AT1G76450

PsbP

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0009272
Physical Location & Seq
Forward (+)
28684551 .. 28686244
1694 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G76450.1

Sequence Viewer

Length: 744 bp
ATGGCAGCGATTTCTCCATGGTTATCTTCTCCTCAGAGCTTTTCGAATCCCCGCGTTACCATTACAGATTCCAGAAGATGTTCATCAATTTCTGCGGCAATCTCTGTTCTTGACAGCTCCAACGAGGAACAACATCGAATTTCGTCTAGAGATCATGTTGGGATGAAGAGAAGAGACGTCATGTTACAGATAGCTTCCTCTGTTTTCTTCCTTCCATTGGCCATTTCACCTGCATTTGCAGAGACAAATGCATCAGAAGCTTTCCGTGTGTACACAGATGAAACGAACAAATTCGAGATATCAATCCCACAAGATTGGCAAGTCGGGCAAGCAGAACCTAATGGATTCAAGTCAATCACAGCTTTTTACCCACAAGAAACTTCAACTTCCAATGTGAGTATAGCGATCACTGGACTAGGTCCAGACTTCACCAGGATGGAATCATTCGGAAAGGTCGAAGCTTTCGCCGAAACATTGGTCAGTGGATTGGATAGAAGCTGGCAAAAACCAGTAGGAGTGACTGCAAAGCTAATCGATAGCAGAGCTTCTAAGGGATTCTATTACATCGAGTACACCTTACAAAACCCTGGAGAAGCTCGCAAGCATTTGTACTCTGCAATTGGAATGGCAACAAACGGATGGTACAACCGTTTATACACTGTCACAGGACAGTTTACAGATGAAGAATCTGCTGAACAAAGCTCTAAGATCCAGAAGACAGTCAAGTCTTTCAGATTCATCTGA

Protein Analysis

247

Amino Acids

27.49

Weight (kDa)

6.33

Isoelectric Point (pI)

48.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 87 - 246 3.7e-30 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 238
AasI GACNNNNNNGTC 1 cut(s) 724
AatII GACGTC 1 cut(s) 180
Acc36I ACCTGC 1 cut(s) 238
AccII CGCG 1 cut(s) 54
AciI CCGC 2 cut(s) 52, 95
AclWI GGATC 1 cut(s) 703
AcoI YGGCCR 1 cut(s) 219
AcsI RAATTY 2 cut(s) 138, 290
AcyI GRCGYC 1 cut(s) 177
AfaI GTAC 4 cut(s) 272, 572, 611, 644
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 2 cut(s) 349, 384
AjnI CCWGG 2 cut(s) 431, 586
Alw26I GTCTC 2 cut(s) 168, 236
AlwI GGATC 1 cut(s) 703
AoxI GGCC 1 cut(s) 219
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 2 cut(s) 138, 290
ArsI GACNNNNNNTTYG 2 cut(s) 462, 494
Asp700I GAANNNNTTC 2 cut(s) 79, 290
AspS9I GGNCC 1 cut(s) 419
AsuHPI GGTGA 2 cut(s) 219, 421
AsuII TTCGAA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 419
BaeI ACNNNNGTAYC 2 cut(s) 634, 667
BalI TGGCCA 1 cut(s) 221
BbsI GAAGAC 1 cut(s) 722
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 430, 633
BciT130I CCWGG 2 cut(s) 433, 588
BcoDI GTCTC 2 cut(s) 168, 236
BfaI CTAG 2 cut(s) 147, 416
BfuAI ACCTGC 1 cut(s) 238
BisI GCNGC 2 cut(s) 6, 96
BlsI GCNGC 2 cut(s) 7, 97
Bme1390I CCNGG 2 cut(s) 433, 588
Bme18I GGWCC 1 cut(s) 419
BmgT120I GGNCC 1 cut(s) 419
BmrFI CCNGG 2 cut(s) 433, 588
BmsI GCATC 1 cut(s) 260
BpiI GAAGAC 1 cut(s) 722
BpmI CTGGAG 1 cut(s) 609
Bpu14I TTCGAA 1 cut(s) 44
Bsa29I ATCGAT 1 cut(s) 534
BsaBI GATNNNNATC 2 cut(s) 82, 302
BsaHI GRCGYC 1 cut(s) 177
BsaJI CCNNGG 2 cut(s) 17, 586
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse1I ACTGG 2 cut(s) 415, 509
Bse8I GATNNNNATC 2 cut(s) 82, 302
BseBI CCWGG 2 cut(s) 433, 588
BseCI ATCGAT 1 cut(s) 534
BseDI CCNNGG 2 cut(s) 17, 586
BseGI GGATG 3 cut(s) 168, 441, 644
BseJI GATNNNNATC 2 cut(s) 82, 302
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMII CTCAG 1 cut(s) 47
BseNI ACTGG 2 cut(s) 415, 509
BseRI GAGGAG 1 cut(s) 21
BseXI GCAGC 1 cut(s) 17
Bsh1236I CGCG 1 cut(s) 54
BshFI GGCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 534
BslI CCNNNNNNNGG 1 cut(s) 217
BsmAI GTCTC 2 cut(s) 168, 236
BsmBI CGTCTC 1 cut(s) 168
BsnI GGCC 1 cut(s) 221
Bsp119I TTCGAA 1 cut(s) 44
Bsp1407I TGTACA 1 cut(s) 270
Bsp143I GATC 3 cut(s) 151, 405, 708
Bsp19I CCATGG 1 cut(s) 17
BspACI CCGC 2 cut(s) 52, 95
BspANI GGCC 1 cut(s) 221
BspCNI CTCAG 1 cut(s) 46
BspDI ATCGAT 1 cut(s) 534
BspFNI CGCG 1 cut(s) 54
BspMI ACCTGC 1 cut(s) 238
BspPI GGATC 1 cut(s) 703
BspT104I TTCGAA 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 270
BsrI ACTGG 2 cut(s) 415, 509
BssECI CCNNGG 2 cut(s) 17, 586
BssMI GATC 3 cut(s) 151, 405, 708
BssNI GRCGYC 1 cut(s) 177
BssT1I CCWWGG 1 cut(s) 17
Bst2UI CCWGG 2 cut(s) 433, 588
Bst4CI ACNGT 4 cut(s) 650, 661, 672, 721
Bst6I CTCTTC 2 cut(s) 161, 166
BstACI GRCGYC 1 cut(s) 177
BstAUI TGTACA 1 cut(s) 270
BstBI TTCGAA 1 cut(s) 44
BstC8I GCNNGC 4 cut(s) 330, 500, 598, 602
BstDEI CTNAG 3 cut(s) 33, 549, 705
BstDSI CCRYGG 1 cut(s) 17
BstF5I GGATG 3 cut(s) 168, 441, 644
BstFNI CGCG 1 cut(s) 54
BstKTI GATC 3 cut(s) 154, 408, 711
BstMAI GTCTC 2 cut(s) 168, 236
BstMBI GATC 3 cut(s) 151, 405, 708
BstMWI GCNNNNNNNGC 2 cut(s) 257, 325
BstNI CCWGG 2 cut(s) 433, 588
BstSCI CCNGG 2 cut(s) 431, 586
BstUI CGCG 1 cut(s) 54
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 1 cut(s) 722
BstX2I RGATCY 1 cut(s) 708
BstXI CCANNNNNNTGG 1 cut(s) 315
BstYI RGATCY 1 cut(s) 708
Bsu15I ATCGAT 1 cut(s) 534
BsuRI GGCC 1 cut(s) 221
BsuTUI ATCGAT 1 cut(s) 534
BtgI CCRYGG 1 cut(s) 17
BtsCI GGATG 3 cut(s) 168, 441, 644
BtsIMutI CAGTG 3 cut(s) 408, 487, 657
BveI ACCTGC 1 cut(s) 238
Cac8I GCNNGC 4 cut(s) 330, 500, 598, 602
Cfr13I GGNCC 1 cut(s) 419
ClaI ATCGAT 1 cut(s) 534
Csp6I GTAC 4 cut(s) 271, 571, 610, 643
CviAII CATG 3 cut(s) 18, 155, 181
CviQI GTAC 4 cut(s) 271, 571, 610, 643
DdeI CTNAG 3 cut(s) 33, 549, 705
DpnI GATC 3 cut(s) 153, 407, 710
DpnII GATC 3 cut(s) 151, 405, 708
DrdI GACNNNNNNGTC 1 cut(s) 724
DseDI GACNNNNNNGTC 1 cut(s) 724
EaeI YGGCCR 1 cut(s) 219
Eam1104I CTCTTC 2 cut(s) 161, 166
EarI CTCTTC 2 cut(s) 161, 166
Eco130I CCWWGG 1 cut(s) 17
Eco32I GATATC 1 cut(s) 300
Eco47I GGWCC 1 cut(s) 419
EcoRII CCWGG 2 cut(s) 431, 586
EcoRV GATATC 1 cut(s) 300
EcoT14I CCWWGG 1 cut(s) 17
EcoT22I ATGCAT 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 17
Esp3I CGTCTC 1 cut(s) 168
FaeI CATG 3 cut(s) 21, 158, 184
FaiI YATR 5 cut(s) 19, 156, 182, 401, 655
FatI CATG 3 cut(s) 17, 154, 180
FauI CCCGC 1 cut(s) 59
Fnu4HI GCNGC 2 cut(s) 6, 96
FokI GGATG 3 cut(s) 175, 448, 651
Fsp4HI GCNGC 2 cut(s) 6, 96
FspBI CTAG 2 cut(s) 147, 416
GluI GCNGC 2 cut(s) 6, 96
GsuI CTGGAG 1 cut(s) 609
HaeIII GGCC 1 cut(s) 221
Hin1I GRCGYC 1 cut(s) 177
Hin1II CATG 3 cut(s) 21, 158, 184
HindIII AAGCTT 2 cut(s) 258, 459
HinfI GANTC 7 cut(s) 46, 68, 345, 440, 555, 686, 735
HphI GGTGA 2 cut(s) 219, 421
Hpy166II GTNNAC 4 cut(s) 271, 273, 573, 675
Hpy188I TCNGA 5 cut(s) 36, 256, 449, 734, 743
Hpy188III TCNNGA 6 cut(s) 72, 110, 147, 295, 422, 712
Hpy8I GTNNAC 4 cut(s) 271, 273, 573, 675
HpyAV CCTTC 1 cut(s) 221
HpyCH4III ACNGT 4 cut(s) 650, 661, 672, 721
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 5 cut(s) 233, 239, 251, 524, 617
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 325
HpyF3I CTNAG 3 cut(s) 33, 549, 705
HpySE526I ACGT 1 cut(s) 177
Hsp92I GRCGYC 1 cut(s) 177
Hsp92II CATG 3 cut(s) 21, 158, 184
Kzo9I GATC 3 cut(s) 151, 405, 708
LmnI GCTCC 1 cut(s) 122
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 260
MaeI CTAG 2 cut(s) 147, 416
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 4 cut(s) 55, 183, 517, 661
MalI GATC 3 cut(s) 153, 407, 710
MboI GATC 3 cut(s) 151, 405, 708
MboII GAAGA 7 cut(s) 18, 87, 178, 183, 199, 695, 727
MfeI CAATTG 1 cut(s) 618
MflI RGATCY 1 cut(s) 708
MlsI TGGCCA 1 cut(s) 221
MluCI AATT 4 cut(s) 87, 138, 290, 618
MluNI TGGCCA 1 cut(s) 221
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 3 cut(s) 42, 118, 208
Mox20I TGGCCA 1 cut(s) 221
Mph1103I ATGCAT 1 cut(s) 253
MroXI GAANNNNTTC 2 cut(s) 79, 290
MscI TGGCCA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 434
Msp20I TGGCCA 1 cut(s) 221
MspR9I CCNGG 2 cut(s) 433, 588
MunI CAATTG 1 cut(s) 618
MvaI CCWGG 2 cut(s) 433, 588
MvnI CGCG 1 cut(s) 54
MwoI GCNNNNNNNGC 2 cut(s) 257, 325
NcoI CCATGG 1 cut(s) 17
NdeII GATC 3 cut(s) 151, 405, 708
NlaIII CATG 3 cut(s) 21, 158, 184
NmuCI GTSAC 2 cut(s) 517, 661
NsiI ATGCAT 1 cut(s) 253
NspV TTCGAA 1 cut(s) 44
PaqCI CACCTGC 1 cut(s) 238
PcsI WCGNNNNNNNCGW 1 cut(s) 453
PdmI GAANNNNTTC 2 cut(s) 79, 290
PfeI GAWTC 7 cut(s) 46, 68, 345, 440, 555, 686, 735
PflFI GACNNNGTC 1 cut(s) 417
PkrI GCNGC 2 cut(s) 7, 97
Psp6I CCWGG 2 cut(s) 431, 586
PspGI CCWGG 2 cut(s) 431, 586
PspPI GGNCC 1 cut(s) 419
PsuI RGATCY 1 cut(s) 708
PsyI GACNNNGTC 1 cut(s) 417
RsaI GTAC 4 cut(s) 272, 572, 611, 644
RsaNI GTAC 4 cut(s) 271, 571, 610, 643
RseI CAYNNNNRTG 1 cut(s) 434
SatI GCNGC 2 cut(s) 6, 96
Sau3AI GATC 3 cut(s) 151, 405, 708
Sau96I GGNCC 1 cut(s) 419
ScrFI CCNGG 2 cut(s) 433, 588
SfaNI GCATC 1 cut(s) 260
SfuI TTCGAA 1 cut(s) 44
SinI GGWCC 1 cut(s) 419
SmiMI CAYNNNNRTG 1 cut(s) 434
Sse9I AATT 4 cut(s) 87, 138, 290, 618
SsiI CCGC 2 cut(s) 52, 95
SspMI CTAG 2 cut(s) 147, 416
StyD4I CCNGG 2 cut(s) 431, 586
StyI CCWWGG 1 cut(s) 17
TaaI ACNGT 4 cut(s) 650, 661, 672, 721
TaiI ACGT 1 cut(s) 180
TaqI TCGA 6 cut(s) 44, 136, 294, 456, 534, 567
TasI AATT 4 cut(s) 87, 138, 290, 618
TatI WGTACW 3 cut(s) 270, 570, 609
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 7 cut(s) 46, 68, 345, 440, 555, 686, 735
TscAI CASTG 3 cut(s) 415, 487, 664
TseFI GTSAC 2 cut(s) 517, 661
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 517, 661
TspDTI ATGAA 5 cut(s) 72, 179, 294, 696, 727
TspGWI ACGGA 2 cut(s) 254, 651
TspRI CASTG 3 cut(s) 415, 487, 664
Tth111I GACNNNGTC 1 cut(s) 417
VpaK11BI GGWCC 1 cut(s) 419
XapI RAATTY 2 cut(s) 138, 290
XbaI TCTAGA 1 cut(s) 146
XmnI GAANNNNTTC 2 cut(s) 79, 290
XspI CTAG 2 cut(s) 147, 416
ZraI GACGTC 1 cut(s) 178
Zsp2I ATGCAT 1 cut(s) 253
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.