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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G76740 |
| fragaria_vesca | FvH4_2g13350 FvH4_2g13350 |
| malus_domestica | MD05G1029500.v1.1 |
| prunus_persica | Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 Prupe.8G035200_v2.0.a1 |
| pyrus_communis | pycom05g02080 |
| rosa_chinensis | RchiOBHm_Chr6g0274831 |
| rosa_laevigata | RLG00000013518 |
| rosa_multiflora | Rmu_sc0008611.1_g000008 |
| rosa_roxburghii | Rroxscaffold_7G00193900 |
| rosa_rugosa | Rorug06G0085100 |
| rosa_samantha | Rh6AG200700 Rh6BG204100 Rh6CG204400 Rh6DG195600 |
| rosa_wichuraiana | Rw6G017450 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 3925 |
| AccB1I | GGYRCC | 1 cut(s) | 3925 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 748 |
| AccI | GTMKAC | 4 cut(s) | 642, 772, 1478, 2187 |
| AccIII | TCCGGA | 2 cut(s) | 2149, 2608 |
| AclI | AACGTT | 1 cut(s) | 1183 |
| AclWI | GGATC | 5 cut(s) | 36, 361, 708, 1258, 4024 |
| AcoI | YGGCCR | 2 cut(s) | 1054, 1900 |
| AcsI | RAATTY | 5 cut(s) | 4, 67, 233, 1717, 3960 |
| AcuI | CTGAAG | 6 cut(s) | 144, 1833, 2925, 3495, 4076, 4380 |
| AcvI | CACGTG | 1 cut(s) | 3522 |
| AfaI | GTAC | 5 cut(s) | 1387, 1821, 1854, 2071, 3927 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 748, 2433, 3935 |
| AflII | CTTAAG | 1 cut(s) | 1982 |
| AflIII | ACRYGT | 1 cut(s) | 3322 |
| AgeI | ACCGGT | 2 cut(s) | 390, 449 |
| AhlI | ACTAGT | 1 cut(s) | 2726 |
| AjnI | CCWGG | 4 cut(s) | 1420, 2190, 3123, 3574 |
| Alw21I | GWGCWC | 2 cut(s) | 2808, 4098 |
| AlwI | GGATC | 5 cut(s) | 36, 361, 708, 1258, 4024 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 3059 |
| Ama87I | CYCGRG | 1 cut(s) | 3257 |
| Aor13HI | TCCGGA | 2 cut(s) | 2149, 2608 |
| AoxI | GGCC | 4 cut(s) | 1054, 1900, 1954, 2571 |
| ApeKI | GCWGC | 9 cut(s) | 734, 1625, 2483, 3194, 3344, 3515, 3632, 4229, 4316 |
| ApoI | RAATTY | 5 cut(s) | 4, 67, 233, 1717, 3960 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 2940, 2972 |
| AsiGI | ACCGGT | 2 cut(s) | 390, 449 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 3901, 3991 |
| Asp718I | GGTACC | 1 cut(s) | 3925 |
| AspA2I | CCTAGG | 1 cut(s) | 905 |
| AspLEI | GCGC | 2 cut(s) | 2399, 4054 |
| AspS9I | GGNCC | 4 cut(s) | 418, 1954, 2364, 4235 |
| AsuC2I | CCSGG | 1 cut(s) | 2837 |
| AsuHPI | GGTGA | 8 cut(s) | 767, 1082, 1214, 1238, 1593, 1659, 1833, 3446 |
| AsuII | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| AsuNHI | GCTAGC | 1 cut(s) | 2393 |
| AvaI | CYCGRG | 1 cut(s) | 3257 |
| AvaII | GGWCC | 3 cut(s) | 418, 2364, 4235 |
| AvrII | CCTAGG | 1 cut(s) | 905 |
| AxyI | CCTNAGG | 1 cut(s) | 4162 |
| BaeGI | GKGCMC | 1 cut(s) | 3357 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1956, 1989 |
| BalI | TGGCCA | 1 cut(s) | 1056 |
| BanI | GGYRCC | 1 cut(s) | 3925 |
| BanII | GRGCYC | 4 cut(s) | 1992, 2808, 3467, 4098 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 430, 462 |
| BauI | CACGAG | 1 cut(s) | 4336 |
| BbrPI | CACGTG | 1 cut(s) | 3522 |
| BbsI | GAAGAC | 5 cut(s) | 471, 605, 2352, 3180, 4371 |
| Bbv12I | GWGCWC | 2 cut(s) | 2808, 4098 |
| BbvCI | CCTCAGC | 1 cut(s) | 1926 |
| BbvI | GCAGC | 9 cut(s) | 721, 1612, 2495, 3181, 3331, 3527, 3644, 4241, 4303 |
| BceAI | ACGGC | 1 cut(s) | 4227 |
| BciT130I | CCWGG | 4 cut(s) | 1422, 2192, 3125, 3576 |
| BciVI | GTATCC | 1 cut(s) | 133 |
| BclI | TGATCA | 3 cut(s) | 541, 3040, 4332 |
| BcnI | CCSGG | 1 cut(s) | 2837 |
| BcuI | ACTAGT | 1 cut(s) | 2726 |
| BfmI | CTRYAG | 2 cut(s) | 1998, 4317 |
| BfoI | RGCGCY | 1 cut(s) | 2400 |
| BfrI | CTTAAG | 1 cut(s) | 1982 |
| BfuI | GTATCC | 1 cut(s) | 133 |
| BglII | AGATCT | 2 cut(s) | 830, 2963 |
| BisI | GCNGC | 9 cut(s) | 735, 1626, 2484, 3195, 3345, 3516, 3633, 4230, 4317 |
| BlnI | CCTAGG | 1 cut(s) | 905 |
| BlpI | GCTNAGC | 1 cut(s) | 1881 |
| BlsI | GCNGC | 9 cut(s) | 736, 1627, 2485, 3196, 3346, 3517, 3634, 4231, 4318 |
| Bme1390I | CCNGG | 5 cut(s) | 1422, 2192, 2837, 3125, 3576 |
| Bme18I | GGWCC | 3 cut(s) | 418, 2364, 4235 |
| BmeT110I | CYCGRG | 1 cut(s) | 3257 |
| BmgT120I | GGNCC | 4 cut(s) | 418, 1954, 2364, 4235 |
| BmiI | GGNNCC | 9 cut(s) | 420, 882, 1002, 1314, 1531, 1989, 2653, 3927, 3988 |
| BmrFI | CCNGG | 5 cut(s) | 1422, 2192, 2837, 3125, 3576 |
| BmsI | GCATC | 9 cut(s) | 1148, 1306, 2145, 2187, 3692, 3775, 4039, 4206, 4425 |
| BmtI | GCTAGC | 1 cut(s) | 2397 |
| BpiI | GAAGAC | 5 cut(s) | 471, 605, 2352, 3180, 4371 |
| BplI | GAGNNNNNCTC | 6 cut(s) | 1979, 2011, 3926, 3958, 4558, 4590 |
| BpmI | CTGGAG | 9 cut(s) | 95, 1233, 2213, 2246, 2638, 3146, 3534, 3621, 4206 |
| Bpu10I | CCTNAGC | 3 cut(s) | 308, 789, 1926 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1881 |
| Bpu14I | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| BpuEI | CTTGAG | 7 cut(s) | 197, 233, 1043, 1692, 1775, 2297, 3652 |
| BpuMI | CCSGG | 1 cut(s) | 2837 |
| BsaAI | YACGTR | 1 cut(s) | 3522 |
| BsaI | GGTCTC | 2 cut(s) | 249, 949 |
| BsaJI | CCNNGG | 7 cut(s) | 422, 905, 1421, 1944, 3574, 3933, 3996 |
| BsaWI | WCCGGW | 5 cut(s) | 390, 449, 1669, 2149, 2608 |
| BsaXI | ACNNNNNCTCC | 8 cut(s) | 1206, 1236, 1752, 1782, 3532, 3562, 4560, 4590 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 748, 2433, 3935 |
| Bse118I | RCCGGY | 3 cut(s) | 390, 449, 1898 |
| Bse1I | ACTGG | 1 cut(s) | 951 |
| Bse21I | CCTNAGG | 1 cut(s) | 4162 |
| Bse3DI | GCAATG | 1 cut(s) | 2718 |
| BseAI | TCCGGA | 2 cut(s) | 2149, 2608 |
| BseBI | CCWGG | 4 cut(s) | 1422, 2192, 3125, 3576 |
| BseDI | CCNNGG | 7 cut(s) | 422, 905, 1421, 1944, 3574, 3933, 3996 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 748, 2433, 3935 |
| BseMI | GCAATG | 1 cut(s) | 2718 |
| BseNI | ACTGG | 1 cut(s) | 951 |
| BseRI | GAGGAG | 5 cut(s) | 1522, 1802, 1964, 3881, 4150 |
| BseSI | GKGCMC | 1 cut(s) | 3357 |
| BseXI | GCAGC | 9 cut(s) | 721, 1612, 2495, 3181, 3331, 3527, 3644, 4241, 4303 |
| BseYI | CCCAGC | 2 cut(s) | 61, 2558 |
| BsgI | GTGCAG | 1 cut(s) | 3756 |
| Bsh1285I | CGRYCG | 1 cut(s) | 431 |
| BshFI | GGCC | 4 cut(s) | 1056, 1902, 1956, 2573 |
| BshNI | GGYRCC | 1 cut(s) | 3925 |
| BshTI | ACCGGT | 2 cut(s) | 390, 449 |
| BsiEI | CGRYCG | 1 cut(s) | 431 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2808, 4098 |
| BsiHKCI | CYCGRG | 1 cut(s) | 3257 |
| BsiSI | CCGG | 8 cut(s) | 391, 450, 1670, 1899, 2150, 2574, 2609, 2837 |
| BslFI | GGGAC | 5 cut(s) | 404, 1877, 2575, 3200, 3811 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 748, 2433, 3935 |
| BsmBI | CGTCTC | 1 cut(s) | 568 |
| BsmFI | GGGAC | 5 cut(s) | 404, 1877, 2575, 3200, 3811 |
| BsmI | GAATGC | 4 cut(s) | 1568, 2117, 3820, 4513 |
| BsnI | GGCC | 4 cut(s) | 1056, 1902, 1956, 2573 |
| Bso31I | GGTCTC | 2 cut(s) | 249, 949 |
| BsoBI | CYCGRG | 1 cut(s) | 3257 |
| Bsp119I | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| Bsp1286I | GDGCHC | 5 cut(s) | 1992, 2808, 3357, 3467, 4098 |
| Bsp13I | TCCGGA | 2 cut(s) | 2149, 2608 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1881 |
| Bsp19I | CCATGG | 1 cut(s) | 3996 |
| BspANI | GGCC | 4 cut(s) | 1056, 1902, 1956, 2573 |
| BspEI | TCCGGA | 2 cut(s) | 2149, 2608 |
| BspHI | TCATGA | 1 cut(s) | 2704 |
| BspLI | GGNNCC | 9 cut(s) | 420, 882, 1002, 1314, 1531, 1989, 2653, 3927, 3988 |
| BspMAI | CTGCAG | 1 cut(s) | 4321 |
| BspOI | GCTAGC | 1 cut(s) | 2397 |
| BspPI | GGATC | 5 cut(s) | 36, 361, 708, 1258, 4024 |
| BspT104I | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| BspT107I | GGYRCC | 1 cut(s) | 3925 |
| BspTI | CTTAAG | 1 cut(s) | 1982 |
| BspTNI | GGTCTC | 2 cut(s) | 249, 949 |
| BsrDI | GCAATG | 1 cut(s) | 2718 |
| BsrFI | RCCGGY | 3 cut(s) | 390, 449, 1898 |
| BsrI | ACTGG | 1 cut(s) | 951 |
| BssAI | RCCGGY | 3 cut(s) | 390, 449, 1898 |
| BssECI | CCNNGG | 7 cut(s) | 422, 905, 1421, 1944, 3574, 3933, 3996 |
| BssNAI | GTATAC | 1 cut(s) | 1479 |
| BssSI | CACGAG | 1 cut(s) | 4336 |
| BssT1I | CCWWGG | 4 cut(s) | 422, 905, 1944, 3996 |
| Bst1107I | GTATAC | 1 cut(s) | 1479 |
| Bst2BI | CACGAG | 1 cut(s) | 4336 |
| Bst2UI | CCWGG | 4 cut(s) | 1422, 2192, 3125, 3576 |
| BstAFI | CTTAAG | 1 cut(s) | 1982 |
| BstBAI | YACGTR | 1 cut(s) | 3522 |
| BstBI | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| BstC8I | GCNNGC | 2 cut(s) | 2395, 2481 |
| BstDSI | CCRYGG | 1 cut(s) | 3996 |
| BstEII | GGTNACC | 1 cut(s) | 386 |
| BstH2I | RGCGCY | 1 cut(s) | 2400 |
| BstHHI | GCGC | 2 cut(s) | 2399, 4054 |
| BstMCI | CGRYCG | 1 cut(s) | 431 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 319, 734, 1508, 1987, 3929, 4211, 4489 |
| BstNI | CCWGG | 4 cut(s) | 1422, 2192, 3125, 3576 |
| BstNSI | RCATGY | 4 cut(s) | 1506, 3326, 3735, 3833 |
| BstPI | GGTNACC | 1 cut(s) | 386 |
| BstSCI | CCNGG | 5 cut(s) | 1420, 2190, 2835, 3123, 3574 |
| BstSFI | CTRYAG | 2 cut(s) | 1998, 4317 |
| BstSLI | GKGCMC | 1 cut(s) | 3357 |
| BstV1I | GCAGC | 9 cut(s) | 721, 1612, 2495, 3181, 3331, 3527, 3644, 4241, 4303 |
| BstV2I | GAAGAC | 5 cut(s) | 471, 605, 2352, 3180, 4371 |
| BstX2I | RGATCY | 6 cut(s) | 28, 353, 830, 1263, 2963, 4016 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2644 |
| BstYI | RGATCY | 6 cut(s) | 28, 353, 830, 1263, 2963, 4016 |
| BstZ17I | GTATAC | 1 cut(s) | 1479 |
| Bsu36I | CCTNAGG | 1 cut(s) | 4162 |
| BsuI | GTATCC | 1 cut(s) | 133 |
| BsuRI | GGCC | 4 cut(s) | 1056, 1902, 1956, 2573 |
| BtgI | CCRYGG | 1 cut(s) | 3996 |
| BtsI | GCAGTG | 1 cut(s) | 1134 |
| Cac8I | GCNNGC | 2 cut(s) | 2395, 2481 |
| CaiI | CAGNNNCTG | 1 cut(s) | 3059 |
| CciI | TCATGA | 1 cut(s) | 2704 |
| CfoI | GCGC | 2 cut(s) | 2399, 4054 |
| Cfr10I | RCCGGY | 3 cut(s) | 390, 449, 1898 |
| Cfr13I | GGNCC | 4 cut(s) | 418, 1954, 2364, 4235 |
| CseI | GACGC | 3 cut(s) | 585, 1517, 2485 |
| Csp6I | GTAC | 5 cut(s) | 1386, 1820, 1853, 2070, 3926 |
| CspAI | ACCGGT | 2 cut(s) | 390, 449 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 3457, 3492 |
| CviQI | GTAC | 5 cut(s) | 1386, 1820, 1853, 2070, 3926 |
| EaeI | YGGCCR | 2 cut(s) | 1054, 1900 |
| EciI | GGCGGA | 1 cut(s) | 4229 |
| Ecl136II | GAGCTC | 2 cut(s) | 2806, 4096 |
| Eco130I | CCWWGG | 4 cut(s) | 422, 905, 1944, 3996 |
| Eco24I | GRGCYC | 4 cut(s) | 1992, 2808, 3467, 4098 |
| Eco31I | GGTCTC | 2 cut(s) | 249, 949 |
| Eco32I | GATATC | 1 cut(s) | 594 |
| Eco47I | GGWCC | 3 cut(s) | 418, 2364, 4235 |
| Eco53kI | GAGCTC | 2 cut(s) | 2806, 4096 |
| Eco57I | CTGAAG | 6 cut(s) | 144, 1833, 2925, 3495, 4076, 4380 |
| Eco72I | CACGTG | 1 cut(s) | 3522 |
| Eco81I | CCTNAGG | 1 cut(s) | 4162 |
| Eco88I | CYCGRG | 1 cut(s) | 3257 |
| Eco91I | GGTNACC | 1 cut(s) | 386 |
| EcoICRI | GAGCTC | 2 cut(s) | 2806, 4096 |
| EcoO109I | RGGNCCY | 2 cut(s) | 418, 1954 |
| EcoO65I | GGTNACC | 1 cut(s) | 386 |
| EcoRI | GAATTC | 2 cut(s) | 233, 1717 |
| EcoRII | CCWGG | 4 cut(s) | 1420, 2190, 3123, 3574 |
| EcoRV | GATATC | 1 cut(s) | 594 |
| EcoT14I | CCWWGG | 4 cut(s) | 422, 905, 1944, 3996 |
| EcoT38I | GRGCYC | 4 cut(s) | 1992, 2808, 3467, 4098 |
| ErhI | CCWWGG | 4 cut(s) | 422, 905, 1944, 3996 |
| Esp3I | CGTCTC | 1 cut(s) | 568 |
| FalI | AAGNNNNNCTT | 6 cut(s) | 651, 683, 1737, 1769, 3493, 3525 |
| FaqI | GGGAC | 5 cut(s) | 404, 1877, 2575, 3200, 3811 |
| FauI | CCCGC | 3 cut(s) | 1395, 2743, 3709 |
| FbaI | TGATCA | 3 cut(s) | 541, 3040, 4332 |
| FblI | GTMKAC | 4 cut(s) | 642, 772, 1478, 2187 |
| Fnu4HI | GCNGC | 9 cut(s) | 735, 1626, 2484, 3195, 3345, 3516, 3633, 4230, 4317 |
| FriOI | GRGCYC | 4 cut(s) | 1992, 2808, 3467, 4098 |
| Fsp4HI | GCNGC | 9 cut(s) | 735, 1626, 2484, 3195, 3345, 3516, 3633, 4230, 4317 |
| GlaI | GCGC | 2 cut(s) | 2398, 4053 |
| GluI | GCNGC | 9 cut(s) | 735, 1626, 2484, 3195, 3345, 3516, 3633, 4230, 4317 |
| GsaI | CCCAGC | 2 cut(s) | 65, 2562 |
| GsuI | CTGGAG | 9 cut(s) | 95, 1233, 2213, 2246, 2638, 3146, 3534, 3621, 4206 |
| HaeII | RGCGCY | 1 cut(s) | 2400 |
| HaeIII | GGCC | 4 cut(s) | 1056, 1902, 1956, 2573 |
| HapII | CCGG | 8 cut(s) | 391, 450, 1670, 1899, 2150, 2574, 2609, 2837 |
| HgaI | GACGC | 3 cut(s) | 585, 1517, 2485 |
| HhaI | GCGC | 2 cut(s) | 2399, 4054 |
| Hin6I | GCGC | 2 cut(s) | 2397, 4052 |
| HinP1I | GCGC | 2 cut(s) | 2397, 4052 |
| HincII | GTYRAC | 3 cut(s) | 2289, 3235, 3664 |
| HindII | GTYRAC | 3 cut(s) | 2289, 3235, 3664 |
| HindIII | AAGCTT | 1 cut(s) | 1112 |
| HpaII | CCGG | 8 cut(s) | 391, 450, 1670, 1899, 2150, 2574, 2609, 2837 |
| HphI | GGTGA | 8 cut(s) | 767, 1082, 1214, 1238, 1593, 1659, 1833, 3446 |
| HpyCH4IV | ACGT | 7 cut(s) | 919, 1183, 1454, 3521, 3627, 4424, 4542 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 319, 734, 1508, 1987, 3929, 4211, 4489 |
| HpySE526I | ACGT | 7 cut(s) | 919, 1183, 1454, 3521, 3627, 4424, 4542 |
| HspAI | GCGC | 2 cut(s) | 2397, 4052 |
| Kpn2I | TCCGGA | 2 cut(s) | 2149, 2608 |
| KpnI | GGTACC | 1 cut(s) | 3929 |
| Ksp22I | TGATCA | 3 cut(s) | 541, 3040, 4332 |
| Lsp1109I | GCAGC | 9 cut(s) | 721, 1612, 2495, 3181, 3331, 3527, 3644, 4241, 4303 |
| LweI | GCATC | 9 cut(s) | 1148, 1306, 2145, 2187, 3692, 3775, 4039, 4206, 4425 |
| MaeII | ACGT | 7 cut(s) | 919, 1183, 1454, 3521, 3627, 4424, 4542 |
| MfeI | CAATTG | 3 cut(s) | 3951, 4107, 4239 |
| MflI | RGATCY | 6 cut(s) | 28, 353, 830, 1263, 2963, 4016 |
| MhlI | GDGCHC | 5 cut(s) | 1992, 2808, 3357, 3467, 4098 |
| MlsI | TGGCCA | 1 cut(s) | 1056 |
| MluNI | TGGCCA | 1 cut(s) | 1056 |
| MmeI | TCCRAC | 3 cut(s) | 1332, 2012, 2247 |
| Mox20I | TGGCCA | 1 cut(s) | 1056 |
| MroI | TCCGGA | 2 cut(s) | 2149, 2608 |
| MroXI | GAANNNNTTC | 2 cut(s) | 3901, 3991 |
| MscI | TGGCCA | 1 cut(s) | 1056 |
| MslI | CAYNNNNRTG | 3 cut(s) | 83, 2505, 4305 |
| Msp20I | TGGCCA | 1 cut(s) | 1056 |
| MspA1I | CMGCKG | 4 cut(s) | 262, 589, 2486, 4232 |
| MspCI | CTTAAG | 1 cut(s) | 1982 |
| MspI | CCGG | 8 cut(s) | 391, 450, 1670, 1899, 2150, 2574, 2609, 2837 |
| MspR9I | CCNGG | 5 cut(s) | 1422, 2192, 2837, 3125, 3576 |
| MunI | CAATTG | 3 cut(s) | 3951, 4107, 4239 |
| Mva1269I | GAATGC | 4 cut(s) | 1568, 2117, 3820, 4513 |
| MvaI | CCWGG | 4 cut(s) | 1422, 2192, 3125, 3576 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 319, 734, 1508, 1987, 3929, 4211, 4489 |
| NciI | CCSGG | 1 cut(s) | 2837 |
| NcoI | CCATGG | 1 cut(s) | 3996 |
| NheI | GCTAGC | 1 cut(s) | 2393 |
| NlaIV | GGNNCC | 9 cut(s) | 420, 882, 1002, 1314, 1531, 1989, 2653, 3927, 3988 |
| NmeAIII | GCCGAG | 1 cut(s) | 3958 |
| NmuCI | GTSAC | 3 cut(s) | 1505, 3318, 3623 |
| NspI | RCATGY | 4 cut(s) | 1506, 3326, 3735, 3833 |
| NspV | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| PacI | TTAATTAA | 1 cut(s) | 3570 |
| PagI | TCATGA | 1 cut(s) | 2704 |
| PciI | ACATGT | 1 cut(s) | 3322 |
| PctI | GAATGC | 4 cut(s) | 1568, 2117, 3820, 4513 |
| PdmI | GAANNNNTTC | 2 cut(s) | 3901, 3991 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 748 |
| PinAI | ACCGGT | 2 cut(s) | 390, 449 |
| PkrI | GCNGC | 9 cut(s) | 736, 1627, 2485, 3196, 3346, 3517, 3634, 4231, 4318 |
| PmaCI | CACGTG | 1 cut(s) | 3522 |
| PmlI | CACGTG | 1 cut(s) | 3522 |
| Ppu21I | YACGTR | 1 cut(s) | 3522 |
| PpuMI | RGGWCCY | 1 cut(s) | 418 |
| PscI | ACATGT | 1 cut(s) | 3322 |
| Psp124BI | GAGCTC | 2 cut(s) | 2808, 4098 |
| Psp1406I | AACGTT | 1 cut(s) | 1183 |
| Psp5II | RGGWCCY | 1 cut(s) | 418 |
| Psp6I | CCWGG | 4 cut(s) | 1420, 2190, 3123, 3574 |
| PspCI | CACGTG | 1 cut(s) | 3522 |
| PspEI | GGTNACC | 1 cut(s) | 386 |
| PspFI | CCCAGC | 2 cut(s) | 61, 2558 |
| PspGI | CCWGG | 4 cut(s) | 1420, 2190, 3123, 3574 |
| PspN4I | GGNNCC | 9 cut(s) | 420, 882, 1002, 1314, 1531, 1989, 2653, 3927, 3988 |
| PspPI | GGNCC | 4 cut(s) | 418, 1954, 2364, 4235 |
| PspPPI | RGGWCCY | 1 cut(s) | 418 |
| PstI | CTGCAG | 1 cut(s) | 4321 |
| PstNI | CAGNNNCTG | 1 cut(s) | 3059 |
| PsuI | RGATCY | 6 cut(s) | 28, 353, 830, 1263, 2963, 4016 |
| PvuII | CAGCTG | 3 cut(s) | 589, 2486, 4232 |
| RsaI | GTAC | 5 cut(s) | 1387, 1821, 1854, 2071, 3927 |
| RsaNI | GTAC | 5 cut(s) | 1386, 1820, 1853, 2070, 3926 |
| RseI | CAYNNNNRTG | 3 cut(s) | 83, 2505, 4305 |
| SacI | GAGCTC | 2 cut(s) | 2808, 4098 |
| SatI | GCNGC | 9 cut(s) | 735, 1626, 2484, 3195, 3345, 3516, 3633, 4230, 4317 |
| Sau96I | GGNCC | 4 cut(s) | 418, 1954, 2364, 4235 |
| ScrFI | CCNGG | 5 cut(s) | 1422, 2192, 2837, 3125, 3576 |
| SduI | GDGCHC | 5 cut(s) | 1992, 2808, 3357, 3467, 4098 |
| SfaNI | GCATC | 9 cut(s) | 1148, 1306, 2145, 2187, 3692, 3775, 4039, 4206, 4425 |
| SfcI | CTRYAG | 2 cut(s) | 1998, 4317 |
| SfuI | TTCGAA | 3 cut(s) | 914, 1127, 4137 |
| SinI | GGWCC | 3 cut(s) | 418, 2364, 4235 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 83, 2505, 4305 |
| SmlI | CTYRAG | 8 cut(s) | 176, 248, 1022, 1707, 1754, 1982, 2312, 3667 |
| SmoI | CTYRAG | 8 cut(s) | 176, 248, 1022, 1707, 1754, 1982, 2312, 3667 |
| SpeI | ACTAGT | 1 cut(s) | 2726 |
| SspI | AATATT | 1 cut(s) | 3901 |
| SstI | GAGCTC | 2 cut(s) | 2808, 4098 |
| StyD4I | CCNGG | 5 cut(s) | 1420, 2190, 2835, 3123, 3574 |
| StyI | CCWWGG | 4 cut(s) | 422, 905, 1944, 3996 |
| TaiI | ACGT | 7 cut(s) | 922, 1186, 1457, 3524, 3630, 4427, 4545 |
| TaqI | TCGA | 8 cut(s) | 414, 431, 596, 914, 1127, 1292, 3169, 4137 |
| TaqII | GACCGA | 1 cut(s) | 231 |
| TatI | WGTACW | 2 cut(s) | 1819, 1852 |
| TseFI | GTSAC | 3 cut(s) | 1505, 3318, 3623 |
| TseI | GCWGC | 9 cut(s) | 734, 1625, 2483, 3194, 3344, 3515, 3632, 4229, 4316 |
| Tsp45I | GTSAC | 3 cut(s) | 1505, 3318, 3623 |
| TspGWI | ACGGA | 5 cut(s) | 152, 494, 1256, 2253, 3394 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 748 |
| Vha464I | CTTAAG | 1 cut(s) | 1982 |
| VpaK11BI | GGWCC | 3 cut(s) | 418, 2364, 4235 |
| XapI | RAATTY | 5 cut(s) | 4, 67, 233, 1717, 3960 |
| XbaI | TCTAGA | 1 cut(s) | 2731 |
| XceI | RCATGY | 4 cut(s) | 1506, 3326, 3735, 3833 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 2641 |
| XmaJI | CCTAGG | 1 cut(s) | 905 |
| XmiI | GTMKAC | 4 cut(s) | 642, 772, 1478, 2187 |
| XmnI | GAANNNNTTC | 2 cut(s) | 3901, 3991 |