Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr5

Orthogroup ID

OG0003872

Physical Location & Seq

Genomic Coordinates Forward (+)

509849 .. 515198

Gene Structure 5350 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom05g00450.10

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1572 bp

ATGGCTGGTAATGCTGCAGCTTGTGCTGAGAGGGCAACAAGTGATATGTTGATTGGTCCAGACTGGGCAATAAATATCGAATTATGCGATATTATCAATATGGAGCCTGGGCAAGCAAAAGATGCCCTGAAGATACTCAAGAAGCGCCTGGGCAGCAAAAATCCAAAAATACAACTCCTGGCTCTTTTTGCATTGGAGACTGTGAGCAAGAACTGTGGTGAGAGTGTTTTTCAGCAGATCGTTGAGCGTGATATCTTGCATGAAATGGTCAAAATAGTAAAGAAGAAGCCGGACTTGAATGTTAGGGAAAAGATACTAATTCTGATAGATACATGGCAAGAAGCTTTTGGGGGACCAAGGGGAAGATTTCCCCAGTACCATGCAGCATATAATGAATTGAGGGCTGCTGGAGTTGAATTTCCACCAAGAGAAGAGAACAGTGTACCAATATTTACTCCACCCCAGACCCAGCCAGTAGTTTCCCCTGTTACATCATATGATGATGCTGCCATTCAGGCTTCTCTCCAGTCGGATGCATCTGGCCTTAGCTTGCCAGAGATTCAGAATGCCCGAGGAATTGCAGATGTGTTGATGGAAATGCTTAATGCGTTGGATCCTAAGCATCCTGAGGGTTTGAAGGAAGAAGTGATTGTTGACCTTGTTGATCAGTGCCGTTCATACCAAAAGCGCGTCATGCTTCTTGTGAATGAGACTGCAGATGAGGATCTTCTATGTCAGGGATTGGCACTGAACGATCATCTGCAGCGTGTAATTTCCCGGCATGACGATATTGTGAAAAGAACTACAACTGTAGTACGAGGAGCAGAATCTTCGGTTGTGCCACTTGTGAATGTGAACCATGAGGACGATGAGTCCGAGGATGAATTTTCCCAACTAGCGCATAGGTCATCAAGAGATAATTCACAAGGACAGGGCCAGAGATCAACCAATGCCAAGGCTGAACCTGTGCGAGTCAGCCCACTTCTTCCTCCTCCACCGTCTTCGCAAAGGCCAATCACTCTAGGTCCTGGCCTGATTGATTACCTCAGTGGCGAAGCCTACAAATCTGAAGGATCCTCTGCAGCATCAGAATCCACATCCTTTGCAGTTCCAGTTCATTCCAGCTCCACCTCACGATTGGCTCCACTATCACCCTCACCCCCTTCTTCCCAAAACACAACTCCATCACCTGTATTCGTGGGAAAGCCTGTATATGATGAACCGGCTCCTAGAAGCAAATCTATCGATGGGCTACCTCCTGCTCCTTGGGATACTCAGTCTTTGGGGAATCCTCAGTCTCCTGGCAATGGTCACTCTCCTGTCAGCCTTCCTCCTCCACCTTCCAGGTATAACCAGAGACAACAGTTTTTTGAGCAACAAAACGTTCCAGGCAGCGGATCTGGTTCCTCTGGTGCATCTTATGACAACTTAGTGGGACAGACTCAGAATCTCTCCCTTAATTCGTCTAACCCAACAAAACAAGTGAAAAAGGAAGATGCTCTGTTCAAGGACCTAGTTGATTTTGCAAAAGCCAAATCATCTTCGCCTTCAAACTCGAATAGGTCATTTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0007088 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0009987 GO:0010564 GO:0010639 GO:0010948 GO:0019899 GO:0019900 GO:0019901 GO:0023052 GO:0030276 GO:0033043 GO:0044424 GO:0044464 GO:0045786 GO:0045839 GO:0045930 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051129 GO:0051716 GO:0051726 GO:0051783 GO:0051784 GO:0065007

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

GAT VHS

Protein Analysis

524

Amino Acids

56.69

Weight (kDa)

5.37

Isoelectric Point (pI)

55.64

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003872)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G21380 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970 AT1G76970
fragaria_vesca	FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400 FvH4_2g15400
malus_domestica	MD05G1008800.v1.1 MD10G1007100.v1.1 MD10G1007700.v1.1
prunus_persica	Prupe.8G010900_v2.0.a1 Prupe.8G010900_v2.0.a1
pyrus_communis	pycom05g00450 pycom10g00480
rosa_chinensis	RchiOBHm_Chr6g0278141
rosa_laevigata	RLG00000013238
rosa_multiflora	Rmu_co8265659.1_g000001 Rmu_sc0000791.1_g000057 Rmu_sc0000857.1_g000058
rosa_roxburghii	Rroxscaffold_7G00190500
rosa_rugosa	Rorug06G0114900
rosa_samantha	Rh6AG224900 Rh6BG229000 Rh6CG231500 Rh6DG222300
rosa_wichuraiana	Rw6G019660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	690
AciI	CCGC	1 cut(s)	1395
AclI	AACGTT	1 cut(s)	1383
AclWI	GGATC	6 cut(s)	608, 621, 732, 1068, 1081, 1405
AcsI	RAATTY	2 cut(s)	416, 884
AcuI	CTGAAG	2 cut(s)	149, 1089
AfaI	GTAC	3 cut(s)	377, 444, 816
AfiI	CCNNNNNNNGG	2 cut(s)	1307, 1394
AgsI	TTSAA	5 cut(s)	298, 416, 637, 1507, 1551
AhdI	GACNNNNNGTC	1 cut(s)	871
AjnI	CCWGG	7 cut(s)	106, 147, 177, 1027, 1300, 1343, 1387
AluBI	AGCT	4 cut(s)	20, 344, 549, 1125
AluI	AGCT	4 cut(s)	20, 344, 549, 1125
Alw26I	GTCTC	4 cut(s)	191, 704, 1302, 1351
AlwI	GGATC	6 cut(s)	608, 621, 732, 1068, 1081, 1405
Ama87I	CYCGRG	1 cut(s)	570
AoxI	GGCC	4 cut(s)	541, 934, 1010, 1030
ApeKI	GCWGC	9 cut(s)	14, 17, 153, 383, 404, 506, 763, 1082, 1392
ApoI	RAATTY	2 cut(s)	416, 884
AspLEI	GCGC	3 cut(s)	147, 690, 901
AspS9I	GGNCC	5 cut(s)	56, 353, 934, 1025, 1510
AsuC2I	CCSGG	1 cut(s)	778
AsuHPI	GGTGA	4 cut(s)	230, 1143, 1149, 1179
AvaI	CYCGRG	1 cut(s)	570
AvaII	GGWCC	4 cut(s)	56, 353, 1025, 1510
AxyI	CCTNAGG	1 cut(s)	627
BamHI	GGATCC	2 cut(s)	613, 1073
BbsI	GAAGAC	1 cut(s)	993
BbvI	GCAGC	8 cut(s)	29, 165, 391, 395, 493, 775, 1094, 1404
BccI	CCATC	3 cut(s)	586, 1192, 1241
BceAI	ACGGC	1 cut(s)	657
BcgI	CGANNNNNNTGC	4 cut(s)	813, 847, 1225, 1259
BciT130I	CCWGG	7 cut(s)	108, 149, 179, 1029, 1302, 1345, 1389
BciVI	GTATCC	1 cut(s)	1264
BclI	TGATCA	1 cut(s)	664
BcnI	CCSGG	1 cut(s)	778
BcoDI	GTCTC	4 cut(s)	191, 704, 1302, 1351
BfaI	CTAG	4 cut(s)	896, 1022, 1230, 1514
BfmI	CTRYAG	5 cut(s)	15, 714, 761, 810, 1080
BfoI	RGCGCY	1 cut(s)	148
BfuI	GTATCC	1 cut(s)	1264
BglI	GCCNNNNNGGC	1 cut(s)	515
BisI	GCNGC	9 cut(s)	15, 18, 154, 384, 405, 507, 764, 1083, 1393
BlsI	GCNGC	9 cut(s)	16, 19, 155, 385, 406, 508, 765, 1084, 1394
Bme1390I	CCNGG	8 cut(s)	108, 149, 179, 778, 1029, 1302, 1345, 1389
Bme18I	GGWCC	4 cut(s)	56, 353, 1025, 1510
BmeRI	GACNNNNNGTC	1 cut(s)	871
BmeT110I	CYCGRG	1 cut(s)	570
BmgT120I	GGNCC	5 cut(s)	56, 353, 934, 1025, 1510
BmiI	GGNNCC	7 cut(s)	105, 354, 615, 1075, 1143, 1227, 1405
BmrFI	CCNGG	8 cut(s)	108, 149, 179, 778, 1029, 1302, 1345, 1389
BmrI	ACTGGG	2 cut(s)	73, 367
BmsI	GCATC	8 cut(s)	112, 493, 523, 545, 631, 1094, 1424, 1486
BmuI	ACTGGG	2 cut(s)	73, 367
BpiI	GAAGAC	1 cut(s)	993
BpmI	CTGGAG	2 cut(s)	429, 509
Bpu10I	CCTNAGC	2 cut(s)	545, 618
BpuEI	CTTGAG	1 cut(s)	122
BpuMI	CCSGG	1 cut(s)	778
Bsa29I	ATCGAT	1 cut(s)	1245
BsaBI	GATNNNNATC	1 cut(s)	723
BsaJI	CCNNGG	7 cut(s)	107, 148, 356, 571, 876, 954, 1265
Bsc4I	CCNNNNNNNGG	2 cut(s)	1307, 1394
Bse118I	RCCGGY	1 cut(s)	1222
Bse1I	ACTGG	5 cut(s)	68, 373, 473, 526, 1112
Bse21I	CCTNAGG	1 cut(s)	627
Bse3DI	GCAATG	1 cut(s)	1312
Bse8I	GATNNNNATC	1 cut(s)	723
BseBI	CCWGG	7 cut(s)	108, 149, 179, 1029, 1302, 1345, 1389
BseCI	ATCGAT	1 cut(s)	1245
BseDI	CCNNGG	7 cut(s)	107, 148, 356, 571, 876, 954, 1265
BseGI	GGATG	4 cut(s)	538, 622, 886, 1097
BseJI	GATNNNNATC	1 cut(s)	723
BseLI	CCNNNNNNNGG	2 cut(s)	1307, 1394
BseMI	GCAATG	1 cut(s)	1312
BseMII	CTCAG	6 cut(s)	18, 618, 1060, 1289, 1307, 1457
BseNI	ACTGG	5 cut(s)	68, 373, 473, 526, 1112
BseRI	GAGGAG	3 cut(s)	834, 981, 1323
BseXI	GCAGC	8 cut(s)	29, 165, 391, 395, 493, 775, 1094, 1404
BseYI	CCCAGC	1 cut(s)	468
Bsh1236I	CGCG	1 cut(s)	690
BshFI	GGCC	4 cut(s)	543, 936, 1012, 1032
BshVI	ATCGAT	1 cut(s)	1245
BsiHKCI	CYCGRG	1 cut(s)	570
BsiSI	CCGG	3 cut(s)	290, 778, 1223
BslFI	GGGAC	2 cut(s)	366, 1449
BslI	CCNNNNNNNGG	2 cut(s)	1307, 1394
BsmAI	GTCTC	4 cut(s)	191, 704, 1302, 1351
BsmFI	GGGAC	2 cut(s)	366, 1449
BsmI	GAATGC	1 cut(s)	571
BsnI	GGCC	4 cut(s)	543, 936, 1012, 1032
BsoBI	CYCGRG	1 cut(s)	570
Bsp143I	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
BspACI	CCGC	1 cut(s)	1395
BspANI	GGCC	4 cut(s)	543, 936, 1012, 1032
BspCNI	CTCAG	6 cut(s)	19, 619, 1059, 1288, 1306, 1456
BspDI	ATCGAT	1 cut(s)	1245
BspFNI	CGCG	1 cut(s)	690
BspLI	GGNNCC	7 cut(s)	105, 354, 615, 1075, 1143, 1227, 1405
BspMAI	CTGCAG	4 cut(s)	19, 718, 765, 1084
BspPI	GGATC	6 cut(s)	608, 621, 732, 1068, 1081, 1405
BsrDI	GCAATG	1 cut(s)	1312
BsrFI	RCCGGY	1 cut(s)	1222
BsrI	ACTGG	5 cut(s)	68, 373, 473, 526, 1112
BssAI	RCCGGY	1 cut(s)	1222
BssECI	CCNNGG	7 cut(s)	107, 148, 356, 571, 876, 954, 1265
BssMI	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
BssT1I	CCWWGG	3 cut(s)	356, 954, 1265
Bst2UI	CCWGG	7 cut(s)	108, 149, 179, 1029, 1302, 1345, 1389
Bst4CI	ACNGT	6 cut(s)	202, 215, 440, 811, 999, 1365
Bst6I	CTCTTC	1 cut(s)	426
BstAPI	GCANNNNNTGC	2 cut(s)	23, 122
BstC8I	GCNNGC	2 cut(s)	114, 551
BstDEI	CTNAG	9 cut(s)	27, 545, 618, 627, 1046, 1275, 1293, 1429, 1443
BstF5I	GGATG	4 cut(s)	538, 622, 886, 1097
BstFNI	CGCG	1 cut(s)	690
BstH2I	RGCGCY	1 cut(s)	148
BstHHI	GCGC	3 cut(s)	147, 690, 901
BstKTI	GATC	8 cut(s)	240, 616, 667, 727, 757, 944, 1076, 1400
BstMAI	GTCTC	4 cut(s)	191, 704, 1302, 1351
BstMBI	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
BstMWI	GCNNNNNNNGC	8 cut(s)	11, 23, 32, 122, 153, 188, 515, 694
BstNI	CCWGG	7 cut(s)	108, 149, 179, 1029, 1302, 1345, 1389
BstSCI	CCNGG	8 cut(s)	106, 147, 177, 776, 1027, 1300, 1343, 1387
BstSFI	CTRYAG	5 cut(s)	15, 714, 761, 810, 1080
BstUI	CGCG	1 cut(s)	690
BstV1I	GCAGC	8 cut(s)	29, 165, 391, 395, 493, 775, 1094, 1404
BstV2I	GAAGAC	1 cut(s)	993
BstX2I	RGATCY	4 cut(s)	613, 724, 1073, 1397
BstYI	RGATCY	4 cut(s)	613, 724, 1073, 1397
Bsu15I	ATCGAT	1 cut(s)	1245
Bsu36I	CCTNAGG	1 cut(s)	627
BsuI	GTATCC	1 cut(s)	1264
BsuRI	GGCC	4 cut(s)	543, 936, 1012, 1032
BsuTUI	ATCGAT	1 cut(s)	1245
BtsCI	GGATG	4 cut(s)	538, 622, 886, 1097
BtsIMutI	CAGTG	4 cut(s)	445, 674, 746, 1054
Cac8I	GCNNGC	2 cut(s)	114, 551
CfoI	GCGC	3 cut(s)	147, 690, 901
Cfr10I	RCCGGY	1 cut(s)	1222
Cfr13I	GGNCC	5 cut(s)	56, 353, 934, 1025, 1510
ClaI	ATCGAT	1 cut(s)	1245
CseI	GACGC	1 cut(s)	679
Csp6I	GTAC	3 cut(s)	376, 443, 815
CspCI	CAANNNNNGTGG	4 cut(s)	196, 231, 1084, 1119
CviAII	CATG	6 cut(s)	260, 333, 380, 694, 782, 860
CviQI	GTAC	3 cut(s)	376, 443, 815
DdeI	CTNAG	9 cut(s)	27, 545, 618, 627, 1046, 1275, 1293, 1429, 1443
DpnI	GATC	8 cut(s)	239, 615, 666, 726, 756, 943, 1075, 1399
DpnII	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
DriI	GACNNNNNGTC	1 cut(s)	871
Eam1104I	CTCTTC	1 cut(s)	426
Eam1105I	GACNNNNNGTC	1 cut(s)	871
EarI	CTCTTC	1 cut(s)	426
Eco130I	CCWWGG	3 cut(s)	356, 954, 1265
Eco32I	GATATC	1 cut(s)	253
Eco47I	GGWCC	4 cut(s)	56, 353, 1025, 1510
Eco57I	CTGAAG	2 cut(s)	149, 1089
Eco81I	CCTNAGG	1 cut(s)	627
Eco88I	CYCGRG	1 cut(s)	570
EcoO109I	RGGNCCY	2 cut(s)	1025, 1510
EcoRII	CCWGG	7 cut(s)	106, 147, 177, 1027, 1300, 1343, 1387
EcoRV	GATATC	1 cut(s)	253
EcoT14I	CCWWGG	3 cut(s)	356, 954, 1265
EcoT22I	ATGCAT	1 cut(s)	538
ErhI	CCWWGG	3 cut(s)	356, 954, 1265
FaeI	CATG	6 cut(s)	263, 336, 383, 697, 785, 863
FalI	AAGNNNNNCTT	2 cut(s)	278, 310
FaqI	GGGAC	2 cut(s)	366, 1449
FatI	CATG	6 cut(s)	259, 332, 379, 693, 781, 859
FauNDI	CATATG	1 cut(s)	496
FbaI	TGATCA	1 cut(s)	664
Fnu4HI	GCNGC	9 cut(s)	15, 18, 154, 384, 405, 507, 764, 1083, 1393
FokI	GGATG	4 cut(s)	545, 609, 893, 1084
Fsp4HI	GCNGC	9 cut(s)	15, 18, 154, 384, 405, 507, 764, 1083, 1393
FspBI	CTAG	4 cut(s)	896, 1022, 1230, 1514
GlaI	GCGC	3 cut(s)	146, 689, 900
GluI	GCNGC	9 cut(s)	15, 18, 154, 384, 405, 507, 764, 1083, 1393
GsaI	CCCAGC	1 cut(s)	472
GsuI	CTGGAG	2 cut(s)	429, 509
HaeII	RGCGCY	1 cut(s)	148
HaeIII	GGCC	4 cut(s)	543, 936, 1012, 1032
HapII	CCGG	3 cut(s)	290, 778, 1223
HgaI	GACGC	1 cut(s)	679
HhaI	GCGC	3 cut(s)	147, 690, 901
Hin1II	CATG	6 cut(s)	263, 336, 383, 697, 785, 863
Hin6I	GCGC	3 cut(s)	145, 688, 899
HinP1I	GCGC	3 cut(s)	145, 688, 899
HincII	GTYRAC	1 cut(s)	655
HindII	GTYRAC	1 cut(s)	655
HindIII	AAGCTT	1 cut(s)	342
HinfI	GANTC	8 cut(s)	559, 827, 872, 972, 1091, 1288, 1441, 1447
HpaII	CCGG	3 cut(s)	290, 778, 1223
HphI	GGTGA	4 cut(s)	230, 1143, 1149, 1179
Hpy166II	GTNNAC	3 cut(s)	443, 655, 856
Hpy188I	TCNGA	7 cut(s)	324, 532, 564, 877, 1069, 1090, 1446
Hpy188III	TCNNGA	5 cut(s)	59, 139, 626, 912, 1134
Hpy8I	GTNNAC	3 cut(s)	443, 655, 856
HpyAV	CCTTC	6 cut(s)	631, 1064, 1173, 1337, 1350, 1557
HpyCH4III	ACNGT	6 cut(s)	202, 215, 440, 811, 999, 1365
HpyCH4IV	ACGT	1 cut(s)	1383
HpyF10VI	GCNNNNNNNGC	8 cut(s)	11, 23, 32, 122, 153, 188, 515, 694
HpyF3I	CTNAG	9 cut(s)	27, 545, 618, 627, 1046, 1275, 1293, 1429, 1443
HpySE526I	ACGT	1 cut(s)	1383
Hsp92II	CATG	6 cut(s)	263, 336, 383, 697, 785, 863
HspAI	GCGC	3 cut(s)	145, 688, 899
Ksp22I	TGATCA	1 cut(s)	664
Kzo9I	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
LmnI	GCTCC	6 cut(s)	103, 821, 1130, 1147, 1231, 1267
Lsp1109I	GCAGC	8 cut(s)	29, 165, 391, 395, 493, 775, 1094, 1404
LweI	GCATC	8 cut(s)	112, 493, 523, 545, 631, 1094, 1424, 1486
MaeI	CTAG	4 cut(s)	896, 1022, 1230, 1514
MaeII	ACGT	1 cut(s)	1383
MaeIII	GTNAC	2 cut(s)	487, 1310
MalI	GATC	8 cut(s)	239, 615, 666, 726, 756, 943, 1075, 1399
MboI	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
MflI	RGATCY	4 cut(s)	613, 724, 1073, 1397
MluCI	AATT	9 cut(s)	80, 318, 395, 416, 576, 771, 884, 919, 1459
MlyI	GAGTC	3 cut(s)	881, 981, 1435
MmeI	TCCRAC	2 cut(s)	510, 591
Mph1103I	ATGCAT	1 cut(s)	538
MseI	TTAA	2 cut(s)	603, 1458
MspA1I	CMGCKG	1 cut(s)	1395
MspI	CCGG	3 cut(s)	290, 778, 1223
MspR9I	CCNGG	8 cut(s)	108, 149, 179, 778, 1029, 1302, 1345, 1389
Mva1269I	GAATGC	1 cut(s)	571
MvaI	CCWGG	7 cut(s)	108, 149, 179, 1029, 1302, 1345, 1389
MvnI	CGCG	1 cut(s)	690
MwoI	GCNNNNNNNGC	8 cut(s)	11, 23, 32, 122, 153, 188, 515, 694
NciI	CCSGG	1 cut(s)	778
NdeI	CATATG	1 cut(s)	496
NdeII	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
NlaIII	CATG	6 cut(s)	263, 336, 383, 697, 785, 863
NlaIV	GGNNCC	7 cut(s)	105, 354, 615, 1075, 1143, 1227, 1405
NmuCI	GTSAC	1 cut(s)	1310
NsiI	ATGCAT	1 cut(s)	538
PcsI	WCGNNNNNNNCGW	2 cut(s)	84, 873
PctI	GAATGC	1 cut(s)	571
PfeI	GAWTC	5 cut(s)	559, 827, 1091, 1288, 1447
PkrI	GCNGC	9 cut(s)	16, 19, 155, 385, 406, 508, 765, 1084, 1394
PleI	GAGTC	3 cut(s)	880, 980, 1435
PpsI	GAGTC	3 cut(s)	880, 980, 1435
PpuMI	RGGWCCY	2 cut(s)	1025, 1510
Psp1406I	AACGTT	1 cut(s)	1383
Psp5II	RGGWCCY	2 cut(s)	1025, 1510
Psp6I	CCWGG	7 cut(s)	106, 147, 177, 1027, 1300, 1343, 1387
PspFI	CCCAGC	1 cut(s)	468
PspGI	CCWGG	7 cut(s)	106, 147, 177, 1027, 1300, 1343, 1387
PspN4I	GGNNCC	7 cut(s)	105, 354, 615, 1075, 1143, 1227, 1405
PspPI	GGNCC	5 cut(s)	56, 353, 934, 1025, 1510
PspPPI	RGGWCCY	2 cut(s)	1025, 1510
PstI	CTGCAG	4 cut(s)	19, 718, 765, 1084
PsuI	RGATCY	4 cut(s)	613, 724, 1073, 1397
RsaI	GTAC	3 cut(s)	377, 444, 816
RsaNI	GTAC	3 cut(s)	376, 443, 815
SaqAI	TTAA	2 cut(s)	603, 1458
SatI	GCNGC	9 cut(s)	15, 18, 154, 384, 405, 507, 764, 1083, 1393
Sau3AI	GATC	8 cut(s)	237, 613, 664, 724, 754, 941, 1073, 1397
Sau96I	GGNCC	5 cut(s)	56, 353, 934, 1025, 1510
SchI	GAGTC	3 cut(s)	881, 981, 1435
ScrFI	CCNGG	8 cut(s)	108, 149, 179, 778, 1029, 1302, 1345, 1389
SfaNI	GCATC	8 cut(s)	112, 493, 523, 545, 631, 1094, 1424, 1486
SfcI	CTRYAG	5 cut(s)	15, 714, 761, 810, 1080
SinI	GGWCC	4 cut(s)	56, 353, 1025, 1510
SmlI	CTYRAG	1 cut(s)	137
SmoI	CTYRAG	1 cut(s)	137
Sse9I	AATT	9 cut(s)	80, 318, 395, 416, 576, 771, 884, 919, 1459
SsiI	CCGC	1 cut(s)	1395
SspI	AATATT	1 cut(s)	450
SspMI	CTAG	4 cut(s)	896, 1022, 1230, 1514
StyD4I	CCNGG	8 cut(s)	106, 147, 177, 776, 1027, 1300, 1343, 1387
StyI	CCWWGG	3 cut(s)	356, 954, 1265
TaaI	ACNGT	6 cut(s)	202, 215, 440, 811, 999, 1365
TaiI	ACGT	1 cut(s)	1386
TaqI	TCGA	3 cut(s)	78, 1245, 1556
TasI	AATT	9 cut(s)	80, 318, 395, 416, 576, 771, 884, 919, 1459
TfiI	GAWTC	5 cut(s)	559, 827, 1091, 1288, 1447
Tru1I	TTAA	2 cut(s)	603, 1458
Tru9I	TTAA	2 cut(s)	603, 1458
TscAI	CASTG	4 cut(s)	445, 674, 753, 1054
TseFI	GTSAC	1 cut(s)	1310
TseI	GCWGC	9 cut(s)	14, 17, 153, 383, 404, 506, 763, 1082, 1392
Tsp45I	GTSAC	1 cut(s)	1310
TspDTI	ATGAA	6 cut(s)	276, 408, 666, 897, 1106, 1233
TspRI	CASTG	4 cut(s)	445, 674, 753, 1054
VpaK11BI	GGWCC	4 cut(s)	56, 353, 1025, 1510
XapI	RAATTY	2 cut(s)	416, 884
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1135
XspI	CTAG	4 cut(s)	896, 1022, 1230, 1514
Zsp2I	ATGCAT	1 cut(s)	538

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.