AT1G72930

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0016593
Physical Location & Seq
Forward (+)
27439240 .. 27440464
1225 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G72930.1

Sequence Viewer

Length: 531 bp
ATGTCTTCTCATACTGCAACTAAGTATGATGTTTTCCTAAGTTTCAGAGGGCATGACACTCGCCACAACTTCATCAGTTTTCTCTACAAAGAACTGGTTCGAAGGAGCATTCGAACCTTCAAAGACGACAAAGAGCTGGAGAATGGCCAGAGGTTTTCGCCGGAGCTCAAAAGCCCCATCGAGGTGTCAAGATTCGCCGTCGTTGTTGTCTCAGAGAACTATGCTGCGTCTTCTTGGTGTCTCGATGAGCTCGTAACGATCATGGATTTCGAAAAAAAGGGTTCCATCACCGTGATGCCCATCTTCTACGGCGTGGAACCGAATCATGTGAGGTGGCAGACCGGAGTACTCGCTGAACAGTTTAAGAAACATGCGAGTAGAGAAGATCCTGAGAAAGTTCTTAAATGGAGGCAAGCATTGACCAATTTTGCGCAACTCTCCGGCGATTGTTCAGGTGATGATGACTCGAAGCTGGTGGACAAAATTGCTAACGAGATATCAAACAAGAAGACGATTTATGCAACAATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

176

Amino Acids

20.24

Weight (kDa)

6.97

Isoelectric Point (pI)

43.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 8 - 172 1.7e-62 TIR domain
TIR_2 PF13676 11 - 107 2.4e-14 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 432
AclWI GGATC 1 cut(s) 380
AcoI YGGCCR 1 cut(s) 145
AfaI GTAC 1 cut(s) 348
AfiI CCNNNNNNNGG 1 cut(s) 181
AgsI TTSAA 1 cut(s) 121
AluBI AGCT 4 cut(s) 136, 166, 250, 472
AluI AGCT 4 cut(s) 136, 166, 250, 472
Alw21I GWGCWC 2 cut(s) 168, 252
Alw26I GTCTC 2 cut(s) 214, 245
AlwI GGATC 1 cut(s) 380
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 224
Asp700I GAANNNNTTC 1 cut(s) 96
AspLEI GCGC 1 cut(s) 433
AsuHPI GGTGA 2 cut(s) 280, 467
AsuII TTCGAA 3 cut(s) 100, 112, 270
BalI TGGCCA 1 cut(s) 147
BanII GRGCYC 2 cut(s) 168, 252
BbsI GAAGAC 2 cut(s) 222, 515
Bbv12I GWGCWC 2 cut(s) 168, 252
BbvI GCAGC 1 cut(s) 211
BccI CCATC 3 cut(s) 185, 293, 308
BceAI ACGGC 2 cut(s) 182, 325
BcgI CGANNNNNNTGC 2 cut(s) 41, 75
BcoDI GTCTC 2 cut(s) 214, 245
BisI GCNGC 1 cut(s) 225
BlsI GCNGC 1 cut(s) 226
BmcAI AGTACT 1 cut(s) 348
BmiI GGNNCC 2 cut(s) 283, 318
BmsI GCATC 1 cut(s) 285
BpiI GAAGAC 2 cut(s) 222, 515
BpmI CTGGAG 1 cut(s) 158
Bpu14I TTCGAA 3 cut(s) 100, 112, 270
BsaBI GATNNNNATC 1 cut(s) 299
BsaWI WCCGGW 1 cut(s) 341
Bsc4I CCNNNNNNNGG 1 cut(s) 181
Bse1I ACTGG 1 cut(s) 99
Bse8I GATNNNNATC 1 cut(s) 299
BseJI GATNNNNATC 1 cut(s) 299
BseLI CCNNNNNNNGG 1 cut(s) 181
BseMII CTCAG 2 cut(s) 225, 381
BseNI ACTGG 1 cut(s) 99
BseXI GCAGC 1 cut(s) 211
BshFI GGCC 1 cut(s) 147
BsiHKAI GWGCWC 2 cut(s) 168, 252
BsiSI CCGG 3 cut(s) 161, 342, 441
BslI CCNNNNNNNGG 1 cut(s) 181
BsmAI GTCTC 2 cut(s) 214, 245
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 1 cut(s) 147
Bsp119I TTCGAA 3 cut(s) 100, 112, 270
Bsp1286I GDGCHC 2 cut(s) 168, 252
Bsp143I GATC 2 cut(s) 258, 385
BspANI GGCC 1 cut(s) 147
BspCNI CTCAG 2 cut(s) 224, 382
BspLI GGNNCC 2 cut(s) 283, 318
BspPI GGATC 1 cut(s) 380
BspT104I TTCGAA 3 cut(s) 100, 112, 270
BsrI ACTGG 1 cut(s) 99
BssMI GATC 2 cut(s) 258, 385
Bst4CI ACNGT 2 cut(s) 292, 360
BstBI TTCGAA 3 cut(s) 100, 112, 270
BstC8I GCNNGC 1 cut(s) 414
BstDEI CTNAG 4 cut(s) 21, 38, 211, 390
BstHHI GCGC 1 cut(s) 433
BstKTI GATC 2 cut(s) 261, 388
BstMAI GTCTC 2 cut(s) 214, 245
BstMBI GATC 2 cut(s) 258, 385
BstNSI RCATGY 1 cut(s) 374
BstV1I GCAGC 1 cut(s) 211
BstV2I GAAGAC 2 cut(s) 222, 515
BstX2I RGATCY 1 cut(s) 385
BstYI RGATCY 1 cut(s) 385
BsuRI GGCC 1 cut(s) 147
Cac8I GCNNGC 1 cut(s) 414
CfoI GCGC 1 cut(s) 433
CseI GACGC 1 cut(s) 216
Csp6I GTAC 1 cut(s) 347
CviAII CATG 4 cut(s) 53, 262, 326, 371
CviJI RGCY 6 cut(s) 136, 147, 166, 174, 250, 472
CviKI_1 RGCY 6 cut(s) 136, 147, 166, 174, 250, 472
CviQI GTAC 1 cut(s) 347
DdeI CTNAG 4 cut(s) 21, 38, 211, 390
DpnI GATC 2 cut(s) 260, 387
DpnII GATC 2 cut(s) 258, 385
EaeI YGGCCR 1 cut(s) 145
Ecl136II GAGCTC 2 cut(s) 166, 250
Eco24I GRGCYC 2 cut(s) 168, 252
Eco32I GATATC 1 cut(s) 498
Eco53kI GAGCTC 2 cut(s) 166, 250
EcoICRI GAGCTC 2 cut(s) 166, 250
EcoRV GATATC 1 cut(s) 498
EcoT38I GRGCYC 2 cut(s) 168, 252
FaeI CATG 4 cut(s) 56, 265, 329, 374
FaiI YATR 9 cut(s) 12, 27, 54, 222, 263, 327, 372, 519, 529
FatI CATG 4 cut(s) 52, 261, 325, 370
Fnu4HI GCNGC 1 cut(s) 225
FriOI GRGCYC 2 cut(s) 168, 252
Fsp4HI GCNGC 1 cut(s) 225
FspI TGCGCA 1 cut(s) 432
GlaI GCGC 1 cut(s) 432
GluI GCNGC 1 cut(s) 225
GsuI CTGGAG 1 cut(s) 158
HaeIII GGCC 1 cut(s) 147
HapII CCGG 3 cut(s) 161, 342, 441
HgaI GACGC 1 cut(s) 216
HhaI GCGC 1 cut(s) 433
Hin1II CATG 4 cut(s) 56, 265, 329, 374
Hin6I GCGC 1 cut(s) 431
HinP1I GCGC 1 cut(s) 431
HinfI GANTC 3 cut(s) 192, 322, 464
HpaII CCGG 3 cut(s) 161, 342, 441
HphI GGTGA 2 cut(s) 280, 467
Hpy166II GTNNAC 1 cut(s) 478
Hpy188I TCNGA 2 cut(s) 47, 214
Hpy188III TCNNGA 3 cut(s) 189, 242, 389
Hpy8I GTNNAC 1 cut(s) 478
Hpy99I CGWCG 1 cut(s) 203
HpyAV CCTTC 2 cut(s) 96, 127
HpyCH4III ACNGT 2 cut(s) 292, 360
HpyCH4V TGCA 2 cut(s) 17, 521
HpyF3I CTNAG 4 cut(s) 21, 38, 211, 390
Hsp92II CATG 4 cut(s) 56, 265, 329, 374
HspAI GCGC 1 cut(s) 431
Kzo9I GATC 2 cut(s) 258, 385
LmnI GCTCC 2 cut(s) 105, 163
LpnPI CCDG 9 cut(s) 80, 122, 161, 174, 355, 402, 438, 454, 458
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 285
MaeIII GTNAC 1 cut(s) 253
MalI GATC 2 cut(s) 260, 387
MboI GATC 2 cut(s) 258, 385
MboII GAAGA 4 cut(s) 222, 295, 395, 520
MflI RGATCY 1 cut(s) 385
MhlI GDGCHC 2 cut(s) 168, 252
MlsI TGGCCA 1 cut(s) 147
MluCI AATT 2 cut(s) 424, 483
MluNI TGGCCA 1 cut(s) 147
MlyI GAGTC 1 cut(s) 458
MnlI CCTC 5 cut(s) 41, 144, 175, 324, 402
Mox20I TGGCCA 1 cut(s) 147
MroXI GAANNNNTTC 1 cut(s) 96
MscI TGGCCA 1 cut(s) 147
MseI TTAA 2 cut(s) 363, 402
MslI CAYNNNNRTG 3 cut(s) 182, 290, 293
Msp20I TGGCCA 1 cut(s) 147
MspI CCGG 3 cut(s) 161, 342, 441
Mva1269I GAATGC 1 cut(s) 108
NdeII GATC 2 cut(s) 258, 385
NlaIII CATG 4 cut(s) 56, 265, 329, 374
NlaIV GGNNCC 2 cut(s) 283, 318
NsbI TGCGCA 1 cut(s) 432
NspI RCATGY 1 cut(s) 374
NspV TTCGAA 3 cut(s) 100, 112, 270
PcsI WCGNNNNNNNCGW 1 cut(s) 249
PctI GAATGC 1 cut(s) 108
PdmI GAANNNNTTC 1 cut(s) 96
PfeI GAWTC 2 cut(s) 192, 322
PkrI GCNGC 1 cut(s) 226
PleI GAGTC 1 cut(s) 458
PpsI GAGTC 1 cut(s) 458
Psp124BI GAGCTC 2 cut(s) 168, 252
PspN4I GGNNCC 2 cut(s) 283, 318
PsuI RGATCY 1 cut(s) 385
RsaI GTAC 1 cut(s) 348
RsaNI GTAC 1 cut(s) 347
RseI CAYNNNNRTG 3 cut(s) 182, 290, 293
SacI GAGCTC 2 cut(s) 168, 252
SaqAI TTAA 2 cut(s) 363, 402
SatI GCNGC 1 cut(s) 225
Sau3AI GATC 2 cut(s) 258, 385
ScaI AGTACT 1 cut(s) 348
SchI GAGTC 1 cut(s) 458
SduI GDGCHC 2 cut(s) 168, 252
SetI ASST 9 cut(s) 119, 138, 155, 168, 186, 252, 335, 457, 474
SfaNI GCATC 1 cut(s) 285
SfuI TTCGAA 3 cut(s) 100, 112, 270
SmiMI CAYNNNNRTG 3 cut(s) 182, 290, 293
Sse9I AATT 2 cut(s) 424, 483
SstI GAGCTC 2 cut(s) 168, 252
TaaI ACNGT 2 cut(s) 292, 360
TaqI TCGA 6 cut(s) 100, 112, 180, 243, 270, 467
TasI AATT 2 cut(s) 424, 483
TatI WGTACW 1 cut(s) 346
TfiI GAWTC 2 cut(s) 192, 322
Tru1I TTAA 2 cut(s) 363, 402
Tru9I TTAA 2 cut(s) 363, 402
TseI GCWGC 1 cut(s) 224
TspDTI ATGAA 1 cut(s) 61
XceI RCATGY 1 cut(s) 374
XmnI GAANNNNTTC 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.