AT1G69340

appr-1-p processing enzyme family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0006857
Physical Location & Seq
Forward (+)
26065698 .. 26069843
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69340.2

Sequence Viewer

Length: 1689 bp
ATGATGTATCAGACAATTCCCACGGCACCAACCATTCGTGGTGGAACTCCAACAGAGAGTGGTGATTACGTTGTGACTTTGGATCAGATTCCACGGTGGAGTGATGTTGAGCAGAGATCATCTTTGGAGGATGAGACAGGAGATCCAGAACATTCGAATCCGCGCTACGCAAACCCTTTGGCTTCGTCTTCCGAAGCAGGGAGCAGCGGGAATGGGATGGTATCTAAGTTTCCTGTTGATCACGAGATAAACTCTAGAATATACCTTTGGAGAGGGGAACCTTGGAACCTTGAGGTTGATGCTGTTGTAAATTCGACAAATGAGAACTTGGATGAGGCACATAGCAGTCCTGGTCTACATGTGGCTGCTGGACCTGGCCTTGCAGAGCAATGTGCTACATTGGGTGGATGCAGGACAGGGATGGCGAAAGTAACAAATGCCTATGATCTACCAGCGAGGAGGGTTATCCATACAGTAGGACCCAAGTATGCAGTAAAGTATCATACAGCTGCTGAGAATGCTCTAAGTCACTGCTATAGATCTTGTCTGGAACTTCTCATAGATAGTGGGCTACAAAGTATCGCTCTGGGCTGTATATACACAGAAGCCAAAAACTATCCCCGAGAACCAGCTGCTCATGTTGCCATAAGAACTGTTCGCCGCTTTCTAGAGAAGCAAAAGGATAAAATCAGTGCCGTTGTTTTCTGTACCACCACATCCTCTGATACGGAGATATACAAACGATTACTTCCACTTTACTTTCCTCGAGATGAACATGAGGAAGAGGTTGCCATATCAAAGCTCCCGGCTGATGTTGGAGATGAAAATGGTGAGACGGTTATAGACGAACGTAAAATCAGAATACAGGCGCTGCCAAATAAACCTCCACCCAGATCTTTTCCAACTCCACTCGAGCGCCCTTCTACTGATCTTACTTTGTTACGCAGGAACTCGAATCATCTTGATTCGTATTTGGATCCTGCCTTCATGTCCTTGATTAAGGATCCAGATGAAAGGCGCAAAGAACAATGGGAGAAGACTGCACAAGCACAAAGTGGATTCAATTTTGTTAAATTGCTAGGATTTGGTGATCTAGGAGGACCCCCTCTCTCTGCTGCAGAGGAATACTCACTTCATTCGCGATACCTAGCGAAAGCTAATTCAATCAACCTTTCCGAGATTGCAGAGATGAAAATTGTCTATCGTGGTGGTGTTGACACCGAGGGTCATCCTGTAATGGTTGTTGTAGGAGCTCACTTTTTGCTGCGATGTCTTGATCTGGAGCGTTTTGTACTTTATGTTATTAAGGAATTTGAACCTGTGATACAAAAGCCTTATTCGATTGTCTACTTTCATTCTGCAGCATCGTTACAAGTCCAACCAGATTTGGGATGGATGAAAAGATTAGAACAGATACTTGGTCGAAAACACCAGCGTAACCTTCAGGCAATCTATGTTCTTCATCCAACTTTCCACTTGAAGGCTACAATCTTAACGATGCAATTTTTCGTGGATAATGTGGTATGGAAGAAAGTTGTATATGCAGACCGACTTCTGCAGCTGTTCAAATACGTTCCTCGTGAGCAGCTGACAATCCCAGACTTTGTTTTCCAGCATGATCTTGAAGTTAACGGAGGAAAAGGTCTAATCGTTGACCCAAGAACAAAATACGTTTATCAACGGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2 Macro

Protein Analysis

562

Amino Acids

63.3

Weight (kDa)

6.24

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Macro PF01661 103 - 215 5e-28 Macro domain
CRAL_TRIO_2 PF13716 409 - 540 1.2e-31 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 25
AccI GTMKAC 2 cut(s) 355, 1347
AccII CGCG 2 cut(s) 163, 1141
AciI CCGC 3 cut(s) 161, 207, 661
AclWI GGATC 6 cut(s) 90, 137, 971, 984, 998, 1011
AcoI YGGCCR 1 cut(s) 1682
AcsI RAATTY 2 cut(s) 310, 1310
AcuI CTGAAG 1 cut(s) 1427
AdeI CACNNNGTG 1 cut(s) 1055
AfaI GTAC 2 cut(s) 709, 1293
AfiI CCNNNNNNNGG 3 cut(s) 198, 1388, 1480
AflIII ACRYGT 1 cut(s) 358
AgsI TTSAA 6 cut(s) 1063, 1164, 1316, 1480, 1567, 1625
AjnI CCWGG 2 cut(s) 349, 373
AjuI GAANNNNNNNTTGG 4 cut(s) 250, 282, 1401, 1433
AluBI AGCT 7 cut(s) 509, 632, 802, 1157, 1253, 1561, 1588
AluI AGCT 7 cut(s) 509, 632, 802, 1157, 1253, 1561, 1588
Alw21I GWGCWC 1 cut(s) 1255
Alw26I GTCTC 2 cut(s) 128, 827
AlwI GGATC 6 cut(s) 90, 137, 971, 984, 998, 1011
AlwNI CAGNNNCTG 2 cut(s) 512, 871
Ama87I CYCGRG 3 cut(s) 621, 765, 911
AoxI GGCC 2 cut(s) 376, 1682
ApoI RAATTY 2 cut(s) 310, 1310
AspLEI GCGC 4 cut(s) 165, 871, 918, 1020
AspS9I GGNCC 3 cut(s) 371, 479, 1100
AsuC2I CCSGG 1 cut(s) 806
AsuHPI GGTGA 3 cut(s) 74, 842, 1100
AsuII TTCGAA 1 cut(s) 155
AvaI CYCGRG 3 cut(s) 621, 765, 911
AvaII GGWCC 3 cut(s) 371, 479, 1100
BamHI GGATCC 2 cut(s) 976, 1003
BanI GGYRCC 1 cut(s) 25
BanII GRGCYC 1 cut(s) 1255
BauI CACGAG 2 cut(s) 242, 1578
BbsI GAAGAC 2 cut(s) 180, 1043
Bbv12I GWGCWC 1 cut(s) 1255
BccI CCATC 3 cut(s) 211, 415, 1386
BceAI ACGGC 2 cut(s) 39, 680
BciT130I CCWGG 2 cut(s) 351, 375
BclI TGATCA 1 cut(s) 238
BcnI CCSGG 1 cut(s) 806
BcoDI GTCTC 2 cut(s) 128, 827
BfaI CTAG 5 cut(s) 255, 668, 1079, 1094, 1148
BfmI CTRYAG 4 cut(s) 535, 1116, 1359, 1556
BfoI RGCGCY 2 cut(s) 872, 919
BglII AGATCT 2 cut(s) 539, 893
Bme1390I CCNGG 3 cut(s) 351, 375, 806
Bme18I GGWCC 3 cut(s) 371, 479, 1100
BmeT110I CYCGRG 3 cut(s) 621, 765, 911
BmgT120I GGNCC 3 cut(s) 371, 479, 1100
BmiI GGNNCC 7 cut(s) 27, 279, 287, 481, 978, 1005, 1102
BmrFI CCNGG 3 cut(s) 351, 375, 806
BmsI GCATC 4 cut(s) 289, 398, 1373, 1488
BpiI GAAGAC 2 cut(s) 180, 1043
BplI GAGNNNNNCTC 4 cut(s) 236, 268, 1112, 1144
BpmI CTGGAG 1 cut(s) 1301
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 806
BsaJI CCNNGG 4 cut(s) 21, 92, 281, 1221
Bsc4I CCNNNNNNNGG 3 cut(s) 198, 1388, 1480
Bse3DI GCAATG 1 cut(s) 395
BseBI CCWGG 2 cut(s) 351, 375
BseDI CCNNGG 4 cut(s) 21, 92, 281, 1221
BseLI CCNNNNNNNGG 3 cut(s) 198, 1388, 1480
BseMI GCAATG 1 cut(s) 395
BseMII CTCAG 1 cut(s) 504
BseRI GAGGAG 1 cut(s) 472
BsgI GTGCAG 1 cut(s) 1026
Bsh1236I CGCG 2 cut(s) 163, 1141
BshFI GGCC 2 cut(s) 378, 1684
BshNI GGYRCC 1 cut(s) 25
BsiHKAI GWGCWC 1 cut(s) 1255
BsiHKCI CYCGRG 3 cut(s) 621, 765, 911
BsiSI CCGG 1 cut(s) 806
BslI CCNNNNNNNGG 3 cut(s) 198, 1388, 1480
BsmAI GTCTC 2 cut(s) 128, 827
BsmBI CGTCTC 1 cut(s) 827
BsmI GAATGC 1 cut(s) 523
BsnI GGCC 2 cut(s) 378, 1684
BsoBI CYCGRG 3 cut(s) 621, 765, 911
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 1255
Bsp68I TCGCGA 1 cut(s) 1141
BspACI CCGC 3 cut(s) 161, 207, 661
BspANI GGCC 2 cut(s) 378, 1684
BspCNI CTCAG 1 cut(s) 505
BspFNI CGCG 2 cut(s) 163, 1141
BspLI GGNNCC 7 cut(s) 27, 279, 287, 481, 978, 1005, 1102
BspMAI CTGCAG 3 cut(s) 1120, 1363, 1560
BspPI GGATC 6 cut(s) 90, 137, 971, 984, 998, 1011
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 395
BssECI CCNNGG 4 cut(s) 21, 92, 281, 1221
BssSI CACGAG 2 cut(s) 242, 1578
BssT1I CCWWGG 1 cut(s) 281
Bst2BI CACGAG 2 cut(s) 242, 1578
Bst2UI CCWGG 2 cut(s) 351, 375
Bst4CI ACNGT 4 cut(s) 96, 475, 655, 838
Bst6I CTCTTC 1 cut(s) 777
BstBI TTCGAA 1 cut(s) 155
BstDEI CTNAG 3 cut(s) 225, 513, 524
BstDSI CCRYGG 2 cut(s) 21, 92
BstFNI CGCG 2 cut(s) 163, 1141
BstH2I RGCGCY 2 cut(s) 872, 919
BstHHI GCGC 4 cut(s) 165, 871, 918, 1020
BstMAI GTCTC 2 cut(s) 128, 827
BstMWI GCNNNNNNNGC 2 cut(s) 518, 641
BstNI CCWGG 2 cut(s) 351, 375
BstNSI RCATGY 1 cut(s) 362
BstSCI CCNGG 3 cut(s) 349, 373, 804
BstSFI CTRYAG 4 cut(s) 535, 1116, 1359, 1556
BstUI CGCG 2 cut(s) 163, 1141
BstV2I GAAGAC 2 cut(s) 180, 1043
BstX2I RGATCY 5 cut(s) 142, 539, 893, 976, 1003
BstYI RGATCY 5 cut(s) 142, 539, 893, 976, 1003
BsuRI GGCC 2 cut(s) 378, 1684
BtgI CCRYGG 2 cut(s) 21, 92
BtgZI GCGATG 1 cut(s) 1282
BtsI GCAGTG 1 cut(s) 529
BtsIMutI CAGTG 2 cut(s) 529, 697
BtuMI TCGCGA 1 cut(s) 1141
CaiI CAGNNNCTG 2 cut(s) 512, 871
CfoI GCGC 4 cut(s) 165, 871, 918, 1020
Cfr13I GGNCC 3 cut(s) 371, 479, 1100
Csp6I GTAC 2 cut(s) 708, 1292
CviAII CATG 6 cut(s) 359, 638, 776, 988, 1616, 1686
CviQI GTAC 2 cut(s) 708, 1292
DdeI CTNAG 3 cut(s) 225, 513, 524
DraIII CACNNNGTG 1 cut(s) 1055
EaeI YGGCCR 1 cut(s) 1682
Eam1104I CTCTTC 1 cut(s) 777
EarI CTCTTC 1 cut(s) 777
Ecl136II GAGCTC 1 cut(s) 1253
Eco130I CCWWGG 1 cut(s) 281
Eco24I GRGCYC 1 cut(s) 1255
Eco47I GGWCC 3 cut(s) 371, 479, 1100
Eco53kI GAGCTC 1 cut(s) 1253
Eco57I CTGAAG 1 cut(s) 1427
Eco88I CYCGRG 3 cut(s) 621, 765, 911
EcoICRI GAGCTC 1 cut(s) 1253
EcoO109I RGGNCCY 2 cut(s) 479, 1100
EcoRII CCWGG 2 cut(s) 349, 373
EcoT14I CCWWGG 1 cut(s) 281
EcoT38I GRGCYC 1 cut(s) 1255
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 827
FaeI CATG 6 cut(s) 362, 641, 779, 991, 1619, 1689
FatI CATG 6 cut(s) 358, 637, 775, 987, 1615, 1685
FauI CCCGC 1 cut(s) 200
FbaI TGATCA 1 cut(s) 238
FblI GTMKAC 2 cut(s) 355, 1347
FriOI GRGCYC 1 cut(s) 1255
FspBI CTAG 5 cut(s) 255, 668, 1079, 1094, 1148
GlaI GCGC 4 cut(s) 164, 870, 917, 1019
GsuI CTGGAG 1 cut(s) 1301
HaeII RGCGCY 2 cut(s) 872, 919
HaeIII GGCC 2 cut(s) 378, 1684
HapII CCGG 1 cut(s) 806
HhaI GCGC 4 cut(s) 165, 871, 918, 1020
Hin1II CATG 6 cut(s) 362, 641, 779, 991, 1619, 1689
Hin6I GCGC 4 cut(s) 163, 869, 916, 1018
HinP1I GCGC 4 cut(s) 163, 869, 916, 1018
HincII GTYRAC 3 cut(s) 1216, 1630, 1654
HindII GTYRAC 3 cut(s) 1216, 1630, 1654
HinfI GANTC 5 cut(s) 88, 157, 955, 965, 1059
HpaI GTTAAC 1 cut(s) 1630
HpaII CCGG 1 cut(s) 806
HphI GGTGA 3 cut(s) 74, 842, 1100
Hpy166II GTNNAC 5 cut(s) 356, 1216, 1348, 1630, 1654
Hpy188I TCNGA 6 cut(s) 12, 87, 193, 724, 860, 1177
Hpy8I GTNNAC 5 cut(s) 356, 1216, 1348, 1630, 1654
HpyAV CCTTC 4 cut(s) 930, 994, 1451, 1474
HpyCH4III ACNGT 4 cut(s) 96, 475, 655, 838
HpyCH4IV ACGT 4 cut(s) 69, 850, 1572, 1671
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 641
HpyF3I CTNAG 3 cut(s) 225, 513, 524
HpySE526I ACGT 4 cut(s) 69, 850, 1572, 1671
Hsp92II CATG 6 cut(s) 362, 641, 779, 991, 1619, 1689
HspAI GCGC 4 cut(s) 163, 869, 916, 1018
Ksp22I TGATCA 1 cut(s) 238
KspAI GTTAAC 1 cut(s) 1630
LmnI GCTCC 4 cut(s) 201, 807, 1250, 1282
LweI GCATC 4 cut(s) 289, 398, 1373, 1488
MaeI CTAG 5 cut(s) 255, 668, 1079, 1094, 1148
MaeII ACGT 4 cut(s) 69, 850, 1572, 1671
MaeIII GTNAC 6 cut(s) 73, 430, 527, 939, 1368, 1436
MboII GAAGA 5 cut(s) 180, 794, 1048, 1451, 1540
MflI RGATCY 5 cut(s) 142, 539, 893, 976, 1003
MhlI GDGCHC 1 cut(s) 1255
MluCI AATT 8 cut(s) 15, 310, 1063, 1073, 1159, 1194, 1310, 1502
MmeI TCCRAC 5 cut(s) 74, 796, 926, 1402, 1490
MseI TTAA 5 cut(s) 999, 1071, 1305, 1493, 1629
MspA1I CMGCKG 5 cut(s) 207, 509, 632, 1561, 1588
MspI CCGG 1 cut(s) 806
MspR9I CCNGG 3 cut(s) 351, 375, 806
Mva1269I GAATGC 1 cut(s) 523
MvaI CCWGG 2 cut(s) 351, 375
MvnI CGCG 2 cut(s) 163, 1141
MwoI GCNNNNNNNGC 2 cut(s) 518, 641
NciI CCSGG 1 cut(s) 806
NlaIII CATG 6 cut(s) 362, 641, 779, 991, 1619, 1689
NlaIV GGNNCC 7 cut(s) 27, 279, 287, 481, 978, 1005, 1102
NmuCI GTSAC 2 cut(s) 73, 527
NruI TCGCGA 1 cut(s) 1141
NspI RCATGY 1 cut(s) 362
NspV TTCGAA 1 cut(s) 155
PaeR7I CTCGAG 2 cut(s) 765, 911
PciI ACATGT 1 cut(s) 358
PctI GAATGC 1 cut(s) 523
PfeI GAWTC 5 cut(s) 88, 157, 955, 965, 1059
PpuMI RGGWCCY 2 cut(s) 479, 1100
PscI ACATGT 1 cut(s) 358
Psp124BI GAGCTC 1 cut(s) 1255
Psp5II RGGWCCY 2 cut(s) 479, 1100
Psp6I CCWGG 2 cut(s) 349, 373
PspGI CCWGG 2 cut(s) 349, 373
PspN4I GGNNCC 7 cut(s) 27, 279, 287, 481, 978, 1005, 1102
PspPI GGNCC 3 cut(s) 371, 479, 1100
PspPPI RGGWCCY 2 cut(s) 479, 1100
PspXI VCTCGAGB 1 cut(s) 911
PsrI GAACNNNNNNTAC 2 cut(s) 1308, 1340
PstI CTGCAG 3 cut(s) 1120, 1363, 1560
PstNI CAGNNNCTG 2 cut(s) 512, 871
PsuI RGATCY 5 cut(s) 142, 539, 893, 976, 1003
PvuII CAGCTG 4 cut(s) 509, 632, 1561, 1588
RruI TCGCGA 1 cut(s) 1141
RsaI GTAC 2 cut(s) 709, 1293
RsaNI GTAC 2 cut(s) 708, 1292
SacI GAGCTC 1 cut(s) 1255
SaqAI TTAA 5 cut(s) 999, 1071, 1305, 1493, 1629
Sau96I GGNCC 3 cut(s) 371, 479, 1100
ScrFI CCNGG 3 cut(s) 351, 375, 806
SduI GDGCHC 1 cut(s) 1255
SfaNI GCATC 4 cut(s) 289, 398, 1373, 1488
SfcI CTRYAG 4 cut(s) 535, 1116, 1359, 1556
Sfr274I CTCGAG 2 cut(s) 765, 911
SfuI TTCGAA 1 cut(s) 155
SinI GGWCC 3 cut(s) 371, 479, 1100
SlaI CTCGAG 2 cut(s) 765, 911
SmlI CTYRAG 3 cut(s) 290, 765, 911
SmoI CTYRAG 3 cut(s) 290, 765, 911
Sse9I AATT 8 cut(s) 15, 310, 1063, 1073, 1159, 1194, 1310, 1502
SsiI CCGC 3 cut(s) 161, 207, 661
SspMI CTAG 5 cut(s) 255, 668, 1079, 1094, 1148
SstI GAGCTC 1 cut(s) 1255
StyD4I CCNGG 3 cut(s) 349, 373, 804
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 4 cut(s) 96, 475, 655, 838
TaiI ACGT 4 cut(s) 72, 853, 1575, 1674
TaqI TCGA 7 cut(s) 155, 314, 766, 912, 953, 1340, 1423
TaqII GACCGA 1 cut(s) 1563
TasI AATT 8 cut(s) 15, 310, 1063, 1073, 1159, 1194, 1310, 1502
TatI WGTACW 1 cut(s) 1291
TauI GCSGC 1 cut(s) 663
TfiI GAWTC 5 cut(s) 88, 157, 955, 965, 1059
Tru1I TTAA 5 cut(s) 999, 1071, 1305, 1493, 1629
Tru9I TTAA 5 cut(s) 999, 1071, 1305, 1493, 1629
TscAI CASTG 2 cut(s) 536, 697
TseFI GTSAC 2 cut(s) 73, 527
Tsp45I GTSAC 2 cut(s) 73, 527
TspDTI ATGAA 9 cut(s) 786, 837, 976, 1026, 1124, 1205, 1343, 1412, 1451
TspGWI ACGGA 2 cut(s) 743, 1647
TspRI CASTG 2 cut(s) 536, 697
VpaK11BI GGWCC 3 cut(s) 371, 479, 1100
XapI RAATTY 2 cut(s) 310, 1310
XbaI TCTAGA 2 cut(s) 254, 667
XceI RCATGY 1 cut(s) 362
XcmI CCANNNNNNNNNTGG 1 cut(s) 1389
XhoI CTCGAG 2 cut(s) 765, 911
XmiI GTMKAC 2 cut(s) 355, 1347
XspI CTAG 5 cut(s) 255, 668, 1079, 1094, 1148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.