Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003872

Physical Location & Seq

Genomic Coordinates Reverse (-)

28922415 .. 28925468

Gene Structure 3054 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G76970.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1341 bp

ATGGCAAATGATGCGGCTGCATGTGCAGAAAGGGCTACAAATGATATGCTGATTGGTCCAGATTGGGCTATTAACATTGAATTGTGTGATCTTATCAACATGGATCCTAGTCAAGCAAAAGAAGCTGTGAAGGTGCTCAAGAAGCGGTTAGGAAGTAAAAACTCTAAAGTCCAGATTCTTGCACTTTATGCATTGGAAACTTTAAGTAAGAATTGTGGCGAAAACGTGTACCAGCTTATCATTGACCGTGGTCTATTGAATGACATGGTCAAAATAGTGAAGAAAAAGCCGGAACTGAATGTGAGGGAAAAGATACTAACTTTGTTAGACACATGGCAAGAAGCCTTTGGTGGACGTGGAGGTCGATACCCACAATACTACAATGCCTATAACGATCTCAGGTCTGCTGGAATTGAGTTTCCGCCTCGAACGGAAAGCAGTCTATCGTTCTTTACTCCACCGCAGACTCAGCCAGATGAAGATGCTGCTATTCAGGCTTCGTTGCAAGGAGATGATGCTTCTAGCCTCAGCCTGGAAGAGATTCAAAGCGCTGAGGGATCAGTTGATGTTTTGATGGACATGCTTGGAGCACATGATCCCGGGAATCCCGAGAGTTTAAAAGAAGAAGTTATAGTAGACTTGGTTGAGCAATGTCGTACTTATCAAAGACGTGTAATGACTCTTGTGAACACTACAACAGATGAGGAACTTCTGTGTCAGGGATTGGCATTAAACGATAATTTGCAGCATGTTCTTCAGCGTCACGATGATATCGCAAATGTCGGCTCTGTTCCTTCAAATGGAAGGAATACTAGAGCTCCTCCTCCAGTTCAGATTGTTGACATCAATCATGATGATGAAGATGACGAATCAGATGATGAGTTTGCTCGGCTTGCTCACAGGTCTTCAACACCTACAAGGAGACCAGTACATGGTAGTGATTCGGGTATGGTGGATATCCTTAGTGGGGACGTCTATAAACCTCAAGGCAACTCCTCCTCACAGGGAGTAAAGAAGCCTCCTCCTCCTCCTCCGCATACTTCTTCTTCCTCTTCTTCTCCTGTTTTCGATGATGCAAGTCCACAACAAAGCAAATCCTCTGAGGTAATTAGGAATCTTCCACCTCCGCCTTCAAGGCACAACCAGAGGCAACAGTTCTTTGAGCATCACCACTCGAGTAGCGGCTCAGATTCCTCTTACGAGGGACAGACCCGGAACCTTTCTTTGACAAGTAGTGAACCGCAGAAAGAAGAGAAACCAGAGGATTTGCTTTTCAAAGACCTGGTTGAGTTCGCCAAAACCAGGTCCTCCAAAGCCAACAATAATAATAGGTCGCTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007088 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030276 GO:0033043 GO:0044424 GO:0044464 GO:0045786 GO:0045839 GO:0045930 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GAT VHS

Protein Analysis

446

Amino Acids

49.25

Weight (kDa)

4.97

Isoelectric Point (pI)

55.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
VHS	PF00790	10 - 119	8.5e-30	VHS domain
GAT	PF03127	186 - 258	1.1e-18	GAT domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003872)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21380 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970
fragaria_vesca	FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400
malus_domestica	MD05G1008800.v1.1 MD10G1007100.v1.1 MD10G1007700.v1.1
prunus_persica	Prupe.8G010900_v2.0.a1 Prupe.8G010900_v2.0.a1
pyrus_communis	pycom05g00450 pycom10g00480
rosa_chinensis	RchiOBHm_Chr6g0278141
rosa_laevigata	RLG00000013238
rosa_multiflora	Rmu_co8265659.1_g000001 Rmu_sc0000791.1_g000057 Rmu_sc0000857.1_g000058
rosa_roxburghii	Rroxscaffold_7G00190500
rosa_rugosa	Rorug06G0114900
rosa_samantha	Rh6AG224900 Rh6BG229000 Rh6CG231500 Rh6DG222300
rosa_wichuraiana	Rw6G019660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	360
AatII	GACGTC	1 cut(s)	975
AccB7I	CCANNNNNTGG	1 cut(s)	932
AccI	GTMKAC	1 cut(s)	636
AciI	CCGC	8 cut(s)	14, 145, 422, 461, 1034, 1127, 1182, 1241
AclWI	GGATC	4 cut(s)	98, 111, 565, 590
AcuI	CTGAAG	1 cut(s)	740
AcyI	GRCGYC	1 cut(s)	972
AfaI	GTAC	3 cut(s)	230, 658, 930
AfeI	AGCGCT	1 cut(s)	550
AfiI	CCNNNNNNNGG	5 cut(s)	532, 800, 932, 967, 1302
AflIII	ACRYGT	2 cut(s)	225, 670
AgsI	TTSAA	7 cut(s)	80, 259, 545, 798, 909, 1134, 1276
AjiI	CACGTC	2 cut(s)	356, 671
AjnI	CCWGG	3 cut(s)	531, 1281, 1301
AluBI	AGCT	3 cut(s)	125, 235, 818
AluI	AGCT	3 cut(s)	125, 235, 818
Alw21I	GWGCWC	3 cut(s)	138, 592, 820
Alw26I	GTCTC	1 cut(s)	916
AlwI	GGATC	4 cut(s)	98, 111, 565, 590
Ama87I	CYCGRG	3 cut(s)	599, 608, 1174
Aor51HI	AGCGCT	1 cut(s)	550
ApeKI	GCWGC	3 cut(s)	17, 485, 745
Asp700I	GAANNNNTTC	1 cut(s)	540
AspLEI	GCGC	1 cut(s)	551
AspS9I	GGNCC	2 cut(s)	56, 1305
AsuC2I	CCSGG	3 cut(s)	600, 601, 1213
AsuHPI	GGTGA	1 cut(s)	1160
AvaI	CYCGRG	3 cut(s)	599, 608, 1174
AvaII	GGWCC	2 cut(s)	56, 1305
BamHI	GGATCC	1 cut(s)	103
BanII	GRGCYC	1 cut(s)	820
BbsI	GAAGAC	1 cut(s)	897
Bbv12I	GWGCWC	3 cut(s)	138, 592, 820
BbvCI	CCTCAGC	2 cut(s)	527, 552
BbvI	GCAGC	3 cut(s)	4, 472, 757
BccI	CCATC	1 cut(s)	568
BciT130I	CCWGG	3 cut(s)	533, 1283, 1303
BcnI	CCSGG	3 cut(s)	600, 601, 1213
BcoDI	GTCTC	1 cut(s)	916
BfaI	CTAG	3 cut(s)	108, 522, 813
BfoI	RGCGCY	1 cut(s)	552
BglI	GCCNNNNNGGC	1 cut(s)	1135
BisI	GCNGC	5 cut(s)	15, 18, 486, 746, 1183
BlsI	GCNGC	5 cut(s)	16, 19, 487, 747, 1184
Bme1390I	CCNGG	6 cut(s)	533, 600, 601, 1213, 1283, 1303
Bme18I	GGWCC	2 cut(s)	56, 1305
BmeT110I	CYCGRG	3 cut(s)	599, 608, 1174
BmgBI	CACGTC	2 cut(s)	356, 671
BmgT120I	GGNCC	2 cut(s)	56, 1305
BmiI	GGNNCC	2 cut(s)	105, 1217
BmrFI	CCNGG	6 cut(s)	533, 600, 601, 1213, 1283, 1303
BmsI	GCATC	4 cut(s)	472, 505, 1063, 1174
BoxI	GACNNNNGTC	1 cut(s)	249
BpiI	GAAGAC	1 cut(s)	897
BpmI	CTGGAG	1 cut(s)	810
Bpu10I	CCTNAGC	2 cut(s)	527, 552
BpuEI	CTTGAG	2 cut(s)	122, 969
BpuMI	CCSGG	3 cut(s)	600, 601, 1213
BsaHI	GRCGYC	1 cut(s)	972
BsaI	GGTCTC	1 cut(s)	916
BsaJI	CCNNGG	2 cut(s)	247, 599
BsaXI	ACNNNNNCTCC	2 cut(s)	802, 832
Bsc4I	CCNNNNNNNGG	5 cut(s)	532, 800, 932, 967, 1302
Bse1I	ACTGG	2 cut(s)	827, 926
Bse3DI	GCAATG	1 cut(s)	656
BseBI	CCWGG	3 cut(s)	533, 1283, 1303
BseDI	CCNNGG	2 cut(s)	247, 599
BseLI	CCNNNNNNNGG	5 cut(s)	532, 800, 932, 967, 1302
BseMI	GCAATG	1 cut(s)	656
BseMII	CTCAG	6 cut(s)	412, 482, 541, 543, 1092, 1200
BseNI	ACTGG	2 cut(s)	827, 926
BseRI	GAGGAG	8 cut(s)	810, 813, 985, 988, 1011, 1014, 1017, 1020
BseXI	GCAGC	3 cut(s)	4, 472, 757
BsgI	GTGCAG	1 cut(s)	45
BsiHKAI	GWGCWC	3 cut(s)	138, 592, 820
BsiHKCI	CYCGRG	3 cut(s)	599, 608, 1174
BsiSI	CCGG	3 cut(s)	290, 600, 1213
BslFI	GGGAC	2 cut(s)	983, 1218
BslI	CCNNNNNNNGG	5 cut(s)	532, 800, 932, 967, 1302
BsmAI	GTCTC	1 cut(s)	916
BsmFI	GGGAC	2 cut(s)	983, 1218
Bso31I	GGTCTC	1 cut(s)	916
BsoBI	CYCGRG	3 cut(s)	599, 608, 1174
Bsp1286I	GDGCHC	3 cut(s)	138, 592, 820
Bsp143I	GATC	5 cut(s)	88, 103, 394, 557, 595
BspACI	CCGC	8 cut(s)	14, 145, 422, 461, 1034, 1127, 1182, 1241
BspCNI	CTCAG	6 cut(s)	411, 481, 540, 544, 1093, 1199
BspHI	TCATGA	1 cut(s)	850
BspLI	GGNNCC	2 cut(s)	105, 1217
BspPI	GGATC	4 cut(s)	98, 111, 565, 590
BspTNI	GGTCTC	1 cut(s)	916
BsrDI	GCAATG	1 cut(s)	656
BsrI	ACTGG	2 cut(s)	827, 926
BssECI	CCNNGG	2 cut(s)	247, 599
BssMI	GATC	5 cut(s)	88, 103, 394, 557, 595
BssNI	GRCGYC	1 cut(s)	972
Bst2UI	CCWGG	3 cut(s)	533, 1283, 1303
Bst4CI	ACNGT	2 cut(s)	248, 1155
Bst6I	CTCTTC	3 cut(s)	531, 1057, 1245
BstACI	GRCGYC	1 cut(s)	972
BstAPI	GCANNNNNTGC	2 cut(s)	11, 188
BstC8I	GCNNGC	1 cut(s)	894
BstDEI	CTNAG	7 cut(s)	398, 468, 527, 552, 962, 1101, 1186
BstDSI	CCRYGG	1 cut(s)	247
BstH2I	RGCGCY	1 cut(s)	552
BstHHI	GCGC	1 cut(s)	551
BstKTI	GATC	5 cut(s)	91, 106, 397, 560, 598
BstMAI	GTCTC	1 cut(s)	916
BstMBI	GATC	5 cut(s)	88, 103, 394, 557, 595
BstNI	CCWGG	3 cut(s)	533, 1283, 1303
BstNSI	RCATGY	3 cut(s)	24, 583, 752
BstPAI	GACNNNNGTC	1 cut(s)	249
BstSCI	CCNGG	6 cut(s)	531, 598, 599, 1211, 1281, 1301
BstV1I	GCAGC	3 cut(s)	4, 472, 757
BstV2I	GAAGAC	1 cut(s)	897
BstX2I	RGATCY	1 cut(s)	103
BstYI	RGATCY	1 cut(s)	103
BtgI	CCRYGG	1 cut(s)	247
BtrI	CACGTC	2 cut(s)	356, 671
Cac8I	GCNNGC	1 cut(s)	894
CciI	TCATGA	1 cut(s)	850
CfoI	GCGC	1 cut(s)	551
Cfr13I	GGNCC	2 cut(s)	56, 1305
Cfr9I	CCCGGG	1 cut(s)	599
CseI	GACGC	1 cut(s)	749
CsiI	ACCWGGT	2 cut(s)	1281, 1301
Csp6I	GTAC	3 cut(s)	229, 657, 929
CviAII	CATG	9 cut(s)	21, 100, 265, 333, 580, 593, 749, 851, 932
CviQI	GTAC	3 cut(s)	229, 657, 929
DdeI	CTNAG	7 cut(s)	398, 468, 527, 552, 962, 1101, 1186
DpnI	GATC	5 cut(s)	90, 105, 396, 559, 597
DpnII	GATC	5 cut(s)	88, 103, 394, 557, 595
DraI	TTTAAA	1 cut(s)	618
DrdI	GACNNNNNNGTC	1 cut(s)	360
DseDI	GACNNNNNNGTC	1 cut(s)	360
Eam1104I	CTCTTC	3 cut(s)	531, 1057, 1245
EarI	CTCTTC	3 cut(s)	531, 1057, 1245
EciI	GGCGGA	2 cut(s)	411, 1116
Ecl136II	GAGCTC	1 cut(s)	818
Eco24I	GRGCYC	1 cut(s)	820
Eco31I	GGTCTC	1 cut(s)	916
Eco32I	GATATC	2 cut(s)	772, 958
Eco47I	GGWCC	2 cut(s)	56, 1305
Eco47III	AGCGCT	1 cut(s)	550
Eco53kI	GAGCTC	1 cut(s)	818
Eco57I	CTGAAG	1 cut(s)	740
Eco88I	CYCGRG	3 cut(s)	599, 608, 1174
EcoICRI	GAGCTC	1 cut(s)	818
EcoO109I	RGGNCCY	1 cut(s)	1305
EcoRII	CCWGG	3 cut(s)	531, 1281, 1301
EcoRV	GATATC	2 cut(s)	772, 958
EcoT22I	ATGCAT	1 cut(s)	193
EcoT38I	GRGCYC	1 cut(s)	820
FaeI	CATG	9 cut(s)	24, 103, 268, 336, 583, 596, 752, 854, 935
FaqI	GGGAC	2 cut(s)	983, 1218
FatI	CATG	9 cut(s)	20, 99, 264, 332, 579, 592, 748, 850, 931
FblI	GTMKAC	1 cut(s)	636
Fnu4HI	GCNGC	5 cut(s)	15, 18, 486, 746, 1183
FriOI	GRGCYC	1 cut(s)	820
Fsp4HI	GCNGC	5 cut(s)	15, 18, 486, 746, 1183
FspBI	CTAG	3 cut(s)	108, 522, 813
GlaI	GCGC	1 cut(s)	550
GluI	GCNGC	5 cut(s)	15, 18, 486, 746, 1183
GsuI	CTGGAG	1 cut(s)	810
HaeII	RGCGCY	1 cut(s)	552
HapII	CCGG	3 cut(s)	290, 600, 1213
HgaI	GACGC	1 cut(s)	749
HhaI	GCGC	1 cut(s)	551
Hin1I	GRCGYC	1 cut(s)	972
Hin1II	CATG	9 cut(s)	24, 103, 268, 336, 583, 596, 752, 854, 935
Hin6I	GCGC	1 cut(s)	549
HinP1I	GCGC	1 cut(s)	549
HincII	GTYRAC	1 cut(s)	841
HindII	GTYRAC	1 cut(s)	841
HinfI	GANTC	9 cut(s)	175, 466, 541, 604, 679, 869, 941, 1114, 1190
HpaII	CCGG	3 cut(s)	290, 600, 1213
HphI	GGTGA	1 cut(s)	1160
Hpy166II	GTNNAC	7 cut(s)	229, 353, 637, 688, 841, 1082, 1238
Hpy188I	TCNGA	5 cut(s)	834, 874, 1102, 1189, 1340
Hpy188III	TCNNGA	6 cut(s)	59, 139, 172, 608, 764, 851
Hpy8I	GTNNAC	7 cut(s)	229, 353, 637, 688, 841, 1082, 1238
HpyAV	CCTTC	4 cut(s)	124, 798, 804, 1140
HpyCH4III	ACNGT	2 cut(s)	248, 1155
HpyCH4IV	ACGT	4 cut(s)	225, 355, 670, 972
HpyCH4V	TGCA	7 cut(s)	20, 26, 182, 191, 505, 745, 1076
HpyF3I	CTNAG	7 cut(s)	398, 468, 527, 552, 962, 1101, 1186
HpySE526I	ACGT	4 cut(s)	225, 355, 670, 972
Hsp92I	GRCGYC	1 cut(s)	972
Hsp92II	CATG	9 cut(s)	24, 103, 268, 336, 583, 596, 752, 854, 935
HspAI	GCGC	1 cut(s)	549
Kzo9I	GATC	5 cut(s)	88, 103, 394, 557, 595
LmnI	GCTCC	2 cut(s)	587, 823
Lsp1109I	GCAGC	3 cut(s)	4, 472, 757
LweI	GCATC	4 cut(s)	472, 505, 1063, 1174
MabI	ACCWGGT	2 cut(s)	1281, 1301
MaeI	CTAG	3 cut(s)	108, 522, 813
MaeII	ACGT	4 cut(s)	225, 355, 670, 972
MaeIII	GTNAC	1 cut(s)	761
MalI	GATC	5 cut(s)	90, 105, 396, 559, 597
MboI	GATC	5 cut(s)	88, 103, 394, 557, 595
MflI	RGATCY	1 cut(s)	103
MhlI	GDGCHC	3 cut(s)	138, 592, 820
MluCI	AATT	5 cut(s)	80, 211, 411, 739, 1107
MlyI	GAGTC	2 cut(s)	460, 673
Mph1103I	ATGCAT	1 cut(s)	193
MroXI	GAANNNNTTC	1 cut(s)	540
MseI	TTAA	4 cut(s)	72, 203, 617, 731
MslI	CAYNNNNRTG	2 cut(s)	855, 936
MspI	CCGG	3 cut(s)	290, 600, 1213
MspR9I	CCNGG	6 cut(s)	533, 600, 601, 1213, 1283, 1303
MvaI	CCWGG	3 cut(s)	533, 1283, 1303
NciI	CCSGG	3 cut(s)	600, 601, 1213
NdeII	GATC	5 cut(s)	88, 103, 394, 557, 595
NlaIII	CATG	9 cut(s)	24, 103, 268, 336, 583, 596, 752, 854, 935
NlaIV	GGNNCC	2 cut(s)	105, 1217
NmeAIII	GCCGAG	1 cut(s)	868
NmuCI	GTSAC	1 cut(s)	761
NsiI	ATGCAT	1 cut(s)	193
NspI	RCATGY	3 cut(s)	24, 583, 752
PaeR7I	CTCGAG	1 cut(s)	1174
PagI	TCATGA	1 cut(s)	850
PcsI	WCGNNNNNNNCGW	1 cut(s)	361
PdmI	GAANNNNTTC	1 cut(s)	540
PfeI	GAWTC	7 cut(s)	175, 541, 604, 869, 941, 1114, 1190
PflFI	GACNNNGTC	1 cut(s)	266
PflMI	CCANNNNNTGG	1 cut(s)	932
PkrI	GCNGC	5 cut(s)	16, 19, 487, 747, 1184
PleI	GAGTC	2 cut(s)	460, 673
PpsI	GAGTC	2 cut(s)	460, 673
PpuMI	RGGWCCY	1 cut(s)	1305
PshAI	GACNNNNGTC	1 cut(s)	249
Psp124BI	GAGCTC	1 cut(s)	820
Psp5II	RGGWCCY	1 cut(s)	1305
Psp6I	CCWGG	3 cut(s)	531, 1281, 1301
PspGI	CCWGG	3 cut(s)	531, 1281, 1301
PspN4I	GGNNCC	2 cut(s)	105, 1217
PspPI	GGNCC	2 cut(s)	56, 1305
PspPPI	RGGWCCY	1 cut(s)	1305
PspXI	VCTCGAGB	1 cut(s)	1174
PsuI	RGATCY	1 cut(s)	103
PsyI	GACNNNGTC	1 cut(s)	266
RsaI	GTAC	3 cut(s)	230, 658, 930
RsaNI	GTAC	3 cut(s)	229, 657, 929
RseI	CAYNNNNRTG	2 cut(s)	855, 936
SacI	GAGCTC	1 cut(s)	820
SaqAI	TTAA	4 cut(s)	72, 203, 617, 731
SatI	GCNGC	5 cut(s)	15, 18, 486, 746, 1183
Sau3AI	GATC	5 cut(s)	88, 103, 394, 557, 595
Sau96I	GGNCC	2 cut(s)	56, 1305
SchI	GAGTC	2 cut(s)	460, 673
ScrFI	CCNGG	6 cut(s)	533, 600, 601, 1213, 1283, 1303
SduI	GDGCHC	3 cut(s)	138, 592, 820
SexAI	ACCWGGT	2 cut(s)	1281, 1301
SfaNI	GCATC	4 cut(s)	472, 505, 1063, 1174
Sfr274I	CTCGAG	1 cut(s)	1174
SinI	GGWCC	2 cut(s)	56, 1305
SlaI	CTCGAG	1 cut(s)	1174
SmaI	CCCGGG	1 cut(s)	601
SmiMI	CAYNNNNRTG	2 cut(s)	855, 936
SmlI	CTYRAG	3 cut(s)	137, 984, 1174
SmoI	CTYRAG	3 cut(s)	137, 984, 1174
Sse9I	AATT	5 cut(s)	80, 211, 411, 739, 1107
SsiI	CCGC	8 cut(s)	14, 145, 422, 461, 1034, 1127, 1182, 1241
SspMI	CTAG	3 cut(s)	108, 522, 813
SstI	GAGCTC	1 cut(s)	820
StyD4I	CCNGG	6 cut(s)	531, 598, 599, 1211, 1281, 1301
TaaI	ACNGT	2 cut(s)	248, 1155
TaiI	ACGT	4 cut(s)	228, 358, 673, 975
TaqI	TCGA	4 cut(s)	364, 427, 1068, 1175
TasI	AATT	5 cut(s)	80, 211, 411, 739, 1107
TatI	WGTACW	1 cut(s)	928
TauI	GCSGC	2 cut(s)	17, 1185
TfiI	GAWTC	7 cut(s)	175, 541, 604, 869, 941, 1114, 1190
Tru1I	TTAA	4 cut(s)	72, 203, 617, 731
Tru9I	TTAA	4 cut(s)	72, 203, 617, 731
TseFI	GTSAC	1 cut(s)	761
TseI	GCWGC	3 cut(s)	17, 485, 745
Tsp45I	GTSAC	1 cut(s)	761
TspDTI	ATGAA	2 cut(s)	492, 873
TspGWI	ACGGA	1 cut(s)	446
TspMI	CCCGGG	1 cut(s)	599
Tth111I	GACNNNGTC	1 cut(s)	266
Van91I	CCANNNNNTGG	1 cut(s)	932
VpaK11BI	GGWCC	2 cut(s)	56, 1305
XceI	RCATGY	3 cut(s)	24, 583, 752
XhoI	CTCGAG	1 cut(s)	1174
XmaI	CCCGGG	1 cut(s)	599
XmiI	GTMKAC	1 cut(s)	636
XmnI	GAANNNNTTC	1 cut(s)	540
XspI	CTAG	3 cut(s)	108, 522, 813
ZraI	GACGTC	1 cut(s)	973
Zsp2I	ATGCAT	1 cut(s)	193

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.