Rroxscaffold_7G00190500

TOM1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0003872
Physical Location & Seq
Reverse (-)
30634421 .. 30641066
6646 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00190500.1

Sequence Viewer

Length: 996 bp
ATGGAAATGCTTAATGCCTTGGATGCTAATAATCCCGAGGGTCTGAAGGAAGAAGTTATTGTTGACCTTGTTGATCAGTGCAGTTCGTACCAAAAGCGTGTCATGCTTCTTGTGAATAACACTGCAGATGAGTCCCTTCTGTTTCAGGGATTGGCACTGAACGATGATCTGCAGAAGGTACTTAGTCGGCATGATGACATGGTGAAAGGAACTACAACAGGAGTAAGAGGACCGGAATCTTCTGTTGTGCCACTTGTGAATGTGAACCATGAGGATGATGAGTCGGAGGATGAATTTGCCCAACTAGCTCACAGGTCATCGAGAGATAATTCACAGGGACAGGGCCAGAGATCAGCCAACTCCAATACTGAACCAGTGCGAGCCAGCCCATTTCTTCCTCCTCCACCCTCTTCAAAAAGGCCAATAACCCTTGGTCCTGGTCTAATTGATTATCTCAGTGGTGAAGCCTACAAATCTGGAGGATCCCCTGCAACTTCAGAATCCACGTCCTTTGCAGTTCCCGCTCATTCGAGCCCCAATTCCAGTTCACAGTTGACTCCATTGTCTTCCTCAGCCCCTTCATCCCATTCCACAAACACGACTCCATCACCTTTGTTCACTGGAAAGCCTATTTACGATGAACCTGCTCCAAGAAGCAAATCCATTGATGGGCTGCCTCCAGCTCCTTGGGATGCACAGGCTCCTGGAGGTTCTGTGTCTCCTGGAGGTTCTCAATCTCCTGTCAGCCTTCCACCTCCACCATCAAGGTATAATCAGAGACAACAGTTTTTTGAGCAGCAAGCAGGTGGTGCTCCTCAAACAAGTAGTGGGTCTGGTTCGTCTTATGACAGTTTAGTGGGACAGACTCAGAACCTCTCTCTTAATTCATCTACCCCCACGAAACAAGTAAAACAGGAAGATGCCCTTTTCAAAGACCTCGTCGATTTTGCGAAAGCCAAGTCATCTTCGTCTGCAAAACCCAATAGATCATACTGA

Protein Analysis

331

Amino Acids

35.03

Weight (kDa)

5.01

Isoelectric Point (pI)

65.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GAT PF03127 2 - 70 6.3e-17 GAT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 794
AasI GACNNNNNNGTC 1 cut(s) 562
Acc36I ACCTGC 2 cut(s) 652, 794
AccBSI CCGCTC 1 cut(s) 524
AciI CCGC 1 cut(s) 522
AclWI GGATC 2 cut(s) 477, 490
AcsI RAATTY 1 cut(s) 293
AcuI CTGAAG 2 cut(s) 65, 480
AfaI GTAC 2 cut(s) 89, 180
AfiI CCNNNNNNNGG 1 cut(s) 669
AgsI TTSAA 2 cut(s) 414, 931
AjiI CACGTC 1 cut(s) 507
AjnI CCWGG 3 cut(s) 436, 703, 721
AluBI AGCT 2 cut(s) 308, 683
AluI AGCT 2 cut(s) 308, 683
Alw21I GWGCWC 1 cut(s) 814
Alw26I GTCTC 2 cut(s) 723, 772
AlwI GGATC 2 cut(s) 477, 490
Ama87I CYCGRG 1 cut(s) 35
AoxI GGCC 2 cut(s) 343, 419
ApeKI GCWGC 2 cut(s) 673, 796
ApoI RAATTY 1 cut(s) 293
ArsI GACNNNNNNTTYG 4 cut(s) 924, 944, 956, 976
AspS9I GGNCC 3 cut(s) 230, 343, 434
AsuHPI GGTGA 3 cut(s) 214, 473, 600
AvaI CYCGRG 1 cut(s) 35
AvaII GGWCC 2 cut(s) 230, 434
BamHI GGATCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 536
BbsI GAAGAC 1 cut(s) 558
Bbv12I GWGCWC 1 cut(s) 814
BbvCI CCTCAGC 1 cut(s) 571
BbvI GCAGC 2 cut(s) 660, 808
BccI CCATC 3 cut(s) 613, 662, 769
BcgI CGANNNNNNTGC 2 cut(s) 626, 660
BciT130I CCWGG 3 cut(s) 438, 705, 723
BclI TGATCA 1 cut(s) 73
BcoDI GTCTC 2 cut(s) 723, 772
BfaI CTAG 1 cut(s) 305
BfmI CTRYAG 2 cut(s) 123, 170
BfuAI ACCTGC 2 cut(s) 652, 794
BisI GCNGC 2 cut(s) 674, 797
BlsI GCNGC 2 cut(s) 675, 798
Bme1390I CCNGG 3 cut(s) 438, 705, 723
Bme18I GGWCC 2 cut(s) 230, 434
BmeT110I CYCGRG 1 cut(s) 35
BmgBI CACGTC 1 cut(s) 507
BmgT120I GGNCC 3 cut(s) 230, 343, 434
BmiI GGNNCC 2 cut(s) 484, 702
BmrFI CCNGG 3 cut(s) 438, 705, 723
BmsI GCATC 3 cut(s) 13, 682, 910
BpiI GAAGAC 1 cut(s) 558
BpmI CTGGAG 4 cut(s) 498, 663, 726, 744
Bpu10I CCTNAGC 1 cut(s) 571
BsaJI CCNNGG 4 cut(s) 18, 36, 430, 686
BsaWI WCCGGW 1 cut(s) 232
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 1 cut(s) 669
Bse1I ACTGG 3 cut(s) 374, 543, 625
BseBI CCWGG 3 cut(s) 438, 705, 723
BseDI CCNNGG 4 cut(s) 18, 36, 430, 686
BseGI GGATG 5 cut(s) 28, 280, 295, 581, 697
BseLI CCNNNNNNNGG 1 cut(s) 669
BseMII CTCAG 3 cut(s) 469, 585, 881
BseNI ACTGG 3 cut(s) 374, 543, 625
BseRI GAGGAG 2 cut(s) 390, 804
BseXI GCAGC 2 cut(s) 660, 808
BsgI GTGCAG 1 cut(s) 100
BshFI GGCC 2 cut(s) 345, 421
BsiHKAI GWGCWC 1 cut(s) 814
BsiHKCI CYCGRG 1 cut(s) 35
BsiSI CCGG 1 cut(s) 233
BslFI GGGAC 3 cut(s) 118, 351, 873
BslI CCNNNNNNNGG 1 cut(s) 669
BsmAI GTCTC 2 cut(s) 723, 772
BsmFI GGGAC 3 cut(s) 118, 351, 873
BsnI GGCC 2 cut(s) 345, 421
BsoBI CYCGRG 1 cut(s) 35
Bsp1286I GDGCHC 2 cut(s) 536, 814
Bsp143I GATC 5 cut(s) 73, 166, 350, 482, 986
BspACI CCGC 1 cut(s) 522
BspANI GGCC 2 cut(s) 345, 421
BspCNI CTCAG 3 cut(s) 468, 584, 880
BspLI GGNNCC 2 cut(s) 484, 702
BspMAI CTGCAG 2 cut(s) 127, 174
BspMI ACCTGC 2 cut(s) 652, 794
BspPI GGATC 2 cut(s) 477, 490
BsrBI CCGCTC 1 cut(s) 524
BsrI ACTGG 3 cut(s) 374, 543, 625
BssECI CCNNGG 4 cut(s) 18, 36, 430, 686
BssMI GATC 5 cut(s) 73, 166, 350, 482, 986
BssT1I CCWWGG 3 cut(s) 18, 430, 686
Bst2UI CCWGG 3 cut(s) 438, 705, 723
Bst4CI ACNGT 3 cut(s) 552, 786, 851
Bst6I CTCTTC 1 cut(s) 415
BstAPI GCANNNNNTGC 1 cut(s) 809
BstC8I GCNNGC 3 cut(s) 381, 385, 801
BstDEI CTNAG 4 cut(s) 182, 455, 571, 867
BstF5I GGATG 5 cut(s) 28, 280, 295, 581, 697
BstKTI GATC 5 cut(s) 76, 169, 353, 485, 989
BstMAI GTCTC 2 cut(s) 723, 772
BstMBI GATC 5 cut(s) 73, 166, 350, 482, 986
BstMWI GCNNNNNNNGC 5 cut(s) 23, 103, 305, 521, 809
BstNI CCWGG 3 cut(s) 438, 705, 723
BstSCI CCNGG 3 cut(s) 436, 703, 721
BstSFI CTRYAG 2 cut(s) 123, 170
BstV1I GCAGC 2 cut(s) 660, 808
BstV2I GAAGAC 1 cut(s) 558
BstX2I RGATCY 1 cut(s) 482
BstXI CCANNNNNNTGG 1 cut(s) 687
BstYI RGATCY 1 cut(s) 482
BsuRI GGCC 2 cut(s) 345, 421
BtrI CACGTC 1 cut(s) 507
BtsCI GGATG 5 cut(s) 28, 280, 295, 581, 697
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 6 cut(s) 83, 120, 155, 381, 463, 618
BveI ACCTGC 2 cut(s) 652, 794
Cac8I GCNNGC 3 cut(s) 381, 385, 801
Cfr13I GGNCC 3 cut(s) 230, 343, 434
Csp6I GTAC 2 cut(s) 88, 179
CspCI CAANNNNNGTGG 2 cut(s) 493, 528
CviAII CATG 4 cut(s) 103, 191, 199, 269
CviQI GTAC 2 cut(s) 88, 179
DdeI CTNAG 4 cut(s) 182, 455, 571, 867
DpnI GATC 5 cut(s) 75, 168, 352, 484, 988
DpnII GATC 5 cut(s) 73, 166, 350, 482, 986
DrdI GACNNNNNNGTC 1 cut(s) 562
DseDI GACNNNNNNGTC 1 cut(s) 562
Eam1104I CTCTTC 1 cut(s) 415
EarI CTCTTC 1 cut(s) 415
Eco130I CCWWGG 3 cut(s) 18, 430, 686
Eco24I GRGCYC 1 cut(s) 536
Eco47I GGWCC 2 cut(s) 230, 434
Eco57I CTGAAG 2 cut(s) 65, 480
Eco88I CYCGRG 1 cut(s) 35
EcoRII CCWGG 3 cut(s) 436, 703, 721
EcoT14I CCWWGG 3 cut(s) 18, 430, 686
EcoT38I GRGCYC 1 cut(s) 536
ErhI CCWWGG 3 cut(s) 18, 430, 686
FaeI CATG 4 cut(s) 106, 194, 202, 272
FaiI YATR 7 cut(s) 104, 192, 200, 270, 771, 846, 991
FalI AAGNNNNNCTT 2 cut(s) 909, 941
FaqI GGGAC 3 cut(s) 118, 351, 873
FatI CATG 4 cut(s) 102, 190, 198, 268
FauI CCCGC 1 cut(s) 529
FbaI TGATCA 1 cut(s) 73
Fnu4HI GCNGC 2 cut(s) 674, 797
FokI GGATG 5 cut(s) 35, 287, 302, 568, 704
FriOI GRGCYC 1 cut(s) 536
Fsp4HI GCNGC 2 cut(s) 674, 797
FspBI CTAG 1 cut(s) 305
GluI GCNGC 2 cut(s) 674, 797
GsuI CTGGAG 4 cut(s) 498, 663, 726, 744
HaeIII GGCC 2 cut(s) 345, 421
HapII CCGG 1 cut(s) 233
Hin1II CATG 4 cut(s) 106, 194, 202, 272
HincII GTYRAC 2 cut(s) 64, 555
HindII GTYRAC 2 cut(s) 64, 555
HinfI GANTC 7 cut(s) 131, 236, 281, 500, 556, 601, 865
HpaII CCGG 1 cut(s) 233
HphI GGTGA 3 cut(s) 214, 473, 600
Hpy166II GTNNAC 5 cut(s) 64, 265, 548, 555, 618
Hpy188I TCNGA 5 cut(s) 45, 286, 499, 777, 870
Hpy188III TCNNGA 3 cut(s) 35, 321, 477
Hpy8I GTNNAC 5 cut(s) 64, 265, 548, 555, 618
Hpy99I CGWCG 1 cut(s) 944
HpyAV CCTTC 5 cut(s) 40, 146, 169, 588, 758
HpyCH4III ACNGT 3 cut(s) 552, 786, 851
HpyCH4IV ACGT 1 cut(s) 506
HpyCH4V TGCA 7 cut(s) 81, 125, 172, 491, 515, 695, 974
HpyF10VI GCNNNNNNNGC 5 cut(s) 23, 103, 305, 521, 809
HpyF3I CTNAG 4 cut(s) 182, 455, 571, 867
HpySE526I ACGT 1 cut(s) 506
Hsp92II CATG 4 cut(s) 106, 194, 202, 272
Ksp22I TGATCA 1 cut(s) 73
Kzo9I GATC 5 cut(s) 73, 166, 350, 482, 986
LmnI GCTCC 4 cut(s) 652, 688, 706, 817
Lsp1109I GCAGC 2 cut(s) 660, 808
LweI GCATC 3 cut(s) 13, 682, 910
MaeI CTAG 1 cut(s) 305
MaeII ACGT 1 cut(s) 506
MalI GATC 5 cut(s) 75, 168, 352, 484, 988
MbiI CCGCTC 1 cut(s) 524
MboI GATC 5 cut(s) 73, 166, 350, 482, 986
MboII GAAGA 7 cut(s) 62, 231, 386, 402, 558, 929, 957
MflI RGATCY 1 cut(s) 482
MhlI GDGCHC 2 cut(s) 536, 814
MluCI AATT 5 cut(s) 293, 328, 444, 538, 883
MlyI GAGTC 5 cut(s) 140, 290, 550, 595, 859
MmeI TCCRAC 1 cut(s) 264
MseI TTAA 2 cut(s) 12, 882
MslI CAYNNNNRTG 1 cut(s) 273
MspI CCGG 1 cut(s) 233
MspR9I CCNGG 3 cut(s) 438, 705, 723
MvaI CCWGG 3 cut(s) 438, 705, 723
MwoI GCNNNNNNNGC 5 cut(s) 23, 103, 305, 521, 809
NdeII GATC 5 cut(s) 73, 166, 350, 482, 986
NlaIII CATG 4 cut(s) 106, 194, 202, 272
NlaIV GGNNCC 2 cut(s) 484, 702
PaqCI CACCTGC 1 cut(s) 794
PfeI GAWTC 2 cut(s) 236, 500
PflFI GACNNNGTC 1 cut(s) 938
PfoI TCCNGGA 2 cut(s) 703, 721
PkrI GCNGC 2 cut(s) 675, 798
PleI GAGTC 5 cut(s) 139, 289, 550, 595, 859
PpsI GAGTC 5 cut(s) 139, 289, 550, 595, 859
Psp6I CCWGG 3 cut(s) 436, 703, 721
PspGI CCWGG 3 cut(s) 436, 703, 721
PspN4I GGNNCC 2 cut(s) 484, 702
PspPI GGNCC 3 cut(s) 230, 343, 434
PstI CTGCAG 2 cut(s) 127, 174
PsuI RGATCY 1 cut(s) 482
PsyI GACNNNGTC 1 cut(s) 938
RsaI GTAC 2 cut(s) 89, 180
RsaNI GTAC 2 cut(s) 88, 179
RseI CAYNNNNRTG 1 cut(s) 273
SaqAI TTAA 2 cut(s) 12, 882
SatI GCNGC 2 cut(s) 674, 797
Sau3AI GATC 5 cut(s) 73, 166, 350, 482, 986
Sau96I GGNCC 3 cut(s) 230, 343, 434
SchI GAGTC 5 cut(s) 140, 290, 550, 595, 859
ScrFI CCNGG 3 cut(s) 438, 705, 723
SduI GDGCHC 2 cut(s) 536, 814
SfaNI GCATC 3 cut(s) 13, 682, 910
SfcI CTRYAG 2 cut(s) 123, 170
SinI GGWCC 2 cut(s) 230, 434
SmiMI CAYNNNNRTG 1 cut(s) 273
Sse9I AATT 5 cut(s) 293, 328, 444, 538, 883
SsiI CCGC 1 cut(s) 522
SspMI CTAG 1 cut(s) 305
StyD4I CCNGG 3 cut(s) 436, 703, 721
StyI CCWWGG 3 cut(s) 18, 430, 686
TaaI ACNGT 3 cut(s) 552, 786, 851
TaiI ACGT 1 cut(s) 509
TaqI TCGA 3 cut(s) 320, 530, 942
TasI AATT 5 cut(s) 293, 328, 444, 538, 883
TfiI GAWTC 2 cut(s) 236, 500
Tru1I TTAA 2 cut(s) 12, 882
Tru9I TTAA 2 cut(s) 12, 882
TscAI CASTG 6 cut(s) 83, 127, 162, 381, 463, 625
TseI GCWGC 2 cut(s) 673, 796
TspDTI ATGAA 4 cut(s) 306, 570, 654, 876
TspRI CASTG 6 cut(s) 83, 127, 162, 381, 463, 625
Tth111I GACNNNGTC 1 cut(s) 938
VpaK11BI GGWCC 2 cut(s) 230, 434
XapI RAATTY 1 cut(s) 293
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.