MD16G1283100.v1.1

psbP domain-containing protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0009272
Physical Location & Seq
Reverse (-)
39606755 .. 39608805
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1283100.v1.1.491

Sequence Viewer

Length: 774 bp
ATGGCGTGCGTTGCGTCCTGGTATTCTCTGCCTCCGGTGCGACCTCGCACATGCCACTCCAACGCCTTCTCGAACAACAGAGGCATCGCCGTACGCAGTAGAAAAAGTCATGCGCTCCTGCAATGTTGCAAAAACAAACAAGAAAAAACGGACACCAAAACAGAAACATGTTTTGGCATTGAATTCCAAGCTACGAGGAGACAAGTTGTGCTTGGGACGGCGTTTGTTGCATTTTCTTTTCCCCATCTCATTTCAAATGCATTGGCAGAGAATGGTAATGTGCCGGAGGGTTTTCGAGTTTACACTGACGATGTCAATAAGTTCAAGATATTGATTCCCCAAGACTGGCAAGTAGGCGCCGGAGAGCCAACTGGGTTTAAATCAGTCACTGCTTTCTACCCAGAACAAGAGGGTAATAGCTCAATTAGCGTTGTGATCACGGGGCTTGGTCCGGATTTTACAAAGATGGAATCCTTTGGTAAAGTTGAAGCGTTTGCTGAGACTCTGGTTAGTGGATTGGATAGGAGCTGGCAAAGGCCAGCAGGAGTGGCAGCAAAACTTATAGATTGCAAAGCATCGAAAGGCCTTTACTTCATCGAGTATTCACTACAAAAACCCGGTGAAAGTTGCAAGCACCTGTTTTCAGCACTTGGAATGGCAACCAACGGTTGGTACAACAGACTGTATACAGTCACCGGACAGTATGTCGAGGAAGAATCAGATAAATACAGCGCCAATGTTAAGGAGGCTGTCAAATCCTTCAGGTTCATTTGA

Protein Analysis

258

Amino Acids

28.52

Weight (kDa)

8.76

Isoelectric Point (pI)

38.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 96 - 256 4.1e-29 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 356
AccB7I CCANNNNNTGG 1 cut(s) 669
AccI GTMKAC 1 cut(s) 686
AccIII TCCGGA 1 cut(s) 451
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 1 cut(s) 745
AcyI GRCGYC 1 cut(s) 357
AfaI GTAC 2 cut(s) 93, 674
AfiI CCNNNNNNNGG 2 cut(s) 345, 669
AflIII ACRYGT 1 cut(s) 167
AgsI TTSAA 4 cut(s) 182, 255, 325, 488
AhdI GACNNNNNGTC 1 cut(s) 704
AjnI CCWGG 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 156, 188
AluBI AGCT 3 cut(s) 191, 420, 528
AluI AGCT 3 cut(s) 191, 420, 528
Alw26I GTCTC 2 cut(s) 193, 494
AlwNI CAGNNNCTG 1 cut(s) 389
Aor13HI TCCGGA 1 cut(s) 451
AoxI GGCC 2 cut(s) 536, 583
ApeKI GCWGC 1 cut(s) 551
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 3 cut(s) 115, 359, 734
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 1 cut(s) 618
AsuHPI GGTGA 2 cut(s) 632, 685
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 1 cut(s) 356
BbvI GCAGC 1 cut(s) 563
BccI CCATC 2 cut(s) 252, 460
BceAI ACGGC 2 cut(s) 74, 234
BciT130I CCWGG 1 cut(s) 19
BclI TGATCA 1 cut(s) 435
BcnI CCSGG 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 193, 494
BfoI RGCGCY 2 cut(s) 360, 735
BisI GCNGC 1 cut(s) 552
BlsI GCNGC 1 cut(s) 553
Bme1390I CCNGG 2 cut(s) 19, 618
Bme18I GGWCC 1 cut(s) 449
BmeRI GACNNNNNGTC 1 cut(s) 704
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 1 cut(s) 358
BmrFI CCNGG 2 cut(s) 19, 618
BmrI ACTGGG 1 cut(s) 381
BmsI GCATC 2 cut(s) 93, 584
BmuI ACTGGG 1 cut(s) 381
BpuMI CCSGG 1 cut(s) 618
BsaHI GRCGYC 1 cut(s) 357
BsaWI WCCGGW 3 cut(s) 34, 451, 695
Bsc4I CCNNNNNNNGG 2 cut(s) 345, 669
Bse1I ACTGG 2 cut(s) 350, 376
Bse3DI GCAATG 1 cut(s) 128
BseAI TCCGGA 1 cut(s) 451
BseBI CCWGG 1 cut(s) 19
BseLI CCNNNNNNNGG 2 cut(s) 345, 669
BseMI GCAATG 1 cut(s) 128
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 2 cut(s) 350, 376
BseRI GAGGAG 1 cut(s) 211
BseXI GCAGC 1 cut(s) 563
BshFI GGCC 2 cut(s) 538, 585
BshNI GGYRCC 1 cut(s) 356
BsiSI CCGG 6 cut(s) 35, 284, 360, 452, 618, 696
BsiWI CGTACG 1 cut(s) 91
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 2 cut(s) 345, 669
BsmAI GTCTC 2 cut(s) 193, 494
BsmFI GGGAC 1 cut(s) 229
BsnI GGCC 2 cut(s) 538, 585
Bsp13I TCCGGA 1 cut(s) 451
Bsp143I GATC 1 cut(s) 435
BspANI GGCC 2 cut(s) 538, 585
BspCNI CTCAG 1 cut(s) 490
BspEI TCCGGA 1 cut(s) 451
BspLI GGNNCC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 128
BsrI ACTGG 2 cut(s) 350, 376
BssMI GATC 1 cut(s) 435
BssNAI GTATAC 1 cut(s) 687
BssNI GRCGYC 1 cut(s) 357
Bst1107I GTATAC 1 cut(s) 687
Bst2UI CCWGG 1 cut(s) 19
Bst4CI ACNGT 4 cut(s) 668, 684, 691, 702
BstACI GRCGYC 1 cut(s) 357
BstC8I GCNNGC 4 cut(s) 7, 530, 540, 632
BstDEI CTNAG 1 cut(s) 498
BstH2I RGCGCY 2 cut(s) 360, 735
BstHHI GCGC 3 cut(s) 115, 359, 734
BstKTI GATC 1 cut(s) 438
BstMAI GTCTC 2 cut(s) 193, 494
BstMBI GATC 1 cut(s) 435
BstMWI GCNNNNNNNGC 5 cut(s) 11, 37, 227, 426, 548
BstNI CCWGG 1 cut(s) 19
BstNSI RCATGY 2 cut(s) 54, 171
BstSCI CCNGG 2 cut(s) 17, 616
BstV1I GCAGC 1 cut(s) 563
BstZ17I GTATAC 1 cut(s) 687
BsuRI GGCC 2 cut(s) 538, 585
BtgZI GCGATG 1 cut(s) 70
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 303, 387
Cac8I GCNNGC 4 cut(s) 7, 530, 540, 632
CaiI CAGNNNCTG 1 cut(s) 389
CfoI GCGC 3 cut(s) 115, 359, 734
Cfr13I GGNCC 1 cut(s) 449
CseI GACGC 1 cut(s) 3
Csp6I GTAC 2 cut(s) 92, 673
CviAII CATG 3 cut(s) 51, 110, 168
CviJI RGCY 8 cut(s) 191, 367, 420, 445, 528, 538, 585, 749
CviKI_1 RGCY 8 cut(s) 191, 367, 420, 445, 528, 538, 585, 749
CviQI GTAC 2 cut(s) 92, 673
DdeI CTNAG 1 cut(s) 498
DinI GGCGCC 1 cut(s) 358
DpnI GATC 1 cut(s) 437
DpnII GATC 1 cut(s) 435
DraI TTTAAA 1 cut(s) 379
DriI GACNNNNNGTC 1 cut(s) 704
Eam1105I GACNNNNNGTC 1 cut(s) 704
Eco147I AGGCCT 1 cut(s) 585
Eco47I GGWCC 1 cut(s) 449
Eco57I CTGAAG 1 cut(s) 745
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 17
EcoT22I ATGCAT 1 cut(s) 262
EgeI GGCGCC 1 cut(s) 358
EheI GGCGCC 1 cut(s) 358
FaeI CATG 3 cut(s) 54, 113, 171
FaiI YATR 6 cut(s) 52, 111, 169, 563, 687, 705
FalI AAGNNNNNCTT 2 cut(s) 195, 227
FaqI GGGAC 1 cut(s) 229
FatI CATG 3 cut(s) 50, 109, 167
FbaI TGATCA 1 cut(s) 435
FblI GTMKAC 1 cut(s) 686
Fnu4HI GCNGC 1 cut(s) 552
Fsp4HI GCNGC 1 cut(s) 552
GlaI GCGC 3 cut(s) 114, 358, 733
GluI GCNGC 1 cut(s) 552
HaeII RGCGCY 2 cut(s) 360, 735
HaeIII GGCC 2 cut(s) 538, 585
HapII CCGG 6 cut(s) 35, 284, 360, 452, 618, 696
HgaI GACGC 1 cut(s) 3
HhaI GCGC 3 cut(s) 115, 359, 734
Hin1I GRCGYC 1 cut(s) 357
Hin1II CATG 3 cut(s) 54, 113, 171
Hin6I GCGC 3 cut(s) 113, 357, 732
HinP1I GCGC 3 cut(s) 113, 357, 732
HinfI GANTC 4 cut(s) 334, 470, 502, 716
HpaII CCGG 6 cut(s) 35, 284, 360, 452, 618, 696
HphI GGTGA 2 cut(s) 632, 685
Hpy166II GTNNAC 2 cut(s) 301, 687
Hpy188I TCNGA 1 cut(s) 721
Hpy188III TCNNGA 3 cut(s) 70, 325, 452
Hpy8I GTNNAC 2 cut(s) 301, 687
HpyAV CCTTC 2 cut(s) 76, 769
HpyCH4III ACNGT 4 cut(s) 668, 684, 691, 702
HpyCH4V TGCA 6 cut(s) 121, 129, 230, 260, 570, 630
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 37, 227, 426, 548
HpyF3I CTNAG 1 cut(s) 498
Hsp92I GRCGYC 1 cut(s) 357
Hsp92II CATG 3 cut(s) 54, 113, 171
HspAI GCGC 3 cut(s) 113, 357, 732
KasI GGCGCC 1 cut(s) 356
Kpn2I TCCGGA 1 cut(s) 451
Ksp22I TGATCA 1 cut(s) 435
Kzo9I GATC 1 cut(s) 435
LmnI GCTCC 2 cut(s) 120, 525
Lsp1109I GCAGC 1 cut(s) 563
LweI GCATC 2 cut(s) 93, 584
MaeIII GTNAC 2 cut(s) 385, 691
MalI GATC 1 cut(s) 437
MboI GATC 1 cut(s) 435
MboII GAAGA 1 cut(s) 725
MluCI AATT 2 cut(s) 182, 423
Mly113I GGCGCC 1 cut(s) 357
MlyI GAGTC 1 cut(s) 496
MmeI TCCRAC 1 cut(s) 84
MnlI CCTC 8 cut(s) 42, 54, 74, 189, 280, 403, 703, 739
Mph1103I ATGCAT 1 cut(s) 262
MroI TCCGGA 1 cut(s) 451
MseI TTAA 2 cut(s) 378, 741
MspI CCGG 6 cut(s) 35, 284, 360, 452, 618, 696
MspR9I CCNGG 2 cut(s) 19, 618
MvaI CCWGG 1 cut(s) 19
MwoI GCNNNNNNNGC 5 cut(s) 11, 37, 227, 426, 548
NarI GGCGCC 1 cut(s) 357
NciI CCSGG 1 cut(s) 618
NdeII GATC 1 cut(s) 435
NlaIII CATG 3 cut(s) 54, 113, 171
NlaIV GGNNCC 1 cut(s) 358
NmuCI GTSAC 2 cut(s) 385, 691
NsiI ATGCAT 1 cut(s) 262
NspI RCATGY 2 cut(s) 54, 171
PceI AGGCCT 1 cut(s) 585
PciI ACATGT 1 cut(s) 167
PfeI GAWTC 3 cut(s) 334, 470, 716
Pfl23II CGTACG 1 cut(s) 91
PflFI GACNNNGTC 1 cut(s) 311
PflMI CCANNNNNTGG 1 cut(s) 669
PkrI GCNGC 1 cut(s) 553
PleI GAGTC 1 cut(s) 496
PluTI GGCGCC 1 cut(s) 360
PpsI GAGTC 1 cut(s) 496
PscI ACATGT 1 cut(s) 167
Psp6I CCWGG 1 cut(s) 17
PspGI CCWGG 1 cut(s) 17
PspLI CGTACG 1 cut(s) 91
PspN4I GGNNCC 1 cut(s) 358
PspPI GGNCC 1 cut(s) 449
PstNI CAGNNNCTG 1 cut(s) 389
PsyI GACNNNGTC 1 cut(s) 311
RsaI GTAC 2 cut(s) 93, 674
RsaNI GTAC 2 cut(s) 92, 673
SaqAI TTAA 2 cut(s) 378, 741
SatI GCNGC 1 cut(s) 552
Sau3AI GATC 1 cut(s) 435
Sau96I GGNCC 1 cut(s) 449
SchI GAGTC 1 cut(s) 496
ScrFI CCNGG 2 cut(s) 19, 618
SetI ASST 6 cut(s) 46, 193, 422, 530, 639, 767
SfaNI GCATC 2 cut(s) 93, 584
SfoI GGCGCC 1 cut(s) 358
SinI GGWCC 1 cut(s) 449
Sse9I AATT 2 cut(s) 182, 423
SseBI AGGCCT 1 cut(s) 585
SspDI GGCGCC 1 cut(s) 356
StuI AGGCCT 1 cut(s) 585
StyD4I CCNGG 2 cut(s) 17, 616
TaaI ACNGT 4 cut(s) 668, 684, 691, 702
TaqI TCGA 5 cut(s) 71, 295, 578, 597, 708
TasI AATT 2 cut(s) 182, 423
TfiI GAWTC 3 cut(s) 334, 470, 716
Tru1I TTAA 2 cut(s) 378, 741
Tru9I TTAA 2 cut(s) 378, 741
TscAI CASTG 2 cut(s) 310, 394
TseFI GTSAC 2 cut(s) 385, 691
TseI GCWGC 1 cut(s) 551
Tsp45I GTSAC 2 cut(s) 385, 691
TspDTI ATGAA 2 cut(s) 583, 757
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 2 cut(s) 310, 394
Tth111I GACNNNGTC 1 cut(s) 311
Van91I CCANNNNNTGG 1 cut(s) 669
VpaK11BI GGWCC 1 cut(s) 449
XapI RAATTY 1 cut(s) 182
XceI RCATGY 2 cut(s) 54, 171
XmiI GTMKAC 1 cut(s) 686
Zsp2I ATGCAT 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.