AT1G80400

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0005617
Physical Location & Seq
Forward (+)
30225176 .. 30227575
2400 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G80400.1

Sequence Viewer

Length: 1224 bp
ATGGATGTTTCTTCCATTGTGCGAAATACAACCCTAACTGATCAAGATCCTTTGTTAATGGACCACGGTAACCGCGAGCACATCGTTGACATTACAAGCAATGACGAGGAGGACTCTTCATCAAGATCTTCTCTCGATGAGCTTACTCCTCAAGGTAACTCACATCAAGGTGAAGAGACAGTATTATCATCATCAAATGCTACACAACAACACCCAACTCATCCATCTCCTCCTCAGCAAAGACCAACTTCTTCTAGGAGAGGAGATAACGGGAATGGTCGCCGTACTAGGAGTCCATTGAATTCTGGCCTCTGGATCTCAGTTGAGTTAGTTGTCACTGTCGCTCAGATTGTTGCAGCCATTGTAGTTATGGTTCTGGCTAAAGATGAACATCCTGAAGCGCCATTATTTACTTGGGTTATTGGATATACTTGTGGCTGTCTAGCCACTCTCCCTATTCTCTATTGGCGTTTTCGCACTTATAATCGAGCTACAGGGCAAGACTCTTCACAACGTGCTACCTCTTCTCAAGGCAATAGTGAGTCCACACCATATACAGCAGTCTCAGTTGCTCAAGCAGCAGATGAAGAAAATAGCACTGGCGTGAGTGCAGCACCTAGAAACAATCAAGTTGGGGAAAGCTTAAGAACAAGGCTCAACGGGCTAGTGGACCACTTCAAGATGGCGATTGATTGTTTCTTTGCGGTGTGGTTTGTTGTGGGGAATGTATGGATATTTGGAGGTCACTCATCTCCTTCTGATTCTCCTAAACTGTACAGGTTATGCATAGCGTTCCTTACTTTCAGTTGCATTGGATATGCAATGCCATTCATACTATGTGCAACCATATGTTGTTGTTTGCCTTGCTTAATCTCGGTTCTCGGATTTCGAGAGAACTTCTCACAAACTAGAGGAGCCACTGCCGAGGCCATCAACGCATTGCCTGTCTACAGGTTCAAATCAAAAAGTAGAAATGATTTGGAGTTTTCAGAAGAAGGTGAAGGTGGATTTCTTCTCTTGGGGTCTCAGAAGAAACGTCTTATATCAGGCGAGGATGCCAGCTGTTGCATCTGCTTGACTAGATATGGAGACGACGAACAAGTTAGAGAGCTGCCATGCTCACATGTCTTCCATGTAGACTGTGTAGACAAATGGCTTAAAATCAATGCGACTTGCCCTCTCTGCAAAAATGAGGTTGGAGAAAGCAGCAGTGCTTCCTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2

Protein Analysis

407

Amino Acids

44.55

Weight (kDa)

5.42

Isoelectric Point (pI)

43.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 354 - 396 3.1e-15 Ring finger domain
zf-rbx1 PF12678 354 - 396 6.7e-09 RING-H2 zinc finger domain
zf-RING_11 PF17123 355 - 382 9.5e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 355 - 395 3.2e-06 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 355 - 395 5.1e-06 Zinc finger, C3HC4 type (RING finger)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 483
AccI GTMKAC 3 cut(s) 948, 1137, 1146
AccII CGCG 1 cut(s) 75
AciI CCGC 2 cut(s) 73, 704
AclWI GGATC 2 cut(s) 41, 323
AcsI RAATTY 1 cut(s) 301
AcuI CTGAAG 1 cut(s) 417
AdeI CACNNNGTG 1 cut(s) 515
AfaI GTAC 2 cut(s) 286, 776
AflII CTTAAG 1 cut(s) 643
AflIII ACRYGT 1 cut(s) 1123
AgsI TTSAA 3 cut(s) 301, 679, 958
AleI CACNNNNGTG 1 cut(s) 602
AluBI AGCT 5 cut(s) 142, 491, 642, 1062, 1111
AluI AGCT 5 cut(s) 142, 491, 642, 1062, 1111
Alw21I GWGCWC 1 cut(s) 81
Alw26I GTCTC 4 cut(s) 170, 568, 1029, 1083
AlwI GGATC 2 cut(s) 41, 323
AoxI GGCC 2 cut(s) 307, 927
ApeKI GCWGC 5 cut(s) 356, 578, 611, 1111, 1206
ApoI RAATTY 1 cut(s) 301
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 2 cut(s) 61, 670
AsuHPI GGTGA 2 cut(s) 182, 1010
AvaII GGWCC 2 cut(s) 61, 670
BbsI GAAGAC 1 cut(s) 1120
Bbv12I GWGCWC 1 cut(s) 81
BbvCI CCTCAGC 1 cut(s) 234
BbvI GCAGC 5 cut(s) 368, 590, 623, 1098, 1218
BccI CCATC 3 cut(s) 232, 676, 938
BceAI ACGGC 1 cut(s) 267
BclI TGATCA 1 cut(s) 40
BcoDI GTCTC 4 cut(s) 170, 568, 1029, 1083
BfaI CTAG 7 cut(s) 255, 288, 443, 618, 665, 909, 1080
BfmI CTRYAG 2 cut(s) 492, 949
BfoI RGCGCY 1 cut(s) 404
BfrI CTTAAG 1 cut(s) 643
BglII AGATCT 1 cut(s) 125
BisI GCNGC 5 cut(s) 357, 579, 612, 1112, 1207
BlsI GCNGC 5 cut(s) 358, 580, 613, 1113, 1208
Bme18I GGWCC 2 cut(s) 61, 670
BmgT120I GGNCC 2 cut(s) 61, 670
BmiI GGNNCC 1 cut(s) 916
BmsI GCATC 2 cut(s) 1045, 1077
BpiI GAAGAC 1 cut(s) 1120
BplI GAGNNNNNCTC 4 cut(s) 98, 130, 884, 916
Bpu10I CCTNAGC 1 cut(s) 234
BpuEI CTTGAG 3 cut(s) 135, 513, 558
BsaBI GATNNNNATC 2 cut(s) 45, 390
BsaI GGTCTC 1 cut(s) 1029
BsaJI CCNNGG 2 cut(s) 64, 924
Bse1I ACTGG 1 cut(s) 604
Bse3DI GCAATG 3 cut(s) 106, 828, 938
Bse8I GATNNNNATC 2 cut(s) 45, 390
BseDI CCNNGG 2 cut(s) 64, 924
BseGI GGATG 4 cut(s) 10, 220, 391, 1060
BseJI GATNNNNATC 2 cut(s) 45, 390
BseMI GCAATG 3 cut(s) 106, 828, 938
BseMII CTCAG 5 cut(s) 248, 333, 359, 579, 1040
BseNI ACTGG 1 cut(s) 604
BseRI GAGGAG 6 cut(s) 122, 138, 219, 222, 276, 927
BseXI GCAGC 5 cut(s) 368, 590, 623, 1098, 1218
BsgI GTGCAG 1 cut(s) 630
Bsh1236I CGCG 1 cut(s) 75
BshFI GGCC 2 cut(s) 309, 929
BsiHKAI GWGCWC 1 cut(s) 81
BsmAI GTCTC 4 cut(s) 170, 568, 1029, 1083
BsmBI CGTCTC 1 cut(s) 1083
BsnI GGCC 2 cut(s) 309, 929
Bso31I GGTCTC 1 cut(s) 1029
Bsp1286I GDGCHC 1 cut(s) 81
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 4 cut(s) 40, 46, 125, 315
BspACI CCGC 2 cut(s) 73, 704
BspANI GGCC 2 cut(s) 309, 929
BspCNI CTCAG 5 cut(s) 247, 332, 358, 578, 1039
BspFNI CGCG 1 cut(s) 75
BspLI GGNNCC 1 cut(s) 916
BspPI GGATC 2 cut(s) 41, 323
BspTI CTTAAG 1 cut(s) 643
BspTNI GGTCTC 1 cut(s) 1029
BsrDI GCAATG 3 cut(s) 106, 828, 938
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 1 cut(s) 604
BssECI CCNNGG 2 cut(s) 64, 924
BssMI GATC 4 cut(s) 40, 46, 125, 315
Bst4CI ACNGT 5 cut(s) 68, 181, 340, 774, 1142
Bst6I CTCTTC 4 cut(s) 121, 168, 511, 529
BstAFI CTTAAG 1 cut(s) 643
BstAUI TGTACA 1 cut(s) 774
BstC8I GCNNGC 2 cut(s) 77, 1060
BstDEI CTNAG 5 cut(s) 234, 319, 345, 565, 1026
BstDSI CCRYGG 1 cut(s) 64
BstEII GGTNACC 1 cut(s) 68
BstF5I GGATG 4 cut(s) 10, 220, 391, 1060
BstFNI CGCG 1 cut(s) 75
BstH2I RGCGCY 1 cut(s) 404
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 4 cut(s) 43, 49, 128, 318
BstMAI GTCTC 4 cut(s) 170, 568, 1029, 1083
BstMBI GATC 4 cut(s) 40, 46, 125, 315
BstMWI GCNNNNNNNGC 4 cut(s) 578, 661, 935, 1182
BstNSI RCATGY 1 cut(s) 1127
BstPI GGTNACC 1 cut(s) 68
BstSFI CTRYAG 2 cut(s) 492, 949
BstUI CGCG 1 cut(s) 75
BstV1I GCAGC 5 cut(s) 368, 590, 623, 1098, 1218
BstV2I GAAGAC 1 cut(s) 1120
BstX2I RGATCY 3 cut(s) 46, 125, 315
BstYI RGATCY 3 cut(s) 46, 125, 315
BsuRI GGCC 2 cut(s) 309, 929
BtgI CCRYGG 1 cut(s) 64
BtsCI GGATG 4 cut(s) 10, 220, 391, 1060
BtsI GCAGTG 2 cut(s) 918, 1216
BtsIMutI CAGTG 4 cut(s) 336, 597, 918, 1216
Cac8I GCNNGC 2 cut(s) 77, 1060
CfoI GCGC 1 cut(s) 403
Cfr13I GGNCC 2 cut(s) 61, 670
Csp6I GTAC 2 cut(s) 285, 775
CviAII CATG 3 cut(s) 1116, 1124, 1133
CviQI GTAC 2 cut(s) 285, 775
DdeI CTNAG 5 cut(s) 234, 319, 345, 565, 1026
DpnI GATC 4 cut(s) 42, 48, 127, 317
DpnII GATC 4 cut(s) 40, 46, 125, 315
DraIII CACNNNGTG 1 cut(s) 515
Eam1104I CTCTTC 4 cut(s) 121, 168, 511, 529
EarI CTCTTC 4 cut(s) 121, 168, 511, 529
Eco31I GGTCTC 1 cut(s) 1029
Eco47I GGWCC 2 cut(s) 61, 670
Eco57I CTGAAG 1 cut(s) 417
Eco91I GGTNACC 1 cut(s) 68
EcoO65I GGTNACC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 788
Esp3I CGTCTC 1 cut(s) 1083
FaeI CATG 3 cut(s) 1119, 1127, 1136
FalI AAGNNNNNCTT 2 cut(s) 232, 264
FatI CATG 3 cut(s) 1115, 1123, 1132
FauNDI CATATG 1 cut(s) 848
FbaI TGATCA 1 cut(s) 40
FblI GTMKAC 3 cut(s) 948, 1137, 1146
Fnu4HI GCNGC 5 cut(s) 357, 579, 612, 1112, 1207
FokI GGATG 4 cut(s) 17, 207, 378, 1067
Fsp4HI GCNGC 5 cut(s) 357, 579, 612, 1112, 1207
FspBI CTAG 7 cut(s) 255, 288, 443, 618, 665, 909, 1080
GlaI GCGC 1 cut(s) 402
GluI GCNGC 5 cut(s) 357, 579, 612, 1112, 1207
HaeII RGCGCY 1 cut(s) 404
HaeIII GGCC 2 cut(s) 309, 929
HhaI GCGC 1 cut(s) 403
Hin1II CATG 3 cut(s) 1119, 1127, 1136
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HindIII AAGCTT 1 cut(s) 640
HinfI GANTC 5 cut(s) 113, 292, 503, 542, 761
HphI GGTGA 2 cut(s) 182, 1010
Hpy166II GTNNAC 6 cut(s) 88, 546, 670, 949, 1138, 1147
Hpy188I TCNGA 5 cut(s) 348, 760, 884, 991, 1029
Hpy188III TCNNGA 7 cut(s) 44, 123, 134, 313, 395, 679, 890
Hpy8I GTNNAC 6 cut(s) 88, 546, 670, 949, 1138, 1147
Hpy99I CGWCG 1 cut(s) 1097
HpyAV CCTTC 3 cut(s) 765, 989, 995
HpyCH4III ACNGT 5 cut(s) 68, 181, 340, 774, 1142
HpyCH4IV ACGT 2 cut(s) 514, 1036
HpyCH4V TGCA 8 cut(s) 356, 611, 786, 810, 821, 842, 1068, 1185
HpyF10VI GCNNNNNNNGC 4 cut(s) 578, 661, 935, 1182
HpyF3I CTNAG 5 cut(s) 234, 319, 345, 565, 1026
HpySE526I ACGT 2 cut(s) 514, 1036
Hsp92II CATG 3 cut(s) 1119, 1127, 1136
HspAI GCGC 1 cut(s) 401
Ksp22I TGATCA 1 cut(s) 40
Kzo9I GATC 4 cut(s) 40, 46, 125, 315
LmnI GCTCC 1 cut(s) 914
Lsp1109I GCAGC 5 cut(s) 368, 590, 623, 1098, 1218
LweI GCATC 2 cut(s) 1045, 1077
MaeI CTAG 7 cut(s) 255, 288, 443, 618, 665, 909, 1080
MaeII ACGT 2 cut(s) 514, 1036
MaeIII GTNAC 4 cut(s) 68, 155, 334, 743
MalI GATC 4 cut(s) 42, 48, 127, 317
MboI GATC 4 cut(s) 40, 46, 125, 315
MflI RGATCY 3 cut(s) 46, 125, 315
MhlI GDGCHC 1 cut(s) 81
MluCI AATT 1 cut(s) 301
MlyI GAGTC 4 cut(s) 107, 301, 497, 551
MmeI TCCRAC 1 cut(s) 1177
Mph1103I ATGCAT 1 cut(s) 788
MseI TTAA 4 cut(s) 56, 644, 869, 1158
MslI CAYNNNNRTG 2 cut(s) 168, 602
MspA1I CMGCKG 1 cut(s) 1062
MspCI CTTAAG 1 cut(s) 643
MvnI CGCG 1 cut(s) 75
MwoI GCNNNNNNNGC 4 cut(s) 578, 661, 935, 1182
NdeI CATATG 1 cut(s) 848
NdeII GATC 4 cut(s) 40, 46, 125, 315
NlaIII CATG 3 cut(s) 1119, 1127, 1136
NlaIV GGNNCC 1 cut(s) 916
NmeAIII GCCGAG 1 cut(s) 949
NmuCI GTSAC 2 cut(s) 334, 743
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 1 cut(s) 1127
OliI CACNNNNGTG 1 cut(s) 602
PciI ACATGT 1 cut(s) 1123
PcsI WCGNNNNNNNCGW 1 cut(s) 72
PfeI GAWTC 1 cut(s) 761
PkrI GCNGC 5 cut(s) 358, 580, 613, 1113, 1208
PleI GAGTC 4 cut(s) 107, 300, 497, 550
PpsI GAGTC 4 cut(s) 107, 300, 497, 550
PscI ACATGT 1 cut(s) 1123
PsiI TTATAA 1 cut(s) 483
PspEI GGTNACC 1 cut(s) 68
PspN4I GGNNCC 1 cut(s) 916
PspPI GGNCC 2 cut(s) 61, 670
PsrI GAACNNNNNNTAC 2 cut(s) 357, 389
PsuI RGATCY 3 cut(s) 46, 125, 315
PvuII CAGCTG 1 cut(s) 1062
RsaI GTAC 2 cut(s) 286, 776
RsaNI GTAC 2 cut(s) 285, 775
RseI CAYNNNNRTG 2 cut(s) 168, 602
SaqAI TTAA 4 cut(s) 56, 644, 869, 1158
SatI GCNGC 5 cut(s) 357, 579, 612, 1112, 1207
Sau3AI GATC 4 cut(s) 40, 46, 125, 315
Sau96I GGNCC 2 cut(s) 61, 670
SchI GAGTC 4 cut(s) 107, 301, 497, 551
SduI GDGCHC 1 cut(s) 81
SfaNI GCATC 2 cut(s) 1045, 1077
SfcI CTRYAG 2 cut(s) 492, 949
SinI GGWCC 2 cut(s) 61, 670
SmiMI CAYNNNNRTG 2 cut(s) 168, 602
SmlI CTYRAG 4 cut(s) 150, 528, 573, 643
SmoI CTYRAG 4 cut(s) 150, 528, 573, 643
Sse9I AATT 1 cut(s) 301
SsiI CCGC 2 cut(s) 73, 704
SspMI CTAG 7 cut(s) 255, 288, 443, 618, 665, 909, 1080
TaaI ACNGT 5 cut(s) 68, 181, 340, 774, 1142
TaiI ACGT 2 cut(s) 517, 1039
TaqI TCGA 3 cut(s) 135, 487, 889
TasI AATT 1 cut(s) 301
TatI WGTACW 1 cut(s) 774
TfiI GAWTC 1 cut(s) 761
Tru1I TTAA 4 cut(s) 56, 644, 869, 1158
Tru9I TTAA 4 cut(s) 56, 644, 869, 1158
TscAI CASTG 4 cut(s) 343, 604, 925, 1216
TseFI GTSAC 2 cut(s) 334, 743
TseI GCWGC 5 cut(s) 356, 578, 611, 1111, 1206
Tsp45I GTSAC 2 cut(s) 334, 743
TspDTI ATGAA 4 cut(s) 108, 402, 600, 820
TspRI CASTG 4 cut(s) 343, 604, 925, 1216
Vha464I CTTAAG 1 cut(s) 643
VpaK11BI GGWCC 2 cut(s) 61, 670
XapI RAATTY 1 cut(s) 301
XceI RCATGY 1 cut(s) 1127
XcmI CCANNNNNNNNNTGG 2 cut(s) 367, 411
XmiI GTMKAC 3 cut(s) 948, 1137, 1146
XspI CTAG 7 cut(s) 255, 288, 443, 618, 665, 909, 1080
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.