AT1G66470

core promoter sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0012195
Physical Location & Seq
Forward (+)
24795214 .. 24797015
1802 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G66470.1

Sequence Viewer

Length: 897 bp
ATGGCACTCGTTAATGACCATCCCAACGAGACCAATTACTTGTCAAAACAAAATTCCTCCTCTTCCGAAGATCTCTCCTCGCCGGGACTGGATCAGCCAGATGCAGCTTATGCCGGTGGAGGAGGAGGAGGAGGCTCGGCTTCGAGCAGTAGCACGATGAATTCAGATCATCAACAACATCAGGGGTTTGTATTTTACCCATCCGGTGAAGATCATCACAACTCTTTGATGGATTTCAACGGATCATCATTTCTTAACTTTGATCATCACGAGAGCTTTCCTCCTCCAGCCATAAGCTGTGGTGGTAGTAGCGGTGGGGGCGGCTTCTCCTTCTTGGAGGGCAACAACATGAGCTACGGCTTCACAAACTGGAATCATCAACATCATATGGATATTATTAGCCCTAGATCCACCGAAACTCCCCAAGGCCAGAAAGACTGGTTATATTCTGATTCAACTGTTGTAACCACTGGTTCTAGAAACGAGTCTCTTTCGCCTAAATCCGCTGGAAACAAACGTTCTCACACGGGAGAGAGCACTCAACCGTCGAAGAAACTGAGTAGCGGTGTGACCGGAAAGACCAAGCCTAAGCCAACAACTTCACCTAAAGATCCACAAAGCCTAGCAGCCAAGAATCGAAGAGAAAGGATAAGTGAACGTCTCAAGATATTGCAAGAACTTGTTCCCAATGGCACCAAGGTTGATTTGGTGACAATGCTTGAAAAGGCTATTAGTTATGTCAAGTTCCTTCAAGTACAAGTTAAGGTATTAGCGACCGATGAGTTTTGGCCGGCTCAAGGAGGAAAAGCTCCTGACATTTCTCAAGTTAAAGACGCCATTGATGCCATTCTCTCCTCATCACAACGAGACAGGAATTCGAATCTGATCACCAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000902 GO:0000904 GO:0000976 GO:0000981 GO:0001046 GO:0001047 GO:0001067 GO:0001228 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0006139 GO:0006351 GO:0006355 GO:0006357 GO:0006366 GO:0006725 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009653 GO:0009888 GO:0009889 GO:0009891 GO:0009893 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0010467 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0016043 GO:0016070 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0021700 GO:0022622 GO:0030154 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0032501 GO:0032502 GO:0032774 GO:0032989 GO:0034641 GO:0034645 GO:0034654 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043565 GO:0044212 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044424 GO:0044444 GO:0044464 GO:0045893 GO:0045935 GO:0045944 GO:0046483 GO:0048364 GO:0048468 GO:0048469 GO:0048518 GO:0048522 GO:0048731 GO:0048764 GO:0048765 GO:0048766 GO:0048856 GO:0048869 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0071695 GO:0071704 GO:0071840 GO:0080090 GO:0090304 GO:0090558 GO:0090627 GO:0097159 GO:0097659 GO:0099402 GO:0140110 GO:1901360 GO:1901362 GO:1901363 GO:1901576 GO:1902680 GO:1903506 GO:1903508 GO:1905392 GO:1990837 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HLH

Protein Analysis

298

Amino Acids

32.09

Weight (kDa)

6.25

Isoelectric Point (pI)

53.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HLH PF00010 210 - 251 1.6e-08 Helix-loop-helix DNA-binding domain
bHLH_LHW PF23176 214 - 252 1.8e-06 Transcription factor LHW bHLH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012195)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G66470 AT5G37800
fragaria_vesca FvH4_1g28930 FvH4_1g28930
malus_domestica MD09G1262000.v1.1 MD17G1257000.v1.1
prunus_persica Prupe.3G159800_v2.0.a1
pyrus_communis pycom09g17630 pycom17g25930
rosa_chinensis RchiOBHm_Chr3g0493491
rosa_laevigata RLG00000022711
rosa_multiflora Rmu_sc0002343.1_g000071
rosa_roxburghii Rroxscaffold_6G00391560
rosa_rugosa Rorug03G0261100.1
rosa_samantha Rh3AG310400 Rh3BG346600 Rh3CG344400 Rh3DG345700
rosa_wichuraiana Rw3G027280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 692
AciI CCGC 4 cut(s) 312, 321, 504, 564
AclI AACGTT 1 cut(s) 517
AclWI GGATC 4 cut(s) 99, 250, 402, 605
AcoI YGGCCR 1 cut(s) 788
AcsI RAATTY 3 cut(s) 52, 160, 874
AcyI GRCGYC 1 cut(s) 834
AfaI GTAC 1 cut(s) 756
AfiI CCNNNNNNNGG 1 cut(s) 797
AgsI TTSAA 4 cut(s) 238, 456, 722, 752
AluBI AGCT 5 cut(s) 107, 276, 297, 354, 809
AluI AGCT 5 cut(s) 107, 276, 297, 354, 809
Alw21I GWGCWC 1 cut(s) 539
Alw26I GTCTC 4 cut(s) 23, 492, 665, 861
AlwI GGATC 4 cut(s) 99, 250, 402, 605
AoxI GGCC 2 cut(s) 427, 788
ApeKI GCWGC 2 cut(s) 104, 626
ApoI RAATTY 3 cut(s) 52, 160, 874
Asp700I GAANNNNTTC 1 cut(s) 681
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 4 cut(s) 218, 594, 721, 880
AsuII TTCGAA 1 cut(s) 878
BanI GGYRCC 1 cut(s) 692
BauI CACGAG 1 cut(s) 269
Bbv12I GWGCWC 1 cut(s) 539
BbvI GCAGC 2 cut(s) 116, 638
BccI CCATC 3 cut(s) 27, 208, 223
BceAI ACGGC 1 cut(s) 373
BclI TGATCA 2 cut(s) 262, 885
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 4 cut(s) 23, 492, 665, 861
BfaI CTAG 3 cut(s) 405, 477, 623
BglII AGATCT 1 cut(s) 70
BisI GCNGC 3 cut(s) 105, 322, 627
BlsI GCNGC 3 cut(s) 106, 323, 628
Bme1390I CCNGG 1 cut(s) 84
BmiI GGNNCC 1 cut(s) 694
BmrFI CCNGG 1 cut(s) 84
BmsI GCATC 2 cut(s) 91, 832
BplI GAGNNNNNCTC 2 cut(s) 265, 297
BpmI CTGGAG 1 cut(s) 270
Bpu10I CCTNAGC 1 cut(s) 588
Bpu14I TTCGAA 1 cut(s) 878
BpuEI CTTGAG 3 cut(s) 647, 780, 807
BpuMI CCSGG 1 cut(s) 84
BsaHI GRCGYC 1 cut(s) 834
BsaI GGTCTC 1 cut(s) 23
BsaJI CCNNGG 2 cut(s) 424, 696
BsaWI WCCGGW 2 cut(s) 203, 572
BsaXI ACNNNNNCTCC 2 cut(s) 403, 433
Bsc4I CCNNNNNNNGG 1 cut(s) 797
Bse118I RCCGGY 2 cut(s) 113, 790
Bse1I ACTGG 4 cut(s) 93, 374, 443, 475
BseDI CCNNGG 2 cut(s) 424, 696
BseGI GGATG 2 cut(s) 19, 200
BseLI CCNNNNNNNGG 1 cut(s) 797
BseMII CTCAG 1 cut(s) 548
BseNI ACTGG 4 cut(s) 93, 374, 443, 475
BseRI GAGGAG 8 cut(s) 49, 67, 135, 138, 141, 144, 273, 844
BseXI GCAGC 2 cut(s) 116, 638
Bsh1285I CGRYCG 1 cut(s) 777
BshFI GGCC 2 cut(s) 429, 790
BshNI GGYRCC 1 cut(s) 692
BsiEI CGRYCG 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 539
BsiSI CCGG 5 cut(s) 83, 114, 204, 573, 791
BslFI GGGAC 1 cut(s) 99
BslI CCNNNNNNNGG 1 cut(s) 797
BsmAI GTCTC 4 cut(s) 23, 492, 665, 861
BsmBI CGTCTC 1 cut(s) 665
BsmFI GGGAC 1 cut(s) 99
BsnI GGCC 2 cut(s) 429, 790
Bso31I GGTCTC 1 cut(s) 23
Bsp119I TTCGAA 1 cut(s) 878
Bsp1286I GDGCHC 1 cut(s) 539
Bsp143I GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
BspACI CCGC 4 cut(s) 312, 321, 504, 564
BspANI GGCC 2 cut(s) 429, 790
BspCNI CTCAG 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 694
BspPI GGATC 4 cut(s) 99, 250, 402, 605
BspT104I TTCGAA 1 cut(s) 878
BspT107I GGYRCC 1 cut(s) 692
BspTNI GGTCTC 1 cut(s) 23
BsrFI RCCGGY 2 cut(s) 113, 790
BsrI ACTGG 4 cut(s) 93, 374, 443, 475
BssAI RCCGGY 2 cut(s) 113, 790
BssECI CCNNGG 2 cut(s) 424, 696
BssMI GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
BssNI GRCGYC 1 cut(s) 834
BssSI CACGAG 1 cut(s) 269
BssT1I CCWWGG 2 cut(s) 424, 696
Bst2BI CACGAG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 460, 546
Bst6I CTCTTC 2 cut(s) 67, 634
BstACI GRCGYC 1 cut(s) 834
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBI TTCGAA 1 cut(s) 878
BstC8I GCNNGC 1 cut(s) 792
BstDEI CTNAG 2 cut(s) 557, 588
BstF5I GGATG 2 cut(s) 19, 200
BstKTI GATC 9 cut(s) 73, 94, 169, 214, 245, 265, 410, 613, 888
BstMAI GTCTC 4 cut(s) 23, 492, 665, 861
BstMBI GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
BstMCI CGRYCG 1 cut(s) 777
BstMWI GCNNNNNNNGC 3 cut(s) 110, 318, 842
BstSCI CCNGG 1 cut(s) 82
BstV1I GCAGC 2 cut(s) 116, 638
BstX2I RGATCY 3 cut(s) 70, 407, 610
BstYI RGATCY 3 cut(s) 70, 407, 610
BsuRI GGCC 2 cut(s) 429, 790
BtsCI GGATG 2 cut(s) 19, 200
BtsIMutI CAGTG 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 792
Cfr10I RCCGGY 2 cut(s) 113, 790
CseI GACGC 1 cut(s) 842
Csp6I GTAC 1 cut(s) 755
CviAII CATG 1 cut(s) 349
CviQI GTAC 1 cut(s) 755
DdeI CTNAG 2 cut(s) 557, 588
DpnI GATC 9 cut(s) 72, 93, 168, 213, 244, 264, 409, 612, 887
DpnII GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
EaeI YGGCCR 1 cut(s) 788
Eam1104I CTCTTC 2 cut(s) 67, 634
EarI CTCTTC 2 cut(s) 67, 634
Eco130I CCWWGG 2 cut(s) 424, 696
Eco31I GGTCTC 1 cut(s) 23
EcoRI GAATTC 2 cut(s) 160, 874
EcoT14I CCWWGG 2 cut(s) 424, 696
ErhI CCWWGG 2 cut(s) 424, 696
Esp3I CGTCTC 1 cut(s) 665
FaeI CATG 1 cut(s) 352
FaiI YATR 7 cut(s) 111, 293, 350, 387, 389, 445, 738
FaqI GGGAC 1 cut(s) 99
FatI CATG 1 cut(s) 348
FauNDI CATATG 1 cut(s) 387
FbaI TGATCA 2 cut(s) 262, 885
Fnu4HI GCNGC 3 cut(s) 105, 322, 627
FokI GGATG 2 cut(s) 6, 187
Fsp4HI GCNGC 3 cut(s) 105, 322, 627
FspBI CTAG 3 cut(s) 405, 477, 623
GluI GCNGC 3 cut(s) 105, 322, 627
GsuI CTGGAG 1 cut(s) 270
HaeIII GGCC 2 cut(s) 429, 790
HapII CCGG 5 cut(s) 83, 114, 204, 573, 791
HgaI GACGC 1 cut(s) 842
Hin1I GRCGYC 1 cut(s) 834
Hin1II CATG 1 cut(s) 352
HinfI GANTC 5 cut(s) 373, 452, 485, 634, 880
HpaII CCGG 5 cut(s) 83, 114, 204, 573, 791
HphI GGTGA 4 cut(s) 218, 594, 721, 880
Hpy166II GTNNAC 1 cut(s) 656
Hpy188I TCNGA 4 cut(s) 67, 166, 451, 885
Hpy188III TCNNGA 4 cut(s) 269, 477, 664, 812
Hpy8I GTNNAC 1 cut(s) 656
Hpy99I CGWCG 1 cut(s) 550
HpyAV CCTTC 2 cut(s) 340, 758
HpyCH4III ACNGT 2 cut(s) 460, 546
HpyCH4IV ACGT 2 cut(s) 517, 658
HpyCH4V TGCA 2 cut(s) 104, 673
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 318, 842
HpyF3I CTNAG 2 cut(s) 557, 588
HpySE526I ACGT 2 cut(s) 517, 658
Hsp92I GRCGYC 1 cut(s) 834
Hsp92II CATG 1 cut(s) 352
KroI GCCGGC 1 cut(s) 790
KroNI GCCGGC 1 cut(s) 792
Ksp22I TGATCA 2 cut(s) 262, 885
Kzo9I GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
LmnI GCTCC 1 cut(s) 814
Lsp1109I GCAGC 2 cut(s) 116, 638
LweI GCATC 2 cut(s) 91, 832
MaeI CTAG 3 cut(s) 405, 477, 623
MaeII ACGT 2 cut(s) 517, 658
MaeIII GTNAC 3 cut(s) 463, 568, 709
MalI GATC 9 cut(s) 72, 93, 168, 213, 244, 264, 409, 612, 887
MboI GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
MboII GAAGA 5 cut(s) 54, 80, 221, 562, 651
MflI RGATCY 3 cut(s) 70, 407, 610
MhlI GDGCHC 1 cut(s) 539
MluCI AATT 5 cut(s) 34, 52, 160, 874, 892
MlyI GAGTC 1 cut(s) 494
MroNI GCCGGC 1 cut(s) 790
MroXI GAANNNNTTC 1 cut(s) 681
MseI TTAA 5 cut(s) 12, 255, 762, 828, 895
MspA1I CMGCKG 1 cut(s) 506
MspI CCGG 5 cut(s) 83, 114, 204, 573, 791
MspR9I CCNGG 1 cut(s) 84
MwoI GCNNNNNNNGC 3 cut(s) 110, 318, 842
NaeI GCCGGC 1 cut(s) 792
NciI CCSGG 1 cut(s) 84
NdeI CATATG 1 cut(s) 387
NdeII GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
NgoMIV GCCGGC 1 cut(s) 790
NlaIII CATG 1 cut(s) 352
NlaIV GGNNCC 1 cut(s) 694
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 2 cut(s) 568, 709
NspV TTCGAA 1 cut(s) 878
PdiI GCCGGC 1 cut(s) 792
PdmI GAANNNNTTC 1 cut(s) 681
PfeI GAWTC 4 cut(s) 373, 452, 634, 880
PkrI GCNGC 3 cut(s) 106, 323, 628
PleI GAGTC 1 cut(s) 493
PpsI GAGTC 1 cut(s) 493
Psp1406I AACGTT 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 694
PsuI RGATCY 3 cut(s) 70, 407, 610
RsaI GTAC 1 cut(s) 756
RsaNI GTAC 1 cut(s) 755
SaqAI TTAA 5 cut(s) 12, 255, 762, 828, 895
SatI GCNGC 3 cut(s) 105, 322, 627
Sau3AI GATC 9 cut(s) 70, 91, 166, 211, 242, 262, 407, 610, 885
SchI GAGTC 1 cut(s) 494
ScrFI CCNGG 1 cut(s) 84
SduI GDGCHC 1 cut(s) 539
SfaNI GCATC 2 cut(s) 91, 832
SfuI TTCGAA 1 cut(s) 878
SmlI CTYRAG 3 cut(s) 662, 795, 822
SmoI CTYRAG 3 cut(s) 662, 795, 822
Sse9I AATT 5 cut(s) 34, 52, 160, 874, 892
SsiI CCGC 4 cut(s) 312, 321, 504, 564
SspMI CTAG 3 cut(s) 405, 477, 623
StyD4I CCNGG 1 cut(s) 82
StyI CCWWGG 2 cut(s) 424, 696
TaaI ACNGT 2 cut(s) 460, 546
TaiI ACGT 2 cut(s) 520, 661
TaqI TCGA 4 cut(s) 143, 548, 637, 878
TaqII GACCGA 1 cut(s) 791
TasI AATT 5 cut(s) 34, 52, 160, 874, 892
TatI WGTACW 1 cut(s) 754
TauI GCSGC 1 cut(s) 324
TfiI GAWTC 4 cut(s) 373, 452, 634, 880
Tru1I TTAA 5 cut(s) 12, 255, 762, 828, 895
Tru9I TTAA 5 cut(s) 12, 255, 762, 828, 895
TscAI CASTG 1 cut(s) 475
TseFI GTSAC 2 cut(s) 568, 709
TseI GCWGC 2 cut(s) 104, 626
Tsp45I GTSAC 2 cut(s) 568, 709
TspDTI ATGAA 1 cut(s) 173
TspGWI ACGGA 1 cut(s) 255
TspRI CASTG 1 cut(s) 475
XapI RAATTY 3 cut(s) 52, 160, 874
XbaI TCTAGA 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 703
XmnI GAANNNNTTC 1 cut(s) 681
XspI CTAG 3 cut(s) 405, 477, 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.