, 177, 239, 421, 617
Bme18I	GGWCC	4 cut(s)	112, 311, 511, 542
BmeT110I	CYCGRG	2 cut(s)	175, 425
BmgT120I	GGNCC	5 cut(s)	112, 311, 423, 511, 542
BmiI	GGNNCC	7 cut(s)	29, 173, 210, 512, 543, 544, 841
BmrFI	CCNGG	5 cut(s)	176, 177, 239, 421, 617
BmsI	GCATC	2 cut(s)	60, 633
BoxI	GACNNNNGTC	1 cut(s)	309
Bpu1102I	GCTNAGC	1 cut(s)	702
BpuEI	CTTGAG	2 cut(s)	692, 926
BpuMI	CCSGG	2 cut(s)	176, 177
BsaBI	GATNNNNATC	1 cut(s)	213
BsaHI	GRCGYC	2 cut(s)	172, 474
BsaJI	CCNNGG	3 cut(s)	175, 176, 553
BsaWI	WCCGGW	1 cut(s)	295
Bsc4I	CCNNNNNNNGG	2 cut(s)	146, 506
Bse1I	ACTGG	1 cut(s)	140
Bse8I	GATNNNNATC	1 cut(s)	213
BseAI	TCCGGA	1 cut(s)	295
BseBI	CCWGG	3 cut(s)	239, 421, 617
BseDI	CCNNGG	3 cut(s)	175, 176, 553
BseGI	GGATG	1 cut(s)	304
BseJI	GATNNNNATC	1 cut(s)	213
BseLI	CCNNNNNNNGG	2 cut(s)	146, 506
BseMII	CTCAG	2 cut(s)	125, 693
BseNI	ACTGG	1 cut(s)	140
BseRI	GAGGAG	1 cut(s)	110
BseSI	GKGCMC	1 cut(s)	566
BseX3I	CGGCCG	1 cut(s)	774
BseXI	GCAGC	3 cut(s)	413, 754, 757
BseYI	CCCAGC	2 cut(s)	566, 827
BsgI	GTGCAG	2 cut(s)	107, 420
Bsh1285I	CGRYCG	1 cut(s)	777
BshFI	GGCC	4 cut(s)	139, 424, 668, 776
BshNI	GGYRCC	3 cut(s)	27, 171, 839
BsiEI	CGRYCG	1 cut(s)	777
BsiHKAI	GWGCWC	1 cut(s)	708
BsiHKCI	CYCGRG	2 cut(s)	175, 425
BsiSI	CCGG	2 cut(s)	176, 296
BslFI	GGGAC	1 cut(s)	555
BslI	CCNNNNNNNGG	2 cut(s)	146, 506
BsmFI	GGGAC	1 cut(s)	555
BsnI	GGCC	4 cut(s)	139, 424, 668, 776
BsoBI	CYCGRG	2 cut(s)	175, 425
Bsp1286I	GDGCHC	2 cut(s)	566, 708
Bsp13I	TCCGGA	1 cut(s)	295
Bsp143I	GATC	3 cut(s)	208, 831, 852
Bsp1720I	GCTNAGC	1 cut(s)	702
BspACI	CCGC	3 cut(s)	193, 570, 773
BspANI	GGCC	4 cut(s)	139, 424, 668, 776
BspCNI	CTCAG	2 cut(s)	126, 694
BspEI	TCCGGA	1 cut(s)	295
BspLI	GGNNCC	7 cut(s)	29, 173, 210, 512, 543, 544, 841
BspMI	ACCTGC	1 cut(s)	79
BspPI	GGATC	3 cut(s)	203, 216, 839
BspT107I	GGYRCC	3 cut(s)	27, 171, 839
BsrBI	CCGCTC	1 cut(s)	773
BsrI	ACTGG	1 cut(s)	140
BssECI	CCNNGG	3 cut(s)	175, 176, 553
BssMI	GATC	3 cut(s)	208, 831, 852
BssNAI	GTATAC	1 cut(s)	696
BssNI	GRCGYC	2 cut(s)	172, 474
BssT1I	CCWWGG	1 cut(s)	553
Bst1107I	GTATAC	1 cut(s)	696
Bst2UI	CCWGG	3 cut(s)	239, 421, 617
Bst4CI	ACNGT	3 cut(s)	293, 321, 561
Bst6I	CTCTTC	2 cut(s)	719, 861
BstACI	GRCGYC	2 cut(s)	172, 474
BstC8I	GCNNGC	4 cut(s)	86, 235, 572, 1003
BstDEI	CTNAG	2 cut(s)	134, 702
BstF5I	GGATG	1 cut(s)	304
BstH2I	RGCGCY	2 cut(s)	175, 800
BstHHI	GCGC	2 cut(s)	174, 799
BstKTI	GATC	3 cut(s)	211, 834, 855
BstMBI	GATC	3 cut(s)	208, 831, 852
BstMCI	CGRYCG	1 cut(s)	777
BstMWI	GCNNNNNNNGC	4 cut(s)	266, 398, 739, 970
BstNI	CCWGG	3 cut(s)	239, 421, 617
BstNSI	RCATGY	1 cut(s)	258
BstPAI	GACNNNNGTC	1 cut(s)	309
BstSCI	CCNGG	5 cut(s)	174, 175, 237, 419, 615
BstSFI	CTRYAG	1 cut(s)	593
BstSLI	GKGCMC	1 cut(s)	566
BstV1I	GCAGC	3 cut(s)	413, 754, 757
BstX2I	RGATCY	2 cut(s)	208, 852
BstYI	RGATCY	2 cut(s)	208, 852
BstZ17I	GTATAC	1 cut(s)	696
BstZI	CGGCCG	1 cut(s)	774
BsuRI	GGCC	4 cut(s)	139, 424, 668, 776
BtsCI	GGATG	1 cut(s)	304
BtsIMutI	CAGTG	3 cut(s)	51, 147, 566
BveI	ACCTGC	1 cut(s)	79
Cac8I	GCNNGC	4 cut(s)	86, 235, 572, 1003
CaiI	CAGNNNCTG	1 cut(s)	748
CfoI	GCGC	2 cut(s)	174, 799
Cfr13I	GGNCC	5 cut(s)	112, 311, 423, 511, 542
Cfr9I	CCCGGG	1 cut(s)	175
Csp6I	GTAC	1 cut(s)	28
CviAII	CATG	2 cut(s)	255, 583
CviQI	GTAC	1 cut(s)	28
DdeI	CTNAG	2 cut(s)	134, 702
DinI	GGCGCC	1 cut(s)	173
DpnI	GATC	3 cut(s)	210, 833, 854
DpnII	GATC	3 cut(s)	208, 831, 852
EaeI	YGGCCR	2 cut(s)	666, 774
EagI	CGGCCG	1 cut(s)	774
Eam1104I	CTCTTC	2 cut(s)	719, 861
EarI	CTCTTC	2 cut(s)	719, 861
Ecl136II	GAGCTC	1 cut(s)	706
EclXI	CGGCCG	1 cut(s)	774
Eco130I	CCWWGG	1 cut(s)	553
Eco24I	GRGCYC	1 cut(s)	708
Eco47I	GGWCC	4 cut(s)	112, 311, 511, 542
Eco47III	AGCGCT	1 cut(s)	798
Eco52I	CGGCCG	1 cut(s)	774
Eco53kI	GAGCTC	1 cut(s)	706
Eco57I	CTGAAG	1 cut(s)	554
Eco88I	CYCGRG	2 cut(s)	175, 425
EcoICRI	GAGCTC	1 cut(s)	706
EcoO109I	RGGNCCY	2 cut(s)	511, 542
EcoRII	CCWGG	3 cut(s)	237, 419, 615
EcoT14I	CCWWGG	1 cut(s)	553
EcoT22I	ATGCAT	1 cut(s)	256
EcoT38I	GRGCYC	1 cut(s)	708
EgeI	GGCGCC	1 cut(s)	173
EheI	GGCGCC	1 cut(s)	173
ErhI	CCWWGG	1 cut(s)	553
FaeI	CATG	2 cut(s)	258, 586
FaiI	YATR	9 cut(s)	37, 39, 147, 256, 408, 488, 519, 584, 696
FaqI	GGGAC	1 cut(s)	555
FatI	CATG	2 cut(s)	254, 582
FauI	CCCGC	1 cut(s)	563
FblI	GTMKAC	2 cut(s)	477, 695
Fnu4HI	GCNGC	4 cut(s)	402, 743, 746, 774
FokI	GGATG	1 cut(s)	311
FriOI	GRGCYC	1 cut(s)	708
Fsp4HI	GCNGC	4 cut(s)	402, 743, 746, 774
GlaI	GCGC	2 cut(s)	173, 798
GluI	GCNGC	4 cut(s)	402, 743, 746, 774
GsaI	CCCAGC	2 cut(s)	570, 831
HaeII	RGCGCY	2 cut(s)	175, 800
HaeIII	GGCC	4 cut(s)	139, 424, 668, 776
HapII	CCGG	2 cut(s)	176, 296
HhaI	GCGC	2 cut(s)	174, 799
Hin1I	GRCGYC	2 cut(s)	172, 474
Hin1II	CATG	2 cut(s)	258, 586
Hin6I	GCGC	2 cut(s)	172, 797
HinP1I	GCGC	2 cut(s)	172, 797
HincII	GTYRAC	1 cut(s)	304
HindII	GTYRAC	1 cut(s)	304
HinfI	GANTC	3 cut(s)	119, 654, 946
HpaII	CCGG	2 cut(s)	176, 296
HphI	GGTGA	1 cut(s)	590
Hpy166II	GTNNAC	4 cut(s)	51, 304, 478, 696
Hpy188I	TCNGA	4 cut(s)	126, 135, 442, 817
Hpy188III	TCNNGA	6 cut(s)	283, 296, 314, 676, 856, 943
Hpy8I	GTNNAC	4 cut(s)	51, 304, 478, 696
Hpy99I	CGWCG	1 cut(s)	782
HpyAV	CCTTC	4 cut(s)	445, 529, 979, 991
HpyCH4III	ACNGT	3 cut(s)	293, 321, 561
HpyCH4IV	ACGT	1 cut(s)	474
HpyCH4V	TGCA	7 cut(s)	73, 88, 254, 260, 401, 604, 742
HpyF10VI	GCNNNNNNNGC	4 cut(s)	266, 398, 739, 970
HpyF3I	CTNAG	2 cut(s)	134, 702
HpySE526I	ACGT	1 cut(s)	474
Hsp92I	GRCGYC	2 cut(s)	172, 474
Hsp92II	CATG	2 cut(s)	258, 586
HspAI	GCGC	2 cut(s)	172, 797
KasI	GGCGCC	1 cut(s)	171
KflI	GGGWCCC	1 cut(s)	542
Kpn2I	TCCGGA	1 cut(s)	295
KpnI	GGTACC	1 cut(s)	31
Kzo9I	GATC	3 cut(s)	208, 831, 852
LmnI	GCTCC	2 cut(s)	97, 730
Lsp1109I	GCAGC	3 cut(s)	413, 754, 757
LweI	GCATC	2 cut(s)	60, 633
MaeII	ACGT	1 cut(s)	474
MaeIII	GTNAC	1 cut(s)	578
MalI	GATC	3 cut(s)	210, 833, 854
MbiI	CCGCTC	1 cut(s)	773
MboI	GATC	3 cut(s)	208, 831, 852
MboII	GAAGA	5 cut(s)	736, 862, 875, 878, 970
MflI	RGATCY	2 cut(s)	208, 852
MhlI	GDGCHC	2 cut(s)	566, 708
MlsI	TGGCCA	1 cut(s)	668
MluCI	AATT	3 cut(s)	201, 220, 335
MluNI	TGGCCA	1 cut(s)	668
Mly113I	GGCGCC	1 cut(s)	172
MnlI	CCTC	7 cut(s)	88, 129, 532, 720, 829, 950, 984
Mox20I	TGGCCA	1 cut(s)	668
Mph1103I	ATGCAT	1 cut(s)	256
MroI	TCCGGA	1 cut(s)	295
MroXI	GAANNNNTTC	1 cut(s)	60
MscI	TGGCCA	1 cut(s)	668
MseI	TTAA	3 cut(s)	204, 246, 933
Msp20I	TGGCCA	1 cut(s)	668
MspA1I	CMGCKG	2 cut(s)	570, 748
MspI	CCGG	2 cut(s)	176, 296
MspR9I	CCNGG	5 cut(s)	176, 177, 239, 421, 617
MvaI	CCWGG	3 cut(s)	239, 421, 617
MwoI	GCNNNNNNNGC	4 cut(s)	266, 398, 739, 970
NarI	GGCGCC	1 cut(s)	172
NciI	CCSGG	2 cut(s)	176, 177
NdeII	GATC	3 cut(s)	208, 831, 852
NlaIII	CATG	2 cut(s)	258, 586
NlaIV	GGNNCC	7 cut(s)	29, 173, 210, 512, 543, 544, 841
NmeAIII	GCCGAG	1 cut(s)	979
NmuCI	GTSAC	1 cut(s)	578
NsiI	ATGCAT	1 cut(s)	256
NspI	RCATGY	1 cut(s)	258
PdmI	GAANNNNTTC	1 cut(s)	60
PfeI	GAWTC	3 cut(s)	119, 654, 946
PflMI	CCANNNNNTGG	1 cut(s)	146
PfoI	TCCNGGA	1 cut(s)	615
PkrI	GCNGC	4 cut(s)	403, 744, 747, 775
PluTI	GGCGCC	1 cut(s)	175
PpuMI	RGGWCCY	2 cut(s)	511, 542
PshAI	GACNNNNGTC	1 cut(s)	309
PshBI	ATTAAT	1 cut(s)	246
PsiI	TTATAA	1 cut(s)	488
Psp124BI	GAGCTC	1 cut(s)	708
Psp5II	RGGWCCY	2 cut(s)	511, 542
Psp6I	CCWGG	3 cut(s)	237, 419, 615
PspFI	CCCAGC	2 cut(s)	566, 827
PspGI	CCWGG	3 cut(s)	237, 419, 615
PspN4I	GGNNCC	7 cut(s)	29, 173, 210, 512, 543, 544, 841
PspPI	GGNCC	5 cut(s)	112, 311, 423, 511, 542
PspPPI	RGGWCCY	2 cut(s)	511, 542
PstNI	CAGNNNCTG	1 cut(s)	748
PsuI	RGATCY	2 cut(s)	208, 852
PvuII	CAGCTG	1 cut(s)	748
RsaI	GTAC	1 cut(s)	29
RsaNI	GTAC	1 cut(s)	28
SacI	GAGCTC	1 cut(s)	708
SaqAI	TTAA	3 cut(s)	204, 246, 933
SatI	GCNGC	4 cut(s)	402, 743, 746, 774
Sau3AI	GATC	3 cut(s)	208, 831, 852
Sau96I	GGNCC	5 cut(s)	112, 311, 423, 511, 542
ScrFI	CCNGG	5 cut(s)	176, 177, 239, 421, 617
SduI	GDGCHC	2 cut(s)	566, 708
SfaNI	GCATC	2 cut(s)	60, 633
SfcI	CTRYAG	1 cut(s)	593
SfoI	GGCGCC	1 cut(s)	173
SinI	GGWCC	4 cut(s)	112, 311, 511, 542
SmaI	CCCGGG	1 cut(s)	177
SmlI	CTYRAG	2 cut(s)	707, 941
SmoI	CTYRAG	2 cut(s)	707, 941
Sse9I	AATT	3 cut(s)	201, 220, 335
SsiI	CCGC	3 cut(s)	193, 570, 773
SspDI	GGCGCC	1 cut(s)	171
SstI	GAGCTC	1 cut(s)	708
StyD4I	CCNGG	5 cut(s)	174, 175, 237, 419, 615
StyI	CCWWGG	1 cut(s)	553
TaaI	ACNGT	3 cut(s)	293, 321, 561
TaiI	ACGT	1 cut(s)	477
TaqI	TCGA	4 cut(s)	323, 504, 627, 834
TasI	AATT	3 cut(s)	201, 220, 335
TauI	GCSGC	1 cut(s)	776
TfiI	GAWTC	3 cut(s)	119, 654, 946
Tru1I	TTAA	3 cut(s)	204, 246, 933
Tru9I	TTAA	3 cut(s)	204, 246, 933
TscAI	CASTG	3 cut(s)	58, 147, 566
TseFI	GTSAC	1 cut(s)	578
TseI	GCWGC	3 cut(s)	401, 742, 745
Tsp45I	GTSAC	1 cut(s)	578
TspDTI	ATGAA	3 cut(s)	54, 96, 302
TspGWI	ACGGA	1 cut(s)	386
TspMI	CCCGGG	1 cut(s)	175
TspRI	CASTG	3 cut(s)	58, 147, 566
Van91I	CCANNNNNTGG	1 cut(s)	146
VpaK11BI	GGWCC	4 cut(s)	112, 311, 511, 542
VspI	ATTAAT	1 cut(s)	246
XapI	RAATTY	1 cut(s)	335
XceI	RCATGY	1 cut(s)	258
XmaI	CCCGGG	1 cut(s)	175
XmiI	GTMKAC	2 cut(s)	477, 695
XmnI	GAANNNNTTC	1 cut(s)	60
ZraI	GACGTC	1 cut(s)	475
Zsp2I	ATGCAT	1 cut(s)	256

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.