Rroxscaffold_3G00243010

psbP domain-containing protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009272
Physical Location & Seq
Reverse (-)
35539785 .. 35541696
1912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00243010.1

Sequence Viewer

Length: 759 bp
ATGGCTTCCGTTGCGTCTTGGCATCCTCTGTTCCTTCGACCTCGCACAAGCCATTTCACCGCAACCTCGAACAACCCAGGTGAAGCTCTCAGCTCAGCTCCACAGTCCACACCCAGTTCCTACCTAATCTCCATCTTTTATGTTACCATTGACAGTTTTGGGATGCAATGCCAAGGCAAGAGAAGACAAGTTTTGCTTGGGACGACTTTTGCTGCATTTTCTTTTCTGGAAATTTATTCAAACATTGCATTGGCCGAGAATGTTTATGTGGCAGAGGGTTTTCGTGTTTTCACCGATGATGTAAACAAGTTCCAGATATCAATTCCCTTAGACTGGCAAGTAGGCGCAGGAGAGCCAAGTGGGTTCAAGTCAGTTACTGCTTTCTACCCGGAAGAGGGCTTTAGCTCAATTAGTGTCGTAATCACGGGGCTTGGTCCGGATTTTACAAAGATGGAATCCTTTGGCAAGGTTGAAGCCTTTGCTGAGACTCTGGTCAGCGGACTAGATAGGAGCTGGCAAAGGCCAGCAGGAGTTTCAGCGAAACTCATAGATTGCAAATCATCTAAAGGGCTTTACTACATTGAGTATTCACTGCAAAATCCTGGTGAAAGTATGAAGCACCTCTATTCAGCTCTTGGGATGGCAACCAACGGCTGGTACAACAGATTATATACCGTCACCGGCCAGTTTGCAGAGGAAGAATCGGATAAGTACAGTTCCAAAATTGAGAAGGCTGTAAAATCCTTTAAGTTCATATGA

Protein Analysis

252

Amino Acids

27.86

Weight (kDa)

5.97

Isoelectric Point (pI)

39.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PsbP PF01789 92 - 251 1.2e-28 PsbP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 654
AccIII TCCGGA 1 cut(s) 436
AciI CCGC 2 cut(s) 60, 498
AcoI YGGCCR 2 cut(s) 252, 682
AcsI RAATTY 1 cut(s) 231
AfaI GTAC 2 cut(s) 659, 713
AfiI CCNNNNNNNGG 4 cut(s) 333, 394, 395, 654
AgsI TTSAA 3 cut(s) 240, 367, 473
AjnI CCWGG 2 cut(s) 76, 601
AluBI AGCT 6 cut(s) 86, 93, 98, 405, 513, 632
AluI AGCT 6 cut(s) 86, 93, 98, 405, 513, 632
Alw26I GTCTC 1 cut(s) 479
AlwNI CAGNNNCTG 1 cut(s) 377
Aor13HI TCCGGA 1 cut(s) 436
AoxI GGCC 3 cut(s) 252, 521, 682
ApeKI GCWGC 1 cut(s) 212
ApoI RAATTY 1 cut(s) 231
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 1 cut(s) 434
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 5 cut(s) 49, 92, 283, 617, 670
AvaII GGWCC 1 cut(s) 434
BbsI GAAGAC 1 cut(s) 190
BbvI GCAGC 1 cut(s) 199
BccI CCATC 3 cut(s) 140, 445, 634
BceAI ACGGC 1 cut(s) 667
BciT130I CCWGG 2 cut(s) 78, 603
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 1 cut(s) 479
BfaI CTAG 1 cut(s) 503
BisI GCNGC 1 cut(s) 213
BlpI GCTNAGC 1 cut(s) 94
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 78, 389, 603
Bme18I GGWCC 1 cut(s) 434
BmgT120I GGNCC 1 cut(s) 434
BmrFI CCNGG 3 cut(s) 78, 389, 603
BmrI ACTGGG 1 cut(s) 108
BmsI GCATC 2 cut(s) 31, 153
BmuI ACTGGG 1 cut(s) 108
BoxI GACNNNNGTC 1 cut(s) 491
BpiI GAAGAC 1 cut(s) 190
Bpu1102I GCTNAGC 1 cut(s) 94
BpuMI CCSGG 1 cut(s) 389
BsaJI CCNNGG 2 cut(s) 76, 172
BsaWI WCCGGW 1 cut(s) 436
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 4 cut(s) 333, 394, 395, 654
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 3 cut(s) 114, 338, 685
Bse3DI GCAATG 2 cut(s) 173, 243
BseAI TCCGGA 1 cut(s) 436
BseBI CCWGG 2 cut(s) 78, 603
BseDI CCNNGG 2 cut(s) 76, 172
BseGI GGATG 3 cut(s) 22, 168, 645
BseLI CCNNNNNNNGG 4 cut(s) 333, 394, 395, 654
BseMI GCAATG 2 cut(s) 173, 243
BseMII CTCAG 3 cut(s) 103, 108, 474
BseNI ACTGG 3 cut(s) 114, 338, 685
BseXI GCAGC 1 cut(s) 199
BshFI GGCC 3 cut(s) 254, 523, 684
BsiSI CCGG 3 cut(s) 389, 437, 681
BslFI GGGAC 1 cut(s) 214
BslI CCNNNNNNNGG 4 cut(s) 333, 394, 395, 654
BsmAI GTCTC 1 cut(s) 479
BsmFI GGGAC 1 cut(s) 214
BsnI GGCC 3 cut(s) 254, 523, 684
Bsp13I TCCGGA 1 cut(s) 436
Bsp1720I GCTNAGC 1 cut(s) 94
BspACI CCGC 2 cut(s) 60, 498
BspANI GGCC 3 cut(s) 254, 523, 684
BspCNI CTCAG 3 cut(s) 102, 107, 475
BspEI TCCGGA 1 cut(s) 436
BsrDI GCAATG 2 cut(s) 173, 243
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 3 cut(s) 114, 338, 685
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 2 cut(s) 76, 172
BssT1I CCWWGG 1 cut(s) 172
Bst2UI CCWGG 2 cut(s) 78, 603
Bst4CI ACNGT 4 cut(s) 105, 155, 676, 716
Bst6I CTCTTC 1 cut(s) 387
BstC8I GCNNGC 2 cut(s) 515, 525
BstDEI CTNAG 4 cut(s) 89, 94, 328, 483
BstF5I GGATG 3 cut(s) 22, 168, 645
BstHHI GCGC 1 cut(s) 347
BstMAI GTCTC 1 cut(s) 479
BstMWI GCNNNNNNNGC 1 cut(s) 11
BstNI CCWGG 2 cut(s) 78, 603
BstPAI GACNNNNGTC 1 cut(s) 491
BstSCI CCNGG 3 cut(s) 76, 387, 601
BstV1I GCAGC 1 cut(s) 199
BstV2I GAAGAC 1 cut(s) 190
BsuRI GGCC 3 cut(s) 254, 523, 684
BtsCI GGATG 3 cut(s) 22, 168, 645
BtsI GCAGTG 1 cut(s) 590
BtsIMutI CAGTG 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 515, 525
CaiI CAGNNNCTG 1 cut(s) 377
CfoI GCGC 1 cut(s) 347
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 1 cut(s) 434
CseI GACGC 1 cut(s) 3
Csp6I GTAC 2 cut(s) 658, 712
CviQI GTAC 2 cut(s) 658, 712
DdeI CTNAG 4 cut(s) 89, 94, 328, 483
EaeI YGGCCR 2 cut(s) 252, 682
Eam1104I CTCTTC 1 cut(s) 387
EarI CTCTTC 1 cut(s) 387
Eco130I CCWWGG 1 cut(s) 172
Eco32I GATATC 1 cut(s) 318
Eco47I GGWCC 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 76, 601
EcoRV GATATC 1 cut(s) 318
EcoT14I CCWWGG 1 cut(s) 172
ErhI CCWWGG 1 cut(s) 172
FaiI YATR 8 cut(s) 141, 267, 548, 614, 670, 672, 755, 757
FalI AAGNNNNNCTT 2 cut(s) 180, 212
FaqI GGGAC 1 cut(s) 214
FauNDI CATATG 1 cut(s) 755
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 3 cut(s) 9, 175, 652
Fsp4HI GCNGC 1 cut(s) 213
FspBI CTAG 1 cut(s) 503
GlaI GCGC 1 cut(s) 346
GluI GCNGC 1 cut(s) 213
HaeIII GGCC 3 cut(s) 254, 523, 684
HapII CCGG 3 cut(s) 389, 437, 681
HgaI GACGC 1 cut(s) 3
HhaI GCGC 1 cut(s) 347
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HinfI GANTC 3 cut(s) 455, 487, 701
HpaII CCGG 3 cut(s) 389, 437, 681
HphI GGTGA 5 cut(s) 49, 92, 283, 617, 670
Hpy166II GTNNAC 2 cut(s) 108, 304
Hpy188I TCNGA 1 cut(s) 706
Hpy188III TCNNGA 3 cut(s) 227, 313, 437
Hpy8I GTNNAC 2 cut(s) 108, 304
HpyAV CCTTC 2 cut(s) 44, 724
HpyCH4III ACNGT 4 cut(s) 105, 155, 676, 716
HpyCH4V TGCA 6 cut(s) 166, 215, 248, 555, 595, 692
HpyF10VI GCNNNNNNNGC 1 cut(s) 11
HpyF3I CTNAG 4 cut(s) 89, 94, 328, 483
HspAI GCGC 1 cut(s) 345
Kpn2I TCCGGA 1 cut(s) 436
LmnI GCTCC 2 cut(s) 103, 510
Lsp1109I GCAGC 1 cut(s) 199
LweI GCATC 2 cut(s) 31, 153
MaeI CTAG 1 cut(s) 503
MaeIII GTNAC 3 cut(s) 142, 373, 676
MboII GAAGA 3 cut(s) 195, 404, 710
MluCI AATT 4 cut(s) 231, 321, 408, 723
MlyI GAGTC 1 cut(s) 481
MnlI CCTC 7 cut(s) 36, 51, 76, 268, 388, 632, 688
MroI TCCGGA 1 cut(s) 436
MseI TTAA 1 cut(s) 747
MspA1I CMGCKG 1 cut(s) 498
MspI CCGG 3 cut(s) 389, 437, 681
MspR9I CCNGG 3 cut(s) 78, 389, 603
MvaI CCWGG 2 cut(s) 78, 603
MwoI GCNNNNNNNGC 1 cut(s) 11
NciI CCSGG 1 cut(s) 389
NdeI CATATG 1 cut(s) 755
NmeAIII GCCGAG 1 cut(s) 280
NmuCI GTSAC 1 cut(s) 676
PfeI GAWTC 2 cut(s) 455, 701
PflMI CCANNNNNTGG 1 cut(s) 654
PkrI GCNGC 1 cut(s) 214
PleI GAGTC 1 cut(s) 481
PpsI GAGTC 1 cut(s) 481
PshAI GACNNNNGTC 1 cut(s) 491
Psp6I CCWGG 2 cut(s) 76, 601
PspGI CCWGG 2 cut(s) 76, 601
PspPI GGNCC 1 cut(s) 434
PstNI CAGNNNCTG 1 cut(s) 377
RsaI GTAC 2 cut(s) 659, 713
RsaNI GTAC 2 cut(s) 658, 712
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 1 cut(s) 213
Sau96I GGNCC 1 cut(s) 434
SchI GAGTC 1 cut(s) 481
ScrFI CCNGG 3 cut(s) 78, 389, 603
SfaNI GCATC 2 cut(s) 31, 153
SinI GGWCC 1 cut(s) 434
Sse9I AATT 4 cut(s) 231, 321, 408, 723
SsiI CCGC 2 cut(s) 60, 498
SspMI CTAG 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 76, 387, 601
StyI CCWWGG 1 cut(s) 172
TaaI ACNGT 4 cut(s) 105, 155, 676, 716
TaqI TCGA 2 cut(s) 37, 68
TasI AATT 4 cut(s) 231, 321, 408, 723
TatI WGTACW 1 cut(s) 711
TfiI GAWTC 2 cut(s) 455, 701
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 1 cut(s) 597
TseFI GTSAC 1 cut(s) 676
TseI GCWGC 1 cut(s) 212
Tsp45I GTSAC 1 cut(s) 676
TspDTI ATGAA 2 cut(s) 629, 742
TspRI CASTG 1 cut(s) 597
Van91I CCANNNNNTGG 1 cut(s) 654
VpaK11BI GGWCC 1 cut(s) 434
XapI RAATTY 1 cut(s) 231
XspI CTAG 1 cut(s) 503
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.