Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0008391

Physical Location & Seq

Genomic Coordinates Reverse (-)

24789735 .. 24792163

Gene Structure 2429 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G66460.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1404 bp

ATGATTCTTGATTTGGGTTTTCCTTGTTTTGTTCCTCCTCGAACCAGCTCTCGTGAGGACAACAAAGCTTGGCTTCTGGCTGAAACAGAGCCGAAGCTTATTGACTCAGAACAACATTCGTTGCAGTCTTCGTTTAGGTTTAGTCTTTGCTCACAGTTGGAGCTGGAGAAGATTAAAAAGGAGAAACCTTCGTTGTCTTATCGGAATTTTCCAGTGTCTGAAGGATCAGAGACGGTTCTGCTAGTGAATCTGGAGAATGAGACAGGAGAATTGACAGGTGAGATGAATTGGTCGAGAGGCCTTTCACTGGAGAAGAGTATTTCTCCGGTGGCCGATTCTTTGATCCGATTCAGTTACCGCGAACTCCTCACTGCCACGCGCAATTTCTCAAAACGGAGGGTTTTGGGAAGAGGAGCTTGTAGCTATGTTTTTAAGGGAAGAATCGGGATTTGGCGTAAAGCCGTGGCCATCAAAAGACTTGATAAAAAAGATAAAGAATCTCCAAAGTCGTTTTGCAGAGAGTTGATGATTGCAAGCTCTCTTAATAGCCCCAACGTTGTGCCTCTGCTAGGTTTCTGTATCGATCCCGATCAAGGGCTTTTCTTGGTGTACAAGTATGTGTCTGGTGGCAGCCTCGAACGCTTTTTACATGATAAGAAGAAAAAGAAGAGTAGGAAGACCCCCTTGAATCTGCCTTGGTCTACAAGGTACAAGGTTGCCTTAGGTATTGCAGATGCCATAGCCTATTTACATAATGGCACTGAGCAATGCGTTGTGCATAGAGACATTAAACCCTCAAATATTCTTCTTTCCTCAAACAAAATTCCAAAGTTGTGTGATTTTGGGTTGGCTACTTGGACCGCTGCGCCTTCGGTTCCTTTCCTCTGTAAAACCGTGAAAGGAACTTTTGGTTATCTGGCTCCTGAGTATTTCCAACACGGCAAGATATCTGACAAGACCGATGTTTACGCATTTGGAGTCGTGTTGCTTGAGCTAATAACTGGTCGGAAGCCAATTGAAGCAAGAAGACCATCTGGTGAAGAAAATTTGGTAGTTTGGGCAAAACCGTTGTTGCATAGAGGGATAGAAGCTACAGAGGAGTTGCTAGATCCAAGGCTGAAATGTACTAGAAAAAACTCGGCTTCGATGGAGCGTATGATCCGAGCTGCGGCAGCGTGTGTGATCAATGAGGAATCACGAAGACCGGGGATGAAGGAGATACTTTCAATCCTAAAAGGCGGTGAAGGGATAGAACTAAGGACGTTATCAAGCCGGAAGAAATCAAATCTTCCGGGTATAATGGACTGTTATCCGCAGTTGCAACGGACAAAATCTGAGATGAAGAGTCATCTTACGCTTGCGATGCTCGGAGTAACGGAATTTGAAGCTGATGATCTTTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

467

Amino Acids

52.49

Weight (kDa)

9.35

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	157 - 333	2.9e-30	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	159 - 312	1.1e-27	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008391)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66460 AT1G66460 AT1G66460 AT5G37790
fragaria_vesca	FvH4_1g28910 FvH4_1g28910
malus_domestica	MD09G1262600.v1.1 MD17G1257500.v1.1
prunus_persica	Prupe.3G160300_v2.0.a1 Prupe.3G160300_v2.0.a1 Prupe.3G160300_v2.0.a1
pyrus_communis	pycom09g17690 pycom17g25960
rosa_chinensis	RchiOBHm_Chr3g0493411
rosa_laevigata	RLG00000022715
rosa_multiflora	Rmu_sc0000828.1_g000022
rosa_roxburghii	Rroxscaffold_6G00391600
rosa_rugosa	Rorug03G0260900
rosa_samantha	Rh3AG310000 Rh3BG346100 Rh3CG343500 Rh3DG345100
rosa_wichuraiana	Rw3G027230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccI	GTMKAC	1 cut(s)	701
AccII	CGCG	2 cut(s)	360, 379
AciI	CCGC	5 cut(s)	358, 861, 1169, 1239, 1313
AclI	AACGTT	1 cut(s)	555
AclWI	GGATC	5 cut(s)	232, 337, 578, 1103, 1153
AcoI	YGGCCR	2 cut(s)	330, 465
AcsI	RAATTY	4 cut(s)	205, 822, 1045, 1379
AcuI	CTGAAG	1 cut(s)	240
AfaI	GTAC	3 cut(s)	611, 710, 1126
AfiI	CCNNNNNNNGG	5 cut(s)	307, 569, 593, 594, 1168
AgsI	TTSAA	4 cut(s)	688, 1019, 1227, 1385
AjuI	GAANNNNNNNTTGG	2 cut(s)	433, 465
Alw26I	GTCTC	3 cut(s)	224, 254, 777
AlwI	GGATC	5 cut(s)	232, 337, 578, 1103, 1153
AlwNI	CAGNNNCTG	1 cut(s)	218
AoxI	GGCC	3 cut(s)	298, 330, 465
ApeKI	GCWGC	4 cut(s)	630, 863, 1166, 1172
ApoI	RAATTY	4 cut(s)	205, 822, 1045, 1379
AspLEI	GCGC	2 cut(s)	381, 868
AspS9I	GGNCC	1 cut(s)	858
AsuC2I	CCSGG	2 cut(s)	1206, 1293
AsuHPI	GGTGA	3 cut(s)	290, 1049, 1253
AvaII	GGWCC	1 cut(s)	858
AxyI	CCTNAGG	1 cut(s)	721
BalI	TGGCCA	1 cut(s)	467
BauI	CACGAG	1 cut(s)	51
BbsI	GAAGAC	4 cut(s)	120, 683, 1033, 1207
BbvI	GCAGC	4 cut(s)	642, 850, 1153, 1184
BccI	CCATC	3 cut(s)	476, 1039, 1141
BceAI	ACGGC	2 cut(s)	446, 955
BclI	TGATCA	1 cut(s)	1182
BcnI	CCSGG	2 cut(s)	1206, 1293
BcoDI	GTCTC	3 cut(s)	224, 254, 777
BfaI	CTAG	4 cut(s)	242, 569, 1106, 1128
BfmI	CTRYAG	1 cut(s)	1092
BisI	GCNGC	5 cut(s)	631, 864, 1167, 1170, 1173
BlsI	GCNGC	5 cut(s)	632, 865, 1168, 1171, 1174
Bme1390I	CCNGG	2 cut(s)	1206, 1293
Bme18I	GGWCC	1 cut(s)	858
BmgT120I	GGNCC	1 cut(s)	858
BmiI	GGNNCC	2 cut(s)	876, 921
BmrFI	CCNGG	2 cut(s)	1206, 1293
BmsI	GCATC	2 cut(s)	724, 1353
BpiI	GAAGAC	4 cut(s)	120, 683, 1033, 1207
BplI	GAGNNNNNCTC	2 cut(s)	307, 339
BpmI	CTGGAG	3 cut(s)	185, 272, 329
BpuEI	CTTGAG	1 cut(s)	1010
BpuMI	CCSGG	2 cut(s)	1206, 1293
Bsa29I	ATCGAT	1 cut(s)	582
BsaBI	GATNNNNATC	1 cut(s)	588
BsaJI	CCNNGG	4 cut(s)	462, 695, 1112, 1205
BsaWI	WCCGGW	1 cut(s)	325
Bsc4I	CCNNNNNNNGG	5 cut(s)	307, 569, 593, 594, 1168
Bse1I	ACTGG	3 cut(s)	212, 312, 1006
Bse21I	CCTNAGG	1 cut(s)	721
Bse3DI	GCAATG	1 cut(s)	773
Bse8I	GATNNNNATC	1 cut(s)	588
BseCI	ATCGAT	1 cut(s)	582
BseDI	CCNNGG	4 cut(s)	462, 695, 1112, 1205
BseGI	GGATG	1 cut(s)	1215
BseJI	GATNNNNATC	1 cut(s)	588
BseLI	CCNNNNNNNGG	5 cut(s)	307, 569, 593, 594, 1168
BseMI	GCAATG	1 cut(s)	773
BseMII	CTCAG	4 cut(s)	120, 753, 915, 1326
BseNI	ACTGG	3 cut(s)	212, 312, 1006
BseRI	GAGGAG	4 cut(s)	27, 356, 426, 1112
BseXI	GCAGC	4 cut(s)	642, 850, 1153, 1184
Bsh1236I	CGCG	2 cut(s)	360, 379
BshFI	GGCC	3 cut(s)	300, 332, 467
BshVI	ATCGAT	1 cut(s)	582
BsiSI	CCGG	4 cut(s)	326, 1205, 1273, 1292
BslI	CCNNNNNNNGG	5 cut(s)	307, 569, 593, 594, 1168
BsmAI	GTCTC	3 cut(s)	224, 254, 777
BsmBI	CGTCTC	1 cut(s)	224
BsnI	GGCC	3 cut(s)	300, 332, 467
Bsp1407I	TGTACA	1 cut(s)	609
Bsp143I	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
BspACI	CCGC	5 cut(s)	358, 861, 1169, 1239, 1313
BspANI	GGCC	3 cut(s)	300, 332, 467
BspCNI	CTCAG	4 cut(s)	119, 754, 916, 1327
BspDI	ATCGAT	1 cut(s)	582
BspFNI	CGCG	2 cut(s)	360, 379
BspLI	GGNNCC	2 cut(s)	876, 921
BspPI	GGATC	5 cut(s)	232, 337, 578, 1103, 1153
BsrDI	GCAATG	1 cut(s)	773
BsrGI	TGTACA	1 cut(s)	609
BsrI	ACTGG	3 cut(s)	212, 312, 1006
BssECI	CCNNGG	4 cut(s)	462, 695, 1112, 1205
BssMI	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
BssSI	CACGAG	1 cut(s)	51
BssT1I	CCWWGG	2 cut(s)	695, 1112
Bst2BI	CACGAG	1 cut(s)	51
Bst4CI	ACNGT	5 cut(s)	156, 235, 895, 1068, 1307
Bst6I	CTCTTC	4 cut(s)	308, 403, 662, 1337
BstAUI	TGTACA	1 cut(s)	609
BstC8I	GCNNGC	2 cut(s)	535, 1359
BstDEI	CTNAG	6 cut(s)	106, 721, 762, 924, 1256, 1335
BstDSI	CCRYGG	1 cut(s)	462
BstENI	CCTNNNNNAGG	1 cut(s)	567
BstF5I	GGATG	1 cut(s)	1215
BstFNI	CGCG	2 cut(s)	360, 379
BstHHI	GCGC	2 cut(s)	381, 868
BstKTI	GATC	8 cut(s)	227, 345, 586, 592, 1111, 1161, 1185, 1396
BstMAI	GTCTC	3 cut(s)	224, 254, 777
BstMBI	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
BstMWI	GCNNNNNNNGC	3 cut(s)	639, 1172, 1363
BstSCI	CCNGG	2 cut(s)	1204, 1291
BstSFI	CTRYAG	1 cut(s)	1092
BstUI	CGCG	2 cut(s)	360, 379
BstV1I	GCAGC	4 cut(s)	642, 850, 1153, 1184
BstV2I	GAAGAC	4 cut(s)	120, 683, 1033, 1207
BstX2I	RGATCY	1 cut(s)	1108
BstYI	RGATCY	1 cut(s)	1108
Bsu15I	ATCGAT	1 cut(s)	582
Bsu36I	CCTNAGG	1 cut(s)	721
BsuRI	GGCC	3 cut(s)	300, 332, 467
BsuTUI	ATCGAT	1 cut(s)	582
BtgI	CCRYGG	1 cut(s)	462
BtgZI	GCGATG	1 cut(s)	1376
BtsCI	GGATG	1 cut(s)	1215
BtsI	GCAGTG	1 cut(s)	369
BtsIMutI	CAGTG	4 cut(s)	219, 305, 369, 759
Cac8I	GCNNGC	2 cut(s)	535, 1359
CaiI	CAGNNNCTG	1 cut(s)	218
CfoI	GCGC	2 cut(s)	381, 868
Cfr13I	GGNCC	1 cut(s)	858
ClaI	ATCGAT	1 cut(s)	582
Csp6I	GTAC	3 cut(s)	610, 709, 1125
CviAII	CATG	1 cut(s)	650
CviQI	GTAC	3 cut(s)	610, 709, 1125
DdeI	CTNAG	6 cut(s)	106, 721, 762, 924, 1256, 1335
DpnI	GATC	8 cut(s)	226, 344, 585, 591, 1110, 1160, 1184, 1395
DpnII	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
EaeI	YGGCCR	2 cut(s)	330, 465
Eam1104I	CTCTTC	4 cut(s)	308, 403, 662, 1337
EarI	CTCTTC	4 cut(s)	308, 403, 662, 1337
Eco130I	CCWWGG	2 cut(s)	695, 1112
Eco147I	AGGCCT	1 cut(s)	300
Eco32I	GATATC	1 cut(s)	948
Eco47I	GGWCC	1 cut(s)	858
Eco57I	CTGAAG	1 cut(s)	240
Eco81I	CCTNAGG	1 cut(s)	721
EcoNI	CCTNNNNNAGG	1 cut(s)	567
EcoRV	GATATC	1 cut(s)	948
EcoT14I	CCWWGG	2 cut(s)	695, 1112
ErhI	CCWWGG	2 cut(s)	695, 1112
Esp3I	CGTCTC	1 cut(s)	224
FaeI	CATG	1 cut(s)	653
FaiI	YATR	9 cut(s)	426, 618, 651, 740, 753, 780, 1077, 1157, 1298
FalI	AAGNNNNNCTT	8 cut(s)	57, 89, 400, 432, 668, 700, 704, 736
FatI	CATG	1 cut(s)	649
FbaI	TGATCA	1 cut(s)	1182
FblI	GTMKAC	1 cut(s)	701
Fnu4HI	GCNGC	5 cut(s)	631, 864, 1167, 1170, 1173
FokI	GGATG	1 cut(s)	1222
Fsp4HI	GCNGC	5 cut(s)	631, 864, 1167, 1170, 1173
FspBI	CTAG	4 cut(s)	242, 569, 1106, 1128
GlaI	GCGC	2 cut(s)	380, 867
GluI	GCNGC	5 cut(s)	631, 864, 1167, 1170, 1173
GsuI	CTGGAG	3 cut(s)	185, 272, 329
HaeIII	GGCC	3 cut(s)	300, 332, 467
HapII	CCGG	4 cut(s)	326, 1205, 1273, 1292
HhaI	GCGC	2 cut(s)	381, 868
Hin1II	CATG	1 cut(s)	653
Hin6I	GCGC	2 cut(s)	379, 866
HinP1I	GCGC	2 cut(s)	379, 866
HindIII	AAGCTT	2 cut(s)	66, 95
HpaII	CCGG	4 cut(s)	326, 1205, 1273, 1292
HphI	GGTGA	3 cut(s)	290, 1049, 1253
Hpy166II	GTNNAC	3 cut(s)	610, 702, 967
Hpy188III	TCNNGA	8 cut(s)	8, 53, 251, 294, 445, 587, 923, 1197
Hpy8I	GTNNAC	3 cut(s)	610, 702, 967
HpyAV	CCTTC	5 cut(s)	198, 215, 879, 1207, 1238
HpyCH4III	ACNGT	5 cut(s)	156, 235, 895, 1068, 1307
HpyCH4IV	ACGT	2 cut(s)	555, 1262
HpyCH4V	TGCA	7 cut(s)	124, 516, 533, 731, 778, 1075, 1321
HpyF10VI	GCNNNNNNNGC	3 cut(s)	639, 1172, 1363
HpyF3I	CTNAG	6 cut(s)	106, 721, 762, 924, 1256, 1335
HpySE526I	ACGT	2 cut(s)	555, 1262
Hsp92II	CATG	1 cut(s)	653
HspAI	GCGC	2 cut(s)	379, 866
Ksp22I	TGATCA	1 cut(s)	1182
Kzo9I	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
LmnI	GCTCC	4 cut(s)	160, 413, 925, 1150
Lsp1109I	GCAGC	4 cut(s)	642, 850, 1153, 1184
LweI	GCATC	2 cut(s)	724, 1353
MaeI	CTAG	4 cut(s)	242, 569, 1106, 1128
MaeII	ACGT	2 cut(s)	555, 1262
MaeIII	GTNAC	2 cut(s)	353, 1372
MalI	GATC	8 cut(s)	226, 344, 585, 591, 1110, 1160, 1184, 1395
MboI	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
MfeI	CAATTG	1 cut(s)	1014
MflI	RGATCY	1 cut(s)	1108
MlsI	TGGCCA	1 cut(s)	467
MluCI	AATT	8 cut(s)	205, 269, 286, 382, 822, 1014, 1045, 1379
MluNI	TGGCCA	1 cut(s)	467
MlyI	GAGTC	3 cut(s)	98, 987, 1354
MmeI	TCCRAC	3 cut(s)	138, 958, 986
Mox20I	TGGCCA	1 cut(s)	467
MscI	TGGCCA	1 cut(s)	467
MseI	TTAA	4 cut(s)	174, 432, 543, 789
Msp20I	TGGCCA	1 cut(s)	467
MspA1I	CMGCKG	1 cut(s)	863
MspI	CCGG	4 cut(s)	326, 1205, 1273, 1292
MspR9I	CCNGG	2 cut(s)	1206, 1293
MunI	CAATTG	1 cut(s)	1014
MvnI	CGCG	2 cut(s)	360, 379
MwoI	GCNNNNNNNGC	3 cut(s)	639, 1172, 1363
NciI	CCSGG	2 cut(s)	1206, 1293
NdeII	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
NlaIII	CATG	1 cut(s)	653
NlaIV	GGNNCC	2 cut(s)	876, 921
NmeAIII	GCCGAG	1 cut(s)	1118
PceI	AGGCCT	1 cut(s)	300
PfeI	GAWTC	8 cut(s)	4, 247, 335, 348, 441, 497, 688, 1193
PkrI	GCNGC	5 cut(s)	632, 865, 1168, 1171, 1174
PleI	GAGTC	3 cut(s)	98, 986, 1353
PpsI	GAGTC	3 cut(s)	98, 986, 1353
Psp1406I	AACGTT	1 cut(s)	555
PspN4I	GGNNCC	2 cut(s)	876, 921
PspPI	GGNCC	1 cut(s)	858
PsrI	GAACNNNNNNTAC	2 cut(s)	630, 662
PstNI	CAGNNNCTG	1 cut(s)	218
PsuI	RGATCY	1 cut(s)	1108
RsaI	GTAC	3 cut(s)	611, 710, 1126
RsaNI	GTAC	3 cut(s)	610, 709, 1125
SaqAI	TTAA	4 cut(s)	174, 432, 543, 789
SatI	GCNGC	5 cut(s)	631, 864, 1167, 1170, 1173
Sau3AI	GATC	8 cut(s)	224, 342, 583, 589, 1108, 1158, 1182, 1393
Sau96I	GGNCC	1 cut(s)	858
SchI	GAGTC	3 cut(s)	98, 987, 1354
ScrFI	CCNGG	2 cut(s)	1206, 1293
SfaNI	GCATC	2 cut(s)	724, 1353
SfcI	CTRYAG	1 cut(s)	1092
SinI	GGWCC	1 cut(s)	858
SmlI	CTYRAG	1 cut(s)	989
SmoI	CTYRAG	1 cut(s)	989
Sse9I	AATT	8 cut(s)	205, 269, 286, 382, 822, 1014, 1045, 1379
SseBI	AGGCCT	1 cut(s)	300
SsiI	CCGC	5 cut(s)	358, 861, 1169, 1239, 1313
SspI	AATATT	1 cut(s)	802
SspMI	CTAG	4 cut(s)	242, 569, 1106, 1128
StuI	AGGCCT	1 cut(s)	300
StyD4I	CCNGG	2 cut(s)	1204, 1291
StyI	CCWWGG	2 cut(s)	695, 1112
TaaI	ACNGT	5 cut(s)	156, 235, 895, 1068, 1307
TaiI	ACGT	2 cut(s)	558, 1265
TaqI	TCGA	5 cut(s)	40, 293, 582, 636, 1145
TaqII	GACCGA	1 cut(s)	974
TasI	AATT	8 cut(s)	205, 269, 286, 382, 822, 1014, 1045, 1379
TatI	WGTACW	2 cut(s)	609, 1124
TauI	GCSGC	1 cut(s)	1172
TfiI	GAWTC	8 cut(s)	4, 247, 335, 348, 441, 497, 688, 1193
Tru1I	TTAA	4 cut(s)	174, 432, 543, 789
Tru9I	TTAA	4 cut(s)	174, 432, 543, 789
TscAI	CASTG	4 cut(s)	219, 312, 376, 766
TseI	GCWGC	4 cut(s)	630, 863, 1166, 1172
TspDTI	ATGAA	3 cut(s)	299, 1226, 1355
TspGWI	ACGGA	3 cut(s)	409, 1339, 1391
TspRI	CASTG	4 cut(s)	219, 312, 376, 766
VpaK11BI	GGWCC	1 cut(s)	858
XagI	CCTNNNNNAGG	1 cut(s)	567
XapI	RAATTY	4 cut(s)	205, 822, 1045, 1379
XmiI	GTMKAC	1 cut(s)	701
XspI	CTAG	4 cut(s)	242, 569, 1106, 1128

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.