Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0008391

Physical Location & Seq

Genomic Coordinates Forward (+)

33584528 .. 33587518

Gene Structure 2991 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1262600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1590 bp

ATGGGTAACTCCCAAGGCACCTTCACCAACTGCAGAGAGCCACCCAATGCCCTTCCTCCTCCTCTTCCTCTCTACACTTCCTCCTCTGTAGCCACAGATCACAACTACTGTGACTACAATGACCACAGAATCAAAACCCTTGTGAAGAAAATGTTGAGGGAGTTTGGCTTGTCCTGTTTTCTCCCACCTCGCCAGAAAAAGAGCAACCAGAAGACCCCAGTTGATGACGACAGCAACAACAACAAGAAGATAAATTTAGAGCACAACAAAGCGTGGCTGCTGGCGGAGTCCGGCGGGTGTGGGGCGGAGTTGGAAAATGCCGATCCCCATTCGGTTAACTCCTCCTTCAGATTCACCTTCTGCTCTCAGGTGGAGCTGGATTCCATGAACATGAGCTCCTCCGCCGCTGCAACGGTTCTAATGGTGAATTTGGGAAATGGGATGTCTGAATCCAAAGCTAAAGAGGTCAAGTGGAGGCGATTTGAGTCGTTGGAGAGGAGCATTTCCCCAATGGCTCACACTTTGATCAGATTTAGCTACGGTGAAATTCTTGCTGCCACTCGCAATTTTTCGAAAGGTAGAGTTTTGGGGAGGGGAGCTTTGAGCTGTGTATTTAGGGGCAGAGTTGGGATTTTCAGAACTGCAGTGGCGATCAAGCGACTGGACAAGGAAGATAAAGAGTCTGCTAAGGCATTTTGTAGAGAGTTGATGATTTCTAGCTCTCTTCACAACCCAAATGTGACACCTCTTGTGGGGTTTTGCATTGATCCGGAGGAGGGCTTGTTTTTGGTTTACAAGTATGTCTCCGGGGGAAGCTTGGAGCGTCATTTGCACGAGAAAACGAGGGGAGGTAAGGGCTCTGCAACACTTCCTTGGTTTGTAAGGCACAAAGTTGCCATTGGAATTGCTGAGGCAATTGCGTATCTGCATAATGGAACCGAACGATGTATTGTTCACAGAGATATCAAACCCTCAAACATTCTCCTTTCGTCCAAGAAAACCGCCAAGTTATGTGATTTTGGATTAGCCACTTGGACTTCCGCACCTTCCGTTCCCTTCCTTTGCAAAACTGTCAAAGGAACATTTGGTTACTTGGCTCCTGAGTACTTCCAGCATGGGAAAATATCGGATAAAACCGATGTGTATGCTTTTGGAGTGGTCTTGCTGGAATTGATAACCGGAAGGAAGCCAATTGAAGCAAGAAGGCCACAAGGAGAAGAAAACTTGGTTCTCTGGGCAAAGCCCCTCTTGTGTAAAGGGAGAGGAGCTATCGAGGAGCTGCTTGATGCACGAATAAAATGCACTTCGAGAAATTCAAATCAAATTGCACGGATGATTGAAGCTGCAGCTGCCTGCGTAACGAGTGAAGAATCCCGGAGGCCAAGCATCTGTGAGATAATGGCAATACTGAAAGGCGAAGAAGAACCTAGCGTCTCCAAGAGGAAGAGGCCTAGTTTTCTGGGGAATGGATTGTCGATTGATTGTTATTCTCAATTACAACAAACGAATAGCGAGATGAAGAGTCACCTGGCTTTGGCTATGTTAGGAGTTTCCGAGTTTGAAGATGATGATCATCTTTACGGGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0036211 GO:0038023 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

530

Amino Acids

58.66

Weight (kDa)

8.97

Isoelectric Point (pI)

46.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	191 - 467	8.5e-44	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	193 - 468	2.6e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008391)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G66460 AT1G66460 AT1G66460 AT5G37790
fragaria_vesca	FvH4_1g28910 FvH4_1g28910
malus_domestica	MD09G1262600.v1.1 MD17G1257500.v1.1
prunus_persica	Prupe.3G160300_v2.0.a1 Prupe.3G160300_v2.0.a1 Prupe.3G160300_v2.0.a1
pyrus_communis	pycom09g17690 pycom17g25960
rosa_chinensis	RchiOBHm_Chr3g0493411
rosa_laevigata	RLG00000022715
rosa_multiflora	Rmu_sc0000828.1_g000022
rosa_roxburghii	Rroxscaffold_6G00391600
rosa_rugosa	Rorug03G0260900
rosa_samantha	Rh3AG310000 Rh3BG346100 Rh3CG343500 Rh3DG345100
rosa_wichuraiana	Rw3G027230

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	17
AccIII	TCCGGA	1 cut(s)	769
AciI	CCGC	7 cut(s)	284, 294, 305, 402, 405, 1002, 1041
AclWI	GGATC	2 cut(s)	317, 761
AcsI	RAATTY	4 cut(s)	253, 427, 546, 1312
AcuI	CTGAAG	1 cut(s)	331
AfaI	GTAC	1 cut(s)	1106
AfiI	CCNNNNNNNGG	3 cut(s)	752, 776, 1534
AgsI	TTSAA	4 cut(s)	1196, 1317, 1340, 1562
AjnI	CCWGG	1 cut(s)	1527
AloI	GAACNNNNNNTCC	2 cut(s)	380, 412
Alw21I	GWGCWC	2 cut(s)	264, 398
Alw26I	GTCTC	2 cut(s)	808, 1438
AlwI	GGATC	2 cut(s)	317, 761
Aor13HI	TCCGGA	1 cut(s)	769
AoxI	GGCC	3 cut(s)	1205, 1379, 1448
ApeKI	GCWGC	7 cut(s)	277, 407, 554, 1279, 1343, 1346, 1349
ApoI	RAATTY	4 cut(s)	253, 427, 546, 1312
AsuC2I	CCSGG	2 cut(s)	808, 1375
AsuHPI	GGTGA	5 cut(s)	16, 346, 436, 554, 1517
AsuII	TTCGAA	1 cut(s)	572
BanI	GGYRCC	1 cut(s)	17
BanII	GRGCYC	2 cut(s)	398, 860
BauI	CACGAG	1 cut(s)	833
BbsI	GAAGAC	1 cut(s)	218
Bbv12I	GWGCWC	2 cut(s)	264, 398
BbvCI	CCTCAGC	1 cut(s)	909
BbvI	GCAGC	7 cut(s)	264, 394, 541, 1266, 1330, 1336, 1358
BcgI	CGANNNNNNTGC	4 cut(s)	1127, 1161, 1281, 1315
BciT130I	CCWGG	1 cut(s)	1529
BclI	TGATCA	2 cut(s)	525, 1570
BcnI	CCSGG	2 cut(s)	808, 1375
BcoDI	GTCTC	2 cut(s)	808, 1438
BfaI	CTAG	3 cut(s)	717, 1428, 1452
BfmI	CTRYAG	4 cut(s)	31, 87, 642, 1344
BisI	GCNGC	8 cut(s)	278, 405, 408, 555, 1280, 1344, 1347, 1350
BlsI	GCNGC	8 cut(s)	279, 406, 409, 556, 1281, 1345, 1348, 1351
BmcAI	AGTACT	1 cut(s)	1106
Bme1390I	CCNGG	3 cut(s)	808, 1375, 1529
BmiI	GGNNCC	3 cut(s)	19, 937, 1098
BmrFI	CCNGG	3 cut(s)	808, 1375, 1529
BmrI	ACTGGG	1 cut(s)	212
BmsI	GCATC	2 cut(s)	1276, 1395
BmuI	ACTGGG	1 cut(s)	212
BpiI	GAAGAC	1 cut(s)	218
Bpu10I	CCTNAGC	2 cut(s)	687, 909
Bpu14I	TTCGAA	1 cut(s)	572
BpuMI	CCSGG	2 cut(s)	808, 1375
BsaBI	GATNNNNATC	2 cut(s)	1569, 1572
BsaJI	CCNNGG	3 cut(s)	13, 807, 872
BsaWI	WCCGGW	2 cut(s)	769, 1178
BsaXI	ACNNNNNCTCC	6 cut(s)	65, 95, 380, 410, 767, 797
Bsc4I	CCNNNNNNNGG	3 cut(s)	752, 776, 1534
Bse1I	ACTGG	2 cut(s)	218, 666
Bse8I	GATNNNNATC	2 cut(s)	1569, 1572
BseAI	TCCGGA	1 cut(s)	769
BseBI	CCWGG	1 cut(s)	1529
BseDI	CCNNGG	3 cut(s)	13, 807, 872
BseGI	GGATG	2 cut(s)	447, 1338
BseJI	GATNNNNATC	2 cut(s)	1569, 1572
BseLI	CCNNNNNNNGG	3 cut(s)	752, 776, 1534
BseMII	CTCAG	3 cut(s)	380, 900, 1092
BseNI	ACTGG	2 cut(s)	218, 666
BseRI	GAGGAG	9 cut(s)	48, 51, 73, 331, 388, 511, 788, 1278, 1289
BseXI	GCAGC	7 cut(s)	264, 394, 541, 1266, 1330, 1336, 1358
BshFI	GGCC	3 cut(s)	1207, 1381, 1450
BshNI	GGYRCC	1 cut(s)	17
BsiHKAI	GWGCWC	2 cut(s)	264, 398
BsiSI	CCGG	5 cut(s)	291, 770, 807, 1179, 1375
BslI	CCNNNNNNNGG	3 cut(s)	752, 776, 1534
BsmAI	GTCTC	2 cut(s)	808, 1438
BsmBI	CGTCTC	1 cut(s)	1438
BsnI	GGCC	3 cut(s)	1207, 1381, 1450
Bsp119I	TTCGAA	1 cut(s)	572
Bsp1286I	GDGCHC	3 cut(s)	264, 398, 860
Bsp13I	TCCGGA	1 cut(s)	769
Bsp143I	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
BspACI	CCGC	7 cut(s)	284, 294, 305, 402, 405, 1002, 1041
BspANI	GGCC	3 cut(s)	1207, 1381, 1450
BspCNI	CTCAG	3 cut(s)	379, 901, 1093
BspEI	TCCGGA	1 cut(s)	769
BspLI	GGNNCC	3 cut(s)	19, 937, 1098
BspMAI	CTGCAG	3 cut(s)	35, 646, 1348
BspPI	GGATC	2 cut(s)	317, 761
BspT104I	TTCGAA	1 cut(s)	572
BspT107I	GGYRCC	1 cut(s)	17
BsrI	ACTGG	2 cut(s)	218, 666
BssECI	CCNNGG	3 cut(s)	13, 807, 872
BssMI	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
BssSI	CACGAG	1 cut(s)	833
BssT1I	CCWWGG	2 cut(s)	13, 872
Bst2BI	CACGAG	1 cut(s)	833
Bst2UI	CCWGG	1 cut(s)	1529
Bst4CI	ACNGT	4 cut(s)	110, 415, 542, 1072
Bst6I	CTCTTC	4 cut(s)	69, 729, 1439, 1514
BstBI	TTCGAA	1 cut(s)	572
BstC8I	GCNNGC	2 cut(s)	282, 1354
BstDEI	CTNAG	4 cut(s)	366, 687, 909, 1101
BstF5I	GGATG	2 cut(s)	447, 1338
BstKTI	GATC	6 cut(s)	100, 325, 528, 654, 769, 1573
BstMAI	GTCTC	2 cut(s)	808, 1438
BstMBI	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
BstMWI	GCNNNNNNNGC	2 cut(s)	829, 1349
BstNI	CCWGG	1 cut(s)	1529
BstSCI	CCNGG	3 cut(s)	806, 1373, 1527
BstSFI	CTRYAG	4 cut(s)	31, 87, 642, 1344
BstV1I	GCAGC	7 cut(s)	264, 394, 541, 1266, 1330, 1336, 1358
BstV2I	GAAGAC	1 cut(s)	218
BsuRI	GGCC	3 cut(s)	1207, 1381, 1450
BtsCI	GGATG	2 cut(s)	447, 1338
BtsI	GCAGTG	1 cut(s)	651
BtsIMutI	CAGTG	1 cut(s)	651
Cac8I	GCNNGC	2 cut(s)	282, 1354
CseI	GACGC	2 cut(s)	812, 1420
Csp6I	GTAC	1 cut(s)	1105
CviAII	CATG	3 cut(s)	385, 391, 1115
CviQI	GTAC	1 cut(s)	1105
DdeI	CTNAG	4 cut(s)	366, 687, 909, 1101
DpnI	GATC	6 cut(s)	99, 324, 527, 653, 768, 1572
DpnII	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
Eam1104I	CTCTTC	4 cut(s)	69, 729, 1439, 1514
EarI	CTCTTC	4 cut(s)	69, 729, 1439, 1514
EciI	GGCGGA	3 cut(s)	299, 320, 391
Ecl136II	GAGCTC	1 cut(s)	396
Eco130I	CCWWGG	2 cut(s)	13, 872
Eco147I	AGGCCT	1 cut(s)	1450
Eco24I	GRGCYC	2 cut(s)	398, 860
Eco32I	GATATC	1 cut(s)	964
Eco53kI	GAGCTC	1 cut(s)	396
Eco57I	CTGAAG	1 cut(s)	331
EcoICRI	GAGCTC	1 cut(s)	396
EcoRII	CCWGG	1 cut(s)	1527
EcoRV	GATATC	1 cut(s)	964
EcoT14I	CCWWGG	2 cut(s)	13, 872
EcoT38I	GRGCYC	2 cut(s)	398, 860
ErhI	CCWWGG	2 cut(s)	13, 872
Esp3I	CGTCTC	1 cut(s)	1438
FaeI	CATG	3 cut(s)	388, 394, 1118
FaiI	YATR	8 cut(s)	386, 392, 801, 930, 1012, 1116, 1146, 1541
FalI	AAGNNNNNCTT	2 cut(s)	1232, 1264
FatI	CATG	3 cut(s)	384, 390, 1114
FauI	CCCGC	1 cut(s)	287
FbaI	TGATCA	2 cut(s)	525, 1570
Fnu4HI	GCNGC	8 cut(s)	278, 405, 408, 555, 1280, 1344, 1347, 1350
FokI	GGATG	2 cut(s)	454, 1345
FriOI	GRGCYC	2 cut(s)	398, 860
Fsp4HI	GCNGC	8 cut(s)	278, 405, 408, 555, 1280, 1344, 1347, 1350
FspBI	CTAG	3 cut(s)	717, 1428, 1452
GluI	GCNGC	8 cut(s)	278, 405, 408, 555, 1280, 1344, 1347, 1350
HaeIII	GGCC	3 cut(s)	1207, 1381, 1450
HapII	CCGG	5 cut(s)	291, 770, 807, 1179, 1375
HgaI	GACGC	2 cut(s)	812, 1420
Hin1II	CATG	3 cut(s)	388, 394, 1118
HincII	GTYRAC	1 cut(s)	337
HindII	GTYRAC	1 cut(s)	337
HindIII	AAGCTT	1 cut(s)	814
HinfI	GANTC	9 cut(s)	129, 287, 351, 380, 449, 485, 680, 1370, 1522
HpaI	GTTAAC	1 cut(s)	337
HpaII	CCGG	5 cut(s)	291, 770, 807, 1179, 1375
HphI	GGTGA	5 cut(s)	16, 346, 436, 554, 1517
Hpy166II	GTNNAC	3 cut(s)	337, 793, 955
Hpy188I	TCNGA	6 cut(s)	350, 448, 530, 638, 1129, 1555
Hpy188III	TCNNGA	3 cut(s)	770, 1100, 1308
Hpy8I	GTNNAC	3 cut(s)	337, 793, 955
HpyAV	CCTTC	8 cut(s)	31, 62, 355, 367, 1056, 1066, 1176, 1197
HpyCH4III	ACNGT	4 cut(s)	110, 415, 542, 1072
HpyF10VI	GCNNNNNNNGC	2 cut(s)	829, 1349
HpyF3I	CTNAG	4 cut(s)	366, 687, 909, 1101
Hsp92II	CATG	3 cut(s)	388, 394, 1118
Kpn2I	TCCGGA	1 cut(s)	769
Ksp22I	TGATCA	2 cut(s)	525, 1570
KspAI	GTTAAC	1 cut(s)	337
Kzo9I	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
LmnI	GCTCC	8 cut(s)	373, 401, 498, 596, 820, 1102, 1265, 1276
Lsp1109I	GCAGC	7 cut(s)	264, 394, 541, 1266, 1330, 1336, 1358
LweI	GCATC	2 cut(s)	1276, 1395
MaeI	CTAG	3 cut(s)	717, 1428, 1452
MaeIII	GTNAC	6 cut(s)	5, 110, 739, 1088, 1357, 1523
MalI	GATC	6 cut(s)	99, 324, 527, 653, 768, 1572
MboI	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
MfeI	CAATTG	2 cut(s)	915, 1191
MhlI	GDGCHC	3 cut(s)	264, 398, 860
MlyI	GAGTC	4 cut(s)	296, 494, 689, 1531
MmeI	TCCRAC	2 cut(s)	291, 471
MroI	TCCGGA	1 cut(s)	769
MseI	TTAA	1 cut(s)	336
MslI	CAYNNNNRTG	1 cut(s)	389
MspA1I	CMGCKG	2 cut(s)	407, 1349
MspI	CCGG	5 cut(s)	291, 770, 807, 1179, 1375
MspR9I	CCNGG	3 cut(s)	808, 1375, 1529
MunI	CAATTG	2 cut(s)	915, 1191
MvaI	CCWGG	1 cut(s)	1529
MwoI	GCNNNNNNNGC	2 cut(s)	829, 1349
NciI	CCSGG	2 cut(s)	808, 1375
NdeII	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
NlaIII	CATG	3 cut(s)	388, 394, 1118
NlaIV	GGNNCC	3 cut(s)	19, 937, 1098
NmuCI	GTSAC	3 cut(s)	110, 739, 1523
NspV	TTCGAA	1 cut(s)	572
PceI	AGGCCT	1 cut(s)	1450
PfeI	GAWTC	5 cut(s)	129, 351, 380, 449, 1370
PfoI	TCCNGGA	1 cut(s)	1373
PkrI	GCNGC	8 cut(s)	279, 406, 409, 556, 1281, 1345, 1348, 1351
PleI	GAGTC	4 cut(s)	295, 493, 688, 1530
PpsI	GAGTC	4 cut(s)	295, 493, 688, 1530
Psp124BI	GAGCTC	1 cut(s)	398
Psp6I	CCWGG	1 cut(s)	1527
PspGI	CCWGG	1 cut(s)	1527
PspN4I	GGNNCC	3 cut(s)	19, 937, 1098
PstI	CTGCAG	3 cut(s)	35, 646, 1348
PvuII	CAGCTG	1 cut(s)	1349
RsaI	GTAC	1 cut(s)	1106
RsaNI	GTAC	1 cut(s)	1105
RseI	CAYNNNNRTG	1 cut(s)	389
SacI	GAGCTC	1 cut(s)	398
SaqAI	TTAA	1 cut(s)	336
SatI	GCNGC	8 cut(s)	278, 405, 408, 555, 1280, 1344, 1347, 1350
Sau3AI	GATC	6 cut(s)	97, 322, 525, 651, 766, 1570
ScaI	AGTACT	1 cut(s)	1106
SchI	GAGTC	4 cut(s)	296, 494, 689, 1531
ScrFI	CCNGG	3 cut(s)	808, 1375, 1529
SduI	GDGCHC	3 cut(s)	264, 398, 860
SfaNI	GCATC	2 cut(s)	1276, 1395
SfcI	CTRYAG	4 cut(s)	31, 87, 642, 1344
SfuI	TTCGAA	1 cut(s)	572
SmiMI	CAYNNNNRTG	1 cut(s)	389
SseBI	AGGCCT	1 cut(s)	1450
SsiI	CCGC	7 cut(s)	284, 294, 305, 402, 405, 1002, 1041
SspMI	CTAG	3 cut(s)	717, 1428, 1452
SstI	GAGCTC	1 cut(s)	398
StuI	AGGCCT	1 cut(s)	1450
StyD4I	CCNGG	3 cut(s)	806, 1373, 1527
StyI	CCWWGG	2 cut(s)	13, 872
TaaI	ACNGT	4 cut(s)	110, 415, 542, 1072
TaqI	TCGA	4 cut(s)	572, 1272, 1307, 1475
TatI	WGTACW	1 cut(s)	1104
TauI	GCSGC	1 cut(s)	407
TfiI	GAWTC	5 cut(s)	129, 351, 380, 449, 1370
Tru1I	TTAA	1 cut(s)	336
Tru9I	TTAA	1 cut(s)	336
TscAI	CASTG	1 cut(s)	651
TseFI	GTSAC	3 cut(s)	110, 739, 1523
TseI	GCWGC	7 cut(s)	277, 407, 554, 1279, 1343, 1346, 1349
Tsp45I	GTSAC	3 cut(s)	110, 739, 1523
TspDTI	ATGAA	2 cut(s)	401, 1532
TspGWI	ACGGA	2 cut(s)	1039, 1345
TspRI	CASTG	1 cut(s)	651
XapI	RAATTY	4 cut(s)	253, 427, 546, 1312
XspI	CTAG	3 cut(s)	717, 1428, 1452
ZrmI	AGTACT	1 cut(s)	1106

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.