Rorug06G0317300

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0005083
Physical Location & Seq
Reverse (-)
46898718 .. 46901483
2766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0317300.1

Sequence Viewer

Length: 1191 bp
ATGAAAATACAGCCGATTGAGTCACACGCGGGGCCTGTGGAAGCGGCGACTAGGTTCGAACCGGTGAAGCCGGTGGCCAAGTCACGGTTCAAGAGGCTCTTCGAGCGACAGTTCACGAGCGTATTGAGAACAACGACCCCTGCAGCGGCGGCGGCGGAGGAGAAGGTCGTCGTCTGGGGTGTCGAGGAGCTGCATTTCAACAAAGACGGCTTCACGGACTTGGCCGGCGGCGAGTTCGAGCCGAGCTCAGTCTGCTTGGCGAATATGGTGCAGAACTTCATCGAAGAGAGCAACGAGAAGCAATCGTCGGCCAGGTGCAGCCGGAACCGCTGTAACTGCTTCAACGGAAACTGCGACGACAGCTCCGACGACGAATCGGAGCCTTTCGGTTTCGGCGATTCCAATCTGGCTTCATCCGGCGAAGCATGTGAGCTTCTCAAGAGTTTGGTGTCGTGCCCAAGTCTATGCGAAAGGAATTTGCTAGCGGACACAGCTAGAATCGTCGAGAAAAACAAGATGTGCAAGCGTAAAGACGACAATGGCAGACTGGTTGTTACTGATAATCTGTTAGCTCTCGGATACGACGCTTCAGTCTGCAAAACTCGTTGGGAAAAATCCCCTTCCTATCCTTCCGGGGAGTACGAGTACATTGACGTGATCGTAAATGGGGAGAGGCTGCTAATCGACGTGGATTTCCGATCGGAGTTTGAGATTGCTCGACCGACGAAGAGCTACAAGGCGATTCTGCAGGCTCTTCCTTATATTTTCGTTGGGAAGCCCGATCGGCTGAGGAGGATCATCGGAATTGTTTCGGAGGCGGCGAAGCAGAGCCTGAAGAAGAAGGGAATGCACATTCCGCCGTGGAGGAAAGCAGAGTACATCAAAGCCAAGTGGCTCTCTCCTCACACCCGCGCCTCAATCTCCGTCAATTCCTCACCGGATTCCTTGCCTGAAAAGACCGAGACCCAGGCTTTGATCAAGTCCATGGAGTTTGAACAGAGTCGTACCGAGTTGGCAAAGTCGGTGGACTCTTCTGAGCCGGAGGAGTCGGTTTTTCAGTTGTCTGAGGGAGAAGAGGAGAAGGCGGTGGTGAAGGAGTGGAAGCCGCCGGAGGTGAAGCCCAGGAGATCATCAGGGGTGAAGATTGTGACTGGTTTGGCTTCAGTCATGGAAGATGAAAATGAGCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

396

Amino Acids

44.21

Weight (kDa)

5.52

Isoelectric Point (pI)

58.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PDDEXK_6 PF04720 88 - 304 2.2e-76 PDDEXK-like family of unknown function
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 590
AccII CGCG 2 cut(s) 29, 912
AclWI GGATC 1 cut(s) 803
AcoI YGGCCR 3 cut(s) 75, 222, 309
AcsI RAATTY 1 cut(s) 475
AcuI CTGAAG 3 cut(s) 573, 854, 1146
AfaI GTAC 4 cut(s) 641, 647, 878, 1006
AfiI CCNNNNNNNGG 2 cut(s) 84, 145
AgeI ACCGGT 1 cut(s) 61
AgsI TTSAA 4 cut(s) 91, 199, 343, 995
AjiI CACGTC 2 cut(s) 655, 688
AjnI CCWGG 3 cut(s) 311, 966, 1121
AjuI GAANNNNNNNTTGG 2 cut(s) 71, 103
AluBI AGCT 7 cut(s) 190, 246, 363, 433, 494, 572, 732
AluI AGCT 7 cut(s) 190, 246, 363, 433, 494, 572, 732
Alw21I GWGCWC 1 cut(s) 248
Alw26I GTCTC 1 cut(s) 956
AlwI GGATC 1 cut(s) 803
AlwNI CAGNNNCTG 1 cut(s) 832
AoxI GGCC 4 cut(s) 32, 75, 222, 309
ApeKI GCWGC 4 cut(s) 143, 190, 318, 676
ApoI RAATTY 1 cut(s) 475
ArsI GACNNNNNNTTYG 2 cut(s) 955, 987
AsiGI ACCGGT 1 cut(s) 61
Asp700I GAANNNNTTC 1 cut(s) 808
AspLEI GCGC 1 cut(s) 914
AspS9I GGNCC 1 cut(s) 32
AsuC2I CCSGG 1 cut(s) 634
AsuHPI GGTGA 5 cut(s) 76, 927, 1102, 1126, 1150
AsuII TTCGAA 1 cut(s) 57
AsuNHI GCTAGC 1 cut(s) 481
BaeGI GKGCMC 1 cut(s) 458
BalI TGGCCA 1 cut(s) 77
BanII GRGCYC 1 cut(s) 248
BauI CACGAG 1 cut(s) 115
Bbv12I GWGCWC 1 cut(s) 248
BbvCI CCTCAGC 1 cut(s) 788
BbvI GCAGC 4 cut(s) 155, 177, 330, 663
BceAI ACGGC 2 cut(s) 223, 844
BcgI CGANNNNNNTGC 2 cut(s) 250, 284
BciT130I CCWGG 3 cut(s) 313, 968, 1123
BciVI GTATCC 1 cut(s) 572
BclI TGATCA 1 cut(s) 975
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 956
BfaI CTAG 3 cut(s) 51, 482, 495
BfmI CTRYAG 2 cut(s) 141, 746
BfuI GTATCC 1 cut(s) 572
BglI GCCNNNNNGGC 1 cut(s) 784
Bme1390I CCNGG 4 cut(s) 313, 634, 968, 1123
BmgBI CACGTC 2 cut(s) 655, 688
BmgT120I GGNCC 1 cut(s) 32
BmiI GGNNCC 3 cut(s) 33, 326, 381
BmrFI CCNGG 4 cut(s) 313, 634, 968, 1123
BmtI GCTAGC 1 cut(s) 485
BplI GAGNNNNNCTC 2 cut(s) 230, 262
Bpu10I CCTNAGC 1 cut(s) 788
Bpu14I TTCGAA 1 cut(s) 57
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 634
BsaBI GATNNNNATC 1 cut(s) 402
BsaI GGTCTC 1 cut(s) 956
BsaJI CCNNGG 5 cut(s) 633, 860, 966, 984, 1121
BsaWI WCCGGW 2 cut(s) 61, 937
BsaXI ACNNNNNCTCC 4 cut(s) 347, 377, 629, 659
Bsc4I CCNNNNNNNGG 2 cut(s) 84, 145
Bse118I RCCGGY 3 cut(s) 61, 70, 224
Bse1I ACTGG 2 cut(s) 552, 1156
Bse8I GATNNNNATC 1 cut(s) 402
BseBI CCWGG 3 cut(s) 313, 968, 1123
BseDI CCNNGG 5 cut(s) 633, 860, 966, 984, 1121
BseGI GGATG 1 cut(s) 413
BseJI GATNNNNATC 1 cut(s) 402
BseLI CCNNNNNNNGG 2 cut(s) 84, 145
BseMII CTCAG 4 cut(s) 261, 779, 1026, 1056
BseNI ACTGG 2 cut(s) 552, 1156
BseRI GAGGAG 6 cut(s) 173, 200, 805, 891, 1058, 1091
BseSI GKGCMC 1 cut(s) 458
BseXI GCAGC 4 cut(s) 155, 177, 330, 663
BsgI GTGCAG 2 cut(s) 290, 337
Bsh1236I CGCG 2 cut(s) 29, 912
Bsh1285I CGRYCG 3 cut(s) 701, 722, 784
BshFI GGCC 4 cut(s) 34, 77, 224, 311
BshTI ACCGGT 1 cut(s) 61
BsiEI CGRYCG 3 cut(s) 701, 722, 784
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 9 cut(s) 62, 71, 225, 322, 417, 633, 938, 1040, 1109
BslI CCNNNNNNNGG 2 cut(s) 84, 145
BsmAI GTCTC 1 cut(s) 956
BsmI GAATGC 1 cut(s) 852
BsnI GGCC 4 cut(s) 34, 77, 224, 311
Bso31I GGTCTC 1 cut(s) 956
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 2 cut(s) 248, 458
Bsp143I GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
Bsp19I CCATGG 1 cut(s) 984
BspANI GGCC 4 cut(s) 34, 77, 224, 311
BspCNI CTCAG 4 cut(s) 260, 780, 1027, 1057
BspFNI CGCG 2 cut(s) 29, 912
BspLI GGNNCC 3 cut(s) 33, 326, 381
BspMAI CTGCAG 2 cut(s) 145, 750
BspOI GCTAGC 1 cut(s) 485
BspPI GGATC 1 cut(s) 803
BspQI GCTCTTC 3 cut(s) 104, 722, 759
BspT104I TTCGAA 1 cut(s) 57
BspTNI GGTCTC 1 cut(s) 956
BsrFI RCCGGY 3 cut(s) 61, 70, 224
BsrI ACTGG 2 cut(s) 552, 1156
BssAI RCCGGY 3 cut(s) 61, 70, 224
BssECI CCNNGG 5 cut(s) 633, 860, 966, 984, 1121
BssMI GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
BssSI CACGAG 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 984
Bst2BI CACGAG 1 cut(s) 115
Bst2UI CCWGG 3 cut(s) 313, 968, 1123
Bst4CI ACNGT 2 cut(s) 87, 111
Bst6I CTCTTC 6 cut(s) 104, 279, 722, 759, 1036, 1068
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 4 cut(s) 226, 483, 524, 750
BstDEI CTNAG 4 cut(s) 247, 788, 1035, 1065
BstDSI CCRYGG 2 cut(s) 860, 984
BstF5I GGATG 1 cut(s) 413
BstFNI CGCG 2 cut(s) 29, 912
BstHHI GCGC 1 cut(s) 914
BstKTI GATC 6 cut(s) 660, 701, 784, 798, 978, 1130
BstMAI GTCTC 1 cut(s) 956
BstMBI GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
BstMCI CGRYCG 3 cut(s) 701, 722, 784
BstNI CCWGG 3 cut(s) 313, 968, 1123
BstNSI RCATGY 1 cut(s) 429
BstSCI CCNGG 4 cut(s) 311, 632, 966, 1121
BstSFI CTRYAG 2 cut(s) 141, 746
BstSLI GKGCMC 1 cut(s) 458
BstUI CGCG 2 cut(s) 29, 912
BstV1I GCAGC 4 cut(s) 155, 177, 330, 663
BsuI GTATCC 1 cut(s) 572
BsuRI GGCC 4 cut(s) 34, 77, 224, 311
BtgI CCRYGG 2 cut(s) 860, 984
BtrI CACGTC 2 cut(s) 655, 688
BtsCI GGATG 1 cut(s) 413
Cac8I GCNNGC 4 cut(s) 226, 483, 524, 750
CaiI CAGNNNCTG 1 cut(s) 832
CfoI GCGC 1 cut(s) 914
Cfr10I RCCGGY 3 cut(s) 61, 70, 224
Cfr13I GGNCC 1 cut(s) 32
CseI GACGC 1 cut(s) 593
Csp6I GTAC 4 cut(s) 640, 646, 877, 1005
CspAI ACCGGT 1 cut(s) 61
CspCI CAANNNNNGTGG 2 cut(s) 1005, 1040
CviAII CATG 4 cut(s) 426, 985, 1168, 1188
CviQI GTAC 4 cut(s) 640, 646, 877, 1005
DdeI CTNAG 4 cut(s) 247, 788, 1035, 1065
DpnI GATC 6 cut(s) 659, 700, 783, 797, 977, 1129
DpnII GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 3 cut(s) 75, 222, 309
Eam1104I CTCTTC 6 cut(s) 104, 279, 722, 759, 1036, 1068
EarI CTCTTC 6 cut(s) 104, 279, 722, 759, 1036, 1068
EciI GGCGGA 2 cut(s) 170, 846
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 984
Eco24I GRGCYC 1 cut(s) 248
Eco31I GGTCTC 1 cut(s) 956
Eco53kI GAGCTC 1 cut(s) 246
Eco57I CTGAAG 3 cut(s) 573, 854, 1146
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 32
EcoRII CCWGG 3 cut(s) 311, 966, 1121
EcoT14I CCWWGG 1 cut(s) 984
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 984
FaeI CATG 4 cut(s) 429, 988, 1171, 1191
FaiI YATR 7 cut(s) 266, 427, 466, 762, 986, 1169, 1189
FalI AAGNNNNNCTT 2 cut(s) 83, 115
FatI CATG 4 cut(s) 425, 984, 1167, 1187
FauI CCCGC 2 cut(s) 22, 917
FbaI TGATCA 1 cut(s) 975
FokI GGATG 1 cut(s) 400
FriOI GRGCYC 1 cut(s) 248
FspBI CTAG 3 cut(s) 51, 482, 495
GlaI GCGC 1 cut(s) 913
HaeIII GGCC 4 cut(s) 34, 77, 224, 311
HapII CCGG 9 cut(s) 62, 71, 225, 322, 417, 633, 938, 1040, 1109
HgaI GACGC 1 cut(s) 593
HhaI GCGC 1 cut(s) 914
Hin1II CATG 4 cut(s) 429, 988, 1171, 1191
Hin6I GCGC 1 cut(s) 912
HinP1I GCGC 1 cut(s) 912
HinfI GANTC 9 cut(s) 20, 374, 398, 498, 742, 941, 1000, 1028, 1046
HpaII CCGG 9 cut(s) 62, 71, 225, 322, 417, 633, 938, 1040, 1109
HphI GGTGA 5 cut(s) 76, 927, 1102, 1126, 1150
Hpy166II GTNNAC 2 cut(s) 114, 1027
Hpy188I TCNGA 9 cut(s) 367, 379, 578, 698, 703, 803, 814, 1036, 1066
Hpy188III TCNNGA 4 cut(s) 91, 115, 439, 505
Hpy8I GTNNAC 2 cut(s) 114, 1027
Hpy99I CGWCG 9 cut(s) 173, 310, 359, 371, 374, 506, 587, 689, 727
HpyAV CCTTC 6 cut(s) 157, 630, 639, 835, 1075, 1087
HpyCH4III ACNGT 2 cut(s) 87, 111
HpyCH4IV ACGT 2 cut(s) 654, 687
HpyCH4V TGCA 8 cut(s) 143, 193, 271, 318, 522, 597, 748, 850
HpyF3I CTNAG 4 cut(s) 247, 788, 1035, 1065
HpySE526I ACGT 2 cut(s) 654, 687
Hsp92II CATG 4 cut(s) 429, 988, 1171, 1191
HspAI GCGC 1 cut(s) 912
KroI GCCGGC 1 cut(s) 224
KroNI GCCGGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 975
Kzo9I GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
LguI GCTCTTC 3 cut(s) 104, 722, 759
LmnI GCTCC 3 cut(s) 187, 368, 379
Lsp1109I GCAGC 4 cut(s) 155, 177, 330, 663
MaeI CTAG 3 cut(s) 51, 482, 495
MaeII ACGT 2 cut(s) 654, 687
MaeIII GTNAC 5 cut(s) 21, 81, 332, 553, 1147
MalI GATC 6 cut(s) 659, 700, 783, 797, 977, 1129
MboI GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
MhlI GDGCHC 2 cut(s) 248, 458
MlsI TGGCCA 1 cut(s) 77
MluCI AATT 3 cut(s) 475, 804, 928
MluNI TGGCCA 1 cut(s) 77
MlyI GAGTC 4 cut(s) 29, 1009, 1022, 1055
MmeI TCCRAC 1 cut(s) 390
Mox20I TGGCCA 1 cut(s) 77
MroNI GCCGGC 1 cut(s) 224
MroXI GAANNNNTTC 1 cut(s) 808
MscI TGGCCA 1 cut(s) 77
MslI CAYNNNNRTG 1 cut(s) 653
Msp20I TGGCCA 1 cut(s) 77
MspA1I CMGCKG 2 cut(s) 146, 330
MspI CCGG 9 cut(s) 62, 71, 225, 322, 417, 633, 938, 1040, 1109
MspR9I CCNGG 4 cut(s) 313, 634, 968, 1123
Mva1269I GAATGC 1 cut(s) 852
MvaI CCWGG 3 cut(s) 313, 968, 1123
MvnI CGCG 2 cut(s) 29, 912
NaeI GCCGGC 1 cut(s) 226
NciI CCSGG 1 cut(s) 634
NcoI CCATGG 1 cut(s) 984
NdeII GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
NgoMIV GCCGGC 1 cut(s) 224
NheI GCTAGC 1 cut(s) 481
NlaIII CATG 4 cut(s) 429, 988, 1171, 1191
NlaIV GGNNCC 3 cut(s) 33, 326, 381
NmeAIII GCCGAG 1 cut(s) 267
NmuCI GTSAC 3 cut(s) 21, 81, 1147
NspI RCATGY 1 cut(s) 429
NspV TTCGAA 1 cut(s) 57
PciSI GCTCTTC 3 cut(s) 104, 722, 759
PcsI WCGNNNNNNNCGW 4 cut(s) 351, 363, 393, 818
PctI GAATGC 1 cut(s) 852
PdiI GCCGGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 5 cut(s) 374, 398, 498, 742, 941
PinAI ACCGGT 1 cut(s) 61
Ple19I CGATCG 2 cut(s) 701, 784
PleI GAGTC 4 cut(s) 28, 1008, 1022, 1054
PpsI GAGTC 4 cut(s) 28, 1008, 1022, 1054
Psp124BI GAGCTC 1 cut(s) 248
Psp6I CCWGG 3 cut(s) 311, 966, 1121
PspGI CCWGG 3 cut(s) 311, 966, 1121
PspN4I GGNNCC 3 cut(s) 33, 326, 381
PspPI GGNCC 1 cut(s) 32
PstI CTGCAG 2 cut(s) 145, 750
PstNI CAGNNNCTG 1 cut(s) 832
PvuI CGATCG 2 cut(s) 701, 784
RsaI GTAC 4 cut(s) 641, 647, 878, 1006
RsaNI GTAC 4 cut(s) 640, 646, 877, 1005
RseI CAYNNNNRTG 1 cut(s) 653
SacI GAGCTC 1 cut(s) 248
SapI GCTCTTC 3 cut(s) 104, 722, 759
Sau3AI GATC 6 cut(s) 657, 698, 781, 795, 975, 1127
Sau96I GGNCC 1 cut(s) 32
SchI GAGTC 4 cut(s) 29, 1009, 1022, 1055
ScrFI CCNGG 4 cut(s) 313, 634, 968, 1123
SduI GDGCHC 2 cut(s) 248, 458
SfcI CTRYAG 2 cut(s) 141, 746
SfuI TTCGAA 1 cut(s) 57
SmiMI CAYNNNNRTG 1 cut(s) 653
SmlI CTYRAG 1 cut(s) 437
SmoI CTYRAG 1 cut(s) 437
Sse9I AATT 3 cut(s) 475, 804, 928
SspMI CTAG 3 cut(s) 51, 482, 495
SstI GAGCTC 1 cut(s) 248
StyD4I CCNGG 4 cut(s) 311, 632, 966, 1121
StyI CCWWGG 1 cut(s) 984
TaaI ACNGT 2 cut(s) 87, 111
TaiI ACGT 2 cut(s) 657, 690
TaqI TCGA 8 cut(s) 57, 102, 183, 237, 282, 504, 684, 718
TaqII GACCGA 2 cut(s) 736, 974
TasI AATT 3 cut(s) 475, 804, 928
TatI WGTACW 2 cut(s) 645, 876
TauI GCSGC 7 cut(s) 47, 149, 152, 155, 231, 821, 1108
TfiI GAWTC 5 cut(s) 374, 398, 498, 742, 941
TseFI GTSAC 3 cut(s) 21, 81, 1147
TseI GCWGC 4 cut(s) 143, 190, 318, 676
Tsp45I GTSAC 3 cut(s) 21, 81, 1147
TspDTI ATGAA 4 cut(s) 17, 268, 402, 1191
TspGWI ACGGA 3 cut(s) 230, 360, 913
XapI RAATTY 1 cut(s) 475
XceI RCATGY 1 cut(s) 429
XmnI GAANNNNTTC 1 cut(s) 808
XspI CTAG 3 cut(s) 51, 482, 495
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.