MD05G1008800.v1.1

TOM1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0003872
Physical Location & Seq
Forward (+)
1882552 .. 1886211
3660 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1008800.v1.1.491

Sequence Viewer

Length: 555 bp
ATGGCTGGTAATGCTGCAGCTTGTGCTGAGAGGGCAACAAGTGATATGTTGATTGGTCCAGACTGGGCAATAAATATCGAATTATGCGATATCATCAATGTGGAGCCTGGGCAAGCAAAAGATGCCTTGAAGATACTTAAGAAGCGCCTGGGCAGCAAAAATCCAAAAATACAACTCCTGGCTCTTTTTGCATTGGAGACTGTGAGCAAGAACTGTGGTGAGAATGTTTTTCAGCAGATCGTTGAGCGTGATATCTTGCATGAAATGGTCAAAATAGTAAAGAAGAAGCCGGACTTGAATGTTAGGGAAAAGATACTACTTCTGATAGATACATGGCAAGAAGCTTTTGGGGGACCAAGGGGAAGATTTCCCCAGTACCATGCAGCATATAATGAATTGAGGGCTGCTGGAGTTGAATTTCCACCAAGAGAAGAGAACAGTGTACCAATATTTACTCCACCCCAGACCCAGCCAGTCGTTTCCCCTGTTACATCATATGATGATGCTGCCATTCAGGCTTCTCTCCAGTCGGATGCATCTGGCCTTAGGTACTAG

Protein Analysis

185

Amino Acids

20.33

Weight (kDa)

5.76

Isoelectric Point (pI)

38.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VHS PF00790 10 - 118 4.1e-29 VHS domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 416
AfaI GTAC 3 cut(s) 377, 444, 551
AflII CTTAAG 1 cut(s) 137
AgsI TTSAA 3 cut(s) 130, 298, 416
AjnI CCWGG 3 cut(s) 106, 147, 177
AluBI AGCT 2 cut(s) 20, 344
AluI AGCT 2 cut(s) 20, 344
Alw26I GTCTC 1 cut(s) 191
AoxI GGCC 1 cut(s) 541
ApeKI GCWGC 6 cut(s) 14, 17, 153, 383, 404, 506
ApoI RAATTY 1 cut(s) 416
AspLEI GCGC 1 cut(s) 147
AspS9I GGNCC 2 cut(s) 56, 353
AsuHPI GGTGA 1 cut(s) 230
AvaII GGWCC 2 cut(s) 56, 353
AxyI CCTNAGG 1 cut(s) 545
BbvI GCAGC 5 cut(s) 29, 165, 391, 395, 493
BciT130I CCWGG 3 cut(s) 108, 149, 179
BcoDI GTCTC 1 cut(s) 191
BfaI CTAG 1 cut(s) 553
BfmI CTRYAG 1 cut(s) 15
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 137
BglI GCCNNNNNGGC 1 cut(s) 515
BisI GCNGC 6 cut(s) 15, 18, 154, 384, 405, 507
BlsI GCNGC 6 cut(s) 16, 19, 155, 385, 406, 508
Bme1390I CCNGG 3 cut(s) 108, 149, 179
Bme18I GGWCC 2 cut(s) 56, 353
BmgT120I GGNCC 2 cut(s) 56, 353
BmiI GGNNCC 2 cut(s) 105, 354
BmrFI CCNGG 3 cut(s) 108, 149, 179
BmrI ACTGGG 2 cut(s) 73, 367
BmsI GCATC 4 cut(s) 112, 493, 523, 545
BmuI ACTGGG 2 cut(s) 73, 367
BpmI CTGGAG 2 cut(s) 429, 509
BsaJI CCNNGG 3 cut(s) 107, 148, 356
Bse1I ACTGG 4 cut(s) 68, 373, 473, 526
Bse21I CCTNAGG 1 cut(s) 545
BseBI CCWGG 3 cut(s) 108, 149, 179
BseDI CCNNGG 3 cut(s) 107, 148, 356
BseGI GGATG 1 cut(s) 538
BseMII CTCAG 1 cut(s) 18
BseNI ACTGG 4 cut(s) 68, 373, 473, 526
BseXI GCAGC 5 cut(s) 29, 165, 391, 395, 493
BseYI CCCAGC 1 cut(s) 468
BshFI GGCC 1 cut(s) 543
BsiSI CCGG 1 cut(s) 290
BslFI GGGAC 1 cut(s) 366
BsmAI GTCTC 1 cut(s) 191
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 543
Bsp143I GATC 1 cut(s) 237
BspANI GGCC 1 cut(s) 543
BspCNI CTCAG 1 cut(s) 19
BspLI GGNNCC 2 cut(s) 105, 354
BspMAI CTGCAG 1 cut(s) 19
BspTI CTTAAG 1 cut(s) 137
BsrI ACTGG 4 cut(s) 68, 373, 473, 526
BssECI CCNNGG 3 cut(s) 107, 148, 356
BssMI GATC 1 cut(s) 237
BssT1I CCWWGG 1 cut(s) 356
Bst2UI CCWGG 3 cut(s) 108, 149, 179
Bst4CI ACNGT 3 cut(s) 202, 215, 440
Bst6I CTCTTC 1 cut(s) 426
BstAFI CTTAAG 1 cut(s) 137
BstAPI GCANNNNNTGC 2 cut(s) 23, 122
BstC8I GCNNGC 1 cut(s) 114
BstDEI CTNAG 2 cut(s) 27, 545
BstF5I GGATG 1 cut(s) 538
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 1 cut(s) 147
BstKTI GATC 1 cut(s) 240
BstMAI GTCTC 1 cut(s) 191
BstMBI GATC 1 cut(s) 237
BstMWI GCNNNNNNNGC 7 cut(s) 11, 23, 32, 122, 153, 188, 515
BstNI CCWGG 3 cut(s) 108, 149, 179
BstSCI CCNGG 3 cut(s) 106, 147, 177
BstSFI CTRYAG 1 cut(s) 15
BstV1I GCAGC 5 cut(s) 29, 165, 391, 395, 493
Bsu36I CCTNAGG 1 cut(s) 545
BsuRI GGCC 1 cut(s) 543
BtsCI GGATG 1 cut(s) 538
BtsIMutI CAGTG 1 cut(s) 445
Cac8I GCNNGC 1 cut(s) 114
CfoI GCGC 1 cut(s) 147
Cfr13I GGNCC 2 cut(s) 56, 353
Csp6I GTAC 3 cut(s) 376, 443, 550
CspCI CAANNNNNGTGG 2 cut(s) 196, 231
CviAII CATG 3 cut(s) 260, 333, 380
CviQI GTAC 3 cut(s) 376, 443, 550
DdeI CTNAG 2 cut(s) 27, 545
DpnI GATC 1 cut(s) 239
DpnII GATC 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 426
EarI CTCTTC 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 356
Eco32I GATATC 2 cut(s) 91, 253
Eco47I GGWCC 2 cut(s) 56, 353
Eco81I CCTNAGG 1 cut(s) 545
EcoRII CCWGG 3 cut(s) 106, 147, 177
EcoRV GATATC 2 cut(s) 91, 253
EcoT14I CCWWGG 1 cut(s) 356
EcoT22I ATGCAT 1 cut(s) 538
ErhI CCWWGG 1 cut(s) 356
FaeI CATG 3 cut(s) 263, 336, 383
FaiI YATR 9 cut(s) 47, 85, 261, 334, 381, 388, 390, 496, 498
FalI AAGNNNNNCTT 2 cut(s) 278, 310
FaqI GGGAC 1 cut(s) 366
FatI CATG 3 cut(s) 259, 332, 379
FauNDI CATATG 1 cut(s) 496
Fnu4HI GCNGC 6 cut(s) 15, 18, 154, 384, 405, 507
FokI GGATG 1 cut(s) 545
Fsp4HI GCNGC 6 cut(s) 15, 18, 154, 384, 405, 507
FspBI CTAG 1 cut(s) 553
GlaI GCGC 1 cut(s) 146
GluI GCNGC 6 cut(s) 15, 18, 154, 384, 405, 507
GsaI CCCAGC 1 cut(s) 472
GsuI CTGGAG 2 cut(s) 429, 509
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 1 cut(s) 543
HapII CCGG 1 cut(s) 290
HhaI GCGC 1 cut(s) 147
Hin1II CATG 3 cut(s) 263, 336, 383
Hin6I GCGC 1 cut(s) 145
HinP1I GCGC 1 cut(s) 145
HindIII AAGCTT 1 cut(s) 342
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 230
Hpy166II GTNNAC 1 cut(s) 443
Hpy188I TCNGA 2 cut(s) 324, 532
Hpy188III TCNNGA 1 cut(s) 59
Hpy8I GTNNAC 1 cut(s) 443
HpyCH4III ACNGT 3 cut(s) 202, 215, 440
HpyCH4V TGCA 5 cut(s) 17, 191, 259, 383, 536
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 23, 32, 122, 153, 188, 515
HpyF3I CTNAG 2 cut(s) 27, 545
Hsp92II CATG 3 cut(s) 263, 336, 383
HspAI GCGC 1 cut(s) 145
Kzo9I GATC 1 cut(s) 237
LmnI GCTCC 1 cut(s) 103
Lsp1109I GCAGC 5 cut(s) 29, 165, 391, 395, 493
LweI GCATC 4 cut(s) 112, 493, 523, 545
MaeI CTAG 1 cut(s) 553
MaeIII GTNAC 1 cut(s) 487
MalI GATC 1 cut(s) 239
MboI GATC 1 cut(s) 237
MboII GAAGA 4 cut(s) 142, 295, 375, 443
MluCI AATT 3 cut(s) 80, 395, 416
MmeI TCCRAC 1 cut(s) 510
MnlI CCTC 2 cut(s) 24, 393
Mph1103I ATGCAT 1 cut(s) 538
MseI TTAA 1 cut(s) 138
MslI CAYNNNNRTG 1 cut(s) 98
MspCI CTTAAG 1 cut(s) 137
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 3 cut(s) 108, 149, 179
MvaI CCWGG 3 cut(s) 108, 149, 179
MwoI GCNNNNNNNGC 7 cut(s) 11, 23, 32, 122, 153, 188, 515
NdeI CATATG 1 cut(s) 496
NdeII GATC 1 cut(s) 237
NlaIII CATG 3 cut(s) 263, 336, 383
NlaIV GGNNCC 2 cut(s) 105, 354
NsiI ATGCAT 1 cut(s) 538
PcsI WCGNNNNNNNCGW 1 cut(s) 84
PkrI GCNGC 6 cut(s) 16, 19, 155, 385, 406, 508
Psp6I CCWGG 3 cut(s) 106, 147, 177
PspFI CCCAGC 1 cut(s) 468
PspGI CCWGG 3 cut(s) 106, 147, 177
PspN4I GGNNCC 2 cut(s) 105, 354
PspPI GGNCC 2 cut(s) 56, 353
PstI CTGCAG 1 cut(s) 19
RsaI GTAC 3 cut(s) 377, 444, 551
RsaNI GTAC 3 cut(s) 376, 443, 550
RseI CAYNNNNRTG 1 cut(s) 98
SaqAI TTAA 1 cut(s) 138
SatI GCNGC 6 cut(s) 15, 18, 154, 384, 405, 507
Sau3AI GATC 1 cut(s) 237
Sau96I GGNCC 2 cut(s) 56, 353
ScrFI CCNGG 3 cut(s) 108, 149, 179
SetI ASST 3 cut(s) 22, 346, 551
SfaNI GCATC 4 cut(s) 112, 493, 523, 545
SfcI CTRYAG 1 cut(s) 15
SinI GGWCC 2 cut(s) 56, 353
SmiMI CAYNNNNRTG 1 cut(s) 98
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
Sse9I AATT 3 cut(s) 80, 395, 416
SspI AATATT 1 cut(s) 450
SspMI CTAG 1 cut(s) 553
StyD4I CCNGG 3 cut(s) 106, 147, 177
StyI CCWWGG 1 cut(s) 356
TaaI ACNGT 3 cut(s) 202, 215, 440
TaqI TCGA 1 cut(s) 78
TasI AATT 3 cut(s) 80, 395, 416
Tru1I TTAA 1 cut(s) 138
Tru9I TTAA 1 cut(s) 138
TscAI CASTG 1 cut(s) 445
TseI GCWGC 6 cut(s) 14, 17, 153, 383, 404, 506
TspDTI ATGAA 2 cut(s) 276, 408
TspRI CASTG 1 cut(s) 445
Vha464I CTTAAG 1 cut(s) 137
VpaK11BI GGWCC 2 cut(s) 56, 353
XapI RAATTY 1 cut(s) 416
XspI CTAG 1 cut(s) 553
Zsp2I ATGCAT 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.