AT1G75730

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005083
Physical Location & Seq
Reverse (-)
28435625 .. 28439835
4211 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75730.2

Sequence Viewer

Length: 1770 bp
ATGGAAATGGGTCGTCGAGATTTGATGAGACGTGGAACAACCAATCGATTTTCCAATAAACAACAATCTGGATCTGGGTTTTCTACAAAATCTAAGAAAGGAATGCTGAAGAAACTTGGTTTTGCTGATGAGAAACTGAAACAAGAGATGAGCTACGATACTCTTCCTTCTCCAGATTTAGAAAACGAGGTTTCTAAGAAGAAATCTAAGCTTCCAAAAAAGAATTTGAAAGACACTAATGGTGTGGATCATGCCTCTGTACCACGGAAGCTTCGTTCAGCCATGAAGAAGCGGAATCTTGAATCTGTTTCTAAGCTATCCTCTGTTTCAAAGAGACTTAACCGGTTTAAAACCGGAACTGAATCTTCGGTTAAGAAGGAGAGTCAAGAAATGGAAGCAAAAGCTATCGTTACTGAGTCGATGATGATTTCGAAAGATGAGAAAGAAGTTGCAGAGACTTTATATGGTTTAGCTGGAATGTTTACTGAGACTGACTCTCTTGGTAAGAAGACTAGTAATGAGAAAGAAACCAGCAAAGTTGTTGACTCAATTTTGGTTGTGGAAGATGACTATACAAAAACCGAATCGCTTACACCGGTGGTTTCAGTTCTCTCTTCAGCCAAAACTAAGCAGATTGATGCAATGCCATTAGAGCAATCTGATAAACAGTTCAACACAACAGGAATGGTTGATTTCATTGATAGATTGAAGCAAAGCAGCTCAGTGAAAGTGACTGATGCACATGCAAGAGTCAATGAAACAAAGGTTACAACAAGCAACGGTCTTGCTCTGTGGCCTGGCCTTTCTTCAACAACTGTTCATTCAGGAGCTCAAGTTCTTTCAAAGCCATCTTCAACAAAGCTTCCACCGTGGATGGGTCAAGCTGTTTCTCCTTCCAATACTGCTTCACTCTTGAACTGTGAACCATTAAGAGTCCAGCCGAGAAAGTTGAAGAGATGTGCCTCTCATATATACATTTCCCGTCTCATTAAAGTATTACAGACCAGTAAGAGTAGTCTCGCAACACTTAACCAAAACGAGCAAAGATATTCAGAAATGTCAGAACGCAGACTCCCTGATTCAGTGATAACCATTAATGACTTTAAAGCAATAGTTTCTCCACCTAAGAGGTATCAAAACCCGCATCTGCTCGATATGCACCGGGCACATAACCCAAAACCCGTGCAAGAAGACATGACTAAGCTTGCTCTCGAGCTCTATGGTCCACACACTTCACAGAAACAAAGCTTTGATTTCCTAAGTCTCTCATCTGCTGGAGCAGCACAATCTCATTTCCCACTTCCAAACTCATTTCCACAGTATCAAATCTCTGCAGCTTACAATAGCCAGCTCTCACCTGCTACTTCTAGCCATCAGGTGCAGCAGCTTTCTCCTTATCTAGCCAGTCGGTTCCAGACCGCTTACAACGCAAACCAGCAGCAGCAACAACAGCTTCAAAAAAGGCTATGGGCTGCTCAGTACAGACCAACAAATGGAAACACCATGCAGTCGAACCAATACAGTAAACCAAACCTCTCCTTAAACCTGACTAGTATTCAACAACCTCTCCAGGTTTCTTCCTCCCCAAGGTACAACCACAATGTATCTCAACAACAACATCGTCTCATGACTGCTGCTGCAGCAATGTCAATGAGCCATCAACATAATAATCCATCACGGGCGGTGATGAATAGACAAGAACACCATTTCCCACTTATATATGAAGATACAAGGACACCGTTGCAACTACTCTGCAATGAGCAATCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

589

Amino Acids

66.03

Weight (kDa)

9.75

Isoelectric Point (pI)

50.98

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1366
Acc36I ACCTGC 1 cut(s) 1366
AccB7I CCANNNNNTGG 1 cut(s) 1493
AciI CCGC 4 cut(s) 292, 1142, 1419, 1682
AclWI GGATC 2 cut(s) 79, 255
AcsI RAATTY 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 128, 600
AfaI GTAC 3 cut(s) 261, 1481, 1592
AfiI CCNNNNNNNGG 3 cut(s) 875, 1493, 1587
AgeI ACCGGT 2 cut(s) 342, 595
AhlI ACTAGT 2 cut(s) 512, 1550
AjiI CACGTC 1 cut(s) 32
AjnI CCWGG 2 cut(s) 796, 1569
AloI GAACNNNNNNTCC 8 cut(s) 259, 291, 819, 851, 1056, 1088, 1692, 1724
Alw21I GWGCWC 2 cut(s) 832, 1218
Alw26I GTCTC 8 cut(s) 22, 328, 449, 482, 989, 1022, 1268, 1628
AlwI GGATC 2 cut(s) 79, 255
Ama87I CYCGRG 1 cut(s) 1211
AoxI GGCC 2 cut(s) 794, 799
ApoI RAATTY 1 cut(s) 223
AseI ATTAAT 1 cut(s) 1095
AsiGI ACCGGT 2 cut(s) 342, 595
AspS9I GGNCC 1 cut(s) 1223
AsuC2I CCSGG 1 cut(s) 1163
AsuHPI GGTGA 2 cut(s) 1347, 1696
AsuII TTCGAA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 1211
AvaII GGWCC 1 cut(s) 1223
BaeGI GKGCMC 1 cut(s) 1168
BanII GRGCYC 2 cut(s) 832, 1218
BbsI GAAGAC 2 cut(s) 515, 1197
Bbv12I GWGCWC 2 cut(s) 832, 1218
BccI CCATC 5 cut(s) 856, 868, 1380, 1665, 1681
BciT130I CCWGG 2 cut(s) 798, 1571
BcnI CCSGG 1 cut(s) 1163
BcoDI GTCTC 8 cut(s) 22, 328, 449, 482, 989, 1022, 1268, 1628
BcuI ACTAGT 2 cut(s) 512, 1550
BfaI CTAG 4 cut(s) 513, 1368, 1400, 1551
BfmI CTRYAG 2 cut(s) 1332, 1638
BfuAI ACCTGC 1 cut(s) 1366
Bme1390I CCNGG 3 cut(s) 798, 1163, 1571
Bme18I GGWCC 1 cut(s) 1223
BmeT110I CYCGRG 1 cut(s) 1211
BmgBI CACGTC 1 cut(s) 32
BmgT120I GGNCC 1 cut(s) 1223
BmiI GGNNCC 1 cut(s) 1412
BmrFI CCNGG 3 cut(s) 798, 1163, 1571
BmsI GCATC 3 cut(s) 628, 727, 1153
BpiI GAAGAC 2 cut(s) 515, 1197
BplI GAGNNNNNCTC 2 cut(s) 479, 511
BpmI CTGGAG 3 cut(s) 156, 1296, 1553
Bpu14I TTCGAA 1 cut(s) 431
BpuEI CTTGAG 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 1163
Bsa29I ATCGAT 1 cut(s) 46
BsaJI CCNNGG 3 cut(s) 263, 869, 1586
BsaWI WCCGGW 3 cut(s) 342, 353, 595
BsaXI ACNNNNNCTCC 6 cut(s) 819, 849, 1056, 1086, 1551, 1581
Bsc4I CCNNNNNNNGG 3 cut(s) 875, 1493, 1587
Bse118I RCCGGY 2 cut(s) 342, 595
Bse1I ACTGG 2 cut(s) 1005, 1404
Bse3DI GCAATG 3 cut(s) 648, 1650, 1762
BseBI CCWGG 2 cut(s) 798, 1571
BseCI ATCGAT 1 cut(s) 46
BseDI CCNNGG 3 cut(s) 263, 869, 1586
BseGI GGATG 1 cut(s) 879
BseLI CCNNNNNNNGG 3 cut(s) 875, 1493, 1587
BseMI GCAATG 3 cut(s) 648, 1650, 1762
BseMII CTCAG 4 cut(s) 405, 477, 735, 1490
BseNI ACTGG 2 cut(s) 1005, 1404
BseSI GKGCMC 1 cut(s) 1168
BsgI GTGCAG 1 cut(s) 1400
BshFI GGCC 2 cut(s) 796, 801
BshTI ACCGGT 2 cut(s) 342, 595
BshVI ATCGAT 1 cut(s) 46
BsiHKAI GWGCWC 2 cut(s) 832, 1218
BsiHKCI CYCGRG 1 cut(s) 1211
BsiSI CCGG 4 cut(s) 343, 354, 596, 1162
BslI CCNNNNNNNGG 3 cut(s) 875, 1493, 1587
BsmAI GTCTC 8 cut(s) 22, 328, 449, 482, 989, 1022, 1268, 1628
BsmBI CGTCTC 3 cut(s) 22, 989, 1628
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 2 cut(s) 796, 801
BsoBI CYCGRG 1 cut(s) 1211
Bsp119I TTCGAA 1 cut(s) 431
Bsp1286I GDGCHC 3 cut(s) 832, 1168, 1218
Bsp143I GATC 2 cut(s) 71, 247
BspACI CCGC 4 cut(s) 292, 1142, 1419, 1682
BspANI GGCC 2 cut(s) 796, 801
BspCNI CTCAG 4 cut(s) 406, 478, 734, 1489
BspDI ATCGAT 1 cut(s) 46
BspHI TCATGA 1 cut(s) 1626
BspLI GGNNCC 1 cut(s) 1412
BspMAI CTGCAG 2 cut(s) 1336, 1642
BspMI ACCTGC 1 cut(s) 1366
BspPI GGATC 2 cut(s) 79, 255
BspT104I TTCGAA 1 cut(s) 431
BsrDI GCAATG 3 cut(s) 648, 1650, 1762
BsrFI RCCGGY 2 cut(s) 342, 595
BsrI ACTGG 2 cut(s) 1005, 1404
BssAI RCCGGY 2 cut(s) 342, 595
BssECI CCNNGG 3 cut(s) 263, 869, 1586
BssMI GATC 2 cut(s) 71, 247
BssT1I CCWWGG 1 cut(s) 1586
Bst2UI CCWGG 2 cut(s) 798, 1571
Bst4CI ACNGT 8 cut(s) 669, 782, 817, 870, 920, 1320, 1523, 1740
Bst6I CTCTTC 3 cut(s) 168, 619, 947
BstBI TTCGAA 1 cut(s) 431
BstC8I GCNNGC 2 cut(s) 1206, 1349
BstDSI CCRYGG 2 cut(s) 263, 869
BstENI CCTNNNNNAGG 1 cut(s) 1585
BstF5I GGATG 1 cut(s) 879
BstKTI GATC 2 cut(s) 74, 250
BstMAI GTCTC 8 cut(s) 22, 328, 449, 482, 989, 1022, 1268, 1628
BstMBI GATC 2 cut(s) 71, 247
BstMWI GCNNNNNNNGC 6 cut(s) 652, 1156, 1280, 1427, 1450, 1640
BstNI CCWGG 2 cut(s) 798, 1571
BstNSI RCATGY 1 cut(s) 746
BstSCI CCNGG 3 cut(s) 796, 1161, 1569
BstSFI CTRYAG 2 cut(s) 1332, 1638
BstSLI GKGCMC 1 cut(s) 1168
BstV2I GAAGAC 2 cut(s) 515, 1197
BstX2I RGATCY 1 cut(s) 71
BstYI RGATCY 1 cut(s) 71
Bsu15I ATCGAT 1 cut(s) 46
BsuRI GGCC 2 cut(s) 796, 801
BsuTUI ATCGAT 1 cut(s) 46
BtgI CCRYGG 2 cut(s) 263, 869
BtrI CACGTC 1 cut(s) 32
BtsCI GGATG 1 cut(s) 879
BtsIMutI CAGTG 2 cut(s) 729, 1089
BveI ACCTGC 1 cut(s) 1366
Cac8I GCNNGC 2 cut(s) 1206, 1349
CciI TCATGA 1 cut(s) 1626
Cfr10I RCCGGY 2 cut(s) 342, 595
Cfr13I GGNCC 1 cut(s) 1223
ClaI ATCGAT 1 cut(s) 46
Csp6I GTAC 3 cut(s) 260, 1480, 1591
CspAI ACCGGT 2 cut(s) 342, 595
CviAII CATG 6 cut(s) 251, 283, 743, 1195, 1504, 1627
CviQI GTAC 3 cut(s) 260, 1480, 1591
DpnI GATC 2 cut(s) 73, 249
DpnII GATC 2 cut(s) 71, 247
DraI TTTAAA 2 cut(s) 349, 1105
Eam1104I CTCTTC 3 cut(s) 168, 619, 947
EarI CTCTTC 3 cut(s) 168, 619, 947
Ecl136II GAGCTC 2 cut(s) 830, 1216
Eco130I CCWWGG 1 cut(s) 1586
Eco24I GRGCYC 2 cut(s) 832, 1218
Eco47I GGWCC 1 cut(s) 1223
Eco53kI GAGCTC 2 cut(s) 830, 1216
Eco57I CTGAAG 2 cut(s) 128, 600
Eco88I CYCGRG 1 cut(s) 1211
EcoICRI GAGCTC 2 cut(s) 830, 1216
EcoNI CCTNNNNNAGG 1 cut(s) 1585
EcoRII CCWGG 2 cut(s) 796, 1569
EcoT14I CCWWGG 1 cut(s) 1586
EcoT38I GRGCYC 2 cut(s) 832, 1218
ErhI CCWWGG 1 cut(s) 1586
Esp3I CGTCTC 3 cut(s) 22, 989, 1628
FaeI CATG 6 cut(s) 254, 286, 746, 1198, 1507, 1630
FatI CATG 6 cut(s) 250, 282, 742, 1194, 1503, 1626
FauI CCCGC 1 cut(s) 1149
FokI GGATG 1 cut(s) 886
FriOI GRGCYC 2 cut(s) 832, 1218
FspBI CTAG 4 cut(s) 513, 1368, 1400, 1551
GsuI CTGGAG 3 cut(s) 156, 1296, 1553
HaeIII GGCC 2 cut(s) 796, 801
HapII CCGG 4 cut(s) 343, 354, 596, 1162
Hin1II CATG 6 cut(s) 254, 286, 746, 1198, 1507, 1630
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HindIII AAGCTT 5 cut(s) 209, 269, 860, 1202, 1246
HpaII CCGG 4 cut(s) 343, 354, 596, 1162
HphI GGTGA 2 cut(s) 1347, 1696
Hpy166II GTNNAC 5 cut(s) 483, 544, 923, 1226, 1526
Hpy188I TCNGA 3 cut(s) 661, 1054, 1063
Hpy8I GTNNAC 5 cut(s) 483, 544, 923, 1226, 1526
Hpy99I CGWCG 1 cut(s) 18
HpyAV CCTTC 3 cut(s) 177, 370, 903
HpyCH4III ACNGT 8 cut(s) 669, 782, 817, 870, 920, 1320, 1523, 1740
HpyCH4IV ACGT 1 cut(s) 31
HpyF10VI GCNNNNNNNGC 6 cut(s) 652, 1156, 1280, 1427, 1450, 1640
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 6 cut(s) 254, 286, 746, 1198, 1507, 1630
Kzo9I GATC 2 cut(s) 71, 247
LmnI GCTCC 2 cut(s) 827, 1277
LweI GCATC 3 cut(s) 628, 727, 1153
MaeI CTAG 4 cut(s) 513, 1368, 1400, 1551
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 3 cut(s) 409, 730, 766
MalI GATC 2 cut(s) 73, 249
MboI GATC 2 cut(s) 71, 247
MflI RGATCY 1 cut(s) 71
MhlI GDGCHC 3 cut(s) 832, 1168, 1218
MluCI AATT 2 cut(s) 223, 549
MlyI GAGTC 7 cut(s) 391, 425, 488, 539, 759, 942, 1065
MnlI CCTC 8 cut(s) 181, 265, 331, 973, 1122, 1544, 1575, 1591
MseI TTAA 9 cut(s) 339, 348, 372, 929, 990, 1029, 1095, 1104, 1541
MspI CCGG 4 cut(s) 343, 354, 596, 1162
MspR9I CCNGG 3 cut(s) 798, 1163, 1571
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 2 cut(s) 798, 1571
MwoI GCNNNNNNNGC 6 cut(s) 652, 1156, 1280, 1427, 1450, 1640
NciI CCSGG 1 cut(s) 1163
NdeII GATC 2 cut(s) 71, 247
NlaIII CATG 6 cut(s) 254, 286, 746, 1198, 1507, 1630
NlaIV GGNNCC 1 cut(s) 1412
NmeAIII GCCGAG 1 cut(s) 966
NmuCI GTSAC 1 cut(s) 730
NspI RCATGY 1 cut(s) 746
NspV TTCGAA 1 cut(s) 431
PaeR7I CTCGAG 1 cut(s) 1211
PagI TCATGA 1 cut(s) 1626
PaqCI CACCTGC 1 cut(s) 1366
PcsI WCGNNNNNNNCGW 1 cut(s) 271
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 5 cut(s) 295, 302, 362, 584, 1079
PflMI CCANNNNNTGG 1 cut(s) 1493
PinAI ACCGGT 2 cut(s) 342, 595
PleI GAGTC 7 cut(s) 390, 424, 488, 539, 758, 941, 1065
PpsI GAGTC 7 cut(s) 390, 424, 488, 539, 758, 941, 1065
PshBI ATTAAT 1 cut(s) 1095
Psp124BI GAGCTC 2 cut(s) 832, 1218
Psp6I CCWGG 2 cut(s) 796, 1569
PspGI CCWGG 2 cut(s) 796, 1569
PspN4I GGNNCC 1 cut(s) 1412
PspPI GGNCC 1 cut(s) 1223
PstI CTGCAG 2 cut(s) 1336, 1642
PsuI RGATCY 1 cut(s) 71
RsaI GTAC 3 cut(s) 261, 1481, 1592
RsaNI GTAC 3 cut(s) 260, 1480, 1591
SacI GAGCTC 2 cut(s) 832, 1218
SaqAI TTAA 9 cut(s) 339, 348, 372, 929, 990, 1029, 1095, 1104, 1541
Sau3AI GATC 2 cut(s) 71, 247
Sau96I GGNCC 1 cut(s) 1223
SchI GAGTC 7 cut(s) 391, 425, 488, 539, 759, 942, 1065
ScrFI CCNGG 3 cut(s) 798, 1163, 1571
SduI GDGCHC 3 cut(s) 832, 1168, 1218
SfaNI GCATC 3 cut(s) 628, 727, 1153
SfcI CTRYAG 2 cut(s) 1332, 1638
Sfr274I CTCGAG 1 cut(s) 1211
SfuI TTCGAA 1 cut(s) 431
SgrAI CRCCGGYG 1 cut(s) 595
SinI GGWCC 1 cut(s) 1223
SlaI CTCGAG 1 cut(s) 1211
SmlI CTYRAG 2 cut(s) 831, 1211
SmoI CTYRAG 2 cut(s) 831, 1211
SpeI ACTAGT 2 cut(s) 512, 1550
Sse9I AATT 2 cut(s) 223, 549
SsiI CCGC 4 cut(s) 292, 1142, 1419, 1682
SspMI CTAG 4 cut(s) 513, 1368, 1400, 1551
SstI GAGCTC 2 cut(s) 832, 1218
StyD4I CCNGG 3 cut(s) 796, 1161, 1569
StyI CCWWGG 1 cut(s) 1586
TaaI ACNGT 8 cut(s) 669, 782, 817, 870, 920, 1320, 1523, 1740
TaiI ACGT 1 cut(s) 34
TaqI TCGA 7 cut(s) 16, 46, 419, 431, 1152, 1212, 1511
TasI AATT 2 cut(s) 223, 549
TatI WGTACW 1 cut(s) 1479
TfiI GAWTC 5 cut(s) 295, 302, 362, 584, 1079
Tru1I TTAA 9 cut(s) 339, 348, 372, 929, 990, 1029, 1095, 1104, 1541
Tru9I TTAA 9 cut(s) 339, 348, 372, 929, 990, 1029, 1095, 1104, 1541
TscAI CASTG 2 cut(s) 729, 1089
TseFI GTSAC 1 cut(s) 730
Tsp45I GTSAC 1 cut(s) 730
TspDTI ATGAA 6 cut(s) 299, 685, 771, 809, 1703, 1737
TspGWI ACGGA 1 cut(s) 280
TspRI CASTG 2 cut(s) 729, 1089
Van91I CCANNNNNTGG 1 cut(s) 1493
VpaK11BI GGWCC 1 cut(s) 1223
VspI ATTAAT 1 cut(s) 1095
XagI CCTNNNNNAGG 1 cut(s) 1585
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 746
XhoI CTCGAG 1 cut(s) 1211
XspI CTAG 4 cut(s) 513, 1368, 1400, 1551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.