MD12G1010100.v1.1

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0016593
Physical Location & Seq
Forward (+)
1033636 .. 1036384
2749 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1010100.v1.1.491

Sequence Viewer

Length: 1251 bp
ATGGAGAACGAAAACGAAGGGTGGATATACGACGTGTTCGTCAACTTCCGCGGCAAAGACACCGGCCGTAACTTCACCGACCACCTCTGCGCCGCCCTGGACCAGAAGGGGATCATGGCCTTCCGGTACGATCAGGCACTCGAGAGAGGAAAGCCCATTTCCTCCGAGATCCTCAAGTCGATCGGAGAATCGAGGTTTTCGATCGTAGTCTTCTCGAAAAACTACGCCGCCTCGGCGCGGCGCTTGGACGAGCTCGTGGAGATCCTCCGGTGCATGAAATCGACGGGCCAGATCGTTTTTCCGGTGTTCTACGATGTGGATCCGTCGGATGTGCGGAAGCAGAGGGGGGAGTATTTTGAGAAGGCGTTCAGGAAGCATGAGGAGGATTTCATGGACAAGGTGGAGAGTTGGAGAGGTGCATTGAGAGATGCAGCCACCCTCTCTGGGTGGAATTTGTATAACAAGCACGAGGCAAAAGTTATCCAAGAGATTGTCAAAGAGATATCAAATCAATTGAGCCAAAAGCTTTCAAGCGTGCCCGAGGATTTATTTGGAATCGATTCCCGAGTGGAGGAAATGAATCTCTGCTTGGATATGGAGGTGAACGATGTTCGCATTATAGGAATTTACGGGGAGAAAGGTATAGGCAAGACGACAATTGCTCAAGTTGTTTACGACAGGGTATCTGGTCAGTTCAACGGCAGCAGCTTCCTTGCTAACATAAAAGAAGTTGATGAAAAACAAGGCCTAGTTCCTTTACAGGAACAACTTATTTCGGAAATCCTCATGGATAAGAATATTAAGATATGGAATGTACATATAGGAGTCAATGCAATCCTGAACAGACTGCGCTATAAAAAGGTTCTTCTCGTTCTTGATGGCGTGGATCGGTTAGAACAGTTAGAAGCCTTGGCTGGGAGCCGCGATTGGTTTGGCGCAGGAAGTAGAATCATTGTAACAACCAGGGATGAAAACTTGCTGATCAGATACCGTGTTGACAGGTTTTATAGGGCACAGGCGTTGAATCACGAAGAAGCGCTTCGCCTTTTTTGTTGGAAAGCGTTTAAACAAGGCCATCCGCCGGAGGAGCAGTATGTAGAGCTGTGCGAGCTTGTGTTGAATCATGCTCGCGGCCTTCCGTCTGCGCTTGAAGCTTTGGGGTCTTTCTTGTTTGGCAGGAGTTTAGTTGAATGGAGAAGTGCAGTAAATAGACTAGCACAAGTTCCTTGCACGTGGAATTACTTGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

417

Amino Acids

47.8

Weight (kDa)

6.08

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 10 - 178 9.5e-50 TIR domain
TIR_2 PF13676 12 - 109 2.4e-11 TIR domain
NB-ARC PF00931 199 - 357 2.5e-19 NB-ARC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 38
AccII CGCG 4 cut(s) 51, 238, 924, 1131
AciI CCGC 9 cut(s) 49, 51, 93, 228, 238, 334, 922, 1079, 1131
AclWI GGATC 6 cut(s) 119, 163, 256, 314, 327, 894
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 451, 624
AcvI CACGTG 1 cut(s) 1233
AfaI GTAC 2 cut(s) 128, 816
AfeI AGCGCT 1 cut(s) 1038
AfiI CCNNNNNNNGG 5 cut(s) 237, 444, 571, 915, 1081
AflIII ACRYGT 1 cut(s) 33
AgsI TTSAA 7 cut(s) 531, 697, 1024, 1120, 1151, 1190, 1246
AjiI CACGTC 1 cut(s) 34
AjnI CCWGG 2 cut(s) 96, 962
AjuI GAANNNNNNNTTGG 2 cut(s) 572, 604
AluBI AGCT 6 cut(s) 253, 526, 708, 1102, 1111, 1154
AluI AGCT 6 cut(s) 253, 526, 708, 1102, 1111, 1154
Alw21I GWGCWC 1 cut(s) 255
AlwI GGATC 6 cut(s) 119, 163, 256, 314, 327, 894
Ama87I CYCGRG 3 cut(s) 140, 539, 564
Aor51HI AGCGCT 1 cut(s) 1038
AoxI GGCC 6 cut(s) 64, 117, 286, 745, 1072, 1132
ApeKI GCWGC 3 cut(s) 431, 702, 705
ApoI RAATTY 2 cut(s) 451, 624
Asp700I GAANNNNTTC 3 cut(s) 365, 559, 1038
AspLEI GCGC 7 cut(s) 92, 238, 243, 852, 938, 1039, 1147
AspS9I GGNCC 2 cut(s) 100, 286
AsuHPI GGTGA 2 cut(s) 67, 613
AvaI CYCGRG 3 cut(s) 140, 539, 564
AvaII GGWCC 1 cut(s) 100
BaeGI GKGCMC 2 cut(s) 540, 1015
BamHI GGATCC 1 cut(s) 319
BanII GRGCYC 1 cut(s) 255
BauI CACGAG 2 cut(s) 254, 467
BbrPI CACGTG 1 cut(s) 1233
BbsI GAAGAC 1 cut(s) 202
Bbv12I GWGCWC 1 cut(s) 255
BbvI GCAGC 3 cut(s) 443, 714, 717
BccI CCATC 2 cut(s) 872, 1083
BceAI ACGGC 2 cut(s) 51, 715
BciT130I CCWGG 2 cut(s) 98, 964
BclI TGATCA 1 cut(s) 981
BfaI CTAG 2 cut(s) 749, 1214
BfoI RGCGCY 2 cut(s) 244, 1040
BglI GCCNNNNNGGC 1 cut(s) 233
BisI GCNGC 9 cut(s) 52, 93, 228, 239, 432, 703, 706, 922, 1132
BlsI GCNGC 9 cut(s) 53, 94, 229, 240, 433, 704, 707, 923, 1133
Bme1390I CCNGG 2 cut(s) 98, 964
Bme18I GGWCC 1 cut(s) 100
BmeT110I CYCGRG 3 cut(s) 140, 539, 564
BmgBI CACGTC 1 cut(s) 34
BmgT120I GGNCC 2 cut(s) 100, 286
BmiI GGNNCC 2 cut(s) 321, 920
BmrFI CCNGG 2 cut(s) 98, 964
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 1 cut(s) 202
BpuEI CTTGAG 2 cut(s) 158, 648
Bsa29I ATCGAT 1 cut(s) 558
BsaAI YACGTR 1 cut(s) 1233
BsaBI GATNNNNATC 1 cut(s) 318
BsaJI CCNNGG 6 cut(s) 49, 96, 231, 540, 909, 963
BsaWI WCCGGW 3 cut(s) 123, 267, 301
BsaXI ACNNNNNCTCC 2 cut(s) 1076, 1106
Bsc4I CCNNNNNNNGG 5 cut(s) 237, 444, 571, 915, 1081
Bse118I RCCGGY 1 cut(s) 62
Bse8I GATNNNNATC 1 cut(s) 318
BseBI CCWGG 2 cut(s) 98, 964
BseCI ATCGAT 1 cut(s) 558
BseDI CCNNGG 6 cut(s) 49, 96, 231, 540, 909, 963
BseGI GGATG 3 cut(s) 334, 973, 1075
BseJI GATNNNNATC 1 cut(s) 318
BseLI CCNNNNNNNGG 5 cut(s) 237, 444, 571, 915, 1081
BseRI GAGGAG 2 cut(s) 395, 1100
BseSI GKGCMC 2 cut(s) 540, 1015
BseX3I CGGCCG 1 cut(s) 64
BseXI GCAGC 3 cut(s) 443, 714, 717
BseYI CCCAGC 1 cut(s) 914
BsgI GTGCAG 1 cut(s) 1221
Bsh1236I CGCG 4 cut(s) 51, 238, 924, 1131
Bsh1285I CGRYCG 3 cut(s) 67, 183, 204
BshFI GGCC 6 cut(s) 66, 119, 288, 747, 1074, 1134
BshVI ATCGAT 1 cut(s) 558
BsiEI CGRYCG 3 cut(s) 67, 183, 204
BsiHKAI GWGCWC 1 cut(s) 255
BsiHKCI CYCGRG 3 cut(s) 140, 539, 564
BsiSI CCGG 5 cut(s) 63, 124, 268, 302, 1082
BslI CCNNNNNNNGG 5 cut(s) 237, 444, 571, 915, 1081
BsnI GGCC 6 cut(s) 66, 119, 288, 747, 1074, 1134
BsoBI CYCGRG 3 cut(s) 140, 539, 564
Bsp1286I GDGCHC 3 cut(s) 255, 540, 1015
Bsp1407I TGTACA 1 cut(s) 814
BspACI CCGC 9 cut(s) 49, 51, 93, 228, 238, 334, 922, 1079, 1131
BspANI GGCC 6 cut(s) 66, 119, 288, 747, 1074, 1134
BspDI ATCGAT 1 cut(s) 558
BspFNI CGCG 4 cut(s) 51, 238, 924, 1131
BspLI GGNNCC 2 cut(s) 321, 920
BspPI GGATC 6 cut(s) 119, 163, 256, 314, 327, 894
BsrFI RCCGGY 1 cut(s) 62
BsrGI TGTACA 1 cut(s) 814
BssAI RCCGGY 1 cut(s) 62
BssECI CCNNGG 6 cut(s) 49, 96, 231, 540, 909, 963
BssSI CACGAG 2 cut(s) 254, 467
BssT1I CCWWGG 1 cut(s) 909
Bst2BI CACGAG 2 cut(s) 254, 467
Bst2UI CCWGG 2 cut(s) 98, 964
Bst4CI ACNGT 2 cut(s) 900, 992
BstAUI TGTACA 1 cut(s) 814
BstBAI YACGTR 1 cut(s) 1233
BstC8I GCNNGC 3 cut(s) 536, 1109, 1129
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 3 cut(s) 334, 973, 1075
BstFNI CGCG 4 cut(s) 51, 238, 924, 1131
BstH2I RGCGCY 2 cut(s) 244, 1040
BstHHI GCGC 7 cut(s) 92, 238, 243, 852, 938, 1039, 1147
BstMCI CGRYCG 3 cut(s) 67, 183, 204
BstMWI GCNNNNNNNGC 4 cut(s) 233, 1087, 1108, 1151
BstNI CCWGG 2 cut(s) 98, 964
BstSCI CCNGG 2 cut(s) 96, 962
BstSLI GKGCMC 2 cut(s) 540, 1015
BstUI CGCG 4 cut(s) 51, 238, 924, 1131
BstV1I GCAGC 3 cut(s) 443, 714, 717
BstV2I GAAGAC 1 cut(s) 202
BstX2I RGATCY 3 cut(s) 168, 261, 319
BstYI RGATCY 3 cut(s) 168, 261, 319
BstZI CGGCCG 1 cut(s) 64
Bsu15I ATCGAT 1 cut(s) 558
BsuRI GGCC 6 cut(s) 66, 119, 288, 747, 1074, 1134
BsuTUI ATCGAT 1 cut(s) 558
BtgI CCRYGG 1 cut(s) 49
BtrI CACGTC 1 cut(s) 34
BtsCI GGATG 3 cut(s) 334, 973, 1075
Cac8I GCNNGC 3 cut(s) 536, 1109, 1129
CfoI GCGC 7 cut(s) 92, 238, 243, 852, 938, 1039, 1147
Cfr10I RCCGGY 1 cut(s) 62
Cfr13I GGNCC 2 cut(s) 100, 286
Cfr42I CCGCGG 1 cut(s) 52
ClaI ATCGAT 1 cut(s) 558
Csp6I GTAC 2 cut(s) 127, 815
CviAII CATG 6 cut(s) 115, 274, 377, 391, 787, 1124
CviQI GTAC 2 cut(s) 127, 815
DraI TTTAAA 1 cut(s) 1066
DrdI GACNNNNNNGTC 1 cut(s) 38
DseDI GACNNNNNNGTC 1 cut(s) 38
EaeI YGGCCR 1 cut(s) 64
EagI CGGCCG 1 cut(s) 64
EciI GGCGGA 1 cut(s) 1068
Ecl136II GAGCTC 1 cut(s) 253
EclXI CGGCCG 1 cut(s) 64
Eco130I CCWWGG 1 cut(s) 909
Eco147I AGGCCT 1 cut(s) 747
Eco24I GRGCYC 1 cut(s) 255
Eco32I GATATC 1 cut(s) 504
Eco47I GGWCC 1 cut(s) 100
Eco47III AGCGCT 1 cut(s) 1038
Eco52I CGGCCG 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 253
Eco72I CACGTG 1 cut(s) 1233
Eco88I CYCGRG 3 cut(s) 140, 539, 564
EcoICRI GAGCTC 1 cut(s) 253
EcoRII CCWGG 2 cut(s) 96, 962
EcoRV GATATC 1 cut(s) 504
EcoT14I CCWWGG 1 cut(s) 909
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 909
FaeI CATG 6 cut(s) 118, 277, 380, 394, 790, 1127
FalI AAGNNNNNCTT 2 cut(s) 1023, 1055
FatI CATG 6 cut(s) 114, 273, 376, 390, 786, 1123
FbaI TGATCA 1 cut(s) 981
Fnu4HI GCNGC 9 cut(s) 52, 93, 228, 239, 432, 703, 706, 922, 1132
FokI GGATG 3 cut(s) 341, 980, 1062
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 9 cut(s) 52, 93, 228, 239, 432, 703, 706, 922, 1132
FspBI CTAG 2 cut(s) 749, 1214
GlaI GCGC 7 cut(s) 91, 237, 242, 851, 937, 1038, 1146
GluI GCNGC 9 cut(s) 52, 93, 228, 239, 432, 703, 706, 922, 1132
GsaI CCCAGC 1 cut(s) 918
HaeII RGCGCY 2 cut(s) 244, 1040
HaeIII GGCC 6 cut(s) 66, 119, 288, 747, 1074, 1134
HapII CCGG 5 cut(s) 63, 124, 268, 302, 1082
HhaI GCGC 7 cut(s) 92, 238, 243, 852, 938, 1039, 1147
Hin1II CATG 6 cut(s) 118, 277, 380, 394, 790, 1127
Hin6I GCGC 7 cut(s) 90, 236, 241, 850, 936, 1037, 1145
HinP1I GCGC 7 cut(s) 90, 236, 241, 850, 936, 1037, 1145
HincII GTYRAC 2 cut(s) 43, 997
HindII GTYRAC 2 cut(s) 43, 997
HindIII AAGCTT 2 cut(s) 524, 1152
HinfI GANTC 8 cut(s) 188, 555, 560, 580, 825, 948, 1024, 1120
HpaII CCGG 5 cut(s) 63, 124, 268, 302, 1082
HphI GGTGA 2 cut(s) 67, 613
Hpy166II GTNNAC 4 cut(s) 43, 604, 673, 997
Hpy188I TCNGA 5 cut(s) 166, 185, 328, 778, 986
Hpy188III TCNNGA 7 cut(s) 142, 214, 370, 564, 838, 875, 1028
Hpy8I GTNNAC 4 cut(s) 43, 604, 673, 997
Hpy99I CGWCG 3 cut(s) 35, 286, 328
HpyAV CCTTC 5 cut(s) 11, 100, 130, 355, 1145
HpyCH4III ACNGT 2 cut(s) 900, 992
HpyCH4IV ACGT 2 cut(s) 33, 1232
HpyCH4V TGCA 6 cut(s) 273, 419, 431, 833, 1202, 1230
HpyF10VI GCNNNNNNNGC 4 cut(s) 233, 1087, 1108, 1151
HpySE526I ACGT 2 cut(s) 33, 1232
Hsp92II CATG 6 cut(s) 118, 277, 380, 394, 790, 1127
HspAI GCGC 7 cut(s) 90, 236, 241, 850, 936, 1037, 1145
Ksp22I TGATCA 1 cut(s) 981
KspI CCGCGG 1 cut(s) 52
LmnI GCTCC 2 cut(s) 918, 1087
Lsp1109I GCAGC 3 cut(s) 443, 714, 717
LweI GCATC 1 cut(s) 418
MaeI CTAG 2 cut(s) 749, 1214
MaeII ACGT 2 cut(s) 33, 1232
MaeIII GTNAC 2 cut(s) 68, 955
MboII GAAGA 3 cut(s) 202, 857, 1043
MfeI CAATTG 2 cut(s) 512, 657
MflI RGATCY 3 cut(s) 168, 261, 319
MhlI GDGCHC 3 cut(s) 255, 540, 1015
MluCI AATT 5 cut(s) 451, 512, 624, 657, 1237
MlyI GAGTC 1 cut(s) 834
MmeI TCCRAC 3 cut(s) 306, 389, 1034
MroXI GAANNNNTTC 3 cut(s) 365, 559, 1038
MseI TTAA 2 cut(s) 801, 1065
MspA1I CMGCKG 1 cut(s) 51
MspI CCGG 5 cut(s) 63, 124, 268, 302, 1082
MspR9I CCNGG 2 cut(s) 98, 964
MssI GTTTAAAC 1 cut(s) 1066
MteI GCGCNGCGC 1 cut(s) 239
MunI CAATTG 2 cut(s) 512, 657
MvaI CCWGG 2 cut(s) 98, 964
MvnI CGCG 4 cut(s) 51, 238, 924, 1131
MwoI GCNNNNNNNGC 4 cut(s) 233, 1087, 1108, 1151
NlaIII CATG 6 cut(s) 118, 277, 380, 394, 790, 1127
NlaIV GGNNCC 2 cut(s) 321, 920
NmeAIII GCCGAG 1 cut(s) 212
PaeR7I CTCGAG 1 cut(s) 140
PceI AGGCCT 1 cut(s) 747
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 197
PdmI GAANNNNTTC 3 cut(s) 365, 559, 1038
PfeI GAWTC 7 cut(s) 188, 555, 560, 580, 948, 1024, 1120
PkrI GCNGC 9 cut(s) 53, 94, 229, 240, 433, 704, 707, 923, 1133
Ple19I CGATCG 2 cut(s) 183, 204
PleI GAGTC 1 cut(s) 833
PmaCI CACGTG 1 cut(s) 1233
PmeI GTTTAAAC 1 cut(s) 1066
PmlI CACGTG 1 cut(s) 1233
PpsI GAGTC 1 cut(s) 833
Ppu21I YACGTR 1 cut(s) 1233
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 96, 962
PspCI CACGTG 1 cut(s) 1233
PspFI CCCAGC 1 cut(s) 914
PspGI CCWGG 2 cut(s) 96, 962
PspN4I GGNNCC 2 cut(s) 321, 920
PspPI GGNCC 2 cut(s) 100, 286
PsuI RGATCY 3 cut(s) 168, 261, 319
PvuI CGATCG 2 cut(s) 183, 204
RsaI GTAC 2 cut(s) 128, 816
RsaNI GTAC 2 cut(s) 127, 815
SacI GAGCTC 1 cut(s) 255
SacII CCGCGG 1 cut(s) 52
SaqAI TTAA 2 cut(s) 801, 1065
SatI GCNGC 9 cut(s) 52, 93, 228, 239, 432, 703, 706, 922, 1132
Sau96I GGNCC 2 cut(s) 100, 286
SchI GAGTC 1 cut(s) 834
ScrFI CCNGG 2 cut(s) 98, 964
SduI GDGCHC 3 cut(s) 255, 540, 1015
SfaNI GCATC 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 140
Sfr303I CCGCGG 1 cut(s) 52
SgrBI CCGCGG 1 cut(s) 52
SinI GGWCC 1 cut(s) 100
SlaI CTCGAG 1 cut(s) 140
SmlI CTYRAG 3 cut(s) 140, 173, 663
SmoI CTYRAG 3 cut(s) 140, 173, 663
Sse9I AATT 5 cut(s) 451, 512, 624, 657, 1237
SseBI AGGCCT 1 cut(s) 747
SsiI CCGC 9 cut(s) 49, 51, 93, 228, 238, 334, 922, 1079, 1131
SspI AATATT 1 cut(s) 799
SspMI CTAG 2 cut(s) 749, 1214
SstI GAGCTC 1 cut(s) 255
StuI AGGCCT 1 cut(s) 747
StyD4I CCNGG 2 cut(s) 96, 962
StyI CCWWGG 1 cut(s) 909
TaaI ACNGT 2 cut(s) 900, 992
TaiI ACGT 2 cut(s) 36, 1235
TaqI TCGA 7 cut(s) 141, 179, 191, 200, 215, 281, 558
TasI AATT 5 cut(s) 451, 512, 624, 657, 1237
TatI WGTACW 1 cut(s) 814
TauI GCSGC 6 cut(s) 54, 95, 230, 241, 924, 1134
TfiI GAWTC 7 cut(s) 188, 555, 560, 580, 948, 1024, 1120
Tru1I TTAA 2 cut(s) 801, 1065
Tru9I TTAA 2 cut(s) 801, 1065
TseI GCWGC 3 cut(s) 431, 702, 705
TspDTI ATGAA 5 cut(s) 290, 379, 593, 750, 984
TspGWI ACGGA 2 cut(s) 312, 1128
VpaK11BI GGWCC 1 cut(s) 100
XapI RAATTY 2 cut(s) 451, 624
XhoI CTCGAG 1 cut(s) 140
XmnI GAANNNNTTC 3 cut(s) 365, 559, 1038
XspI CTAG 2 cut(s) 749, 1214
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.