Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0006857

Physical Location & Seq

Genomic Coordinates Forward (+)

4556249 .. 4561237

Gene Structure 4989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1064800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1689 bp

ATGTACCGGACCGTGGCTACAGCCACAACTGCAACCAGGGGCGGATCTCCAACTGATAGTGGGGATTGTGTTGTCACTTTGGATCAAGTTCCACGGTGGAGCAATTCAGAGCATCGTTCTTCCTTGGAATATGACAATGAAGATCCTTCGTTTTCAAATTCGTTTTTTCCTGATCCTTTAACTTCCCAATCTGGGGGAGAGAGCAGTAGCAATGGGATAGTCTCAAGATTTCCAGTTGACCATGAAATTAATTCAAAGATATATCTATGGAGAGGGAACCCATGGAATCTCGAAGTTGATGCGGTAGTGAATTCTACAAATGAGAACATGGATGAAGCGCATTGTAGTCCTGGTCTGCATGCTGCGGCTGGACCTGGTCTTGCGGAAGAATGTGCATCACTGGGTGGATGTAGAACAGGGATGGCAAAGGTTACTAAAGCGTATGACCTTCCAGCTAGGAGGGTTATCCATACCGTCGGTCCCAAGTATGCAGTGAAATACCATACTGCTGCAGAAAATGCTCTGAGCCATTGTTATCGTTCTTGCCTTGAACTTCTCATTGAAAATGGGCTGCAAAGCATTGCAATGGGCTGTATATATACAGAAGCTAAGAACTACCCTCGTGAGCCAGCTGCCCATGTTGCTATAAGGACTGTACGACGTTTTCTGGAAAAGCAGAAAGATAAAATCGCAGCTGTTGTTTTTTGTACTACCACATCAATGGATACAGAGATATACAAAAGATTGCTTCCGCTTTACTTTCCCCGAGATAAACTTGAAGAGGAGATTGCCTTGTCGAAGCTTCCTGCAGATGTAGGTGATGAAAATGGCGAGACAATTATAGATGAGCGCAAAATCAGAATAAAGCCTTTGCCCAAGAAGAATATTCCAAAGCCTCCCCAGGCTCCAGTTGAGCTTCCTGTGAGTGATGTTGGCTTGGTACAAAGGAACTCACCATATTTGGATTCATATTTGGATCCTGCCTTCATGTCCTTAATAAAAGATCCAGATCAGAGACGCAAGGAACAATGGGAGAAAACTGCTCAAGCACAAAGTGGATGGAATTGTGCTAAAATTCTTGGGTTTGGCGACCTTGGTGGACCTCCATTGTCTGCTGCAGAAGAATACTCGCTTCATTCAAGATACCTTGCTAAAGCAAATTCTCTTAATCTTTCAGAACTTGCAGAAATGAAAATTGTTTACCGAGGTGGGGTTGACAGCGAGGGTCGTCCTGTAATGGTGGTTGTGGGGGCACATTTTCTTTTGCGATGTCTTGATCTAGAACGATTTGTACACTATGTTATTAAGGAGTTTGAACCCTTAATACAAAAGCCTTATACTATTGTATACTTCCACTCAGCAGCATCTTTACAGCTCCAGCCAGACTTGGGGTGGATGAAAAGGGTACAACAGATACTTGGTCGTAAACACCAACGTAATCTGCATGCAATATACGTCCTTCACCCGACATTTGGGCTGAAGGCTGCAATATTTGCCCTGCAACTGTTCGTGGACAATGTGGTCTGGAAGAAAGTGGTATATGTAGATAGATTACTGCAGCTCTTTCGATATGTTCCTCGTGAGCAGTTGACCATCCCCGACTTTGTGTTCCAGCACGATTTGGAAGTGAACGGTGGGAAGGGTCTTATCGTGGACCCGAGAACCAAGTATGTGTATCATCGACCGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CRAL_TRIO_2 Macro

Protein Analysis

563

Amino Acids

63.14

Weight (kDa)

7.26

Isoelectric Point (pI)

43.19

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Macro	PF01661	103 - 215	1.7e-30	Macro domain
CRAL_TRIO_2	PF13716	409 - 540	1.1e-33	Divergent CRAL/TRIO domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006857)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G69340 AT1G69340
fragaria_vesca	FvH4_4g30490 FvH4_4g30490 FvH4_4g30490 FvH4_4g30490
malus_domestica	MD13G1066000.v1.1 MD16G1064800.v1.1
prunus_persica	Prupe.1G282100_v2.0.a1 Prupe.1G282100_v2.0.a1
pyrus_communis	pycom13g05820 pycom16g05660
rosa_chinensis	RchiOBHm_Chr4g0438861
rosa_laevigata	RLG00000006324
rosa_multiflora	Rmu_sc0002505.1_g000021
rosa_roxburghii	Rroxscaffold_5G00379880 Rroxscaffold_7G00178430
rosa_rugosa	Rorug04G0313600 Rorug04G0313700 Rorug04G0313800
rosa_samantha	Rh4AG364600 Rh4BG375700 Rh4CG389800 Rh4DG370400
rosa_wichuraiana	Rw0G007270 Rw0G016820

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1520
AccI	GTMKAC	1 cut(s)	1347
AciI	CCGC	5 cut(s)	42, 302, 365, 383, 752
AclWI	GGATC	7 cut(s)	52, 90, 137, 167, 971, 984, 998
AcsI	RAATTY	4 cut(s)	157, 310, 1074, 1159
AcuI	CTGAAG	1 cut(s)	1499
AdeI	CACNNNGTG	2 cut(s)	404, 1055
AfaI	GTAC	6 cut(s)	5, 657, 709, 942, 1293, 1407
AfiI	CCNNNNNNNGG	6 cut(s)	13, 192, 193, 1210, 1388, 1472
AgsI	TTSAA	7 cut(s)	156, 255, 551, 563, 779, 1140, 1316
AjnI	CCWGG	4 cut(s)	35, 349, 373, 900
AluBI	AGCT	8 cut(s)	455, 608, 632, 695, 802, 916, 1375, 1561
AluI	AGCT	8 cut(s)	455, 608, 632, 695, 802, 916, 1375, 1561
Alw26I	GTCTC	3 cut(s)	226, 827, 1009
AlwI	GGATC	7 cut(s)	52, 90, 137, 167, 971, 984, 998
Ama87I	CYCGRG	2 cut(s)	765, 1657
ApeKI	GCWGC	9 cut(s)	362, 509, 571, 632, 692, 1115, 1361, 1484, 1558
ApoI	RAATTY	4 cut(s)	157, 310, 1074, 1159
AseI	ATTAAT	1 cut(s)	249
AspLEI	GCGC	2 cut(s)	340, 852
AspS9I	GGNCC	5 cut(s)	9, 371, 479, 1100, 1654
AsuHPI	GGTGA	3 cut(s)	830, 945, 1454
AvaI	CYCGRG	2 cut(s)	765, 1657
AvaII	GGWCC	5 cut(s)	9, 371, 479, 1100, 1654
BaeGI	GKGCMC	1 cut(s)	1255
BamHI	GGATCC	1 cut(s)	976
BauI	CACGAG	2 cut(s)	621, 1578
BbvI	GCAGC	9 cut(s)	349, 496, 558, 619, 704, 1102, 1373, 1471, 1570
BccI	CCATC	3 cut(s)	415, 1053, 1601
BcgI	CGANNNNNNTGC	4 cut(s)	281, 315, 1547, 1581
BciT130I	CCWGG	4 cut(s)	37, 351, 375, 902
BciVI	GTATCC	1 cut(s)	718
BcoDI	GTCTC	3 cut(s)	226, 827, 1009
BfaI	CTAG	2 cut(s)	456, 1280
BfmI	CTRYAG	5 cut(s)	18, 510, 807, 1116, 1556
BfuI	GTATCC	1 cut(s)	718
Bme1390I	CCNGG	4 cut(s)	37, 351, 375, 902
Bme18I	GGWCC	5 cut(s)	9, 371, 479, 1100, 1654
BmeT110I	CYCGRG	2 cut(s)	765, 1657
BmgT120I	GGNCC	5 cut(s)	9, 371, 479, 1100, 1654
BmiI	GGNNCC	5 cut(s)	278, 481, 906, 978, 1656
BmrFI	CCNGG	4 cut(s)	37, 351, 375, 902
BmrI	ACTGGG	1 cut(s)	410
BmsI	GCATC	4 cut(s)	121, 289, 404, 1373
BmuI	ACTGGG	1 cut(s)	410
BpmI	CTGGAG	2 cut(s)	891, 1361
BpuEI	CTTGAG	2 cut(s)	208, 1029
BsaBI	GATNNNNATC	1 cut(s)	1008
BsaJI	CCNNGG	8 cut(s)	12, 36, 92, 123, 281, 900, 1093, 1204
BsaWI	WCCGGW	1 cut(s)	6
Bsc4I	CCNNNNNNNGG	6 cut(s)	13, 192, 193, 1210, 1388, 1472
Bse1I	ACTGG	3 cut(s)	233, 405, 908
Bse3DI	GCAATG	3 cut(s)	217, 579, 591
Bse8I	GATNNNNATC	1 cut(s)	1008
BseBI	CCWGG	4 cut(s)	37, 351, 375, 902
BseDI	CCNNGG	8 cut(s)	12, 36, 92, 123, 281, 900, 1093, 1204
BseGI	GGATG	6 cut(s)	337, 413, 426, 1064, 1401, 1593
BseJI	GATNNNNATC	1 cut(s)	1008
BseLI	CCNNNNNNNGG	6 cut(s)	13, 192, 193, 1210, 1388, 1472
BseMI	GCAATG	3 cut(s)	217, 579, 591
BseMII	CTCAG	2 cut(s)	515, 1371
BseNI	ACTGG	3 cut(s)	233, 405, 908
BseRI	GAGGAG	1 cut(s)	797
BseSI	GKGCMC	1 cut(s)	1255
BseXI	GCAGC	9 cut(s)	349, 496, 558, 619, 704, 1102, 1373, 1471, 1570
Bsh1285I	CGRYCG	1 cut(s)	1685
BsiEI	CGRYCG	1 cut(s)	1685
BsiHKCI	CYCGRG	2 cut(s)	765, 1657
BsiSI	CCGG	1 cut(s)	7
BslFI	GGGAC	1 cut(s)	465
BslI	CCNNNNNNNGG	6 cut(s)	13, 192, 193, 1210, 1388, 1472
BsmAI	GTCTC	3 cut(s)	226, 827, 1009
BsmBI	CGTCTC	1 cut(s)	1009
BsmFI	GGGAC	1 cut(s)	465
BsoBI	CYCGRG	2 cut(s)	765, 1657
Bsp1286I	GDGCHC	1 cut(s)	1255
Bsp1407I	TGTACA	1 cut(s)	1291
Bsp143I	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
Bsp19I	CCATGG	1 cut(s)	281
BspACI	CCGC	5 cut(s)	42, 302, 365, 383, 752
BspCNI	CTCAG	2 cut(s)	516, 1370
BspLI	GGNNCC	5 cut(s)	278, 481, 906, 978, 1656
BspMAI	CTGCAG	4 cut(s)	514, 811, 1120, 1560
BspPI	GGATC	7 cut(s)	52, 90, 137, 167, 971, 984, 998
BsrDI	GCAATG	3 cut(s)	217, 579, 591
BsrGI	TGTACA	1 cut(s)	1291
BsrI	ACTGG	3 cut(s)	233, 405, 908
BssECI	CCNNGG	8 cut(s)	12, 36, 92, 123, 281, 900, 1093, 1204
BssMI	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
BssNAI	GTATAC	1 cut(s)	1348
BssSI	CACGAG	2 cut(s)	621, 1578
BssT1I	CCWWGG	3 cut(s)	123, 281, 1093
Bst1107I	GTATAC	1 cut(s)	1348
Bst2BI	CACGAG	2 cut(s)	621, 1578
Bst2UI	CCWGG	4 cut(s)	37, 351, 375, 902
Bst4CI	ACNGT	7 cut(s)	13, 96, 475, 655, 1506, 1634, 1686
Bst6I	CTCTTC	1 cut(s)	774
BstAPI	GCANNNNNTGC	2 cut(s)	518, 1493
BstAUI	TGTACA	1 cut(s)	1291
BstC8I	GCNNGC	3 cut(s)	360, 630, 1446
BstDEI	CTNAG	3 cut(s)	524, 609, 1357
BstDSI	CCRYGG	3 cut(s)	12, 92, 281
BstF5I	GGATG	6 cut(s)	337, 413, 426, 1064, 1401, 1593
BstHHI	GCGC	2 cut(s)	340, 852
BstKTI	GATC	8 cut(s)	47, 85, 145, 175, 979, 1006, 1012, 1279
BstMAI	GTCTC	3 cut(s)	226, 827, 1009
BstMBI	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
BstMCI	CGRYCG	1 cut(s)	1685
BstMWI	GCNNNNNNNGC	4 cut(s)	29, 518, 641, 1493
BstNI	CCWGG	4 cut(s)	37, 351, 375, 902
BstNSI	RCATGY	2 cut(s)	362, 1448
BstSCI	CCNGG	4 cut(s)	35, 349, 373, 900
BstSFI	CTRYAG	5 cut(s)	18, 510, 807, 1116, 1556
BstSLI	GKGCMC	1 cut(s)	1255
BstV1I	GCAGC	9 cut(s)	349, 496, 558, 619, 704, 1102, 1373, 1471, 1570
BstX2I	RGATCY	4 cut(s)	44, 142, 976, 1003
BstXI	CCANNNNNNTGG	1 cut(s)	721
BstYI	RGATCY	4 cut(s)	44, 142, 976, 1003
BstZ17I	GTATAC	1 cut(s)	1348
BsuI	GTATCC	1 cut(s)	718
BtgI	CCRYGG	3 cut(s)	12, 92, 281
BtgZI	GCGATG	1 cut(s)	1282
BtsCI	GGATG	6 cut(s)	337, 413, 426, 1064, 1401, 1593
BtsI	GCAGTG	1 cut(s)	498
BtsIMutI	CAGTG	2 cut(s)	398, 498
Cac8I	GCNNGC	3 cut(s)	360, 630, 1446
CfoI	GCGC	2 cut(s)	340, 852
Cfr13I	GGNCC	5 cut(s)	9, 371, 479, 1100, 1654
CpoI	CGGWCCG	1 cut(s)	9
CseI	GACGC	1 cut(s)	1026
CsiI	ACCWGGT	1 cut(s)	373
Csp6I	GTAC	6 cut(s)	4, 656, 708, 941, 1292, 1406
CspI	CGGWCCG	1 cut(s)	9
CviAII	CATG	7 cut(s)	242, 282, 328, 359, 638, 988, 1445
CviQI	GTAC	6 cut(s)	4, 656, 708, 941, 1292, 1406
DdeI	CTNAG	3 cut(s)	524, 609, 1357
DpnI	GATC	8 cut(s)	46, 84, 144, 174, 978, 1005, 1011, 1278
DpnII	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
DraIII	CACNNNGTG	2 cut(s)	404, 1055
DrdI	GACNNNNNNGTC	1 cut(s)	1520
DseDI	GACNNNNNNGTC	1 cut(s)	1520
Eam1104I	CTCTTC	1 cut(s)	774
EarI	CTCTTC	1 cut(s)	774
EciI	GGCGGA	1 cut(s)	57
Eco130I	CCWWGG	3 cut(s)	123, 281, 1093
Eco47I	GGWCC	5 cut(s)	9, 371, 479, 1100, 1654
Eco57I	CTGAAG	1 cut(s)	1499
Eco88I	CYCGRG	2 cut(s)	765, 1657
EcoRI	GAATTC	1 cut(s)	310
EcoRII	CCWGG	4 cut(s)	35, 349, 373, 900
EcoT14I	CCWWGG	3 cut(s)	123, 281, 1093
ErhI	CCWWGG	3 cut(s)	123, 281, 1093
Esp3I	CGTCTC	1 cut(s)	1009
FaeI	CATG	7 cut(s)	245, 285, 331, 362, 641, 991, 1448
FaqI	GGGAC	1 cut(s)	465
FatI	CATG	7 cut(s)	241, 281, 327, 358, 637, 987, 1444
FblI	GTMKAC	1 cut(s)	1347
FokI	GGATG	6 cut(s)	344, 420, 433, 1071, 1408, 1580
FspBI	CTAG	2 cut(s)	456, 1280
GlaI	GCGC	2 cut(s)	339, 851
GsuI	CTGGAG	2 cut(s)	891, 1361
HapII	CCGG	1 cut(s)	7
HgaI	GACGC	1 cut(s)	1026
HhaI	GCGC	2 cut(s)	340, 852
Hin1II	CATG	7 cut(s)	245, 285, 331, 362, 641, 991, 1448
Hin6I	GCGC	2 cut(s)	338, 850
HinP1I	GCGC	2 cut(s)	338, 850
HincII	GTYRAC	3 cut(s)	238, 1216, 1590
HindII	GTYRAC	3 cut(s)	238, 1216, 1590
HindIII	AAGCTT	1 cut(s)	800
HinfI	GANTC	2 cut(s)	286, 965
HpaII	CCGG	1 cut(s)	7
HphI	GGTGA	3 cut(s)	830, 945, 1454
Hpy188I	TCNGA	5 cut(s)	109, 525, 860, 1014, 1177
Hpy99I	CGWCG	2 cut(s)	479, 663
HpyAV	CCTTC	6 cut(s)	156, 458, 994, 1469, 1474, 1633
HpyCH4III	ACNGT	7 cut(s)	13, 96, 475, 655, 1506, 1634, 1686
HpyCH4IV	ACGT	3 cut(s)	661, 1435, 1455
HpyF10VI	GCNNNNNNNGC	4 cut(s)	29, 518, 641, 1493
HpyF3I	CTNAG	3 cut(s)	524, 609, 1357
HpySE526I	ACGT	3 cut(s)	661, 1435, 1455
Hsp92II	CATG	7 cut(s)	245, 285, 331, 362, 641, 991, 1448
HspAI	GCGC	2 cut(s)	338, 850
Kzo9I	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
LmnI	GCTCC	3 cut(s)	99, 910, 1380
Lsp1109I	GCAGC	9 cut(s)	349, 496, 558, 619, 704, 1102, 1373, 1471, 1570
LweI	GCATC	4 cut(s)	121, 289, 404, 1373
MabI	ACCWGGT	1 cut(s)	373
MaeI	CTAG	2 cut(s)	456, 1280
MaeII	ACGT	3 cut(s)	661, 1435, 1455
MaeIII	GTNAC	2 cut(s)	73, 430
MalI	GATC	8 cut(s)	46, 84, 144, 174, 978, 1005, 1011, 1278
MboI	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
MboII	GAAGA	7 cut(s)	111, 152, 398, 791, 892, 1133, 1540
MflI	RGATCY	4 cut(s)	44, 142, 976, 1003
MhlI	GDGCHC	1 cut(s)	1255
MmeI	TCCRAC	1 cut(s)	74
MnlI	CCTC	9 cut(s)	266, 453, 630, 775, 906, 1113, 1199, 1216, 1587
MseI	TTAA	6 cut(s)	179, 249, 995, 1167, 1305, 1322
MslI	CAYNNNNRTG	2 cut(s)	584, 719
MspA1I	CMGCKG	2 cut(s)	632, 695
MspI	CCGG	1 cut(s)	7
MspR9I	CCNGG	4 cut(s)	37, 351, 375, 902
MvaI	CCWGG	4 cut(s)	37, 351, 375, 902
MwoI	GCNNNNNNNGC	4 cut(s)	29, 518, 641, 1493
NcoI	CCATGG	1 cut(s)	281
NdeII	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
NlaIII	CATG	7 cut(s)	245, 285, 331, 362, 641, 991, 1448
NlaIV	GGNNCC	5 cut(s)	278, 481, 906, 978, 1656
NmuCI	GTSAC	1 cut(s)	73
NspI	RCATGY	2 cut(s)	362, 1448
PaeI	GCATGC	2 cut(s)	362, 1448
PfeI	GAWTC	2 cut(s)	286, 965
PflFI	GACNNNGTC	1 cut(s)	375
PshBI	ATTAAT	1 cut(s)	249
Psp6I	CCWGG	4 cut(s)	35, 349, 373, 900
PspGI	CCWGG	4 cut(s)	35, 349, 373, 900
PspN4I	GGNNCC	5 cut(s)	278, 481, 906, 978, 1656
PspPI	GGNCC	5 cut(s)	9, 371, 479, 1100, 1654
PsrI	GAACNNNNNNTAC	4 cut(s)	1275, 1307, 1308, 1340
PstI	CTGCAG	4 cut(s)	514, 811, 1120, 1560
PsuI	RGATCY	4 cut(s)	44, 142, 976, 1003
PsyI	GACNNNGTC	1 cut(s)	375
PvuII	CAGCTG	2 cut(s)	632, 695
RsaI	GTAC	6 cut(s)	5, 657, 709, 942, 1293, 1407
RsaNI	GTAC	6 cut(s)	4, 656, 708, 941, 1292, 1406
RseI	CAYNNNNRTG	2 cut(s)	584, 719
Rsr2I	CGGWCCG	1 cut(s)	9
RsrII	CGGWCCG	1 cut(s)	9
SaqAI	TTAA	6 cut(s)	179, 249, 995, 1167, 1305, 1322
Sau3AI	GATC	8 cut(s)	44, 82, 142, 172, 976, 1003, 1009, 1276
Sau96I	GGNCC	5 cut(s)	9, 371, 479, 1100, 1654
ScrFI	CCNGG	4 cut(s)	37, 351, 375, 902
SduI	GDGCHC	1 cut(s)	1255
SexAI	ACCWGGT	1 cut(s)	373
SfaNI	GCATC	4 cut(s)	121, 289, 404, 1373
SfcI	CTRYAG	5 cut(s)	18, 510, 807, 1116, 1556
SinI	GGWCC	5 cut(s)	9, 371, 479, 1100, 1654
SmiMI	CAYNNNNRTG	2 cut(s)	584, 719
SmlI	CTYRAG	2 cut(s)	223, 1044
SmoI	CTYRAG	2 cut(s)	223, 1044
SphI	GCATGC	2 cut(s)	362, 1448
SsiI	CCGC	5 cut(s)	42, 302, 365, 383, 752
SspI	AATATT	2 cut(s)	886, 1491
SspMI	CTAG	2 cut(s)	456, 1280
StyD4I	CCNGG	4 cut(s)	35, 349, 373, 900
StyI	CCWWGG	3 cut(s)	123, 281, 1093
TaaI	ACNGT	7 cut(s)	13, 96, 475, 655, 1506, 1634, 1686
TaiI	ACGT	3 cut(s)	664, 1438, 1458
TaqI	TCGA	4 cut(s)	291, 797, 1567, 1681
TaqII	GACCGA	1 cut(s)	467
TatI	WGTACW	2 cut(s)	707, 1291
TauI	GCSGC	1 cut(s)	368
TfiI	GAWTC	2 cut(s)	286, 965
Tru1I	TTAA	6 cut(s)	179, 249, 995, 1167, 1305, 1322
Tru9I	TTAA	6 cut(s)	179, 249, 995, 1167, 1305, 1322
TscAI	CASTG	2 cut(s)	405, 498
TseFI	GTSAC	1 cut(s)	73
TseI	GCWGC	9 cut(s)	362, 509, 571, 632, 692, 1115, 1361, 1484, 1558
Tsp45I	GTSAC	1 cut(s)	73
TspDTI	ATGAA	9 cut(s)	153, 258, 348, 837, 957, 976, 1124, 1205, 1412
TspRI	CASTG	2 cut(s)	405, 498
Tth111I	GACNNNGTC	1 cut(s)	375
VpaK11BI	GGWCC	5 cut(s)	9, 371, 479, 1100, 1654
VspI	ATTAAT	1 cut(s)	249
XapI	RAATTY	4 cut(s)	157, 310, 1074, 1159
XbaI	TCTAGA	1 cut(s)	1279
XceI	RCATGY	2 cut(s)	362, 1448
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1389
XmiI	GTMKAC	1 cut(s)	1347
XspI	CTAG	2 cut(s)	456, 1280

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.