AT1G75540

sth2 sth2 (salt tolerance homolog2)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0011847
Physical Location & Seq
Forward (+)
28365824 .. 28367738
1915 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75540.1

Sequence Viewer

Length: 996 bp
ATGAAGATCAGGTGCGACGTCTGCGATAAAGAAGAAGCGTCGGTGTTTTGCACGGCCGACGAAGCATCTCTCTGCGGCGGCTGCGACCACCAAGTCCACCACGCTAACAAACTCGCCTCTAAACATCTCCGTTTCTCTCTCCTTTATCCTTCTTCTTCCAACACCTCCTCTCCTCTCTGCGACATCTGTCAGGATAAAAAAGCTCTGTTGTTCTGTCAACAAGATAGAGCTATTTTATGCAAAGATTGCGATTCATCGATCCACGCTGCGAACGAACACACAAAGAAACACGATAGGTTTCTTCTTACAGGGGTTAAGCTCTCTGCAACATCGTCTGTTTACAAACCTACTTCGAAATCTTCTTCTTCTTCTTCAAGCAACCAAGATTTCTCTGTCCCTGGATCATCAATCTCTAATCCTCCTCCTCTCAAGAAACCTCTCTCAGCTCCTCCTCAGAGCAACAAGATCCAACCCTTTTCGAAGATCAACGGCGGTGATGCGTCGGTGAATCAGTGGGGATCCACAAGCACGATTTCTGAGTATTTGATGGATACGTTACCTGGTTGGCACGTTGAGGATTTCCTCGATTCCTCTCTTCCTACTTATGGTTTCTCTAAGAGTGGTGATGATGATGGAGTGTTACCATATATGGAACCAGAAGATGACAACAACACTAAGAGAAACAACAACAACAACAACAACAACAACAACAATACAGTGTCACTTCCATCTAAGAATTTAGGGATTTGGGTCCCTCAGATTCCACAAACTCTTCCTTCTTCATACCCAAATCAATACTTTTCTCAAGACAACAACATACAGTTTGGGATGTACAACAAAGAAACATCACCAGAAGTAGTGTCTTTTGCTCCAATACAAAACATGAAACAACAAGGACAGAACAACAAGAGATGGTATGATGATGGTGGCTTCACTGTCCCACAGATCACTCCTCCTCCTCTTTCTTCTAATAAAAAGTTTAGATCTTTCTGGTAA

Protein Analysis

331

Amino Acids

36.63

Weight (kDa)

6.44

Isoelectric Point (pI)

58.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-B_box PF00643 57 - 94 5e-08 B-box zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011847)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G75540
fragaria_vesca FvH4_2g38990
malus_domestica MD08G1021000.v1.1 MD15G1018700.v1.1 MD15G1019000.v1.1
prunus_persica Prupe.1G371100_v2.0.a1 Prupe.1G371100_v2.0.a1
pyrus_communis pycom08g01660 pycom15g01580
rosa_chinensis RchiOBHm_Chr6g0302091
rosa_laevigata RLG00000011196
rosa_multiflora Rmu_ssc0000160.1_g000008
rosa_roxburghii Rroxscaffold_7G00166090
rosa_rugosa Rorug06G0312300
rosa_samantha Rh6AG424600 Rh6BG477800 Rh6CG437500 Rh6DG424500
rosa_wichuraiana Rw6G036770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AatII GACGTC 1 cut(s) 21
AciI CCGC 3 cut(s) 75, 78, 492
AclWI GGATC 5 cut(s) 253, 409, 460, 513, 526
AcoI YGGCCR 1 cut(s) 54
AcsI RAATTY 1 cut(s) 736
AcyI GRCGYC 1 cut(s) 18
AfaI GTAC 1 cut(s) 833
AfiI CCNNNNNNNGG 1 cut(s) 605
AgsI TTSAA 1 cut(s) 375
AjnI CCWGG 2 cut(s) 397, 559
AluBI AGCT 4 cut(s) 203, 230, 319, 446
AluI AGCT 4 cut(s) 203, 230, 319, 446
AlwI GGATC 5 cut(s) 253, 409, 460, 513, 526
AoxI GGCC 1 cut(s) 54
ApeKI GCWGC 2 cut(s) 81, 266
ApoI RAATTY 1 cut(s) 736
AspS9I GGNCC 1 cut(s) 751
AsuHPI GGTGA 4 cut(s) 506, 517, 635, 840
AsuII TTCGAA 2 cut(s) 353, 479
AvaII GGWCC 1 cut(s) 751
BamHI GGATCC 1 cut(s) 518
BbvI GCAGC 2 cut(s) 68, 253
BccI CCATC 5 cut(s) 541, 626, 736, 906, 917
BceAI ACGGC 2 cut(s) 69, 505
BciT130I CCWGG 2 cut(s) 399, 561
BciVI GTATCC 1 cut(s) 544
BfuI GTATCC 1 cut(s) 544
BglII AGATCT 1 cut(s) 983
BisI GCNGC 4 cut(s) 76, 79, 82, 267
BlsI GCNGC 4 cut(s) 77, 80, 83, 268
Bme1390I CCNGG 2 cut(s) 399, 561
Bme18I GGWCC 1 cut(s) 751
BmgT120I GGNCC 1 cut(s) 751
BmiI GGNNCC 4 cut(s) 520, 654, 752, 753
BmrFI CCNGG 2 cut(s) 399, 561
BmsI GCATC 2 cut(s) 74, 487
BoxI GACNNNNGTC 1 cut(s) 186
Bpu14I TTCGAA 2 cut(s) 353, 479
BpuEI CTTGAG 2 cut(s) 413, 789
Bsa29I ATCGAT 1 cut(s) 257
BsaHI GRCGYC 1 cut(s) 18
BsaJI CCNNGG 1 cut(s) 397
BsaXI ACNNNNNCTCC 4 cut(s) 154, 184, 940, 970
Bsc4I CCNNNNNNNGG 1 cut(s) 605
BseBI CCWGG 2 cut(s) 399, 561
BseCI ATCGAT 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 397
BseGI GGATG 1 cut(s) 834
BseLI CCNNNNNNNGG 1 cut(s) 605
BseMII CTCAG 4 cut(s) 456, 467, 528, 770
BseRI GAGGAG 9 cut(s) 157, 162, 411, 414, 438, 441, 942, 945, 948
BseX3I CGGCCG 1 cut(s) 54
BseXI GCAGC 2 cut(s) 68, 253
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 1 cut(s) 56
BshVI ATCGAT 1 cut(s) 257
BsiEI CGRYCG 1 cut(s) 57
BslFI GGGAC 3 cut(s) 380, 737, 923
BslI CCNNNNNNNGG 1 cut(s) 605
BsmFI GGGAC 3 cut(s) 380, 737, 923
BsnI GGCC 1 cut(s) 56
Bsp119I TTCGAA 2 cut(s) 353, 479
Bsp1407I TGTACA 1 cut(s) 831
Bsp143I GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
BspACI CCGC 3 cut(s) 75, 78, 492
BspANI GGCC 1 cut(s) 56
BspCNI CTCAG 4 cut(s) 455, 466, 529, 769
BspDI ATCGAT 1 cut(s) 257
BspLI GGNNCC 4 cut(s) 520, 654, 752, 753
BspPI GGATC 5 cut(s) 253, 409, 460, 513, 526
BspT104I TTCGAA 2 cut(s) 353, 479
BsrGI TGTACA 1 cut(s) 831
BssECI CCNNGG 1 cut(s) 397
BssMI GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
BssNI GRCGYC 1 cut(s) 18
Bst2UI CCWGG 2 cut(s) 399, 561
Bst4CI ACNGT 3 cut(s) 718, 822, 937
Bst6I CTCTTC 2 cut(s) 600, 777
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 1 cut(s) 246
BstAUI TGTACA 1 cut(s) 831
BstBI TTCGAA 2 cut(s) 353, 479
BstDEI CTNAG 7 cut(s) 442, 453, 537, 615, 675, 732, 756
BstF5I GGATG 1 cut(s) 834
BstKTI GATC 8 cut(s) 9, 261, 404, 468, 486, 521, 948, 986
BstMBI GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 4 cut(s) 21, 62, 81, 246
BstNI CCWGG 2 cut(s) 399, 561
BstPAI GACNNNNGTC 1 cut(s) 186
BstSCI CCNGG 2 cut(s) 397, 559
BstV1I GCAGC 2 cut(s) 68, 253
BstX2I RGATCY 3 cut(s) 465, 518, 983
BstYI RGATCY 3 cut(s) 465, 518, 983
BstZI CGGCCG 1 cut(s) 54
Bsu15I ATCGAT 1 cut(s) 257
BsuI GTATCC 1 cut(s) 544
BsuRI GGCC 1 cut(s) 56
BsuTUI ATCGAT 1 cut(s) 257
BtsCI GGATG 1 cut(s) 834
BtsIMutI CAGTG 3 cut(s) 518, 723, 933
Cfr13I GGNCC 1 cut(s) 751
ClaI ATCGAT 1 cut(s) 257
CseI GACGC 2 cut(s) 27, 489
CsiI ACCWGGT 1 cut(s) 559
Csp6I GTAC 1 cut(s) 832
CviAII CATG 1 cut(s) 883
CviJI RGCY 7 cut(s) 56, 81, 203, 230, 319, 446, 930
CviKI_1 RGCY 7 cut(s) 56, 81, 203, 230, 319, 446, 930
CviQI GTAC 1 cut(s) 832
DdeI CTNAG 7 cut(s) 442, 453, 537, 615, 675, 732, 756
DpnI GATC 8 cut(s) 8, 260, 403, 467, 485, 520, 947, 985
DpnII GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
EaeI YGGCCR 1 cut(s) 54
EagI CGGCCG 1 cut(s) 54
Eam1104I CTCTTC 2 cut(s) 600, 777
EarI CTCTTC 2 cut(s) 600, 777
EclXI CGGCCG 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 751
Eco52I CGGCCG 1 cut(s) 54
EcoO109I RGGNCCY 1 cut(s) 751
EcoRII CCWGG 2 cut(s) 397, 559
FaeI CATG 1 cut(s) 886
FaiI YATR 9 cut(s) 238, 606, 646, 648, 650, 784, 818, 884, 918
FaqI GGGAC 3 cut(s) 380, 737, 923
FatI CATG 1 cut(s) 882
Fnu4HI GCNGC 4 cut(s) 76, 79, 82, 267
FokI GGATG 1 cut(s) 841
Fsp4HI GCNGC 4 cut(s) 76, 79, 82, 267
GluI GCNGC 4 cut(s) 76, 79, 82, 267
HaeIII GGCC 1 cut(s) 56
HgaI GACGC 2 cut(s) 27, 489
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 1 cut(s) 886
HincII GTYRAC 1 cut(s) 218
HindII GTYRAC 1 cut(s) 218
HinfI GANTC 4 cut(s) 251, 508, 587, 760
HphI GGTGA 4 cut(s) 506, 517, 635, 840
Hpy166II GTNNAC 3 cut(s) 97, 218, 340
Hpy188I TCNGA 3 cut(s) 456, 538, 759
Hpy188III TCNNGA 3 cut(s) 191, 430, 806
Hpy8I GTNNAC 3 cut(s) 97, 218, 340
Hpy99I CGWCG 4 cut(s) 20, 43, 62, 505
HpyAV CCTTC 2 cut(s) 159, 786
HpyCH4III ACNGT 3 cut(s) 718, 822, 937
HpyCH4IV ACGT 3 cut(s) 18, 554, 570
HpyCH4V TGCA 3 cut(s) 51, 240, 326
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 62, 81, 246
HpyF3I CTNAG 7 cut(s) 442, 453, 537, 615, 675, 732, 756
HpySE526I ACGT 3 cut(s) 18, 554, 570
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 1 cut(s) 886
KflI GGGWCCC 1 cut(s) 751
Kzo9I GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
LmnI GCTCC 2 cut(s) 451, 874
LpnPI CCDG 9 cut(s) 176, 294, 384, 411, 546, 573, 669, 864, 976
Lsp1109I GCAGC 2 cut(s) 68, 253
LweI GCATC 2 cut(s) 74, 487
MabI ACCWGGT 1 cut(s) 559
MaeII ACGT 3 cut(s) 18, 554, 570
MaeIII GTNAC 3 cut(s) 555, 639, 720
MalI GATC 8 cut(s) 8, 260, 403, 467, 485, 520, 947, 985
MboI GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
MflI RGATCY 3 cut(s) 465, 518, 983
MluCI AATT 1 cut(s) 736
MmeI TCCRAC 2 cut(s) 183, 493
MseI TTAA 1 cut(s) 315
MspR9I CCNGG 2 cut(s) 399, 561
MvaI CCWGG 2 cut(s) 399, 561
MwoI GCNNNNNNNGC 4 cut(s) 21, 62, 81, 246
NdeII GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
NlaIII CATG 1 cut(s) 886
NlaIV GGNNCC 4 cut(s) 520, 654, 752, 753
NmuCI GTSAC 1 cut(s) 720
NspV TTCGAA 2 cut(s) 353, 479
PcsI WCGNNNNNNNCGW 1 cut(s) 270
PfeI GAWTC 4 cut(s) 251, 508, 587, 760
PkrI GCNGC 4 cut(s) 77, 80, 83, 268
PpuMI RGGWCCY 1 cut(s) 751
PshAI GACNNNNGTC 1 cut(s) 186
Psp5II RGGWCCY 1 cut(s) 751
Psp6I CCWGG 2 cut(s) 397, 559
PspGI CCWGG 2 cut(s) 397, 559
PspN4I GGNNCC 4 cut(s) 520, 654, 752, 753
PspPI GGNCC 1 cut(s) 751
PspPPI RGGWCCY 1 cut(s) 751
PsuI RGATCY 3 cut(s) 465, 518, 983
RsaI GTAC 1 cut(s) 833
RsaNI GTAC 1 cut(s) 832
SaqAI TTAA 1 cut(s) 315
SatI GCNGC 4 cut(s) 76, 79, 82, 267
Sau3AI GATC 8 cut(s) 6, 258, 401, 465, 483, 518, 945, 983
Sau96I GGNCC 1 cut(s) 751
ScrFI CCNGG 2 cut(s) 399, 561
SexAI ACCWGGT 1 cut(s) 559
SfaNI GCATC 2 cut(s) 74, 487
SfuI TTCGAA 2 cut(s) 353, 479
SinI GGWCC 1 cut(s) 751
SmlI CTYRAG 2 cut(s) 428, 804
SmoI CTYRAG 2 cut(s) 428, 804
Sse9I AATT 1 cut(s) 736
SsiI CCGC 3 cut(s) 75, 78, 492
StyD4I CCNGG 2 cut(s) 397, 559
TaaI ACNGT 3 cut(s) 718, 822, 937
TaiI ACGT 3 cut(s) 21, 557, 573
TaqI TCGA 4 cut(s) 257, 353, 479, 585
TasI AATT 1 cut(s) 736
TatI WGTACW 1 cut(s) 831
TauI GCSGC 2 cut(s) 78, 81
TfiI GAWTC 4 cut(s) 251, 508, 587, 760
Tru1I TTAA 1 cut(s) 315
Tru9I TTAA 1 cut(s) 315
TscAI CASTG 3 cut(s) 518, 723, 940
TseFI GTSAC 1 cut(s) 720
TseI GCWGC 2 cut(s) 81, 266
Tsp45I GTSAC 1 cut(s) 720
TspDTI ATGAA 4 cut(s) 17, 243, 771, 899
TspGWI ACGGA 1 cut(s) 119
TspRI CASTG 3 cut(s) 518, 723, 940
VpaK11BI GGWCC 1 cut(s) 751
XapI RAATTY 1 cut(s) 736
ZraI GACGTC 1 cut(s) 19
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.