Prupe.1G335400_v2.0.a1

Ubiquitin carboxyl-terminal hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0014294
Physical Location & Seq
Forward (+)
31679422 .. 31682411
2990 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G335400.1

Sequence Viewer

Length: 1005 bp
ATGTCTTGGTGCACGATTGAGTCCGACCCAGGGGTGTTTACAGAACTTATTCAGCAGATGCAAGTAAAAGGCGTGCAGGTTGAGGAGTTGTATTCATTGGACCTTGATTCTCTGAACAATCTGAGGCCGGTGTATGGTTTGATTTTCCTATTCAAATGGCGTCCAGGGGAAAGGGATGATCGCCTTGTAATTAAGGATCCAAATCCTAATTTGTTTTTTGCAAGCCAGGTTATTAATAATGCTTGTGCCACCCAAGCAATCTTGTCTATCCTCATGAACTGTCCAGATGTTGACATTGGTCCTGAACTGTCAAAACTGAAAGAGTTTACAAAGAATTTTCCACCTGAACTGAAAGGGCTGGCAATAAATAACAGCGACGCTATCCGTGCAGCCCATAATAGTTTTGCAAGGCCTGAGCCGTTTATTCCTGAGGAGCAGAAGGTAGCTGAAAAAGATGACGATGTCTACCATTTTATAAGCTACTTGCCTGTTGATGGGGTCCTGTATGAGCTTGATGGATTGAAGGAGGGGCCGATTAGCCTTGGACAGTGCCCTGGTGGGCAAGGTGACATGGAATGGCTGCAGATGGTACAACCTGTAATCCAGGAGCGCATTGAAAGGTATTCCAAAAGTGAGATAAGATTCAATCTCTTGGCCATAATAAAGAACAGGAAAGAGATGTATACTGCTGAACTTAAGGAACTCCAAAAGAAGAGGGAGCGTATTTTGCAGCAGCTGTCTGCCTTACAATCAGAGAGACCGGCCGACAGTAGCAAATTTGAAGCGCTGAACAAAGGTCTTTCAGAAATTAATGCTGGGATCGAGGGCGCTACCGAGAAGATCTTGATGGAAGAAGAAAAATTTAAGAAGTGGAGGACAGAAAATATTCGCCGGAAGCACAACTACATACCCTTTTTGTTTAACTTCCTCAAGATTCTTGCTGAGAAGAAGCAGCTGAAGCCTCTTATTGAGAAGGCAAAGCAAAAATCTGGCAACCCTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000228 GO:0000785 GO:0000790 GO:0003674 GO:0003824 GO:0004843 GO:0004857 GO:0004866 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005694 GO:0005737 GO:0005739 GO:0005829 GO:0006464 GO:0006508 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009653 GO:0009892 GO:0009894 GO:0009987 GO:0010016 GO:0010466 GO:0010605 GO:0010951 GO:0016579 GO:0016787 GO:0019222 GO:0019538 GO:0019783 GO:0030162 GO:0030234 GO:0030414 GO:0031011 GO:0031323 GO:0031324 GO:0031329 GO:0031974 GO:0031981 GO:0032268 GO:0032269 GO:0032501 GO:0032502 GO:0032991 GO:0033202 GO:0036211 GO:0036459 GO:0042176 GO:0043086 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0043412 GO:0044092 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044427 GO:0044428 GO:0044444 GO:0044446 GO:0044454 GO:0044464 GO:0044877 GO:0045861 GO:0048367 GO:0048519 GO:0048523 GO:0048731 GO:0048856 GO:0050789 GO:0050790 GO:0050794 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051336 GO:0051346 GO:0052547 GO:0052548 GO:0060255 GO:0061134 GO:0061135 GO:0061136 GO:0065007 GO:0065009 GO:0070011 GO:0070013 GO:0070603 GO:0070628 GO:0070646 GO:0070647 GO:0071704 GO:0080090 GO:0097346 GO:0098772 GO:0101005 GO:0140096 GO:1901564 GO:1902494 GO:1903050 GO:1903362 GO:1904949
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_C12

Protein Analysis

335

Amino Acids

38.38

Weight (kDa)

6.11

Isoelectric Point (pI)

49.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G65650 AT5G16310
fragaria_vesca FvH4_4g33330 FvH4_4g33330
malus_domestica MD13G1018500.v1.1 MD16G1016200.v1.1
prunus_persica Prupe.1G335400_v2.0.a1 Prupe.1G335400_v2.0.a1
pyrus_communis pycom13g01570 pycom16g01510
rosa_chinensis RchiOBHm_Chr4g0442561
rosa_laevigata RLG00000006002
rosa_multiflora Rmu_ssc0000204.1_g000045
rosa_roxburghii Rroxscaffold_5G00383370
rosa_rugosa Rorug04G0341200
rosa_samantha Rh4AG393600
rosa_wichuraiana Rw4G034010

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 476
Acc36I ACCTGC 1 cut(s) 67
AccI GTMKAC 2 cut(s) 465, 683
AclWI GGATC 3 cut(s) 191, 204, 827
AcoI YGGCCR 2 cut(s) 654, 762
AcsI RAATTY 3 cut(s) 334, 776, 860
AcuI CTGAAG 1 cut(s) 977
AcyI GRCGYC 1 cut(s) 160
AfaI GTAC 1 cut(s) 591
AfeI AGCGCT 1 cut(s) 786
AfiI CCNNNNNNNGG 3 cut(s) 30, 134, 494
AflII CTTAAG 1 cut(s) 695
AgsI TTSAA 5 cut(s) 154, 523, 617, 646, 782
AjnI CCWGG 5 cut(s) 28, 163, 225, 553, 603
AluBI AGCT 5 cut(s) 446, 480, 511, 736, 955
AluI AGCT 5 cut(s) 446, 480, 511, 736, 955
Alw21I GWGCWC 1 cut(s) 14
Alw26I GTCTC 1 cut(s) 751
Alw44I GTGCAC 1 cut(s) 10
AlwI GGATC 3 cut(s) 191, 204, 827
AlwNI CAGNNNCTG 1 cut(s) 736
Aor51HI AGCGCT 1 cut(s) 786
AoxI GGCC 5 cut(s) 125, 410, 530, 654, 762
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 5 cut(s) 389, 580, 730, 733, 952
ApoI RAATTY 3 cut(s) 334, 776, 860
AseI ATTAAT 2 cut(s) 234, 810
Asp700I GAANNNNTTC 2 cut(s) 48, 885
AspLEI GCGC 3 cut(s) 612, 787, 830
AspS9I GGNCC 4 cut(s) 100, 299, 499, 530
AsuHPI GGTGA 1 cut(s) 578
AvaII GGWCC 3 cut(s) 100, 299, 499
AxyI CCTNAGG 1 cut(s) 429
BaeGI GKGCMC 2 cut(s) 14, 554
BalI TGGCCA 1 cut(s) 656
BamHI GGATCC 1 cut(s) 196
BarI GAAGNNNNNNTAC 2 cut(s) 887, 919
Bbv12I GWGCWC 1 cut(s) 14
BbvI GCAGC 5 cut(s) 401, 567, 742, 745, 964
BccI CCATC 4 cut(s) 488, 509, 580, 841
BceAI ACGGC 1 cut(s) 403
BciT130I CCWGG 5 cut(s) 30, 165, 227, 555, 605
BcoDI GTCTC 1 cut(s) 751
BfaI CTAG 1 cut(s) 999
BfmI CTRYAG 1 cut(s) 581
BfoI RGCGCY 2 cut(s) 788, 831
BfrI CTTAAG 1 cut(s) 695
BfuAI ACCTGC 1 cut(s) 67
BglII AGATCT 1 cut(s) 840
BisI GCNGC 5 cut(s) 390, 581, 731, 734, 953
BlsI GCNGC 5 cut(s) 391, 582, 732, 735, 954
Bme1390I CCNGG 5 cut(s) 30, 165, 227, 555, 605
Bme18I GGWCC 3 cut(s) 100, 299, 499
BmgT120I GGNCC 4 cut(s) 100, 299, 499, 530
BmiI GGNNCC 3 cut(s) 198, 500, 531
BmrFI CCNGG 5 cut(s) 30, 165, 227, 555, 605
BmsI GCATC 1 cut(s) 48
BoxI GACNNNNGTC 1 cut(s) 297
Bpu10I CCTNAGC 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 914
BsaBI GATNNNNATC 1 cut(s) 201
BsaHI GRCGYC 1 cut(s) 160
BsaI GGTCTC 1 cut(s) 751
BsaJI CCNNGG 5 cut(s) 28, 29, 164, 541, 553
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 134, 494
Bse118I RCCGGY 2 cut(s) 127, 760
Bse21I CCTNAGG 1 cut(s) 429
Bse8I GATNNNNATC 1 cut(s) 201
BseBI CCWGG 5 cut(s) 30, 165, 227, 555, 605
BseDI CCNNGG 5 cut(s) 28, 29, 164, 541, 553
BseGI GGATG 1 cut(s) 181
BseJI GATNNNNATC 1 cut(s) 201
BseLI CCNNNNNNNGG 3 cut(s) 30, 134, 494
BseMII CTCAG 4 cut(s) 113, 405, 420, 933
BseRI GAGGAG 2 cut(s) 98, 446
BseSI GKGCMC 2 cut(s) 14, 554
BseX3I CGGCCG 1 cut(s) 762
BseXI GCAGC 5 cut(s) 401, 567, 742, 745, 964
BseYI CCCAGC 1 cut(s) 815
BsgI GTGCAG 2 cut(s) 95, 408
Bsh1285I CGRYCG 1 cut(s) 765
BshFI GGCC 5 cut(s) 127, 412, 532, 656, 764
BsiEI CGRYCG 1 cut(s) 765
BsiHKAI GWGCWC 1 cut(s) 14
BsiSI CCGG 3 cut(s) 128, 761, 892
BslI CCNNNNNNNGG 3 cut(s) 30, 134, 494
BsmAI GTCTC 1 cut(s) 751
BsnI GGCC 5 cut(s) 127, 412, 532, 656, 764
Bso31I GGTCTC 1 cut(s) 751
Bsp1286I GDGCHC 2 cut(s) 14, 554
Bsp143I GATC 4 cut(s) 178, 196, 819, 840
BspANI GGCC 5 cut(s) 127, 412, 532, 656, 764
BspCNI CTCAG 4 cut(s) 114, 406, 421, 934
BspHI TCATGA 1 cut(s) 273
BspLI GGNNCC 3 cut(s) 198, 500, 531
BspMAI CTGCAG 1 cut(s) 585
BspMI ACCTGC 1 cut(s) 67
BspPI GGATC 3 cut(s) 191, 204, 827
BspTI CTTAAG 1 cut(s) 695
BspTNI GGTCTC 1 cut(s) 751
BsrFI RCCGGY 2 cut(s) 127, 760
BssAI RCCGGY 2 cut(s) 127, 760
BssECI CCNNGG 5 cut(s) 28, 29, 164, 541, 553
BssMI GATC 4 cut(s) 178, 196, 819, 840
BssNAI GTATAC 1 cut(s) 684
BssNI GRCGYC 1 cut(s) 160
BssT1I CCWWGG 1 cut(s) 541
Bst1107I GTATAC 1 cut(s) 684
Bst2UI CCWGG 5 cut(s) 30, 165, 227, 555, 605
Bst4CI ACNGT 4 cut(s) 281, 309, 549, 770
Bst6I CTCTTC 1 cut(s) 707
BstACI GRCGYC 1 cut(s) 160
BstAFI CTTAAG 1 cut(s) 695
BstC8I GCNNGC 3 cut(s) 74, 223, 360
BstDEI CTNAG 4 cut(s) 122, 414, 429, 942
BstF5I GGATG 1 cut(s) 181
BstH2I RGCGCY 2 cut(s) 788, 831
BstHHI GCGC 3 cut(s) 612, 787, 830
BstKTI GATC 4 cut(s) 181, 199, 822, 843
BstMAI GTCTC 1 cut(s) 751
BstMBI GATC 4 cut(s) 178, 196, 819, 840
BstMCI CGRYCG 1 cut(s) 765
BstMWI GCNNNNNNNGC 4 cut(s) 254, 386, 727, 958
BstNI CCWGG 5 cut(s) 30, 165, 227, 555, 605
BstPAI GACNNNNGTC 1 cut(s) 297
BstSCI CCNGG 5 cut(s) 28, 163, 225, 553, 603
BstSFI CTRYAG 1 cut(s) 581
BstSLI GKGCMC 2 cut(s) 14, 554
BstV1I GCAGC 5 cut(s) 401, 567, 742, 745, 964
BstX2I RGATCY 2 cut(s) 196, 840
BstYI RGATCY 2 cut(s) 196, 840
BstZ17I GTATAC 1 cut(s) 684
BstZI CGGCCG 1 cut(s) 762
Bsu36I CCTNAGG 1 cut(s) 429
BsuRI GGCC 5 cut(s) 127, 412, 532, 656, 764
BtsCI GGATG 1 cut(s) 181
BtsIMutI CAGTG 1 cut(s) 554
BveI ACCTGC 1 cut(s) 67
Cac8I GCNNGC 3 cut(s) 74, 223, 360
CaiI CAGNNNCTG 1 cut(s) 736
CciI TCATGA 1 cut(s) 273
CfoI GCGC 3 cut(s) 612, 787, 830
Cfr10I RCCGGY 2 cut(s) 127, 760
Cfr13I GGNCC 4 cut(s) 100, 299, 499, 530
CseI GACGC 2 cut(s) 149, 386
Csp6I GTAC 1 cut(s) 590
CviAII CATG 2 cut(s) 274, 571
CviQI GTAC 1 cut(s) 590
DdeI CTNAG 4 cut(s) 122, 414, 429, 942
DpnI GATC 4 cut(s) 180, 198, 821, 842
DpnII GATC 4 cut(s) 178, 196, 819, 840
EaeI YGGCCR 2 cut(s) 654, 762
EagI CGGCCG 1 cut(s) 762
Eam1104I CTCTTC 1 cut(s) 707
EarI CTCTTC 1 cut(s) 707
EclXI CGGCCG 1 cut(s) 762
Eco130I CCWWGG 1 cut(s) 541
Eco147I AGGCCT 1 cut(s) 412
Eco31I GGTCTC 1 cut(s) 751
Eco47I GGWCC 3 cut(s) 100, 299, 499
Eco47III AGCGCT 1 cut(s) 786
Eco52I CGGCCG 1 cut(s) 762
Eco57I CTGAAG 1 cut(s) 977
Eco81I CCTNAGG 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 499
EcoRII CCWGG 5 cut(s) 28, 163, 225, 553, 603
EcoT14I CCWWGG 1 cut(s) 541
ErhI CCWWGG 1 cut(s) 541
FaeI CATG 2 cut(s) 277, 574
FaiI YATR 9 cut(s) 135, 275, 396, 476, 507, 572, 659, 684, 908
FatI CATG 2 cut(s) 273, 570
FblI GTMKAC 2 cut(s) 465, 683
Fnu4HI GCNGC 5 cut(s) 390, 581, 731, 734, 953
FokI GGATG 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 390, 581, 731, 734, 953
FspBI CTAG 1 cut(s) 999
GlaI GCGC 3 cut(s) 611, 786, 829
GluI GCNGC 5 cut(s) 390, 581, 731, 734, 953
GsaI CCCAGC 1 cut(s) 819
HaeII RGCGCY 2 cut(s) 788, 831
HaeIII GGCC 5 cut(s) 127, 412, 532, 656, 764
HapII CCGG 3 cut(s) 128, 761, 892
HgaI GACGC 2 cut(s) 149, 386
HhaI GCGC 3 cut(s) 612, 787, 830
Hin1I GRCGYC 1 cut(s) 160
Hin1II CATG 2 cut(s) 277, 574
Hin6I GCGC 3 cut(s) 610, 785, 828
HinP1I GCGC 3 cut(s) 610, 785, 828
HincII GTYRAC 1 cut(s) 292
HindII GTYRAC 1 cut(s) 292
HinfI GANTC 4 cut(s) 20, 107, 642, 934
HpaII CCGG 3 cut(s) 128, 761, 892
HphI GGTGA 1 cut(s) 578
Hpy166II GTNNAC 6 cut(s) 12, 39, 292, 327, 466, 684
Hpy188I TCNGA 5 cut(s) 25, 114, 123, 754, 805
Hpy188III TCNNGA 6 cut(s) 274, 284, 302, 428, 844, 931
Hpy8I GTNNAC 6 cut(s) 12, 39, 292, 327, 466, 684
Hpy99I CGWCG 1 cut(s) 380
HpyAV CCTTC 3 cut(s) 433, 517, 967
HpyCH4III ACNGT 4 cut(s) 281, 309, 549, 770
HpyCH4V TGCA 8 cut(s) 12, 61, 76, 221, 389, 407, 583, 730
HpyF10VI GCNNNNNNNGC 4 cut(s) 254, 386, 727, 958
HpyF3I CTNAG 4 cut(s) 122, 414, 429, 942
Hsp92I GRCGYC 1 cut(s) 160
Hsp92II CATG 2 cut(s) 277, 574
HspAI GCGC 3 cut(s) 610, 785, 828
Kzo9I GATC 4 cut(s) 178, 196, 819, 840
LmnI GCTCC 3 cut(s) 433, 607, 718
Lsp1109I GCAGC 5 cut(s) 401, 567, 742, 745, 964
LweI GCATC 1 cut(s) 48
MaeI CTAG 1 cut(s) 999
MaeIII GTNAC 1 cut(s) 566
MalI GATC 4 cut(s) 180, 198, 821, 842
MboI GATC 4 cut(s) 178, 196, 819, 840
MboII GAAGA 5 cut(s) 724, 850, 863, 866, 958
MflI RGATCY 2 cut(s) 196, 840
MhlI GDGCHC 2 cut(s) 14, 554
MlsI TGGCCA 1 cut(s) 656
MluCI AATT 6 cut(s) 189, 208, 334, 776, 807, 860
MluNI TGGCCA 1 cut(s) 656
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 48
Mox20I TGGCCA 1 cut(s) 656
MroXI GAANNNNTTC 2 cut(s) 48, 885
MscI TGGCCA 1 cut(s) 656
MseI TTAA 6 cut(s) 192, 234, 696, 810, 864, 921
Msp20I TGGCCA 1 cut(s) 656
MspA1I CMGCKG 2 cut(s) 736, 955
MspCI CTTAAG 1 cut(s) 695
MspI CCGG 3 cut(s) 128, 761, 892
MspR9I CCNGG 5 cut(s) 30, 165, 227, 555, 605
MvaI CCWGG 5 cut(s) 30, 165, 227, 555, 605
MwoI GCNNNNNNNGC 4 cut(s) 254, 386, 727, 958
NdeII GATC 4 cut(s) 178, 196, 819, 840
NlaIII CATG 2 cut(s) 277, 574
NlaIV GGNNCC 3 cut(s) 198, 500, 531
NmuCI GTSAC 1 cut(s) 566
PagI TCATGA 1 cut(s) 273
PasI CCCWGGG 1 cut(s) 29
PceI AGGCCT 1 cut(s) 412
PdmI GAANNNNTTC 2 cut(s) 48, 885
PfeI GAWTC 3 cut(s) 107, 642, 934
PflFI GACNNNGTC 1 cut(s) 461
PfoI TCCNGGA 1 cut(s) 603
PkrI GCNGC 5 cut(s) 391, 582, 732, 735, 954
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
PpuMI RGGWCCY 1 cut(s) 499
PshAI GACNNNNGTC 1 cut(s) 297
PshBI ATTAAT 2 cut(s) 234, 810
PsiI TTATAA 1 cut(s) 476
Psp5II RGGWCCY 1 cut(s) 499
Psp6I CCWGG 5 cut(s) 28, 163, 225, 553, 603
PspFI CCCAGC 1 cut(s) 815
PspGI CCWGG 5 cut(s) 28, 163, 225, 553, 603
PspN4I GGNNCC 3 cut(s) 198, 500, 531
PspPI GGNCC 4 cut(s) 100, 299, 499, 530
PspPPI RGGWCCY 1 cut(s) 499
PstI CTGCAG 1 cut(s) 585
PstNI CAGNNNCTG 1 cut(s) 736
PsuI RGATCY 2 cut(s) 196, 840
PsyI GACNNNGTC 1 cut(s) 461
PvuII CAGCTG 2 cut(s) 736, 955
RsaI GTAC 1 cut(s) 591
RsaNI GTAC 1 cut(s) 590
SaqAI TTAA 6 cut(s) 192, 234, 696, 810, 864, 921
SatI GCNGC 5 cut(s) 390, 581, 731, 734, 953
Sau3AI GATC 4 cut(s) 178, 196, 819, 840
Sau96I GGNCC 4 cut(s) 100, 299, 499, 530
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 5 cut(s) 30, 165, 227, 555, 605
SduI GDGCHC 2 cut(s) 14, 554
SfaNI GCATC 1 cut(s) 48
SfcI CTRYAG 1 cut(s) 581
SinI GGWCC 3 cut(s) 100, 299, 499
SmlI CTYRAG 2 cut(s) 695, 929
SmoI CTYRAG 2 cut(s) 695, 929
Sse9I AATT 6 cut(s) 189, 208, 334, 776, 807, 860
SseBI AGGCCT 1 cut(s) 412
SspI AATATT 1 cut(s) 886
SspMI CTAG 1 cut(s) 999
StuI AGGCCT 1 cut(s) 412
StyD4I CCNGG 5 cut(s) 28, 163, 225, 553, 603
StyI CCWWGG 1 cut(s) 541
TaaI ACNGT 4 cut(s) 281, 309, 549, 770
TaqI TCGA 1 cut(s) 822
TasI AATT 6 cut(s) 189, 208, 334, 776, 807, 860
TfiI GAWTC 3 cut(s) 107, 642, 934
Tru1I TTAA 6 cut(s) 192, 234, 696, 810, 864, 921
Tru9I TTAA 6 cut(s) 192, 234, 696, 810, 864, 921
TscAI CASTG 1 cut(s) 554
TseFI GTSAC 1 cut(s) 566
TseI GCWGC 5 cut(s) 389, 580, 730, 733, 952
Tsp45I GTSAC 1 cut(s) 566
TspDTI ATGAA 2 cut(s) 84, 290
TspGWI ACGGA 1 cut(s) 374
TspRI CASTG 1 cut(s) 554
Tth111I GACNNNGTC 1 cut(s) 461
Vha464I CTTAAG 1 cut(s) 695
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 3 cut(s) 100, 299, 499
VspI ATTAAT 2 cut(s) 234, 810
XapI RAATTY 3 cut(s) 334, 776, 860
XmiI GTMKAC 2 cut(s) 465, 683
XmnI GAANNNNTTC 2 cut(s) 48, 885
XspI CTAG 1 cut(s) 999
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.